Mercurial > repos > iuc > obi_illumina_pairend
diff illuminapairedend.xml @ 0:7275ce016923 draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/obitools commit e1031e4c94b25d1ed535bf221764ab801b710ab2
author | iuc |
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date | Wed, 12 Apr 2017 17:38:52 -0400 |
parents | |
children | 0c9296704212 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/illuminapairedend.xml Wed Apr 12 17:38:52 2017 -0400 @@ -0,0 +1,70 @@ +<tool id="obi_illumina_pairend" name="Illuminapairedend - Assembling pair-end reads" version="@WRAPPER_VERSION@"> + <description>Construct consensus reads from Illumina pair-end reads</description> + <macros> + <import>macros.xml</import> + </macros> + <expand macro="requirements"/> + <expand macro="stdio"/> + <command> + + <![CDATA[ + illuminapairedend + + --score-min='$score' + -r '$inputfastq3p' + '$inputfastq5p' > '$output' + + ]]> + + </command> + + <inputs> + <param name="inputfastq3p" type="data" format="fastq" label="Read from file" help="file of 3p (1:) Illumina pair-end reads to assemble in sanger fastq nucleic format (standard fastq)" /> + <param name="inputfastq5p" type="data" format="fastq" label="Read from file" help="file of 5p (2:) Illumina pair-end reads to assemble in sanger fastq nucleic format (standard fastq)" /> + <param name="score" type="float" value="40.0" label="minimum score for keeping aligment"/> + </inputs> + <outputs> + <data format="fastq" name="output" label="${tool.name} on ${on_string}: assembly results" /> + </outputs> + + <tests> + <test> + <param name="inputfastq3p" value="wolf_small.F.fastq" /> + <param name="inputfastq5p" value="wolf_small.R.fastq" /> + <param name="score" value="40.0" /> + <output name="output" file="illuminapairedend.output.fastq" ftype="fastq"/> + </test> + </tests> + + <help><![CDATA[ + +.. class:: warning + +**Warning:** +Sequence records corresponding to the same read pair must be in the same order in the two files + +-------- + +.. class:: infomark + +**What it does** + +illuminapairedend aims at aligning the two reads of a pair-end library sequenced using an Illumina platform : + +\* If the two reads overlap, it returns the consensus sequence together with its quality +\* Otherwise, it concatenates sequence merging the forward read and the reversed-complemented reverse read. + +The program uses as input one or two fastq sequences reads files. + +\* If two files are used one of them must be specified using the -r option. Sequence records corresponding to the same read pair must be in the same order in the two files. +\* If just one file is provided, sequence records are supposed to be all of the same length. The first half of th e sequence is used as forward read, the second half is used as the reverse read. + +illuminapairedend align the forward sequence record with the reverse complement of the reverse sequence record. The alignment algorithm takes into account the base qualities. + +@OBITOOLS_LINK@ + +]]> + </help> + <expand macro="citation" /> + +</tool>