annotate test-data/output_obiuniq_family.fasta @ 0:aca6a8c9cf83 draft

planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/obitools commit e1031e4c94b25d1ed535bf221764ab801b710ab2

>HELIUM_000100422_612GNAAXX:7:75:9674:16966#0/2_CONS_SUB_SUB_CMP ali_length=58; sminR=40.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=50; sample=29a_F260619; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; forward_tag=gcctcct; experiment=wolf_diet; seq_a_single=0; merged=['HELIUM_000100422_612GNAAXX:7:75:9674:16966#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:95:11283:2557#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:73:17068:16620#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:96:8737:4973#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:37:12979:6595#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:7:7916:16505#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:13:5742:9859#0/2_CONS_SUB_SUB']; reverse_score=72.0; seq_b_insertion=0; seq_b_deletion=50; seq_a_insertion=0; seq_length_ori=58; reverse_tag=gcctcct; count=7; seq_length=4; status=full; mode=alignment; seq_b_single=0; 
tttt
>HELIUM_000100422_612GNAAXX:7:38:3005:20881#0/2_CONS_SUB_SUB_CMP ali_length=63; seq_ab_match=60; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=26a_F040644; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=227.901935995; seq_a_mismatch=3; forward_tag=gaatatc; seq_b_mismatch=0; experiment=wolf_diet; mid_quality=78.5939849624; avg_quality=77.0784313725; seq_a_single=45; score_norm=3.61749104754; merged=['HELIUM_000100422_612GNAAXX:7:38:3005:20881#0/2_CONS_SUB_SUB_CMP']; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=153; reverse_tag=gaatatc; count=1; seq_length=99; mode=alignment; head_quality=67.0; seq_b_single=45; 
ttagccctaaacatgaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:38:6201:12003#0/2_CONS_SUB_SUB_CMP ali_length=63; seq_ab_match=59; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=26a_F040644; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=219.686655506; seq_a_mismatch=0; forward_tag=gaatatc; seq_b_mismatch=4; experiment=wolf_diet; mid_quality=67.7443609023; avg_quality=67.3986928105; seq_a_single=45; score_norm=3.48708976993; merged=['HELIUM_000100422_612GNAAXX:7:38:6201:12003#0/2_CONS_SUB_SUB_CMP']; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=153; reverse_tag=gaatatc; count=1; seq_length=99; mode=alignment; head_quality=63.2; seq_b_single=45; 
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaata
gcttaaaactcaaagaactgggcggtgctttatatcccg
>HELIUM_000100422_612GNAAXX:7:111:18277:17779#0/2_CONS_SUB_SUB_CMP ali_length=63; seq_ab_match=61; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=26a_F040644; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=235.877819976; seq_a_mismatch=2; forward_tag=gaatatc; seq_b_mismatch=0; experiment=wolf_diet; mid_quality=78.8646616541; avg_quality=77.3137254902; seq_a_single=45; score_norm=3.74409238057; merged=['HELIUM_000100422_612GNAAXX:7:111:18277:17779#0/2_CONS_SUB_SUB_CMP']; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=153; reverse_tag=gaatatc; count=1; seq_length=99; mode=alignment; head_quality=67.0; seq_b_single=45; 
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaata
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:78:7151:19928#0/2_CONS_SUB_SUB ali_length=63; seq_ab_match=56; sminR=40.0; tail_quality=47.6; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=26a_F040644; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=195.576462373; seq_a_mismatch=0; forward_tag=gaatatc; seq_b_mismatch=7; experiment=wolf_diet; mid_quality=58.2105263158; avg_quality=57.1699346405; seq_a_single=45; score_norm=3.10438829164; merged=['HELIUM_000100422_612GNAAXX:7:78:7151:19928#0/2_CONS_SUB_SUB']; reverse_score=72.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=153; reverse_tag=gaatatc; count=1; seq_length=99; status=full; mode=alignment; head_quality=52.9; seq_b_single=45; 
ttagccctaaacataaacattcaataaacgagaatgttcgccagtgtacttctagcaaca
ggctgaaactcaaaggacttggcggtggtttacatccct
>HELIUM_000100422_612GNAAXX:7:76:10822:18722#0/2_CONS_SUB_SUB ali_length=63; seq_ab_match=58; sminR=40.0; tail_quality=66.8; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=26a_F040644; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=211.83390837; seq_a_mismatch=5; forward_tag=gaatatc; seq_b_mismatch=0; experiment=wolf_diet; mid_quality=77.3834586466; avg_quality=75.9803921569; seq_a_single=45; score_norm=3.36244299; merged=['HELIUM_000100422_612GNAAXX:7:76:10822:18722#0/2_CONS_SUB_SUB']; reverse_score=72.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=153; reverse_tag=gaatatc; count=1; seq_length=99; status=full; mode=alignment; head_quality=66.5; seq_b_single=45; 
ttagccctaaacataaacattcaataaacacgaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:53:6550:1163#0/2_CONS_SUB_SUB ali_length=63; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=26a_F040644; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; forward_tag=gaatatc; seq_b_mismatch=0; experiment=wolf_diet; seq_a_single=45; merged=['HELIUM_000100422_612GNAAXX:7:53:6550:1163#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:38:10813:16867#0/2_CONS_SUB_SUB']; status=full; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=153; reverse_tag=gaatatc; count=2; seq_length=99; mode=alignment; head_quality=67.0; seq_b_single=45; 
ttagccctaaacataaacattcaataaacaagaatgtttgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:95:1688:9598#0/2_CONS_SUB_SUB_CMP ali_length=63; seq_ab_match=54; sminR=40.0; tail_quality=66.8; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=26a_F040644; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=179.847313607; seq_a_mismatch=6; forward_tag=gaatatc; seq_b_mismatch=3; experiment=wolf_diet; mid_quality=74.2932330827; avg_quality=73.2418300654; seq_a_single=45; score_norm=2.8547192636; merged=['HELIUM_000100422_612GNAAXX:7:95:1688:9598#0/2_CONS_SUB_SUB_CMP']; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=153; reverse_tag=gaatatc; count=1; seq_length=99; mode=alignment; head_quality=65.7; seq_b_single=45; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtgatactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:7:11998:4462#0/2_CONS_SUB_SUB ali_length=63; seq_ab_match=59; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=26a_F040644; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=219.864332815; seq_a_mismatch=4; forward_tag=gaatatc; seq_b_mismatch=0; experiment=wolf_diet; mid_quality=78.2105263158; avg_quality=76.7450980392; seq_a_single=45; score_norm=3.48991004469; merged=['HELIUM_000100422_612GNAAXX:7:7:11998:4462#0/2_CONS_SUB_SUB']; reverse_score=72.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=153; reverse_tag=gaatatc; count=1; seq_length=99; status=full; mode=alignment; head_quality=67.0; seq_b_single=45; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtagtactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:115:14354:10118#0/2_CONS_SUB_SUB_CMP ali_length=63; seq_ab_match=59; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=26a_F040644; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=219.850378027; seq_a_mismatch=2; forward_tag=gaatatc; seq_b_mismatch=2; experiment=wolf_diet; mid_quality=78.045112782; avg_quality=76.6013071895; seq_a_single=45; score_norm=3.4896885401; merged=['HELIUM_000100422_612GNAAXX:7:115:14354:10118#0/2_CONS_SUB_SUB_CMP']; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=153; reverse_tag=gaatatc; count=1; seq_length=99; mode=alignment; head_quality=67.0; seq_b_single=45; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactcgcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:111:8478:1127#0/2_CONS_SUB_SUB_CMP ali_length=63; seq_ab_match=57; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=26a_F040644; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=203.794981474; seq_a_mismatch=5; forward_tag=gaatatc; seq_b_mismatch=1; experiment=wolf_diet; mid_quality=76.3909774436; avg_quality=75.1241830065; seq_a_single=45; score_norm=3.23484097578; merged=['HELIUM_000100422_612GNAAXX:7:111:8478:1127#0/2_CONS_SUB_SUB_CMP']; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=153; reverse_tag=gaatatc; count=1; seq_length=99; mode=alignment; head_quality=66.4; seq_b_single=45; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctggaacgcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:16:16357:19948#0/2_CONS_SUB_SUB ali_length=63; seq_ab_match=57; sminR=40.0; tail_quality=66.7; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=26a_F040644; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=203.860719115; seq_a_mismatch=5; forward_tag=gaatatc; seq_b_mismatch=1; experiment=wolf_diet; mid_quality=75.6541353383; avg_quality=74.1764705882; seq_a_single=45; score_norm=3.23588443039; merged=['HELIUM_000100422_612GNAAXX:7:16:16357:19948#0/2_CONS_SUB_SUB']; reverse_score=72.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=153; reverse_tag=gaatatc; count=1; seq_length=99; status=full; mode=alignment; head_quality=62.0; seq_b_single=45; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcacaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:19:6313:16567#0/2_CONS_SUB_SUB_CMP ali_length=63; seq_ab_match=60; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=26a_F040644; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=227.826165596; seq_a_mismatch=3; forward_tag=gaatatc; seq_b_mismatch=0; experiment=wolf_diet; mid_quality=77.4135338346; avg_quality=76.0261437908; seq_a_single=45; score_norm=3.61628834279; merged=['HELIUM_000100422_612GNAAXX:7:19:6313:16567#0/2_CONS_SUB_SUB_CMP']; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=153; reverse_tag=gaatatc; count=1; seq_length=99; mode=alignment; head_quality=66.6; seq_b_single=45; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaagggcttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:89:9710:20652#0/2_CONS_SUB_SUB ali_length=63; seq_ab_match=62; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=26a_F040644; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=243.910293117; seq_a_mismatch=0; forward_tag=gaatatc; seq_b_mismatch=1; experiment=wolf_diet; mid_quality=78.8421052632; avg_quality=77.2941176471; seq_a_single=45; score_norm=3.87159195424; merged=['HELIUM_000100422_612GNAAXX:7:89:9710:20652#0/2_CONS_SUB_SUB']; reverse_score=72.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=153; reverse_tag=gaatatc; count=1; seq_length=99; status=full; mode=alignment; head_quality=67.0; seq_b_single=45; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:104:10274:7999#0/2_CONS_SUB_SUB ali_length=63; sminR=40.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=26a_F040644; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; forward_tag=gaatatc; experiment=wolf_diet; seq_a_single=45; merged=['HELIUM_000100422_612GNAAXX:7:104:10274:7999#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:30:16081:4486#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:29:1328:13185#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:4:10139:2389#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:18:11865:6418#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:42:12961:8650#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:38:9654:18700#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:17:11935:17343#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:47:11864:1343#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:21:2093:4412#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:6:6617:2744#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:47:8320:8571#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:115:3396:14235#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:115:6263:17700#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:13:3469:18630#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:18:2721:16829#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:91:10071:8960#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:81:17261:2872#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:86:15907:21191#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:111:15484:19248#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:29:12308:10119#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:99:14794:3606#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:109:17917:15159#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:35:12723:18557#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:10:4085:7492#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:119:2660:19129#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:2:6320:8068#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:102:6364:4627#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:83:18882:9967#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:85:2105:21152#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:32:18486:7018#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:46:13269:4134#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:48:7139:3789#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:42:8435:4155#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:67:7582:3642#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:74:4888:6953#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:95:17941:6113#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:74:8396:18135#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:95:3274:5435#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:111:7660:7465#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:48:5296:16375#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:72:8905:10715#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:4:18261:9148#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:103:8847:6326#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:29:3531:8314#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:49:11740:5547#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:30:5374:18213#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:41:2354:1449#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:111:7441:14688#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:34:18733:3827#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:58:15837:8531#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:117:2416:10342#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:11:8547:18238#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:117:4291:18111#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:69:7696:12881#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:118:13803:15594#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:16:5047:7843#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:117:12470:18434#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:84:11257:18886#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:115:3921:10649#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:109:17335:11429#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:3:14624:12375#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:74:8683:20738#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:57:18459:16145#0/2_CONS_SUB_SUB_CMP']; reverse_score=72.0; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=153; reverse_tag=gaatatc; count=64; seq_length=99; status=full; mode=alignment; seq_b_single=45; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:72:12959:5736#0/2_CONS_SUB_SUB_CMP ali_length=63; seq_ab_match=56; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=26a_F040644; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=195.664863172; seq_a_mismatch=0; forward_tag=gaatatc; seq_b_mismatch=7; experiment=wolf_diet; mid_quality=63.5263157895; avg_quality=63.5098039216; seq_a_single=45; score_norm=3.10579147892; merged=['HELIUM_000100422_612GNAAXX:7:72:12959:5736#0/2_CONS_SUB_SUB_CMP']; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=153; reverse_tag=gaatatc; count=1; seq_length=99; mode=alignment; head_quality=59.8; seq_b_single=45; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacacccct
>HELIUM_000100422_612GNAAXX:7:81:15726:2345#0/2_CONS_SUB_SUB_CMP ali_length=63; seq_ab_match=56; sminR=40.0; tail_quality=62.3; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=26a_F040644; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=195.641193686; seq_a_mismatch=0; forward_tag=gaatatc; seq_b_mismatch=7; experiment=wolf_diet; mid_quality=63.1954887218; avg_quality=63.091503268; seq_a_single=45; score_norm=3.10541577279; merged=['HELIUM_000100422_612GNAAXX:7:81:15726:2345#0/2_CONS_SUB_SUB_CMP']; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=153; reverse_tag=gaatatc; count=1; seq_length=99; mode=alignment; head_quality=62.5; seq_b_single=45; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaagcactcggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:120:16005:7955#0/2_CONS_SUB_SUB_CMP ali_length=64; seq_ab_match=61; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=26a_F040644; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=231.880428082; seq_a_mismatch=1; forward_tag=gaatatc; seq_b_mismatch=2; experiment=wolf_diet; mid_quality=78.8939393939; avg_quality=77.3289473684; seq_a_single=44; score_norm=3.62313168878; merged=['HELIUM_000100422_612GNAAXX:7:120:16005:7955#0/2_CONS_SUB_SUB_CMP']; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=152; reverse_tag=gaatatc; count=1; seq_length=98; mode=alignment; head_quality=67.0; seq_b_single=44; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gccgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:19:4311:13343#0/2_CONS_SUB_SUB_CMP ali_length=63; seq_ab_match=58; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=26a_F040644; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=211.837822462; seq_a_mismatch=4; forward_tag=gaatatc; seq_b_mismatch=1; experiment=wolf_diet; mid_quality=77.7969924812; avg_quality=76.385620915; seq_a_single=45; score_norm=3.36250511845; merged=['HELIUM_000100422_612GNAAXX:7:19:4311:13343#0/2_CONS_SUB_SUB_CMP']; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=153; reverse_tag=gaatatc; count=1; seq_length=99; mode=alignment; head_quality=67.0; seq_b_single=45; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactacgagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:13:6954:13039#0/2_CONS_SUB_SUB_CMP ali_length=63; seq_ab_match=62; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=26a_F040644; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=243.872474161; seq_a_mismatch=1; forward_tag=gaatatc; seq_b_mismatch=0; experiment=wolf_diet; mid_quality=79.0; avg_quality=77.431372549; seq_a_single=45; score_norm=3.87099165336; merged=['HELIUM_000100422_612GNAAXX:7:13:6954:13039#0/2_CONS_SUB_SUB_CMP']; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=153; reverse_tag=gaatatc; count=1; seq_length=99; mode=alignment; head_quality=67.0; seq_b_single=45; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagaggactactagcaata
gcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:116:14244:17926#0/2_CONS_SUB_SUB ali_length=63; seq_ab_match=55; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=26a_F040644; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=187.882758912; seq_a_mismatch=5; forward_tag=gaatatc; seq_b_mismatch=3; experiment=wolf_diet; mid_quality=76.5338345865; avg_quality=75.2875816993; seq_a_single=45; score_norm=2.98226601448; merged=['HELIUM_000100422_612GNAAXX:7:116:14244:17926#0/2_CONS_SUB_SUB']; reverse_score=72.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=153; reverse_tag=gaatatc; count=1; seq_length=99; status=full; mode=alignment; head_quality=67.0; seq_b_single=45; 
ttagccctaaacataaacattcaataaacaagaatgttcgcaagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:7:15538:8325#0/2_CONS_SUB_SUB_CMP ali_length=63; seq_ab_match=52; sminR=40.0; tail_quality=63.4; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=26a_F040644; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=151.662770749; seq_a_mismatch=0; forward_tag=gaatatc; seq_b_mismatch=10; experiment=wolf_diet; mid_quality=60.1879699248; avg_quality=60.3333333333; seq_a_single=45; score_norm=2.40734556744; merged=['HELIUM_000100422_612GNAAXX:7:7:15538:8325#0/2_CONS_SUB_SUB_CMP']; status=full; direction=reverse; seq_b_insertion=1; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=153; reverse_tag=gaatatc; count=1; seq_length=99; mode=alignment; head_quality=59.2; seq_b_single=45; 
ttagccctaaacataaacattcaataaacaagaatgtcggccagagtactactagcaaca
gcatgaaactcaaagaactgggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:29:18416:16620#0/2_CONS_SUB_SUB_CMP ali_length=62; seq_ab_match=60; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=29a_F260619; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=231.873033258; seq_a_mismatch=2; forward_tag=gcctcct; seq_b_mismatch=0; experiment=wolf_diet; mid_quality=77.4179104478; avg_quality=76.0649350649; seq_a_single=46; score_norm=3.7398876332; merged=['HELIUM_000100422_612GNAAXX:7:29:18416:16620#0/2_CONS_SUB_SUB_CMP']; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=gcctcct; count=1; seq_length=100; mode=alignment; head_quality=67.0; seq_b_single=46; 
ttagccctaaacacaagtaattattataacaaaattattcgccagagttctaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctg
>HELIUM_000100422_612GNAAXX:7:46:9246:19171#0/2_CONS_SUB_SUB ali_length=62; sminR=40.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=29a_F260619; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; forward_tag=gcctcct; experiment=wolf_diet; seq_a_single=46; merged=['HELIUM_000100422_612GNAAXX:7:46:9246:19171#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:29:15520:18035#0/2_CONS_SUB_SUB']; status=full; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=gcctcct; count=2; seq_length=100; mode=alignment; seq_b_single=46; 
ttagccctaaacacaagtaattattataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:119:14871:19157#0/2_CONS_SUB_SUB_CMP ali_length=62; seq_ab_match=47; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=29a_F260619; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=115.761290673; seq_a_mismatch=1; forward_tag=gcctcct; seq_b_mismatch=13; experiment=wolf_diet; mid_quality=69.1492537313; avg_quality=68.8701298701; seq_a_single=46; score_norm=1.86711759151; merged=['HELIUM_000100422_612GNAAXX:7:119:14871:19157#0/2_CONS_SUB_SUB_CMP']; status=full; direction=reverse; seq_b_insertion=1; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=154; reverse_tag=gcctcct; count=1; seq_length=100; mode=alignment; head_quality=67.0; seq_b_single=46; 
ttagccctaaacacaagtaattattataacaaaatcattcgccagagtactaccggcaat
agctcaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:42:19757:3800#0/2_CONS_SUB_SUB_CMP ali_length=62; seq_ab_match=57; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=1; sample=15a_F730814; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=187.78301321; seq_a_mismatch=1; forward_tag=gaagtag; seq_b_mismatch=3; experiment=wolf_diet; mid_quality=77.7669172932; avg_quality=76.3660130719; seq_a_single=45; score_norm=3.02875827758; merged=['HELIUM_000100422_612GNAAXX:7:42:19757:3800#0/2_CONS_SUB_SUB_CMP']; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=1; seq_a_insertion=1; seq_length_ori=153; reverse_tag=gaagtag; count=1; seq_length=99; mode=alignment; head_quality=67.1; seq_b_single=46; 
ttagccctaaacacaagtaattaatatacaaaattattcgccagagtactaccggcaata
gcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:108:5640:3823#0/2_CONS_SUB_SUB_CMP ali_length=62; seq_ab_match=60; sminR=40.0; tail_quality=66.9; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=29a_F260619; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=231.881364714; seq_a_mismatch=0; forward_tag=gcctcct; seq_b_mismatch=2; experiment=wolf_diet; mid_quality=78.447761194; avg_quality=76.9545454545; seq_a_single=46; score_norm=3.74002201152; merged=['HELIUM_000100422_612GNAAXX:7:108:5640:3823#0/2_CONS_SUB_SUB_CMP']; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=gcctcct; count=1; seq_length=100; mode=alignment; head_quality=67.0; seq_b_single=46; 
ttagccctaaacacaagtaattaatataacaaaattgttcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:59:11971:12063#0/2_CONS_SUB_SUB ali_length=62; seq_ab_match=62; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=29a_F260619; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=247.995051728; seq_a_mismatch=0; forward_tag=gcctcct; seq_b_mismatch=0; experiment=wolf_diet; mid_quality=78.9850746269; avg_quality=77.4285714286; seq_a_single=46; score_norm=3.99992018915; merged=['HELIUM_000100422_612GNAAXX:7:59:11971:12063#0/2_CONS_SUB_SUB']; reverse_score=72.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=gcctcct; count=1; seq_length=100; status=full; mode=alignment; head_quality=67.0; seq_b_single=46; 
ttagccctaaacacaagtaattaatataacaaaattattcgtcagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:76:8491:8900#0/2_CONS_SUB_SUB_CMP ali_length=62; seq_ab_match=57; sminR=40.0; tail_quality=65.3; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=15a_F730814; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=207.828655885; seq_a_mismatch=4; forward_tag=gaagtag; seq_b_mismatch=1; experiment=wolf_diet; mid_quality=76.552238806; avg_quality=75.1948051948; seq_a_single=46; score_norm=3.35207509492; merged=['HELIUM_000100422_612GNAAXX:7:76:8491:8900#0/2_CONS_SUB_SUB_CMP']; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=gaagtag; count=1; seq_length=100; mode=alignment; head_quality=66.9; seq_b_single=46; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtgctacctgcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:114:4511:17596#0/2_CONS_SUB_SUB_CMP ali_length=62; seq_ab_match=57; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=29a_F260619; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=207.883730153; seq_a_mismatch=4; forward_tag=gcctcct; seq_b_mismatch=1; experiment=wolf_diet; mid_quality=75.3208955224; avg_quality=74.2532467532; seq_a_single=46; score_norm=3.35296338956; merged=['HELIUM_000100422_612GNAAXX:7:114:4511:17596#0/2_CONS_SUB_SUB_CMP']; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=gcctcct; count=1; seq_length=100; mode=alignment; head_quality=67.2; seq_b_single=46; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtgctaccggcaat
agcttaaaactcaaaggacttggcggtgctttatacgctt
>HELIUM_000100422_612GNAAXX:7:7:11110:17924#0/2_CONS_SUB_SUB ali_length=62; seq_ab_match=58; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=15a_F730814; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=215.812113201; seq_a_mismatch=3; forward_tag=gaagtag; seq_b_mismatch=1; experiment=wolf_diet; mid_quality=75.723880597; avg_quality=74.5519480519; seq_a_single=46; score_norm=3.4808405355; merged=['HELIUM_000100422_612GNAAXX:7:7:11110:17924#0/2_CONS_SUB_SUB']; reverse_score=72.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=gaagtag; count=1; seq_length=100; status=full; mode=alignment; head_quality=66.4; seq_b_single=46; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtcataccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:3:17077:6562#0/2_CONS_SUB_SUB_CMP ali_length=62; seq_ab_match=59; sminR=40.0; tail_quality=66.6; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=15a_F730814; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=223.876050008; seq_a_mismatch=3; forward_tag=gaagtag; seq_b_mismatch=0; experiment=wolf_diet; mid_quality=77.6567164179; avg_quality=76.1818181818; seq_a_single=46; score_norm=3.61090403239; merged=['HELIUM_000100422_612GNAAXX:7:3:17077:6562#0/2_CONS_SUB_SUB_CMP']; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=gaagtag; count=1; seq_length=100; mode=alignment; head_quality=66.0; seq_b_single=46; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactgccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:120:1900:13295#0/2_CONS_SUB_SUB_CMP ali_length=62; seq_ab_match=60; sminR=40.0; tail_quality=67.1; reverse_match=tagaacaggctcctctag; seq_a_deletion=1; sample=15a_F730814; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=219.874676069; seq_a_mismatch=1; forward_tag=gaagtag; seq_b_mismatch=0; experiment=wolf_diet; mid_quality=77.5149253731; avg_quality=76.1558441558; seq_a_single=46; score_norm=3.54636574304; merged=['HELIUM_000100422_612GNAAXX:7:120:1900:13295#0/2_CONS_SUB_SUB_CMP']; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=1; seq_length_ori=154; reverse_tag=gaagtag; count=1; seq_length=100; mode=alignment; head_quality=67.0; seq_b_single=46; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactacgcgcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:14:3978:11028#0/2_CONS_SUB_SUB_CMP ali_length=62; sminR=40.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; seq_b_mismatch=0; experiment=wolf_diet; seq_a_single=46; merged=['HELIUM_000100422_612GNAAXX:7:14:3978:11028#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:115:17402:4174#0/2_CONS_SUB_SUB_CMP']; reverse_score=72.0; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; count=2; seq_length=100; status=full; mode=alignment; seq_b_single=46; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactacctgcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:71:17473:7401#0/2_CONS_SUB_SUB_CMP ali_length=62; seq_ab_match=60; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=15a_F730814; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=231.883938289; seq_a_mismatch=1; forward_tag=gaagtag; seq_b_mismatch=1; experiment=wolf_diet; mid_quality=77.2089552239; avg_quality=75.7857142857; seq_a_single=46; score_norm=3.74006352079; merged=['HELIUM_000100422_612GNAAXX:7:71:17473:7401#0/2_CONS_SUB_SUB_CMP']; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=gaagtag; count=1; seq_length=100; mode=alignment; head_quality=65.5; seq_b_single=46; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggtaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:24:16230:12703#0/2_CONS_SUB_SUB_CMP ali_length=62; seq_ab_match=59; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=15a_F730814; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=223.86908915; seq_a_mismatch=2; forward_tag=gaagtag; seq_b_mismatch=1; experiment=wolf_diet; mid_quality=77.4552238806; avg_quality=76.0519480519; seq_a_single=46; score_norm=3.61079176049; merged=['HELIUM_000100422_612GNAAXX:7:24:16230:12703#0/2_CONS_SUB_SUB_CMP']; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=gaagtag; count=1; seq_length=100; mode=alignment; head_quality=66.3; seq_b_single=46; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttatcccctt
>HELIUM_000100422_612GNAAXX:7:13:17353:13234#0/2_CONS_SUB_SUB_CMP ali_length=62; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=15a_F730814; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; seq_a_mismatch=1; forward_tag=gaagtag; experiment=wolf_diet; seq_a_single=46; merged=['HELIUM_000100422_612GNAAXX:7:13:17353:13234#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:70:11798:2668#0/2_CONS_SUB_SUB']; status=full; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=gaagtag; count=2; seq_length=100; mode=alignment; seq_b_single=46; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccttt
>HELIUM_000100422_612GNAAXX:7:14:7706:12338#0/2_CONS_SUB_SUB_CMP sminR=40.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; experiment=wolf_diet; merged=['HELIUM_000100422_612GNAAXX:7:14:7706:12338#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:59:15367:8376#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:74:4783:20479#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:16:19299:8684#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:66:11860:20419#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:36:10388:10324#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:101:11720:19906#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:67:5288:13403#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:17:16877:9760#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:53:2128:13141#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:7:17828:13825#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:58:8591:17028#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:2:7296:17275#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:61:17484:5085#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:19:11039:3957#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:16:15509:18069#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:99:13467:17714#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:1:14576:14181#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:25:11691:21134#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:74:8297:20108#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:54:2542:19180#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:82:6036:6921#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:46:11654:6037#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:15:16118:15111#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:28:9406:18226#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:63:4725:12502#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:113:2918:18736#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:33:9296:13526#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:53:10153:11845#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:73:16525:10764#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:83:6795:14061#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:41:16707:19819#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:38:13721:2004#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:99:14742:5591#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:41:19763:3556#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:40:3610:8896#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:44:19605:15509#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:93:18556:10479#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:96:19317:7551#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:110:17877:11976#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:108:2729:8600#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:114:10615:11217#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:94:12367:17729#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:85:18986:17662#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:86:10092:15563#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:65:6787:8456#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:68:19115:18847#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:71:14800:3222#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:52:10700:16508#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:53:14379:5922#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:78:18994:16521#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:11:8428:13716#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:35:3798:1896#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:72:13060:5051#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:109:12848:8882#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:120:14963:1929#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:108:17701:18887#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:50:2791:11138#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:89:10621:20317#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:111:2893:6772#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:22:8540:14708#0/2_CONS_SUB_SUB']; status=full; seq_length_ori=154; count=61; seq_length=100; mode=alignment; seq_b_single=46; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:107:3570:18147#0/2_CONS_SUB_SUB_CMP ali_length=62; seq_ab_match=60; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=29a_F260619; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=231.924768153; seq_a_mismatch=2; forward_tag=gcctcct; seq_b_mismatch=0; experiment=wolf_diet; mid_quality=78.0820895522; avg_quality=76.5714285714; seq_a_single=46; score_norm=3.74072206698; merged=['HELIUM_000100422_612GNAAXX:7:107:3570:18147#0/2_CONS_SUB_SUB_CMP']; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=gcctcct; count=1; seq_length=100; mode=alignment; head_quality=65.9; seq_b_single=46; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcataaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:97:14311:19299#0/2_CONS_SUB_SUB_CMP ali_length=62; seq_ab_match=52; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=29a_F260619; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=167.91734192; seq_a_mismatch=5; forward_tag=gcctcct; seq_b_mismatch=5; experiment=wolf_diet; mid_quality=75.0820895522; avg_quality=74.0064935065; seq_a_single=46; score_norm=2.70834422451; merged=['HELIUM_000100422_612GNAAXX:7:97:14311:19299#0/2_CONS_SUB_SUB_CMP']; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=gcctcct; count=1; seq_length=100; mode=alignment; head_quality=66.6; seq_b_single=46; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaag
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:25:7789:3090#0/2_CONS_SUB_SUB_CMP ali_length=62; seq_ab_match=59; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=29a_F260619; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=223.705772044; seq_a_mismatch=0; forward_tag=gcctcct; seq_b_mismatch=3; experiment=wolf_diet; mid_quality=72.4179104478; avg_quality=71.6818181818; seq_a_single=46; score_norm=3.60815761361; merged=['HELIUM_000100422_612GNAAXX:7:25:7789:3090#0/2_CONS_SUB_SUB_CMP']; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=gcctcct; count=1; seq_length=100; mode=alignment; head_quality=66.5; seq_b_single=46; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactacaggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:105:14135:6905#0/2_CONS_SUB_SUB ali_length=62; seq_ab_match=61; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=29a_F260619; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=239.985379811; seq_a_mismatch=1; forward_tag=gcctcct; seq_b_mismatch=0; experiment=wolf_diet; mid_quality=78.8134328358; avg_quality=77.2792207792; seq_a_single=46; score_norm=3.87073193244; merged=['HELIUM_000100422_612GNAAXX:7:105:14135:6905#0/2_CONS_SUB_SUB']; reverse_score=72.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=gcctcct; count=1; seq_length=100; status=full; mode=alignment; head_quality=67.0; seq_b_single=46; 
ttagccctaaacacaagtaattaatataacaaaattattcaccagagtactagcggcaac
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:111:19168:18517#0/2_CONS_SUB_SUB ali_length=63; seq_ab_match=60; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=15a_F730814; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=223.825469474; seq_a_mismatch=1; forward_tag=gaagtag; seq_b_mismatch=1; experiment=wolf_diet; mid_quality=77.8955223881; avg_quality=76.4805194805; seq_a_single=45; score_norm=3.55278522975; merged=['HELIUM_000100422_612GNAAXX:7:111:19168:18517#0/2_CONS_SUB_SUB']; reverse_score=72.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=1; seq_length_ori=154; reverse_tag=gaagtag; count=1; seq_length=100; status=full; mode=alignment; head_quality=67.0; seq_b_single=46; 
ttagccctaaacacaagtaattaatataacaaaattatccgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:10:9237:10532#0/2_CONS_SUB_SUB_CMP ali_length=62; seq_ab_match=57; sminR=40.0; tail_quality=66.9; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=29a_F260619; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=207.90944986; seq_a_mismatch=2; forward_tag=gcctcct; seq_b_mismatch=3; experiment=wolf_diet; mid_quality=75.9402985075; avg_quality=74.7532467532; seq_a_single=46; score_norm=3.35337822355; merged=['HELIUM_000100422_612GNAAXX:7:10:9237:10532#0/2_CONS_SUB_SUB_CMP']; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=gcctcct; count=1; seq_length=100; mode=alignment; head_quality=66.7; seq_b_single=46; 
ttagccctaaacacaagtaattaatataacaaaataattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:30:1800:7833#0/2_CONS_SUB_SUB ali_length=62; seq_ab_match=52; sminR=40.0; tail_quality=65.6; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=15a_F730814; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=167.712615339; seq_a_mismatch=0; forward_tag=gaagtag; seq_b_mismatch=10; experiment=wolf_diet; mid_quality=59.9104477612; avg_quality=58.6623376623; seq_a_single=46; score_norm=2.70504218289; merged=['HELIUM_000100422_612GNAAXX:7:30:1800:7833#0/2_CONS_SUB_SUB']; reverse_score=72.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=gaagtag; count=1; seq_length=100; status=full; mode=alignment; head_quality=35.0; seq_b_single=46; 
ttagccctaaacacaagacattaatataacgagattaatcgacagagtactaccggctat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:41:9316:2256#0/2_CONS_SUB_SUB ali_length=62; seq_ab_match=61; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=29a_F260619; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=239.993848494; seq_a_mismatch=1; forward_tag=gcctcct; seq_b_mismatch=0; experiment=wolf_diet; mid_quality=78.6343283582; avg_quality=77.1233766234; seq_a_single=46; score_norm=3.87086852409; merged=['HELIUM_000100422_612GNAAXX:7:41:9316:2256#0/2_CONS_SUB_SUB']; reverse_score=72.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=gcctcct; count=1; seq_length=100; status=full; mode=alignment; head_quality=67.0; seq_b_single=46; 
ttagccctaaacacaagaaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:85:13257:14594#0/2_CONS_SUB_SUB_CMP ali_length=62; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=29a_F260619; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; seq_a_mismatch=2; forward_tag=gcctcct; experiment=wolf_diet; seq_a_single=46; merged=['HELIUM_000100422_612GNAAXX:7:85:13257:14594#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:14:15397:17819#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:89:10281:5930#0/2_CONS_SUB_SUB_CMP']; reverse_score=72.0; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=gcctcct; count=3; seq_length=100; status=full; mode=alignment; seq_b_single=46; 
ttagccctaaacacaaataattacacaaacaaaattgttcaccagagtactagcggcaac
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:76:18268:14980#0/2_CONS_SUB_SUB_CMP ali_length=62; seq_ab_match=61; sminR=40.0; tail_quality=66.9; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=29a_F260619; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=239.985254914; seq_a_mismatch=1; forward_tag=gcctcct; seq_b_mismatch=0; experiment=wolf_diet; mid_quality=78.3208955224; avg_quality=76.8441558442; seq_a_single=46; score_norm=3.87072991797; merged=['HELIUM_000100422_612GNAAXX:7:76:18268:14980#0/2_CONS_SUB_SUB_CMP']; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=gcctcct; count=1; seq_length=100; mode=alignment; head_quality=67.0; seq_b_single=46; 
ttacccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:108:9222:18258#0/2_CONS_SUB_SUB ali_length=62; seq_ab_match=60; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=13a_F730603; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=231.857703869; seq_a_mismatch=0; forward_tag=aattaac; seq_b_mismatch=2; experiment=wolf_diet; mid_quality=78.5; avg_quality=77.0194805195; seq_a_single=46; score_norm=3.73964038498; merged=['HELIUM_000100422_612GNAAXX:7:108:9222:18258#0/2_CONS_SUB_SUB']; reverse_score=72.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=aattaac; count=1; seq_length=100; status=full; mode=alignment; head_quality=67.2; seq_b_single=46; 
ctagccttaaacacaaatagttatgcagacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:103:3594:15996#0/2_CONS_SUB_SUB ali_length=62; seq_ab_match=60; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=13a_F730603; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=231.92194325; seq_a_mismatch=2; forward_tag=aattaac; seq_b_mismatch=0; experiment=wolf_diet; mid_quality=78.3805970149; avg_quality=76.9090909091; seq_a_single=46; score_norm=3.74067650404; merged=['HELIUM_000100422_612GNAAXX:7:103:3594:15996#0/2_CONS_SUB_SUB']; reverse_score=72.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=aattaac; count=1; seq_length=100; status=full; mode=alignment; head_quality=67.1; seq_b_single=46; 
ctagccttaaacacaaatagttatgcaaacacaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:22:1798:3790#0/2_CONS_SUB_SUB_CMP ali_length=62; seq_ab_match=59; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=13a_F730603; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=223.82253574; seq_a_mismatch=3; forward_tag=aattaac; seq_b_mismatch=0; experiment=wolf_diet; mid_quality=78.3208955224; avg_quality=76.8506493506; seq_a_single=46; score_norm=3.61004089903; merged=['HELIUM_000100422_612GNAAXX:7:22:1798:3790#0/2_CONS_SUB_SUB_CMP']; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=aattaac; count=1; seq_length=100; mode=alignment; head_quality=67.0; seq_b_single=46; 
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtgctaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:55:16528:11418#0/2_CONS_SUB_SUB ali_length=62; seq_ab_match=58; sminR=40.0; tail_quality=57.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=13a_F730603; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=215.988419267; seq_a_mismatch=4; forward_tag=aattaac; seq_b_mismatch=0; experiment=wolf_diet; mid_quality=76.0746268657; avg_quality=74.2142857143; seq_a_single=46; score_norm=3.48368418172; merged=['HELIUM_000100422_612GNAAXX:7:55:16528:11418#0/2_CONS_SUB_SUB']; reverse_score=72.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=aattaac; count=1; seq_length=100; status=full; mode=alignment; head_quality=66.5; seq_b_single=46; 
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcact
agcttacaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:54:14618:14353#0/2_CONS_SUB_SUB_CMP sminR=40.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=13a_F730603; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; forward_tag=aattaac; experiment=wolf_diet; merged=['HELIUM_000100422_612GNAAXX:7:54:14618:14353#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:116:12598:9964#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:43:8022:8427#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:88:15797:19191#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:18:5240:1587#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:12:9609:14740#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:16:16055:12835#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:72:7057:20448#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:32:15197:9940#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:53:18419:8404#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:89:16663:15081#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:43:7980:7908#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:63:18851:7688#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:77:18518:10758#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:86:11013:15234#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:59:18371:8149#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:23:13304:5167#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:97:10495:17046#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:120:4726:20808#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:100:17253:5513#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:46:1592:11866#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:58:18618:20959#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:13:14386:20412#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:114:8865:19510#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:89:16162:8889#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:21:5487:19582#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:47:3878:6141#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:78:10338:12302#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:47:7619:9866#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:97:9416:9155#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:32:18262:6966#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:82:8836:11354#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:16:4418:5128#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:71:18821:20471#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:45:6831:6228#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:32:4360:3451#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:33:16506:1490#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:21:4713:10229#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:44:12484:5320#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:19:4865:20715#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:7:10646:8487#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:18:5317:11571#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:12:17693:3451#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:97:9091:15775#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:97:10414:9929#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:6:9274:14951#0/2_CONS_SUB_SUB']; reverse_score=72.0; seq_b_deletion=0; seq_a_insertion=0; reverse_tag=aattaac; count=46; seq_length=100; status=full; mode=alignment; 
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:99:8183:13912#0/2_CONS_SUB_SUB ali_length=62; seq_ab_match=60; sminR=40.0; tail_quality=67.1; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=13a_F730603; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=231.903850642; seq_a_mismatch=1; forward_tag=aattaac; seq_b_mismatch=1; experiment=wolf_diet; mid_quality=78.3358208955; avg_quality=76.8701298701; seq_a_single=46; score_norm=3.74038468777; merged=['HELIUM_000100422_612GNAAXX:7:99:8183:13912#0/2_CONS_SUB_SUB']; reverse_score=72.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=aattaac; count=1; seq_length=100; status=full; mode=alignment; head_quality=67.0; seq_b_single=46; 
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctc
>HELIUM_000100422_612GNAAXX:7:4:4214:9434#0/2_CONS_SUB_SUB ali_length=62; seq_ab_match=58; sminR=40.0; tail_quality=63.3; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=13a_F730603; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=215.812703412; seq_a_mismatch=3; forward_tag=aattaac; seq_b_mismatch=1; experiment=wolf_diet; mid_quality=76.2388059701; avg_quality=74.6883116883; seq_a_single=46; score_norm=3.48085005502; merged=['HELIUM_000100422_612GNAAXX:7:4:4214:9434#0/2_CONS_SUB_SUB']; reverse_score=72.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=aattaac; count=1; seq_length=100; status=full; mode=alignment; head_quality=65.3; seq_b_single=46; 
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactacaggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:9:10358:4089#0/2_CONS_SUB_SUB_CMP ali_length=61; seq_ab_match=54; sminR=40.0; tail_quality=66.9; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=13a_F730603; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=181.620394786; seq_a_mismatch=1; forward_tag=aattaac; seq_b_mismatch=6; experiment=wolf_diet; mid_quality=66.3880597015; avg_quality=66.4545454545; seq_a_single=46; score_norm=2.97738352108; merged=['HELIUM_000100422_612GNAAXX:7:9:10358:4089#0/2_CONS_SUB_SUB_CMP']; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=154; reverse_tag=aattaac; count=1; seq_length=100; mode=alignment; head_quality=66.9; seq_b_single=47; 
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactacaggcaat
agctcaaaactcaaagaacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:33:9900:9541#0/2_CONS_SUB_SUB ali_length=62; seq_ab_match=62; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=13a_F730603; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=247.949713348; seq_a_mismatch=0; forward_tag=aattaac; seq_b_mismatch=0; experiment=wolf_diet; mid_quality=78.8955223881; avg_quality=77.3506493506; seq_a_single=46; score_norm=3.99918892497; merged=['HELIUM_000100422_612GNAAXX:7:33:9900:9541#0/2_CONS_SUB_SUB']; reverse_score=72.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=aattaac; count=1; seq_length=100; status=full; mode=alignment; head_quality=67.0; seq_b_single=46; 
ctagccctaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:115:3625:10051#0/2_CONS_SUB_SUB ali_length=59; seq_ab_match=59; sminR=40.0; tail_quality=93.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=49; sample=13a_F730603; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=235.999642017; seq_a_mismatch=0; forward_tag=aattaac; seq_b_mismatch=0; experiment=wolf_diet; mid_quality=93.0; avg_quality=93.0; seq_a_single=0; score_norm=3.99999393249; merged=['HELIUM_000100422_612GNAAXX:7:115:3625:10051#0/2_CONS_SUB_SUB']; reverse_score=72.0; direction=forward; seq_b_insertion=0; seq_b_deletion=49; seq_a_insertion=0; seq_length_ori=59; reverse_tag=aattaac; count=1; seq_length=5; status=full; mode=alignment; head_quality=93.0; seq_b_single=0; 
caata