# HG changeset patch # User iuc # Date 1492033080 14400 # Node ID aca6a8c9cf830fb85a245d19a73e16eb7098df5e planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/obitools commit e1031e4c94b25d1ed535bf221764ab801b710ab2 diff -r 000000000000 -r aca6a8c9cf83 macros.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/macros.xml Wed Apr 12 17:38:00 2017 -0400 @@ -0,0 +1,136 @@ + + + + + obitools + + + + 1.0.010 + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + 10.1111/1755-0998.12428 + + + + diff -r 000000000000 -r aca6a8c9cf83 obisort.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/obisort.xml Wed Apr 12 17:38:00 2017 -0400 @@ -0,0 +1,67 @@ + + sorts sequence records according to the value of a given attribute, which can be either numeric or alphanumeric + + macros.xml + + + + + + + '$output' + ]]> + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + diff -r 000000000000 -r aca6a8c9cf83 test-data/illuminapairedend.output.fastq --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/illuminapairedend.output.fastq Wed Apr 12 17:38:00 2017 -0400 @@ -0,0 +1,996 @@ +@HELIUM_000100422_612GNAAXX:7:119:14871:19157#0/2_CONS ali_length=62; direction=left; seq_ab_match=47; sminR=40.0; score=115.761290673; seq_a_mismatch=1; seq_b_deletion=1; seq_b_mismatch=13; seq_a_deletion=0; score_norm=1.86711759151; seq_b_insertion=1; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttgagctattgccggtagtactctggcgaatgattttgttataataattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg ++ +dddddddddddbbdaddcddddddcadaddddaadcccWaaddabdx~~|b~~~~cccccccBcBcccBcBcccBccBBcc~}`kXyxu|~Kyr`b~~~~~~b~~~b~d\cdddddddddddddcacddddcdddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:108:5640:3823#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.881364714; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=2; seq_a_deletion=0; score_norm=3.74002201152; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaacaattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg ++ +ddddddddddddddddbdddddbddddddddddddddddddddddd~~~~~~~~~~~~~~~~~~}~~~~~~~~~~~~~~~~~~~~~~~~~~~Z~~~~~~~~~~~~~b~d]dddddddcdddddcddddddddddddddddddddddcddddddd +@HELIUM_000100422_612GNAAXX:7:97:14311:19299#0/2_CONS ali_length=62; direction=left; seq_ab_match=52; sminR=40.0; score=167.91734192; seq_a_mismatch=5; seq_b_deletion=0; seq_b_mismatch=5; seq_a_deletion=0; score_norm=2.70834422451; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctcttgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg ++ +ddadddddcd`dcddddddddd^dd]ddddddZad`\`]Lcca``YT|~~^||v|z|~ts~~~|r~sZ^~~`~~~~~~~~~~~~~~~~V~b~]~~~~b~~~~~~~~`~b\aadddd`dcdddddc`dddccddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:22:8540:14708#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; score=223.982011431; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=3; seq_a_deletion=0; score_norm=3.61261308759; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgcctcctttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctaaggaggcgg ++ +dddedddddddddedddddddddedddaddbddddddddddddedd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~b~~~~~~~~b~~~~ddddddddddabbb^bdddddddddddcddddddddcddddddddd +@HELIUM_000100422_612GNAAXX:7:57:18459:16145#0/2_CONS ali_length=63; direction=left; seq_ab_match=60; sminR=40.0; score=227.883189945; seq_a_mismatch=3; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.61719349119; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +ddcdddddddddddddddeddddddddddddddddddddcddddd~b~~~~~~b~~~~~~~~~~~~~~c~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:89:10281:5930#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.893859399; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.7402235387; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctgttgccgctagtactctggtgaacaattttgtttgtgtaattatttgtgtttagggctaagcatagtggggtatctaaaggaggcgg ++ +ddddddddddddddddddcddddddddddddd\ddeddecddddddb~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddcddddddcdddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:29:15520:18035#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; score=239.965360923; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.87040904714; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgcctcctttagataccccactatgcttagccctaaacacaagtaattattataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctaaggaggcgg ++ +cccccccc_cc_cccacacb]Y]_`a^__`_Y_`_a_`cc_ccccc~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~dddddddddddddddddddddddddddadddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:6:9274:14951#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; score=223.791171196; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=2; seq_a_deletion=0; score_norm=3.60953501929; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg ++ +dcdddddddddddedddddddddcddddddcddddddddddddddd~~~~~~~~~~~~~~~~~~|z~}a|~~~~}}}}~~~~~~~~~~}~}b~b~~~~~~~~~~~~~~ddddddcdcddcd^ddddddddddddddddddddddeddddddddd +@HELIUM_000100422_612GNAAXX:7:111:2893:6772#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; score=239.959941063; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.87032163005; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgcctcctttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctaaggaggcgg ++ +dddddddddddddddddddddddddddddddddddedddddded_d~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~x~dddddddcdddcdcdddadddddddddddedddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:89:10621:20317#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; score=223.827654468; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=3; seq_a_deletion=0; score_norm=3.61012345916; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgcctcctttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctaaggaggcgg ++ +ddddddddddddddbdddddadddddddd`ddddadadddddddRc~}~~z~~~~~~~t~sw}~~g}~~~~~~~~~~~~~~~~~~~~~~~~~~~~^~b`~~~~~~~~~ddddddddbdbdd_dddadddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:50:2791:11138#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.976017167; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.74154866398; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgcctcctttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctaaggaggcgg ++ +dddddcdddddcddddcd^dddddddddbdbdddddddc\ddddbd~~~~~~~~~~~~~~~~~~b~~~^~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddbdddddddddcddddddddcddddddddddddd +@HELIUM_000100422_612GNAAXX:7:108:17701:18887#0/2_CONS ali_length=62; direction=left; seq_ab_match=58; sminR=40.0; score=215.828321765; seq_a_mismatch=4; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.48110196396; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg ++ +dd[dddddddd``ddddddddddddeddddddcdddddd\ddddddb~~~b~~~~~~~~~~~b~~~~~b~~~{~~{z|{|~~~~~~~~~~~ssx~~~~~~~~~~~~~~c^cddddddddddd`cedddddddddddddddddddddddddddcd +@HELIUM_000100422_612GNAAXX:7:97:10414:9929#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; score=239.864541102; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.86878292101; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg ++ +ddddddddddddddddddddddddddddddddcddddddcdddddd~~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddeddddddddddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:97:9091:15775#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; score=223.861916171; seq_a_mismatch=3; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.61067606728; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg ++ +ddddddddddddddddddddddddddddddddcddddddddddddd~~~~b~~^~~~~~~b~~~~~~~|~~~~~~~~~x~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddddadcdddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:74:8683:20738#0/2_CONS ali_length=63; direction=left; seq_ab_match=62; sminR=40.0; score=243.961428864; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.87240363276; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +tcgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +ddddddddddddddddddddddddddddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~`~~~~~~~~~~~~~~ddedddddddddddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:24:16230:12703#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; score=223.86908915; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.61079176049; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgaagtagtagaacaggctcctctagaaggggataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg ++ +ddd`ddddad_ddaddddddddaddddddc`bO``ddd_a`ddd`a}}~sb{~~~~~~~~~~~~~~~~a~~~~~~y~u~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~b^ddcdbcddadddd_ddbdcdcdddd`ddddbddddddddddddd +@HELIUM_000100422_612GNAAXX:7:4:4214:9434#0/2_CONS ali_length=62; direction=left; seq_ab_match=58; sminR=40.0; score=215.812703412; seq_a_mismatch=3; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.48085005502; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactacaggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg ++ +ddddddddSddddbbdddddddbddabdddbdddddcddddddddd~^~~~{~{~~~~~}~~{~~ym~L||~~wuy~zyxPzjyyvxku~~~~~~~~~~~~~~~~~xbacdddddaddac_^`_`dcddddacddcddddadaa`[``^c`acc +@HELIUM_000100422_612GNAAXX:7:12:17693:3451#0/2_CONS ali_length=62; direction=left; seq_ab_match=62; sminR=40.0; score=247.862243; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.9977781129; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg ++ +dddddddddddddddddddddddddbddddddaddddddcdddddc~~~~~~~~~~~~~~~~~~~~~~~~~}~{~}{v}~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddeddddddddddcdeddddddcddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:3:14624:12375#0/2_CONS ali_length=63; direction=left; seq_ab_match=61; sminR=40.0; score=235.922125108; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.74479563663; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +ddddddddddddddddddcdcddddcddcdcdddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~Y`~~~~~~~~~~~~~~~~~~~~~~~dddddcdddddddcedddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:18:5317:11571#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; score=239.862078449; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.86874320079; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg ++ +ddddddddddddddddddddddddddddddddadddddddcddddd~~~~b~~~~~~~~~~{~~~~~~~~~~~~~~~~~y~~~~~~~~~~~~~~~~~~~~~~~~~~~~edddeddddddddddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:109:17335:11429#0/2_CONS ali_length=63; direction=left; seq_ab_match=56; sminR=40.0; score=195.716358124; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=6; seq_a_deletion=0; score_norm=3.10660885911; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +ddddcdddddcdddddda`ddd^dd`^^`bdd`dR\]``]cdddd~x}~~W}}~~}~~~~~x~s~~z~ccccccccccccccccc~[|~`\~}``~|~~~~~~~~~b~dbdcddddddddadcdddcdcdddcddddaddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:7:10646:8487#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.85443709; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.739587695; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg ++ +dddddddddddddddddddddddddddcdddddddddddddddddd~~~~~~~~~~~~~~~~b~~~~~~~hpoppitzv{~~~~~~~~~~~~~~~~~~~~~~~~~~~cedddddddddddddddccdddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:19:4865:20715#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.923942025; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.74070874234; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg ++ +ddddddddddddddddddddddddddddcdddaddcddddddddddby~~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~bddddddddddddddbcdddddeddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:13:6954:13039#0/2_CONS ali_length=63; direction=left; seq_ab_match=62; sminR=40.0; score=243.872474161; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.87099165336; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatctagaacaggctcctctagagggatataaagcaccgccaagtcctttgagttttaagctattgctagtagtcctctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +dddddddddddddddddddddddddddddddcddddddddddddd~~~~~b~~~~~~~~~~~~~~~~~~~~~~~r~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddcdddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:120:14963:1929#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; score=223.810433242; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.60984569745; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg ++ +ddddddddddddddddddddddddddddddddcddddddddddddd~~~~_~~~~~~~~~~~~~~}~~~}}`z}~~~}~~}~~|~~u~~~~~~~~~~~~~~~~}~~b~dbdddddedddddddadddcddddcddddddddddddddddddddd 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+@HELIUM_000100422_612GNAAXX:7:118:13803:15594#0/2_CONS ali_length=63; direction=left; seq_ab_match=60; sminR=40.0; score=227.87720055; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.61709842144; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +ddddddddddddddddddddddddddddddddddddddddddddd~~~~~b~~b~~~~~~~~~~v~~~~~~~~~~y~{~~~~~~~~~~~a~~~~~~~~~~~~~~~~~~dddedddddbdddd^ddeddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:53:14379:5922#0/2_CONS ali_length=62; direction=left; seq_ab_match=62; sminR=40.0; score=247.982071703; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99971083392; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; 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+@HELIUM_000100422_612GNAAXX:7:117:4291:18111#0/2_CONS ali_length=63; direction=left; seq_ab_match=62; sminR=40.0; score=243.911625561; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.87161310415; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +dddddddddddddddddddddddddddddddddddaddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~ddddddddddddddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:71:14800:3222#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.89389474; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.74022410871; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg ++ +ddddddddddddddddddeddddddddeddddcdddddccdddddd~~~~b~~~~~~~~~~~~~~~~~\~~~}~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dcddddddddeedddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:11:8547:18238#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; score=251.984413754; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99975259927; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +ddddddddddddddddddddddddddddbdcdddddddddcdddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddddddddddddddddddddeddddddddddddd +@HELIUM_000100422_612GNAAXX:7:117:2416:10342#0/2_CONS ali_length=63; direction=left; seq_ab_match=61; sminR=40.0; score=235.921215944; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.74478120546; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +dddddddddddddddcdcddddaddcddddddddbddddbddddd~b~~|b~~~~~}~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddcdcddddY[ZYUd^dbdbdddddddddddddcdddddddddd +@HELIUM_000100422_612GNAAXX:7:78:7151:19928#0/2_CONS ali_length=63; direction=left; seq_ab_match=56; sminR=40.0; score=195.576462373; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=7; seq_a_deletion=0; score_norm=3.10438829164; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacgagaatgttcgccagtgtacttctagcaacaggctgaaactcaaaggacttggcggtggtttacatccctctagaggagcctgttctagatattcgg ++ +ZYQOYVVXRYYOYZZ^^^_bZZ]Z[S[WSR]YbbbDDDDDDDDDDcccccccccccBBcccccccccccccBcccccBccccccccccBccccccccccBccccc~`~WNS]Y_KTUVZ[O_SHW]ZQ_^^^ab`Obb\TY\MPPPPQQQQQQ +@HELIUM_000100422_612GNAAXX:7:71:18821:20471#0/2_CONS ali_length=62; direction=left; seq_ab_match=53; sminR=40.0; score=175.889514148; seq_a_mismatch=7; seq_b_deletion=0; seq_b_mismatch=2; seq_a_deletion=0; score_norm=2.83692764755; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg ++ +ddddddddddddddddddddddddddddd\ddddddddd^ddd`dd~~~~Xx~~~~~{|q}}x}^Y`J`~Xv{wt~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~sbddddddddddddd^bdccddbdddddd`ddddddddddddddcddd +@HELIUM_000100422_612GNAAXX:7:58:15837:8531#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; score=251.96128051; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99938540492; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +ddddddddddddddddddddddddddddddbdddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddcdddddddddddddcdddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:38:10813:16867#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; score=251.97777927; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99964729; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgtttgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +dddddddddddddddddddddddcdddddddddddeddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dbcdddddadadcdddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:68:19115:18847#0/2_CONS ali_length=62; direction=left; seq_ab_match=54; sminR=40.0; score=183.856571062; seq_a_mismatch=6; seq_b_deletion=0; seq_b_mismatch=2; seq_a_deletion=0; score_norm=2.96542856552; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg ++ +cdbdddddddcdbcddc[Tabcddd_`bddaaabd^bb^P`ccd`db{~~b~~~~~~\\~~x~~~~s~~s~~~_~~{~~~~~~~b{~~}~~~~~~~~~~~b~~~~~`~ddd^dd_ddcdd_ddceddedcdddddcddacdddddddddddcad +@HELIUM_000100422_612GNAAXX:7:14:3978:11028#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.869517243; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.73983092328; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgcaggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg ++ +ddcddddddcdddeddddddddcddddddcb`Zbbddddddddddd_~~~~~~~~~~~~~~~~~~~v~~~y~J|~u{|w~~~~~~~~~~~~~~~~~~~~~~~~~~~~~da`dddeddddcdbdaeddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:34:18733:3827#0/2_CONS ali_length=63; direction=left; seq_ab_match=62; sminR=40.0; score=243.983236898; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.87274979204; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +ddddddddddddddddddddddddddddddddddddddddddddd~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:65:6787:8456#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.869006954; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.7398226928; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg ++ +ddddddddbddddeddddedddddddddddddddddddddddddddb~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~d_ddeddddddcdddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:111:7441:14688#0/2_CONS ali_length=63; direction=left; seq_ab_match=62; sminR=40.0; score=243.832560428; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.87035810203; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +ddddddddddddddddddddddddddddddddddddddddddddd~~~~~b~~~~~u~~~~~~~~~~~~~~~~~~}~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddedddddddddadddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:86:10092:15563#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; score=223.927744132; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.61173780858; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgcctcctttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctaaggaggcgg ++ +dddd`dddddbdddddddddddcddddddbdddddccdddddaddd~b~~~~~~~~~~~~~_~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~|bddddddddddcddcddd`^dddddddddddcddddddddddddbdd +@HELIUM_000100422_612GNAAXX:7:16:4418:5128#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; score=223.862543287; seq_a_mismatch=3; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.61068618206; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg ++ +dddddddddddddddddddddddddddddddddddddddbddedddb~~~b~~b~~~~~~~~~~~~~~~{~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~bddddddddddddddedddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:13:5742:9859#0/2_CONS ali_length=58; direction=right; seq_ab_match=55; sminR=40.0; score=207.846895043; seq_a_mismatch=3; seq_b_deletion=50; seq_b_mismatch=0; seq_a_deletion=50; score_norm=3.58356715591; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=0; seq_b_single=0; +ccgcctcctttagataccccactatgcttttctagaggagcctgttctaaggaggcgg ++ +~~~~~~Z~~~~~~~~~b~~~~~~~~~_~~~~~~~~~~}~~~~~~~~~~~~~~~~~~~~ +@HELIUM_000100422_612GNAAXX:7:85:18986:17662#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.874929984; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=2; seq_a_deletion=0; score_norm=3.73991822554; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgaagtagttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctactacttcgg ++ +ddcdddddddddeccdddddddddcbdddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~}~~s~~~~~b~~~~~~b~~~~~~~ddcddddddcddddddddddddd`dddddddddddddddddddded +@HELIUM_000100422_612GNAAXX:7:16:16357:19948#0/2_CONS ali_length=63; direction=left; seq_ab_match=57; sminR=40.0; score=203.860719115; seq_a_mismatch=5; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.23588443039; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcacaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +baNdQdd`dd`da_ddbadbdddcddddd_dbddbdaddbddddd~`~~b~~~~~~b~~{~~~}x}}~}`twyhw~~~~}w|~|~~~~~X~~~|~~~~~Y~~~~~~~~XVRSQ[Qadd``^P^adYdddddddcc`^_dccddcdccdddddd +@HELIUM_000100422_612GNAAXX:7:107:3570:18147#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.924768153; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.74072206698; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttatgctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg ++ +ddada`dcddddddddddddddadd_^b_cdd]dddddcdd_dcdd`~s~a~~~~~~~~}~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~d\d\dddddcdddddadddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:82:8836:11354#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.781218203; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.73840674522; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg ++ +dddddddddddddddddddddddddddaddddcdddddcaddddddb~~~b~~~~~~~~~~~~~~~~~~~~~|~~k{{w~~~~~~~~~~~~~~~~~~~~~~{~~~~~~ddddddddddddddddeddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:41:2354:1449#0/2_CONS ali_length=63; direction=left; seq_ab_match=55; sminR=40.0; score=187.777927438; seq_a_mismatch=5; seq_b_deletion=0; seq_b_mismatch=3; seq_a_deletion=0; score_norm=2.98060202283; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +ddbdddccddddddddddddddddddddaaddddddcddbcdddd~b}bb~b~~~b~q~~}~}~x~x~t~s~|k|~w~~z~~{|{~ccBc~~}~~~~~~a~~a~}p~~baceddbbedddda_aa_c[cdadddddddddddddddadddadd +@HELIUM_000100422_612GNAAXX:7:114:4511:17596#0/2_CONS ali_length=62; direction=left; seq_ab_match=57; sminR=40.0; score=207.883730153; seq_a_mismatch=4; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.35296338956; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgcctccttagaacaggctcctctagaagcgtataaagcaccgccaagtcctttgagttttaagctattgccggtagcactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg ++ +ddeedecddddddbdddcdeeddddPQPPPKKPQPPPPPQdddddd~~~~b~~~~~~~~~~~~~~~~~as~a~~~~~}W~~~~~~~~~~~~~X~~~~~~~~~~~~~~~d^d^dddddddddddddddddddddddbdddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:1:8849:9880#0/2_PairEnd sminR=40.0; pairend_limit=108; ali_dir=left; score=25.9064763088; mode=joined; sminL=40.0; +ggacgggagagggggggggggggggaaggggggggttttggggggggggggccgttttaaaaaaaaaaaaaaaacaacgtgacatggcacagccgtcacaactgtcagctgacgaccactggcagtgagtctactctcctattttgttttttttttcaagcagaagacggcatacgatatcggtctcggcattcctgctgaaccgctcttccgatc ++ +BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB_`X`b[`T[ZWHZTWU]]b]]TZ^__\^`\OOOOO^`^Z^_ba_bbbbbb +@HELIUM_000100422_612GNAAXX:7:94:12367:17729#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; score=223.888596169; seq_a_mismatch=3; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.61110638983; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg ++ +dddddddddddddddddddddddddddcceddccdadbd`dddddcb~~~b}~~~~~~~~~~~~~~~~~x||t~|r~~~~~~~~b~~~~~~~~~~~~~~~~~~~~~~~daddedddddddddd`dddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:30:5374:18213#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; score=251.958026467; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99933375344; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +dddddddddddddeddddddddddddddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:49:11740:5547#0/2_CONS ali_length=63; direction=left; seq_ab_match=62; sminR=40.0; score=243.959827817; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.87237821931; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +ddaddcdddddddedddddddddddddeddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~cddddddddcdddddddddddddddddddddedddddddddd`dd +@HELIUM_000100422_612GNAAXX:7:29:3531:8314#0/2_CONS ali_length=63; direction=left; seq_ab_match=61; sminR=40.0; score=235.837856208; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.74345803504; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +dddddddddddddcddddddddddddddddddcddddddddddcd~a~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~}~~~~~~~~~~~~~~~~~~~~~ddceddddddddddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:114:10615:11217#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.702876359; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.73714316708; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgcctcctttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctaaggaggcgg ++ +ddcdddcddcd`d__^dddddd`cddddddddaaa``a_adddd^d~b~~z~~~~~~~~~~~~~cBcccccccccccccc~y~~s~~~~~~~|~~~~~|~~~~sz~~~adddddd[dad`VRW]VZdddddddddddddc``_ccddddddddd +@HELIUM_000100422_612GNAAXX:7:108:2729:8600#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; score=223.856778095; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.61059319508; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg ++ +dddddddddddddeddddddddcddddddddddWddddcddddddd~~~~b~~~~~}~~~~~~~~~~~`~~~~~~~~~~~~~~~~~~~~~~~a~~~~~~~~~~~~~~~dacdcdeddddddddddddddedddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:103:8847:6326#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; score=251.968264217; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99949625741; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +ddddddddddddddddddddddddddddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddcddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:70:11798:2668#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; score=239.988667111; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.8707849534; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgaagtagttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacctttctagaggagcctgttctactacttcgg ++ +dddddddddddddddddddddddddddddddddddddddddddddd~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dcdddddcddddddddbddedddddddcdeddeddddddddddddd +@HELIUM_000100422_612GNAAXX:7:4:18261:9148#0/2_CONS ali_length=63; direction=left; seq_ab_match=61; sminR=40.0; score=235.937993338; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.7450475133; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +ddeddddddddddcdddddddddddddeddddddddddddddddd~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~`~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:29:18416:16620#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.873033258; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.7398876332; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgcctccttagaacaggctcctctagcagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagaactctggcgaataattttgttataataattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg ++ +ddddddddddddddddccddddddd`d\dd`bH[`bbb^bdddddd~~~~~~~~~~~~~~~~~~~~~~~~~`~s~u||Fz~~~~~~~~~~~~~~~~~~~~~~~~~~~~d`ddcdbdcddbbbb\dadddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:32:18262:6966#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; score=239.975704028; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.87057587142; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg ++ +edddddddddddddddddddddddddddddddddcddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~`~~~~~~~~~~~~ddddddddddbdddddddddddddcdddddddbddcdcddcdddbb +@HELIUM_000100422_612GNAAXX:7:110:17877:11976#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; score=223.833250149; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.61021371209; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg ++ +ddddddddddddddddddddddddddd_ddddadddddddddddddb~~~b~~~~~~~~~~~~~~~~~~~vqx{w~~~~~~~~~~x~~~~~~~~~~~~~~~~~~~~`~b\d^dddddddddddcdddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:97:9416:9155#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.872797448; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.7398838298; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg ++ +ddddddddddddddddddddddddddddddddeddddddddddaddb~~~~~~~~~~~~~~~b~~~~~~{~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~eddddddddddddddedddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:72:8905:10715#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; score=251.929473647; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99888053408; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +dddddddddddddeddddedddddddddddcdddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddcdddddadddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:48:5296:16375#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; score=251.994265764; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99990898038; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +dddddddddddddcdddd`ddddddddcddbdddddaddddbddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddd`dbdaaddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:53:6550:1163#0/2_CONS ali_length=63; direction=left; seq_ab_match=61; sminR=40.0; score=235.996766828; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.74598042584; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgtttgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +dddddddddddddddddddddddedddddaddddddddddddddd~b~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddddddddddddddddddddeddddddddddddd +@HELIUM_000100422_612GNAAXX:7:120:1900:13295#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=219.874676069; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=1; score_norm=3.54636574304; seq_b_insertion=0; seq_a_insertion=1; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgcgcgtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg ++ +dddddddddddddcdddddddddddddddddddcdddddcdddddd~~~~b~~~~~~~~~~~~~~~~~~~opfFhtpp~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dcddddcdddcdddddcdddddddddddddddeddddedddddddd +@HELIUM_000100422_612GNAAXX:7:96:19317:7551#0/2_CONS ali_length=62; direction=left; seq_ab_match=58; sminR=40.0; score=215.85731879; seq_a_mismatch=3; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.48156965791; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg ++ +ccccccbccccccccccccccc^cccccccbcXcbccccccccccc~q~|a~~~~~~|~~~~az~~~|_snppphy~~xq{tzz~~~~~~~~Z~~~~~~~~~~~~~~~d_ddddddddcddd`bbdddddddcddddddddddddddddddddc +@HELIUM_000100422_612GNAAXX:7:93:18556:10479#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; score=223.913795643; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.61151283295; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgcctcctttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctaaggaggcgg ++ +ddddddddcddcddcddddddddddddddddddddddddddddddd~b~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~dddddddddddddbdddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:44:19605:15509#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; score=223.916231984; seq_a_mismatch=3; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.61155212878; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg ++ +dddddddddddddddddddddddddbddddddcddcdcdacddcdd~r~~`~k`~~p~~~_~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~z}y~~~~~~~~~~~~~~a_d_dddddddddddcdadddddddcdddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:40:3610:8896#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; score=239.967241748; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.87043938304; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgaagtagttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctactacttcgg ++ +dddddddddddddddddddddddddddddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~~~dddddddddddddedddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:41:19763:3556#0/2_CONS ali_length=62; direction=left; seq_ab_match=62; sminR=40.0; score=247.825851895; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99719115959; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg ++ +ddddddddddddddddddddddddddddddddcddddddddddddd{~~~~~~~~~~~~~~~~~~~v~~~~~~~~jz{m{~~~~~~~~~~~~~~~~~~~~~~~~~~~~dacddddddddddddcddddddddddddddededddddddddddWd +@HELIUM_000100422_612GNAAXX:7:111:7660:7465#0/2_CONS ali_length=63; direction=left; seq_ab_match=58; sminR=40.0; score=211.768626837; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=3; seq_a_deletion=0; score_norm=3.36140677518; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +dbdbddcdddddddddd^dd`dddddcddcdddddd`ddddddcd~~~~~~~~_~~~~~~~~~~~~~Ky~~~~~~c~Y}~~s~~`~x~}~}~{~Z|h~~~~~~~~~~~dYYdddaddaaddad^dddcddddddcdddddddadcdddd``dd +@HELIUM_000100422_612GNAAXX:7:76:10822:18722#0/2_CONS ali_length=63; direction=left; seq_ab_match=58; sminR=40.0; score=211.83390837; seq_a_mismatch=5; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.36244299; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacacgaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +ddddddbcdbdddddddd_dddddddcacbdcddddTbdcddddd~`~~~z~~~~~~S~~~~~~b~~`~~~t~t~~}~~~~~~~~~~~T~~~|~~~~~~~~~~~~x~~ddddadddd_ddbdcbdddadddcdddddddbdddddddbddddd +@HELIUM_000100422_612GNAAXX:7:76:8491:8900#0/2_CONS ali_length=62; direction=left; seq_ab_match=57; sminR=40.0; score=207.828655885; seq_a_mismatch=4; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.35207509492; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgcaggtagcactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg ++ +dcddddddddddddadddddddadddcaddddadcdddddddddddb~~~b~~~~~o~~~~~~~~~~~~~z}F}~xq~N}~~y~~~}~~~~wu~~~~~~~~~~~~~^~b[bcc_ccccbcccccccbcbabacccccccbc_ccbbb`bccccc +@HELIUM_000100422_612GNAAXX:7:47:7619:9866#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; score=239.867362445; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.86882842653; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg ++ +dddddeddddddedddddddddeddddddddddddddddddddddda~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dedddddddddddddddddddddddddddddddddddddddedddd +@HELIUM_000100422_612GNAAXX:7:1:8649:5297#0/2_CONS ali_length=104; direction=right; seq_ab_match=104; sminR=40.0; score=415.823014771; seq_a_mismatch=0; seq_b_deletion=4; seq_b_mismatch=0; seq_a_deletion=4; score_norm=3.99829821895; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=0; seq_b_single=0; +ccagcgagatcccattgagtctctgcacctatcctttttttcgttttctgaatctttgtttggaaaaaaacaggatttggctcaggattgcccgatctcgctgg ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@HELIUM_000100422_612GNAAXX:7:99:14742:5591#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.982488051; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.74165303308; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgcctcctttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctaaggaggcgg ++ +dddddddddddddddddeddddddddddddddddddddddddddcd~b~~~~~~~~~~~~~a~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddddcddddddddddcdddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:95:3274:5435#0/2_CONS ali_length=63; direction=left; seq_ab_match=61; sminR=40.0; score=235.944669323; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.74515348131; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +dddddddddddcddddddddddccddddddddddcdddddddddd~~~~~b~~~~~~~~~~~~~~~~~~~~~~~~~{~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~ddddcdddddddddcdddddddddddedddddddeddcdcddddd +@HELIUM_000100422_612GNAAXX:7:74:8396:18135#0/2_CONS ali_length=63; direction=left; seq_ab_match=61; sminR=40.0; score=235.858013544; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.74377799276; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +ddddddddddddddddddddddddddddddddddddddddddddd~W~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddcddddddddddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:9:10358:4089#0/2_CONS ali_length=61; direction=left; seq_ab_match=54; sminR=40.0; score=181.620394786; seq_a_mismatch=1; seq_b_deletion=1; seq_b_mismatch=6; seq_a_deletion=0; score_norm=2.97738352108; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=47; +ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagttctttgagttttgagctattgcctgtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg ++ +ddcddddddddbdddddddddd`ddadddddda`]][\[]cdd`ccacccBcccccccccccBccccccccccBccccBcccccccccccccccc~~~ZzzzwX~yz`^dcddcddddddabdad^dddbbcbdddddddbdbbcddddddddd +@HELIUM_000100422_612GNAAXX:7:78:10338:12302#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.881878067; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.74003029141; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg ++ +ddddcddddddddddddddddddddddddddddddddddddddddd~a~~~~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddeddddddddddddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:95:17941:6113#0/2_CONS ali_length=63; direction=left; seq_ab_match=57; sminR=40.0; score=203.869927289; seq_a_mismatch=6; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.23603059189; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +ddedddddddddedddddddddddddddddddddddddddddddd~b~~~b~~b~~~bb~~~~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~acede^cdddc`dd`ddddedadddedddddddddddcdddaacc +@HELIUM_000100422_612GNAAXX:7:59:11971:12063#0/2_CONS ali_length=62; direction=left; seq_ab_match=62; sminR=40.0; score=247.995051728; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99992018915; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgcctcctttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgtcagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctaaggaggcgg ++ +ddddddddddddddddddddeddddddacddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~|~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddcdddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:47:3878:6141#0/2_CONS ali_length=62; direction=left; seq_ab_match=62; sminR=40.0; score=247.947034203; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99914571295; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg ++ +dddddddddddddddddddddddddddddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddddddddddddddddddddcddddddddddddd +@HELIUM_000100422_612GNAAXX:7:21:5487:19582#0/2_CONS ali_length=62; direction=left; seq_ab_match=58; sminR=40.0; score=215.831528178; seq_a_mismatch=3; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.4811536803; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg ++ +dddaddbddddcdcddddddddddddddbddcdddcddddddddd_~v}~~}{~~~~b~}ZZ}}}t~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~d`dbdddbdcdcdacddddddddddddaddddddddddeddddddd +@HELIUM_000100422_612GNAAXX:7:38:13721:2004#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; score=239.856433937; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.86865216027; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg ++ +ddcccd`dddddddddddddddddddbbddbd^ddabddddddddd~{~~~~~~~~~~~~b~~~~~~~~~~~~~~~}}~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~d`ddddddddddddddd]addddbddddddddcddddddddddddd +@HELIUM_000100422_612GNAAXX:7:111:19168:18517#0/2_CONS ali_length=63; direction=left; seq_ab_match=60; sminR=40.0; score=223.825469474; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.55278522975; seq_b_insertion=0; seq_a_insertion=1; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=46; +ccgaagtagttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattatccgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctactacttcgg ++ +ddddddddddcddddddddddddddcdddbddddddbcdddd^dd~~~{~_~~~~~~~~~y{}{~U~{|~~~~~n~~|~~~~}x~~~~~~~~~~~~~~~~~^~~~~~~ccdaddbdddbddb_ddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:89:16162:8889#0/2_CONS ali_length=63; direction=left; seq_ab_match=62; sminR=40.0; score=243.971622168; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.87256543123; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattg ++ +dddddedddeddddddddddddddddddddddddddddddddddd~~c~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddedddddddeddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:74:4888:6953#0/2_CONS ali_length=63; direction=left; seq_ab_match=61; sminR=40.0; score=235.986171514; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=2; seq_a_deletion=0; score_norm=3.74581224625; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +ddddddddddddddddddddddddddddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~]~~~~~~~~~~~~~~~~~~~dddddddddeddddddddddddddddddddcdddddddddddddd +@HELIUM_000100422_612GNAAXX:7:41:16707:19819#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.94731954; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.74108579903; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg ++ +dedddddddddddbddedadddddccddddddaddbddd^a`daddb~~~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~bbdddddddddddddddddddddddddddddddccddddddddddd +@HELIUM_000100422_612GNAAXX:7:7:7916:16505#0/2_CONS ali_length=58; direction=right; seq_ab_match=58; sminR=40.0; score=231.999536374; seq_a_mismatch=0; seq_b_deletion=50; seq_b_mismatch=0; seq_a_deletion=50; score_norm=3.99999200644; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=0; seq_b_single=0; +ccgcctccttagaacaggctcctctagaaaagcatagtggggtatctaaaggaggcgg ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@HELIUM_000100422_612GNAAXX:7:41:9316:2256#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; score=239.993848494; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.87086852409; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgcctcctttagataccccactatgcttagccctaaacacaagaaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctaaggaggcgg ++ +dddddddddddeddddcdddddddddddddddddddddddddddNd~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddcadedddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:67:7582:3642#0/2_CONS ali_length=63; direction=left; seq_ab_match=62; sminR=40.0; score=243.962430842; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.87241953718; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +ddddddddddddddddddddddddddddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~`~~~~~~~~ddddddddddddddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:42:8435:4155#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; score=251.987540492; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99980223003; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +ddddddddeddddddddddddddddddddddddddddd`dddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:1:8702:15835#0/2_CONS ali_length=85; direction=left; seq_ab_match=85; sminR=40.0; score=339.959438284; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99952280334; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=23; seq_b_single=23; +cccatcgctcagatatccgttgccgagagtcgtgtagattatatagaattgcaactcggggtgcggccagcaagccagccacatcccctcgttaggcacagtgttccttgacgccttcggtgagcgatggg ++ +ddddddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~{~~~~~~~~ddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:33:9900:9541#0/2_CONS ali_length=62; direction=left; seq_ab_match=62; sminR=40.0; score=247.949713348; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99918892497; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccaattaacttagataccccactatgcctagccctaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg ++ +dddddddddddddcddddddddddddddddddd\`cccccdddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddedddddddddecddcddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:48:7139:3789#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; score=251.902451987; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99845161885; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +ddddddddddddddddddddddddddddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:46:13269:4134#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; score=251.997344433; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99995784814; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +dddddddddddddeddddddddddddddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddcdddddddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:46:9246:19171#0/2_CONS ali_length=62; direction=left; seq_ab_match=52; sminR=40.0; score=167.802718427; seq_a_mismatch=10; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=2.70649545851; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgcctcctttagataccccactatgcttagccctaaacacaagtaattattataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctaaggaggcgg ++ +ddadddddddcaddddddddbdcdddd`dddddddddd^bddadcd~b~~~~~b~~~~~~~_`~~~~~b~~~~~S~~~~~~~~~~b~~n~`~~k~zoY|~~~~jb~~~DDDDDDDDDDDDDDDDDD`M\^ca__T^^Y^c^c_[`[ddddd`cd +@HELIUM_000100422_612GNAAXX:7:37:12979:6595#0/2_CONS ali_length=58; direction=right; seq_ab_match=58; sminR=40.0; score=231.998796685; seq_a_mismatch=0; seq_b_deletion=50; seq_b_mismatch=0; seq_a_deletion=50; score_norm=3.99997925319; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=0; seq_b_single=0; +ccgcctcctttagataccccactatgcttttctagaggagcctgttctaaggaggcgg ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@HELIUM_000100422_612GNAAXX:7:95:1688:9598#0/2_CONS ali_length=63; direction=left; seq_ab_match=54; sminR=40.0; score=179.847313607; seq_a_mismatch=6; seq_b_deletion=0; seq_b_mismatch=3; seq_a_deletion=0; score_norm=2.8547192636; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtatcactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +aaaddbdbddbd`bdc^`[cddbddd`d_ddddcd^^`[`ddd[d~~~~pW~~`xzxwr~p{vvwxjwItwwrxxm|FPS~~}}}~z|~~__~~~~~~~~~~~~~[~~bbacb`adcdddd`cddddddddbbddddddddddddcdddcddd +@HELIUM_000100422_612GNAAXX:7:55:16528:11418#0/2_CONS ali_length=62; direction=left; seq_ab_match=58; sminR=40.0; score=215.988419267; seq_a_mismatch=4; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.48368418172; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcactagcttacaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattag ++ +ddddbadddddddccddccddddaaadddcdddddddddddcd`da~~~~~~~~~~~~x~~~~~\~~~~~~~~~v~~~~~~~~~~\~~~~~~~[~~~s~~~~~~~~]~cY__]\QPPPP`]`[`c^^[cccccccccccaba_a\``]_WK[T[ +@HELIUM_000100422_612GNAAXX:7:32:18486:7018#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; score=251.909476689; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99856312205; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +dddddedddddddddddddddddddddddddddddddddddedcd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddeddddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:85:2105:21152#0/2_CONS ali_length=63; direction=left; seq_ab_match=57; sminR=40.0; score=203.706262238; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=4; seq_a_deletion=0; score_norm=3.23343273394; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +ddddddddddddddddddddddddccdddddcddedddeddddbd~b~~{~~~~~}~}\~~~~cccccccccccccccccc~~~x~j~~~~~~~~~b~`s~b~~~~b~d`dddaddddddddddaddaddddcdddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:83:6795:14061#0/2_CONS ali_length=62; direction=left; seq_ab_match=62; sminR=40.0; score=247.992382324; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99987713426; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgcctcctttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctaaggaggcgg ++ +dddddddddddddcddddddcdcddddddddddddddddddddd_d~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddcddddddddddbdddbdddddddddcddddeddddddddddddd +@HELIUM_000100422_612GNAAXX:7:73:16525:10764#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.90336822; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.74037690678; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg ++ +ddcddddddddddedddddddddddddadddd[bddddb_dddddd~~~~~~~~~~~~~~b~b~~~~~|~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b^ddddcddcddddddd_cdddddcddbdddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:19:6313:16567#0/2_CONS ali_length=63; direction=left; seq_ab_match=60; sminR=40.0; score=227.826165596; seq_a_mismatch=3; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.61628834279; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagccctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +ddbcddddcdddbdcdcdddddadddcddcdYab`__b_bddcdd~~~~O_~~~~~}~~u}~~~~~~~a|~~~z{mrwvx~r~|w}}~~~~~~~~~~~~~~~~~~~~~dddddcaddddddcddddddeeddddddddebddddddddddddd +@HELIUM_000100422_612GNAAXX:7:114:8865:19510#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; score=239.913762365; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.86957681233; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg ++ +ddddedddddddddddddddddcddddddddddddddddddddddd~a~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddcdddddddddddddddedddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:111:8478:1127#0/2_CONS ali_length=63; direction=left; seq_ab_match=57; sminR=40.0; score=203.794981474; seq_a_mismatch=5; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.23484097578; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgcgttccaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +dddd_ddddcdadddddcddddbdddddcd^dabdddcaddddda~]v~~Z~~`puIwxxH~|~}~~~~~~~|~~y}vp~{~~~~|yz~~~~~~~{~~~~~~|~~~b~ddbMddddddddddadddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:81:15726:2345#0/2_CONS ali_length=63; direction=left; seq_ab_match=56; sminR=40.0; score=195.641193686; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=7; seq_a_deletion=0; score_norm=3.10541577279; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccgagtgctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +````Z]]cbb`ccbcc[`ca`bU[cQZ___cQbc`DDDDDDDDDDcBcccBccccccccccccccBccccccccccBcBccccccBccBc~~~~qp~wws~~{~~s~~b_cc__ba_accb_cXJWXWaabcac_c`[__[[_]_aa__`___ +@HELIUM_000100422_612GNAAXX:7:13:14386:20412#0/2_CONS ali_length=63; direction=left; seq_ab_match=60; sminR=40.0; score=223.918527801; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.55426234604; seq_b_insertion=1; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=45; +ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg ++ +dddddddddddddddddddddddddddcdddddddddddddddddd~~~~b~~~~~~~~~~~~~~~~~~t~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ax~~~~a~~dddddddddddddddcedddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:83:18882:9967#0/2_CONS ali_length=63; direction=left; seq_ab_match=60; sminR=40.0; score=227.922890965; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.6178236661; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +ddddddddddddddddddcdddddddddddddddddddddddddd~b~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~~dddddddddddddddddadddddddddcddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:13:17353:13234#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; score=223.801902424; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=2; seq_a_deletion=0; score_norm=3.60970810361; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgaagtagtagaacaggctcctctagaaaggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg ++ +ddddedddddddddddddddddddcdddddbcUcbdddbddbddadb~~~~~~~~~~~~~~~~~~~~~~~~~~~~~|~~~~~~~~~~~~~]pVo~~~~~x~~~~~}~~b`dWddeddddddddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:53:10153:11845#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; score=239.985596162; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.87073542196; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgaagtagttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctactacttcgg ++ +dddddddddddddcddddddddcddddddddddddddddddddddd~~~~~~~~~~~~~~~a~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddedddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:33:9296:13526#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; score=239.852481933; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.86858841828; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg ++ +ddddadddddddddddddddddcdddddcdddZdddddbddddddd^~~~~~~~~~~~~~~~~~~~~~~~v~~~~~|~|~~~~~~~~~~~~~~~~~~~~~~~~~~~~~d^ddddddddeedddddcdddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:113:2918:18736#0/2_CONS ali_length=62; direction=left; seq_ab_match=58; sminR=40.0; score=215.87931956; seq_a_mismatch=4; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.48192450903; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg ++ +dddddddddddddddddddddddddddddddd_adddddcddddcdb~~~b~~b~~~~~~~~~~~y~~`y~~v~~~v~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~d`_ddddddddddddcdcdddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:63:4725:12502#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; score=239.998975242; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.87095121358; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg ++ +ddadcdddcdddddddddcdddaddadTdd\]R\]ddd``dddddd~~~~~~~~~~~~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~d]dWddddddddeddcdcddddddddddddddddcddddddddddd +@HELIUM_000100422_612GNAAXX:7:7:11110:17924#0/2_CONS ali_length=62; direction=left; seq_ab_match=58; sminR=40.0; score=215.812113201; seq_a_mismatch=3; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.4808405355; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgaagtagttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtcataccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctactacttcgg ++ +ddddcddd_dddddddddddddddcdddcddddddddcdddddd`d~b~~~~~~~~~~~~~~~~~rm{kripoooEJpoolppp~~~~~~~~~~~~~v~~~~~`v~~~dddcdddddddbddddaddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:102:6364:4627#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; score=251.985885517; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99977596059; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +ddddddddddddddddddddddddddddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddcddddddddddcdddddddddddddddddcddddddddddddd +@HELIUM_000100422_612GNAAXX:7:96:8737:4973#0/2_CONS ali_length=58; direction=right; seq_ab_match=57; sminR=40.0; score=223.980140585; seq_a_mismatch=1; seq_b_deletion=50; seq_b_mismatch=0; seq_a_deletion=50; score_norm=3.86172656181; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=0; seq_b_single=0; +ccgcctccttagaacaggctcctctagaaaagcatagtggggtatctaaaggaggcgg ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@HELIUM_000100422_612GNAAXX:7:89:9710:20652#0/2_CONS ali_length=63; direction=left; seq_ab_match=62; sminR=40.0; score=243.910293117; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.87159195424; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttatatccctctagaggagcctgttctagatattcgg ++ +ddddddddddddd`addddddddd`dddad]ccccddcdddcddd~~}~~~~~~~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~}ddadddddddddddddddddddddddadddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:71:17473:7401#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.883938289; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.74006352079; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattaccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg ++ +cccccc^ccccccccccccccccc_cccccabaac^ccccc_cc^c~~~~R~~~~s~~w~~~~~xg~~~~powyw~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~~dddbddbdccddddaddddbdddddddacdddcddddddddddddd +@HELIUM_000100422_612GNAAXX:7:28:9406:18226#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.816671723; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.73897857618; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg ++ +ddddddddddddddddddddddddddddddddcddddddddddedd_~~~b~~~~~~~~~~~~~~x~~~~~~~~~~{~~~~~~~~~~~~~~|~~~~~~~~~~~~~~~~dcedcdcddddddddddddddddddddcddddeddddddddddddd +@HELIUM_000100422_612GNAAXX:7:15:16118:15111#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.917355899; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.7406025145; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgaagtagttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctactacttcgg ++ +dddddddddddddddddddddddddddddddddddddddddd`d]d~~~c~~~~x~~~~~~a~~~w~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddd`dddedddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:58:18618:20959#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; score=223.882241304; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=2; seq_a_deletion=0; score_norm=3.611003892; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg ++ +ddddeddddddddddddddddddddddddddd_ddddddcdddddd~~~}b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~|~~~~~z~~x~~~~~~~~~b~~~~~bddddddddddddddddcddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:46:11654:6037#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; score=239.828425953; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.8682004186; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg ++ +ddddddddddbd`adddddddddddddddddddbcbbb^bcddcccb~~~~~~~~~~~~~~~~}~~~~~~~{~~~v~~z{}~~~~~~~~~~~~~~~~~~~~~~~~~~~dcdddddddddcdddedddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:2:6320:8068#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; score=251.910304163; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99857625655; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +ddedddddddddddddddddddddddddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddeddddbdddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:46:1592:11866#0/2_CONS ali_length=62; direction=left; seq_ab_match=62; sminR=40.0; score=247.993604064; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99989683974; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg ++ +ddcdddcddddbdddcdddd_ddaddddd_dddbcd\dddddd]dd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddcddcddd`dddcddcdddddddddddddcdd`dddddcdd`ddd +@HELIUM_000100422_612GNAAXX:7:119:2660:19129#0/2_CONS ali_length=63; direction=left; seq_ab_match=61; sminR=40.0; score=235.859804076; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=2; seq_a_deletion=0; score_norm=3.74380641391; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +ddddcdddedddedddddddddddddddcddcddddddddcdddd~~~~~~~~~~~~~~~~z}~~~~~~~~~~~~~~~~~~~~~~~~~~~~k~~~~~~~~~~b~^~~~dddbddddddddddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:115:3625:10051#0/2_CONS ali_length=59; direction=right; seq_ab_match=59; sminR=40.0; score=235.999642017; seq_a_mismatch=0; seq_b_deletion=49; seq_b_mismatch=0; seq_a_deletion=49; score_norm=3.99999393249; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=0; seq_b_single=0; +ccaattaacttagataccccactatgccaatactagaggagcctgttctagttaattgg ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@HELIUM_000100422_612GNAAXX:7:100:17253:5513#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.761122002; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.73808261294; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg ++ +dddcddcddccddbddddddddbdcdcdddbdddddcddddddddd~~~~|{|~~~~b~~~~~~~~~~s~~~~~x~~~~~~~rs~zqwxy~z~~|}~z~}|{^v~~~~c``Zc`cXWcc[WYYZccccccc_ccccccccWbcc`cc]cccccc +@HELIUM_000100422_612GNAAXX:7:82:6036:6921#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.901981419; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=2; seq_a_deletion=0; score_norm=3.74035453901; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg ++ +dddddddddddddddddddddddddcd`dddbaddddcdccddddd~{~|~~~~~~~~~~~~~~~~~~~}~~~~~~~~]~~~~~~~~~~~~~~~~~~~~~~~b~~~~~dcddddddddeddddbcddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:120:4726:20808#0/2_CONS ali_length=62; direction=left; seq_ab_match=62; sminR=40.0; score=247.810697755; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99694673798; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg ++ +ddddecdcdddddcedddddddddddddbedddddcdddddddddb~~~~~~~~~~~~~~ty}y~~~~~~~~~~~~{~~~~~~~~~~~~~~~~~~~~~~~~~~~~~r~ddddcdddddcdd`dddddedddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:99:8183:13912#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.903850642; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.74038468777; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctcctagaggagcctgttctagttaattgg ++ +ddddddddddddceeddddddddddddddddddddddcddddddeb~Z~~~~~~~~~~~~~~~~~}~l~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~dadcdedddcdddddddd`ddddddddddddddddcddddeddddd +@HELIUM_000100422_612GNAAXX:7:10:4085:7492#0/2_CONS ali_length=63; direction=left; seq_ab_match=62; sminR=40.0; score=243.95581367; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.87231450269; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +ddddddddddddddddddddddddddddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~ddddddddddddddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:35:12723:18557#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; score=251.945522222; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99913527337; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +ddddddddddddddddd`ddddddddaadddcddddddddddddd~~}~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~cdddcdddddddbddddddcdddddddddddcddddddddddddd +@HELIUM_000100422_612GNAAXX:7:97:10495:17046#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; score=239.938979134; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.86998353443; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg ++ +ddedecdddddddbbdddddddddcddddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~a~~~~~~~~~~~~dddddddddddddddcddddddddddedddddcddddddddcdddd +@HELIUM_000100422_612GNAAXX:7:73:17068:16620#0/2_CONS ali_length=58; direction=right; seq_ab_match=57; sminR=40.0; score=223.962007947; seq_a_mismatch=0; seq_b_deletion=50; seq_b_mismatch=1; seq_a_deletion=50; score_norm=3.86141393012; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=0; seq_b_single=0; +ccgcctccttagaacaggctcctctagaaaagcatagtggggtatctaaaggaggcgg ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~|~ +@HELIUM_000100422_612GNAAXX:7:23:13304:5167#0/2_CONS ali_length=62; direction=left; seq_ab_match=62; sminR=40.0; score=247.997553566; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99996054139; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg ++ +dddddddddddddddddddddddddddddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddcdddddddddddddcdddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:54:2542:19180#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; score=239.868258533; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.86884287957; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg ++ +ddddddddddddddddddddddddddddddddbddddcdccddddd~~~~b~~~~~~~~~~~~~~~~~|~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dadbdddddddddddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:1:8613:9165#0/2_CONS ali_length=101; direction=left; seq_ab_match=99; sminR=40.0; score=387.825112777; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.83985260176; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=7; seq_b_single=7; +ccagagcgagtgggcaatcctgagccaaatctttatttttagaaaaacaaaagggcttcagaaagcaaaaataaacataaaggataggtgcagagactcaatggactcgctctgg ++ +ddddddd~~~~b~~~~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddd +@HELIUM_000100422_612GNAAXX:7:109:17917:15159#0/2_CONS ali_length=63; direction=left; seq_ab_match=61; sminR=40.0; score=235.857230348; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.74376556108; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +dddedddddddddddddddddddddddddddcddddddddddddc~~~~~b~~a~~~~~~~~~~~~~~~~~~~~~}~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddcdddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:59:18371:8149#0/2_CONS ali_length=62; direction=left; seq_ab_match=62; sminR=40.0; score=247.998742319; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99997971482; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg ++ +ddddddddddddddddddddddddddddcddddddddddddddddc~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddedddddddddddddddddcdddddddddddddd +@HELIUM_000100422_612GNAAXX:7:1:8670:8750#0/2_CONS ali_length=90; direction=right; seq_ab_match=90; sminR=40.0; score=359.914979278; seq_a_mismatch=0; seq_b_deletion=18; seq_b_mismatch=0; seq_a_deletion=18; score_norm=3.99905532531; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=0; seq_b_single=0; +ccgaggcttactaggattagataccctattatttttgaatgttaatgtttgtttgcttgagtagtattagttatgttcttgaaagcctcg ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@HELIUM_000100422_612GNAAXX:7:86:11013:15234#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; score=239.990009212; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.8708066002; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg ++ +dddddddddddddddddddddddddddddddddddddddddddddd~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:77:18518:10758#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; score=223.70605163; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.60816212307; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg ++ +aaa]`_ccca_a`ccccccccc]c_c_caccaccccccccccc_cc~~~~~~~~~~~~wzz{~~a~~~~y~_~~~~~~~~~~m~sygwqv~ccBccccccccccccc~bb^^bc`U\\`\]XPZ\__[\Z`ZX`accccc``c`Rb`Z`bbccc +@HELIUM_000100422_612GNAAXX:7:63:18851:7688#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; score=223.854412144; seq_a_mismatch=3; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.61055503459; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg ++ +ddddddddddddddddddedddddddddddddddeddddddddddd~~~~b~~~~~~~~~b~a~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddeddddddddddcdddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:22:1798:3790#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; score=223.82253574; seq_a_mismatch=3; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.61004089903; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagcactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg ++ +ddddddddddddcdddddddddddddddddddadddddddddddddb~~~b~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddbddbdadddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:115:14354:10118#0/2_CONS ali_length=63; direction=left; seq_ab_match=59; sminR=40.0; score=219.850378027; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=2; seq_a_deletion=0; score_norm=3.4896885401; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgcgagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +ddddddddddddddddddddddcdddddddddccddcdddddddd~~~~~b~~~~~~~~~~~~~}~~~~~~}Jt~y|w~~~~~~~~~~~~~~~_~~~~~~~~~~~~b~dcddddeddddddddeddcdddddddddddddddddddddddddd 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+ccgaagtagttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctactacttcgg ++ +dddddddddddddddddddddddddddddcdddddddddddddd`d~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~}~dddddddddddddddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:29:12308:10119#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; score=251.866768717; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99788521773; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +ddddddddddddddddddddddddddddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddcdeddddedddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:25:11691:21134#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; score=223.86269299; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.61068859661; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg ++ +dddddddddddddddddddddd`ddcddddddadcdddaddddddd_~~~b~~}~~~~~~~~~~~~~~~}~}~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~d]daddddddcdddddcdddaddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:111:15484:19248#0/2_CONS ali_length=63; direction=left; seq_ab_match=60; sminR=40.0; score=227.828264164; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.6163216534; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +dddddddddddddddddddddddddddddddcdd`dddddddddd~~~~~b~~~~~~~~s~~~~~~~~`{~~}~xy}~{~~~z~{txvzj~~`~~~~~~~~~~~~~~~dddaddddddddddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:103:3594:15996#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.92194325; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.74067650404; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacacaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg ++ +dddddedddddddddddddddddcddddddddddddddddddddddb~~~~~~~~~~~R~}~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddaddddddddddddd\bddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:1:8732:6209#0/2_PairEnd sminR=40.0; pairend_limit=108; ali_dir=right; score=15.9727840143; mode=joined; sminL=40.0; +gctctacagaggcgggggggggggagaaggggaaatcatggggggggggggcgacatctaaaaacatacatacaacccacacctcctaccacacagctcccagcgcaaagatagatcatgatggtaagtagaggcaaatgagatcatctatttttttcaaagagaagacggcatacgatatcggtctcggcattcctgctaaccgctcttccgatc ++ +BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBa[]aa_aaaa]aa`]a\[aZ`\ZcMWR]Z````` +@HELIUM_000100422_612GNAAXX:7:1:14576:14181#0/2_CONS ali_length=63; direction=left; seq_ab_match=60; sminR=40.0; score=223.962708229; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.55496362269; seq_b_insertion=0; seq_a_insertion=1; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=46; 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+@HELIUM_000100422_612GNAAXX:7:43:7980:7908#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; score=239.984831014; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.87072308087; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg ++ +ddddcdddddddddddd`ddddddddbdaddcdddd_cddcdddda~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~`~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~d`ddddddddddccbdddddddddddddcdddeddddddddddddd +@HELIUM_000100422_612GNAAXX:7:105:14135:6905#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; score=239.985379811; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.87073193244; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgcctcctttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcaccagagtactagcggcaacagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctaaggaggcgg ++ +ddddddddddcddedddddddddddddddddddddddddddddddd~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddeddddddddddddbdddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:89:16663:15081#0/2_CONS ali_length=62; direction=left; seq_ab_match=62; sminR=40.0; score=247.939062327; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.9990171343; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg ++ +dddedddddddddddddddddddddddddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddecddddddddddddcdddddcddddddddddddd 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+ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +dddcddddddddcddddddddddddddddddddeddedddddadd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddedddddddddedddddddddddddddddddddddddddded +@HELIUM_000100422_612GNAAXX:7:14:15397:17819#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; score=223.847163266; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.61043811719; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctgttgccgctagtactctggtgaacaattttgtttgtgtaattatttgtgtttagggctaagcatagtggggtatctaaaggaggcgg ++ +ddcddddddddddddddddddddddbddddddcddddddbcddddd~~~~~~~a~~}~~~~~~~~v~~~s~a~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~`~cddddddddddddddddddddddddddddddddddddddddddddd 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+ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg ++ +dddddddddaddddddddddddddcccdddddbdddddabdddbdd~~~~~~~~~~~~~~~~~~~~~~~v~~~~~~~~~~}~~~~~~~~~~~~~~~~~~~~~~~~~~~dadddddddddddddcdddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:42:19757:3800#0/2_CONS ali_length=62; direction=left; seq_ab_match=57; sminR=40.0; score=187.78301321; seq_a_mismatch=1; seq_b_deletion=1; seq_b_mismatch=3; seq_a_deletion=1; score_norm=3.02875827758; seq_b_insertion=0; seq_a_insertion=1; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=46; +ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgtatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg ++ +dddeddddddddddddddddddddddddcdddaddddd`dddddd~~~~~~]~~~~~~~~~~~~~_x~~~~~~~~~~~zv~~~~~~~~~~~`~a~~~|~b~~~~~~~ccdcddddddedddeddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:116:14244:17926#0/2_CONS ali_length=63; direction=left; seq_ab_match=55; sminR=40.0; score=187.882758912; seq_a_mismatch=5; seq_b_deletion=0; seq_b_mismatch=3; seq_a_deletion=0; score_norm=2.98226601448; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgcaagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +dddddddddddddddddddddddedddd`dbcccc`dddddeddd~~~~~~_~~~~b~~~~~~^xz{ZRzpooop~~s~~~~~~`~~~~~~~~~~~~~~~~~b~~~b~dddddeddddddddddddddeddddadddddeddddddddddddd +@HELIUM_000100422_612GNAAXX:7:91:10071:8960#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; score=251.979171474; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99966938848; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +dddddddddddddddddddddddddddddddddddddddddeddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:61:17484:5085#0/2_CONS ali_length=62; direction=left; seq_ab_match=62; sminR=40.0; score=247.996979391; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.9999512805; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgcctcctttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctaaggaggcgg ++ +ddcdddddddddddddddeddddddddddddedddddddddddd`d~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddccddcdddddddddddddddddbddddddddedcd +@HELIUM_000100422_612GNAAXX:7:18:2721:16829#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; score=251.960802952; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99937782464; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +ddddecdededdedddddddddddddddddeeddddedddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dbddddddddddddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:2:7296:17275#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; score=223.923525795; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=2; seq_a_deletion=0; score_norm=3.61166977089; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgcctcctttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctaaggaggcgg ++ +ddcddcdd_dddddddddddddcdddddbcacbcbd`ddddddd_b~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~vbdddddddddddcc]baddbddddbdddddddddddddcdddddddd +@HELIUM_000100422_612GNAAXX:7:58:8591:17028#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; score=223.788176649; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.60948672015; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg ++ +cc^cccccacabcaaccccccccc_\_[__ccaccccc`ac]caaa~~~~_~~`~wx~z~~~~~xxuz{~~~~~~ppppkzzrz~~x~~~~nyw~~~~~y~~a~~~~~ccccccaccbcccccccb_cccccccc_ccccc^]ccccccccccc +@HELIUM_000100422_612GNAAXX:7:13:3469:18630#0/2_CONS ali_length=63; direction=left; seq_ab_match=58; sminR=40.0; score=211.840326012; seq_a_mismatch=3; seq_b_deletion=0; seq_b_mismatch=2; seq_a_deletion=0; score_norm=3.36254485733; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +dddddd`ddadddddddcddddcdddd`ddc_a`^dddd_ddddd~b~~~b~~[~~~~~~~~~~~~~~{yy|{xzsz~~~~~~~~~~~~~~~~~~w{VqLyyx~~~~~ddadcdddadPPQQIdddddddddddddddcddddddd_ddcd`d +@HELIUM_000100422_612GNAAXX:7:72:12959:5736#0/2_CONS ali_length=63; direction=left; seq_ab_match=56; sminR=40.0; score=195.664863172; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=7; seq_a_deletion=0; score_norm=3.10579147892; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatctagaacaggctcctctagaggggtgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +__T__WY_b_Sb__X[_\S_bDDDDDDDDDDDDDDDDDDDDDDDDccccccccccccccccccccBcccccccccBBBcc~_~~~~~~bi~~b~~~~~~~~~~~~~~~dddd^dddddbbbbSb\^b^cacdddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:1:8858:2566#0/2_PairEnd sminR=40.0; pairend_limit=108; ali_dir=left; score=15.7312871104; mode=joined; sminL=40.0; +ggctcggagagagggggggggggggggaggggggggatccgggggggggggggtcttacaaaaaaacacaaaacacataaccactctcacacccacactcgcacacccggatgtggttgtggtgtgcgcgtggatggtgtggtgtggtttttttttcaagcagaagacggcatacgagatcggtctcggcattcctgctgaaccgctcttccgatc ++ +BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBa_a_a]aaaa_`Y`_W]Y[S]]aaaabbbbbcbbbbbbbbbbbbb`bbbbbbbbbbbbbb +@HELIUM_000100422_612GNAAXX:7:115:6263:17700#0/2_CONS ali_length=63; direction=left; seq_ab_match=61; sminR=40.0; score=235.853550005; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.74370714293; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +ddddddddddddddddddddddddddddddddddddddddedddd~b~~~b~~~~~~~~~~~~~~~~~~~~~{~~xxy{z|y~~~~~~~~~~~~~~~~~~~~~~~~~~dddedddddddddddcdddddddddddddddcddddddddddddd +@HELIUM_000100422_612GNAAXX:7:7:15538:8325#0/2_CONS ali_length=63; direction=left; seq_ab_match=52; sminR=40.0; score=151.662770749; seq_a_mismatch=0; seq_b_deletion=1; seq_b_mismatch=10; seq_a_deletion=0; score_norm=2.40734556744; seq_b_insertion=1; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatctagaacaggctcctctagagggatgtaaagcaccgcccagttctttgagtttcatgctgttgctagtagtactctggccgacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +__\b\][\XVbZX_\_b[abbDDDDDDDDDDDDDDDDDDDDDDDDcBcccBcccccBccccccBccccccBBccccccccccccccBcca~~~^YgzYxq~~~~}y~~_ccccac_accacba\S_][_cc^cccaccbacba`a_c_`a_aa +@HELIUM_000100422_612GNAAXX:7:120:16005:7955#0/2_CONS ali_length=64; direction=left; seq_ab_match=61; sminR=40.0; score=231.880428082; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=2; seq_a_deletion=0; score_norm=3.62313168878; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=44; seq_b_single=44; +ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +dddddddddddddddddddddddddddddddddddddddcdddd~~~~~~~~~~~~~~~~~~`~~~~~~~{~~~~~~~~~~z~~~~~~~~~~~b~~~~~~~~~b~~~~dddddddddddddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:115:3396:14235#0/2_CONS ali_length=63; direction=left; seq_ab_match=61; sminR=40.0; score=235.745732131; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.74199574811; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +ddddddddddddddddddddddddddddddddcdcdddddddddd~~~~~b~~~~~~~~~~~~~~~~~~~s~~~~~~~{~~~~|~ccc~}~}~~~~~~~~~b~~~~~~dddcdddddadddddcdddddddddddddddeddddddddddddd +@HELIUM_000100422_612GNAAXX:7:32:15197:9940#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.86950825; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.73983077822; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg ++ +dddddedddddddddddddddddddeddddddcddddddcdddd`db~~~~~~b~~~~~~~~~~~~~~~{~~~~~}}tw~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddcddddddddddddacddddddddddddcddddddddddddddcd +@HELIUM_000100422_612GNAAXX:7:7:17828:13825#0/2_CONS ali_length=62; direction=left; seq_ab_match=56; sminR=40.0; score=187.930205125; seq_a_mismatch=2; seq_b_deletion=1; seq_b_mismatch=3; seq_a_deletion=0; score_norm=3.03113234072; seq_b_insertion=1; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgcctcctttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctaaggaggcgg ++ +ddeddcdddddcddddddddddddddddddbddddddddacbaa[b~b~~~~~~~~~~~~~a~~~~~~~~~~~~b~~~~~~~~~~~b~~~~~~~b}~~~~~~~~~~~_ddddddddddddccdddcdddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:53:2128:13141#0/2_CONS ali_length=62; direction=left; seq_ab_match=62; sminR=40.0; score=247.978630411; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99965532921; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgaagtagttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctactacttcgg ++ +ddcddddddddddddddddddddddddddddddddddddddd^dad~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddcddddddddddcdddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:1:8644:15229#0/2_CONS ali_length=106; direction=left; seq_ab_match=103; sminR=40.0; score=399.617128202; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=2; seq_a_deletion=0; score_norm=3.76997290756; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=2; seq_b_single=2; +cacacgctctccattgagtctctgcacctatcctttttgattttcgctttctgaatctttgtttgttttcggaaaacgtgatttggctcaggattgcccagagcgtgtgg ++ +dd~~~~~~~~~~~~~~~~~~~~~~~~{~~~~b~~~~~~~~~{|~~~~s|~v~~|p~~~~~~~~~~~u~qy~a~~~~~~~x~|~~~~z{~|~o|~{yvwwy~~|~~[~~cb +@HELIUM_000100422_612GNAAXX:7:72:7057:20448#0/2_CONS ali_length=62; direction=left; seq_ab_match=62; sminR=40.0; score=247.94833093; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.9991666279; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg ++ +ddddddddddddddddddddddddddddddeddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddebddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:10:9237:10532#0/2_CONS ali_length=62; direction=left; seq_ab_match=57; sminR=40.0; score=207.90944986; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=3; seq_a_deletion=0; score_norm=3.35337822355; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaattattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg ++ +ddcdccddddcddcdcdcdddd[a^[]]V]adadddacdddadddd~~~~b~~b~~~~~~o|~|xpppoj~~Y~~~~~~~~~~~~~~~\~~N~~~~~~~~~~~~~~~~`\_addddddddddd`cSccadddddd`dddddbabdcdddddddd +@HELIUM_000100422_612GNAAXX:7:16:16055:12835#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; score=239.868244499; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.86884265322; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg ++ +ddddddddddddddddddddddddddddddddcddddddddddddd~~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~cdddddddddddcddd`bdddddcdddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:17:16877:9760#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; score=223.899159093; seq_a_mismatch=3; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.61127675956; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgcctcctttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctaaggaggcgg ++ +ddddddddddddddddddddeddddcddddddddddddcddddddd~b~~~~~~~~~~~~~a~~~~~~b~~~{~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddddddddddddddddddddeddddddddddddd +@HELIUM_000100422_612GNAAXX:7:47:8320:8571#0/2_CONS ali_length=63; direction=left; seq_ab_match=61; sminR=40.0; score=235.809300513; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.74300477004; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +dddddddddcdddddddddddd`dd`daddd\ddddddaddddad~b~~~~~~~~~~x~~~~~|~~~~~~~~~~~yuu~~~~~~~~~~~~~~~~~~~~~b~~~~~|~~ddddddddddddddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:6:6617:2744#0/2_CONS ali_length=63; direction=left; seq_ab_match=61; sminR=40.0; score=235.875132744; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.7440497261; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +ddddddddddddcadddddddddddddeddddd`ddddddddcbd~b~~~~b~~~~~~~~~~~~|~~~~~~~~y~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddcbddddeddddddddddeddddddddddddd +@HELIUM_000100422_612GNAAXX:7:12:9609:14740#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.833538451; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.73925062018; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg ++ +dddddddeddddddddddddddddddddddddedddddcdddddddb~~~b~~~~~~~~~~~~~~~~~~}~~~~~}~sz~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddcddddddddddcdddddddddddddddddddddddedddd +@HELIUM_000100422_612GNAAXX:7:21:2093:4412#0/2_CONS ali_length=63; direction=left; seq_ab_match=60; sminR=40.0; score=227.763131817; seq_a_mismatch=3; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.61528780662; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +dddddddddddddddddddddddddddeddddddddddddddddd~b~~~~~~~~~~~~~}~~`~~~~~~~~~~~~~Z{~~|{~~~~{~|~|~~~~~}~~~}|~~}}}DDDDDDDD``U`^\[dddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:18:5240:1587#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; score=223.905608015; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=2; seq_a_deletion=0; score_norm=3.61138077443; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg ++ +dddddddddddddddddddddddddddddddddddddddddddddd~}~~~~~~~~~~~~}~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~bdddddddedddddcdddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:88:15797:19191#0/2_CONS ali_length=62; direction=left; seq_ab_match=58; sminR=40.0; score=215.88300628; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=4; seq_a_deletion=0; score_norm=3.48198397226; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg ++ +ddddddddddddddddddddddddddddddddddddddddddcddc~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~`~~~~b~~b~~~~ddeddddddddddcdddddcddddddddcddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:1:8613:5665#0/2_CONS ali_length=90; direction=right; seq_ab_match=89; sminR=40.0; score=351.879189487; seq_a_mismatch=0; seq_b_deletion=18; seq_b_mismatch=1; seq_a_deletion=18; score_norm=3.90976877208; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=0; seq_b_single=0; +ccttcctggggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaccaggaagg ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~ +@HELIUM_000100422_612GNAAXX:7:47:11864:1343#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; score=251.976310174; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99962397102; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +dddddddddddddddddddddddddddddddddddddddddbdcd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddcdddddddddddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:43:8022:8427#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.869859511; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.73983644372; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg ++ +ddddddddddddddddddddddddddddddddcddddddddddddd~~~~b~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddddcdedddddcdddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:95:11283:2557#0/2_CONS ali_length=58; direction=right; seq_ab_match=57; sminR=40.0; score=223.999078974; seq_a_mismatch=0; seq_b_deletion=50; seq_b_mismatch=1; seq_a_deletion=50; score_norm=3.86205308576; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=0; seq_b_single=0; +ccgcctccttagaacaggctcctctagaaaagcatagtggggtatctaaaggaggcgg ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~a~~~~~~~~~~~~~~~ +@HELIUM_000100422_612GNAAXX:7:67:5288:13403#0/2_CONS ali_length=62; direction=left; seq_ab_match=62; sminR=40.0; score=247.999078967; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99998514464; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgcctcctttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctaaggaggcgg ++ +ddeddddddddddddddddddddddddddddddddddcdddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddedddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:7:11998:4462#0/2_CONS ali_length=63; direction=left; seq_ab_match=59; sminR=40.0; score=219.864332815; seq_a_mismatch=4; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.48991004469; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtagtactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +dddddddddddddddddddddddddddddddddddddddddcddd~b~~~~~~~~~~~~b~~~~~~~b~~~~~~~G~}}~~}}~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddcdccddedddddddcdddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:116:12598:9964#0/2_CONS ali_length=62; direction=left; seq_ab_match=53; sminR=40.0; score=175.843125508; seq_a_mismatch=8; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=2.83617944368; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg ++ +dddddddddddddddddddddddddddddddddddddddeddddddb~~~b~~~~~~~~~~~b~~~~~~{~b~b~~~~~b~~~~b~~~b~~~~~~~~~~~~~~~~~~`adddbdddddddcddddddddccdcedddeddddddddddbcecdd +@HELIUM_000100422_612GNAAXX:7:111:18277:17779#0/2_CONS ali_length=63; direction=left; seq_ab_match=61; sminR=40.0; score=235.877819976; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.74409238057; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctattgctagtagtcctctggcgaattattttgttgtaaaattatctatgtttagggctaagcatagtggggtatctaagatattcgg ++ +dddddddddddddcdddddddddddddddddddddddeddddddd~~~~~b~~~~~~~~~~~~_~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dcdddbddddddddddddeddeddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:85:13257:14594#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.92296471; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.7406929792; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctgttgccgctagtactctggtgaacaattttgtttgtgtaattatttgtgtttagggctaagcatagtggggtatctaaaggaggcgg ++ +dddddddddddeddddddddddddddddddddacdddddaddccdda~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~_~~~~~~~~~~~~~~~~~~~~~~~ddddddddddeddddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:17:11935:17343#0/2_CONS ali_length=63; direction=left; seq_ab_match=62; sminR=40.0; score=243.983570342; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.87275508479; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +ddddddddddddddddddddddddddddddddddddddddddddd~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddddddcddddddddddddcddddddddddddd +@HELIUM_000100422_612GNAAXX:7:38:9654:18700#0/2_CONS ali_length=63; direction=left; seq_ab_match=62; sminR=40.0; score=243.950053136; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.87222306566; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +dddddddddddddddddddddddddddcddddcddddddddddcd~~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddeddddddddddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:101:11720:19906#0/2_CONS ali_length=62; direction=left; seq_ab_match=57; sminR=40.0; score=207.841888471; seq_a_mismatch=4; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.35228852373; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg ++ +dddddddddddddddddddddddddddddddd^ddbbcc^dcdddd_~~~c~~b~~~~x~}~~~~}~~b~}sq~~}t{z~~{~~psh~|~~]~~~~~~~~~~~~~~~~d\ddddddcdddddd_cddddddddddddddddddddddddddcdd +@HELIUM_000100422_612GNAAXX:7:42:12961:8650#0/2_CONS ali_length=63; direction=left; seq_ab_match=62; sminR=40.0; score=243.985409859; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.87278428347; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +ddddddddddddddddddddddddddddddddddddddddddddd~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddcddddddddddddddddddddddddddddeddddddddddddd +@HELIUM_000100422_612GNAAXX:7:36:10388:10324#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; score=239.950587748; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.87017077012; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgaagtagttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctactacttcgg ++ +dddddddddddddddddddddddddddddddddddddddddddddd~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddeddddddddddddddddddcddddddddddddd +@HELIUM_000100422_612GNAAXX:7:1:8823:6682#0/2_PairEnd sminR=40.0; pairend_limit=108; ali_dir=right; score=15.9839512414; mode=joined; sminL=40.0; +gatcgggagagcgtcgtgtaggggaagagggtagatctcggggggggccgtatcattaaaaaaaaaaacaaacatcagcacgaagcacatgacaccgcctagctatcaatcttgagatgagatcagacatctgtttgttttcttttgtttttttttcaagcagaagacggcatacgatatcggtctcggcattcctgctgaaccgctcttccgatc ++ +BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB_aa]a`]^S``aabaab^bbbbab_c``abbbbbbbcbbbbbbbbbbbbbbbbbbbbbbbbbbab +@HELIUM_000100422_612GNAAXX:7:66:11860:20419#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; score=239.911387587; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.86953850946; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg ++ +ddeddddddddddddedd`dddddd`edddddNddddddddddcdd|~~~b~~~~~~~~~~~~~~~}~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~daddddddddedddddcddddedddddddddddddddddddddedd +@HELIUM_000100422_612GNAAXX:7:3:17077:6562#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; score=223.876050008; seq_a_mismatch=3; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.61090403239; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggcagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg ++ +ccccccccccccccccccccccccbccccccccacccccccccccc~~~ua~~~~~~~~~~~~~~}~~~~~~~a~Fz~zz~~~~~~~~~~~~~~~~~~~~~~~~~~~~badadddddddddddcdddddddcddddddddeddddddbdddbdd +@HELIUM_000100422_612GNAAXX:7:18:11865:6418#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; score=251.959188225; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99935219405; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +ddddddddddddddddddddddddddddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddcdddedddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:19:4311:13343#0/2_CONS ali_length=63; direction=left; seq_ab_match=58; sminR=40.0; score=211.837822462; seq_a_mismatch=4; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.36250511845; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctcgtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +dddddddddddcd`ddcddddddcd\bcccdddddddddcdddcb~b~~~b~~~~~|~~~}~~~{y~~_~~~{Z{~~~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~ddddd^ddcddddcddbdddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:30:1800:7833#0/2_CONS ali_length=62; direction=left; seq_ab_match=52; sminR=40.0; score=167.712615339; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=10; seq_a_deletion=0; score_norm=2.70504218289; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgaagtagttagataccccactatgcttagccctaaacacaagacattaatataacgagattaatcgacagagtactaccggctatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctactacttcgg ++ +DDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDccBccccccccBcBccccBBccBcccccccc~u~v}[wJ~~Q|~~~~~~~~~~~~a~~~~t~Z^ccc`c]bbc_[V_[`[````bb_cc[_cc[\[\[bcbbbccccc +@HELIUM_000100422_612GNAAXX:7:16:19299:8684#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.845804746; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.73944846365; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg ++ +ddddddddddddddddddddddcdddddddddcddddddddddddd~~~~b~~~~~~~`~~~~~~~~~~~~~~~~~~~~~~~~|~~~~~~~~~~~~~~~~~~~~~~~~cadaddddddeddddccdcddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:54:14618:14353#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; score=239.8562981; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.86864996935; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg ++ +ddddddddddddddddddcdddddddddddddcddddddcdddddd~~~~b~~~~~~~~~~~~~~~~~~y~~~~~z|}z~~~~~~~~~~~~~~~~~~~~~~~~~~~~~eddddddddddddddecddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:108:9222:18258#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.857703869; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=2; seq_a_deletion=0; score_norm=3.73964038498; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcagacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg ++ +ddddeeddddddeddddddddddddddddccddddddddddddddd~~~~~~~~~~~~~~~~~~~v~~~~~}~}}~~~~~`~~~~~~~~~~b~~~~~~~~~~~~~~~~dcdddddddddddddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:74:4783:20479#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; score=239.87314657; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.86892171887; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg ++ +dddddddddddddddddd`dddcddcdadcddWdddddadWddbbcqy~~~~~~~~~~r~}~~~qx~~~~~z|~~~~~~~~~~~~~~~}~~~~~~~~~~~~~~~~~b~d`dZddddbddddddaddddddcddddbdddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:38:3005:20881#0/2_CONS ali_length=63; direction=left; seq_ab_match=60; sminR=40.0; score=227.901935995; seq_a_mismatch=3; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.61749104754; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgttcatgtttagggctaagcatagtggggtatctaagatattcgg ++ +dddddddddddddddddddddddddddddddddbdddddcdddbd~~}~~b~~b~~~~~~|~~~~~~~a~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddeadddddddddddddddddddcddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:4:10139:2389#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; score=251.802206044; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99686041339; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +ddddddcddddddddddddd`dcdddddaddaddddbdddddddc~~~~~~~~~{~~~~~~~~zxy~~|~~~~ru~~~~~~~~~~~~~~~~~~{~~p~~~~~~~~~~~ddddddddddabaP`ddddabddddddddddddddddcdcdaddd +@HELIUM_000100422_612GNAAXX:7:75:9674:16966#0/2_CONS ali_length=58; direction=right; seq_ab_match=57; sminR=40.0; score=224.000075421; seq_a_mismatch=0; seq_b_deletion=50; seq_b_mismatch=1; seq_a_deletion=50; score_norm=3.86207026589; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=0; seq_b_single=0; +ccgcctccttagaacaggctcctctagaaaagcatagtggggtatctaaaggaggcgg ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~~~ +@HELIUM_000100422_612GNAAXX:7:59:15367:8376#0/2_CONS ali_length=62; direction=left; seq_ab_match=62; sminR=40.0; score=247.996374666; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99994152687; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgaagtagttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctactacttcgg ++ +ddddddddddddddddddddddddddddddddddddddddddddad~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddedddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:29:1328:13185#0/2_CONS ali_length=63; direction=left; seq_ab_match=59; sminR=40.0; score=223.94591652; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=4; seq_a_deletion=0; score_norm=3.55469708763; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +ddddddddddddccdddddddddeddddddddddddddddedadd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~W[~~~~~~~~~~~~~~~~~~~~~~~b~~~b~dddcdddddddcddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:30:16081:4486#0/2_CONS ali_length=63; direction=left; seq_ab_match=62; sminR=40.0; score=243.950667893; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.87223282369; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +ddddddddddddddddddddddddddddddddddddddcdddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~c~~~dddeddddddddddadddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:104:10274:7999#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; score=251.962499638; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99940475616; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +dddcdddddddddddedddddddddddccdddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddd^ddddddddddddddddddddddddcddddddddddddd +@HELIUM_000100422_612GNAAXX:7:14:7706:12338#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; score=223.864093999; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.61071119354; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; +ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg ++ +dddcddcddcdbdbddddddddddddddddddcdddddccaddddd~~~~~~b~~~~~~~~~~~x~~~~wyZq~mv~}}~~~~~~~~~P~~~~~~~~~~~~~~~~~~~d^cddddddddcdddadddddddddddddddddddddddddddddd diff -r 000000000000 -r aca6a8c9cf83 test-data/input_ngsfilter_extrafile.txt --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/input_ngsfilter_extrafile.txt Wed Apr 12 17:38:00 2017 -0400 @@ -0,0 +1,4 @@ +wolf_diet 13a_F730603 aattaac TTAGATACCCCACTATGC TAGAACAGGCTCCTCTAG F @ +wolf_diet 15a_F730814 gaagtag TTAGATACCCCACTATGC TAGAACAGGCTCCTCTAG F @ +wolf_diet 26a_F040644 gaatatc TTAGATACCCCACTATGC TAGAACAGGCTCCTCTAG F @ +wolf_diet 29a_F260619 gcctcct TTAGATACCCCACTATGC TAGAACAGGCTCCTCTAG F @ diff -r 000000000000 -r aca6a8c9cf83 test-data/output_ngsfilter.fastq --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/output_ngsfilter.fastq Wed Apr 12 17:38:00 2017 -0400 @@ -0,0 +1,952 @@ +@HELIUM_000100422_612GNAAXX:7:119:14871:19157#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=47; sminR=40.0; seq_b_mismatch=13; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=1; mid_quality=69.1492537313; seq_a_deletion=0; seq_b_single=46; score=115.761290673; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=68.8701298701; sminL=40.0; seq_a_single=46; score_norm=1.86711759151; seq_b_insertion=1; +ttagccctaaacacaagtaattattataacaaaatcattcgccagagtactaccggcaatagctcaaaactcaaaggacttggcggtgctttataccctt ++ +cacdddddddddddddc\d~b~~~b~~~~~~b`ryK~|uxyXk`}~ccBBccBcccBcBcccBcBccccccc~~~~b|~~xdbaddaaWcccdaadddda +@HELIUM_000100422_612GNAAXX:7:108:5640:3823#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=2; seq_a_mismatch=0; tail_quality=66.9; seq_b_deletion=0; mid_quality=78.447761194; seq_a_deletion=0; seq_b_single=46; score=231.881364714; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.9545454545; sminL=40.0; seq_a_single=46; score_norm=3.74002201152; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattgttcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddcdddddcddddddd]d~b~~~~~~~~~~~~~Z~~~~~~~~~~~~~~~~~~~~~~~~~~~}~~~~~~~~~~~~~~~~~~ddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:97:14311:19299#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=52; sminR=40.0; seq_b_mismatch=5; seq_a_mismatch=5; tail_quality=67.0; seq_b_deletion=0; mid_quality=75.0820895522; seq_a_deletion=0; seq_b_single=46; score=167.91734192; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.6; avg_quality=74.0064935065; sminL=40.0; seq_a_single=46; score_norm=2.70834422451; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaagagcttaaaactcaaaggacttggcggtgctttataccctt ++ +d`cdddddcd`ddddaa\b~`~~~~~~~~b~~~~]~b~V~~~~~~~~~~~~~~~~`~~^Zs~r|~~~st~|z|v||^~~|TY``accL]`\`daZddddd +@HELIUM_000100422_612GNAAXX:7:22:8540:14708#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=3; seq_a_mismatch=0; tail_quality=66.9; seq_b_deletion=0; mid_quality=78.2537313433; seq_a_deletion=0; seq_b_single=46; score=223.982011431; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.1; avg_quality=76.7922077922; sminL=40.0; seq_a_single=46; score_norm=3.61261308759; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +addbddddddddddddedd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~b~~~~~~~~b~~~~ddddddddddabbb^bddd +@HELIUM_000100422_612GNAAXX:7:57:18459:16145#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=3; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.6917293233; seq_a_deletion=0; seq_b_single=45; score=227.883189945; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=66.9; avg_quality=77.1568627451; sminL=40.0; seq_a_single=45; score_norm=3.61719349119; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~c~~~~~~~~~~~~~~b~~~~~~b~dddddcdddddddddddd +@HELIUM_000100422_612GNAAXX:7:89:10281:5930#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.5373134328; seq_a_deletion=0; seq_b_single=46; score=231.893859399; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.038961039; sminL=40.0; seq_a_single=46; score_norm=3.7402235387; seq_b_insertion=0; +ttagccctaaacacaaataattacacaaacaaaattgttcaccagagtactagcggcaacagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddddddcddddddcdddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~bddddddceddedd\ddddd +@HELIUM_000100422_612GNAAXX:7:29:15520:18035#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=77.828358209; seq_a_deletion=0; seq_b_single=46; score=239.965360923; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=65.6; avg_quality=76.3311688312; sminL=40.0; seq_a_single=46; score_norm=3.87040904714; seq_b_insertion=0; +ttagccctaaacacaagtaattattataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +__`_Y_`_a_`cc_ccccc~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~ddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:6:9274:14951#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=2; seq_a_mismatch=1; tail_quality=67.1; seq_b_deletion=0; mid_quality=78.2089552239; seq_a_deletion=0; seq_b_single=46; score=223.791171196; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.9; avg_quality=76.7532467532; sminL=40.0; seq_a_single=46; score_norm=3.60953501929; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddcddddddddddddddd~~~~~~~~~~~~~~~~~~|z~}a|~~~~}}}}~~~~~~~~~~}~}b~b~~~~~~~~~~~~~~ddddddcdcddcd^ddddd +@HELIUM_000100422_612GNAAXX:7:111:2893:6772#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.7164179104; seq_a_deletion=0; seq_b_single=46; score=239.959941063; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.1948051948; sminL=40.0; seq_a_single=46; score_norm=3.87032163005; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddddddedddddded_d~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~x~dddddddcdddcdcdddad +@HELIUM_000100422_612GNAAXX:7:89:10621:20317#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=3; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=77.5820895522; seq_a_deletion=0; seq_b_single=46; score=223.827654468; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.2077922078; sminL=40.0; seq_a_single=46; score_norm=3.61012345916; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dd`ddddadadddddddRc~}~~z~~~~~~~t~sw}~~g}~~~~~~~~~~~~~~~~~~~~~~~~~~~~^~b`~~~~~~~~~ddddddddbdbdd_dddad +@HELIUM_000100422_612GNAAXX:7:50:2791:11138#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.3805970149; seq_a_deletion=0; seq_b_single=46; score=231.976017167; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.9; avg_quality=76.8961038961; sminL=40.0; seq_a_single=46; score_norm=3.74154866398; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dbdbdddddddc\ddddbd~~~~~~~~~~~~~~~~~~b~~~^~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddbddddd +@HELIUM_000100422_612GNAAXX:7:108:17701:18887#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=58; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=4; tail_quality=66.9; seq_b_deletion=0; mid_quality=77.6492537313; seq_a_deletion=0; seq_b_single=46; score=215.828321765; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.1; avg_quality=76.2012987013; sminL=40.0; seq_a_single=46; score_norm=3.48110196396; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddec`dddddddddddc^c~~~~~~~~~~~~~~xss~~~~~~~~~~~|{|z{~~{~~~b~~~~~b~~~~~~~~~~~b~~~bdddddd\ddddddcddddd +@HELIUM_000100422_612GNAAXX:7:97:10414:9929#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.8134328358; seq_a_deletion=0; seq_b_single=46; score=239.864541102; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.2792207792; sminL=40.0; seq_a_single=46; score_norm=3.86878292101; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddddddddddddedddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~ddddddcddddddcddddd +@HELIUM_000100422_612GNAAXX:7:97:9091:15775#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=3; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.276119403; seq_a_deletion=0; seq_b_single=46; score=223.861916171; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.8116883117; sminL=40.0; seq_a_single=46; score_norm=3.61067606728; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dcdaddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~x~~~~~~~~~|~~~~~~~b~~~~~~^~~b~~~~dddddddddddddcddddd +@HELIUM_000100422_612GNAAXX:7:74:8683:20738#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=62; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.0977443609; seq_a_deletion=0; seq_b_single=45; score=243.961428864; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.5163398693; sminL=40.0; seq_a_single=45; score_norm=3.87240363276; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~`~~~~~~~~~~~~~~ddeddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:24:16230:12703#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=77.4552238806; seq_a_deletion=0; seq_b_single=46; score=223.86908915; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.3; avg_quality=76.0519480519; sminL=40.0; seq_a_single=46; score_norm=3.61079176049; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatcccctt ++ +bdd_ddddaddcbdcdd^b~~~~~b~~~~~~~~~~~~~~~~~~~~~~~~u~y~~~~~~a~~~~~~~~~~~~~~~~{bs~}}a`ddd`a_ddd``Ob`cdd +@HELIUM_000100422_612GNAAXX:7:4:4214:9434#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=58; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=3; tail_quality=63.3; seq_b_deletion=0; mid_quality=76.2388059701; seq_a_deletion=0; seq_b_single=46; score=215.812703412; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=65.3; avg_quality=74.6883116883; sminL=40.0; seq_a_single=46; score_norm=3.48085005502; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactacaggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddbdddddcddddddddd~^~~~{~{~~~~~}~~{~~ym~L||~~wuy~zyxPzjyyvxku~~~~~~~~~~~~~~~~~xbacdddddaddac_^`_`dc +@HELIUM_000100422_612GNAAXX:7:12:17693:3451#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.8507462687; seq_a_deletion=0; seq_b_single=46; score=247.862243; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.3116883117; sminL=40.0; seq_a_single=46; score_norm=3.9977781129; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dedcddddddddddedddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~}v{}~{~}~~~~~~~~~~~~~~~~~~~~~~~~~cdddddcddddddaddddd +@HELIUM_000100422_612GNAAXX:7:3:14624:12375#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=61; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.7669172932; seq_a_deletion=0; seq_b_single=45; score=235.922125108; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.2287581699; sminL=40.0; seq_a_single=45; score_norm=3.74479563663; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dcdcdddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~Y`~~~~~~~~~~~~~~~~~~~~~~~dddddcdddddddceddd +@HELIUM_000100422_612GNAAXX:7:18:5317:11571#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.7462686567; seq_a_deletion=0; seq_b_single=46; score=239.862078449; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.2207792208; sminL=40.0; seq_a_single=46; score_norm=3.86874320079; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddddddddddddeddde~~~~~~~~~~~~~~~~~~~~~~~~~~~~y~~~~~~~~~~~~~~~~~{~~~~~~~~~~b~~~~dddddcdddddddaddddd +@HELIUM_000100422_612GNAAXX:7:109:17335:11429#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=56; sminR=40.0; seq_b_mismatch=6; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=73.1278195489; seq_a_deletion=0; seq_b_single=45; score=195.716358124; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=66.9; avg_quality=72.3202614379; sminL=40.0; seq_a_single=45; score_norm=3.10660885911; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddcdaddddddddcdbd~b~~~~~~~~~|~``}~\`~|[~ccccccccccccccccc~z~~s~x~~~~~}~~}}W~~}x~ddddc]``]\Rd`ddb`^ +@HELIUM_000100422_612GNAAXX:7:7:10646:8487#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=77.671641791; seq_a_deletion=0; seq_b_single=46; score=231.85443709; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.2857142857; sminL=40.0; seq_a_single=46; score_norm=3.739587695; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dccdddddddddddddddec~~~~~~~~~~~~~~~~~~~~~~~~~~~{vztippoph~~~~~~~b~~~~~~~~~~~~~~~~ddddddddddddddddddc +@HELIUM_000100422_612GNAAXX:7:19:4865:20715#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.5074626866; seq_a_deletion=0; seq_b_single=46; score=231.923942025; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.012987013; sminL=40.0; seq_a_single=46; score_norm=3.74070874234; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddcbddddddddddddddb~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~ybddddddddddcddadddcd +@HELIUM_000100422_612GNAAXX:7:13:6954:13039#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.0; seq_a_deletion=0; seq_b_single=45; score=243.872474161; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.431372549; sminL=40.0; seq_a_single=45; score_norm=3.87099165336; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagaggactactagcaatagcttaaaactcaaaggacttggcggtgctttatatccct ++ +dddcdddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~r~~~~~~~~~~~~~~~~~~~~~~~b~~~~~dddddddddddddcdddd +@HELIUM_000100422_612GNAAXX:7:120:14963:1929#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.1492537313; seq_a_deletion=0; seq_b_single=46; score=223.810433242; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.7012987013; sminL=40.0; seq_a_single=46; score_norm=3.60984569745; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddadddddddedddddbd~b~~}~~~~~~~~~~~~~~~~u~~|~~}~~}~~~}z`}}~~~}~~~~~~~~~~~~~~_~~~~dddddddddddddcddddd +@HELIUM_000100422_612GNAAXX:7:115:3921:10649#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=61; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.5864661654; seq_a_deletion=0; seq_b_single=45; score=235.871015095; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.0718954248; sminL=40.0; seq_a_single=45; score_norm=3.74398436659; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +ddedddddddddcddddd~~~~~~~~~~~~~b~~~~~~~~~~~~~~~~~}~}~n{~i~~~~~~~~~~~~~~~~~~~~~~b~dddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:109:12848:8882#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.3805970149; seq_a_deletion=0; seq_b_single=46; score=231.869965661; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.9025974026; sminL=40.0; seq_a_single=46; score_norm=3.73983815582; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddeedddddddddddcd`d~~~~~~~~~~~~~~~~~~~~~~~~~~~~~{~~x~b~~~}~~~~~~~~~~~~~~~~~~~~~~bdcdddddddddddTddddd +@HELIUM_000100422_612GNAAXX:7:84:11257:18886#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=55; sminR=40.0; seq_b_mismatch=8; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=77.1804511278; seq_a_deletion=0; seq_b_single=45; score=187.890540948; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=75.8496732026; sminL=40.0; seq_a_single=45; score_norm=2.98238953885; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddcdddddddddddddd~b~a~~b~~~~b~~~~~~{b~|a~~`\}s~y~~~~~~t~~~~~~~~~~~~~~~~~~~~x~~t~dddddddddddddddddc +@HELIUM_000100422_612GNAAXX:7:72:13060:5051#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.671641791; seq_a_deletion=0; seq_b_single=46; score=239.985823237; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.8; avg_quality=77.1428571429; sminL=40.0; seq_a_single=46; score_norm=3.87073908447; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddddddddddddddd_b~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddeddddd +@HELIUM_000100422_612GNAAXX:7:35:3798:1896#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=15a_F730814; experiment=wolf_diet; forward_tag=gaagtag; reverse_tag=gaagtag; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=66.9; seq_b_deletion=0; mid_quality=78.4701492537; seq_a_deletion=0; seq_b_single=46; score=239.863223783; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.974025974; sminL=40.0; seq_a_single=46; score_norm=3.86876167392; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddddddcddbddddddad~b~~}~~~~~~~~~~~~~~~~~n~~~~~|~}~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddcddaddaddd^dddbc +@HELIUM_000100422_612GNAAXX:7:117:12470:18434#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=59; sminR=40.0; seq_b_mismatch=4; seq_a_mismatch=0; tail_quality=66.9; seq_b_deletion=0; mid_quality=78.045112782; seq_a_deletion=0; seq_b_single=45; score=219.83660627; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=66.9; avg_quality=76.5882352941; sminL=40.0; seq_a_single=45; score_norm=3.4894699408; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddddddddcddddddcd~~~~~~~~~~~~~~~~~~~~~~~~~~~~O~~~~~~~~~{~~{~~Wx~~~~~~~~~~~b~~~b}ddabdddddddddddddd +@HELIUM_000100422_612GNAAXX:7:11:8428:13716#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=15a_F730814; experiment=wolf_diet; forward_tag=gaagtag; reverse_tag=gaagtag; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.7985074627; seq_a_deletion=0; seq_b_single=46; score=239.97387484; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.2662337662; sminL=40.0; seq_a_single=46; score_norm=3.87054636839; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddddddddddddddddad~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:78:18994:16521#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.6940298507; seq_a_deletion=0; seq_b_single=46; score=239.927346216; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.8; avg_quality=77.1623376623; sminL=40.0; seq_a_single=46; score_norm=3.86979590672; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +cddddddcdddddddddad~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~bddaddeeaddded`ddddb +@HELIUM_000100422_612GNAAXX:7:16:5047:7843#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.3007518797; seq_a_deletion=0; seq_b_single=45; score=251.979285107; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.6928104575; sminL=40.0; seq_a_single=45; score_norm=3.99967119217; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dedddbdddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddcdcddddddeec +@HELIUM_000100422_612GNAAXX:7:118:13803:15594#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=60; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.5112781955; seq_a_deletion=0; seq_b_single=45; score=227.87720055; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.0065359477; sminL=40.0; seq_a_single=45; score_norm=3.61709842144; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +edd^ddddbdddddeddd~~~~~~~~~~~~~~~~~~a~~~~~~~~~~~{~y~~~~~~~~~~v~~~~~~~~~~b~~b~~~~~dddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:53:14379:5922#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.0; seq_a_deletion=0; seq_b_single=46; score=247.982071703; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.9; avg_quality=77.4350649351; sminL=40.0; seq_a_single=46; score_norm=3.99971083392; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddddddddddddddd`d~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddeddddd +@HELIUM_000100422_612GNAAXX:7:52:10700:16508#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=64.5; seq_b_deletion=0; mid_quality=77.8805970149; seq_a_deletion=0; seq_b_single=46; score=239.790995416; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.3051948052; sminL=40.0; seq_a_single=46; score_norm=3.86759670025; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +c^ccccccccccacccccc~~~~~~~~~~~~~~{{~~v~~~~~~~~~~~~~~~~}}}~~~~~{~~~~|v|||~}~~~u~~bddddcdbdddbddIddddd +@HELIUM_000100422_612GNAAXX:7:69:7696:12881#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.3082706767; seq_a_deletion=0; seq_b_single=45; score=251.983052061; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.6993464052; sminL=40.0; seq_a_single=45; score_norm=3.99973098509; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +eddddddcdddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddcddd +@HELIUM_000100422_612GNAAXX:7:44:12484:5320#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.1; seq_b_deletion=0; mid_quality=78.5149253731; seq_a_deletion=0; seq_b_single=46; score=231.837799589; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.7; avg_quality=77.0064935065; sminL=40.0; seq_a_single=46; score_norm=3.73931934821; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +deddddddddddddddddc~~~~~~~~~~~~~~~~~~~}}}z~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~adcddddadddddddddddd +@HELIUM_000100422_612GNAAXX:7:21:4713:10229#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.7313432836; seq_a_deletion=0; seq_b_single=46; score=239.864510201; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.2077922078; sminL=40.0; seq_a_single=46; score_norm=3.86878242259; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dcdbddddddddcdddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~|~~~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~~ddddddddddddd`ddddb +@HELIUM_000100422_612GNAAXX:7:38:6201:12003#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=59; sminR=40.0; seq_b_mismatch=4; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=67.7443609023; seq_a_deletion=0; seq_b_single=45; score=219.686655506; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=63.2; avg_quality=67.3986928105; sminL=40.0; seq_a_single=45; score_norm=3.48708976993; seq_b_insertion=0; +ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaatagcttaaaactcaaagaactgggcggtgctttatatcccg ++ +dbddcddcddd[dddddc~~~~b~~~~~~~t~}{axzyz{||zzwzr~y}}}}}z~x~cccccccccccccccccBcccBcDDDDDDDDDDDDDDDDaH +@HELIUM_000100422_612GNAAXX:7:33:16506:1490#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.2; seq_b_deletion=0; mid_quality=79.0373134328; seq_a_deletion=0; seq_b_single=46; score=247.963002972; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.1; avg_quality=77.4935064935; sminL=40.0; seq_a_single=46; score_norm=3.99940327375; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddeddddd +@HELIUM_000100422_612GNAAXX:7:32:4360:3451#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=61; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.7703703704; seq_a_deletion=0; seq_b_single=47; score=243.999335649; seq_a_insertion=0; mode=alignment; seq_length_ori=155; head_quality=67.0; avg_quality=77.2516129032; sminL=40.0; seq_a_single=47; score_norm=3.999989109; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddddddddddddddddde~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddeddddddddddeedddd +@HELIUM_000100422_612GNAAXX:7:115:17402:4174#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=3; tail_quality=67.1; seq_b_deletion=0; mid_quality=77.9402985075; seq_a_deletion=0; seq_b_single=46; score=223.877240886; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.525974026; sminL=40.0; seq_a_single=46; score_norm=3.6109232401; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactacctgcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +edddddccddddedcdd]d~~~~~~~~~~~~~~~~~~~~~~~~~~~~~s~|y~vS~~~~~~{~~~~~~~~~~~~~~b~~~bdddddddddddddcddddd +@HELIUM_000100422_612GNAAXX:7:45:6831:6228#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.0298507463; seq_a_deletion=0; seq_b_single=46; score=247.974637619; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.4675324675; sminL=40.0; seq_a_single=46; score_norm=3.99959092934; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddcddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddeddddd +@HELIUM_000100422_612GNAAXX:7:117:4291:18111#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=62; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.0827067669; seq_a_deletion=0; seq_b_single=45; score=243.911625561; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.5032679739; sminL=40.0; seq_a_single=45; score_norm=3.87161310415; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +ddddddddaddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~dddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:71:14800:3222#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.5597014925; seq_a_deletion=0; seq_b_single=46; score=231.89389474; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.0584415584; sminL=40.0; seq_a_single=46; score_norm=3.74022410871; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddddddeeddddddddcd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~}~~~\~~~~~~~~~~~~~~~~~b~~~~ddddddccdddddcdddde +@HELIUM_000100422_612GNAAXX:7:11:8547:18238#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.2932330827; seq_a_deletion=0; seq_b_single=45; score=251.984413754; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.6862745098; sminL=40.0; seq_a_single=45; score_norm=3.99975259927; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dbdcdddddddddcdddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:117:2416:10342#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.2781954887; seq_a_deletion=0; seq_b_single=45; score=235.921215944; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=76.8039215686; sminL=40.0; seq_a_single=45; score_norm=3.74478120546; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +d^dUYZ[Yddddcdcddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~}~~~~~b|~~b~dddddbddddbddddddd +@HELIUM_000100422_612GNAAXX:7:78:7151:19928#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=56; sminR=40.0; seq_b_mismatch=7; seq_a_mismatch=0; tail_quality=47.6; seq_b_deletion=0; mid_quality=58.2105263158; seq_a_deletion=0; seq_b_single=45; score=195.576462373; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=52.9; avg_quality=57.1699346405; sminL=40.0; seq_a_single=45; score_norm=3.10438829164; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacgagaatgttcgccagtgtacttctagcaacaggctgaaactcaaaggacttggcggtggtttacatccct ++ +WSR]YbbbDDDDDDDDDDcccccccccccBBcccccccccccccBcccccBccccccccccBccccccccccBccccc~`~WNS]Y_KTUVZ[O_SHW] +@HELIUM_000100422_612GNAAXX:7:71:18821:20471#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=53; sminR=40.0; seq_b_mismatch=2; seq_a_mismatch=7; tail_quality=66.9; seq_b_deletion=0; mid_quality=75.9029850746; seq_a_deletion=0; seq_b_single=46; score=175.889514148; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=74.7402597403; sminL=40.0; seq_a_single=46; score_norm=2.83692764755; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dd\ddddddddd^ddd`dd~~~~Xx~~~~~{|q}}x}^Y`J`~Xv{wt~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~sbddddddddddddd^bdccd +@HELIUM_000100422_612GNAAXX:7:58:15837:8531#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.2857142857; seq_a_deletion=0; seq_b_single=45; score=251.96128051; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.6797385621; sminL=40.0; seq_a_single=45; score_norm=3.99938540492; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddbdddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddcddddd +@HELIUM_000100422_612GNAAXX:7:38:10813:16867#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.2406015038; seq_a_deletion=0; seq_b_single=45; score=251.97777927; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.6405228758; sminL=40.0; seq_a_single=45; score_norm=3.99964729; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgtttgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +ddddddddeddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dbcdddddadadcddddd +@HELIUM_000100422_612GNAAXX:7:68:19115:18847#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=54; sminR=40.0; seq_b_mismatch=2; seq_a_mismatch=6; tail_quality=66.6; seq_b_deletion=0; mid_quality=76.0074626866; seq_a_deletion=0; seq_b_single=46; score=183.856571062; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.7; avg_quality=74.7922077922; sminL=40.0; seq_a_single=46; score_norm=2.96542856552; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddecdd_ddcdd_dd^ddd~`~~~~~b~~~~~~~~~~~}~~{b~~~~~~~{~~_~~~s~~s~~~~x~~\\~~~~~~b~~{bd`dcc`P^bb^dbaaaddb +@HELIUM_000100422_612GNAAXX:7:14:3978:11028#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=77.9029850746; seq_a_deletion=0; seq_b_single=46; score=231.869517243; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.8; avg_quality=76.474025974; sminL=40.0; seq_a_single=46; score_norm=3.73983092328; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactacctgcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddeadbdcddddeddd`ad~~~~~~~~~~~~~~~~~~~~~~~~~~~~~w|{u~|J~y~~~v~~~~~~~~~~~~~~~~~~~_dddddddddddbbZ`bcdd +@HELIUM_000100422_612GNAAXX:7:34:18733:3827#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.1052631579; seq_a_deletion=0; seq_b_single=45; score=243.983236898; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.522875817; sminL=40.0; seq_a_single=45; score_norm=3.87274979204; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddddddddddddddddd~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:65:6787:8456#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.5895522388; seq_a_deletion=0; seq_b_single=46; score=231.869006954; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.8; avg_quality=77.0714285714; sminL=40.0; seq_a_single=46; score_norm=3.7398226928; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddddddcddddddedd_d~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~bddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:111:7441:14688#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.015037594; seq_a_deletion=0; seq_b_single=45; score=243.832560428; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.4444444444; sminL=40.0; seq_a_single=45; score_norm=3.87035810203; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddadddddddddedddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~}~~~~~~~~~~~~~~~~~~u~~~~~b~~~~~dddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:86:10092:15563#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=2; tail_quality=66.8; seq_b_deletion=0; mid_quality=78.1940298507; seq_a_deletion=0; seq_b_single=46; score=223.927744132; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.6; avg_quality=76.7012987013; sminL=40.0; seq_a_single=46; score_norm=3.61173780858; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddbdddddccdddddaddd~b~~~~~~~~~~~~~_~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~|bddddddddddcddcddd`^ +@HELIUM_000100422_612GNAAXX:7:16:4418:5128#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=3; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.3656716418; seq_a_deletion=0; seq_b_single=46; score=223.862543287; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.8896103896; sminL=40.0; seq_a_single=46; score_norm=3.61068618206; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddeddddddddddddddb~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~{~~~~~~~~~~~~~~~b~~b~~~bdddeddbdddddddddddd +@HELIUM_000100422_612GNAAXX:7:13:5742:9859#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=4; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=58; seq_ab_match=55; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=3; tail_quality=93.0; seq_b_deletion=50; mid_quality=91.4210526316; seq_a_deletion=50; seq_b_single=0; score=207.846895043; seq_a_insertion=0; mode=alignment; seq_length_ori=58; head_quality=89.4; avg_quality=91.3448275862; sminL=40.0; seq_a_single=0; score_norm=3.58356715591; seq_b_insertion=0; +tttt ++ +~~~~ +@HELIUM_000100422_612GNAAXX:7:85:18986:17662#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=15a_F730814; experiment=wolf_diet; forward_tag=gaagtag; reverse_tag=gaagtag; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=2; seq_a_mismatch=0; tail_quality=67.1; seq_b_deletion=0; mid_quality=78.447761194; seq_a_deletion=0; seq_b_single=46; score=231.874929984; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.9; avg_quality=76.961038961; sminL=40.0; seq_a_single=46; score_norm=3.73991822554; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~}~~s~~~~~b~~~~~~b~~~~~~~ddcddddddcddddddddd +@HELIUM_000100422_612GNAAXX:7:16:16357:19948#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=57; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=5; tail_quality=66.7; seq_b_deletion=0; mid_quality=75.6541353383; seq_a_deletion=0; seq_b_single=45; score=203.860719115; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=62.0; avg_quality=74.1764705882; sminL=40.0; seq_a_single=45; score_norm=3.23588443039; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcacaggacttggcggtgctttacatccct ++ +dd_dbddbdaddbddddd~`~~b~~~~~~b~~{~~~}x}}~}`twyhw~~~~}w|~|~~~~~X~~~|~~~~~Y~~~~~~~~XVRSQ[Qadd``^P^adY +@HELIUM_000100422_612GNAAXX:7:107:3570:18147#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.0820895522; seq_a_deletion=0; seq_b_single=46; score=231.924768153; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=65.9; avg_quality=76.5714285714; sminL=40.0; seq_a_single=46; score_norm=3.74072206698; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcataaaactcaaaggacttggcggtgctttataccctt ++ +dddadddddcddddd\d\d~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~}~~~~~~~~a~s~`ddcd_ddcddddd]ddc_b +@HELIUM_000100422_612GNAAXX:7:82:8836:11354#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.2835820896; seq_a_deletion=0; seq_b_single=46; score=231.781218203; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.8181818182; sminL=40.0; seq_a_single=46; score_norm=3.73840674522; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddedddddddddddddddd~~~~~~{~~~~~~~~~~~~~~~~~~~~~~w{{k~~|~~~~~~~~~~~~~~~~~~~~~b~~~bddddddacdddddcdddda +@HELIUM_000100422_612GNAAXX:7:41:2354:1449#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=55; sminR=40.0; seq_b_mismatch=3; seq_a_mismatch=5; tail_quality=66.4; seq_b_deletion=0; mid_quality=75.5789473684; seq_a_deletion=0; seq_b_single=45; score=187.777927438; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=66.6; avg_quality=74.3921568627; sminL=40.0; seq_a_single=45; score_norm=2.98060202283; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +daaddddddcddbcdddd~b}bb~b~~~b~q~~}~}~x~x~t~s~|k|~w~~z~~{|{~ccBc~~}~~~~~~a~~a~}p~~baceddbbedddda_aa_ +@HELIUM_000100422_612GNAAXX:7:114:4511:17596#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=57; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=4; tail_quality=67.0; seq_b_deletion=0; mid_quality=75.3208955224; seq_a_deletion=0; seq_b_single=46; score=207.883730153; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.2; avg_quality=74.2532467532; sminL=40.0; seq_a_single=46; score_norm=3.35296338956; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtgctaccggcaatagcttaaaactcaaaggacttggcggtgctttatacgctt ++ +ddddddddddddddd^d^d~~~~~~~~~~~~~~~X~~~~~~~~~~~~~W}~~~~~a~sa~~~~~~~~~~~~~~~~~b~~~~ddddddQPPPPPQPKKPPP +@HELIUM_000100422_612GNAAXX:7:94:12367:17729#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=3; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.0; seq_a_deletion=0; seq_b_single=46; score=223.888596169; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.5714285714; sminL=40.0; seq_a_single=46; score_norm=3.61110638983; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddd`ddddddddddeddad~~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~~r|~t||x~~~~~~~~~~~~~~~~~}b~~~bcddddd`dbdadccddecc +@HELIUM_000100422_612GNAAXX:7:30:5374:18213#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.3233082707; seq_a_deletion=0; seq_b_single=45; score=251.958026467; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.7124183007; sminL=40.0; seq_a_single=45; score_norm=3.99933375344; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:49:11740:5547#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=62; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=0; tail_quality=66.6; seq_b_deletion=0; mid_quality=79.1127819549; seq_a_deletion=0; seq_b_single=45; score=243.959827817; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=66.6; avg_quality=77.477124183; sminL=40.0; seq_a_single=45; score_norm=3.87237821931; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +eddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~cddddddddcdddddddd +@HELIUM_000100422_612GNAAXX:7:29:3531:8314#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.8571428571; seq_a_deletion=0; seq_b_single=45; score=235.837856208; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.3071895425; sminL=40.0; seq_a_single=45; score_norm=3.74345803504; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +ddddddddddddddecdd~~~~~~~~~~~~~~~~~~~~~}~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~a~dcddddddddddcddddd +@HELIUM_000100422_612GNAAXX:7:114:10615:11217#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=1; tail_quality=66.9; seq_b_deletion=0; mid_quality=73.7985074627; seq_a_deletion=0; seq_b_single=46; score=231.702876359; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.7; avg_quality=72.8896103896; sminL=40.0; seq_a_single=46; score_norm=3.73714316708; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddddaaa``a_adddd^d~b~~z~~~~~~~~~~~~~cBcccccccccccccc~y~~s~~~~~~~|~~~~~|~~~~sz~~~adddddd[dad`VRW]VZd +@HELIUM_000100422_612GNAAXX:7:108:2729:8600#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.2462686567; seq_a_deletion=0; seq_b_single=46; score=223.856778095; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.7857142857; sminL=40.0; seq_a_single=46; score_norm=3.61059319508; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddddddddddedcdcad~~~~~~~~~~~~~~~a~~~~~~~~~~~~~~~~~~~~~~~`~~~~~~~~~~~}~~~~~b~~~~dddddddcddddWdddddd +@HELIUM_000100422_612GNAAXX:7:103:8847:6326#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.3082706767; seq_a_deletion=0; seq_b_single=45; score=251.968264217; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.6993464052; sminL=40.0; seq_a_single=45; score_norm=3.99949625741; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddcdddd +@HELIUM_000100422_612GNAAXX:7:70:11798:2668#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=15a_F730814; experiment=wolf_diet; forward_tag=gaagtag; reverse_tag=gaagtag; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.8059701493; seq_a_deletion=0; seq_b_single=46; score=239.988667111; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.2727272727; sminL=40.0; seq_a_single=46; score_norm=3.8707849534; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccttt ++ +ddddddddddddddddddd~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dcdddddcddddddddbdd +@HELIUM_000100422_612GNAAXX:7:4:18261:9148#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=61; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.8796992481; seq_a_deletion=0; seq_b_single=45; score=235.937993338; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.1; avg_quality=77.3333333333; sminL=40.0; seq_a_single=45; score_norm=3.7450475133; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +eddddddddddddddddd~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~`~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:29:18416:16620#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=77.4179104478; seq_a_deletion=0; seq_b_single=46; score=231.873033258; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.0649350649; sminL=40.0; seq_a_single=46; score_norm=3.7398876332; seq_b_insertion=0; +ttagccctaaacacaagtaattattataacaaaattattcgccagagttctaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctg ++ +dad\bbbbddcdbdcdd`d~~~~~~~~~~~~~~~~~~~~~~~~~~~~zF||u~s~`~~~~~~~~~~~~~~~~~~~~~~~~~ddddddb^bbb`[Hb`dd\ +@HELIUM_000100422_612GNAAXX:7:32:18262:6966#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=0; tail_quality=66.4; seq_b_deletion=0; mid_quality=78.7537313433; seq_a_deletion=0; seq_b_single=46; score=239.975704028; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.1; avg_quality=77.1948051948; sminL=40.0; seq_a_single=46; score_norm=3.87057587142; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddddddcddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~`~~~~~~~~~~~~ddddddddddbdddddddd +@HELIUM_000100422_612GNAAXX:7:110:17877:11976#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=77.8805970149; seq_a_deletion=0; seq_b_single=46; score=223.833250149; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.4675324675; sminL=40.0; seq_a_single=46; score_norm=3.61021371209; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddcddddddddddd^d\b~`~~~~~~~~~~~~~~~~~~~~x~~~~~~~~~~w{xqv~~~~~~~~~~~~~~~~~~~b~~~bdddddddddddddadddd_ +@HELIUM_000100422_612GNAAXX:7:97:9416:9155#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.5895522388; seq_a_deletion=0; seq_b_single=46; score=231.872797448; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.0844155844; sminL=40.0; seq_a_single=46; score_norm=3.7398838298; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddedddddddddddddde~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~{~~~~~~b~~~~~~~~~~~~~~~bddaddddddddddeddddd +@HELIUM_000100422_612GNAAXX:7:72:8905:10715#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.2932330827; seq_a_deletion=0; seq_b_single=45; score=251.929473647; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.6862745098; sminL=40.0; seq_a_single=45; score_norm=3.99888053408; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddcdddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddcddd +@HELIUM_000100422_612GNAAXX:7:48:5296:16375#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.1278195489; seq_a_deletion=0; seq_b_single=45; score=251.994265764; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.5424836601; sminL=40.0; seq_a_single=45; score_norm=3.99990898038; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +cddbdddddaddddbddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddd`dbdaadddd +@HELIUM_000100422_612GNAAXX:7:53:6550:1163#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.8872180451; seq_a_deletion=0; seq_b_single=45; score=235.996766828; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.3333333333; sminL=40.0; seq_a_single=45; score_norm=3.74598042584; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgtttgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +ddaddddddddddddddd~b~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:120:1900:13295#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.1; seq_b_deletion=0; mid_quality=77.5149253731; seq_a_deletion=1; seq_b_single=46; score=219.874676069; seq_a_insertion=1; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.1558441558; sminL=40.0; seq_a_single=46; score_norm=3.54636574304; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactacgcgcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddcdddddcdddcddddcd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ppthFfpo~~~~~~~~~~~~~~~~~~~b~~~~ddddddcdddddcdddddd +@HELIUM_000100422_612GNAAXX:7:96:19317:7551#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=58; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=3; tail_quality=66.9; seq_b_deletion=0; mid_quality=76.3955223881; seq_a_deletion=0; seq_b_single=46; score=215.85731879; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=65.9; avg_quality=75.0974025974; sminL=40.0; seq_a_single=46; score_norm=3.48156965791; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddbb`dddcdddddddd_d~~~~~~~~~~~~~~~Z~~~~~~~~zzt{qx~~yhpppns_|~~~za~~~~|~~~~~~a|~q~cccccccccccbcXcbccc +@HELIUM_000100422_612GNAAXX:7:93:18556:10479#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.3731343284; seq_a_deletion=0; seq_b_single=46; score=223.913795643; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.9; avg_quality=76.8896103896; sminL=40.0; seq_a_single=46; score_norm=3.61151283295; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddddddddddddddddd~b~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~dddddddddddddbddddd +@HELIUM_000100422_612GNAAXX:7:44:19605:15509#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=3; tail_quality=67.0; seq_b_deletion=0; mid_quality=77.7313432836; seq_a_deletion=0; seq_b_single=46; score=223.916231984; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.3376623377; sminL=40.0; seq_a_single=46; score_norm=3.61155212878; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dadcddddddddddd_d_a~~~~~~~~~~~~~~y}z~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~_~~~p~~`k~`~~r~ddcddcadcdcddcddddd +@HELIUM_000100422_612GNAAXX:7:40:3610:8896#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=15a_F730814; experiment=wolf_diet; forward_tag=gaagtag; reverse_tag=gaagtag; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.828358209; seq_a_deletion=0; seq_b_single=46; score=239.967241748; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.2922077922; sminL=40.0; seq_a_single=46; score_norm=3.87043938304; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~~~dddddddddddddeddddd +@HELIUM_000100422_612GNAAXX:7:41:19763:3556#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=65.7; seq_b_deletion=0; mid_quality=78.5671641791; seq_a_deletion=0; seq_b_single=46; score=247.825851895; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.9805194805; sminL=40.0; seq_a_single=46; score_norm=3.99719115959; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddcddddddddddddcad~~~~~~~~~~~~~~~~~~~~~~~~~~~~{m{zj~~~~~~~~v~~~~~~~~~~~~~~~~~~~{dddddddddddddcddddd +@HELIUM_000100422_612GNAAXX:7:111:7660:7465#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=58; sminR=40.0; seq_b_mismatch=3; seq_a_mismatch=2; tail_quality=66.1; seq_b_deletion=0; mid_quality=76.8646616541; seq_a_deletion=0; seq_b_single=45; score=211.768626837; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=66.5; avg_quality=75.4836601307; sminL=40.0; seq_a_single=45; score_norm=3.36140677518; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +ddcdddddd`ddddddcd~~~~~~~~_~~~~~~~~~~~~~Ky~~~~~~c~Y}~~s~~`~x~}~}~{~Z|h~~~~~~~~~~~dYYdddaddaaddad^dd +@HELIUM_000100422_612GNAAXX:7:76:10822:18722#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=58; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=5; tail_quality=66.8; seq_b_deletion=0; mid_quality=77.3834586466; seq_a_deletion=0; seq_b_single=45; score=211.83390837; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=66.5; avg_quality=75.9803921569; sminL=40.0; seq_a_single=45; score_norm=3.36244299; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacacgaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +acbdcddddTbdcddddd~`~~~z~~~~~~S~~~~~~b~~`~~~t~t~~}~~~~~~~~~~~T~~~|~~~~~~~~~~~~x~~ddddadddd_ddbdcbdd +@HELIUM_000100422_612GNAAXX:7:76:8491:8900#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=57; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=4; tail_quality=65.3; seq_b_deletion=0; mid_quality=76.552238806; seq_a_deletion=0; seq_b_single=46; score=207.828655885; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.9; avg_quality=75.1948051948; sminL=40.0; seq_a_single=46; score_norm=3.35207509492; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtgctacctgcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +bcccccccbcccc_ccb[b~^~~~~~~~~~~~~~uw~~~~}~~~y~~}N~qx~}F}z~~~~~~~~~~~~~o~~~~~b~~~bdddddddddddcdadddda +@HELIUM_000100422_612GNAAXX:7:47:7619:9866#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.1; seq_b_deletion=0; mid_quality=78.8358208955; seq_a_deletion=0; seq_b_single=46; score=239.867362445; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.1; avg_quality=77.3116883117; sminL=40.0; seq_a_single=46; score_norm=3.86882842653; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddddddddddddddded~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~addddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:99:14742:5591#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.5895522388; seq_a_deletion=0; seq_b_single=46; score=231.982488051; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.0844155844; sminL=40.0; seq_a_single=46; score_norm=3.74165303308; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddddddddddddddddcd~b~~~~~~~~~~~~~a~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddddcdd +@HELIUM_000100422_612GNAAXX:7:95:3274:5435#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=61; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=1; tail_quality=66.8; seq_b_deletion=0; mid_quality=78.8421052632; seq_a_deletion=0; seq_b_single=45; score=235.944669323; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.2810457516; sminL=40.0; seq_a_single=45; score_norm=3.74515348131; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddcdddddddddcdddd~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~{~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~ddddddddddcddddddd +@HELIUM_000100422_612GNAAXX:7:74:8396:18135#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.8045112782; seq_a_deletion=0; seq_b_single=45; score=235.858013544; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.2614379085; sminL=40.0; seq_a_single=45; score_norm=3.74377799276; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +ddddddddddddddcddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~W~dddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:9:10358:4089#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=61; seq_ab_match=54; sminR=40.0; seq_b_mismatch=6; seq_a_mismatch=1; tail_quality=66.9; seq_b_deletion=1; mid_quality=66.3880597015; seq_a_deletion=0; seq_b_single=47; score=181.620394786; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.9; avg_quality=66.4545454545; sminL=40.0; seq_a_single=46; score_norm=2.97738352108; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactacaggcaatagctcaaaactcaaagaacttggcggtgctttataccctt ++ +d^dadbaddddddcddcd^`zy~XwzzzZ~~~ccccccccccccccccBccccBccccccccccBcccccccccccBcccacc`ddc][\[]]`addddd +@HELIUM_000100422_612GNAAXX:7:78:10338:12302#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.6119402985; seq_a_deletion=0; seq_b_single=46; score=231.881878067; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.9; avg_quality=77.0974025974; sminL=40.0; seq_a_single=46; score_norm=3.74003029141; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddddddddddddddddd~a~~~~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddedddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:95:17941:6113#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=57; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=6; tail_quality=66.1; seq_b_deletion=0; mid_quality=77.9172932331; seq_a_deletion=0; seq_b_single=45; score=203.869927289; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.1; avg_quality=76.4379084967; sminL=40.0; seq_a_single=45; score_norm=3.23603059189; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +ddd`dd`cdddc^edeca~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~~~~~~~~bb~~~b~~b~~~b~dddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:59:11971:12063#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.9850746269; seq_a_deletion=0; seq_b_single=46; score=247.995051728; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.4285714286; sminL=40.0; seq_a_single=46; score_norm=3.99992018915; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgtcagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +acddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~|~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddcddddd +@HELIUM_000100422_612GNAAXX:7:47:3878:6141#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.0223880597; seq_a_deletion=0; seq_b_single=46; score=247.947034203; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.461038961; sminL=40.0; seq_a_single=46; score_norm=3.99914571295; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:21:5487:19582#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=58; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=3; tail_quality=67.1; seq_b_deletion=0; mid_quality=77.6641791045; seq_a_deletion=0; seq_b_single=46; score=215.831528178; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.5; avg_quality=76.2532467532; sminL=40.0; seq_a_single=46; score_norm=3.4811536803; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dbddcdddcddddddddd_~v}~~}{~~~~b~}ZZ}}}t~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~d`dbdddbdcdcdacdddd +@HELIUM_000100422_612GNAAXX:7:38:13721:2004#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.5298507463; seq_a_deletion=0; seq_b_single=46; score=239.856433937; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.3; avg_quality=76.987012987; sminL=40.0; seq_a_single=46; score_norm=3.86865216027; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +a]ddddddddddddddd`d~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~}}~~~~~~~~~~~~~~~b~~~~~~~~~~~~{~dddddddddbadd^dbddb +@HELIUM_000100422_612GNAAXX:7:111:19168:18517#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=15a_F730814; experiment=wolf_diet; forward_tag=gaagtag; reverse_tag=gaagtag; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=60; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=77.8955223881; seq_a_deletion=0; seq_b_single=46; score=223.825469474; seq_a_insertion=1; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.4805194805; sminL=40.0; seq_a_single=45; score_norm=3.55278522975; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattatccgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddbddddddbcdddd^dd~~~{~_~~~~~~~~~y{}{~U~{|~~~~~n~~|~~~~}x~~~~~~~~~~~~~~~~~^~~~~~~ccdaddbdddbddb_dddd +@HELIUM_000100422_612GNAAXX:7:89:16162:8889#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.1278195489; seq_a_deletion=0; seq_b_single=45; score=243.971622168; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.2; avg_quality=77.5555555556; sminL=40.0; seq_a_single=45; score_norm=3.87256543123; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddddddddddddddddd~~c~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddedddddddeddddd +@HELIUM_000100422_612GNAAXX:7:74:4888:6953#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=61; sminR=40.0; seq_b_mismatch=2; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.8571428571; seq_a_deletion=0; seq_b_single=45; score=235.986171514; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.3071895425; sminL=40.0; seq_a_single=45; score_norm=3.74581224625; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~]~~~~~~~~~~~~~~~~~~~dddddddddedddddddd +@HELIUM_000100422_612GNAAXX:7:41:16707:19819#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.3656716418; seq_a_deletion=0; seq_b_single=46; score=231.94731954; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.1; avg_quality=76.8961038961; sminL=40.0; seq_a_single=46; score_norm=3.74108579903; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddddddddddddddddbb~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~bddad`a^dddbddaddddd +@HELIUM_000100422_612GNAAXX:7:7:7916:16505#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=4; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=58; seq_ab_match=58; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=93.0; seq_b_deletion=50; mid_quality=93.0; seq_a_deletion=50; seq_b_single=0; score=231.999536374; seq_a_insertion=0; mode=alignment; seq_length_ori=58; head_quality=93.0; avg_quality=93.0; sminL=40.0; seq_a_single=0; score_norm=3.99999200644; seq_b_insertion=0; +tttt ++ +~~~~ +@HELIUM_000100422_612GNAAXX:7:41:9316:2256#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.6343283582; seq_a_deletion=0; seq_b_single=46; score=239.993848494; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.1233766234; sminL=40.0; seq_a_single=46; score_norm=3.87086852409; seq_b_insertion=0; +ttagccctaaacacaagaaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddddddddddddddddNd~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddcadeddddd +@HELIUM_000100422_612GNAAXX:7:67:7582:3642#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=62; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.0902255639; seq_a_deletion=0; seq_b_single=45; score=243.962430842; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.5098039216; sminL=40.0; seq_a_single=45; score_norm=3.87241953718; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~`~~~~~~~~dddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:42:8435:4155#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.2857142857; seq_a_deletion=0; seq_b_single=45; score=251.987540492; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.1; avg_quality=77.6862745098; sminL=40.0; seq_a_single=45; score_norm=3.99980223003; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +ddddddddddd`dddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:33:9900:9541#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.8955223881; seq_a_deletion=0; seq_b_single=46; score=247.949713348; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.3506493506; sminL=40.0; seq_a_single=46; score_norm=3.99918892497; seq_b_insertion=0; +ctagccctaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddddd\`cccccdddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddedddddddddecddcdd +@HELIUM_000100422_612GNAAXX:7:48:7139:3789#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.3157894737; seq_a_deletion=0; seq_b_single=45; score=251.902451987; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.7058823529; sminL=40.0; seq_a_single=45; score_norm=3.99845161885; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:46:13269:4134#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.3157894737; seq_a_deletion=0; seq_b_single=45; score=251.997344433; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.7058823529; sminL=40.0; seq_a_single=45; score_norm=3.99995784814; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddcddddddddddd +@HELIUM_000100422_612GNAAXX:7:46:9246:19171#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=52; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=10; tail_quality=65.2; seq_b_deletion=0; mid_quality=70.7611940299; seq_a_deletion=0; seq_b_single=46; score=167.802718427; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.7; avg_quality=70.1363636364; sminL=40.0; seq_a_single=46; score_norm=2.70649545851; seq_b_insertion=0; +ttagccctaaacacaagtaattattataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +`dddddddddd^bddadcd~b~~~~~b~~~~~~~_`~~~~~b~~~~~S~~~~~~~~~~b~~n~`~~k~zoY|~~~~jb~~~DDDDDDDDDDDDDDDDDD` +@HELIUM_000100422_612GNAAXX:7:37:12979:6595#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=4; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=58; seq_ab_match=58; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=93.0; seq_b_deletion=50; mid_quality=93.0; seq_a_deletion=50; seq_b_single=0; score=231.998796685; seq_a_insertion=0; mode=alignment; seq_length_ori=58; head_quality=93.0; avg_quality=93.0; sminL=40.0; seq_a_single=0; score_norm=3.99997925319; seq_b_insertion=0; +tttt ++ +~~~~ +@HELIUM_000100422_612GNAAXX:7:95:1688:9598#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=54; sminR=40.0; seq_b_mismatch=3; seq_a_mismatch=6; tail_quality=66.8; seq_b_deletion=0; mid_quality=74.2932330827; seq_a_deletion=0; seq_b_single=45; score=179.847313607; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=65.7; avg_quality=73.2418300654; sminL=40.0; seq_a_single=45; score_norm=2.8547192636; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtgatactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddc`ddddcda`bcabb~~[~~~~~~~~~~~~~__~~|z~}}}~~SPF|mxxrwwtIwjxwvv{p~rwxzx`~~Wp~~~~d[ddd`[`^^dcdddd_d +@HELIUM_000100422_612GNAAXX:7:55:16528:11418#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=58; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=4; tail_quality=57.0; seq_b_deletion=0; mid_quality=76.0746268657; seq_a_deletion=0; seq_b_single=46; score=215.988419267; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.5; avg_quality=74.2142857143; sminL=40.0; seq_a_single=46; score_norm=3.48368418172; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcactagcttacaactcaaaggacttggcggtgctttataccctt ++ +ddcdddddddddddcd`da~~~~~~~~~~~~x~~~~~\~~~~~~~~~v~~~~~~~~~~\~~~~~~~[~~~s~~~~~~~~]~cY__]\QPPPP`]`[`c^^ +@HELIUM_000100422_612GNAAXX:7:32:18486:7018#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.3233082707; seq_a_deletion=0; seq_b_single=45; score=251.909476689; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.1; avg_quality=77.7189542484; sminL=40.0; seq_a_single=45; score_norm=3.99856312205; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +ddddddddddddddedcd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddedddddddd +@HELIUM_000100422_612GNAAXX:7:85:2105:21152#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=57; sminR=40.0; seq_b_mismatch=4; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=73.8947368421; seq_a_deletion=0; seq_b_single=45; score=203.706262238; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=72.9934640523; sminL=40.0; seq_a_single=45; score_norm=3.23343273394; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +daddddddddddaddd`d~b~~~~b~s`~b~~~~~~~~~j~x~~~cccccccccccccccccc~~~~\}~}~~~~~{~~b~dbddddedddeddcdddd +@HELIUM_000100422_612GNAAXX:7:83:6795:14061#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.9328358209; seq_a_deletion=0; seq_b_single=46; score=247.992382324; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.3831168831; sminL=40.0; seq_a_single=46; score_norm=3.99987713426; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddddddddddddddd_d~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddcddddddddddbdddbd +@HELIUM_000100422_612GNAAXX:7:73:16525:10764#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.3059701493; seq_a_deletion=0; seq_b_single=46; score=231.90336822; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.9; avg_quality=76.8311688312; sminL=40.0; seq_a_single=46; score_norm=3.74037690678; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +c_dddddddcddcdddd^b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~|~~~~~b~b~~~~~~~~~~~~~~dddddd_bddddb[dddda +@HELIUM_000100422_612GNAAXX:7:19:6313:16567#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=3; tail_quality=67.0; seq_b_deletion=0; mid_quality=77.4135338346; seq_a_deletion=0; seq_b_single=45; score=227.826165596; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=66.6; avg_quality=76.0261437908; sminL=40.0; seq_a_single=45; score_norm=3.61628834279; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaagggcttggcggtgctttacatccct ++ +ddddcddddddacddddd~~~~~~~~~~~~~~~~~~~~~}}w|~r~xvwrm{z~~~|a~~~~~~~}u~~}~~~~~_O~~~~ddcddb_b__`baYdcdd +@HELIUM_000100422_612GNAAXX:7:114:8865:19510#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.8059701493; seq_a_deletion=0; seq_b_single=46; score=239.913762365; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.1; avg_quality=77.2792207792; sminL=40.0; seq_a_single=46; score_norm=3.86957681233; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddddddddddddddddd~a~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddcddddddd +@HELIUM_000100422_612GNAAXX:7:111:8478:1127#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=57; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=5; tail_quality=67.0; seq_b_deletion=0; mid_quality=76.3909774436; seq_a_deletion=0; seq_b_single=45; score=203.794981474; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=66.4; avg_quality=75.1241830065; sminL=40.0; seq_a_single=45; score_norm=3.23484097578; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctggaacgcaaaggacttggcggtgctttacatccct ++ +dddaddddddddddMbdd~b~~~|~~~~~~{~~~~~~~zy|~~~~{~pv}y~~|~~~~~~~}~|~HxxwIup`~~Z~~v]~adddddacdddbad^dcd +@HELIUM_000100422_612GNAAXX:7:81:15726:2345#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=56; sminR=40.0; seq_b_mismatch=7; seq_a_mismatch=0; tail_quality=62.3; seq_b_deletion=0; mid_quality=63.1954887218; seq_a_deletion=0; seq_b_single=45; score=195.641193686; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=62.5; avg_quality=63.091503268; sminL=40.0; seq_a_single=45; score_norm=3.10541577279; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaagcactcggcggtgctttacatccct ++ +WJXc_bcca_ab__cc_b~~s~~{~~sww~pq~~~~cBccBccccccBcBccccccccccBccccccccccccccBcccBcDDDDDDDDDD`cbQc___ +@HELIUM_000100422_612GNAAXX:7:13:14386:20412#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=60; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.4552238806; seq_a_deletion=0; seq_b_single=45; score=223.918527801; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.9675324675; sminL=40.0; seq_a_single=46; score_norm=3.55426234604; seq_b_insertion=1; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +decddddddddddddddd~~a~~~~xa~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~t~~~~~~~~~~~~~~~~~~b~~~~ddddddddddddddddddc +@HELIUM_000100422_612GNAAXX:7:83:18882:9967#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=60; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.6466165414; seq_a_deletion=0; seq_b_single=45; score=227.922890965; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.1241830065; sminL=40.0; seq_a_single=45; score_norm=3.6178236661; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddddddddddddddddd~b~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~~ddddddddddddddddda +@HELIUM_000100422_612GNAAXX:7:13:17353:13234#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=2; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=77.6417910448; seq_a_deletion=0; seq_b_single=46; score=223.801902424; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.1; avg_quality=76.2662337662; sminL=40.0; seq_a_single=46; score_norm=3.60970810361; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccttt ++ +ddddddddddddeddWd`b~~}~~~~~x~~~~~oVp]~~~~~~~~~~~~~|~~~~~~~~~~~~~~~~~~~~~~~~~~~~~bdaddbddbdddbcUcbddd +@HELIUM_000100422_612GNAAXX:7:53:10153:11845#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=15a_F730814; experiment=wolf_diet; forward_tag=gaagtag; reverse_tag=gaagtag; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.8059701493; seq_a_deletion=0; seq_b_single=46; score=239.985596162; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.2727272727; sminL=40.0; seq_a_single=46; score_norm=3.87073542196; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddddddddddddddddd~~~~~~~~~~~~~~~a~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddeddddd +@HELIUM_000100422_612GNAAXX:7:33:9296:13526#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.5597014925; seq_a_deletion=0; seq_b_single=46; score=239.852481933; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.7; avg_quality=77.038961039; sminL=40.0; seq_a_single=46; score_norm=3.86858841828; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dcdddddeedddddddd^d~~~~~~~~~~~~~~~~~~~~~~~~~~~~~|~|~~~~~v~~~~~~~~~~~~~~~~~~~~~~~^dddddddbdddddZdddcd +@HELIUM_000100422_612GNAAXX:7:113:2918:18736#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=58; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=4; tail_quality=67.0; seq_b_deletion=0; mid_quality=77.828358209; seq_a_deletion=0; seq_b_single=46; score=215.87931956; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.4220779221; sminL=40.0; seq_a_single=46; score_norm=3.48192450903; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dcdcdddddddddddd_`d~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~v~~~v~~y`~~y~~~~~~~~~~~b~~b~~~bdcddddcddddda_ddddd +@HELIUM_000100422_612GNAAXX:7:63:4725:12502#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.0671641791; seq_a_deletion=0; seq_b_single=46; score=239.998975242; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.5; avg_quality=76.5974025974; sminL=40.0; seq_a_single=46; score_norm=3.87095121358; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dcdcddeddddddddWd]d~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~~dddddd``ddd]\R]\ddT +@HELIUM_000100422_612GNAAXX:7:7:11110:17924#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=15a_F730814; experiment=wolf_diet; forward_tag=gaagtag; reverse_tag=gaagtag; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=58; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=3; tail_quality=67.0; seq_b_deletion=0; mid_quality=75.723880597; seq_a_deletion=0; seq_b_single=46; score=215.812113201; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.4; avg_quality=74.5519480519; sminL=40.0; seq_a_single=46; score_norm=3.4808405355; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtcataccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dcddddddddcdddddd`d~b~~~~~~~~~~~~~~~~~rm{kripoooEJpoolppp~~~~~~~~~~~~~v~~~~~`v~~~dddcdddddddbddddadd +@HELIUM_000100422_612GNAAXX:7:102:6364:4627#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.2932330827; seq_a_deletion=0; seq_b_single=45; score=251.985885517; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.6862745098; sminL=40.0; seq_a_single=45; score_norm=3.99977596059; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddcddddddddddcdddd +@HELIUM_000100422_612GNAAXX:7:96:8737:4973#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=4; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=58; seq_ab_match=57; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=93.0; seq_b_deletion=50; mid_quality=92.2631578947; seq_a_deletion=50; seq_b_single=0; score=223.980140585; seq_a_insertion=0; mode=alignment; seq_length_ori=58; head_quality=93.0; avg_quality=92.5172413793; sminL=40.0; seq_a_single=0; score_norm=3.86172656181; seq_b_insertion=0; +tttt ++ +~b~~ +@HELIUM_000100422_612GNAAXX:7:89:9710:20652#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=62; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.8421052632; seq_a_deletion=0; seq_b_single=45; score=243.910293117; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.2941176471; sminL=40.0; seq_a_single=45; score_norm=3.87159195424; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttatatccct ++ +dad]ccccddcdddcddd~~}~~~~~~~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~}ddaddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:71:17473:7401#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=77.2089552239; seq_a_deletion=0; seq_b_single=46; score=231.883938289; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=65.5; avg_quality=75.7857142857; sminL=40.0; seq_a_single=46; score_norm=3.74006352079; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggtaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddaddddccdbddbddd~~~~~~~~b~~~~~~~~~~~~~~~~~~~~~~~~wywop~~~~gx~~~~~w~~s~~~~R~~~~c^cc_ccccc^caabaccc +@HELIUM_000100422_612GNAAXX:7:28:9406:18226#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.4925373134; seq_a_deletion=0; seq_b_single=46; score=231.816671723; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.0; sminL=40.0; seq_a_single=46; score_norm=3.73897857618; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddddddddddcdcdecd~~~~~~~~~~~~~~~~|~~~~~~~~~~~~~~{~~~~~~~~~~x~~~~~~~~~~~~~~b~~~_ddeddddddddddcddddd +@HELIUM_000100422_612GNAAXX:7:15:16118:15111#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=15a_F730814; experiment=wolf_diet; forward_tag=gaagtag; reverse_tag=gaagtag; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.4104477612; seq_a_deletion=0; seq_b_single=46; score=231.917355899; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.9285714286; sminL=40.0; seq_a_single=46; score_norm=3.7406025145; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddddddddddddd`d]d~~~c~~~~x~~~~~~a~~~w~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddd`dddeddddd +@HELIUM_000100422_612GNAAXX:7:58:18618:20959#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=2; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.2537313433; seq_a_deletion=0; seq_b_single=46; score=223.882241304; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.1; avg_quality=76.7987012987; sminL=40.0; seq_a_single=46; score_norm=3.611003892; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddcddddddddddddddddb~~~~~b~~~~~~~~~x~~z~~~~~|~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b}~~~ddddddcdddddd_ddddd +@HELIUM_000100422_612GNAAXX:7:46:11654:6037#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.4402985075; seq_a_deletion=0; seq_b_single=46; score=239.828425953; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.9545454545; sminL=40.0; seq_a_single=46; score_norm=3.8682004186; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddedddcdddddddddcd~~~~~~~~~~~~~~~~~~~~~~~~~~~}{z~~v~~~{~~~~~~~}~~~~~~~~~~~~~~~~bcccddcb^bbbcbdddddd +@HELIUM_000100422_612GNAAXX:7:2:6320:8068#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.3082706767; seq_a_deletion=0; seq_b_single=45; score=251.910304163; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.1; avg_quality=77.7058823529; sminL=40.0; seq_a_single=45; score_norm=3.99857625655; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddeddddbddddddd +@HELIUM_000100422_612GNAAXX:7:46:1592:11866#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=66.5; seq_b_deletion=0; mid_quality=78.6791044776; seq_a_deletion=0; seq_b_single=46; score=247.993604064; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.8; avg_quality=77.1168831169; sminL=40.0; seq_a_single=46; score_norm=3.99989683974; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dd_dddbcd\dddddd]dd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddcddcddd`dddcddcdd +@HELIUM_000100422_612GNAAXX:7:119:2660:19129#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=61; sminR=40.0; seq_b_mismatch=2; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.6541353383; seq_a_deletion=0; seq_b_single=45; score=235.859804076; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.1307189542; sminL=40.0; seq_a_single=45; score_norm=3.74380641391; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dcddcddddddddcdddd~~~~~~~~~~~~~~~~z}~~~~~~~~~~~~~~~~~~~~~~~~~~~~k~~~~~~~~~~b~^~~~dddbdddddddddddddd +@HELIUM_000100422_612GNAAXX:7:115:3625:10051#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=5; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=59; seq_ab_match=59; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=93.0; seq_b_deletion=49; mid_quality=93.0; seq_a_deletion=49; seq_b_single=0; score=235.999642017; seq_a_insertion=0; mode=alignment; seq_length_ori=59; head_quality=93.0; avg_quality=93.0; sminL=40.0; seq_a_single=0; score_norm=3.99999393249; seq_b_insertion=0; +caata ++ +~~~~~ +@HELIUM_000100422_612GNAAXX:7:100:17253:5513#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=65.1; seq_b_deletion=0; mid_quality=76.6417910448; seq_a_deletion=0; seq_b_single=46; score=231.761122002; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.7; avg_quality=75.2467532468; sminL=40.0; seq_a_single=46; score_norm=3.73808261294; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddbdddddcddddddddd~~~~|{|~~~~b~~~~~~~~~~s~~~~~x~~~~~~~rs~zqwxy~z~~|}~z~}|{^v~~~~c``Zc`cXWcc[WYYZccc +@HELIUM_000100422_612GNAAXX:7:82:6036:6921#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=2; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.4104477612; seq_a_deletion=0; seq_b_single=46; score=231.901981419; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.9285714286; sminL=40.0; seq_a_single=46; score_norm=3.74035453901; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddcbddddeddddddddcd~~~~~b~~~~~~~~~~~~~~~~~~~~~~~]~~~~~~~~}~~~~~~~~~~~~~~~~~~~|~{~dddddccdcddddabddd` +@HELIUM_000100422_612GNAAXX:7:120:4726:20808#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.6940298507; seq_a_deletion=0; seq_b_single=46; score=247.810697755; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.9; avg_quality=77.1688311688; sminL=40.0; seq_a_single=46; score_norm=3.99694673798; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dbedddddcdddddddddb~~~~~~~~~~~~~~ty}y~~~~~~~~~~~~{~~~~~~~~~~~~~~~~~~~~~~~~~~~~~r~ddddcdddddcdd`ddddd +@HELIUM_000100422_612GNAAXX:7:99:8183:13912#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=1; tail_quality=67.1; seq_b_deletion=0; mid_quality=78.3358208955; seq_a_deletion=0; seq_b_single=46; score=231.903850642; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.8701298701; sminL=40.0; seq_a_single=46; score_norm=3.74038468777; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctc ++ +ddddddddddcddddddeb~Z~~~~~~~~~~~~~~~~~}~l~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~dadcdedddcdddddddd` +@HELIUM_000100422_612GNAAXX:7:10:4085:7492#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=62; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.1052631579; seq_a_deletion=0; seq_b_single=45; score=243.95581367; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.522875817; sminL=40.0; seq_a_single=45; score_norm=3.87231450269; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~dddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:35:12723:18557#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.1804511278; seq_a_deletion=0; seq_b_single=45; score=251.945522222; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.5882352941; sminL=40.0; seq_a_single=45; score_norm=3.99913527337; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +adddcddddddddddddd~~}~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~cdddcdddddddbddddd +@HELIUM_000100422_612GNAAXX:7:97:10495:17046#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=0; tail_quality=66.9; seq_b_deletion=0; mid_quality=78.7686567164; seq_a_deletion=0; seq_b_single=46; score=239.938979134; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.1; avg_quality=77.2402597403; sminL=40.0; seq_a_single=46; score_norm=3.86998353443; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~a~~~~~~~~~~~~dddddddddddddddcddd +@HELIUM_000100422_612GNAAXX:7:73:17068:16620#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=4; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=58; seq_ab_match=57; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=0; tail_quality=92.8; seq_b_deletion=50; mid_quality=92.2631578947; seq_a_deletion=50; seq_b_single=0; score=223.962007947; seq_a_insertion=0; mode=alignment; seq_length_ori=58; head_quality=93.0; avg_quality=92.4827586207; sminL=40.0; seq_a_single=0; score_norm=3.86141393012; seq_b_insertion=0; +tttt ++ +~~~~ +@HELIUM_000100422_612GNAAXX:7:23:13304:5167#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.0149253731; seq_a_deletion=0; seq_b_single=46; score=247.997553566; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.4545454545; sminL=40.0; seq_a_single=46; score_norm=3.99996054139; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddcddddd +@HELIUM_000100422_612GNAAXX:7:54:2542:19180#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.7313432836; seq_a_deletion=0; seq_b_single=46; score=239.868258533; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.2077922078; sminL=40.0; seq_a_single=46; score_norm=3.86884287957; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddddddddddddddbdad~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~|~~~~~~~~~~~~~~~~~b~~~~dddddccdcddddbddddd +@HELIUM_000100422_612GNAAXX:7:109:17917:15159#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.8571428571; seq_a_deletion=0; seq_b_single=45; score=235.857230348; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.1; avg_quality=77.3137254902; sminL=40.0; seq_a_single=45; score_norm=3.74376556108; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddcdddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~}~~~~~~~~~~~~~~~~~~~~~a~~b~~~~~cddddddddddddcdddd +@HELIUM_000100422_612GNAAXX:7:59:18371:8149#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.0149253731; seq_a_deletion=0; seq_b_single=46; score=247.998742319; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.4545454545; sminL=40.0; seq_a_single=46; score_norm=3.99997971482; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dcddddddddddddddddc~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddeddddd +@HELIUM_000100422_612GNAAXX:7:86:11013:15234#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.8208955224; seq_a_deletion=0; seq_b_single=46; score=239.990009212; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.2857142857; sminL=40.0; seq_a_single=46; score_norm=3.8708066002; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddddddddddddddddd~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:77:18518:10758#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=2; tail_quality=62.5; seq_b_deletion=0; mid_quality=72.2388059701; seq_a_deletion=0; seq_b_single=46; score=223.70605163; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=63.9; avg_quality=71.0649350649; sminL=40.0; seq_a_single=46; score_norm=3.60816212307; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +caccaccccccccccc_cc~~~~~~~~~~~~wzz{~~a~~~~y~_~~~~~~~~~~m~sygwqv~ccBccccccccccccc~bb^^bc`U\\`\]XPZ\__ +@HELIUM_000100422_612GNAAXX:7:63:18851:7688#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=3; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.4104477612; seq_a_deletion=0; seq_b_single=46; score=223.854412144; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.9285714286; sminL=40.0; seq_a_single=46; score_norm=3.61055503459; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dcddddddddddedddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~a~b~~~~~~~~~b~~~~dddddddddddeddddddd +@HELIUM_000100422_612GNAAXX:7:22:1798:3790#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=3; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.3208955224; seq_a_deletion=0; seq_b_single=46; score=223.82253574; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.8506493506; sminL=40.0; seq_a_single=46; score_norm=3.61004089903; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtgctaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dadbddbdddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~~~~~b~~~bdddddddddddddaddddd +@HELIUM_000100422_612GNAAXX:7:115:14354:10118#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=59; sminR=40.0; seq_b_mismatch=2; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.045112782; seq_a_deletion=0; seq_b_single=45; score=219.850378027; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=76.6013071895; sminL=40.0; seq_a_single=45; score_norm=3.4896885401; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactcgcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +ddeddddddddeddddcd~b~~~~~~~~~~~~_~~~~~~~~~~~~~~~w|y~tJ}~~~~~~}~~~~~~~~~~~~~b~~~~~ddddddddcddccddddd +@HELIUM_000100422_612GNAAXX:7:99:14794:3606#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=61; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.7894736842; seq_a_deletion=0; seq_b_single=45; score=235.971679218; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=66.9; avg_quality=77.2418300654; sminL=40.0; seq_a_single=45; score_norm=3.74558220981; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddddddddddddddddd~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~dddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:74:8297:20108#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=15a_F730814; experiment=wolf_diet; forward_tag=gaagtag; reverse_tag=gaagtag; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.776119403; seq_a_deletion=0; seq_b_single=46; score=239.962155962; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.2467532468; sminL=40.0; seq_a_single=46; score_norm=3.87035735423; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddcdddddddddddddd`d~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~}~ddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:29:12308:10119#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.3233082707; seq_a_deletion=0; seq_b_single=45; score=251.866768717; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.7124183007; sminL=40.0; seq_a_single=45; score_norm=3.99788521773; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddddddeddddedcddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:25:11691:21134#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.1567164179; seq_a_deletion=0; seq_b_single=46; score=223.86269299; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.7077922078; sminL=40.0; seq_a_single=46; score_norm=3.61068859661; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddcdddddcddddddad]d~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~}~}~~~~~~~~~~~~~~~}~~b~~~_dddddddadddcdaddddd +@HELIUM_000100422_612GNAAXX:7:111:15484:19248#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=60; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=77.954887218; seq_a_deletion=0; seq_b_single=45; score=227.828264164; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=76.522875817; sminL=40.0; seq_a_single=45; score_norm=3.6163216534; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +ddddddddddddddaddd~~~~~~~~~~~~~~~`~~jzvxt{~z~~~{~}yx~}~~{`~~~~~~~~s~~~~~~~~b~~~~~dddddddddd`ddcdddd +@HELIUM_000100422_612GNAAXX:7:103:3594:15996#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.3805970149; seq_a_deletion=0; seq_b_single=46; score=231.92194325; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.1; avg_quality=76.9090909091; sminL=40.0; seq_a_single=46; score_norm=3.74067650404; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacacaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddddddddddddddddddb~~~~~~~~~~~R~}~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddaddddd +@HELIUM_000100422_612GNAAXX:7:1:14576:14181#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=15a_F730814; experiment=wolf_diet; forward_tag=gaagtag; reverse_tag=gaagtag; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.5373134328; seq_a_deletion=0; seq_b_single=46; score=223.962708229; seq_a_insertion=1; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.038961039; sminL=40.0; seq_a_single=45; score_norm=3.55496362269; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddddddcddddddcda~~~~b~~~~~~~~~~~a~~~~~~`~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddedddddcddddd +@HELIUM_000100422_612GNAAXX:7:99:13467:17714#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=2; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.3432835821; seq_a_deletion=0; seq_b_single=46; score=223.932198733; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.9; avg_quality=76.8636363636; sminL=40.0; seq_a_single=46; score_norm=3.61180965699; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddcddddddddddddddcd~~~~~~~~b~~~}~~~~~~~~~~~~~~~~~~~~~~~^~~~~~~~~~~~~~~~~~~~~~~~~bddddddddddeddcddddd +@HELIUM_000100422_612GNAAXX:7:43:7980:7908#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.5970149254; seq_a_deletion=0; seq_b_single=46; score=239.984831014; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.9; avg_quality=77.0844155844; sminL=40.0; seq_a_single=46; score_norm=3.87072308087; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +daddcdddd_cddcdddda~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~`~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~d`ddddddddddccbdddd +@HELIUM_000100422_612GNAAXX:7:105:14135:6905#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.8134328358; seq_a_deletion=0; seq_b_single=46; score=239.985379811; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.2792207792; sminL=40.0; seq_a_single=46; score_norm=3.87073193244; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcaccagagtactagcggcaacagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddddddddddddddddd~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddeddddddddddddbddd +@HELIUM_000100422_612GNAAXX:7:89:16663:15081#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.0149253731; seq_a_deletion=0; seq_b_single=46; score=247.939062327; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.1; avg_quality=77.461038961; sminL=40.0; seq_a_single=46; score_norm=3.9990171343; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddecddddd +@HELIUM_000100422_612GNAAXX:7:86:15907:21191#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=58; sminR=40.0; seq_b_mismatch=2; seq_a_mismatch=3; tail_quality=67.0; seq_b_deletion=0; mid_quality=77.9172932331; seq_a_deletion=0; seq_b_single=45; score=211.91203832; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=66.9; avg_quality=76.4836601307; sminL=40.0; seq_a_single=45; score_norm=3.36368314793; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dd`deddddddddddddd~~~~~{~~~~~~~~~~~~~~~~~~~~~~~~X~~~[~~~~~~~~~~~~~~~~~~~`~{a~~~b~ddcd\ddadddddcdddb +@HELIUM_000100422_612GNAAXX:7:81:17261:2872#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.1; seq_b_deletion=0; mid_quality=79.3157894737; seq_a_deletion=0; seq_b_single=45; score=251.967130674; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=66.9; avg_quality=77.7058823529; sminL=40.0; seq_a_single=45; score_norm=3.99947826467; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddedddddddddedddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddadddddeddedddddd +@HELIUM_000100422_612GNAAXX:7:14:15397:17819#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.171641791; seq_a_deletion=0; seq_b_single=46; score=223.847163266; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.9; avg_quality=76.7142857143; sminL=40.0; seq_a_single=46; score_norm=3.61043811719; seq_b_insertion=0; +ttagccctaaacacaaataattacacaaacaaaattgttcaccagagtactagcggcaacagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddddddddddddddddc~`~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~a~s~~~v~~~~~~~~}~~a~~~~~~~dddddcbddddddcddddd +@HELIUM_000100422_612GNAAXX:7:76:18268:14980#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=66.9; seq_b_deletion=0; mid_quality=78.3208955224; seq_a_deletion=0; seq_b_single=46; score=239.985254914; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.8441558442; sminL=40.0; seq_a_single=46; score_norm=3.87072991797; seq_b_insertion=0; +ttacccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +d\ccdcddddddddddecd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~z~~~~~~~~~~~~~~~~~~~~b~~~~adbdbd^bcccddHddbdb +@HELIUM_000100422_612GNAAXX:7:16:15509:18069#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=58; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=4; tail_quality=66.8; seq_b_deletion=0; mid_quality=77.5; seq_a_deletion=0; seq_b_single=46; score=215.81634366; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.1233766234; sminL=40.0; seq_a_single=46; score_norm=3.48090876871; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +cdYdddddbdddbdcddda~~~~~~~~~~~~~~~~~~}a~r~~~~~~~~~~~~~~]zz~~~~}~~~~~~~~~~~~~b}}~_dddddddbdddddLaddbd +@HELIUM_000100422_612GNAAXX:7:25:7789:3090#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=3; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=72.4179104478; seq_a_deletion=0; seq_b_single=46; score=223.705772044; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.5; avg_quality=71.6818181818; sminL=40.0; seq_a_single=46; score_norm=3.60815761361; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactacaggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ead]dddddddddddddbd~~~~~~~~~~~~{~}~~~~cBBccccccccccccBccc~~z}~y~p~~~w|j||~~~uzr|tNba^\bT^cccZ\L\]`_` +@HELIUM_000100422_612GNAAXX:7:53:18419:8404#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.5447761194; seq_a_deletion=0; seq_b_single=46; score=231.841887233; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.0454545455; sminL=40.0; seq_a_single=46; score_norm=3.73938527796; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +decddddddddddddddddb~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~{~~~~b~~~~~~~~~~~~~~~{~~~~~~~~~ddddddedddddd_ddddd +@HELIUM_000100422_612GNAAXX:7:19:11039:3957#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.8432835821; seq_a_deletion=0; seq_b_single=46; score=247.811165598; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.7; avg_quality=77.2857142857; sminL=40.0; seq_a_single=46; score_norm=3.99695428384; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddcdddddddddddddad~~~~~~~~~~~~~~~~~~~~~~~~~~~}~~~~~~~~~~v~~~~~~~~~~~~~~~~~~~~~~~ddbdddbadddddbddddd +@HELIUM_000100422_612GNAAXX:7:42:19757:3800#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=57; sminR=40.0; seq_b_mismatch=3; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=1; mid_quality=77.7669172932; seq_a_deletion=1; seq_b_single=46; score=187.78301321; seq_a_insertion=1; mode=alignment; seq_length_ori=153; head_quality=67.1; avg_quality=76.3660130719; sminL=40.0; seq_a_single=45; score_norm=3.02875827758; seq_b_insertion=0; +ttagccctaaacacaagtaattaatatacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddedddeddddddcdcc~~~~~~~b~|~~~a~`~~~~~~~~~~~vz~~~~~~~~~~~x_~~~~~~~~~~~~~]~~~~~~dddddd`dddddadddcd +@HELIUM_000100422_612GNAAXX:7:116:14244:17926#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=55; sminR=40.0; seq_b_mismatch=3; seq_a_mismatch=5; tail_quality=67.0; seq_b_deletion=0; mid_quality=76.5338345865; seq_a_deletion=0; seq_b_single=45; score=187.882758912; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=75.2875816993; sminL=40.0; seq_a_single=45; score_norm=2.98226601448; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgcaagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +d`dbcccc`dddddeddd~~~~~~_~~~~b~~~~~~^xz{ZRzpooop~~s~~~~~~`~~~~~~~~~~~~~~~~~b~~~b~dddddedddddddddddd +@HELIUM_000100422_612GNAAXX:7:91:10071:8960#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.3233082707; seq_a_deletion=0; seq_b_single=45; score=251.979171474; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.7124183007; sminL=40.0; seq_a_single=45; score_norm=3.99966938848; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +ddddddddddddddeddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:61:17484:5085#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.9776119403; seq_a_deletion=0; seq_b_single=46; score=247.996979391; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.9; avg_quality=77.4155844156; sminL=40.0; seq_a_single=46; score_norm=3.9999512805; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddedddddddddddd`d~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddccddcddd +@HELIUM_000100422_612GNAAXX:7:18:2721:16829#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.3308270677; seq_a_deletion=0; seq_b_single=45; score=251.960802952; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.2; avg_quality=77.7320261438; sminL=40.0; seq_a_single=45; score_norm=3.99937782464; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddeeddddedddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dbdddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:2:7296:17275#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=2; seq_a_mismatch=1; tail_quality=66.9; seq_b_deletion=0; mid_quality=78.0298507463; seq_a_deletion=0; seq_b_single=46; score=223.923525795; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.3; avg_quality=76.5454545455; sminL=40.0; seq_a_single=46; score_norm=3.61166977089; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dbcacbcbd`ddddddd_b~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~vbdddddddddddcc]baddb +@HELIUM_000100422_612GNAAXX:7:58:8591:17028#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=2; tail_quality=66.0; seq_b_deletion=0; mid_quality=75.8582089552; seq_a_deletion=0; seq_b_single=46; score=223.788176649; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=65.3; avg_quality=74.5324675325; sminL=40.0; seq_a_single=46; score_norm=3.60948672015; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +_bcccccccbccacccccc~~~~~a~~y~~~~~wyn~~~~x~~zrzzkpppp~~~~~~{zuxx~~~~~z~xw~`~~_~~~~aaac]ca`cccccacc__[ +@HELIUM_000100422_612GNAAXX:7:13:3469:18630#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=58; sminR=40.0; seq_b_mismatch=2; seq_a_mismatch=3; tail_quality=66.0; seq_b_deletion=0; mid_quality=76.2631578947; seq_a_deletion=0; seq_b_single=45; score=211.840326012; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=66.3; avg_quality=74.9411764706; sminL=40.0; seq_a_single=45; score_norm=3.36254485733; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddIQQPPdadddcdadd~~~~~xyyLqV{w~~~~~~~~~~~~~~~~~~zszx{|yy{~~~~~~~~~~~~~~[~~b~~~b~ddddd_dddd^`a_cdd` +@HELIUM_000100422_612GNAAXX:7:72:12959:5736#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=56; sminR=40.0; seq_b_mismatch=7; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=63.5263157895; seq_a_deletion=0; seq_b_single=45; score=195.664863172; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=59.8; avg_quality=63.5098039216; sminL=40.0; seq_a_single=45; score_norm=3.10579147892; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacacccct ++ +^\bSbbbbddddd^dddd~~~~~~~~~~~~~~~b~~ib~~~~~~_~ccBBBcccccccccBccccccccccccccccccccDDDDDDDDDDDDDDDDDD +@HELIUM_000100422_612GNAAXX:7:115:6263:17700#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.6390977444; seq_a_deletion=0; seq_b_single=45; score=235.853550005; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.1176470588; sminL=40.0; seq_a_single=45; score_norm=3.74370714293; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +ddcdddddddddddeddd~~~~~~~~~~~~~~~~~~~~~~~~~~y|z{yxx~~{~~~~~~~~~~~~~~~~~~~~~b~~~b~ddddeddddddddddddd +@HELIUM_000100422_612GNAAXX:7:7:15538:8325#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=52; sminR=40.0; seq_b_mismatch=10; seq_a_mismatch=0; tail_quality=63.4; seq_b_deletion=1; mid_quality=60.1879699248; seq_a_deletion=0; seq_b_single=45; score=151.662770749; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=59.2; avg_quality=60.3333333333; sminL=40.0; seq_a_single=45; score_norm=2.40734556744; seq_b_insertion=1; +ttagccctaaacataaacattcaataaacaagaatgtcggccagagtactactagcaacagcatgaaactcaaagaactgggcggtgctttacatccct ++ +_S\abcacca_cacccc_~~y}~~~~qxYzgY^~~~accBccccccccccccccBBccccccBccccccBcccccBcccBcDDDDDDDDDDDDDDDDDD +@HELIUM_000100422_612GNAAXX:7:120:16005:7955#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=98; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=64; seq_ab_match=61; sminR=40.0; seq_b_mismatch=2; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.8939393939; seq_a_deletion=0; seq_b_single=44; score=231.880428082; seq_a_insertion=0; mode=alignment; seq_length_ori=152; head_quality=67.0; avg_quality=77.3289473684; sminL=40.0; seq_a_single=44; score_norm=3.62313168878; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagccgaaactcaaaggacttggcggtgctttacatccct ++ +ddddddddddddddddd~~~~b~~~~~~~~~b~~~~~~~~~~~z~~~~~~~~~~{~~~~~~~`~~~~~~~~~~~~~~~~~~ddddcdddddddddddd +@HELIUM_000100422_612GNAAXX:7:115:3396:14235#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=61; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.1052631579; seq_a_deletion=0; seq_b_single=45; score=235.745732131; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=76.6535947712; sminL=40.0; seq_a_single=45; score_norm=3.74199574811; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +ddcdddddadddddcddd~~~~~~b~~~~~~~~~}~}~ccc~|~~~~{~~~~~~~s~~~~~~~~~~~~~~~~~~~b~~~~~ddddddddddcdcddddd +@HELIUM_000100422_612GNAAXX:7:32:15197:9940#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=66.9; seq_b_deletion=0; mid_quality=78.3656716418; seq_a_deletion=0; seq_b_single=46; score=231.86950825; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.1; avg_quality=76.8896103896; sminL=40.0; seq_a_single=46; score_norm=3.73983077822; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddcaddddddddddddcdd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~wt}}~~~~~{~~~~~~~~~~~~~~~b~~~~~~bd`ddddcddddddcddddd +@HELIUM_000100422_612GNAAXX:7:7:17828:13825#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=56; sminR=40.0; seq_b_mismatch=3; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=1; mid_quality=77.5223880597; seq_a_deletion=0; seq_b_single=46; score=187.930205125; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.1558441558; sminL=40.0; seq_a_single=46; score_norm=3.03113234072; seq_b_insertion=1; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddbddddddddacbaa[b~b~~~~~~~~~~~~~a~~~~~~~~~~~~b~~~~~~~~~~~b~~~~~~~b}~~~~~~~~~~~_ddddddddddddccdddcd +@HELIUM_000100422_612GNAAXX:7:53:2128:13141#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=15a_F730814; experiment=wolf_diet; forward_tag=gaagtag; reverse_tag=gaagtag; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.947761194; seq_a_deletion=0; seq_b_single=46; score=247.978630411; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.9; avg_quality=77.3896103896; sminL=40.0; seq_a_single=46; score_norm=3.99965532921; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddddddddddddd^dad~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddcddddddddddcddddd +@HELIUM_000100422_612GNAAXX:7:72:7057:20448#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.0298507463; seq_a_deletion=0; seq_b_single=46; score=247.94833093; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.4675324675; sminL=40.0; seq_a_single=46; score_norm=3.9991666279; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddeddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddebdddd +@HELIUM_000100422_612GNAAXX:7:10:9237:10532#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=57; sminR=40.0; seq_b_mismatch=3; seq_a_mismatch=2; tail_quality=66.9; seq_b_deletion=0; mid_quality=75.9402985075; seq_a_deletion=0; seq_b_single=46; score=207.90944986; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.7; avg_quality=74.7532467532; sminL=40.0; seq_a_single=46; score_norm=3.35337822355; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaataattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +cSc`ddddddddddda_\`~~~~~~~~~~~~~~~~N~~\~~~~~~~~~~~~~~~Y~~jopppx|~|o~~~~~~b~~b~~~~ddddadddcadddada]V] +@HELIUM_000100422_612GNAAXX:7:16:16055:12835#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.7462686567; seq_a_deletion=0; seq_b_single=46; score=239.868244499; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.2207792208; sminL=40.0; seq_a_single=46; score_norm=3.86884265322; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +db`dddcdddddddddddc~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~dddddddddddddcddddd +@HELIUM_000100422_612GNAAXX:7:17:16877:9760#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=3; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.3731343284; seq_a_deletion=0; seq_b_single=46; score=223.899159093; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.8961038961; sminL=40.0; seq_a_single=46; score_norm=3.61127675956; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddddddddddcddddddd~b~~~~~~~~~~~~~a~~~~~~b~~~{~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:47:8320:8571#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=61; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.4586466165; seq_a_deletion=0; seq_b_single=45; score=235.809300513; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=66.9; avg_quality=76.954248366; sminL=40.0; seq_a_single=45; score_norm=3.74300477004; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddddddddddddddddd~~|~~~~~b~~~~~~~~~~~~~~~~~~~~~uuy~~~~~~~~~~~|~~~~~x~~~~~~~~~~b~daddddadddddd\ddda +@HELIUM_000100422_612GNAAXX:7:6:6617:2744#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.7593984962; seq_a_deletion=0; seq_b_single=45; score=235.875132744; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.2222222222; sminL=40.0; seq_a_single=45; score_norm=3.7440497261; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +eddddd`ddddddddcbd~b~~~~b~~~~~~~~~~~~|~~~~~~~~y~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddcbdd +@HELIUM_000100422_612GNAAXX:7:12:9609:14740#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.1; seq_b_deletion=0; mid_quality=78.4701492537; seq_a_deletion=0; seq_b_single=46; score=231.833538451; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.1; avg_quality=76.9935064935; sminL=40.0; seq_a_single=46; score_norm=3.73925062018; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dcddddddddddcdddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~zs~}~~~~~}~~~~~~~~~~~~~~~~~~b~~~bdddddddcdddddeddddd +@HELIUM_000100422_612GNAAXX:7:21:2093:4412#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=3; tail_quality=67.0; seq_b_deletion=0; mid_quality=76.1428571429; seq_a_deletion=0; seq_b_single=45; score=227.763131817; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=74.9477124183; sminL=40.0; seq_a_single=45; score_norm=3.61528780662; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +eddddddddddddddddd~b~~~~~~~~~~~~~}~~`~~~~~~~~~~~~~Z{~~|{~~~~{~|~|~~~~~}~~~}|~~}}}DDDDDDDD``U`^\[ddd +@HELIUM_000100422_612GNAAXX:7:18:5240:1587#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=2; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.3880597015; seq_a_deletion=0; seq_b_single=46; score=223.905608015; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.9090909091; sminL=40.0; seq_a_single=46; score_norm=3.61138077443; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddddddddddddddddd~}~~~~~~~~~~~~}~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~bdddddddedddddcddddd +@HELIUM_000100422_612GNAAXX:7:88:15797:19191#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=58; sminR=40.0; seq_b_mismatch=4; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.1492537313; seq_a_deletion=0; seq_b_single=46; score=215.88300628; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.7012987013; sminL=40.0; seq_a_single=46; score_norm=3.48198397226; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddddddddddddddcddc~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~`~~~~b~~b~~~~ddeddddddddddcddddd +@HELIUM_000100422_612GNAAXX:7:47:11864:1343#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.2857142857; seq_a_deletion=0; seq_b_single=45; score=251.976310174; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.6797385621; sminL=40.0; seq_a_single=45; score_norm=3.99962397102; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +ddddddddddddddbdcd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddcddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:43:8022:8427#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.5970149254; seq_a_deletion=0; seq_b_single=46; score=231.869859511; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.0909090909; sminL=40.0; seq_a_single=46; score_norm=3.73983644372; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dedcddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~b~~~~dddddddddddddcddddd +@HELIUM_000100422_612GNAAXX:7:95:11283:2557#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=4; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=58; seq_ab_match=57; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=0; tail_quality=93.0; seq_b_deletion=50; mid_quality=92.2368421053; seq_a_deletion=50; seq_b_single=0; score=223.999078974; seq_a_insertion=0; mode=alignment; seq_length_ori=58; head_quality=93.0; avg_quality=92.5; sminL=40.0; seq_a_single=0; score_norm=3.86205308576; seq_b_insertion=0; +tttt ++ +~~~~ +@HELIUM_000100422_612GNAAXX:7:67:5288:13403#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.0298507463; seq_a_deletion=0; seq_b_single=46; score=247.999078967; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.1; avg_quality=77.474025974; sminL=40.0; seq_a_single=46; score_norm=3.99998514464; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddddddddcdddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddeddddd +@HELIUM_000100422_612GNAAXX:7:7:11998:4462#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=59; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=4; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.2105263158; seq_a_deletion=0; seq_b_single=45; score=219.864332815; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=76.7450980392; sminL=40.0; seq_a_single=45; score_norm=3.48991004469; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtagtactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +ddddddddddddddcddd~b~~~~~~~~~~~~b~~~~~~~b~~~~~~~G~}}~~}}~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddcdccdd +@HELIUM_000100422_612GNAAXX:7:116:12598:9964#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=53; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=8; tail_quality=66.7; seq_b_deletion=0; mid_quality=77.0671641791; seq_a_deletion=0; seq_b_single=46; score=175.843125508; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=75.7402597403; sminL=40.0; seq_a_single=46; score_norm=2.83617944368; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddddcdddddddbddda`~~~~~~~~~~~~~~~~~~b~~~b~~~~b~~~~~b~b~{~~~~~~b~~~~~~~~~~~b~~~bddddddedddddddddddd +@HELIUM_000100422_612GNAAXX:7:111:18277:17779#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.8646616541; seq_a_deletion=0; seq_b_single=45; score=235.877819976; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.3137254902; sminL=40.0; seq_a_single=45; score_norm=3.74409238057; seq_b_insertion=0; +ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaatagcctgaaactcaaaggacttggcggtgctttacatccct ++ +ddddddddddddbdddcd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~_~~~~~~~~~~~~b~~~~~dddddddedddddddddd +@HELIUM_000100422_612GNAAXX:7:85:13257:14594#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.5298507463; seq_a_deletion=0; seq_b_single=46; score=231.92296471; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.0324675325; sminL=40.0; seq_a_single=46; score_norm=3.7406929792; seq_b_insertion=0; +ttagccctaaacacaaataattacacaaacaaaattgttcaccagagtactagcggcaacagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddddddedddddddddd~~~~~~~~~~~~~~~~~~~~~~~_~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~addccddadddddcaddddd +@HELIUM_000100422_612GNAAXX:7:17:11935:17343#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.0902255639; seq_a_deletion=0; seq_b_single=45; score=243.983570342; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.5098039216; sminL=40.0; seq_a_single=45; score_norm=3.87275508479; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddddddddddddddddd~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:38:9654:18700#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.0902255639; seq_a_deletion=0; seq_b_single=45; score=243.950053136; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.5098039216; sminL=40.0; seq_a_single=45; score_norm=3.87222306566; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +ddddddddddddddeddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~~~~~~~~~~~dcddddddddddcddddc +@HELIUM_000100422_612GNAAXX:7:101:11720:19906#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=57; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=4; tail_quality=66.9; seq_b_deletion=0; mid_quality=76.9104477612; seq_a_deletion=0; seq_b_single=46; score=207.841888471; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=75.6168831169; sminL=40.0; seq_a_single=46; score_norm=3.35228852373; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddc_ddddddcdddddd\d~~~~~~~~~~~~~~~~]~~|~hsp~~{~~z{t}~~qs}~b~~}~~~~}~x~~~~b~~c~~~_ddddcd^ccbbdd^ddddd +@HELIUM_000100422_612GNAAXX:7:42:12961:8650#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.1052631579; seq_a_deletion=0; seq_b_single=45; score=243.985409859; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.522875817; sminL=40.0; seq_a_single=45; score_norm=3.87278428347; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddddddddddddddddd~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddcddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:36:10388:10324#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=15a_F730814; experiment=wolf_diet; forward_tag=gaagtag; reverse_tag=gaagtag; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.8208955224; seq_a_deletion=0; seq_b_single=46; score=239.950587748; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.2857142857; sminL=40.0; seq_a_single=46; score_norm=3.87017077012; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddddddddddddddddd~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddeddddd +@HELIUM_000100422_612GNAAXX:7:66:11860:20419#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.1; seq_b_deletion=0; mid_quality=78.5671641791; seq_a_deletion=0; seq_b_single=46; score=239.911387587; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.1; avg_quality=77.0779220779; sminL=40.0; seq_a_single=46; score_norm=3.86953850946; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddcdddddeddddddddad~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~}~~~~~~~~~~~~~~~b~~~|ddcddddddddddNddddd +@HELIUM_000100422_612GNAAXX:7:3:17077:6562#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=3; tail_quality=66.6; seq_b_deletion=0; mid_quality=77.6567164179; seq_a_deletion=0; seq_b_single=46; score=223.876050008; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.0; avg_quality=76.1818181818; sminL=40.0; seq_a_single=46; score_norm=3.61090403239; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactgccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddcdddddddddddadab~~~~~~~~~~~~~~~~~~~~~~~~~~~~zz~zF~a~~~~~~~}~~~~~~~~~~~~~~au~~~ccccccccccccacccccc +@HELIUM_000100422_612GNAAXX:7:18:11865:6418#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.3157894737; seq_a_deletion=0; seq_b_single=45; score=251.959188225; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.7058823529; sminL=40.0; seq_a_single=45; score_norm=3.99935219405; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddcdddeddddddd +@HELIUM_000100422_612GNAAXX:7:19:4311:13343#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=58; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=4; tail_quality=67.0; seq_b_deletion=0; mid_quality=77.7969924812; seq_a_deletion=0; seq_b_single=45; score=211.837822462; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=76.385620915; sminL=40.0; seq_a_single=45; score_norm=3.36250511845; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactacgagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dbddcddddcdd^ddddd~~~~~~~~~~~~~b~~~~~~~~~~~~~~~~~~~{Z{~~~_~~y{~~~}~~~|~~~~~b~~~b~bcdddcdddddddddccc +@HELIUM_000100422_612GNAAXX:7:30:1800:7833#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=15a_F730814; experiment=wolf_diet; forward_tag=gaagtag; reverse_tag=gaagtag; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=52; sminR=40.0; seq_b_mismatch=10; seq_a_mismatch=0; tail_quality=65.6; seq_b_deletion=0; mid_quality=59.9104477612; seq_a_deletion=0; seq_b_single=46; score=167.712615339; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=35.0; avg_quality=58.6623376623; sminL=40.0; seq_a_single=46; score_norm=2.70504218289; seq_b_insertion=0; +ttagccctaaacacaagacattaatataacgagattaatcgacagagtactaccggctatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +DDDDDDDDDDDDDDDDDDDccBccccccccBcBccccBBccBcccccccc~u~v}[wJ~~Q|~~~~~~~~~~~~a~~~~t~Z^ccc`c]bbc_[V_[`[` +@HELIUM_000100422_612GNAAXX:7:16:19299:8684#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.5; seq_a_deletion=0; seq_b_single=46; score=231.845804746; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.0064935065; sminL=40.0; seq_a_single=46; score_norm=3.73944846365; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +cdccddddeddddddadac~~~~~~~~~~~~~~~~~~~~~~~~|~~~~~~~~~~~~~~~~~~~~~~~~`~~~~~~~b~~~~dddddddddddddcddddd +@HELIUM_000100422_612GNAAXX:7:54:14618:14353#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.6865671642; seq_a_deletion=0; seq_b_single=46; score=239.8562981; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.1688311688; sminL=40.0; seq_a_single=46; score_norm=3.86864996935; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddcedddddddddddddde~~~~~~~~~~~~~~~~~~~~~~~~~~~~~z}|z~~~~~y~~~~~~~~~~~~~~~~~~b~~~~ddddddcddddddcddddd +@HELIUM_000100422_612GNAAXX:7:108:9222:18258#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=2; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.5; seq_a_deletion=0; seq_b_single=46; score=231.857703869; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.2; avg_quality=77.0194805195; sminL=40.0; seq_a_single=46; score_norm=3.73964038498; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcagacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddccddddddddddddddd~~~~~~~~~~~~~~~~~~~v~~~~~}~}}~~~~~`~~~~~~~~~~b~~~~~~~~~~~~~~~~dcddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:74:4783:20479#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=77.9029850746; seq_a_deletion=0; seq_b_single=46; score=239.87314657; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.487012987; sminL=40.0; seq_a_single=46; score_norm=3.86892171887; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddaddddddbddddZd`d~b~~~~~~~~~~~~~~~~~}~~~~~~~~~~~~~~~|z~~~~~xq~~~}~r~~~~~~~~~~yqcbbddWdadddddWddcda +@HELIUM_000100422_612GNAAXX:7:38:3005:20881#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=3; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.5939849624; seq_a_deletion=0; seq_b_single=45; score=227.901935995; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.0784313725; sminL=40.0; seq_a_single=45; score_norm=3.61749104754; seq_b_insertion=0; +ttagccctaaacatgaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +ddddddddddddddaedd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~a~~~~~~~|~~~~~~b~~b~~}~~dbdddcdddddbdddddd +@HELIUM_000100422_612GNAAXX:7:4:10139:2389#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=66.5; seq_b_deletion=0; mid_quality=78.4962406015; seq_a_deletion=0; seq_b_single=45; score=251.802206044; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=66.9; avg_quality=76.954248366; sminL=40.0; seq_a_single=45; score_norm=3.99686041339; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +daddaddddbdddddddc~~~~~~~~~{~~~~~~~~zxy~~|~~~~ru~~~~~~~~~~~~~~~~~~{~~p~~~~~~~~~~~ddddddddddabaP`ddd +@HELIUM_000100422_612GNAAXX:7:75:9674:16966#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=4; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=58; seq_ab_match=57; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=0; tail_quality=93.0; seq_b_deletion=50; mid_quality=92.2631578947; seq_a_deletion=50; seq_b_single=0; score=224.000075421; seq_a_insertion=0; mode=alignment; seq_length_ori=58; head_quality=93.0; avg_quality=92.5172413793; sminL=40.0; seq_a_single=0; score_norm=3.86207026589; seq_b_insertion=0; +tttt ++ +~~~~ +@HELIUM_000100422_612GNAAXX:7:59:15367:8376#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=15a_F730814; experiment=wolf_diet; forward_tag=gaagtag; reverse_tag=gaagtag; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.0149253731; seq_a_deletion=0; seq_b_single=46; score=247.996374666; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.4545454545; sminL=40.0; seq_a_single=46; score_norm=3.99994152687; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddddddddddddddddad~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddeddddd +@HELIUM_000100422_612GNAAXX:7:29:1328:13185#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=59; sminR=40.0; seq_b_mismatch=4; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.3007518797; seq_a_deletion=0; seq_b_single=45; score=223.94591652; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=76.8235294118; sminL=40.0; seq_a_single=45; score_norm=3.55469708763; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddddddddddddedadd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~W[~~~~~~~~~~~~~~~~~~~~~~~b~~~b~dddcdddddddcdddddd +@HELIUM_000100422_612GNAAXX:7:30:16081:4486#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=62; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.0902255639; seq_a_deletion=0; seq_b_single=45; score=243.950667893; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.5098039216; sminL=40.0; seq_a_single=45; score_norm=3.87223282369; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddaddddddddddeddd~~~c~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddcddddddddddd +@HELIUM_000100422_612GNAAXX:7:104:10274:7999#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.2556390977; seq_a_deletion=0; seq_b_single=45; score=251.962499638; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=66.9; avg_quality=77.6470588235; sminL=40.0; seq_a_single=45; score_norm=3.99940475616; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +ccdddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddd^ddddddddddd +@HELIUM_000100422_612GNAAXX:7:14:7706:12338#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=77.6194029851; seq_a_deletion=0; seq_b_single=46; score=223.864093999; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.7; avg_quality=76.2207792208; sminL=40.0; seq_a_single=46; score_norm=3.61071119354; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddadddcddddddddc^d~~~~~~~~~~~~~~~~~~~P~~~~~~~~~}}~vm~qZyw~~~~x~~~~~~~~~~~b~~~~~~dddddaccdddddcddddd diff -r 000000000000 -r aca6a8c9cf83 test-data/output_ngsfilter_error_3.fastq --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/output_ngsfilter_error_3.fastq Wed Apr 12 17:38:00 2017 -0400 @@ -0,0 +1,952 @@ +@HELIUM_000100422_612GNAAXX:7:119:14871:19157#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=47; sminR=40.0; seq_b_mismatch=13; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=1; mid_quality=69.1492537313; seq_a_deletion=0; seq_b_single=46; score=115.761290673; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=68.8701298701; sminL=40.0; seq_a_single=46; score_norm=1.86711759151; seq_b_insertion=1; +ttagccctaaacacaagtaattattataacaaaatcattcgccagagtactaccggcaatagctcaaaactcaaaggacttggcggtgctttataccctt ++ +cacdddddddddddddc\d~b~~~b~~~~~~b`ryK~|uxyXk`}~ccBBccBcccBcBcccBcBccccccc~~~~b|~~xdbaddaaWcccdaadddda +@HELIUM_000100422_612GNAAXX:7:108:5640:3823#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=2; seq_a_mismatch=0; tail_quality=66.9; seq_b_deletion=0; mid_quality=78.447761194; seq_a_deletion=0; seq_b_single=46; score=231.881364714; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.9545454545; sminL=40.0; seq_a_single=46; score_norm=3.74002201152; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattgttcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddcdddddcddddddd]d~b~~~~~~~~~~~~~Z~~~~~~~~~~~~~~~~~~~~~~~~~~~}~~~~~~~~~~~~~~~~~~ddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:97:14311:19299#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=52; sminR=40.0; seq_b_mismatch=5; seq_a_mismatch=5; tail_quality=67.0; seq_b_deletion=0; mid_quality=75.0820895522; seq_a_deletion=0; seq_b_single=46; score=167.91734192; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.6; avg_quality=74.0064935065; sminL=40.0; seq_a_single=46; score_norm=2.70834422451; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaagagcttaaaactcaaaggacttggcggtgctttataccctt ++ +d`cdddddcd`ddddaa\b~`~~~~~~~~b~~~~]~b~V~~~~~~~~~~~~~~~~`~~^Zs~r|~~~st~|z|v||^~~|TY``accL]`\`daZddddd +@HELIUM_000100422_612GNAAXX:7:22:8540:14708#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=3; seq_a_mismatch=0; tail_quality=66.9; seq_b_deletion=0; mid_quality=78.2537313433; seq_a_deletion=0; seq_b_single=46; score=223.982011431; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.1; avg_quality=76.7922077922; sminL=40.0; seq_a_single=46; score_norm=3.61261308759; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +addbddddddddddddedd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~b~~~~~~~~b~~~~ddddddddddabbb^bddd +@HELIUM_000100422_612GNAAXX:7:57:18459:16145#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=3; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.6917293233; seq_a_deletion=0; seq_b_single=45; score=227.883189945; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=66.9; avg_quality=77.1568627451; sminL=40.0; seq_a_single=45; score_norm=3.61719349119; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~c~~~~~~~~~~~~~~b~~~~~~b~dddddcdddddddddddd +@HELIUM_000100422_612GNAAXX:7:89:10281:5930#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.5373134328; seq_a_deletion=0; seq_b_single=46; score=231.893859399; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.038961039; sminL=40.0; seq_a_single=46; score_norm=3.7402235387; seq_b_insertion=0; +ttagccctaaacacaaataattacacaaacaaaattgttcaccagagtactagcggcaacagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddddddcddddddcdddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~bddddddceddedd\ddddd +@HELIUM_000100422_612GNAAXX:7:29:15520:18035#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=77.828358209; seq_a_deletion=0; seq_b_single=46; score=239.965360923; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=65.6; avg_quality=76.3311688312; sminL=40.0; seq_a_single=46; score_norm=3.87040904714; seq_b_insertion=0; +ttagccctaaacacaagtaattattataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +__`_Y_`_a_`cc_ccccc~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~ddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:6:9274:14951#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=2; seq_a_mismatch=1; tail_quality=67.1; seq_b_deletion=0; mid_quality=78.2089552239; seq_a_deletion=0; seq_b_single=46; score=223.791171196; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.9; avg_quality=76.7532467532; sminL=40.0; seq_a_single=46; score_norm=3.60953501929; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddcddddddddddddddd~~~~~~~~~~~~~~~~~~|z~}a|~~~~}}}}~~~~~~~~~~}~}b~b~~~~~~~~~~~~~~ddddddcdcddcd^ddddd +@HELIUM_000100422_612GNAAXX:7:111:2893:6772#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.7164179104; seq_a_deletion=0; seq_b_single=46; score=239.959941063; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.1948051948; sminL=40.0; seq_a_single=46; score_norm=3.87032163005; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddddddedddddded_d~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~x~dddddddcdddcdcdddad +@HELIUM_000100422_612GNAAXX:7:89:10621:20317#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=3; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=77.5820895522; seq_a_deletion=0; seq_b_single=46; score=223.827654468; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.2077922078; sminL=40.0; seq_a_single=46; score_norm=3.61012345916; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dd`ddddadadddddddRc~}~~z~~~~~~~t~sw}~~g}~~~~~~~~~~~~~~~~~~~~~~~~~~~~^~b`~~~~~~~~~ddddddddbdbdd_dddad +@HELIUM_000100422_612GNAAXX:7:50:2791:11138#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.3805970149; seq_a_deletion=0; seq_b_single=46; score=231.976017167; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.9; avg_quality=76.8961038961; sminL=40.0; seq_a_single=46; score_norm=3.74154866398; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dbdbdddddddc\ddddbd~~~~~~~~~~~~~~~~~~b~~~^~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddbddddd +@HELIUM_000100422_612GNAAXX:7:108:17701:18887#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=58; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=4; tail_quality=66.9; seq_b_deletion=0; mid_quality=77.6492537313; seq_a_deletion=0; seq_b_single=46; score=215.828321765; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.1; avg_quality=76.2012987013; sminL=40.0; seq_a_single=46; score_norm=3.48110196396; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddec`dddddddddddc^c~~~~~~~~~~~~~~xss~~~~~~~~~~~|{|z{~~{~~~b~~~~~b~~~~~~~~~~~b~~~bdddddd\ddddddcddddd +@HELIUM_000100422_612GNAAXX:7:97:10414:9929#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.8134328358; seq_a_deletion=0; seq_b_single=46; score=239.864541102; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.2792207792; sminL=40.0; seq_a_single=46; score_norm=3.86878292101; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddddddddddddedddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~ddddddcddddddcddddd +@HELIUM_000100422_612GNAAXX:7:97:9091:15775#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=3; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.276119403; seq_a_deletion=0; seq_b_single=46; score=223.861916171; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.8116883117; sminL=40.0; seq_a_single=46; score_norm=3.61067606728; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dcdaddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~x~~~~~~~~~|~~~~~~~b~~~~~~^~~b~~~~dddddddddddddcddddd +@HELIUM_000100422_612GNAAXX:7:74:8683:20738#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=62; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.0977443609; seq_a_deletion=0; seq_b_single=45; score=243.961428864; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.5163398693; sminL=40.0; seq_a_single=45; score_norm=3.87240363276; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~`~~~~~~~~~~~~~~ddeddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:24:16230:12703#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=77.4552238806; seq_a_deletion=0; seq_b_single=46; score=223.86908915; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.3; avg_quality=76.0519480519; sminL=40.0; seq_a_single=46; score_norm=3.61079176049; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatcccctt ++ +bdd_ddddaddcbdcdd^b~~~~~b~~~~~~~~~~~~~~~~~~~~~~~~u~y~~~~~~a~~~~~~~~~~~~~~~~{bs~}}a`ddd`a_ddd``Ob`cdd +@HELIUM_000100422_612GNAAXX:7:4:4214:9434#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=58; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=3; tail_quality=63.3; seq_b_deletion=0; mid_quality=76.2388059701; seq_a_deletion=0; seq_b_single=46; score=215.812703412; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=65.3; avg_quality=74.6883116883; sminL=40.0; seq_a_single=46; score_norm=3.48085005502; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactacaggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddbdddddcddddddddd~^~~~{~{~~~~~}~~{~~ym~L||~~wuy~zyxPzjyyvxku~~~~~~~~~~~~~~~~~xbacdddddaddac_^`_`dc +@HELIUM_000100422_612GNAAXX:7:12:17693:3451#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.8507462687; seq_a_deletion=0; seq_b_single=46; score=247.862243; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.3116883117; sminL=40.0; seq_a_single=46; score_norm=3.9977781129; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dedcddddddddddedddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~}v{}~{~}~~~~~~~~~~~~~~~~~~~~~~~~~cdddddcddddddaddddd +@HELIUM_000100422_612GNAAXX:7:3:14624:12375#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=61; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.7669172932; seq_a_deletion=0; seq_b_single=45; score=235.922125108; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.2287581699; sminL=40.0; seq_a_single=45; score_norm=3.74479563663; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dcdcdddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~Y`~~~~~~~~~~~~~~~~~~~~~~~dddddcdddddddceddd +@HELIUM_000100422_612GNAAXX:7:18:5317:11571#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.7462686567; seq_a_deletion=0; seq_b_single=46; score=239.862078449; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.2207792208; sminL=40.0; seq_a_single=46; score_norm=3.86874320079; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddddddddddddeddde~~~~~~~~~~~~~~~~~~~~~~~~~~~~y~~~~~~~~~~~~~~~~~{~~~~~~~~~~b~~~~dddddcdddddddaddddd +@HELIUM_000100422_612GNAAXX:7:109:17335:11429#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=56; sminR=40.0; seq_b_mismatch=6; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=73.1278195489; seq_a_deletion=0; seq_b_single=45; score=195.716358124; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=66.9; avg_quality=72.3202614379; sminL=40.0; seq_a_single=45; score_norm=3.10660885911; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddcdaddddddddcdbd~b~~~~~~~~~|~``}~\`~|[~ccccccccccccccccc~z~~s~x~~~~~}~~}}W~~}x~ddddc]``]\Rd`ddb`^ +@HELIUM_000100422_612GNAAXX:7:7:10646:8487#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=77.671641791; seq_a_deletion=0; seq_b_single=46; score=231.85443709; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.2857142857; sminL=40.0; seq_a_single=46; score_norm=3.739587695; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dccdddddddddddddddec~~~~~~~~~~~~~~~~~~~~~~~~~~~{vztippoph~~~~~~~b~~~~~~~~~~~~~~~~ddddddddddddddddddc +@HELIUM_000100422_612GNAAXX:7:19:4865:20715#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.5074626866; seq_a_deletion=0; seq_b_single=46; score=231.923942025; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.012987013; sminL=40.0; seq_a_single=46; score_norm=3.74070874234; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddcbddddddddddddddb~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~ybddddddddddcddadddcd +@HELIUM_000100422_612GNAAXX:7:13:6954:13039#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.0; seq_a_deletion=0; seq_b_single=45; score=243.872474161; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.431372549; sminL=40.0; seq_a_single=45; score_norm=3.87099165336; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagaggactactagcaatagcttaaaactcaaaggacttggcggtgctttatatccct ++ +dddcdddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~r~~~~~~~~~~~~~~~~~~~~~~~b~~~~~dddddddddddddcdddd +@HELIUM_000100422_612GNAAXX:7:120:14963:1929#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.1492537313; seq_a_deletion=0; seq_b_single=46; score=223.810433242; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.7012987013; sminL=40.0; seq_a_single=46; score_norm=3.60984569745; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddadddddddedddddbd~b~~}~~~~~~~~~~~~~~~~u~~|~~}~~}~~~}z`}}~~~}~~~~~~~~~~~~~~_~~~~dddddddddddddcddddd +@HELIUM_000100422_612GNAAXX:7:115:3921:10649#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=61; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.5864661654; seq_a_deletion=0; seq_b_single=45; score=235.871015095; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.0718954248; sminL=40.0; seq_a_single=45; score_norm=3.74398436659; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +ddedddddddddcddddd~~~~~~~~~~~~~b~~~~~~~~~~~~~~~~~}~}~n{~i~~~~~~~~~~~~~~~~~~~~~~b~dddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:109:12848:8882#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.3805970149; seq_a_deletion=0; seq_b_single=46; score=231.869965661; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.9025974026; sminL=40.0; seq_a_single=46; score_norm=3.73983815582; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddeedddddddddddcd`d~~~~~~~~~~~~~~~~~~~~~~~~~~~~~{~~x~b~~~}~~~~~~~~~~~~~~~~~~~~~~bdcdddddddddddTddddd +@HELIUM_000100422_612GNAAXX:7:84:11257:18886#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=55; sminR=40.0; seq_b_mismatch=8; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=77.1804511278; seq_a_deletion=0; seq_b_single=45; score=187.890540948; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=75.8496732026; sminL=40.0; seq_a_single=45; score_norm=2.98238953885; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddcdddddddddddddd~b~a~~b~~~~b~~~~~~{b~|a~~`\}s~y~~~~~~t~~~~~~~~~~~~~~~~~~~~x~~t~dddddddddddddddddc +@HELIUM_000100422_612GNAAXX:7:72:13060:5051#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.671641791; seq_a_deletion=0; seq_b_single=46; score=239.985823237; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.8; avg_quality=77.1428571429; sminL=40.0; seq_a_single=46; score_norm=3.87073908447; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddddddddddddddd_b~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddeddddd +@HELIUM_000100422_612GNAAXX:7:35:3798:1896#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=15a_F730814; experiment=wolf_diet; forward_tag=gaagtag; reverse_tag=gaagtag; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=66.9; seq_b_deletion=0; mid_quality=78.4701492537; seq_a_deletion=0; seq_b_single=46; score=239.863223783; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.974025974; sminL=40.0; seq_a_single=46; score_norm=3.86876167392; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddddddcddbddddddad~b~~}~~~~~~~~~~~~~~~~~n~~~~~|~}~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddcddaddaddd^dddbc +@HELIUM_000100422_612GNAAXX:7:117:12470:18434#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=59; sminR=40.0; seq_b_mismatch=4; seq_a_mismatch=0; tail_quality=66.9; seq_b_deletion=0; mid_quality=78.045112782; seq_a_deletion=0; seq_b_single=45; score=219.83660627; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=66.9; avg_quality=76.5882352941; sminL=40.0; seq_a_single=45; score_norm=3.4894699408; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddddddddcddddddcd~~~~~~~~~~~~~~~~~~~~~~~~~~~~O~~~~~~~~~{~~{~~Wx~~~~~~~~~~~b~~~b}ddabdddddddddddddd +@HELIUM_000100422_612GNAAXX:7:11:8428:13716#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=15a_F730814; experiment=wolf_diet; forward_tag=gaagtag; reverse_tag=gaagtag; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.7985074627; seq_a_deletion=0; seq_b_single=46; score=239.97387484; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.2662337662; sminL=40.0; seq_a_single=46; score_norm=3.87054636839; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddddddddddddddddad~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:78:18994:16521#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.6940298507; seq_a_deletion=0; seq_b_single=46; score=239.927346216; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.8; avg_quality=77.1623376623; sminL=40.0; seq_a_single=46; score_norm=3.86979590672; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +cddddddcdddddddddad~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~bddaddeeaddded`ddddb +@HELIUM_000100422_612GNAAXX:7:16:5047:7843#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.3007518797; seq_a_deletion=0; seq_b_single=45; score=251.979285107; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.6928104575; sminL=40.0; seq_a_single=45; score_norm=3.99967119217; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dedddbdddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddcdcddddddeec +@HELIUM_000100422_612GNAAXX:7:118:13803:15594#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=60; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.5112781955; seq_a_deletion=0; seq_b_single=45; score=227.87720055; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.0065359477; sminL=40.0; seq_a_single=45; score_norm=3.61709842144; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +edd^ddddbdddddeddd~~~~~~~~~~~~~~~~~~a~~~~~~~~~~~{~y~~~~~~~~~~v~~~~~~~~~~b~~b~~~~~dddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:53:14379:5922#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.0; seq_a_deletion=0; seq_b_single=46; score=247.982071703; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.9; avg_quality=77.4350649351; sminL=40.0; seq_a_single=46; score_norm=3.99971083392; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddddddddddddddd`d~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddeddddd +@HELIUM_000100422_612GNAAXX:7:52:10700:16508#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=64.5; seq_b_deletion=0; mid_quality=77.8805970149; seq_a_deletion=0; seq_b_single=46; score=239.790995416; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.3051948052; sminL=40.0; seq_a_single=46; score_norm=3.86759670025; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +c^ccccccccccacccccc~~~~~~~~~~~~~~{{~~v~~~~~~~~~~~~~~~~}}}~~~~~{~~~~|v|||~}~~~u~~bddddcdbdddbddIddddd +@HELIUM_000100422_612GNAAXX:7:69:7696:12881#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.3082706767; seq_a_deletion=0; seq_b_single=45; score=251.983052061; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.6993464052; sminL=40.0; seq_a_single=45; score_norm=3.99973098509; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +eddddddcdddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddcddd +@HELIUM_000100422_612GNAAXX:7:44:12484:5320#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.1; seq_b_deletion=0; mid_quality=78.5149253731; seq_a_deletion=0; seq_b_single=46; score=231.837799589; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.7; avg_quality=77.0064935065; sminL=40.0; seq_a_single=46; score_norm=3.73931934821; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +deddddddddddddddddc~~~~~~~~~~~~~~~~~~~}}}z~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~adcddddadddddddddddd +@HELIUM_000100422_612GNAAXX:7:21:4713:10229#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.7313432836; seq_a_deletion=0; seq_b_single=46; score=239.864510201; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.2077922078; sminL=40.0; seq_a_single=46; score_norm=3.86878242259; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dcdbddddddddcdddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~|~~~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~~ddddddddddddd`ddddb +@HELIUM_000100422_612GNAAXX:7:38:6201:12003#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=59; sminR=40.0; seq_b_mismatch=4; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=67.7443609023; seq_a_deletion=0; seq_b_single=45; score=219.686655506; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=63.2; avg_quality=67.3986928105; sminL=40.0; seq_a_single=45; score_norm=3.48708976993; seq_b_insertion=0; +ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaatagcttaaaactcaaagaactgggcggtgctttatatcccg ++ +dbddcddcddd[dddddc~~~~b~~~~~~~t~}{axzyz{||zzwzr~y}}}}}z~x~cccccccccccccccccBcccBcDDDDDDDDDDDDDDDDaH +@HELIUM_000100422_612GNAAXX:7:33:16506:1490#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.2; seq_b_deletion=0; mid_quality=79.0373134328; seq_a_deletion=0; seq_b_single=46; score=247.963002972; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.1; avg_quality=77.4935064935; sminL=40.0; seq_a_single=46; score_norm=3.99940327375; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddeddddd +@HELIUM_000100422_612GNAAXX:7:32:4360:3451#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=61; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.7703703704; seq_a_deletion=0; seq_b_single=47; score=243.999335649; seq_a_insertion=0; mode=alignment; seq_length_ori=155; head_quality=67.0; avg_quality=77.2516129032; sminL=40.0; seq_a_single=47; score_norm=3.999989109; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddddddddddddddddde~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddeddddddddddeedddd +@HELIUM_000100422_612GNAAXX:7:115:17402:4174#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=3; tail_quality=67.1; seq_b_deletion=0; mid_quality=77.9402985075; seq_a_deletion=0; seq_b_single=46; score=223.877240886; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.525974026; sminL=40.0; seq_a_single=46; score_norm=3.6109232401; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactacctgcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +edddddccddddedcdd]d~~~~~~~~~~~~~~~~~~~~~~~~~~~~~s~|y~vS~~~~~~{~~~~~~~~~~~~~~b~~~bdddddddddddddcddddd +@HELIUM_000100422_612GNAAXX:7:45:6831:6228#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.0298507463; seq_a_deletion=0; seq_b_single=46; score=247.974637619; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.4675324675; sminL=40.0; seq_a_single=46; score_norm=3.99959092934; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddcddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddeddddd +@HELIUM_000100422_612GNAAXX:7:117:4291:18111#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=62; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.0827067669; seq_a_deletion=0; seq_b_single=45; score=243.911625561; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.5032679739; sminL=40.0; seq_a_single=45; score_norm=3.87161310415; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +ddddddddaddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~dddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:71:14800:3222#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.5597014925; seq_a_deletion=0; seq_b_single=46; score=231.89389474; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.0584415584; sminL=40.0; seq_a_single=46; score_norm=3.74022410871; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddddddeeddddddddcd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~}~~~\~~~~~~~~~~~~~~~~~b~~~~ddddddccdddddcdddde +@HELIUM_000100422_612GNAAXX:7:11:8547:18238#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.2932330827; seq_a_deletion=0; seq_b_single=45; score=251.984413754; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.6862745098; sminL=40.0; seq_a_single=45; score_norm=3.99975259927; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dbdcdddddddddcdddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:117:2416:10342#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.2781954887; seq_a_deletion=0; seq_b_single=45; score=235.921215944; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=76.8039215686; sminL=40.0; seq_a_single=45; score_norm=3.74478120546; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +d^dUYZ[Yddddcdcddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~}~~~~~b|~~b~dddddbddddbddddddd +@HELIUM_000100422_612GNAAXX:7:78:7151:19928#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=56; sminR=40.0; seq_b_mismatch=7; seq_a_mismatch=0; tail_quality=47.6; seq_b_deletion=0; mid_quality=58.2105263158; seq_a_deletion=0; seq_b_single=45; score=195.576462373; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=52.9; avg_quality=57.1699346405; sminL=40.0; seq_a_single=45; score_norm=3.10438829164; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacgagaatgttcgccagtgtacttctagcaacaggctgaaactcaaaggacttggcggtggtttacatccct ++ +WSR]YbbbDDDDDDDDDDcccccccccccBBcccccccccccccBcccccBccccccccccBccccccccccBccccc~`~WNS]Y_KTUVZ[O_SHW] +@HELIUM_000100422_612GNAAXX:7:71:18821:20471#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=53; sminR=40.0; seq_b_mismatch=2; seq_a_mismatch=7; tail_quality=66.9; seq_b_deletion=0; mid_quality=75.9029850746; seq_a_deletion=0; seq_b_single=46; score=175.889514148; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=74.7402597403; sminL=40.0; seq_a_single=46; score_norm=2.83692764755; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dd\ddddddddd^ddd`dd~~~~Xx~~~~~{|q}}x}^Y`J`~Xv{wt~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~sbddddddddddddd^bdccd +@HELIUM_000100422_612GNAAXX:7:58:15837:8531#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.2857142857; seq_a_deletion=0; seq_b_single=45; score=251.96128051; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.6797385621; sminL=40.0; seq_a_single=45; score_norm=3.99938540492; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddbdddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddcddddd +@HELIUM_000100422_612GNAAXX:7:38:10813:16867#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.2406015038; seq_a_deletion=0; seq_b_single=45; score=251.97777927; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.6405228758; sminL=40.0; seq_a_single=45; score_norm=3.99964729; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgtttgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +ddddddddeddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dbcdddddadadcddddd +@HELIUM_000100422_612GNAAXX:7:68:19115:18847#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=54; sminR=40.0; seq_b_mismatch=2; seq_a_mismatch=6; tail_quality=66.6; seq_b_deletion=0; mid_quality=76.0074626866; seq_a_deletion=0; seq_b_single=46; score=183.856571062; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.7; avg_quality=74.7922077922; sminL=40.0; seq_a_single=46; score_norm=2.96542856552; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddecdd_ddcdd_dd^ddd~`~~~~~b~~~~~~~~~~~}~~{b~~~~~~~{~~_~~~s~~s~~~~x~~\\~~~~~~b~~{bd`dcc`P^bb^dbaaaddb +@HELIUM_000100422_612GNAAXX:7:14:3978:11028#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=77.9029850746; seq_a_deletion=0; seq_b_single=46; score=231.869517243; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.8; avg_quality=76.474025974; sminL=40.0; seq_a_single=46; score_norm=3.73983092328; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactacctgcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddeadbdcddddeddd`ad~~~~~~~~~~~~~~~~~~~~~~~~~~~~~w|{u~|J~y~~~v~~~~~~~~~~~~~~~~~~~_dddddddddddbbZ`bcdd +@HELIUM_000100422_612GNAAXX:7:34:18733:3827#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.1052631579; seq_a_deletion=0; seq_b_single=45; score=243.983236898; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.522875817; sminL=40.0; seq_a_single=45; score_norm=3.87274979204; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddddddddddddddddd~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:65:6787:8456#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.5895522388; seq_a_deletion=0; seq_b_single=46; score=231.869006954; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.8; avg_quality=77.0714285714; sminL=40.0; seq_a_single=46; score_norm=3.7398226928; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddddddcddddddedd_d~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~bddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:111:7441:14688#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.015037594; seq_a_deletion=0; seq_b_single=45; score=243.832560428; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.4444444444; sminL=40.0; seq_a_single=45; score_norm=3.87035810203; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddadddddddddedddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~}~~~~~~~~~~~~~~~~~~u~~~~~b~~~~~dddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:86:10092:15563#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=2; tail_quality=66.8; seq_b_deletion=0; mid_quality=78.1940298507; seq_a_deletion=0; seq_b_single=46; score=223.927744132; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.6; avg_quality=76.7012987013; sminL=40.0; seq_a_single=46; score_norm=3.61173780858; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddbdddddccdddddaddd~b~~~~~~~~~~~~~_~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~|bddddddddddcddcddd`^ +@HELIUM_000100422_612GNAAXX:7:16:4418:5128#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=3; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.3656716418; seq_a_deletion=0; seq_b_single=46; score=223.862543287; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.8896103896; sminL=40.0; seq_a_single=46; score_norm=3.61068618206; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddeddddddddddddddb~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~{~~~~~~~~~~~~~~~b~~b~~~bdddeddbdddddddddddd +@HELIUM_000100422_612GNAAXX:7:13:5742:9859#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=4; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=58; seq_ab_match=55; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=3; tail_quality=93.0; seq_b_deletion=50; mid_quality=91.4210526316; seq_a_deletion=50; seq_b_single=0; score=207.846895043; seq_a_insertion=0; mode=alignment; seq_length_ori=58; head_quality=89.4; avg_quality=91.3448275862; sminL=40.0; seq_a_single=0; score_norm=3.58356715591; seq_b_insertion=0; +tttt ++ +~~~~ +@HELIUM_000100422_612GNAAXX:7:85:18986:17662#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=15a_F730814; experiment=wolf_diet; forward_tag=gaagtag; reverse_tag=gaagtag; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=2; seq_a_mismatch=0; tail_quality=67.1; seq_b_deletion=0; mid_quality=78.447761194; seq_a_deletion=0; seq_b_single=46; score=231.874929984; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.9; avg_quality=76.961038961; sminL=40.0; seq_a_single=46; score_norm=3.73991822554; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~}~~s~~~~~b~~~~~~b~~~~~~~ddcddddddcddddddddd +@HELIUM_000100422_612GNAAXX:7:16:16357:19948#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=57; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=5; tail_quality=66.7; seq_b_deletion=0; mid_quality=75.6541353383; seq_a_deletion=0; seq_b_single=45; score=203.860719115; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=62.0; avg_quality=74.1764705882; sminL=40.0; seq_a_single=45; score_norm=3.23588443039; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcacaggacttggcggtgctttacatccct ++ +dd_dbddbdaddbddddd~`~~b~~~~~~b~~{~~~}x}}~}`twyhw~~~~}w|~|~~~~~X~~~|~~~~~Y~~~~~~~~XVRSQ[Qadd``^P^adY +@HELIUM_000100422_612GNAAXX:7:107:3570:18147#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.0820895522; seq_a_deletion=0; seq_b_single=46; score=231.924768153; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=65.9; avg_quality=76.5714285714; sminL=40.0; seq_a_single=46; score_norm=3.74072206698; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcataaaactcaaaggacttggcggtgctttataccctt ++ +dddadddddcddddd\d\d~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~}~~~~~~~~a~s~`ddcd_ddcddddd]ddc_b +@HELIUM_000100422_612GNAAXX:7:82:8836:11354#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.2835820896; seq_a_deletion=0; seq_b_single=46; score=231.781218203; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.8181818182; sminL=40.0; seq_a_single=46; score_norm=3.73840674522; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddedddddddddddddddd~~~~~~{~~~~~~~~~~~~~~~~~~~~~~w{{k~~|~~~~~~~~~~~~~~~~~~~~~b~~~bddddddacdddddcdddda +@HELIUM_000100422_612GNAAXX:7:41:2354:1449#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=55; sminR=40.0; seq_b_mismatch=3; seq_a_mismatch=5; tail_quality=66.4; seq_b_deletion=0; mid_quality=75.5789473684; seq_a_deletion=0; seq_b_single=45; score=187.777927438; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=66.6; avg_quality=74.3921568627; sminL=40.0; seq_a_single=45; score_norm=2.98060202283; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +daaddddddcddbcdddd~b}bb~b~~~b~q~~}~}~x~x~t~s~|k|~w~~z~~{|{~ccBc~~}~~~~~~a~~a~}p~~baceddbbedddda_aa_ +@HELIUM_000100422_612GNAAXX:7:114:4511:17596#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=57; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=4; tail_quality=67.0; seq_b_deletion=0; mid_quality=75.3208955224; seq_a_deletion=0; seq_b_single=46; score=207.883730153; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.2; avg_quality=74.2532467532; sminL=40.0; seq_a_single=46; score_norm=3.35296338956; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtgctaccggcaatagcttaaaactcaaaggacttggcggtgctttatacgctt ++ +ddddddddddddddd^d^d~~~~~~~~~~~~~~~X~~~~~~~~~~~~~W}~~~~~a~sa~~~~~~~~~~~~~~~~~b~~~~ddddddQPPPPPQPKKPPP +@HELIUM_000100422_612GNAAXX:7:94:12367:17729#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=3; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.0; seq_a_deletion=0; seq_b_single=46; score=223.888596169; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.5714285714; sminL=40.0; seq_a_single=46; score_norm=3.61110638983; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddd`ddddddddddeddad~~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~~r|~t||x~~~~~~~~~~~~~~~~~}b~~~bcddddd`dbdadccddecc +@HELIUM_000100422_612GNAAXX:7:30:5374:18213#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.3233082707; seq_a_deletion=0; seq_b_single=45; score=251.958026467; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.7124183007; sminL=40.0; seq_a_single=45; score_norm=3.99933375344; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:49:11740:5547#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=62; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=0; tail_quality=66.6; seq_b_deletion=0; mid_quality=79.1127819549; seq_a_deletion=0; seq_b_single=45; score=243.959827817; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=66.6; avg_quality=77.477124183; sminL=40.0; seq_a_single=45; score_norm=3.87237821931; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +eddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~cddddddddcdddddddd +@HELIUM_000100422_612GNAAXX:7:29:3531:8314#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.8571428571; seq_a_deletion=0; seq_b_single=45; score=235.837856208; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.3071895425; sminL=40.0; seq_a_single=45; score_norm=3.74345803504; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +ddddddddddddddecdd~~~~~~~~~~~~~~~~~~~~~}~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~a~dcddddddddddcddddd +@HELIUM_000100422_612GNAAXX:7:114:10615:11217#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=1; tail_quality=66.9; seq_b_deletion=0; mid_quality=73.7985074627; seq_a_deletion=0; seq_b_single=46; score=231.702876359; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.7; avg_quality=72.8896103896; sminL=40.0; seq_a_single=46; score_norm=3.73714316708; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddddaaa``a_adddd^d~b~~z~~~~~~~~~~~~~cBcccccccccccccc~y~~s~~~~~~~|~~~~~|~~~~sz~~~adddddd[dad`VRW]VZd +@HELIUM_000100422_612GNAAXX:7:108:2729:8600#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.2462686567; seq_a_deletion=0; seq_b_single=46; score=223.856778095; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.7857142857; sminL=40.0; seq_a_single=46; score_norm=3.61059319508; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddddddddddedcdcad~~~~~~~~~~~~~~~a~~~~~~~~~~~~~~~~~~~~~~~`~~~~~~~~~~~}~~~~~b~~~~dddddddcddddWdddddd +@HELIUM_000100422_612GNAAXX:7:103:8847:6326#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.3082706767; seq_a_deletion=0; seq_b_single=45; score=251.968264217; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.6993464052; sminL=40.0; seq_a_single=45; score_norm=3.99949625741; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddcdddd +@HELIUM_000100422_612GNAAXX:7:70:11798:2668#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=15a_F730814; experiment=wolf_diet; forward_tag=gaagtag; reverse_tag=gaagtag; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.8059701493; seq_a_deletion=0; seq_b_single=46; score=239.988667111; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.2727272727; sminL=40.0; seq_a_single=46; score_norm=3.8707849534; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccttt ++ +ddddddddddddddddddd~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dcdddddcddddddddbdd +@HELIUM_000100422_612GNAAXX:7:4:18261:9148#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=61; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.8796992481; seq_a_deletion=0; seq_b_single=45; score=235.937993338; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.1; avg_quality=77.3333333333; sminL=40.0; seq_a_single=45; score_norm=3.7450475133; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +eddddddddddddddddd~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~`~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:29:18416:16620#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=77.4179104478; seq_a_deletion=0; seq_b_single=46; score=231.873033258; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.0649350649; sminL=40.0; seq_a_single=46; score_norm=3.7398876332; seq_b_insertion=0; +ttagccctaaacacaagtaattattataacaaaattattcgccagagttctaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctg ++ +dad\bbbbddcdbdcdd`d~~~~~~~~~~~~~~~~~~~~~~~~~~~~zF||u~s~`~~~~~~~~~~~~~~~~~~~~~~~~~ddddddb^bbb`[Hb`dd\ +@HELIUM_000100422_612GNAAXX:7:32:18262:6966#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=0; tail_quality=66.4; seq_b_deletion=0; mid_quality=78.7537313433; seq_a_deletion=0; seq_b_single=46; score=239.975704028; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.1; avg_quality=77.1948051948; sminL=40.0; seq_a_single=46; score_norm=3.87057587142; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddddddcddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~`~~~~~~~~~~~~ddddddddddbdddddddd +@HELIUM_000100422_612GNAAXX:7:110:17877:11976#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=77.8805970149; seq_a_deletion=0; seq_b_single=46; score=223.833250149; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.4675324675; sminL=40.0; seq_a_single=46; score_norm=3.61021371209; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddcddddddddddd^d\b~`~~~~~~~~~~~~~~~~~~~~x~~~~~~~~~~w{xqv~~~~~~~~~~~~~~~~~~~b~~~bdddddddddddddadddd_ +@HELIUM_000100422_612GNAAXX:7:97:9416:9155#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.5895522388; seq_a_deletion=0; seq_b_single=46; score=231.872797448; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.0844155844; sminL=40.0; seq_a_single=46; score_norm=3.7398838298; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddedddddddddddddde~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~{~~~~~~b~~~~~~~~~~~~~~~bddaddddddddddeddddd +@HELIUM_000100422_612GNAAXX:7:72:8905:10715#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.2932330827; seq_a_deletion=0; seq_b_single=45; score=251.929473647; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.6862745098; sminL=40.0; seq_a_single=45; score_norm=3.99888053408; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddcdddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddcddd +@HELIUM_000100422_612GNAAXX:7:48:5296:16375#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.1278195489; seq_a_deletion=0; seq_b_single=45; score=251.994265764; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.5424836601; sminL=40.0; seq_a_single=45; score_norm=3.99990898038; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +cddbdddddaddddbddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddd`dbdaadddd +@HELIUM_000100422_612GNAAXX:7:53:6550:1163#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.8872180451; seq_a_deletion=0; seq_b_single=45; score=235.996766828; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.3333333333; sminL=40.0; seq_a_single=45; score_norm=3.74598042584; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgtttgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +ddaddddddddddddddd~b~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:120:1900:13295#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.1; seq_b_deletion=0; mid_quality=77.5149253731; seq_a_deletion=1; seq_b_single=46; score=219.874676069; seq_a_insertion=1; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.1558441558; sminL=40.0; seq_a_single=46; score_norm=3.54636574304; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactacgcgcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddcdddddcdddcddddcd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ppthFfpo~~~~~~~~~~~~~~~~~~~b~~~~ddddddcdddddcdddddd +@HELIUM_000100422_612GNAAXX:7:96:19317:7551#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=58; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=3; tail_quality=66.9; seq_b_deletion=0; mid_quality=76.3955223881; seq_a_deletion=0; seq_b_single=46; score=215.85731879; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=65.9; avg_quality=75.0974025974; sminL=40.0; seq_a_single=46; score_norm=3.48156965791; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddbb`dddcdddddddd_d~~~~~~~~~~~~~~~Z~~~~~~~~zzt{qx~~yhpppns_|~~~za~~~~|~~~~~~a|~q~cccccccccccbcXcbccc +@HELIUM_000100422_612GNAAXX:7:93:18556:10479#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.3731343284; seq_a_deletion=0; seq_b_single=46; score=223.913795643; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.9; avg_quality=76.8896103896; sminL=40.0; seq_a_single=46; score_norm=3.61151283295; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddddddddddddddddd~b~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~dddddddddddddbddddd +@HELIUM_000100422_612GNAAXX:7:44:19605:15509#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=3; tail_quality=67.0; seq_b_deletion=0; mid_quality=77.7313432836; seq_a_deletion=0; seq_b_single=46; score=223.916231984; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.3376623377; sminL=40.0; seq_a_single=46; score_norm=3.61155212878; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dadcddddddddddd_d_a~~~~~~~~~~~~~~y}z~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~_~~~p~~`k~`~~r~ddcddcadcdcddcddddd +@HELIUM_000100422_612GNAAXX:7:40:3610:8896#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=15a_F730814; experiment=wolf_diet; forward_tag=gaagtag; reverse_tag=gaagtag; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.828358209; seq_a_deletion=0; seq_b_single=46; score=239.967241748; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.2922077922; sminL=40.0; seq_a_single=46; score_norm=3.87043938304; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~~~dddddddddddddeddddd +@HELIUM_000100422_612GNAAXX:7:41:19763:3556#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=65.7; seq_b_deletion=0; mid_quality=78.5671641791; seq_a_deletion=0; seq_b_single=46; score=247.825851895; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.9805194805; sminL=40.0; seq_a_single=46; score_norm=3.99719115959; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddcddddddddddddcad~~~~~~~~~~~~~~~~~~~~~~~~~~~~{m{zj~~~~~~~~v~~~~~~~~~~~~~~~~~~~{dddddddddddddcddddd +@HELIUM_000100422_612GNAAXX:7:111:7660:7465#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=58; sminR=40.0; seq_b_mismatch=3; seq_a_mismatch=2; tail_quality=66.1; seq_b_deletion=0; mid_quality=76.8646616541; seq_a_deletion=0; seq_b_single=45; score=211.768626837; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=66.5; avg_quality=75.4836601307; sminL=40.0; seq_a_single=45; score_norm=3.36140677518; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +ddcdddddd`ddddddcd~~~~~~~~_~~~~~~~~~~~~~Ky~~~~~~c~Y}~~s~~`~x~}~}~{~Z|h~~~~~~~~~~~dYYdddaddaaddad^dd +@HELIUM_000100422_612GNAAXX:7:76:10822:18722#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=58; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=5; tail_quality=66.8; seq_b_deletion=0; mid_quality=77.3834586466; seq_a_deletion=0; seq_b_single=45; score=211.83390837; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=66.5; avg_quality=75.9803921569; sminL=40.0; seq_a_single=45; score_norm=3.36244299; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacacgaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +acbdcddddTbdcddddd~`~~~z~~~~~~S~~~~~~b~~`~~~t~t~~}~~~~~~~~~~~T~~~|~~~~~~~~~~~~x~~ddddadddd_ddbdcbdd +@HELIUM_000100422_612GNAAXX:7:76:8491:8900#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=57; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=4; tail_quality=65.3; seq_b_deletion=0; mid_quality=76.552238806; seq_a_deletion=0; seq_b_single=46; score=207.828655885; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.9; avg_quality=75.1948051948; sminL=40.0; seq_a_single=46; score_norm=3.35207509492; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtgctacctgcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +bcccccccbcccc_ccb[b~^~~~~~~~~~~~~~uw~~~~}~~~y~~}N~qx~}F}z~~~~~~~~~~~~~o~~~~~b~~~bdddddddddddcdadddda +@HELIUM_000100422_612GNAAXX:7:47:7619:9866#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.1; seq_b_deletion=0; mid_quality=78.8358208955; seq_a_deletion=0; seq_b_single=46; score=239.867362445; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.1; avg_quality=77.3116883117; sminL=40.0; seq_a_single=46; score_norm=3.86882842653; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddddddddddddddded~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~addddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:99:14742:5591#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.5895522388; seq_a_deletion=0; seq_b_single=46; score=231.982488051; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.0844155844; sminL=40.0; seq_a_single=46; score_norm=3.74165303308; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddddddddddddddddcd~b~~~~~~~~~~~~~a~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddddcdd +@HELIUM_000100422_612GNAAXX:7:95:3274:5435#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=61; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=1; tail_quality=66.8; seq_b_deletion=0; mid_quality=78.8421052632; seq_a_deletion=0; seq_b_single=45; score=235.944669323; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.2810457516; sminL=40.0; seq_a_single=45; score_norm=3.74515348131; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddcdddddddddcdddd~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~{~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~ddddddddddcddddddd +@HELIUM_000100422_612GNAAXX:7:74:8396:18135#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.8045112782; seq_a_deletion=0; seq_b_single=45; score=235.858013544; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.2614379085; sminL=40.0; seq_a_single=45; score_norm=3.74377799276; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +ddddddddddddddcddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~W~dddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:9:10358:4089#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=61; seq_ab_match=54; sminR=40.0; seq_b_mismatch=6; seq_a_mismatch=1; tail_quality=66.9; seq_b_deletion=1; mid_quality=66.3880597015; seq_a_deletion=0; seq_b_single=47; score=181.620394786; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.9; avg_quality=66.4545454545; sminL=40.0; seq_a_single=46; score_norm=2.97738352108; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactacaggcaatagctcaaaactcaaagaacttggcggtgctttataccctt ++ +d^dadbaddddddcddcd^`zy~XwzzzZ~~~ccccccccccccccccBccccBccccccccccBcccccccccccBcccacc`ddc][\[]]`addddd +@HELIUM_000100422_612GNAAXX:7:78:10338:12302#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.6119402985; seq_a_deletion=0; seq_b_single=46; score=231.881878067; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.9; avg_quality=77.0974025974; sminL=40.0; seq_a_single=46; score_norm=3.74003029141; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddddddddddddddddd~a~~~~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddedddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:95:17941:6113#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=57; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=6; tail_quality=66.1; seq_b_deletion=0; mid_quality=77.9172932331; seq_a_deletion=0; seq_b_single=45; score=203.869927289; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.1; avg_quality=76.4379084967; sminL=40.0; seq_a_single=45; score_norm=3.23603059189; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +ddd`dd`cdddc^edeca~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~~~~~~~~bb~~~b~~b~~~b~dddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:59:11971:12063#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.9850746269; seq_a_deletion=0; seq_b_single=46; score=247.995051728; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.4285714286; sminL=40.0; seq_a_single=46; score_norm=3.99992018915; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgtcagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +acddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~|~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddcddddd +@HELIUM_000100422_612GNAAXX:7:47:3878:6141#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.0223880597; seq_a_deletion=0; seq_b_single=46; score=247.947034203; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.461038961; sminL=40.0; seq_a_single=46; score_norm=3.99914571295; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:21:5487:19582#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=58; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=3; tail_quality=67.1; seq_b_deletion=0; mid_quality=77.6641791045; seq_a_deletion=0; seq_b_single=46; score=215.831528178; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.5; avg_quality=76.2532467532; sminL=40.0; seq_a_single=46; score_norm=3.4811536803; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dbddcdddcddddddddd_~v}~~}{~~~~b~}ZZ}}}t~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~d`dbdddbdcdcdacdddd +@HELIUM_000100422_612GNAAXX:7:38:13721:2004#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.5298507463; seq_a_deletion=0; seq_b_single=46; score=239.856433937; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.3; avg_quality=76.987012987; sminL=40.0; seq_a_single=46; score_norm=3.86865216027; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +a]ddddddddddddddd`d~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~}}~~~~~~~~~~~~~~~b~~~~~~~~~~~~{~dddddddddbadd^dbddb +@HELIUM_000100422_612GNAAXX:7:111:19168:18517#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=15a_F730814; experiment=wolf_diet; forward_tag=gaagtag; reverse_tag=gaagtag; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=60; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=77.8955223881; seq_a_deletion=0; seq_b_single=46; score=223.825469474; seq_a_insertion=1; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.4805194805; sminL=40.0; seq_a_single=45; score_norm=3.55278522975; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattatccgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddbddddddbcdddd^dd~~~{~_~~~~~~~~~y{}{~U~{|~~~~~n~~|~~~~}x~~~~~~~~~~~~~~~~~^~~~~~~ccdaddbdddbddb_dddd +@HELIUM_000100422_612GNAAXX:7:89:16162:8889#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.1278195489; seq_a_deletion=0; seq_b_single=45; score=243.971622168; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.2; avg_quality=77.5555555556; sminL=40.0; seq_a_single=45; score_norm=3.87256543123; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddddddddddddddddd~~c~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddedddddddeddddd +@HELIUM_000100422_612GNAAXX:7:74:4888:6953#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=61; sminR=40.0; seq_b_mismatch=2; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.8571428571; seq_a_deletion=0; seq_b_single=45; score=235.986171514; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.3071895425; sminL=40.0; seq_a_single=45; score_norm=3.74581224625; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~]~~~~~~~~~~~~~~~~~~~dddddddddedddddddd +@HELIUM_000100422_612GNAAXX:7:41:16707:19819#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.3656716418; seq_a_deletion=0; seq_b_single=46; score=231.94731954; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.1; avg_quality=76.8961038961; sminL=40.0; seq_a_single=46; score_norm=3.74108579903; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddddddddddddddddbb~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~bddad`a^dddbddaddddd +@HELIUM_000100422_612GNAAXX:7:7:7916:16505#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=4; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=58; seq_ab_match=58; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=93.0; seq_b_deletion=50; mid_quality=93.0; seq_a_deletion=50; seq_b_single=0; score=231.999536374; seq_a_insertion=0; mode=alignment; seq_length_ori=58; head_quality=93.0; avg_quality=93.0; sminL=40.0; seq_a_single=0; score_norm=3.99999200644; seq_b_insertion=0; +tttt ++ +~~~~ +@HELIUM_000100422_612GNAAXX:7:41:9316:2256#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.6343283582; seq_a_deletion=0; seq_b_single=46; score=239.993848494; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.1233766234; sminL=40.0; seq_a_single=46; score_norm=3.87086852409; seq_b_insertion=0; +ttagccctaaacacaagaaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddddddddddddddddNd~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddcadeddddd +@HELIUM_000100422_612GNAAXX:7:67:7582:3642#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=62; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.0902255639; seq_a_deletion=0; seq_b_single=45; score=243.962430842; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.5098039216; sminL=40.0; seq_a_single=45; score_norm=3.87241953718; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~`~~~~~~~~dddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:42:8435:4155#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.2857142857; seq_a_deletion=0; seq_b_single=45; score=251.987540492; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.1; avg_quality=77.6862745098; sminL=40.0; seq_a_single=45; score_norm=3.99980223003; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +ddddddddddd`dddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:33:9900:9541#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.8955223881; seq_a_deletion=0; seq_b_single=46; score=247.949713348; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.3506493506; sminL=40.0; seq_a_single=46; score_norm=3.99918892497; seq_b_insertion=0; +ctagccctaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddddd\`cccccdddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddedddddddddecddcdd +@HELIUM_000100422_612GNAAXX:7:48:7139:3789#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.3157894737; seq_a_deletion=0; seq_b_single=45; score=251.902451987; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.7058823529; sminL=40.0; seq_a_single=45; score_norm=3.99845161885; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:46:13269:4134#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.3157894737; seq_a_deletion=0; seq_b_single=45; score=251.997344433; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.7058823529; sminL=40.0; seq_a_single=45; score_norm=3.99995784814; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddcddddddddddd +@HELIUM_000100422_612GNAAXX:7:46:9246:19171#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=52; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=10; tail_quality=65.2; seq_b_deletion=0; mid_quality=70.7611940299; seq_a_deletion=0; seq_b_single=46; score=167.802718427; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.7; avg_quality=70.1363636364; sminL=40.0; seq_a_single=46; score_norm=2.70649545851; seq_b_insertion=0; +ttagccctaaacacaagtaattattataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +`dddddddddd^bddadcd~b~~~~~b~~~~~~~_`~~~~~b~~~~~S~~~~~~~~~~b~~n~`~~k~zoY|~~~~jb~~~DDDDDDDDDDDDDDDDDD` +@HELIUM_000100422_612GNAAXX:7:37:12979:6595#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=4; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=58; seq_ab_match=58; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=93.0; seq_b_deletion=50; mid_quality=93.0; seq_a_deletion=50; seq_b_single=0; score=231.998796685; seq_a_insertion=0; mode=alignment; seq_length_ori=58; head_quality=93.0; avg_quality=93.0; sminL=40.0; seq_a_single=0; score_norm=3.99997925319; seq_b_insertion=0; +tttt ++ +~~~~ +@HELIUM_000100422_612GNAAXX:7:95:1688:9598#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=54; sminR=40.0; seq_b_mismatch=3; seq_a_mismatch=6; tail_quality=66.8; seq_b_deletion=0; mid_quality=74.2932330827; seq_a_deletion=0; seq_b_single=45; score=179.847313607; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=65.7; avg_quality=73.2418300654; sminL=40.0; seq_a_single=45; score_norm=2.8547192636; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtgatactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddc`ddddcda`bcabb~~[~~~~~~~~~~~~~__~~|z~}}}~~SPF|mxxrwwtIwjxwvv{p~rwxzx`~~Wp~~~~d[ddd`[`^^dcdddd_d +@HELIUM_000100422_612GNAAXX:7:55:16528:11418#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=58; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=4; tail_quality=57.0; seq_b_deletion=0; mid_quality=76.0746268657; seq_a_deletion=0; seq_b_single=46; score=215.988419267; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.5; avg_quality=74.2142857143; sminL=40.0; seq_a_single=46; score_norm=3.48368418172; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcactagcttacaactcaaaggacttggcggtgctttataccctt ++ +ddcdddddddddddcd`da~~~~~~~~~~~~x~~~~~\~~~~~~~~~v~~~~~~~~~~\~~~~~~~[~~~s~~~~~~~~]~cY__]\QPPPP`]`[`c^^ +@HELIUM_000100422_612GNAAXX:7:32:18486:7018#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.3233082707; seq_a_deletion=0; seq_b_single=45; score=251.909476689; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.1; avg_quality=77.7189542484; sminL=40.0; seq_a_single=45; score_norm=3.99856312205; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +ddddddddddddddedcd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddedddddddd +@HELIUM_000100422_612GNAAXX:7:85:2105:21152#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=57; sminR=40.0; seq_b_mismatch=4; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=73.8947368421; seq_a_deletion=0; seq_b_single=45; score=203.706262238; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=72.9934640523; sminL=40.0; seq_a_single=45; score_norm=3.23343273394; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +daddddddddddaddd`d~b~~~~b~s`~b~~~~~~~~~j~x~~~cccccccccccccccccc~~~~\}~}~~~~~{~~b~dbddddedddeddcdddd +@HELIUM_000100422_612GNAAXX:7:83:6795:14061#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.9328358209; seq_a_deletion=0; seq_b_single=46; score=247.992382324; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.3831168831; sminL=40.0; seq_a_single=46; score_norm=3.99987713426; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddddddddddddddd_d~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddcddddddddddbdddbd +@HELIUM_000100422_612GNAAXX:7:73:16525:10764#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.3059701493; seq_a_deletion=0; seq_b_single=46; score=231.90336822; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.9; avg_quality=76.8311688312; sminL=40.0; seq_a_single=46; score_norm=3.74037690678; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +c_dddddddcddcdddd^b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~|~~~~~b~b~~~~~~~~~~~~~~dddddd_bddddb[dddda +@HELIUM_000100422_612GNAAXX:7:19:6313:16567#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=3; tail_quality=67.0; seq_b_deletion=0; mid_quality=77.4135338346; seq_a_deletion=0; seq_b_single=45; score=227.826165596; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=66.6; avg_quality=76.0261437908; sminL=40.0; seq_a_single=45; score_norm=3.61628834279; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaagggcttggcggtgctttacatccct ++ +ddddcddddddacddddd~~~~~~~~~~~~~~~~~~~~~}}w|~r~xvwrm{z~~~|a~~~~~~~}u~~}~~~~~_O~~~~ddcddb_b__`baYdcdd +@HELIUM_000100422_612GNAAXX:7:114:8865:19510#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.8059701493; seq_a_deletion=0; seq_b_single=46; score=239.913762365; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.1; avg_quality=77.2792207792; sminL=40.0; seq_a_single=46; score_norm=3.86957681233; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddddddddddddddddd~a~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddcddddddd +@HELIUM_000100422_612GNAAXX:7:111:8478:1127#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=57; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=5; tail_quality=67.0; seq_b_deletion=0; mid_quality=76.3909774436; seq_a_deletion=0; seq_b_single=45; score=203.794981474; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=66.4; avg_quality=75.1241830065; sminL=40.0; seq_a_single=45; score_norm=3.23484097578; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctggaacgcaaaggacttggcggtgctttacatccct ++ +dddaddddddddddMbdd~b~~~|~~~~~~{~~~~~~~zy|~~~~{~pv}y~~|~~~~~~~}~|~HxxwIup`~~Z~~v]~adddddacdddbad^dcd +@HELIUM_000100422_612GNAAXX:7:81:15726:2345#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=56; sminR=40.0; seq_b_mismatch=7; seq_a_mismatch=0; tail_quality=62.3; seq_b_deletion=0; mid_quality=63.1954887218; seq_a_deletion=0; seq_b_single=45; score=195.641193686; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=62.5; avg_quality=63.091503268; sminL=40.0; seq_a_single=45; score_norm=3.10541577279; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaagcactcggcggtgctttacatccct ++ +WJXc_bcca_ab__cc_b~~s~~{~~sww~pq~~~~cBccBccccccBcBccccccccccBccccccccccccccBcccBcDDDDDDDDDD`cbQc___ +@HELIUM_000100422_612GNAAXX:7:13:14386:20412#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=60; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.4552238806; seq_a_deletion=0; seq_b_single=45; score=223.918527801; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.9675324675; sminL=40.0; seq_a_single=46; score_norm=3.55426234604; seq_b_insertion=1; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +decddddddddddddddd~~a~~~~xa~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~t~~~~~~~~~~~~~~~~~~b~~~~ddddddddddddddddddc +@HELIUM_000100422_612GNAAXX:7:83:18882:9967#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=60; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.6466165414; seq_a_deletion=0; seq_b_single=45; score=227.922890965; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.1241830065; sminL=40.0; seq_a_single=45; score_norm=3.6178236661; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddddddddddddddddd~b~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~~ddddddddddddddddda +@HELIUM_000100422_612GNAAXX:7:13:17353:13234#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=2; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=77.6417910448; seq_a_deletion=0; seq_b_single=46; score=223.801902424; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.1; avg_quality=76.2662337662; sminL=40.0; seq_a_single=46; score_norm=3.60970810361; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccttt ++ +ddddddddddddeddWd`b~~}~~~~~x~~~~~oVp]~~~~~~~~~~~~~|~~~~~~~~~~~~~~~~~~~~~~~~~~~~~bdaddbddbdddbcUcbddd +@HELIUM_000100422_612GNAAXX:7:53:10153:11845#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=15a_F730814; experiment=wolf_diet; forward_tag=gaagtag; reverse_tag=gaagtag; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.8059701493; seq_a_deletion=0; seq_b_single=46; score=239.985596162; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.2727272727; sminL=40.0; seq_a_single=46; score_norm=3.87073542196; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddddddddddddddddd~~~~~~~~~~~~~~~a~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddeddddd +@HELIUM_000100422_612GNAAXX:7:33:9296:13526#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.5597014925; seq_a_deletion=0; seq_b_single=46; score=239.852481933; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.7; avg_quality=77.038961039; sminL=40.0; seq_a_single=46; score_norm=3.86858841828; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dcdddddeedddddddd^d~~~~~~~~~~~~~~~~~~~~~~~~~~~~~|~|~~~~~v~~~~~~~~~~~~~~~~~~~~~~~^dddddddbdddddZdddcd +@HELIUM_000100422_612GNAAXX:7:113:2918:18736#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=58; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=4; tail_quality=67.0; seq_b_deletion=0; mid_quality=77.828358209; seq_a_deletion=0; seq_b_single=46; score=215.87931956; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.4220779221; sminL=40.0; seq_a_single=46; score_norm=3.48192450903; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dcdcdddddddddddd_`d~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~v~~~v~~y`~~y~~~~~~~~~~~b~~b~~~bdcddddcddddda_ddddd +@HELIUM_000100422_612GNAAXX:7:63:4725:12502#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.0671641791; seq_a_deletion=0; seq_b_single=46; score=239.998975242; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.5; avg_quality=76.5974025974; sminL=40.0; seq_a_single=46; score_norm=3.87095121358; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dcdcddeddddddddWd]d~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~~dddddd``ddd]\R]\ddT +@HELIUM_000100422_612GNAAXX:7:7:11110:17924#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=15a_F730814; experiment=wolf_diet; forward_tag=gaagtag; reverse_tag=gaagtag; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=58; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=3; tail_quality=67.0; seq_b_deletion=0; mid_quality=75.723880597; seq_a_deletion=0; seq_b_single=46; score=215.812113201; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.4; avg_quality=74.5519480519; sminL=40.0; seq_a_single=46; score_norm=3.4808405355; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtcataccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dcddddddddcdddddd`d~b~~~~~~~~~~~~~~~~~rm{kripoooEJpoolppp~~~~~~~~~~~~~v~~~~~`v~~~dddcdddddddbddddadd +@HELIUM_000100422_612GNAAXX:7:102:6364:4627#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.2932330827; seq_a_deletion=0; seq_b_single=45; score=251.985885517; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.6862745098; sminL=40.0; seq_a_single=45; score_norm=3.99977596059; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddcddddddddddcdddd +@HELIUM_000100422_612GNAAXX:7:96:8737:4973#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=4; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=58; seq_ab_match=57; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=93.0; seq_b_deletion=50; mid_quality=92.2631578947; seq_a_deletion=50; seq_b_single=0; score=223.980140585; seq_a_insertion=0; mode=alignment; seq_length_ori=58; head_quality=93.0; avg_quality=92.5172413793; sminL=40.0; seq_a_single=0; score_norm=3.86172656181; seq_b_insertion=0; +tttt ++ +~b~~ +@HELIUM_000100422_612GNAAXX:7:89:9710:20652#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=62; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.8421052632; seq_a_deletion=0; seq_b_single=45; score=243.910293117; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.2941176471; sminL=40.0; seq_a_single=45; score_norm=3.87159195424; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttatatccct ++ +dad]ccccddcdddcddd~~}~~~~~~~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~}ddaddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:71:17473:7401#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=77.2089552239; seq_a_deletion=0; seq_b_single=46; score=231.883938289; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=65.5; avg_quality=75.7857142857; sminL=40.0; seq_a_single=46; score_norm=3.74006352079; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggtaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddaddddccdbddbddd~~~~~~~~b~~~~~~~~~~~~~~~~~~~~~~~~wywop~~~~gx~~~~~w~~s~~~~R~~~~c^cc_ccccc^caabaccc +@HELIUM_000100422_612GNAAXX:7:28:9406:18226#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.4925373134; seq_a_deletion=0; seq_b_single=46; score=231.816671723; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.0; sminL=40.0; seq_a_single=46; score_norm=3.73897857618; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddddddddddcdcdecd~~~~~~~~~~~~~~~~|~~~~~~~~~~~~~~{~~~~~~~~~~x~~~~~~~~~~~~~~b~~~_ddeddddddddddcddddd +@HELIUM_000100422_612GNAAXX:7:15:16118:15111#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=15a_F730814; experiment=wolf_diet; forward_tag=gaagtag; reverse_tag=gaagtag; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.4104477612; seq_a_deletion=0; seq_b_single=46; score=231.917355899; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.9285714286; sminL=40.0; seq_a_single=46; score_norm=3.7406025145; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddddddddddddd`d]d~~~c~~~~x~~~~~~a~~~w~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddd`dddeddddd +@HELIUM_000100422_612GNAAXX:7:58:18618:20959#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=2; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.2537313433; seq_a_deletion=0; seq_b_single=46; score=223.882241304; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.1; avg_quality=76.7987012987; sminL=40.0; seq_a_single=46; score_norm=3.611003892; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddcddddddddddddddddb~~~~~b~~~~~~~~~x~~z~~~~~|~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b}~~~ddddddcdddddd_ddddd +@HELIUM_000100422_612GNAAXX:7:46:11654:6037#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.4402985075; seq_a_deletion=0; seq_b_single=46; score=239.828425953; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.9545454545; sminL=40.0; seq_a_single=46; score_norm=3.8682004186; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddedddcdddddddddcd~~~~~~~~~~~~~~~~~~~~~~~~~~~}{z~~v~~~{~~~~~~~}~~~~~~~~~~~~~~~~bcccddcb^bbbcbdddddd +@HELIUM_000100422_612GNAAXX:7:2:6320:8068#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.3082706767; seq_a_deletion=0; seq_b_single=45; score=251.910304163; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.1; avg_quality=77.7058823529; sminL=40.0; seq_a_single=45; score_norm=3.99857625655; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddeddddbddddddd +@HELIUM_000100422_612GNAAXX:7:46:1592:11866#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=66.5; seq_b_deletion=0; mid_quality=78.6791044776; seq_a_deletion=0; seq_b_single=46; score=247.993604064; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.8; avg_quality=77.1168831169; sminL=40.0; seq_a_single=46; score_norm=3.99989683974; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dd_dddbcd\dddddd]dd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddcddcddd`dddcddcdd +@HELIUM_000100422_612GNAAXX:7:119:2660:19129#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=61; sminR=40.0; seq_b_mismatch=2; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.6541353383; seq_a_deletion=0; seq_b_single=45; score=235.859804076; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.1307189542; sminL=40.0; seq_a_single=45; score_norm=3.74380641391; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dcddcddddddddcdddd~~~~~~~~~~~~~~~~z}~~~~~~~~~~~~~~~~~~~~~~~~~~~~k~~~~~~~~~~b~^~~~dddbdddddddddddddd +@HELIUM_000100422_612GNAAXX:7:115:3625:10051#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=5; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=59; seq_ab_match=59; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=93.0; seq_b_deletion=49; mid_quality=93.0; seq_a_deletion=49; seq_b_single=0; score=235.999642017; seq_a_insertion=0; mode=alignment; seq_length_ori=59; head_quality=93.0; avg_quality=93.0; sminL=40.0; seq_a_single=0; score_norm=3.99999393249; seq_b_insertion=0; +caata ++ +~~~~~ +@HELIUM_000100422_612GNAAXX:7:100:17253:5513#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=65.1; seq_b_deletion=0; mid_quality=76.6417910448; seq_a_deletion=0; seq_b_single=46; score=231.761122002; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.7; avg_quality=75.2467532468; sminL=40.0; seq_a_single=46; score_norm=3.73808261294; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddbdddddcddddddddd~~~~|{|~~~~b~~~~~~~~~~s~~~~~x~~~~~~~rs~zqwxy~z~~|}~z~}|{^v~~~~c``Zc`cXWcc[WYYZccc +@HELIUM_000100422_612GNAAXX:7:82:6036:6921#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=2; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.4104477612; seq_a_deletion=0; seq_b_single=46; score=231.901981419; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.9285714286; sminL=40.0; seq_a_single=46; score_norm=3.74035453901; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddcbddddeddddddddcd~~~~~b~~~~~~~~~~~~~~~~~~~~~~~]~~~~~~~~}~~~~~~~~~~~~~~~~~~~|~{~dddddccdcddddabddd` +@HELIUM_000100422_612GNAAXX:7:120:4726:20808#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.6940298507; seq_a_deletion=0; seq_b_single=46; score=247.810697755; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.9; avg_quality=77.1688311688; sminL=40.0; seq_a_single=46; score_norm=3.99694673798; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dbedddddcdddddddddb~~~~~~~~~~~~~~ty}y~~~~~~~~~~~~{~~~~~~~~~~~~~~~~~~~~~~~~~~~~~r~ddddcdddddcdd`ddddd +@HELIUM_000100422_612GNAAXX:7:99:8183:13912#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=1; tail_quality=67.1; seq_b_deletion=0; mid_quality=78.3358208955; seq_a_deletion=0; seq_b_single=46; score=231.903850642; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.8701298701; sminL=40.0; seq_a_single=46; score_norm=3.74038468777; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctc ++ +ddddddddddcddddddeb~Z~~~~~~~~~~~~~~~~~}~l~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~dadcdedddcdddddddd` +@HELIUM_000100422_612GNAAXX:7:10:4085:7492#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=62; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.1052631579; seq_a_deletion=0; seq_b_single=45; score=243.95581367; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.522875817; sminL=40.0; seq_a_single=45; score_norm=3.87231450269; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~dddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:35:12723:18557#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.1804511278; seq_a_deletion=0; seq_b_single=45; score=251.945522222; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.5882352941; sminL=40.0; seq_a_single=45; score_norm=3.99913527337; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +adddcddddddddddddd~~}~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~cdddcdddddddbddddd +@HELIUM_000100422_612GNAAXX:7:97:10495:17046#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=0; tail_quality=66.9; seq_b_deletion=0; mid_quality=78.7686567164; seq_a_deletion=0; seq_b_single=46; score=239.938979134; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.1; avg_quality=77.2402597403; sminL=40.0; seq_a_single=46; score_norm=3.86998353443; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~a~~~~~~~~~~~~dddddddddddddddcddd +@HELIUM_000100422_612GNAAXX:7:73:17068:16620#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=4; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=58; seq_ab_match=57; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=0; tail_quality=92.8; seq_b_deletion=50; mid_quality=92.2631578947; seq_a_deletion=50; seq_b_single=0; score=223.962007947; seq_a_insertion=0; mode=alignment; seq_length_ori=58; head_quality=93.0; avg_quality=92.4827586207; sminL=40.0; seq_a_single=0; score_norm=3.86141393012; seq_b_insertion=0; +tttt ++ +~~~~ +@HELIUM_000100422_612GNAAXX:7:23:13304:5167#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.0149253731; seq_a_deletion=0; seq_b_single=46; score=247.997553566; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.4545454545; sminL=40.0; seq_a_single=46; score_norm=3.99996054139; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddcddddd +@HELIUM_000100422_612GNAAXX:7:54:2542:19180#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.7313432836; seq_a_deletion=0; seq_b_single=46; score=239.868258533; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.2077922078; sminL=40.0; seq_a_single=46; score_norm=3.86884287957; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddddddddddddddbdad~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~|~~~~~~~~~~~~~~~~~b~~~~dddddccdcddddbddddd +@HELIUM_000100422_612GNAAXX:7:109:17917:15159#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.8571428571; seq_a_deletion=0; seq_b_single=45; score=235.857230348; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.1; avg_quality=77.3137254902; sminL=40.0; seq_a_single=45; score_norm=3.74376556108; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddcdddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~}~~~~~~~~~~~~~~~~~~~~~a~~b~~~~~cddddddddddddcdddd +@HELIUM_000100422_612GNAAXX:7:59:18371:8149#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.0149253731; seq_a_deletion=0; seq_b_single=46; score=247.998742319; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.4545454545; sminL=40.0; seq_a_single=46; score_norm=3.99997971482; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dcddddddddddddddddc~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddeddddd +@HELIUM_000100422_612GNAAXX:7:86:11013:15234#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.8208955224; seq_a_deletion=0; seq_b_single=46; score=239.990009212; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.2857142857; sminL=40.0; seq_a_single=46; score_norm=3.8708066002; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddddddddddddddddd~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:77:18518:10758#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=2; tail_quality=62.5; seq_b_deletion=0; mid_quality=72.2388059701; seq_a_deletion=0; seq_b_single=46; score=223.70605163; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=63.9; avg_quality=71.0649350649; sminL=40.0; seq_a_single=46; score_norm=3.60816212307; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +caccaccccccccccc_cc~~~~~~~~~~~~wzz{~~a~~~~y~_~~~~~~~~~~m~sygwqv~ccBccccccccccccc~bb^^bc`U\\`\]XPZ\__ +@HELIUM_000100422_612GNAAXX:7:63:18851:7688#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=3; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.4104477612; seq_a_deletion=0; seq_b_single=46; score=223.854412144; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.9285714286; sminL=40.0; seq_a_single=46; score_norm=3.61055503459; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dcddddddddddedddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~a~b~~~~~~~~~b~~~~dddddddddddeddddddd +@HELIUM_000100422_612GNAAXX:7:22:1798:3790#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=3; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.3208955224; seq_a_deletion=0; seq_b_single=46; score=223.82253574; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.8506493506; sminL=40.0; seq_a_single=46; score_norm=3.61004089903; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtgctaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dadbddbdddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~~~~~b~~~bdddddddddddddaddddd +@HELIUM_000100422_612GNAAXX:7:115:14354:10118#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=59; sminR=40.0; seq_b_mismatch=2; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.045112782; seq_a_deletion=0; seq_b_single=45; score=219.850378027; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=76.6013071895; sminL=40.0; seq_a_single=45; score_norm=3.4896885401; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactcgcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +ddeddddddddeddddcd~b~~~~~~~~~~~~_~~~~~~~~~~~~~~~w|y~tJ}~~~~~~}~~~~~~~~~~~~~b~~~~~ddddddddcddccddddd +@HELIUM_000100422_612GNAAXX:7:99:14794:3606#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=61; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.7894736842; seq_a_deletion=0; seq_b_single=45; score=235.971679218; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=66.9; avg_quality=77.2418300654; sminL=40.0; seq_a_single=45; score_norm=3.74558220981; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddddddddddddddddd~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~dddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:74:8297:20108#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=15a_F730814; experiment=wolf_diet; forward_tag=gaagtag; reverse_tag=gaagtag; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.776119403; seq_a_deletion=0; seq_b_single=46; score=239.962155962; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.2467532468; sminL=40.0; seq_a_single=46; score_norm=3.87035735423; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddcdddddddddddddd`d~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~}~ddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:29:12308:10119#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.3233082707; seq_a_deletion=0; seq_b_single=45; score=251.866768717; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.7124183007; sminL=40.0; seq_a_single=45; score_norm=3.99788521773; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddddddeddddedcddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:25:11691:21134#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.1567164179; seq_a_deletion=0; seq_b_single=46; score=223.86269299; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.7077922078; sminL=40.0; seq_a_single=46; score_norm=3.61068859661; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddcdddddcddddddad]d~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~}~}~~~~~~~~~~~~~~~}~~b~~~_dddddddadddcdaddddd +@HELIUM_000100422_612GNAAXX:7:111:15484:19248#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=60; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=77.954887218; seq_a_deletion=0; seq_b_single=45; score=227.828264164; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=76.522875817; sminL=40.0; seq_a_single=45; score_norm=3.6163216534; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +ddddddddddddddaddd~~~~~~~~~~~~~~~`~~jzvxt{~z~~~{~}yx~}~~{`~~~~~~~~s~~~~~~~~b~~~~~dddddddddd`ddcdddd +@HELIUM_000100422_612GNAAXX:7:103:3594:15996#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.3805970149; seq_a_deletion=0; seq_b_single=46; score=231.92194325; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.1; avg_quality=76.9090909091; sminL=40.0; seq_a_single=46; score_norm=3.74067650404; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacacaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddddddddddddddddddb~~~~~~~~~~~R~}~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddaddddd +@HELIUM_000100422_612GNAAXX:7:1:14576:14181#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=15a_F730814; experiment=wolf_diet; forward_tag=gaagtag; reverse_tag=gaagtag; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.5373134328; seq_a_deletion=0; seq_b_single=46; score=223.962708229; seq_a_insertion=1; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.038961039; sminL=40.0; seq_a_single=45; score_norm=3.55496362269; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddddddcddddddcda~~~~b~~~~~~~~~~~a~~~~~~`~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddedddddcddddd +@HELIUM_000100422_612GNAAXX:7:99:13467:17714#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=2; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.3432835821; seq_a_deletion=0; seq_b_single=46; score=223.932198733; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.9; avg_quality=76.8636363636; sminL=40.0; seq_a_single=46; score_norm=3.61180965699; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddcddddddddddddddcd~~~~~~~~b~~~}~~~~~~~~~~~~~~~~~~~~~~~^~~~~~~~~~~~~~~~~~~~~~~~~bddddddddddeddcddddd +@HELIUM_000100422_612GNAAXX:7:43:7980:7908#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.5970149254; seq_a_deletion=0; seq_b_single=46; score=239.984831014; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.9; avg_quality=77.0844155844; sminL=40.0; seq_a_single=46; score_norm=3.87072308087; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +daddcdddd_cddcdddda~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~`~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~d`ddddddddddccbdddd +@HELIUM_000100422_612GNAAXX:7:105:14135:6905#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.8134328358; seq_a_deletion=0; seq_b_single=46; score=239.985379811; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.2792207792; sminL=40.0; seq_a_single=46; score_norm=3.87073193244; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcaccagagtactagcggcaacagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddddddddddddddddd~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddeddddddddddddbddd +@HELIUM_000100422_612GNAAXX:7:89:16663:15081#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.0149253731; seq_a_deletion=0; seq_b_single=46; score=247.939062327; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.1; avg_quality=77.461038961; sminL=40.0; seq_a_single=46; score_norm=3.9990171343; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddecddddd +@HELIUM_000100422_612GNAAXX:7:86:15907:21191#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=58; sminR=40.0; seq_b_mismatch=2; seq_a_mismatch=3; tail_quality=67.0; seq_b_deletion=0; mid_quality=77.9172932331; seq_a_deletion=0; seq_b_single=45; score=211.91203832; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=66.9; avg_quality=76.4836601307; sminL=40.0; seq_a_single=45; score_norm=3.36368314793; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dd`deddddddddddddd~~~~~{~~~~~~~~~~~~~~~~~~~~~~~~X~~~[~~~~~~~~~~~~~~~~~~~`~{a~~~b~ddcd\ddadddddcdddb +@HELIUM_000100422_612GNAAXX:7:81:17261:2872#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.1; seq_b_deletion=0; mid_quality=79.3157894737; seq_a_deletion=0; seq_b_single=45; score=251.967130674; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=66.9; avg_quality=77.7058823529; sminL=40.0; seq_a_single=45; score_norm=3.99947826467; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddedddddddddedddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddadddddeddedddddd +@HELIUM_000100422_612GNAAXX:7:14:15397:17819#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.171641791; seq_a_deletion=0; seq_b_single=46; score=223.847163266; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.9; avg_quality=76.7142857143; sminL=40.0; seq_a_single=46; score_norm=3.61043811719; seq_b_insertion=0; +ttagccctaaacacaaataattacacaaacaaaattgttcaccagagtactagcggcaacagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddddddddddddddddc~`~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~a~s~~~v~~~~~~~~}~~a~~~~~~~dddddcbddddddcddddd +@HELIUM_000100422_612GNAAXX:7:76:18268:14980#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=66.9; seq_b_deletion=0; mid_quality=78.3208955224; seq_a_deletion=0; seq_b_single=46; score=239.985254914; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.8441558442; sminL=40.0; seq_a_single=46; score_norm=3.87072991797; seq_b_insertion=0; +ttacccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +d\ccdcddddddddddecd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~z~~~~~~~~~~~~~~~~~~~~b~~~~adbdbd^bcccddHddbdb +@HELIUM_000100422_612GNAAXX:7:16:15509:18069#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=58; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=4; tail_quality=66.8; seq_b_deletion=0; mid_quality=77.5; seq_a_deletion=0; seq_b_single=46; score=215.81634366; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.1233766234; sminL=40.0; seq_a_single=46; score_norm=3.48090876871; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +cdYdddddbdddbdcddda~~~~~~~~~~~~~~~~~~}a~r~~~~~~~~~~~~~~]zz~~~~}~~~~~~~~~~~~~b}}~_dddddddbdddddLaddbd +@HELIUM_000100422_612GNAAXX:7:25:7789:3090#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=3; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=72.4179104478; seq_a_deletion=0; seq_b_single=46; score=223.705772044; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.5; avg_quality=71.6818181818; sminL=40.0; seq_a_single=46; score_norm=3.60815761361; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactacaggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ead]dddddddddddddbd~~~~~~~~~~~~{~}~~~~cBBccccccccccccBccc~~z}~y~p~~~w|j||~~~uzr|tNba^\bT^cccZ\L\]`_` +@HELIUM_000100422_612GNAAXX:7:53:18419:8404#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.5447761194; seq_a_deletion=0; seq_b_single=46; score=231.841887233; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.0454545455; sminL=40.0; seq_a_single=46; score_norm=3.73938527796; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +decddddddddddddddddb~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~{~~~~b~~~~~~~~~~~~~~~{~~~~~~~~~ddddddedddddd_ddddd +@HELIUM_000100422_612GNAAXX:7:19:11039:3957#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.8432835821; seq_a_deletion=0; seq_b_single=46; score=247.811165598; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.7; avg_quality=77.2857142857; sminL=40.0; seq_a_single=46; score_norm=3.99695428384; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddcdddddddddddddad~~~~~~~~~~~~~~~~~~~~~~~~~~~}~~~~~~~~~~v~~~~~~~~~~~~~~~~~~~~~~~ddbdddbadddddbddddd +@HELIUM_000100422_612GNAAXX:7:42:19757:3800#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=57; sminR=40.0; seq_b_mismatch=3; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=1; mid_quality=77.7669172932; seq_a_deletion=1; seq_b_single=46; score=187.78301321; seq_a_insertion=1; mode=alignment; seq_length_ori=153; head_quality=67.1; avg_quality=76.3660130719; sminL=40.0; seq_a_single=45; score_norm=3.02875827758; seq_b_insertion=0; +ttagccctaaacacaagtaattaatatacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddedddeddddddcdcc~~~~~~~b~|~~~a~`~~~~~~~~~~~vz~~~~~~~~~~~x_~~~~~~~~~~~~~]~~~~~~dddddd`dddddadddcd +@HELIUM_000100422_612GNAAXX:7:116:14244:17926#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=55; sminR=40.0; seq_b_mismatch=3; seq_a_mismatch=5; tail_quality=67.0; seq_b_deletion=0; mid_quality=76.5338345865; seq_a_deletion=0; seq_b_single=45; score=187.882758912; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=75.2875816993; sminL=40.0; seq_a_single=45; score_norm=2.98226601448; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgcaagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +d`dbcccc`dddddeddd~~~~~~_~~~~b~~~~~~^xz{ZRzpooop~~s~~~~~~`~~~~~~~~~~~~~~~~~b~~~b~dddddedddddddddddd +@HELIUM_000100422_612GNAAXX:7:91:10071:8960#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.3233082707; seq_a_deletion=0; seq_b_single=45; score=251.979171474; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.7124183007; sminL=40.0; seq_a_single=45; score_norm=3.99966938848; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +ddddddddddddddeddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:61:17484:5085#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.9776119403; seq_a_deletion=0; seq_b_single=46; score=247.996979391; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.9; avg_quality=77.4155844156; sminL=40.0; seq_a_single=46; score_norm=3.9999512805; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddedddddddddddd`d~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddccddcddd +@HELIUM_000100422_612GNAAXX:7:18:2721:16829#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.3308270677; seq_a_deletion=0; seq_b_single=45; score=251.960802952; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.2; avg_quality=77.7320261438; sminL=40.0; seq_a_single=45; score_norm=3.99937782464; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddeeddddedddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dbdddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:2:7296:17275#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=2; seq_a_mismatch=1; tail_quality=66.9; seq_b_deletion=0; mid_quality=78.0298507463; seq_a_deletion=0; seq_b_single=46; score=223.923525795; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.3; avg_quality=76.5454545455; sminL=40.0; seq_a_single=46; score_norm=3.61166977089; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dbcacbcbd`ddddddd_b~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~vbdddddddddddcc]baddb +@HELIUM_000100422_612GNAAXX:7:58:8591:17028#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=2; tail_quality=66.0; seq_b_deletion=0; mid_quality=75.8582089552; seq_a_deletion=0; seq_b_single=46; score=223.788176649; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=65.3; avg_quality=74.5324675325; sminL=40.0; seq_a_single=46; score_norm=3.60948672015; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +_bcccccccbccacccccc~~~~~a~~y~~~~~wyn~~~~x~~zrzzkpppp~~~~~~{zuxx~~~~~z~xw~`~~_~~~~aaac]ca`cccccacc__[ +@HELIUM_000100422_612GNAAXX:7:13:3469:18630#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=58; sminR=40.0; seq_b_mismatch=2; seq_a_mismatch=3; tail_quality=66.0; seq_b_deletion=0; mid_quality=76.2631578947; seq_a_deletion=0; seq_b_single=45; score=211.840326012; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=66.3; avg_quality=74.9411764706; sminL=40.0; seq_a_single=45; score_norm=3.36254485733; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddIQQPPdadddcdadd~~~~~xyyLqV{w~~~~~~~~~~~~~~~~~~zszx{|yy{~~~~~~~~~~~~~~[~~b~~~b~ddddd_dddd^`a_cdd` +@HELIUM_000100422_612GNAAXX:7:72:12959:5736#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=56; sminR=40.0; seq_b_mismatch=7; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=63.5263157895; seq_a_deletion=0; seq_b_single=45; score=195.664863172; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=59.8; avg_quality=63.5098039216; sminL=40.0; seq_a_single=45; score_norm=3.10579147892; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacacccct ++ +^\bSbbbbddddd^dddd~~~~~~~~~~~~~~~b~~ib~~~~~~_~ccBBBcccccccccBccccccccccccccccccccDDDDDDDDDDDDDDDDDD +@HELIUM_000100422_612GNAAXX:7:115:6263:17700#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.6390977444; seq_a_deletion=0; seq_b_single=45; score=235.853550005; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.1176470588; sminL=40.0; seq_a_single=45; score_norm=3.74370714293; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +ddcdddddddddddeddd~~~~~~~~~~~~~~~~~~~~~~~~~~y|z{yxx~~{~~~~~~~~~~~~~~~~~~~~~b~~~b~ddddeddddddddddddd +@HELIUM_000100422_612GNAAXX:7:7:15538:8325#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=52; sminR=40.0; seq_b_mismatch=10; seq_a_mismatch=0; tail_quality=63.4; seq_b_deletion=1; mid_quality=60.1879699248; seq_a_deletion=0; seq_b_single=45; score=151.662770749; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=59.2; avg_quality=60.3333333333; sminL=40.0; seq_a_single=45; score_norm=2.40734556744; seq_b_insertion=1; +ttagccctaaacataaacattcaataaacaagaatgtcggccagagtactactagcaacagcatgaaactcaaagaactgggcggtgctttacatccct ++ +_S\abcacca_cacccc_~~y}~~~~qxYzgY^~~~accBccccccccccccccBBccccccBccccccBcccccBcccBcDDDDDDDDDDDDDDDDDD +@HELIUM_000100422_612GNAAXX:7:120:16005:7955#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=98; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=64; seq_ab_match=61; sminR=40.0; seq_b_mismatch=2; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.8939393939; seq_a_deletion=0; seq_b_single=44; score=231.880428082; seq_a_insertion=0; mode=alignment; seq_length_ori=152; head_quality=67.0; avg_quality=77.3289473684; sminL=40.0; seq_a_single=44; score_norm=3.62313168878; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagccgaaactcaaaggacttggcggtgctttacatccct ++ +ddddddddddddddddd~~~~b~~~~~~~~~b~~~~~~~~~~~z~~~~~~~~~~{~~~~~~~`~~~~~~~~~~~~~~~~~~ddddcdddddddddddd +@HELIUM_000100422_612GNAAXX:7:115:3396:14235#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=61; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.1052631579; seq_a_deletion=0; seq_b_single=45; score=235.745732131; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=76.6535947712; sminL=40.0; seq_a_single=45; score_norm=3.74199574811; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +ddcdddddadddddcddd~~~~~~b~~~~~~~~~}~}~ccc~|~~~~{~~~~~~~s~~~~~~~~~~~~~~~~~~~b~~~~~ddddddddddcdcddddd +@HELIUM_000100422_612GNAAXX:7:32:15197:9940#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=66.9; seq_b_deletion=0; mid_quality=78.3656716418; seq_a_deletion=0; seq_b_single=46; score=231.86950825; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.1; avg_quality=76.8896103896; sminL=40.0; seq_a_single=46; score_norm=3.73983077822; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddcaddddddddddddcdd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~wt}}~~~~~{~~~~~~~~~~~~~~~b~~~~~~bd`ddddcddddddcddddd +@HELIUM_000100422_612GNAAXX:7:7:17828:13825#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=56; sminR=40.0; seq_b_mismatch=3; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=1; mid_quality=77.5223880597; seq_a_deletion=0; seq_b_single=46; score=187.930205125; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.1558441558; sminL=40.0; seq_a_single=46; score_norm=3.03113234072; seq_b_insertion=1; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddbddddddddacbaa[b~b~~~~~~~~~~~~~a~~~~~~~~~~~~b~~~~~~~~~~~b~~~~~~~b}~~~~~~~~~~~_ddddddddddddccdddcd +@HELIUM_000100422_612GNAAXX:7:53:2128:13141#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=15a_F730814; experiment=wolf_diet; forward_tag=gaagtag; reverse_tag=gaagtag; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.947761194; seq_a_deletion=0; seq_b_single=46; score=247.978630411; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.9; avg_quality=77.3896103896; sminL=40.0; seq_a_single=46; score_norm=3.99965532921; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddddddddddddd^dad~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddcddddddddddcddddd +@HELIUM_000100422_612GNAAXX:7:72:7057:20448#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.0298507463; seq_a_deletion=0; seq_b_single=46; score=247.94833093; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.4675324675; sminL=40.0; seq_a_single=46; score_norm=3.9991666279; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddeddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddebdddd +@HELIUM_000100422_612GNAAXX:7:10:9237:10532#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=57; sminR=40.0; seq_b_mismatch=3; seq_a_mismatch=2; tail_quality=66.9; seq_b_deletion=0; mid_quality=75.9402985075; seq_a_deletion=0; seq_b_single=46; score=207.90944986; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.7; avg_quality=74.7532467532; sminL=40.0; seq_a_single=46; score_norm=3.35337822355; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaataattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +cSc`ddddddddddda_\`~~~~~~~~~~~~~~~~N~~\~~~~~~~~~~~~~~~Y~~jopppx|~|o~~~~~~b~~b~~~~ddddadddcadddada]V] +@HELIUM_000100422_612GNAAXX:7:16:16055:12835#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.7462686567; seq_a_deletion=0; seq_b_single=46; score=239.868244499; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.2207792208; sminL=40.0; seq_a_single=46; score_norm=3.86884265322; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +db`dddcdddddddddddc~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~dddddddddddddcddddd +@HELIUM_000100422_612GNAAXX:7:17:16877:9760#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=3; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.3731343284; seq_a_deletion=0; seq_b_single=46; score=223.899159093; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.8961038961; sminL=40.0; seq_a_single=46; score_norm=3.61127675956; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddddddddddcddddddd~b~~~~~~~~~~~~~a~~~~~~b~~~{~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:47:8320:8571#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=61; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.4586466165; seq_a_deletion=0; seq_b_single=45; score=235.809300513; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=66.9; avg_quality=76.954248366; sminL=40.0; seq_a_single=45; score_norm=3.74300477004; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddddddddddddddddd~~|~~~~~b~~~~~~~~~~~~~~~~~~~~~uuy~~~~~~~~~~~|~~~~~x~~~~~~~~~~b~daddddadddddd\ddda +@HELIUM_000100422_612GNAAXX:7:6:6617:2744#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.7593984962; seq_a_deletion=0; seq_b_single=45; score=235.875132744; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.2222222222; sminL=40.0; seq_a_single=45; score_norm=3.7440497261; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +eddddd`ddddddddcbd~b~~~~b~~~~~~~~~~~~|~~~~~~~~y~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddcbdd +@HELIUM_000100422_612GNAAXX:7:12:9609:14740#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.1; seq_b_deletion=0; mid_quality=78.4701492537; seq_a_deletion=0; seq_b_single=46; score=231.833538451; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.1; avg_quality=76.9935064935; sminL=40.0; seq_a_single=46; score_norm=3.73925062018; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dcddddddddddcdddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~zs~}~~~~~}~~~~~~~~~~~~~~~~~~b~~~bdddddddcdddddeddddd +@HELIUM_000100422_612GNAAXX:7:21:2093:4412#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=3; tail_quality=67.0; seq_b_deletion=0; mid_quality=76.1428571429; seq_a_deletion=0; seq_b_single=45; score=227.763131817; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=74.9477124183; sminL=40.0; seq_a_single=45; score_norm=3.61528780662; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +eddddddddddddddddd~b~~~~~~~~~~~~~}~~`~~~~~~~~~~~~~Z{~~|{~~~~{~|~|~~~~~}~~~}|~~}}}DDDDDDDD``U`^\[ddd +@HELIUM_000100422_612GNAAXX:7:18:5240:1587#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=2; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.3880597015; seq_a_deletion=0; seq_b_single=46; score=223.905608015; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.9090909091; sminL=40.0; seq_a_single=46; score_norm=3.61138077443; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddddddddddddddddd~}~~~~~~~~~~~~}~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~bdddddddedddddcddddd +@HELIUM_000100422_612GNAAXX:7:88:15797:19191#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=58; sminR=40.0; seq_b_mismatch=4; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.1492537313; seq_a_deletion=0; seq_b_single=46; score=215.88300628; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.7012987013; sminL=40.0; seq_a_single=46; score_norm=3.48198397226; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddddddddddddddcddc~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~`~~~~b~~b~~~~ddeddddddddddcddddd +@HELIUM_000100422_612GNAAXX:7:47:11864:1343#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.2857142857; seq_a_deletion=0; seq_b_single=45; score=251.976310174; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.6797385621; sminL=40.0; seq_a_single=45; score_norm=3.99962397102; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +ddddddddddddddbdcd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddcddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:43:8022:8427#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.5970149254; seq_a_deletion=0; seq_b_single=46; score=231.869859511; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.0909090909; sminL=40.0; seq_a_single=46; score_norm=3.73983644372; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dedcddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~b~~~~dddddddddddddcddddd +@HELIUM_000100422_612GNAAXX:7:95:11283:2557#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=4; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=58; seq_ab_match=57; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=0; tail_quality=93.0; seq_b_deletion=50; mid_quality=92.2368421053; seq_a_deletion=50; seq_b_single=0; score=223.999078974; seq_a_insertion=0; mode=alignment; seq_length_ori=58; head_quality=93.0; avg_quality=92.5; sminL=40.0; seq_a_single=0; score_norm=3.86205308576; seq_b_insertion=0; +tttt ++ +~~~~ +@HELIUM_000100422_612GNAAXX:7:67:5288:13403#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.0298507463; seq_a_deletion=0; seq_b_single=46; score=247.999078967; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.1; avg_quality=77.474025974; sminL=40.0; seq_a_single=46; score_norm=3.99998514464; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddddddddcdddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddeddddd +@HELIUM_000100422_612GNAAXX:7:7:11998:4462#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=59; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=4; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.2105263158; seq_a_deletion=0; seq_b_single=45; score=219.864332815; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=76.7450980392; sminL=40.0; seq_a_single=45; score_norm=3.48991004469; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtagtactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +ddddddddddddddcddd~b~~~~~~~~~~~~b~~~~~~~b~~~~~~~G~}}~~}}~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddcdccdd +@HELIUM_000100422_612GNAAXX:7:116:12598:9964#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=53; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=8; tail_quality=66.7; seq_b_deletion=0; mid_quality=77.0671641791; seq_a_deletion=0; seq_b_single=46; score=175.843125508; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=75.7402597403; sminL=40.0; seq_a_single=46; score_norm=2.83617944368; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddddcdddddddbddda`~~~~~~~~~~~~~~~~~~b~~~b~~~~b~~~~~b~b~{~~~~~~b~~~~~~~~~~~b~~~bddddddedddddddddddd +@HELIUM_000100422_612GNAAXX:7:111:18277:17779#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.8646616541; seq_a_deletion=0; seq_b_single=45; score=235.877819976; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.3137254902; sminL=40.0; seq_a_single=45; score_norm=3.74409238057; seq_b_insertion=0; +ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaatagcctgaaactcaaaggacttggcggtgctttacatccct ++ +ddddddddddddbdddcd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~_~~~~~~~~~~~~b~~~~~dddddddedddddddddd +@HELIUM_000100422_612GNAAXX:7:85:13257:14594#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.5298507463; seq_a_deletion=0; seq_b_single=46; score=231.92296471; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.0324675325; sminL=40.0; seq_a_single=46; score_norm=3.7406929792; seq_b_insertion=0; +ttagccctaaacacaaataattacacaaacaaaattgttcaccagagtactagcggcaacagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddddddedddddddddd~~~~~~~~~~~~~~~~~~~~~~~_~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~addccddadddddcaddddd +@HELIUM_000100422_612GNAAXX:7:17:11935:17343#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.0902255639; seq_a_deletion=0; seq_b_single=45; score=243.983570342; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.5098039216; sminL=40.0; seq_a_single=45; score_norm=3.87275508479; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddddddddddddddddd~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:38:9654:18700#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.0902255639; seq_a_deletion=0; seq_b_single=45; score=243.950053136; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.5098039216; sminL=40.0; seq_a_single=45; score_norm=3.87222306566; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +ddddddddddddddeddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~~~~~~~~~~~dcddddddddddcddddc +@HELIUM_000100422_612GNAAXX:7:101:11720:19906#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=57; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=4; tail_quality=66.9; seq_b_deletion=0; mid_quality=76.9104477612; seq_a_deletion=0; seq_b_single=46; score=207.841888471; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=75.6168831169; sminL=40.0; seq_a_single=46; score_norm=3.35228852373; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddc_ddddddcdddddd\d~~~~~~~~~~~~~~~~]~~|~hsp~~{~~z{t}~~qs}~b~~}~~~~}~x~~~~b~~c~~~_ddddcd^ccbbdd^ddddd +@HELIUM_000100422_612GNAAXX:7:42:12961:8650#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.1052631579; seq_a_deletion=0; seq_b_single=45; score=243.985409859; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.522875817; sminL=40.0; seq_a_single=45; score_norm=3.87278428347; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddddddddddddddddd~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddcddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:36:10388:10324#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=15a_F730814; experiment=wolf_diet; forward_tag=gaagtag; reverse_tag=gaagtag; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.8208955224; seq_a_deletion=0; seq_b_single=46; score=239.950587748; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.2857142857; sminL=40.0; seq_a_single=46; score_norm=3.87017077012; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddddddddddddddddd~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddeddddd +@HELIUM_000100422_612GNAAXX:7:66:11860:20419#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.1; seq_b_deletion=0; mid_quality=78.5671641791; seq_a_deletion=0; seq_b_single=46; score=239.911387587; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.1; avg_quality=77.0779220779; sminL=40.0; seq_a_single=46; score_norm=3.86953850946; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddcdddddeddddddddad~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~}~~~~~~~~~~~~~~~b~~~|ddcddddddddddNddddd +@HELIUM_000100422_612GNAAXX:7:3:17077:6562#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=3; tail_quality=66.6; seq_b_deletion=0; mid_quality=77.6567164179; seq_a_deletion=0; seq_b_single=46; score=223.876050008; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.0; avg_quality=76.1818181818; sminL=40.0; seq_a_single=46; score_norm=3.61090403239; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactgccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddcdddddddddddadab~~~~~~~~~~~~~~~~~~~~~~~~~~~~zz~zF~a~~~~~~~}~~~~~~~~~~~~~~au~~~ccccccccccccacccccc +@HELIUM_000100422_612GNAAXX:7:18:11865:6418#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.3157894737; seq_a_deletion=0; seq_b_single=45; score=251.959188225; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.7058823529; sminL=40.0; seq_a_single=45; score_norm=3.99935219405; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddcdddeddddddd +@HELIUM_000100422_612GNAAXX:7:19:4311:13343#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=58; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=4; tail_quality=67.0; seq_b_deletion=0; mid_quality=77.7969924812; seq_a_deletion=0; seq_b_single=45; score=211.837822462; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=76.385620915; sminL=40.0; seq_a_single=45; score_norm=3.36250511845; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactacgagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dbddcddddcdd^ddddd~~~~~~~~~~~~~b~~~~~~~~~~~~~~~~~~~{Z{~~~_~~y{~~~}~~~|~~~~~b~~~b~bcdddcdddddddddccc +@HELIUM_000100422_612GNAAXX:7:30:1800:7833#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=15a_F730814; experiment=wolf_diet; forward_tag=gaagtag; reverse_tag=gaagtag; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=52; sminR=40.0; seq_b_mismatch=10; seq_a_mismatch=0; tail_quality=65.6; seq_b_deletion=0; mid_quality=59.9104477612; seq_a_deletion=0; seq_b_single=46; score=167.712615339; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=35.0; avg_quality=58.6623376623; sminL=40.0; seq_a_single=46; score_norm=2.70504218289; seq_b_insertion=0; +ttagccctaaacacaagacattaatataacgagattaatcgacagagtactaccggctatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +DDDDDDDDDDDDDDDDDDDccBccccccccBcBccccBBccBcccccccc~u~v}[wJ~~Q|~~~~~~~~~~~~a~~~~t~Z^ccc`c]bbc_[V_[`[` +@HELIUM_000100422_612GNAAXX:7:16:19299:8684#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.5; seq_a_deletion=0; seq_b_single=46; score=231.845804746; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.0064935065; sminL=40.0; seq_a_single=46; score_norm=3.73944846365; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +cdccddddeddddddadac~~~~~~~~~~~~~~~~~~~~~~~~|~~~~~~~~~~~~~~~~~~~~~~~~`~~~~~~~b~~~~dddddddddddddcddddd +@HELIUM_000100422_612GNAAXX:7:54:14618:14353#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.6865671642; seq_a_deletion=0; seq_b_single=46; score=239.8562981; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.1688311688; sminL=40.0; seq_a_single=46; score_norm=3.86864996935; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddcedddddddddddddde~~~~~~~~~~~~~~~~~~~~~~~~~~~~~z}|z~~~~~y~~~~~~~~~~~~~~~~~~b~~~~ddddddcddddddcddddd +@HELIUM_000100422_612GNAAXX:7:108:9222:18258#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=2; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.5; seq_a_deletion=0; seq_b_single=46; score=231.857703869; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.2; avg_quality=77.0194805195; sminL=40.0; seq_a_single=46; score_norm=3.73964038498; seq_b_insertion=0; +ctagccttaaacacaaatagttatgcagacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddccddddddddddddddd~~~~~~~~~~~~~~~~~~~v~~~~~}~}}~~~~~`~~~~~~~~~~b~~~~~~~~~~~~~~~~dcddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:74:4783:20479#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=77.9029850746; seq_a_deletion=0; seq_b_single=46; score=239.87314657; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.487012987; sminL=40.0; seq_a_single=46; score_norm=3.86892171887; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddaddddddbddddZd`d~b~~~~~~~~~~~~~~~~~}~~~~~~~~~~~~~~~|z~~~~~xq~~~}~r~~~~~~~~~~yqcbbddWdadddddWddcda +@HELIUM_000100422_612GNAAXX:7:38:3005:20881#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=3; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.5939849624; seq_a_deletion=0; seq_b_single=45; score=227.901935995; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.0784313725; sminL=40.0; seq_a_single=45; score_norm=3.61749104754; seq_b_insertion=0; +ttagccctaaacatgaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +ddddddddddddddaedd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~a~~~~~~~|~~~~~~b~~b~~}~~dbdddcdddddbdddddd +@HELIUM_000100422_612GNAAXX:7:4:10139:2389#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=66.5; seq_b_deletion=0; mid_quality=78.4962406015; seq_a_deletion=0; seq_b_single=45; score=251.802206044; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=66.9; avg_quality=76.954248366; sminL=40.0; seq_a_single=45; score_norm=3.99686041339; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +daddaddddbdddddddc~~~~~~~~~{~~~~~~~~zxy~~|~~~~ru~~~~~~~~~~~~~~~~~~{~~p~~~~~~~~~~~ddddddddddabaP`ddd +@HELIUM_000100422_612GNAAXX:7:75:9674:16966#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=4; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=58; seq_ab_match=57; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=0; tail_quality=93.0; seq_b_deletion=50; mid_quality=92.2631578947; seq_a_deletion=50; seq_b_single=0; score=224.000075421; seq_a_insertion=0; mode=alignment; seq_length_ori=58; head_quality=93.0; avg_quality=92.5172413793; sminL=40.0; seq_a_single=0; score_norm=3.86207026589; seq_b_insertion=0; +tttt ++ +~~~~ +@HELIUM_000100422_612GNAAXX:7:59:15367:8376#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=15a_F730814; experiment=wolf_diet; forward_tag=gaagtag; reverse_tag=gaagtag; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.0149253731; seq_a_deletion=0; seq_b_single=46; score=247.996374666; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.4545454545; sminL=40.0; seq_a_single=46; score_norm=3.99994152687; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddddddddddddddddad~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddeddddd +@HELIUM_000100422_612GNAAXX:7:29:1328:13185#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=59; sminR=40.0; seq_b_mismatch=4; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.3007518797; seq_a_deletion=0; seq_b_single=45; score=223.94591652; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=76.8235294118; sminL=40.0; seq_a_single=45; score_norm=3.55469708763; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddddddddddddedadd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~W[~~~~~~~~~~~~~~~~~~~~~~~b~~~b~dddcdddddddcdddddd +@HELIUM_000100422_612GNAAXX:7:30:16081:4486#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=62; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.0902255639; seq_a_deletion=0; seq_b_single=45; score=243.950667893; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.5098039216; sminL=40.0; seq_a_single=45; score_norm=3.87223282369; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +dddaddddddddddeddd~~~c~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddcddddddddddd +@HELIUM_000100422_612GNAAXX:7:104:10274:7999#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.2556390977; seq_a_deletion=0; seq_b_single=45; score=251.962499638; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=66.9; avg_quality=77.6470588235; sminL=40.0; seq_a_single=45; score_norm=3.99940475616; seq_b_insertion=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +ccdddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddd^ddddddddddd +@HELIUM_000100422_612GNAAXX:7:14:7706:12338#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=77.6194029851; seq_a_deletion=0; seq_b_single=46; score=223.864093999; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.7; avg_quality=76.2207792208; sminL=40.0; seq_a_single=46; score_norm=3.61071119354; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +dddadddcddddddddc^d~~~~~~~~~~~~~~~~~~~P~~~~~~~~~}}~vm~qZyw~~~~x~~~~~~~~~~~b~~~~~~dddddaccdddddcddddd diff -r 000000000000 -r aca6a8c9cf83 test-data/output_ngsfilter_unidentified.fastq --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/output_ngsfilter_unidentified.fastq Wed Apr 12 17:38:00 2017 -0400 @@ -0,0 +1,44 @@ +@HELIUM_000100422_612GNAAXX:7:1:9007:3289#0/2_CONS_SUB_SUB_CMP error=Cannot assign sequence to a sample; forward_score=72.0; forward_tag=atctctc; reverse_tag=atctctc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.5671641791; seq_a_deletion=0; seq_b_single=46; score=231.83380127; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.0649350649; sminL=40.0; seq_a_single=46; score_norm=3.7392548592; seq_b_insertion=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +ddddddddddddddcddbd~~~~~~~~~~~~~~~~~~~}~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~bddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:1:8849:9880#0/2_PairEnd sminR=40.0; pairend_limit=108; tail_quality=64.3; mid_quality=37.8724489796; ali_dir=left; score=25.9064763088; mode=joined; error=No primer match; head_quality=33.0; avg_quality=38.8703703704; sminL=40.0; +ggacgggagagggggggggggggggaaggggggggttttggggggggggggccgttttaaaaaaaaaaaaaaaacaacgtgacatggcacagccgtcacaactgtcagctgacgaccactggcagtgagtctactctcctattttgttttttttttcaagcagaagacggcatacgatatcggtctcggcattcctgctgaaccgctcttccgatc ++ +BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB_`X`b[`T[ZWHZTWU]]b]]TZ^__\^`\OOOOO^`^Z^_ba_bbbbbb +@HELIUM_000100422_612GNAAXX:7:1:8649:5297#0/2_CONS ali_length=104; direction=right; seq_ab_match=104; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=93.0; seq_b_deletion=4; mid_quality=93.0; seq_a_deletion=4; seq_b_single=0; score=415.823014771; seq_a_insertion=0; mode=alignment; error=No primer match; head_quality=93.0; avg_quality=93.0; sminL=40.0; seq_a_single=0; score_norm=3.99829821895; seq_b_insertion=0; +ccagcgagatcccattgagtctctgcacctatcctttttttcgttttctgaatctttgtttggaaaaaaacaggatttggctcaggattgcccgatctcgctgg ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@HELIUM_000100422_612GNAAXX:7:1:8702:15835#0/2_CONS ali_length=85; direction=left; seq_ab_match=85; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=86.8828828829; seq_a_deletion=0; seq_b_single=23; score=339.959438284; seq_a_insertion=0; mode=alignment; error=No primer match; head_quality=67.0; avg_quality=83.8473282443; sminL=40.0; seq_a_single=23; score_norm=3.99952280334; seq_b_insertion=0; +cccatcgctcagatatccgttgccgagagtcgtgtagattatatagaattgcaactcggggtgcggccagcaagccagccacatcccctcgttaggcacagtgttccttgacgccttcggtgagcgatggg ++ +ddddddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~{~~~~~~~~ddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:1:8613:9165#0/2_CONS ali_length=101; direction=left; seq_ab_match=99; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=74.8; seq_b_deletion=0; mid_quality=92.4105263158; seq_a_deletion=0; seq_b_single=7; score=387.825112777; seq_a_insertion=0; mode=alignment; error=No primer match; head_quality=74.8; avg_quality=89.347826087; sminL=40.0; seq_a_single=7; score_norm=3.83985260176; seq_b_insertion=0; +ccagagcgagtgggcaatcctgagccaaatctttatttttagaaaaacaaaagggcttcagaaagcaaaaataaacataaaggataggtgcagagactcaatggactcgctctgg ++ +ddddddd~~~~b~~~~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddd +@HELIUM_000100422_612GNAAXX:7:1:8670:8750#0/2_CONS ali_length=90; direction=right; seq_ab_match=90; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=93.0; seq_b_deletion=18; mid_quality=93.0; seq_a_deletion=18; seq_b_single=0; score=359.914979278; seq_a_insertion=0; mode=alignment; error=No primer match; head_quality=93.0; avg_quality=93.0; sminL=40.0; seq_a_single=0; score_norm=3.99905532531; seq_b_insertion=0; +ccgaggcttactaggattagataccctattatttttgaatgttaatgtttgtttgcttgagtagtattagttatgttcttgaaagcctcg ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@HELIUM_000100422_612GNAAXX:7:1:8732:6209#0/2_PairEnd sminR=40.0; pairend_limit=108; tail_quality=57.9; mid_quality=36.5408163265; ali_dir=right; score=15.9727840143; mode=joined; error=No primer match; head_quality=33.0; avg_quality=37.3657407407; sminL=40.0; +gctctacagaggcgggggggggggagaaggggaaatcatggggggggggggcgacatctaaaaacatacatacaacccacacctcctaccacacagctcccagcgcaaagatagatcatgatggtaagtagaggcaaatgagatcatctatttttttcaaagagaagacggcatacgatatcggtctcggcattcctgctaaccgctcttccgatc ++ +BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBa[]aa_aaaa]aa`]a\[aZ`\ZcMWR]Z````` +@HELIUM_000100422_612GNAAXX:7:1:8858:2566#0/2_PairEnd sminR=40.0; pairend_limit=108; tail_quality=65.0; mid_quality=40.6581632653; ali_dir=left; score=15.7312871104; mode=joined; error=No primer match; head_quality=33.0; avg_quality=41.4305555556; sminL=40.0; +ggctcggagagagggggggggggggggaggggggggatccgggggggggggggtcttacaaaaaaacacaaaacacataaccactctcacacccacactcgcacacccggatgtggttgtggtgtgcgcgtggatggtgtggtgtggtttttttttcaagcagaagacggcatacgagatcggtctcggcattcctgctgaaccgctcttccgatc ++ +BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBa_a_a]aaaa_`Y`_W]Y[S]]aaaabbbbbcbbbbbbbbbbbbb`bbbbbbbbbbbbbb +@HELIUM_000100422_612GNAAXX:7:1:8644:15229#0/2_CONS ali_length=106; direction=left; seq_ab_match=103; sminR=40.0; seq_b_mismatch=2; seq_a_mismatch=1; tail_quality=83.8; seq_b_deletion=0; mid_quality=90.8; seq_a_deletion=0; seq_b_single=2; score=399.617128202; seq_a_insertion=0; mode=alignment; error=No primer match; head_quality=87.8; avg_quality=89.8909090909; sminL=40.0; seq_a_single=2; score_norm=3.76997290756; seq_b_insertion=0; +cacacgctctccattgagtctctgcacctatcctttttgattttcgctttctgaatctttgtttgttttcggaaaacgtgatttggctcaggattgcccagagcgtgtgg ++ +dd~~~~~~~~~~~~~~~~~~~~~~~~{~~~~b~~~~~~~~~{|~~~~s|~v~~|p~~~~~~~~~~~u~qy~a~~~~~~~x~|~~~~z{~|~o|~{yvwwy~~|~~[~~cb +@HELIUM_000100422_612GNAAXX:7:1:8613:5665#0/2_CONS ali_length=90; direction=right; seq_ab_match=89; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=0; tail_quality=90.2; seq_b_deletion=18; mid_quality=93.0; seq_a_deletion=18; seq_b_single=0; score=351.879189487; seq_a_insertion=0; mode=alignment; error=No primer match; head_quality=93.0; avg_quality=92.6888888889; sminL=40.0; seq_a_single=0; score_norm=3.90976877208; seq_b_insertion=0; +ccttcctggggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaccaggaagg ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~ +@HELIUM_000100422_612GNAAXX:7:1:8823:6682#0/2_PairEnd sminR=40.0; pairend_limit=108; tail_quality=64.9; mid_quality=41.693877551; ali_dir=right; score=15.9839512414; mode=joined; error=No primer match; head_quality=33.0; avg_quality=42.3657407407; sminL=40.0; +gatcgggagagcgtcgtgtaggggaagagggtagatctcggggggggccgtatcattaaaaaaaaaaacaaacatcagcacgaagcacatgacaccgcctagctatcaatcttgagatgagatcagacatctgtttgttttcttttgtttttttttcaagcagaagacggcatacgatatcggtctcggcattcctgctgaaccgctcttccgatc ++ +BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB_aa]a`]^S``aabaab^bbbbab_c``abbbbbbbcbbbbbbbbbbbbbbbbbbbbbbbbbbab diff -r 000000000000 -r aca6a8c9cf83 test-data/output_obiannotate.fasta --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/output_obiannotate.fasta Wed Apr 12 17:38:00 2017 -0400 @@ -0,0 +1,172 @@ +>HELIUM_000100422_612GNAAXX:7:119:14871:19157#0/2_CONS_SUB_SUB_CMP merged_sample={'29a_F260619': 1}; seq_rank=1; count=1; seq_length=100; +ttagccctaaacacaagtaattattataacaaaatcattcgccagagtactaccggcaat +agctcaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:108:5640:3823#0/2_CONS_SUB_SUB_CMP merged_sample={'29a_F260619': 1}; seq_rank=2; count=1; seq_length=100; +ttagccctaaacacaagtaattaatataacaaaattgttcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:97:14311:19299#0/2_CONS_SUB_SUB_CMP merged_sample={'29a_F260619': 1}; seq_rank=3; count=1; seq_length=100; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaag +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:22:8540:14708#0/2_CONS_SUB_SUB merged_sample={'29a_F260619': 30, '15a_F730814': 31}; count=61; seq_length=100; seq_rank=4; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:57:18459:16145#0/2_CONS_SUB_SUB_CMP merged_sample={'26a_F040644': 64}; seq_rank=5; count=64; seq_length=99; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca +gcctgaaactcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:89:10281:5930#0/2_CONS_SUB_SUB_CMP merged_sample={'29a_F260619': 3}; seq_rank=6; count=3; seq_length=100; +ttagccctaaacacaaataattacacaaacaaaattgttcaccagagtactagcggcaac +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:29:15520:18035#0/2_CONS_SUB_SUB merged_sample={'29a_F260619': 2}; seq_rank=7; count=2; seq_length=100; +ttagccctaaacacaagtaattattataacaaaattattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:6:9274:14951#0/2_CONS_SUB_SUB merged_sample={'13a_F730603': 46}; count=46; seq_length=100; seq_rank=8; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:24:16230:12703#0/2_CONS_SUB_SUB_CMP merged_sample={'15a_F730814': 1}; seq_rank=9; count=1; seq_length=100; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttatcccctt +>HELIUM_000100422_612GNAAXX:7:4:4214:9434#0/2_CONS_SUB_SUB merged_sample={'13a_F730603': 1}; seq_rank=10; count=1; seq_length=100; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactacaggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:13:6954:13039#0/2_CONS_SUB_SUB_CMP merged_sample={'26a_F040644': 1}; seq_rank=11; count=1; seq_length=99; +ttagccctaaacataaacattcaataaacaagaatgttcgccagaggactactagcaata +gcttaaaactcaaaggacttggcggtgctttatatccct +>HELIUM_000100422_612GNAAXX:7:38:6201:12003#0/2_CONS_SUB_SUB_CMP merged_sample={'26a_F040644': 1}; seq_rank=12; count=1; seq_length=99; +ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaata +gcttaaaactcaaagaactgggcggtgctttatatcccg +>HELIUM_000100422_612GNAAXX:7:115:17402:4174#0/2_CONS_SUB_SUB_CMP merged_sample={'29a_F260619': 1, '15a_F730814': 1}; seq_rank=13; count=2; seq_length=100; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactacctgcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:78:7151:19928#0/2_CONS_SUB_SUB merged_sample={'26a_F040644': 1}; seq_rank=14; count=1; seq_length=99; +ttagccctaaacataaacattcaataaacgagaatgttcgccagtgtacttctagcaaca +ggctgaaactcaaaggacttggcggtggtttacatccct +>HELIUM_000100422_612GNAAXX:7:38:10813:16867#0/2_CONS_SUB_SUB merged_sample={'26a_F040644': 2}; seq_rank=15; count=2; seq_length=99; +ttagccctaaacataaacattcaataaacaagaatgtttgccagagtactactagcaaca +gcctgaaactcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:13:5742:9859#0/2_CONS_SUB_SUB merged_sample={'29a_F260619': 7}; seq_rank=16; count=7; seq_length=4; +tttt +>HELIUM_000100422_612GNAAXX:7:16:16357:19948#0/2_CONS_SUB_SUB merged_sample={'26a_F040644': 1}; seq_rank=17; count=1; seq_length=99; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca +gcctgaaactcacaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:107:3570:18147#0/2_CONS_SUB_SUB_CMP merged_sample={'29a_F260619': 1}; seq_rank=18; count=1; seq_length=100; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat +agcataaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:114:4511:17596#0/2_CONS_SUB_SUB_CMP merged_sample={'29a_F260619': 1}; seq_rank=19; count=1; seq_length=100; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtgctaccggcaat +agcttaaaactcaaaggacttggcggtgctttatacgctt +>HELIUM_000100422_612GNAAXX:7:70:11798:2668#0/2_CONS_SUB_SUB merged_sample={'15a_F730814': 2}; seq_rank=20; count=2; seq_length=100; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccttt +>HELIUM_000100422_612GNAAXX:7:29:18416:16620#0/2_CONS_SUB_SUB_CMP merged_sample={'29a_F260619': 1}; seq_rank=21; count=1; seq_length=100; +ttagccctaaacacaagtaattattataacaaaattattcgccagagttctaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctg +>HELIUM_000100422_612GNAAXX:7:120:1900:13295#0/2_CONS_SUB_SUB_CMP merged_sample={'15a_F730814': 1}; seq_rank=22; count=1; seq_length=100; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactacgcgcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:76:10822:18722#0/2_CONS_SUB_SUB merged_sample={'26a_F040644': 1}; seq_rank=23; count=1; seq_length=99; +ttagccctaaacataaacattcaataaacacgaatgttcgccagagtactactagcaaca +gcctgaaactcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:76:8491:8900#0/2_CONS_SUB_SUB_CMP merged_sample={'15a_F730814': 1}; seq_rank=24; count=1; seq_length=100; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtgctacctgcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:9:10358:4089#0/2_CONS_SUB_SUB_CMP merged_sample={'13a_F730603': 1}; seq_rank=25; count=1; seq_length=100; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactacaggcaat +agctcaaaactcaaagaacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:59:11971:12063#0/2_CONS_SUB_SUB merged_sample={'29a_F260619': 1}; seq_rank=26; count=1; seq_length=100; +ttagccctaaacacaagtaattaatataacaaaattattcgtcagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:111:19168:18517#0/2_CONS_SUB_SUB merged_sample={'15a_F730814': 1}; seq_rank=27; count=1; seq_length=100; +ttagccctaaacacaagtaattaatataacaaaattatccgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:41:9316:2256#0/2_CONS_SUB_SUB merged_sample={'29a_F260619': 1}; seq_rank=28; count=1; seq_length=100; +ttagccctaaacacaagaaattaatataacaaaattattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:33:9900:9541#0/2_CONS_SUB_SUB merged_sample={'13a_F730603': 1}; seq_rank=29; count=1; seq_length=100; +ctagccctaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:95:1688:9598#0/2_CONS_SUB_SUB_CMP merged_sample={'26a_F040644': 1}; seq_rank=30; count=1; seq_length=99; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtgatactagcaaca +gcctgaaactcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:55:16528:11418#0/2_CONS_SUB_SUB merged_sample={'13a_F730603': 1}; seq_rank=31; count=1; seq_length=100; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcact +agcttacaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:19:6313:16567#0/2_CONS_SUB_SUB_CMP merged_sample={'26a_F040644': 1}; seq_rank=32; count=1; seq_length=99; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca +gcctgaaactcaaagggcttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:111:8478:1127#0/2_CONS_SUB_SUB_CMP merged_sample={'26a_F040644': 1}; seq_rank=33; count=1; seq_length=99; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca +gcctggaacgcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:81:15726:2345#0/2_CONS_SUB_SUB_CMP merged_sample={'26a_F040644': 1}; seq_rank=34; count=1; seq_length=99; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca +gcctgaaactcaaagcactcggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:7:11110:17924#0/2_CONS_SUB_SUB merged_sample={'15a_F730814': 1}; seq_rank=35; count=1; seq_length=100; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtcataccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:89:9710:20652#0/2_CONS_SUB_SUB merged_sample={'26a_F040644': 1}; seq_rank=36; count=1; seq_length=99; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca +gcctgaaactcaaaggacttggcggtgctttatatccct +>HELIUM_000100422_612GNAAXX:7:71:17473:7401#0/2_CONS_SUB_SUB_CMP merged_sample={'15a_F730814': 1}; seq_rank=37; count=1; seq_length=100; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggtaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:115:3625:10051#0/2_CONS_SUB_SUB merged_sample={'13a_F730603': 1}; seq_rank=38; count=1; seq_length=5; +caata +>HELIUM_000100422_612GNAAXX:7:99:8183:13912#0/2_CONS_SUB_SUB merged_sample={'13a_F730603': 1}; seq_rank=39; count=1; seq_length=100; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctc +>HELIUM_000100422_612GNAAXX:7:22:1798:3790#0/2_CONS_SUB_SUB_CMP merged_sample={'13a_F730603': 1}; seq_rank=40; count=1; seq_length=100; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtgctaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:115:14354:10118#0/2_CONS_SUB_SUB_CMP merged_sample={'26a_F040644': 1}; seq_rank=41; count=1; seq_length=99; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactcgcaaca +gcctgaaactcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:103:3594:15996#0/2_CONS_SUB_SUB merged_sample={'13a_F730603': 1}; seq_rank=42; count=1; seq_length=100; +ctagccttaaacacaaatagttatgcaaacacaactattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:105:14135:6905#0/2_CONS_SUB_SUB merged_sample={'29a_F260619': 1}; seq_rank=43; count=1; seq_length=100; +ttagccctaaacacaagtaattaatataacaaaattattcaccagagtactagcggcaac +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:76:18268:14980#0/2_CONS_SUB_SUB_CMP merged_sample={'29a_F260619': 1}; seq_rank=44; count=1; seq_length=100; +ttacccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:25:7789:3090#0/2_CONS_SUB_SUB_CMP merged_sample={'29a_F260619': 1}; seq_rank=45; count=1; seq_length=100; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactacaggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:42:19757:3800#0/2_CONS_SUB_SUB_CMP merged_sample={'15a_F730814': 1}; seq_rank=46; count=1; seq_length=99; +ttagccctaaacacaagtaattaatatacaaaattattcgccagagtactaccggcaata +gcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:116:14244:17926#0/2_CONS_SUB_SUB merged_sample={'26a_F040644': 1}; seq_rank=47; count=1; seq_length=99; +ttagccctaaacataaacattcaataaacaagaatgttcgcaagagtactactagcaaca +gcctgaaactcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:72:12959:5736#0/2_CONS_SUB_SUB_CMP merged_sample={'26a_F040644': 1}; seq_rank=48; count=1; seq_length=99; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca +gcctgaaactcaaaggacttggcggtgctttacacccct +>HELIUM_000100422_612GNAAXX:7:7:15538:8325#0/2_CONS_SUB_SUB_CMP merged_sample={'26a_F040644': 1}; seq_rank=49; count=1; seq_length=99; +ttagccctaaacataaacattcaataaacaagaatgtcggccagagtactactagcaaca +gcatgaaactcaaagaactgggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:120:16005:7955#0/2_CONS_SUB_SUB_CMP merged_sample={'26a_F040644': 1}; seq_rank=50; count=1; seq_length=98; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca +gccgaaactcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:10:9237:10532#0/2_CONS_SUB_SUB_CMP merged_sample={'29a_F260619': 1}; seq_rank=51; count=1; seq_length=100; +ttagccctaaacacaagtaattaatataacaaaataattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:7:11998:4462#0/2_CONS_SUB_SUB merged_sample={'26a_F040644': 1}; seq_rank=52; count=1; seq_length=99; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtagtactagcaaca +gcctgaaactcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:111:18277:17779#0/2_CONS_SUB_SUB_CMP merged_sample={'26a_F040644': 1}; seq_rank=53; count=1; seq_length=99; +ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaata +gcctgaaactcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:3:17077:6562#0/2_CONS_SUB_SUB_CMP merged_sample={'15a_F730814': 1}; seq_rank=54; count=1; seq_length=100; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactgccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:19:4311:13343#0/2_CONS_SUB_SUB_CMP merged_sample={'26a_F040644': 1}; seq_rank=55; count=1; seq_length=99; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactacgagcaaca +gcctgaaactcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:30:1800:7833#0/2_CONS_SUB_SUB merged_sample={'15a_F730814': 1}; seq_rank=56; count=1; seq_length=100; +ttagccctaaacacaagacattaatataacgagattaatcgacagagtactaccggctat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:108:9222:18258#0/2_CONS_SUB_SUB merged_sample={'13a_F730603': 1}; seq_rank=57; count=1; seq_length=100; +ctagccttaaacacaaatagttatgcagacaaaactattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:38:3005:20881#0/2_CONS_SUB_SUB_CMP merged_sample={'26a_F040644': 1}; seq_rank=58; count=1; seq_length=99; +ttagccctaaacatgaacattcaataaacaagaatgttcgccagagtactactagcaaca +gcctgaaactcaaaggacttggcggtgctttacatccct diff -r 000000000000 -r aca6a8c9cf83 test-data/output_obiclean_advanced.fasta --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/output_obiclean_advanced.fasta Wed Apr 12 17:38:00 2017 -0400 @@ -0,0 +1,172 @@ +>HELIUM_000100422_612GNAAXX:7:119:14871:19157#0/2_CONS_SUB_SUB_CMP merged_sample={'29a_F260619': 1}; obiclean_internalcount=0; obiclean_singletoncount=1; count=1; seq_length=100; obiclean_headcount=0; seq_rank=1; obiclean_samplecount=1; obiclean_head=True; +ttagccctaaacacaagtaattattataacaaaatcattcgccagagtactaccggcaat +agctcaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:108:5640:3823#0/2_CONS_SUB_SUB_CMP merged_sample={'29a_F260619': 1}; obiclean_internalcount=1; obiclean_singletoncount=0; count=1; seq_length=100; obiclean_headcount=0; seq_rank=2; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacacaagtaattaatataacaaaattgttcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:97:14311:19299#0/2_CONS_SUB_SUB_CMP merged_sample={'29a_F260619': 1}; obiclean_internalcount=1; obiclean_singletoncount=0; count=1; seq_length=100; obiclean_headcount=0; seq_rank=3; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaag +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:22:8540:14708#0/2_CONS_SUB_SUB merged_sample={'29a_F260619': 30, '15a_F730814': 31}; obiclean_internalcount=0; obiclean_singletoncount=0; count=61; seq_length=100; obiclean_headcount=2; seq_rank=4; obiclean_samplecount=2; obiclean_head=True; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:57:18459:16145#0/2_CONS_SUB_SUB_CMP merged_sample={'26a_F040644': 64}; obiclean_internalcount=0; obiclean_singletoncount=0; count=64; seq_length=99; obiclean_headcount=1; seq_rank=5; obiclean_samplecount=1; obiclean_head=True; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca +gcctgaaactcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:89:10281:5930#0/2_CONS_SUB_SUB_CMP merged_sample={'29a_F260619': 3}; obiclean_internalcount=0; obiclean_singletoncount=1; count=3; seq_length=100; obiclean_headcount=0; seq_rank=6; obiclean_samplecount=1; obiclean_head=True; +ttagccctaaacacaaataattacacaaacaaaattgttcaccagagtactagcggcaac +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:29:15520:18035#0/2_CONS_SUB_SUB merged_sample={'29a_F260619': 2}; obiclean_internalcount=0; obiclean_singletoncount=1; count=2; seq_length=100; obiclean_headcount=0; seq_rank=7; obiclean_samplecount=1; obiclean_head=True; +ttagccctaaacacaagtaattattataacaaaattattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:6:9274:14951#0/2_CONS_SUB_SUB merged_sample={'13a_F730603': 46}; obiclean_internalcount=0; obiclean_singletoncount=0; count=46; seq_length=100; obiclean_headcount=1; seq_rank=8; obiclean_samplecount=1; obiclean_head=True; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:24:16230:12703#0/2_CONS_SUB_SUB_CMP merged_sample={'15a_F730814': 1}; obiclean_internalcount=1; obiclean_singletoncount=0; count=1; seq_length=100; obiclean_headcount=0; seq_rank=9; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttatcccctt +>HELIUM_000100422_612GNAAXX:7:4:4214:9434#0/2_CONS_SUB_SUB merged_sample={'13a_F730603': 1}; obiclean_internalcount=1; obiclean_singletoncount=0; count=1; seq_length=100; obiclean_headcount=0; seq_rank=10; obiclean_samplecount=1; obiclean_head=False; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactacaggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:13:6954:13039#0/2_CONS_SUB_SUB_CMP merged_sample={'26a_F040644': 1}; obiclean_internalcount=0; obiclean_singletoncount=1; count=1; seq_length=99; obiclean_headcount=0; seq_rank=11; obiclean_samplecount=1; obiclean_head=True; +ttagccctaaacataaacattcaataaacaagaatgttcgccagaggactactagcaata +gcttaaaactcaaaggacttggcggtgctttatatccct +>HELIUM_000100422_612GNAAXX:7:38:6201:12003#0/2_CONS_SUB_SUB_CMP merged_sample={'26a_F040644': 1}; obiclean_internalcount=0; obiclean_singletoncount=1; count=1; seq_length=99; obiclean_headcount=0; seq_rank=12; obiclean_samplecount=1; obiclean_head=True; +ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaata +gcttaaaactcaaagaactgggcggtgctttatatcccg +>HELIUM_000100422_612GNAAXX:7:115:17402:4174#0/2_CONS_SUB_SUB_CMP merged_sample={'29a_F260619': 1, '15a_F730814': 1}; obiclean_internalcount=2; obiclean_singletoncount=0; count=2; seq_length=100; obiclean_headcount=0; seq_rank=13; obiclean_samplecount=2; obiclean_head=False; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactacctgcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:78:7151:19928#0/2_CONS_SUB_SUB merged_sample={'26a_F040644': 1}; obiclean_internalcount=0; obiclean_singletoncount=1; count=1; seq_length=99; obiclean_headcount=0; seq_rank=14; obiclean_samplecount=1; obiclean_head=True; +ttagccctaaacataaacattcaataaacgagaatgttcgccagtgtacttctagcaaca +ggctgaaactcaaaggacttggcggtggtttacatccct +>HELIUM_000100422_612GNAAXX:7:38:10813:16867#0/2_CONS_SUB_SUB merged_sample={'26a_F040644': 2}; obiclean_internalcount=1; obiclean_singletoncount=0; count=2; seq_length=99; obiclean_headcount=0; seq_rank=15; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacataaacattcaataaacaagaatgtttgccagagtactactagcaaca +gcctgaaactcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:13:5742:9859#0/2_CONS_SUB_SUB merged_sample={'29a_F260619': 7}; obiclean_internalcount=0; obiclean_singletoncount=1; count=7; seq_length=4; obiclean_headcount=0; seq_rank=16; obiclean_samplecount=1; obiclean_head=True; +tttt +>HELIUM_000100422_612GNAAXX:7:16:16357:19948#0/2_CONS_SUB_SUB merged_sample={'26a_F040644': 1}; obiclean_internalcount=1; obiclean_singletoncount=0; count=1; seq_length=99; obiclean_headcount=0; seq_rank=17; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca +gcctgaaactcacaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:107:3570:18147#0/2_CONS_SUB_SUB_CMP merged_sample={'29a_F260619': 1}; obiclean_internalcount=1; obiclean_singletoncount=0; count=1; seq_length=100; obiclean_headcount=0; seq_rank=18; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat +agcataaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:114:4511:17596#0/2_CONS_SUB_SUB_CMP merged_sample={'29a_F260619': 1}; obiclean_internalcount=0; obiclean_singletoncount=1; count=1; seq_length=100; obiclean_headcount=0; seq_rank=19; obiclean_samplecount=1; obiclean_head=True; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtgctaccggcaat +agcttaaaactcaaaggacttggcggtgctttatacgctt +>HELIUM_000100422_612GNAAXX:7:70:11798:2668#0/2_CONS_SUB_SUB merged_sample={'15a_F730814': 2}; obiclean_internalcount=0; obiclean_singletoncount=1; count=2; seq_length=100; obiclean_headcount=0; seq_rank=20; obiclean_samplecount=1; obiclean_head=True; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccttt +>HELIUM_000100422_612GNAAXX:7:29:18416:16620#0/2_CONS_SUB_SUB_CMP merged_sample={'29a_F260619': 1}; obiclean_internalcount=0; obiclean_singletoncount=1; count=1; seq_length=100; obiclean_headcount=0; seq_rank=21; obiclean_samplecount=1; obiclean_head=True; +ttagccctaaacacaagtaattattataacaaaattattcgccagagttctaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctg +>HELIUM_000100422_612GNAAXX:7:120:1900:13295#0/2_CONS_SUB_SUB_CMP merged_sample={'15a_F730814': 1}; obiclean_internalcount=0; obiclean_singletoncount=1; count=1; seq_length=100; obiclean_headcount=0; seq_rank=22; obiclean_samplecount=1; obiclean_head=True; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactacgcgcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:76:10822:18722#0/2_CONS_SUB_SUB merged_sample={'26a_F040644': 1}; obiclean_internalcount=1; obiclean_singletoncount=0; count=1; seq_length=99; obiclean_headcount=0; seq_rank=23; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacataaacattcaataaacacgaatgttcgccagagtactactagcaaca +gcctgaaactcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:76:8491:8900#0/2_CONS_SUB_SUB_CMP merged_sample={'15a_F730814': 1}; obiclean_internalcount=0; obiclean_singletoncount=1; count=1; seq_length=100; obiclean_headcount=0; seq_rank=24; obiclean_samplecount=1; obiclean_head=True; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtgctacctgcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:9:10358:4089#0/2_CONS_SUB_SUB_CMP merged_sample={'13a_F730603': 1}; obiclean_internalcount=0; obiclean_singletoncount=1; count=1; seq_length=100; obiclean_headcount=0; seq_rank=25; obiclean_samplecount=1; obiclean_head=True; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactacaggcaat +agctcaaaactcaaagaacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:59:11971:12063#0/2_CONS_SUB_SUB merged_sample={'29a_F260619': 1}; obiclean_internalcount=1; obiclean_singletoncount=0; count=1; seq_length=100; obiclean_headcount=0; seq_rank=26; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacacaagtaattaatataacaaaattattcgtcagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:111:19168:18517#0/2_CONS_SUB_SUB merged_sample={'15a_F730814': 1}; obiclean_internalcount=1; obiclean_singletoncount=0; count=1; seq_length=100; obiclean_headcount=0; seq_rank=27; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacacaagtaattaatataacaaaattatccgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:41:9316:2256#0/2_CONS_SUB_SUB merged_sample={'29a_F260619': 1}; obiclean_internalcount=1; obiclean_singletoncount=0; count=1; seq_length=100; obiclean_headcount=0; seq_rank=28; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacacaagaaattaatataacaaaattattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:33:9900:9541#0/2_CONS_SUB_SUB merged_sample={'13a_F730603': 1}; obiclean_internalcount=1; obiclean_singletoncount=0; count=1; seq_length=100; obiclean_headcount=0; seq_rank=29; obiclean_samplecount=1; obiclean_head=False; +ctagccctaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:95:1688:9598#0/2_CONS_SUB_SUB_CMP merged_sample={'26a_F040644': 1}; obiclean_internalcount=0; obiclean_singletoncount=1; count=1; seq_length=99; obiclean_headcount=0; seq_rank=30; obiclean_samplecount=1; obiclean_head=True; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtgatactagcaaca +gcctgaaactcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:55:16528:11418#0/2_CONS_SUB_SUB merged_sample={'13a_F730603': 1}; obiclean_internalcount=0; obiclean_singletoncount=1; count=1; seq_length=100; obiclean_headcount=0; seq_rank=31; obiclean_samplecount=1; obiclean_head=True; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcact +agcttacaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:19:6313:16567#0/2_CONS_SUB_SUB_CMP merged_sample={'26a_F040644': 1}; obiclean_internalcount=1; obiclean_singletoncount=0; count=1; seq_length=99; obiclean_headcount=0; seq_rank=32; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca +gcctgaaactcaaagggcttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:111:8478:1127#0/2_CONS_SUB_SUB_CMP merged_sample={'26a_F040644': 1}; obiclean_internalcount=0; obiclean_singletoncount=1; count=1; seq_length=99; obiclean_headcount=0; seq_rank=33; obiclean_samplecount=1; obiclean_head=True; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca +gcctggaacgcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:81:15726:2345#0/2_CONS_SUB_SUB_CMP merged_sample={'26a_F040644': 1}; obiclean_internalcount=0; obiclean_singletoncount=1; count=1; seq_length=99; obiclean_headcount=0; seq_rank=34; obiclean_samplecount=1; obiclean_head=True; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca +gcctgaaactcaaagcactcggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:7:11110:17924#0/2_CONS_SUB_SUB merged_sample={'15a_F730814': 1}; obiclean_internalcount=0; obiclean_singletoncount=1; count=1; seq_length=100; obiclean_headcount=0; seq_rank=35; obiclean_samplecount=1; obiclean_head=True; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtcataccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:89:9710:20652#0/2_CONS_SUB_SUB merged_sample={'26a_F040644': 1}; obiclean_internalcount=1; obiclean_singletoncount=0; count=1; seq_length=99; obiclean_headcount=0; seq_rank=36; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca +gcctgaaactcaaaggacttggcggtgctttatatccct +>HELIUM_000100422_612GNAAXX:7:71:17473:7401#0/2_CONS_SUB_SUB_CMP merged_sample={'15a_F730814': 1}; obiclean_internalcount=1; obiclean_singletoncount=0; count=1; seq_length=100; obiclean_headcount=0; seq_rank=37; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggtaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:115:3625:10051#0/2_CONS_SUB_SUB merged_sample={'13a_F730603': 1}; obiclean_internalcount=0; obiclean_singletoncount=1; count=1; seq_length=5; obiclean_headcount=0; seq_rank=38; obiclean_samplecount=1; obiclean_head=True; +caata +>HELIUM_000100422_612GNAAXX:7:99:8183:13912#0/2_CONS_SUB_SUB merged_sample={'13a_F730603': 1}; obiclean_internalcount=1; obiclean_singletoncount=0; count=1; seq_length=100; obiclean_headcount=0; seq_rank=39; obiclean_samplecount=1; obiclean_head=False; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctc +>HELIUM_000100422_612GNAAXX:7:22:1798:3790#0/2_CONS_SUB_SUB_CMP merged_sample={'13a_F730603': 1}; obiclean_internalcount=1; obiclean_singletoncount=0; count=1; seq_length=100; obiclean_headcount=0; seq_rank=40; obiclean_samplecount=1; obiclean_head=False; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtgctaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:115:14354:10118#0/2_CONS_SUB_SUB_CMP merged_sample={'26a_F040644': 1}; obiclean_internalcount=1; obiclean_singletoncount=0; count=1; seq_length=99; obiclean_headcount=0; seq_rank=41; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactcgcaaca +gcctgaaactcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:103:3594:15996#0/2_CONS_SUB_SUB merged_sample={'13a_F730603': 1}; obiclean_internalcount=1; obiclean_singletoncount=0; count=1; seq_length=100; obiclean_headcount=0; seq_rank=42; obiclean_samplecount=1; obiclean_head=False; +ctagccttaaacacaaatagttatgcaaacacaactattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:105:14135:6905#0/2_CONS_SUB_SUB merged_sample={'29a_F260619': 1}; obiclean_internalcount=0; obiclean_singletoncount=1; count=1; seq_length=100; obiclean_headcount=0; seq_rank=43; obiclean_samplecount=1; obiclean_head=True; +ttagccctaaacacaagtaattaatataacaaaattattcaccagagtactagcggcaac +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:76:18268:14980#0/2_CONS_SUB_SUB_CMP merged_sample={'29a_F260619': 1}; obiclean_internalcount=1; obiclean_singletoncount=0; count=1; seq_length=100; obiclean_headcount=0; seq_rank=44; obiclean_samplecount=1; obiclean_head=False; +ttacccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:25:7789:3090#0/2_CONS_SUB_SUB_CMP merged_sample={'29a_F260619': 1}; obiclean_internalcount=1; obiclean_singletoncount=0; count=1; seq_length=100; obiclean_headcount=0; seq_rank=45; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactacaggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:42:19757:3800#0/2_CONS_SUB_SUB_CMP merged_sample={'15a_F730814': 1}; obiclean_internalcount=1; obiclean_singletoncount=0; count=1; seq_length=99; obiclean_headcount=0; seq_rank=46; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacacaagtaattaatatacaaaattattcgccagagtactaccggcaata +gcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:116:14244:17926#0/2_CONS_SUB_SUB merged_sample={'26a_F040644': 1}; obiclean_internalcount=1; obiclean_singletoncount=0; count=1; seq_length=99; obiclean_headcount=0; seq_rank=47; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacataaacattcaataaacaagaatgttcgcaagagtactactagcaaca +gcctgaaactcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:72:12959:5736#0/2_CONS_SUB_SUB_CMP merged_sample={'26a_F040644': 1}; obiclean_internalcount=1; obiclean_singletoncount=0; count=1; seq_length=99; obiclean_headcount=0; seq_rank=48; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca +gcctgaaactcaaaggacttggcggtgctttacacccct +>HELIUM_000100422_612GNAAXX:7:7:15538:8325#0/2_CONS_SUB_SUB_CMP merged_sample={'26a_F040644': 1}; obiclean_internalcount=0; obiclean_singletoncount=1; count=1; seq_length=99; obiclean_headcount=0; seq_rank=49; obiclean_samplecount=1; obiclean_head=True; +ttagccctaaacataaacattcaataaacaagaatgtcggccagagtactactagcaaca +gcatgaaactcaaagaactgggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:120:16005:7955#0/2_CONS_SUB_SUB_CMP merged_sample={'26a_F040644': 1}; obiclean_internalcount=1; obiclean_singletoncount=0; count=1; seq_length=98; obiclean_headcount=0; seq_rank=50; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca +gccgaaactcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:10:9237:10532#0/2_CONS_SUB_SUB_CMP merged_sample={'29a_F260619': 1}; obiclean_internalcount=1; obiclean_singletoncount=0; count=1; seq_length=100; obiclean_headcount=0; seq_rank=51; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacacaagtaattaatataacaaaataattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:7:11998:4462#0/2_CONS_SUB_SUB merged_sample={'26a_F040644': 1}; obiclean_internalcount=1; obiclean_singletoncount=0; count=1; seq_length=99; obiclean_headcount=0; seq_rank=52; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtagtactagcaaca +gcctgaaactcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:111:18277:17779#0/2_CONS_SUB_SUB_CMP merged_sample={'26a_F040644': 1}; obiclean_internalcount=0; obiclean_singletoncount=1; count=1; seq_length=99; obiclean_headcount=0; seq_rank=53; obiclean_samplecount=1; obiclean_head=True; +ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaata +gcctgaaactcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:3:17077:6562#0/2_CONS_SUB_SUB_CMP merged_sample={'15a_F730814': 1}; obiclean_internalcount=1; obiclean_singletoncount=0; count=1; seq_length=100; obiclean_headcount=0; seq_rank=54; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactgccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:19:4311:13343#0/2_CONS_SUB_SUB_CMP merged_sample={'26a_F040644': 1}; obiclean_internalcount=1; obiclean_singletoncount=0; count=1; seq_length=99; obiclean_headcount=0; seq_rank=55; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactacgagcaaca +gcctgaaactcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:30:1800:7833#0/2_CONS_SUB_SUB merged_sample={'15a_F730814': 1}; obiclean_internalcount=0; obiclean_singletoncount=1; count=1; seq_length=100; obiclean_headcount=0; seq_rank=56; obiclean_samplecount=1; obiclean_head=True; +ttagccctaaacacaagacattaatataacgagattaatcgacagagtactaccggctat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:108:9222:18258#0/2_CONS_SUB_SUB merged_sample={'13a_F730603': 1}; obiclean_internalcount=1; obiclean_singletoncount=0; count=1; seq_length=100; obiclean_headcount=0; seq_rank=57; obiclean_samplecount=1; obiclean_head=False; +ctagccttaaacacaaatagttatgcagacaaaactattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:38:3005:20881#0/2_CONS_SUB_SUB_CMP merged_sample={'26a_F040644': 1}; obiclean_internalcount=1; obiclean_singletoncount=0; count=1; seq_length=99; obiclean_headcount=0; seq_rank=58; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacatgaacattcaataaacaagaatgttcgccagagtactactagcaaca +gcctgaaactcaaaggacttggcggtgctttacatccct diff -r 000000000000 -r aca6a8c9cf83 test-data/output_obiclean_simple.fasta --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/output_obiclean_simple.fasta Wed Apr 12 17:38:00 2017 -0400 @@ -0,0 +1,67 @@ +>HELIUM_000100422_612GNAAXX:7:119:14871:19157#0/2_CONS_SUB_SUB_CMP count=1; obiclean_count={'XXX': 1}; obiclean_head=True; obiclean_cluster={'XXX': 'HELIUM_000100422_612GNAAXX:7:119:14871:19157#0/2_CONS_SUB_SUB_CMP'}; seq_length=100; obiclean_internalcount=0; obiclean_status={'XXX': 's'}; seq_rank=1; merged_sample={'29a_F260619': 1}; obiclean_samplecount=1; obiclean_headcount=0; obiclean_singletoncount=1; +ttagccctaaacacaagtaattattataacaaaatcattcgccagagtactaccggcaat +agctcaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:22:8540:14708#0/2_CONS_SUB_SUB count=61; obiclean_count={'XXX': 81}; obiclean_head=True; obiclean_cluster={'XXX': 'HELIUM_000100422_612GNAAXX:7:22:8540:14708#0/2_CONS_SUB_SUB'}; seq_length=100; obiclean_internalcount=0; obiclean_status={'XXX': 'h'}; seq_rank=4; merged_sample={'29a_F260619': 30, '15a_F730814': 31}; obiclean_samplecount=1; obiclean_headcount=1; obiclean_singletoncount=0; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:57:18459:16145#0/2_CONS_SUB_SUB_CMP count=64; obiclean_count={'XXX': 77}; obiclean_head=True; obiclean_cluster={'XXX': 'HELIUM_000100422_612GNAAXX:7:57:18459:16145#0/2_CONS_SUB_SUB_CMP'}; seq_length=99; obiclean_internalcount=0; obiclean_status={'XXX': 'h'}; seq_rank=5; merged_sample={'26a_F040644': 64}; obiclean_samplecount=1; obiclean_headcount=1; obiclean_singletoncount=0; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca +gcctgaaactcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:89:10281:5930#0/2_CONS_SUB_SUB_CMP count=3; obiclean_count={'XXX': 3}; obiclean_head=True; obiclean_cluster={'XXX': 'HELIUM_000100422_612GNAAXX:7:89:10281:5930#0/2_CONS_SUB_SUB_CMP'}; seq_length=100; obiclean_internalcount=0; obiclean_status={'XXX': 's'}; seq_rank=6; merged_sample={'29a_F260619': 3}; obiclean_samplecount=1; obiclean_headcount=0; obiclean_singletoncount=1; +ttagccctaaacacaaataattacacaaacaaaattgttcaccagagtactagcggcaac +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:6:9274:14951#0/2_CONS_SUB_SUB count=46; obiclean_count={'XXX': 52}; obiclean_head=True; obiclean_cluster={'XXX': 'HELIUM_000100422_612GNAAXX:7:6:9274:14951#0/2_CONS_SUB_SUB'}; seq_length=100; obiclean_internalcount=0; obiclean_status={'XXX': 'h'}; seq_rank=8; merged_sample={'13a_F730603': 46}; obiclean_samplecount=1; obiclean_headcount=1; obiclean_singletoncount=0; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:13:6954:13039#0/2_CONS_SUB_SUB_CMP count=1; obiclean_count={'XXX': 1}; obiclean_head=True; obiclean_cluster={'XXX': 'HELIUM_000100422_612GNAAXX:7:13:6954:13039#0/2_CONS_SUB_SUB_CMP'}; seq_length=99; obiclean_internalcount=0; obiclean_status={'XXX': 's'}; seq_rank=11; merged_sample={'26a_F040644': 1}; obiclean_samplecount=1; obiclean_headcount=0; obiclean_singletoncount=1; +ttagccctaaacataaacattcaataaacaagaatgttcgccagaggactactagcaata +gcttaaaactcaaaggacttggcggtgctttatatccct +>HELIUM_000100422_612GNAAXX:7:38:6201:12003#0/2_CONS_SUB_SUB_CMP count=1; obiclean_count={'XXX': 1}; obiclean_head=True; obiclean_cluster={'XXX': 'HELIUM_000100422_612GNAAXX:7:38:6201:12003#0/2_CONS_SUB_SUB_CMP'}; seq_length=99; obiclean_internalcount=0; obiclean_status={'XXX': 's'}; seq_rank=12; merged_sample={'26a_F040644': 1}; obiclean_samplecount=1; obiclean_headcount=0; obiclean_singletoncount=1; +ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaata +gcttaaaactcaaagaactgggcggtgctttatatcccg +>HELIUM_000100422_612GNAAXX:7:78:7151:19928#0/2_CONS_SUB_SUB count=1; obiclean_count={'XXX': 1}; obiclean_head=True; obiclean_cluster={'XXX': 'HELIUM_000100422_612GNAAXX:7:78:7151:19928#0/2_CONS_SUB_SUB'}; seq_length=99; obiclean_internalcount=0; obiclean_status={'XXX': 's'}; seq_rank=14; merged_sample={'26a_F040644': 1}; obiclean_samplecount=1; obiclean_headcount=0; obiclean_singletoncount=1; +ttagccctaaacataaacattcaataaacgagaatgttcgccagtgtacttctagcaaca +ggctgaaactcaaaggacttggcggtggtttacatccct +>HELIUM_000100422_612GNAAXX:7:13:5742:9859#0/2_CONS_SUB_SUB count=7; obiclean_count={'XXX': 7}; obiclean_head=True; obiclean_cluster={'XXX': 'HELIUM_000100422_612GNAAXX:7:13:5742:9859#0/2_CONS_SUB_SUB'}; seq_length=4; obiclean_internalcount=0; obiclean_status={'XXX': 's'}; seq_rank=16; merged_sample={'29a_F260619': 7}; obiclean_samplecount=1; obiclean_headcount=0; obiclean_singletoncount=1; +tttt +>HELIUM_000100422_612GNAAXX:7:114:4511:17596#0/2_CONS_SUB_SUB_CMP count=1; obiclean_count={'XXX': 1}; obiclean_head=True; obiclean_cluster={'XXX': 'HELIUM_000100422_612GNAAXX:7:114:4511:17596#0/2_CONS_SUB_SUB_CMP'}; seq_length=100; obiclean_internalcount=0; obiclean_status={'XXX': 's'}; seq_rank=19; merged_sample={'29a_F260619': 1}; obiclean_samplecount=1; obiclean_headcount=0; obiclean_singletoncount=1; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtgctaccggcaat +agcttaaaactcaaaggacttggcggtgctttatacgctt +>HELIUM_000100422_612GNAAXX:7:29:18416:16620#0/2_CONS_SUB_SUB_CMP count=1; obiclean_count={'XXX': 1}; obiclean_head=True; obiclean_cluster={'XXX': 'HELIUM_000100422_612GNAAXX:7:29:18416:16620#0/2_CONS_SUB_SUB_CMP'}; seq_length=100; obiclean_internalcount=0; obiclean_status={'XXX': 's'}; seq_rank=21; merged_sample={'29a_F260619': 1}; obiclean_samplecount=1; obiclean_headcount=0; obiclean_singletoncount=1; +ttagccctaaacacaagtaattattataacaaaattattcgccagagttctaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctg +>HELIUM_000100422_612GNAAXX:7:120:1900:13295#0/2_CONS_SUB_SUB_CMP count=1; obiclean_count={'XXX': 1}; obiclean_head=True; obiclean_cluster={'XXX': 'HELIUM_000100422_612GNAAXX:7:120:1900:13295#0/2_CONS_SUB_SUB_CMP'}; seq_length=100; obiclean_internalcount=0; obiclean_status={'XXX': 's'}; seq_rank=22; merged_sample={'15a_F730814': 1}; obiclean_samplecount=1; obiclean_headcount=0; obiclean_singletoncount=1; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactacgcgcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:9:10358:4089#0/2_CONS_SUB_SUB_CMP count=1; obiclean_count={'XXX': 1}; obiclean_head=True; obiclean_cluster={'XXX': 'HELIUM_000100422_612GNAAXX:7:9:10358:4089#0/2_CONS_SUB_SUB_CMP'}; seq_length=100; obiclean_internalcount=0; obiclean_status={'XXX': 's'}; seq_rank=25; merged_sample={'13a_F730603': 1}; obiclean_samplecount=1; obiclean_headcount=0; obiclean_singletoncount=1; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactacaggcaat +agctcaaaactcaaagaacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:95:1688:9598#0/2_CONS_SUB_SUB_CMP count=1; obiclean_count={'XXX': 1}; obiclean_head=True; obiclean_cluster={'XXX': 'HELIUM_000100422_612GNAAXX:7:95:1688:9598#0/2_CONS_SUB_SUB_CMP'}; seq_length=99; obiclean_internalcount=0; obiclean_status={'XXX': 's'}; seq_rank=30; merged_sample={'26a_F040644': 1}; obiclean_samplecount=1; obiclean_headcount=0; obiclean_singletoncount=1; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtgatactagcaaca +gcctgaaactcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:55:16528:11418#0/2_CONS_SUB_SUB count=1; obiclean_count={'XXX': 1}; obiclean_head=True; obiclean_cluster={'XXX': 'HELIUM_000100422_612GNAAXX:7:55:16528:11418#0/2_CONS_SUB_SUB'}; seq_length=100; obiclean_internalcount=0; obiclean_status={'XXX': 's'}; seq_rank=31; merged_sample={'13a_F730603': 1}; obiclean_samplecount=1; obiclean_headcount=0; obiclean_singletoncount=1; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcact +agcttacaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:111:8478:1127#0/2_CONS_SUB_SUB_CMP count=1; obiclean_count={'XXX': 1}; obiclean_head=True; obiclean_cluster={'XXX': 'HELIUM_000100422_612GNAAXX:7:111:8478:1127#0/2_CONS_SUB_SUB_CMP'}; seq_length=99; obiclean_internalcount=0; obiclean_status={'XXX': 's'}; seq_rank=33; merged_sample={'26a_F040644': 1}; obiclean_samplecount=1; obiclean_headcount=0; obiclean_singletoncount=1; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca +gcctggaacgcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:81:15726:2345#0/2_CONS_SUB_SUB_CMP count=1; obiclean_count={'XXX': 1}; obiclean_head=True; obiclean_cluster={'XXX': 'HELIUM_000100422_612GNAAXX:7:81:15726:2345#0/2_CONS_SUB_SUB_CMP'}; seq_length=99; obiclean_internalcount=0; obiclean_status={'XXX': 's'}; seq_rank=34; merged_sample={'26a_F040644': 1}; obiclean_samplecount=1; obiclean_headcount=0; obiclean_singletoncount=1; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca +gcctgaaactcaaagcactcggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:7:11110:17924#0/2_CONS_SUB_SUB count=1; obiclean_count={'XXX': 1}; obiclean_head=True; obiclean_cluster={'XXX': 'HELIUM_000100422_612GNAAXX:7:7:11110:17924#0/2_CONS_SUB_SUB'}; seq_length=100; obiclean_internalcount=0; obiclean_status={'XXX': 's'}; seq_rank=35; merged_sample={'15a_F730814': 1}; obiclean_samplecount=1; obiclean_headcount=0; obiclean_singletoncount=1; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtcataccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:115:3625:10051#0/2_CONS_SUB_SUB count=1; obiclean_count={'XXX': 1}; obiclean_head=True; obiclean_cluster={'XXX': 'HELIUM_000100422_612GNAAXX:7:115:3625:10051#0/2_CONS_SUB_SUB'}; seq_length=5; obiclean_internalcount=0; obiclean_status={'XXX': 's'}; seq_rank=38; merged_sample={'13a_F730603': 1}; obiclean_samplecount=1; obiclean_headcount=0; obiclean_singletoncount=1; +caata +>HELIUM_000100422_612GNAAXX:7:105:14135:6905#0/2_CONS_SUB_SUB count=1; obiclean_count={'XXX': 1}; obiclean_head=True; obiclean_cluster={'XXX': 'HELIUM_000100422_612GNAAXX:7:105:14135:6905#0/2_CONS_SUB_SUB'}; seq_length=100; obiclean_internalcount=0; obiclean_status={'XXX': 's'}; seq_rank=43; merged_sample={'29a_F260619': 1}; obiclean_samplecount=1; obiclean_headcount=0; obiclean_singletoncount=1; +ttagccctaaacacaagtaattaatataacaaaattattcaccagagtactagcggcaac +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:7:15538:8325#0/2_CONS_SUB_SUB_CMP count=1; obiclean_count={'XXX': 1}; obiclean_head=True; obiclean_cluster={'XXX': 'HELIUM_000100422_612GNAAXX:7:7:15538:8325#0/2_CONS_SUB_SUB_CMP'}; seq_length=99; obiclean_internalcount=0; obiclean_status={'XXX': 's'}; seq_rank=49; merged_sample={'26a_F040644': 1}; obiclean_samplecount=1; obiclean_headcount=0; obiclean_singletoncount=1; +ttagccctaaacataaacattcaataaacaagaatgtcggccagagtactactagcaaca +gcatgaaactcaaagaactgggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:111:18277:17779#0/2_CONS_SUB_SUB_CMP count=1; obiclean_count={'XXX': 1}; obiclean_head=True; obiclean_cluster={'XXX': 'HELIUM_000100422_612GNAAXX:7:111:18277:17779#0/2_CONS_SUB_SUB_CMP'}; seq_length=99; obiclean_internalcount=0; obiclean_status={'XXX': 's'}; seq_rank=53; merged_sample={'26a_F040644': 1}; obiclean_samplecount=1; obiclean_headcount=0; obiclean_singletoncount=1; +ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaata +gcctgaaactcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:30:1800:7833#0/2_CONS_SUB_SUB count=1; obiclean_count={'XXX': 1}; obiclean_head=True; obiclean_cluster={'XXX': 'HELIUM_000100422_612GNAAXX:7:30:1800:7833#0/2_CONS_SUB_SUB'}; seq_length=100; obiclean_internalcount=0; obiclean_status={'XXX': 's'}; seq_rank=56; merged_sample={'15a_F730814': 1}; obiclean_samplecount=1; obiclean_headcount=0; obiclean_singletoncount=1; +ttagccctaaacacaagacattaatataacgagattaatcgacagagtactaccggctat +agcttaaaactcaaaggacttggcggtgctttataccctt diff -r 000000000000 -r aca6a8c9cf83 test-data/output_obiconvert.fasta --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/output_obiconvert.fasta Wed Apr 12 17:38:00 2017 -0400 @@ -0,0 +1,172 @@ +>HELIUM_000100422_612GNAAXX:7:119:14871:19157#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'29a_F260619': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=1; obiclean_samplecount=1; obiclean_head=True; +TTAGCCCTAAACACAAGTAATTATTATAACAAAATCATTCGCCAGAGTACTACCGGCAAT +AGCTCAAAACTCAAAGGACTTGGCGGTGCTTTATACCCTT +>HELIUM_000100422_612GNAAXX:7:108:5640:3823#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'29a_F260619': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=2; obiclean_samplecount=1; obiclean_head=False; +TTAGCCCTAAACACAAGTAATTAATATAACAAAATTGTTCGCCAGAGTACTACCGGCAAT +AGCTTAAAACTCAAAGGACTTGGCGGTGCTTTATACCCTT +>HELIUM_000100422_612GNAAXX:7:97:14311:19299#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'29a_F260619': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=3; obiclean_samplecount=1; obiclean_head=False; +TTAGCCCTAAACACAAGTAATTAATATAACAAAATTATTCGCCAGAGTACTACCGGCAAG +AGCTTAAAACTCAAAGGACTTGGCGGTGCTTTATACCCTT +>HELIUM_000100422_612GNAAXX:7:24:16230:12703#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'15a_F730814': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=9; obiclean_samplecount=1; obiclean_head=False; +TTAGCCCTAAACACAAGTAATTAATATAACAAAATTATTCGCCAGAGTACTACCGGCAAT +AGCTTAAAACTCAAAGGACTTGGCGGTGCTTTATCCCCTT +>HELIUM_000100422_612GNAAXX:7:4:4214:9434#0/2_CONS_SUB_SUB count=1; merged_sample={'13a_F730603': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=10; obiclean_samplecount=1; obiclean_head=False; +CTAGCCTTAAACACAAATAGTTATGCAAACAAAACTATTCGCCAGAGTACTACAGGCAAT +AGCTTAAAACTCAAAGGACTTGGCGGTGCTTTATACCCTT +>HELIUM_000100422_612GNAAXX:7:13:6954:13039#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=99; obiclean_headcount=0; seq_rank=11; obiclean_samplecount=1; obiclean_head=True; +TTAGCCCTAAACATAAACATTCAATAAACAAGAATGTTCGCCAGAGGACTACTAGCAATA +GCTTAAAACTCAAAGGACTTGGCGGTGCTTTATATCCCT +>HELIUM_000100422_612GNAAXX:7:38:6201:12003#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=99; obiclean_headcount=0; seq_rank=12; obiclean_samplecount=1; obiclean_head=True; +TTAGCCCTAAACATAGATAATTTTACAACAAAATAATTCGCCAGAGGACTACTAGCAATA +GCTTAAAACTCAAAGAACTGGGCGGTGCTTTATATCCCG +>HELIUM_000100422_612GNAAXX:7:78:7151:19928#0/2_CONS_SUB_SUB count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=99; obiclean_headcount=0; seq_rank=14; obiclean_samplecount=1; obiclean_head=True; +TTAGCCCTAAACATAAACATTCAATAAACGAGAATGTTCGCCAGTGTACTTCTAGCAACA +GGCTGAAACTCAAAGGACTTGGCGGTGGTTTACATCCCT +>HELIUM_000100422_612GNAAXX:7:16:16357:19948#0/2_CONS_SUB_SUB count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=99; obiclean_headcount=0; seq_rank=17; obiclean_samplecount=1; obiclean_head=False; +TTAGCCCTAAACATAAACATTCAATAAACAAGAATGTTCGCCAGAGTACTACTAGCAACA +GCCTGAAACTCACAGGACTTGGCGGTGCTTTACATCCCT +>HELIUM_000100422_612GNAAXX:7:107:3570:18147#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'29a_F260619': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=18; obiclean_samplecount=1; obiclean_head=False; +TTAGCCCTAAACACAAGTAATTAATATAACAAAATTATTCGCCAGAGTACTACCGGCAAT +AGCATAAAACTCAAAGGACTTGGCGGTGCTTTATACCCTT +>HELIUM_000100422_612GNAAXX:7:114:4511:17596#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'29a_F260619': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=19; obiclean_samplecount=1; obiclean_head=True; +TTAGCCCTAAACACAAGTAATTAATATAACAAAATTATTCGCCAGAGTGCTACCGGCAAT +AGCTTAAAACTCAAAGGACTTGGCGGTGCTTTATACGCTT +>HELIUM_000100422_612GNAAXX:7:29:18416:16620#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'29a_F260619': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=21; obiclean_samplecount=1; obiclean_head=True; +TTAGCCCTAAACACAAGTAATTATTATAACAAAATTATTCGCCAGAGTTCTACCGGCAAT +AGCTTAAAACTCAAAGGACTTGGCGGTGCTTTATACCCTG +>HELIUM_000100422_612GNAAXX:7:120:1900:13295#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'15a_F730814': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=22; obiclean_samplecount=1; obiclean_head=True; +TTAGCCCTAAACACAAGTAATTAATATAACAAAATTATTCGCCAGAGTACTACGCGCAAT +AGCTTAAAACTCAAAGGACTTGGCGGTGCTTTATACCCTT +>HELIUM_000100422_612GNAAXX:7:76:10822:18722#0/2_CONS_SUB_SUB count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=99; obiclean_headcount=0; seq_rank=23; obiclean_samplecount=1; obiclean_head=False; +TTAGCCCTAAACATAAACATTCAATAAACACGAATGTTCGCCAGAGTACTACTAGCAACA +GCCTGAAACTCAAAGGACTTGGCGGTGCTTTACATCCCT +>HELIUM_000100422_612GNAAXX:7:76:8491:8900#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'15a_F730814': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=24; obiclean_samplecount=1; obiclean_head=True; +TTAGCCCTAAACACAAGTAATTAATATAACAAAATTATTCGCCAGAGTGCTACCTGCAAT +AGCTTAAAACTCAAAGGACTTGGCGGTGCTTTATACCCTT +>HELIUM_000100422_612GNAAXX:7:9:10358:4089#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'13a_F730603': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=25; obiclean_samplecount=1; obiclean_head=True; +CTAGCCTTAAACACAAATAGTTATGCAAACAAAACTATTCGCCAGAGTACTACAGGCAAT +AGCTCAAAACTCAAAGAACTTGGCGGTGCTTTATACCCTT +>HELIUM_000100422_612GNAAXX:7:59:11971:12063#0/2_CONS_SUB_SUB count=1; merged_sample={'29a_F260619': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=26; obiclean_samplecount=1; obiclean_head=False; +TTAGCCCTAAACACAAGTAATTAATATAACAAAATTATTCGTCAGAGTACTACCGGCAAT +AGCTTAAAACTCAAAGGACTTGGCGGTGCTTTATACCCTT +>HELIUM_000100422_612GNAAXX:7:111:19168:18517#0/2_CONS_SUB_SUB count=1; merged_sample={'15a_F730814': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=27; obiclean_samplecount=1; obiclean_head=False; +TTAGCCCTAAACACAAGTAATTAATATAACAAAATTATCCGCCAGAGTACTACCGGCAAT +AGCTTAAAACTCAAAGGACTTGGCGGTGCTTTATACCCTT +>HELIUM_000100422_612GNAAXX:7:41:9316:2256#0/2_CONS_SUB_SUB count=1; merged_sample={'29a_F260619': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=28; obiclean_samplecount=1; obiclean_head=False; +TTAGCCCTAAACACAAGAAATTAATATAACAAAATTATTCGCCAGAGTACTACCGGCAAT +AGCTTAAAACTCAAAGGACTTGGCGGTGCTTTATACCCTT +>HELIUM_000100422_612GNAAXX:7:33:9900:9541#0/2_CONS_SUB_SUB count=1; merged_sample={'13a_F730603': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=29; obiclean_samplecount=1; obiclean_head=False; +CTAGCCCTAAACACAAATAGTTATGCAAACAAAACTATTCGCCAGAGTACTACCGGCAAT +AGCTTAAAACTCAAAGGACTTGGCGGTGCTTTATACCCTT +>HELIUM_000100422_612GNAAXX:7:95:1688:9598#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=99; obiclean_headcount=0; seq_rank=30; obiclean_samplecount=1; obiclean_head=True; +TTAGCCCTAAACATAAACATTCAATAAACAAGAATGTTCGCCAGAGTGATACTAGCAACA +GCCTGAAACTCAAAGGACTTGGCGGTGCTTTACATCCCT +>HELIUM_000100422_612GNAAXX:7:55:16528:11418#0/2_CONS_SUB_SUB count=1; merged_sample={'13a_F730603': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=31; obiclean_samplecount=1; obiclean_head=True; +CTAGCCTTAAACACAAATAGTTATGCAAACAAAACTATTCGCCAGAGTACTACCGGCACT +AGCTTACAACTCAAAGGACTTGGCGGTGCTTTATACCCTT +>HELIUM_000100422_612GNAAXX:7:19:6313:16567#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=99; obiclean_headcount=0; seq_rank=32; obiclean_samplecount=1; obiclean_head=False; +TTAGCCCTAAACATAAACATTCAATAAACAAGAATGTTCGCCAGAGTACTACTAGCAACA +GCCTGAAACTCAAAGGGCTTGGCGGTGCTTTACATCCCT +>HELIUM_000100422_612GNAAXX:7:111:8478:1127#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=99; obiclean_headcount=0; seq_rank=33; obiclean_samplecount=1; obiclean_head=True; +TTAGCCCTAAACATAAACATTCAATAAACAAGAATGTTCGCCAGAGTACTACTAGCAACA +GCCTGGAACGCAAAGGACTTGGCGGTGCTTTACATCCCT +>HELIUM_000100422_612GNAAXX:7:81:15726:2345#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=99; obiclean_headcount=0; seq_rank=34; obiclean_samplecount=1; obiclean_head=True; +TTAGCCCTAAACATAAACATTCAATAAACAAGAATGTTCGCCAGAGTACTACTAGCAACA +GCCTGAAACTCAAAGCACTCGGCGGTGCTTTACATCCCT +>HELIUM_000100422_612GNAAXX:7:7:11110:17924#0/2_CONS_SUB_SUB count=1; merged_sample={'15a_F730814': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=35; obiclean_samplecount=1; obiclean_head=True; +TTAGCCCTAAACACAAGTAATTAATATAACAAAATTATTCGCCAGAGTCATACCGGCAAT +AGCTTAAAACTCAAAGGACTTGGCGGTGCTTTATACCCTT +>HELIUM_000100422_612GNAAXX:7:89:9710:20652#0/2_CONS_SUB_SUB count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=99; obiclean_headcount=0; seq_rank=36; obiclean_samplecount=1; obiclean_head=False; +TTAGCCCTAAACATAAACATTCAATAAACAAGAATGTTCGCCAGAGTACTACTAGCAACA +GCCTGAAACTCAAAGGACTTGGCGGTGCTTTATATCCCT +>HELIUM_000100422_612GNAAXX:7:71:17473:7401#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'15a_F730814': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=37; obiclean_samplecount=1; obiclean_head=False; +TTAGCCCTAAACACAAGTAATTAATATAACAAAATTATTCGCCAGAGTACTACCGGTAAT +AGCTTAAAACTCAAAGGACTTGGCGGTGCTTTATACCCTT +>HELIUM_000100422_612GNAAXX:7:115:3625:10051#0/2_CONS_SUB_SUB count=1; merged_sample={'13a_F730603': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=5; obiclean_headcount=0; seq_rank=38; obiclean_samplecount=1; obiclean_head=True; +CAATA +>HELIUM_000100422_612GNAAXX:7:99:8183:13912#0/2_CONS_SUB_SUB count=1; merged_sample={'13a_F730603': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=39; obiclean_samplecount=1; obiclean_head=False; +CTAGCCTTAAACACAAATAGTTATGCAAACAAAACTATTCGCCAGAGTACTACCGGCAAT +AGCTTAAAACTCAAAGGACTTGGCGGTGCTTTATACCCTC +>HELIUM_000100422_612GNAAXX:7:22:1798:3790#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'13a_F730603': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=40; obiclean_samplecount=1; obiclean_head=False; +CTAGCCTTAAACACAAATAGTTATGCAAACAAAACTATTCGCCAGAGTGCTACCGGCAAT +AGCTTAAAACTCAAAGGACTTGGCGGTGCTTTATACCCTT +>HELIUM_000100422_612GNAAXX:7:115:14354:10118#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=99; obiclean_headcount=0; seq_rank=41; obiclean_samplecount=1; obiclean_head=False; +TTAGCCCTAAACATAAACATTCAATAAACAAGAATGTTCGCCAGAGTACTACTCGCAACA +GCCTGAAACTCAAAGGACTTGGCGGTGCTTTACATCCCT +>HELIUM_000100422_612GNAAXX:7:103:3594:15996#0/2_CONS_SUB_SUB count=1; merged_sample={'13a_F730603': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=42; obiclean_samplecount=1; obiclean_head=False; +CTAGCCTTAAACACAAATAGTTATGCAAACACAACTATTCGCCAGAGTACTACCGGCAAT +AGCTTAAAACTCAAAGGACTTGGCGGTGCTTTATACCCTT +>HELIUM_000100422_612GNAAXX:7:105:14135:6905#0/2_CONS_SUB_SUB count=1; merged_sample={'29a_F260619': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=43; obiclean_samplecount=1; obiclean_head=True; +TTAGCCCTAAACACAAGTAATTAATATAACAAAATTATTCACCAGAGTACTAGCGGCAAC +AGCTTAAAACTCAAAGGACTTGGCGGTGCTTTATACCCTT +>HELIUM_000100422_612GNAAXX:7:76:18268:14980#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'29a_F260619': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=44; obiclean_samplecount=1; obiclean_head=False; +TTACCCCTAAACACAAGTAATTAATATAACAAAATTATTCGCCAGAGTACTACCGGCAAT +AGCTTAAAACTCAAAGGACTTGGCGGTGCTTTATACCCTT +>HELIUM_000100422_612GNAAXX:7:25:7789:3090#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'29a_F260619': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=45; obiclean_samplecount=1; obiclean_head=False; +TTAGCCCTAAACACAAGTAATTAATATAACAAAATTATTCGCCAGAGTACTACAGGCAAT +AGCTTAAAACTCAAAGGACTTGGCGGTGCTTTATACCCTT +>HELIUM_000100422_612GNAAXX:7:42:19757:3800#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'15a_F730814': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=99; obiclean_headcount=0; seq_rank=46; obiclean_samplecount=1; obiclean_head=False; +TTAGCCCTAAACACAAGTAATTAATATACAAAATTATTCGCCAGAGTACTACCGGCAATA +GCTTAAAACTCAAAGGACTTGGCGGTGCTTTATACCCTT +>HELIUM_000100422_612GNAAXX:7:116:14244:17926#0/2_CONS_SUB_SUB count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=99; obiclean_headcount=0; seq_rank=47; obiclean_samplecount=1; obiclean_head=False; +TTAGCCCTAAACATAAACATTCAATAAACAAGAATGTTCGCAAGAGTACTACTAGCAACA +GCCTGAAACTCAAAGGACTTGGCGGTGCTTTACATCCCT +>HELIUM_000100422_612GNAAXX:7:72:12959:5736#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=99; obiclean_headcount=0; seq_rank=48; obiclean_samplecount=1; obiclean_head=False; +TTAGCCCTAAACATAAACATTCAATAAACAAGAATGTTCGCCAGAGTACTACTAGCAACA +GCCTGAAACTCAAAGGACTTGGCGGTGCTTTACACCCCT +>HELIUM_000100422_612GNAAXX:7:7:15538:8325#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=99; obiclean_headcount=0; seq_rank=49; obiclean_samplecount=1; obiclean_head=True; +TTAGCCCTAAACATAAACATTCAATAAACAAGAATGTCGGCCAGAGTACTACTAGCAACA +GCATGAAACTCAAAGAACTGGGCGGTGCTTTACATCCCT +>HELIUM_000100422_612GNAAXX:7:120:16005:7955#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=98; obiclean_headcount=0; seq_rank=50; obiclean_samplecount=1; obiclean_head=False; +TTAGCCCTAAACATAAACATTCAATAAACAAGAATGTTCGCCAGAGTACTACTAGCAACA +GCCGAAACTCAAAGGACTTGGCGGTGCTTTACATCCCT +>HELIUM_000100422_612GNAAXX:7:10:9237:10532#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'29a_F260619': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=51; obiclean_samplecount=1; obiclean_head=False; +TTAGCCCTAAACACAAGTAATTAATATAACAAAATAATTCGCCAGAGTACTACCGGCAAT +AGCTTAAAACTCAAAGGACTTGGCGGTGCTTTATACCCTT +>HELIUM_000100422_612GNAAXX:7:7:11998:4462#0/2_CONS_SUB_SUB count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=99; obiclean_headcount=0; seq_rank=52; obiclean_samplecount=1; obiclean_head=False; +TTAGCCCTAAACATAAACATTCAATAAACAAGAATGTTCGCCAGAGTAGTACTAGCAACA +GCCTGAAACTCAAAGGACTTGGCGGTGCTTTACATCCCT +>HELIUM_000100422_612GNAAXX:7:111:18277:17779#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=99; obiclean_headcount=0; seq_rank=53; obiclean_samplecount=1; obiclean_head=True; +TTAGCCCTAAACATAGATAATTTTACAACAAAATAATTCGCCAGAGGACTACTAGCAATA +GCCTGAAACTCAAAGGACTTGGCGGTGCTTTACATCCCT +>HELIUM_000100422_612GNAAXX:7:3:17077:6562#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'15a_F730814': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=54; obiclean_samplecount=1; obiclean_head=False; +TTAGCCCTAAACACAAGTAATTAATATAACAAAATTATTCGCCAGAGTACTGCCGGCAAT +AGCTTAAAACTCAAAGGACTTGGCGGTGCTTTATACCCTT +>HELIUM_000100422_612GNAAXX:7:19:4311:13343#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=99; obiclean_headcount=0; seq_rank=55; obiclean_samplecount=1; obiclean_head=False; +TTAGCCCTAAACATAAACATTCAATAAACAAGAATGTTCGCCAGAGTACTACGAGCAACA +GCCTGAAACTCAAAGGACTTGGCGGTGCTTTACATCCCT +>HELIUM_000100422_612GNAAXX:7:30:1800:7833#0/2_CONS_SUB_SUB count=1; merged_sample={'15a_F730814': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=56; obiclean_samplecount=1; obiclean_head=True; +TTAGCCCTAAACACAAGACATTAATATAACGAGATTAATCGACAGAGTACTACCGGCTAT +AGCTTAAAACTCAAAGGACTTGGCGGTGCTTTATACCCTT +>HELIUM_000100422_612GNAAXX:7:108:9222:18258#0/2_CONS_SUB_SUB count=1; merged_sample={'13a_F730603': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=57; obiclean_samplecount=1; obiclean_head=False; +CTAGCCTTAAACACAAATAGTTATGCAGACAAAACTATTCGCCAGAGTACTACCGGCAAT +AGCTTAAAACTCAAAGGACTTGGCGGTGCTTTATACCCTT +>HELIUM_000100422_612GNAAXX:7:38:3005:20881#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=99; obiclean_headcount=0; seq_rank=58; obiclean_samplecount=1; obiclean_head=False; +TTAGCCCTAAACATGAACATTCAATAAACAAGAATGTTCGCCAGAGTACTACTAGCAACA +GCCTGAAACTCAAAGGACTTGGCGGTGCTTTACATCCCT +>HELIUM_000100422_612GNAAXX:7:29:15520:18035#0/2_CONS_SUB_SUB count=2; merged_sample={'29a_F260619': 2}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=7; obiclean_samplecount=1; obiclean_head=True; +TTAGCCCTAAACACAAGTAATTATTATAACAAAATTATTCGCCAGAGTACTACCGGCAAT +AGCTTAAAACTCAAAGGACTTGGCGGTGCTTTATACCCTT +>HELIUM_000100422_612GNAAXX:7:115:17402:4174#0/2_CONS_SUB_SUB_CMP count=2; merged_sample={'29a_F260619': 1, '15a_F730814': 1}; obiclean_singletoncount=0; obiclean_internalcount=2; seq_length=100; obiclean_headcount=0; seq_rank=13; obiclean_samplecount=2; obiclean_head=False; +TTAGCCCTAAACACAAGTAATTAATATAACAAAATTATTCGCCAGAGTACTACCTGCAAT +AGCTTAAAACTCAAAGGACTTGGCGGTGCTTTATACCCTT +>HELIUM_000100422_612GNAAXX:7:38:10813:16867#0/2_CONS_SUB_SUB count=2; merged_sample={'26a_F040644': 2}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=99; obiclean_headcount=0; seq_rank=15; obiclean_samplecount=1; obiclean_head=False; +TTAGCCCTAAACATAAACATTCAATAAACAAGAATGTTTGCCAGAGTACTACTAGCAACA +GCCTGAAACTCAAAGGACTTGGCGGTGCTTTACATCCCT +>HELIUM_000100422_612GNAAXX:7:70:11798:2668#0/2_CONS_SUB_SUB count=2; merged_sample={'15a_F730814': 2}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=20; obiclean_samplecount=1; obiclean_head=True; +TTAGCCCTAAACACAAGTAATTAATATAACAAAATTATTCGCCAGAGTACTACCGGCAAT +AGCTTAAAACTCAAAGGACTTGGCGGTGCTTTATACCTTT +>HELIUM_000100422_612GNAAXX:7:89:10281:5930#0/2_CONS_SUB_SUB_CMP count=3; merged_sample={'29a_F260619': 3}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=6; obiclean_samplecount=1; obiclean_head=True; +TTAGCCCTAAACACAAATAATTACACAAACAAAATTGTTCACCAGAGTACTAGCGGCAAC +AGCTTAAAACTCAAAGGACTTGGCGGTGCTTTATACCCTT +>HELIUM_000100422_612GNAAXX:7:13:5742:9859#0/2_CONS_SUB_SUB count=7; merged_sample={'29a_F260619': 7}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=4; obiclean_headcount=0; seq_rank=16; obiclean_samplecount=1; obiclean_head=True; +TTTT +>HELIUM_000100422_612GNAAXX:7:6:9274:14951#0/2_CONS_SUB_SUB count=46; merged_sample={'13a_F730603': 46}; obiclean_singletoncount=0; obiclean_internalcount=0; seq_length=100; obiclean_headcount=1; seq_rank=8; obiclean_samplecount=1; obiclean_head=True; +CTAGCCTTAAACACAAATAGTTATGCAAACAAAACTATTCGCCAGAGTACTACCGGCAAT +AGCTTAAAACTCAAAGGACTTGGCGGTGCTTTATACCCTT +>HELIUM_000100422_612GNAAXX:7:22:8540:14708#0/2_CONS_SUB_SUB count=61; merged_sample={'29a_F260619': 30, '15a_F730814': 31}; obiclean_singletoncount=0; obiclean_internalcount=0; seq_length=100; obiclean_headcount=2; seq_rank=4; obiclean_samplecount=2; obiclean_head=True; +TTAGCCCTAAACACAAGTAATTAATATAACAAAATTATTCGCCAGAGTACTACCGGCAAT +AGCTTAAAACTCAAAGGACTTGGCGGTGCTTTATACCCTT +>HELIUM_000100422_612GNAAXX:7:57:18459:16145#0/2_CONS_SUB_SUB_CMP count=64; merged_sample={'26a_F040644': 64}; obiclean_singletoncount=0; obiclean_internalcount=0; seq_length=99; obiclean_headcount=1; seq_rank=5; obiclean_samplecount=1; obiclean_head=True; +TTAGCCCTAAACATAAACATTCAATAAACAAGAATGTTCGCCAGAGTACTACTAGCAACA +GCCTGAAACTCAAAGGACTTGGCGGTGCTTTACATCCCT diff -r 000000000000 -r aca6a8c9cf83 test-data/output_obigrep_lmin.fastq --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/output_obigrep_lmin.fastq Wed Apr 12 17:38:00 2017 -0400 @@ -0,0 +1,32 @@ +@HELIUM_000100422_612GNAAXX:7:13:5742:9859#0/2_CONS ali_length=58; direction=right; seq_ab_match=55; sminR=40.0; seq_a_mismatch=3; seq_b_deletion=50; seq_b_mismatch=0; seq_a_deletion=50; seq_b_single=0; score=207.846895043; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=0; score_norm=3.58356715591; seq_b_insertion=0; +ccgcctcctttagataccccactatgcttttctagaggagcctgttctaaggaggcgg ++ +~~~~~~Z~~~~~~~~~b~~~~~~~~~_~~~~~~~~~~}~~~~~~~~~~~~~~~~~~~~ +@HELIUM_000100422_612GNAAXX:7:7:7916:16505#0/2_CONS ali_length=58; direction=right; seq_ab_match=58; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=50; seq_b_mismatch=0; seq_a_deletion=50; seq_b_single=0; score=231.999536374; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=0; score_norm=3.99999200644; seq_b_insertion=0; +ccgcctccttagaacaggctcctctagaaaagcatagtggggtatctaaaggaggcgg ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@HELIUM_000100422_612GNAAXX:7:37:12979:6595#0/2_CONS ali_length=58; direction=right; seq_ab_match=58; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=50; seq_b_mismatch=0; seq_a_deletion=50; seq_b_single=0; score=231.998796685; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=0; score_norm=3.99997925319; seq_b_insertion=0; +ccgcctcctttagataccccactatgcttttctagaggagcctgttctaaggaggcgg ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@HELIUM_000100422_612GNAAXX:7:96:8737:4973#0/2_CONS ali_length=58; direction=right; seq_ab_match=57; sminR=40.0; seq_a_mismatch=1; seq_b_deletion=50; seq_b_mismatch=0; seq_a_deletion=50; seq_b_single=0; score=223.980140585; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=0; score_norm=3.86172656181; seq_b_insertion=0; +ccgcctccttagaacaggctcctctagaaaagcatagtggggtatctaaaggaggcgg ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@HELIUM_000100422_612GNAAXX:7:115:3625:10051#0/2_CONS ali_length=59; direction=right; seq_ab_match=59; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=49; seq_b_mismatch=0; seq_a_deletion=49; seq_b_single=0; score=235.999642017; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=0; score_norm=3.99999393249; seq_b_insertion=0; +ccaattaacttagataccccactatgccaatactagaggagcctgttctagttaattgg ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@HELIUM_000100422_612GNAAXX:7:73:17068:16620#0/2_CONS ali_length=58; direction=right; seq_ab_match=57; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=50; seq_b_mismatch=1; seq_a_deletion=50; seq_b_single=0; score=223.962007947; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=0; score_norm=3.86141393012; seq_b_insertion=0; +ccgcctccttagaacaggctcctctagaaaagcatagtggggtatctaaaggaggcgg ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~|~ +@HELIUM_000100422_612GNAAXX:7:95:11283:2557#0/2_CONS ali_length=58; direction=right; seq_ab_match=57; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=50; seq_b_mismatch=1; seq_a_deletion=50; seq_b_single=0; score=223.999078974; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=0; score_norm=3.86205308576; seq_b_insertion=0; +ccgcctccttagaacaggctcctctagaaaagcatagtggggtatctaaaggaggcgg ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~a~~~~~~~~~~~~~~~ +@HELIUM_000100422_612GNAAXX:7:75:9674:16966#0/2_CONS ali_length=58; direction=right; seq_ab_match=57; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=50; seq_b_mismatch=1; seq_a_deletion=50; seq_b_single=0; score=224.000075421; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=0; score_norm=3.86207026589; seq_b_insertion=0; +ccgcctccttagaacaggctcctctagaaaagcatagtggggtatctaaaggaggcgg ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~~~ diff -r 000000000000 -r aca6a8c9cf83 test-data/output_obigrep_predicat.fasta --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/output_obigrep_predicat.fasta Wed Apr 12 17:38:00 2017 -0400 @@ -0,0 +1,980 @@ +@HELIUM_000100422_612GNAAXX:7:119:14871:19157#0/2_CONS ali_length=62; direction=left; seq_ab_match=47; sminR=40.0; seq_a_mismatch=1; seq_b_deletion=1; seq_b_mismatch=13; seq_a_deletion=0; seq_b_single=46; score=115.761290673; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=1.86711759151; seq_b_insertion=1; +ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttgagctattgccggtagtactctggcgaatgattttgttataataattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg ++ +dddddddddddbbdaddcddddddcadaddddaadcccWaaddabdx~~|b~~~~cccccccBcBcccBcBcccBccBBcc~}`kXyxu|~Kyr`b~~~~~~b~~~b~d\cdddddddddddddcacddddcdddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:108:5640:3823#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=2; seq_a_deletion=0; seq_b_single=46; score=231.881364714; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.74002201152; seq_b_insertion=0; +ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaacaattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg ++ +ddddddddddddddddbdddddbddddddddddddddddddddddd~~~~~~~~~~~~~~~~~~}~~~~~~~~~~~~~~~~~~~~~~~~~~~Z~~~~~~~~~~~~~b~d]dddddddcdddddcddddddddddddddddddddddcddddddd +@HELIUM_000100422_612GNAAXX:7:97:14311:19299#0/2_CONS ali_length=62; direction=left; seq_ab_match=52; sminR=40.0; seq_a_mismatch=5; seq_b_deletion=0; seq_b_mismatch=5; seq_a_deletion=0; seq_b_single=46; score=167.91734192; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=2.70834422451; seq_b_insertion=0; +ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctcttgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg ++ +ddadddddcd`dcddddddddd^dd]ddddddZad`\`]Lcca``YT|~~^||v|z|~ts~~~|r~sZ^~~`~~~~~~~~~~~~~~~~V~b~]~~~~b~~~~~~~~`~b\aadddd`dcdddddc`dddccddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:22:8540:14708#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=3; seq_a_deletion=0; seq_b_single=46; score=223.982011431; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.61261308759; seq_b_insertion=0; +ccgcctcctttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctaaggaggcgg ++ +dddedddddddddedddddddddedddaddbddddddddddddedd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~b~~~~~~~~b~~~~ddddddddddabbb^bdddddddddddcddddddddcddddddddd +@HELIUM_000100422_612GNAAXX:7:57:18459:16145#0/2_CONS ali_length=63; direction=left; seq_ab_match=60; sminR=40.0; seq_a_mismatch=3; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=45; score=227.883189945; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.61719349119; seq_b_insertion=0; +ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +ddcdddddddddddddddeddddddddddddddddddddcddddd~b~~~~~~b~~~~~~~~~~~~~~c~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:89:10281:5930#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=231.893859399; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.7402235387; seq_b_insertion=0; +ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctgttgccgctagtactctggtgaacaattttgtttgtgtaattatttgtgtttagggctaagcatagtggggtatctaaaggaggcgg ++ +ddddddddddddddddddcddddddddddddd\ddeddecddddddb~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddcddddddcdddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:29:15520:18035#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; seq_b_single=46; score=239.965360923; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.87040904714; seq_b_insertion=0; +ccgcctcctttagataccccactatgcttagccctaaacacaagtaattattataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctaaggaggcgg ++ +cccccccc_cc_cccacacb]Y]_`a^__`_Y_`_a_`cc_ccccc~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~dddddddddddddddddddddddddddadddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:6:9274:14951#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=2; seq_a_deletion=0; seq_b_single=46; score=223.791171196; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.60953501929; seq_b_insertion=0; +ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg ++ +dcdddddddddddedddddddddcddddddcddddddddddddddd~~~~~~~~~~~~~~~~~~|z~}a|~~~~}}}}~~~~~~~~~~}~}b~b~~~~~~~~~~~~~~ddddddcdcddcd^ddddddddddddddddddddddeddddddddd +@HELIUM_000100422_612GNAAXX:7:111:2893:6772#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=239.959941063; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.87032163005; seq_b_insertion=0; +ccgcctcctttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctaaggaggcgg ++ +dddddddddddddddddddddddddddddddddddedddddded_d~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~x~dddddddcdddcdcdddadddddddddddedddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:89:10621:20317#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=3; seq_a_deletion=0; seq_b_single=46; score=223.827654468; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.61012345916; seq_b_insertion=0; +ccgcctcctttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctaaggaggcgg ++ +ddddddddddddddbdddddadddddddd`ddddadadddddddRc~}~~z~~~~~~~t~sw}~~g}~~~~~~~~~~~~~~~~~~~~~~~~~~~~^~b`~~~~~~~~~ddddddddbdbdd_dddadddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:50:2791:11138#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=231.976017167; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.74154866398; seq_b_insertion=0; +ccgcctcctttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctaaggaggcgg ++ +dddddcdddddcddddcd^dddddddddbdbdddddddc\ddddbd~~~~~~~~~~~~~~~~~~b~~~^~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddbdddddddddcddddddddcddddddddddddd +@HELIUM_000100422_612GNAAXX:7:108:17701:18887#0/2_CONS ali_length=62; direction=left; seq_ab_match=58; sminR=40.0; seq_a_mismatch=4; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=215.828321765; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.48110196396; seq_b_insertion=0; +ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg ++ +dd[dddddddd``ddddddddddddeddddddcdddddd\ddddddb~~~b~~~~~~~~~~~b~~~~~b~~~{~~{z|{|~~~~~~~~~~~ssx~~~~~~~~~~~~~~c^cddddddddddd`cedddddddddddddddddddddddddddcd +@HELIUM_000100422_612GNAAXX:7:97:10414:9929#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=239.864541102; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.86878292101; seq_b_insertion=0; +ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg ++ +ddddddddddddddddddddddddddddddddcddddddcdddddd~~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddeddddddddddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:97:9091:15775#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; seq_a_mismatch=3; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=223.861916171; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.61067606728; seq_b_insertion=0; +ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg ++ +ddddddddddddddddddddddddddddddddcddddddddddddd~~~~b~~^~~~~~~b~~~~~~~|~~~~~~~~~x~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddddadcdddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:74:8683:20738#0/2_CONS ali_length=63; direction=left; seq_ab_match=62; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; seq_b_single=45; score=243.961428864; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.87240363276; seq_b_insertion=0; +tcgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +ddddddddddddddddddddddddddddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~`~~~~~~~~~~~~~~ddedddddddddddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:24:16230:12703#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; seq_b_single=46; score=223.86908915; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.61079176049; seq_b_insertion=0; +ccgaagtagtagaacaggctcctctagaaggggataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg ++ +ddd`ddddad_ddaddddddddaddddddc`bO``ddd_a`ddd`a}}~sb{~~~~~~~~~~~~~~~~a~~~~~~y~u~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~b^ddcdbcddadddd_ddbdcdcdddd`ddddbddddddddddddd +@HELIUM_000100422_612GNAAXX:7:4:4214:9434#0/2_CONS ali_length=62; direction=left; seq_ab_match=58; sminR=40.0; seq_a_mismatch=3; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; seq_b_single=46; score=215.812703412; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.48085005502; seq_b_insertion=0; +ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactacaggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg ++ +ddddddddSddddbbdddddddbddabdddbdddddcddddddddd~^~~~{~{~~~~~}~~{~~ym~L||~~wuy~zyxPzjyyvxku~~~~~~~~~~~~~~~~~xbacdddddaddac_^`_`dcddddacddcddddadaa`[``^c`acc +@HELIUM_000100422_612GNAAXX:7:12:17693:3451#0/2_CONS ali_length=62; direction=left; seq_ab_match=62; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=247.862243; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.9977781129; seq_b_insertion=0; +ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg ++ +dddddddddddddddddddddddddbddddddaddddddcdddddc~~~~~~~~~~~~~~~~~~~~~~~~~}~{~}{v}~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddeddddddddddcdeddddddcddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:3:14624:12375#0/2_CONS ali_length=63; direction=left; seq_ab_match=61; sminR=40.0; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; seq_b_single=45; score=235.922125108; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.74479563663; seq_b_insertion=0; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +ddddddddddddddddddcdcddddcddcdcdddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~Y`~~~~~~~~~~~~~~~~~~~~~~~dddddcdddddddcedddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:18:5317:11571#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=239.862078449; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.86874320079; seq_b_insertion=0; +ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg ++ +ddddddddddddddddddddddddddddddddadddddddcddddd~~~~b~~~~~~~~~~{~~~~~~~~~~~~~~~~~y~~~~~~~~~~~~~~~~~~~~~~~~~~~~edddeddddddddddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:109:17335:11429#0/2_CONS ali_length=63; direction=left; seq_ab_match=56; sminR=40.0; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=6; seq_a_deletion=0; seq_b_single=45; score=195.716358124; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.10660885911; seq_b_insertion=0; +ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +ddddcdddddcdddddda`ddd^dd`^^`bdd`dR\]``]cdddd~x}~~W}}~~}~~~~~x~s~~z~ccccccccccccccccc~[|~`\~}``~|~~~~~~~~~b~dbdcddddddddadcdddcdcdddcddddaddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:7:10646:8487#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; seq_b_single=46; score=231.85443709; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.739587695; seq_b_insertion=0; +ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg ++ +dddddddddddddddddddddddddddcdddddddddddddddddd~~~~~~~~~~~~~~~~b~~~~~~~hpoppitzv{~~~~~~~~~~~~~~~~~~~~~~~~~~~cedddddddddddddddccdddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:19:4865:20715#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=231.923942025; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.74070874234; seq_b_insertion=0; +ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg ++ +ddddddddddddddddddddddddddddcdddaddcddddddddddby~~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~bddddddddddddddbcdddddeddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:13:6954:13039#0/2_CONS ali_length=63; direction=left; seq_ab_match=62; sminR=40.0; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=45; score=243.872474161; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.87099165336; seq_b_insertion=0; +ccgaatatctagaacaggctcctctagagggatataaagcaccgccaagtcctttgagttttaagctattgctagtagtcctctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +dddddddddddddddddddddddddddddddcddddddddddddd~~~~~b~~~~~~~~~~~~~~~~~~~~~~~r~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddcdddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:120:14963:1929#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; seq_b_single=46; score=223.810433242; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.60984569745; seq_b_insertion=0; +ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg ++ +ddddddddddddddddddddddddddddddddcddddddddddddd~~~~_~~~~~~~~~~~~~~}~~~}}`z}~~~}~~}~~|~~u~~~~~~~~~~~~~~~~}~~b~dbdddddedddddddadddcddddcddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:115:3921:10649#0/2_CONS ali_length=63; direction=left; seq_ab_match=61; sminR=40.0; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; seq_b_single=45; score=235.871015095; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.74398436659; seq_b_insertion=0; +ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +dddddddddddddddddddddddeddddddddddddddddddddd~b~~~~~~~~~~~~~~~~~~~~~~i~{n~}~}~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~dddddcdddddddddeddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:109:12848:8882#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=231.869965661; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.73983815582; seq_b_insertion=0; +ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg ++ +ddeddcddddddcdddddddddddddddddddTdddddddddddcdb~~~~~~~~~~~~~~~~~~~~~~}~~~b~x~~{~~~~~~~~~~~~~~~~~~~~~~~~~~~~~d`dcdddddddddddeeddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:84:11257:18886#0/2_CONS ali_length=63; direction=left; seq_ab_match=55; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=8; seq_a_deletion=0; seq_b_single=45; score=187.890540948; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=2.98238953885; seq_b_insertion=0; +ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +dddddddddddddddddddddddddddcddddddddddddddddd~t~~x~~~~~~~~~~~~~~~~~~~~t~~~~~~y~s}\`~~a|~b{~~~~~~b~~~~b~~a~b~ddddddddddddddcdddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:72:13060:5051#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=239.985823237; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.87073908447; seq_b_insertion=0; +ccgcctcctttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctaaggaggcgg ++ +ddbddddddddcbbdddcdcdddbd`cddddddddddddddddd_b~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddedddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:35:3798:1896#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=239.863223783; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.86876167392; seq_b_insertion=0; +ccgaagtagttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctactacttcgg ++ +dddddddddddddbddddddddddddddddddddcddbddddddad~b~~}~~~~~~~~~~~~~~~~~n~~~~~|~}~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddcddaddaddd^dddbcddddddddadddddddddcdddddddd +@HELIUM_000100422_612GNAAXX:7:117:12470:18434#0/2_CONS ali_length=63; direction=left; seq_ab_match=59; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=4; seq_a_deletion=0; seq_b_single=45; score=219.83660627; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.4894699408; seq_b_insertion=0; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +ddddddcddddddbdddcdddddbbdddddddddddcddddddcd~~~~~~~~~~~~~~~~~~~~~~~~~~~~O~~~~~~~~~{~~{~~Wx~~~~~~~~~~~b~~~b}ddabdddddddddddddddddddddddddddddddeddddddbdd +@HELIUM_000100422_612GNAAXX:7:11:8428:13716#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=239.97387484; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.87054636839; seq_b_insertion=0; +ccgaagtagttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctactacttcgg ++ +ddddddddddddddddddddddddddddddddddddddddddddad~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddddddddddddddddcddddeddddddddddddd +@HELIUM_000100422_612GNAAXX:7:78:18994:16521#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=239.927346216; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.86979590672; seq_b_insertion=0; +ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg ++ +ddcdddddcdddcdddcdddddddddcbdddd`dedddaeeddaddb~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dadddddddddcddddddcddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:16:5047:7843#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=45; score=251.979285107; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.99967119217; seq_b_insertion=0; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +ddddddddddddddddddddddddddddedddbdddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddcdcddddddeeccdddddddcedddddeddddddddddd +@HELIUM_000100422_612GNAAXX:7:118:13803:15594#0/2_CONS ali_length=63; direction=left; seq_ab_match=60; sminR=40.0; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; seq_b_single=45; score=227.87720055; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.61709842144; seq_b_insertion=0; +ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +ddddddddddddddddddddddddddddddddddddddddddddd~~~~~b~~b~~~~~~~~~~v~~~~~~~~~~y~{~~~~~~~~~~~a~~~~~~~~~~~~~~~~~~dddedddddbdddd^ddeddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:53:14379:5922#0/2_CONS ali_length=62; direction=left; seq_ab_match=62; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=247.982071703; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.99971083392; seq_b_insertion=0; +ccgcctcctttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctaaggaggcgg ++ +dddcdddddddddddddddddddddddddddddddddddddddd`d~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddeddddddddddddddddddcddddddddddddd +@HELIUM_000100422_612GNAAXX:7:52:10700:16508#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=239.790995416; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.86759670025; seq_b_insertion=0; +ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg ++ +dddddddddddddeddddcdddcdddddddddIddbdddbdcddddb~~u~~~}~|||v|~~~~{~~~~~}}}~~~~~~~~~~~~~~~~v~~{{~~~~~~~~~~~~~~ccccccacccccccccc^cccccccccccccccc_cccccc[cca^ +@HELIUM_000100422_612GNAAXX:7:69:7696:12881#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=45; score=251.983052061; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.99973098509; seq_b_insertion=0; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +dddddddddddddddddddddddddddeddddddcdddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddcdddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:44:12484:5320#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=231.837799589; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.73931934821; seq_b_insertion=0; +ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg ++ +ddddcbddddddccddddeddddddddddddddddddddaddddcda~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~z}}}~~~~~~~~~~~~~~~~~~~cddddddddddddddddeddddddddddddddddddddddeddddd +@HELIUM_000100422_612GNAAXX:7:21:4713:10229#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=239.864510201; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.86878242259; seq_b_insertion=0; +ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg ++ +dddddddddddddddddddddddddddbdddd`ddddddddddddd~~~~~~~~~~~~~~b~~~~~~~~~~~~~~~~~~~|~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddcddddddddbdcdddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:38:6201:12003#0/2_CONS ali_length=63; direction=left; seq_ab_match=59; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=4; seq_a_deletion=0; seq_b_single=45; score=219.686655506; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.48708976993; seq_b_insertion=0; +ccgaatatctagaacaggctcctctagcgggatataaagcaccgcccagttctttgagttttaagctattgctagtagtcctctggcgaattattttgttgtaaaattatctatgtttagggctaagcatagtggggtatctaagatattcgg ++ +bbb`^a``b[a[aa^YVY\YW__TXbbHaDDDDDDDDDDDDDDDDcBcccBccccccccccccccccc~x~z}}}}}y~rzwzz||{zyzxa{}~t~~~~~~~b~~~~cddddd[dddcddcddbddbdddddd`dddd^dcddddddddddd +@HELIUM_000100422_612GNAAXX:7:33:16506:1490#0/2_CONS ali_length=62; direction=left; seq_ab_match=62; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=247.963002972; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.99940327375; seq_b_insertion=0; +ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg ++ +ddddeddddddddddddddddddddddddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddeddddddddddddddddddddddddddeedddd +@HELIUM_000100422_612GNAAXX:7:1:9007:3289#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=231.83380127; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.7392548592; seq_b_insertion=0; 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+ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg ++ +ddddddddddddddddddddddddddddddcddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddedddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:117:4291:18111#0/2_CONS ali_length=63; direction=left; seq_ab_match=62; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; seq_b_single=45; score=243.911625561; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.87161310415; seq_b_insertion=0; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +dddddddddddddddddddddddddddddddddddaddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~ddddddddddddddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:71:14800:3222#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=231.89389474; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.74022410871; seq_b_insertion=0; +ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg ++ +ddddddddddddddddddeddddddddeddddcdddddccdddddd~~~~b~~~~~~~~~~~~~~~~~\~~~}~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dcddddddddeedddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:11:8547:18238#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=45; score=251.984413754; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.99975259927; seq_b_insertion=0; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +ddddddddddddddddddddddddddddbdcdddddddddcdddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddddddddddddddddddddeddddddddddddd +@HELIUM_000100422_612GNAAXX:7:117:2416:10342#0/2_CONS ali_length=63; direction=left; seq_ab_match=61; sminR=40.0; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=45; score=235.921215944; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.74478120546; seq_b_insertion=0; +ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +dddddddddddddddcdcddddaddcddddddddbddddbddddd~b~~|b~~~~~}~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddcdcddddY[ZYUd^dbdbdddddddddddddcdddddddddd +@HELIUM_000100422_612GNAAXX:7:78:7151:19928#0/2_CONS ali_length=63; direction=left; seq_ab_match=56; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=7; seq_a_deletion=0; seq_b_single=45; score=195.576462373; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.10438829164; seq_b_insertion=0; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacgagaatgttcgccagtgtacttctagcaacaggctgaaactcaaaggacttggcggtggtttacatccctctagaggagcctgttctagatattcgg ++ +ZYQOYVVXRYYOYZZ^^^_bZZ]Z[S[WSR]YbbbDDDDDDDDDDcccccccccccBBcccccccccccccBcccccBccccccccccBccccccccccBccccc~`~WNS]Y_KTUVZ[O_SHW]ZQ_^^^ab`Obb\TY\MPPPPQQQQQQ +@HELIUM_000100422_612GNAAXX:7:71:18821:20471#0/2_CONS ali_length=62; direction=left; seq_ab_match=53; sminR=40.0; seq_a_mismatch=7; seq_b_deletion=0; seq_b_mismatch=2; seq_a_deletion=0; seq_b_single=46; score=175.889514148; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=2.83692764755; seq_b_insertion=0; +ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg ++ +ddddddddddddddddddddddddddddd\ddddddddd^ddd`dd~~~~Xx~~~~~{|q}}x}^Y`J`~Xv{wt~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~sbddddddddddddd^bdccddbdddddd`ddddddddddddddcddd +@HELIUM_000100422_612GNAAXX:7:58:15837:8531#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=45; score=251.96128051; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.99938540492; seq_b_insertion=0; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +ddddddddddddddddddddddddddddddbdddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddcdddddddddddddcdddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:38:10813:16867#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=45; score=251.97777927; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.99964729; seq_b_insertion=0; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgtttgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +dddddddddddddddddddddddcdddddddddddeddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dbcdddddadadcdddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:68:19115:18847#0/2_CONS ali_length=62; direction=left; seq_ab_match=54; sminR=40.0; seq_a_mismatch=6; seq_b_deletion=0; seq_b_mismatch=2; seq_a_deletion=0; seq_b_single=46; score=183.856571062; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=2.96542856552; seq_b_insertion=0; +ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg ++ +cdbdddddddcdbcddc[Tabcddd_`bddaaabd^bb^P`ccd`db{~~b~~~~~~\\~~x~~~~s~~s~~~_~~{~~~~~~~b{~~}~~~~~~~~~~~b~~~~~`~ddd^dd_ddcdd_ddceddedcdddddcddacdddddddddddcad +@HELIUM_000100422_612GNAAXX:7:14:3978:11028#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=231.869517243; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.73983092328; seq_b_insertion=0; +ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgcaggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg ++ +ddcddddddcdddeddddddddcddddddcb`Zbbddddddddddd_~~~~~~~~~~~~~~~~~~~v~~~y~J|~u{|w~~~~~~~~~~~~~~~~~~~~~~~~~~~~~da`dddeddddcdbdaeddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:34:18733:3827#0/2_CONS ali_length=63; direction=left; seq_ab_match=62; sminR=40.0; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=45; score=243.983236898; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.87274979204; seq_b_insertion=0; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +ddddddddddddddddddddddddddddddddddddddddddddd~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:65:6787:8456#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=231.869006954; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.7398226928; seq_b_insertion=0; +ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg ++ +ddddddddbddddeddddedddddddddddddddddddddddddddb~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~d_ddeddddddcdddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:111:7441:14688#0/2_CONS ali_length=63; direction=left; seq_ab_match=62; sminR=40.0; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=45; score=243.832560428; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.87035810203; seq_b_insertion=0; +ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +ddddddddddddddddddddddddddddddddddddddddddddd~~~~~b~~~~~u~~~~~~~~~~~~~~~~~~}~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddedddddddddadddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:86:10092:15563#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; seq_b_single=46; score=223.927744132; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.61173780858; seq_b_insertion=0; +ccgcctcctttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctaaggaggcgg ++ +dddd`dddddbdddddddddddcddddddbdddddccdddddaddd~b~~~~~~~~~~~~~_~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~|bddddddddddcddcddd`^dddddddddddcddddddddddddbdd +@HELIUM_000100422_612GNAAXX:7:16:4418:5128#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; seq_a_mismatch=3; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=223.862543287; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.61068618206; seq_b_insertion=0; +ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg ++ +dddddddddddddddddddddddddddddddddddddddbddedddb~~~b~~b~~~~~~~~~~~~~~~{~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~bddddddddddddddedddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:13:5742:9859#0/2_CONS ali_length=58; direction=right; seq_ab_match=55; sminR=40.0; seq_a_mismatch=3; seq_b_deletion=50; seq_b_mismatch=0; seq_a_deletion=50; seq_b_single=0; score=207.846895043; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=0; score_norm=3.58356715591; seq_b_insertion=0; +ccgcctcctttagataccccactatgcttttctagaggagcctgttctaaggaggcgg ++ +~~~~~~Z~~~~~~~~~b~~~~~~~~~_~~~~~~~~~~}~~~~~~~~~~~~~~~~~~~~ +@HELIUM_000100422_612GNAAXX:7:85:18986:17662#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=2; seq_a_deletion=0; seq_b_single=46; score=231.874929984; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.73991822554; seq_b_insertion=0; +ccgaagtagttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctactacttcgg ++ +ddcdddddddddeccdddddddddcbdddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~}~~s~~~~~b~~~~~~b~~~~~~~ddcddddddcddddddddddddd`dddddddddddddddddddded +@HELIUM_000100422_612GNAAXX:7:16:16357:19948#0/2_CONS ali_length=63; direction=left; seq_ab_match=57; sminR=40.0; seq_a_mismatch=5; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; seq_b_single=45; score=203.860719115; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.23588443039; seq_b_insertion=0; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcacaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +baNdQdd`dd`da_ddbadbdddcddddd_dbddbdaddbddddd~`~~b~~~~~~b~~{~~~}x}}~}`twyhw~~~~}w|~|~~~~~X~~~|~~~~~Y~~~~~~~~XVRSQ[Qadd``^P^adYdddddddcc`^_dccddcdccdddddd +@HELIUM_000100422_612GNAAXX:7:107:3570:18147#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=231.924768153; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.74072206698; seq_b_insertion=0; +ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttatgctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg ++ +ddada`dcddddddddddddddadd_^b_cdd]dddddcdd_dcdd`~s~a~~~~~~~~}~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~d\d\dddddcdddddadddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:82:8836:11354#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=231.781218203; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.73840674522; seq_b_insertion=0; +ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg ++ +dddddddddddddddddddddddddddaddddcdddddcaddddddb~~~b~~~~~~~~~~~~~~~~~~~~~|~~k{{w~~~~~~~~~~~~~~~~~~~~~~{~~~~~~ddddddddddddddddeddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:41:2354:1449#0/2_CONS ali_length=63; direction=left; seq_ab_match=55; sminR=40.0; seq_a_mismatch=5; seq_b_deletion=0; seq_b_mismatch=3; seq_a_deletion=0; seq_b_single=45; score=187.777927438; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=2.98060202283; seq_b_insertion=0; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +ddbdddccddddddddddddddddddddaaddddddcddbcdddd~b}bb~b~~~b~q~~}~}~x~x~t~s~|k|~w~~z~~{|{~ccBc~~}~~~~~~a~~a~}p~~baceddbbedddda_aa_c[cdadddddddddddddddadddadd +@HELIUM_000100422_612GNAAXX:7:114:4511:17596#0/2_CONS ali_length=62; direction=left; seq_ab_match=57; sminR=40.0; seq_a_mismatch=4; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; seq_b_single=46; score=207.883730153; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.35296338956; seq_b_insertion=0; +ccgcctccttagaacaggctcctctagaagcgtataaagcaccgccaagtcctttgagttttaagctattgccggtagcactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg ++ +ddeedecddddddbdddcdeeddddPQPPPKKPQPPPPPQdddddd~~~~b~~~~~~~~~~~~~~~~~as~a~~~~~}W~~~~~~~~~~~~~X~~~~~~~~~~~~~~~d^d^dddddddddddddddddddddddbdddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:94:12367:17729#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; seq_a_mismatch=3; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=223.888596169; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.61110638983; seq_b_insertion=0; +ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg ++ +dddddddddddddddddddddddddddcceddccdadbd`dddddcb~~~b}~~~~~~~~~~~~~~~~~x||t~|r~~~~~~~~b~~~~~~~~~~~~~~~~~~~~~~~daddedddddddddd`dddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:30:5374:18213#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=45; score=251.958026467; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.99933375344; seq_b_insertion=0; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +dddddddddddddeddddddddddddddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:49:11740:5547#0/2_CONS ali_length=63; direction=left; seq_ab_match=62; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; seq_b_single=45; score=243.959827817; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.87237821931; seq_b_insertion=0; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +ddaddcdddddddedddddddddddddeddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~cddddddddcdddddddddddddddddddddedddddddddd`dd +@HELIUM_000100422_612GNAAXX:7:29:3531:8314#0/2_CONS ali_length=63; direction=left; seq_ab_match=61; sminR=40.0; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=45; score=235.837856208; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.74345803504; seq_b_insertion=0; +ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +dddddddddddddcddddddddddddddddddcddddddddddcd~a~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~}~~~~~~~~~~~~~~~~~~~~~ddceddddddddddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:114:10615:11217#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; seq_b_single=46; score=231.702876359; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.73714316708; seq_b_insertion=0; +ccgcctcctttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctaaggaggcgg ++ +ddcdddcddcd`d__^dddddd`cddddddddaaa``a_adddd^d~b~~z~~~~~~~~~~~~~cBcccccccccccccc~y~~s~~~~~~~|~~~~~|~~~~sz~~~adddddd[dad`VRW]VZdddddddddddddc``_ccddddddddd +@HELIUM_000100422_612GNAAXX:7:108:2729:8600#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; seq_b_single=46; score=223.856778095; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.61059319508; seq_b_insertion=0; +ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg ++ +dddddddddddddeddddddddcddddddddddWddddcddddddd~~~~b~~~~~}~~~~~~~~~~~`~~~~~~~~~~~~~~~~~~~~~~~a~~~~~~~~~~~~~~~dacdcdeddddddddddddddedddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:103:8847:6326#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=45; score=251.968264217; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.99949625741; seq_b_insertion=0; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +ddddddddddddddddddddddddddddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddcddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:70:11798:2668#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=239.988667111; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.8707849534; seq_b_insertion=0; +ccgaagtagttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacctttctagaggagcctgttctactacttcgg ++ +dddddddddddddddddddddddddddddddddddddddddddddd~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dcdddddcddddddddbddedddddddcdeddeddddddddddddd +@HELIUM_000100422_612GNAAXX:7:4:18261:9148#0/2_CONS ali_length=63; direction=left; seq_ab_match=61; sminR=40.0; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; seq_b_single=45; score=235.937993338; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.7450475133; seq_b_insertion=0; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +ddeddddddddddcdddddddddddddeddddddddddddddddd~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~`~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:29:18416:16620#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=231.873033258; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.7398876332; seq_b_insertion=0; +ccgcctccttagaacaggctcctctagcagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagaactctggcgaataattttgttataataattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg ++ +ddddddddddddddddccddddddd`d\dd`bH[`bbb^bdddddd~~~~~~~~~~~~~~~~~~~~~~~~~`~s~u||Fz~~~~~~~~~~~~~~~~~~~~~~~~~~~~d`ddcdbdcddbbbb\dadddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:32:18262:6966#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; seq_b_single=46; score=239.975704028; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.87057587142; seq_b_insertion=0; +ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg ++ +edddddddddddddddddddddddddddddddddcddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~`~~~~~~~~~~~~ddddddddddbdddddddddddddcdddddddbddcdcddcdddbb +@HELIUM_000100422_612GNAAXX:7:110:17877:11976#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; seq_b_single=46; score=223.833250149; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.61021371209; seq_b_insertion=0; +ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg ++ +ddddddddddddddddddddddddddd_ddddadddddddddddddb~~~b~~~~~~~~~~~~~~~~~~~vqx{w~~~~~~~~~~x~~~~~~~~~~~~~~~~~~~~`~b\d^dddddddddddcdddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:97:9416:9155#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=231.872797448; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.7398838298; seq_b_insertion=0; +ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg ++ +ddddddddddddddddddddddddddddddddeddddddddddaddb~~~~~~~~~~~~~~~b~~~~~~{~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~eddddddddddddddedddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:72:8905:10715#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=45; score=251.929473647; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.99888053408; seq_b_insertion=0; +ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +dddddddddddddeddddedddddddddddcdddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddcdddddadddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:48:5296:16375#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=45; score=251.994265764; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.99990898038; seq_b_insertion=0; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +dddddddddddddcdddd`ddddddddcddbdddddaddddbddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddd`dbdaaddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:53:6550:1163#0/2_CONS ali_length=63; direction=left; seq_ab_match=61; sminR=40.0; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=45; score=235.996766828; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.74598042584; seq_b_insertion=0; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgtttgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +dddddddddddddddddddddddedddddaddddddddddddddd~b~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddddddddddddddddddddeddddddddddddd +@HELIUM_000100422_612GNAAXX:7:120:1900:13295#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=1; seq_b_single=46; score=219.874676069; seq_a_insertion=1; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.54636574304; seq_b_insertion=0; +ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgcgcgtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg ++ +dddddddddddddcdddddddddddddddddddcdddddcdddddd~~~~b~~~~~~~~~~~~~~~~~~~opfFhtpp~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dcddddcdddcdddddcdddddddddddddddeddddedddddddd +@HELIUM_000100422_612GNAAXX:7:96:19317:7551#0/2_CONS ali_length=62; direction=left; seq_ab_match=58; sminR=40.0; seq_a_mismatch=3; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; seq_b_single=46; score=215.85731879; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.48156965791; seq_b_insertion=0; +ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg ++ +ccccccbccccccccccccccc^cccccccbcXcbccccccccccc~q~|a~~~~~~|~~~~az~~~|_snppphy~~xq{tzz~~~~~~~~Z~~~~~~~~~~~~~~~d_ddddddddcddd`bbdddddddcddddddddddddddddddddc +@HELIUM_000100422_612GNAAXX:7:93:18556:10479#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; seq_b_single=46; score=223.913795643; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.61151283295; seq_b_insertion=0; +ccgcctcctttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctaaggaggcgg ++ +ddddddddcddcddcddddddddddddddddddddddddddddddd~b~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~dddddddddddddbdddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:44:19605:15509#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; seq_a_mismatch=3; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=223.916231984; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.61155212878; seq_b_insertion=0; +ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg ++ +dddddddddddddddddddddddddbddddddcddcdcdacddcdd~r~~`~k`~~p~~~_~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~z}y~~~~~~~~~~~~~~a_d_dddddddddddcdadddddddcdddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:40:3610:8896#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; seq_b_single=46; score=239.967241748; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.87043938304; seq_b_insertion=0; +ccgaagtagttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctactacttcgg ++ +dddddddddddddddddddddddddddddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~~~dddddddddddddedddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:41:19763:3556#0/2_CONS ali_length=62; direction=left; seq_ab_match=62; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=247.825851895; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.99719115959; seq_b_insertion=0; +ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg ++ +ddddddddddddddddddddddddddddddddcddddddddddddd{~~~~~~~~~~~~~~~~~~~v~~~~~~~~jz{m{~~~~~~~~~~~~~~~~~~~~~~~~~~~~dacddddddddddddcddddddddddddddededddddddddddWd +@HELIUM_000100422_612GNAAXX:7:111:7660:7465#0/2_CONS ali_length=63; direction=left; seq_ab_match=58; sminR=40.0; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=3; seq_a_deletion=0; seq_b_single=45; score=211.768626837; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.36140677518; seq_b_insertion=0; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +dbdbddcdddddddddd^dd`dddddcddcdddddd`ddddddcd~~~~~~~~_~~~~~~~~~~~~~Ky~~~~~~c~Y}~~s~~`~x~}~}~{~Z|h~~~~~~~~~~~dYYdddaddaaddad^dddcddddddcdddddddadcdddd``dd +@HELIUM_000100422_612GNAAXX:7:76:10822:18722#0/2_CONS ali_length=63; direction=left; seq_ab_match=58; sminR=40.0; seq_a_mismatch=5; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=45; score=211.83390837; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.36244299; seq_b_insertion=0; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacacgaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +ddddddbcdbdddddddd_dddddddcacbdcddddTbdcddddd~`~~~z~~~~~~S~~~~~~b~~`~~~t~t~~}~~~~~~~~~~~T~~~|~~~~~~~~~~~~x~~ddddadddd_ddbdcbdddadddcdddddddbdddddddbddddd +@HELIUM_000100422_612GNAAXX:7:76:8491:8900#0/2_CONS ali_length=62; direction=left; seq_ab_match=57; sminR=40.0; seq_a_mismatch=4; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; seq_b_single=46; score=207.828655885; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.35207509492; seq_b_insertion=0; +ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgcaggtagcactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg ++ +dcddddddddddddadddddddadddcaddddadcdddddddddddb~~~b~~~~~o~~~~~~~~~~~~~z}F}~xq~N}~~y~~~}~~~~wu~~~~~~~~~~~~~^~b[bcc_ccccbcccccccbcbabacccccccbc_ccbbb`bccccc +@HELIUM_000100422_612GNAAXX:7:47:7619:9866#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=239.867362445; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.86882842653; seq_b_insertion=0; +ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg ++ +dddddeddddddedddddddddeddddddddddddddddddddddda~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dedddddddddddddddddddddddddddddddddddddddedddd +@HELIUM_000100422_612GNAAXX:7:1:8649:5297#0/2_CONS ali_length=104; direction=right; seq_ab_match=104; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=4; seq_b_mismatch=0; seq_a_deletion=4; seq_b_single=0; score=415.823014771; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=0; score_norm=3.99829821895; seq_b_insertion=0; +ccagcgagatcccattgagtctctgcacctatcctttttttcgttttctgaatctttgtttggaaaaaaacaggatttggctcaggattgcccgatctcgctgg ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@HELIUM_000100422_612GNAAXX:7:99:14742:5591#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=231.982488051; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.74165303308; seq_b_insertion=0; +ccgcctcctttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctaaggaggcgg ++ +dddddddddddddddddeddddddddddddddddddddddddddcd~b~~~~~~~~~~~~~a~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddddcddddddddddcdddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:95:3274:5435#0/2_CONS ali_length=63; direction=left; seq_ab_match=61; sminR=40.0; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; seq_b_single=45; score=235.944669323; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.74515348131; seq_b_insertion=0; +ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +dddddddddddcddddddddddccddddddddddcdddddddddd~~~~~b~~~~~~~~~~~~~~~~~~~~~~~~~{~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~ddddcdddddddddcdddddddddddedddddddeddcdcddddd +@HELIUM_000100422_612GNAAXX:7:74:8396:18135#0/2_CONS ali_length=63; direction=left; seq_ab_match=61; sminR=40.0; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=45; score=235.858013544; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.74377799276; seq_b_insertion=0; +ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +ddddddddddddddddddddddddddddddddddddddddddddd~W~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddcddddddddddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:9:10358:4089#0/2_CONS ali_length=61; direction=left; seq_ab_match=54; sminR=40.0; seq_a_mismatch=1; seq_b_deletion=1; seq_b_mismatch=6; seq_a_deletion=0; seq_b_single=47; score=181.620394786; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=2.97738352108; seq_b_insertion=0; +ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagttctttgagttttgagctattgcctgtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg ++ +ddcddddddddbdddddddddd`ddadddddda`]][\[]cdd`ccacccBcccccccccccBccccccccccBccccBcccccccccccccccc~~~ZzzzwX~yz`^dcddcddddddabdad^dddbbcbdddddddbdbbcddddddddd +@HELIUM_000100422_612GNAAXX:7:78:10338:12302#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=231.881878067; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.74003029141; seq_b_insertion=0; +ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg ++ +ddddcddddddddddddddddddddddddddddddddddddddddd~a~~~~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddeddddddddddddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:95:17941:6113#0/2_CONS ali_length=63; direction=left; seq_ab_match=57; sminR=40.0; seq_a_mismatch=6; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=45; score=203.869927289; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.23603059189; seq_b_insertion=0; +ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +ddedddddddddedddddddddddddddddddddddddddddddd~b~~~b~~b~~~bb~~~~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~acede^cdddc`dd`ddddedadddedddddddddddcdddaacc +@HELIUM_000100422_612GNAAXX:7:59:11971:12063#0/2_CONS ali_length=62; direction=left; seq_ab_match=62; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=247.995051728; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.99992018915; seq_b_insertion=0; +ccgcctcctttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgtcagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctaaggaggcgg ++ +ddddddddddddddddddddeddddddacddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~|~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddcdddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:47:3878:6141#0/2_CONS ali_length=62; direction=left; seq_ab_match=62; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=247.947034203; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.99914571295; seq_b_insertion=0; +ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg ++ +dddddddddddddddddddddddddddddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddddddddddddddddddddcddddddddddddd +@HELIUM_000100422_612GNAAXX:7:21:5487:19582#0/2_CONS ali_length=62; direction=left; seq_ab_match=58; sminR=40.0; seq_a_mismatch=3; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; seq_b_single=46; score=215.831528178; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.4811536803; seq_b_insertion=0; +ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg ++ +dddaddbddddcdcddddddddddddddbddcdddcddddddddd_~v}~~}{~~~~b~}ZZ}}}t~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~d`dbdddbdcdcdacddddddddddddaddddddddddeddddddd +@HELIUM_000100422_612GNAAXX:7:38:13721:2004#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=239.856433937; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.86865216027; seq_b_insertion=0; +ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg ++ +ddcccd`dddddddddddddddddddbbddbd^ddabddddddddd~{~~~~~~~~~~~~b~~~~~~~~~~~~~~~}}~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~d`ddddddddddddddd]addddbddddddddcddddddddddddd +@HELIUM_000100422_612GNAAXX:7:111:19168:18517#0/2_CONS ali_length=63; direction=left; seq_ab_match=60; sminR=40.0; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; seq_b_single=46; score=223.825469474; seq_a_insertion=1; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.55278522975; seq_b_insertion=0; +ccgaagtagttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattatccgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctactacttcgg ++ +ddddddddddcddddddddddddddcdddbddddddbcdddd^dd~~~{~_~~~~~~~~~y{}{~U~{|~~~~~n~~|~~~~}x~~~~~~~~~~~~~~~~~^~~~~~~ccdaddbdddbddb_ddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:89:16162:8889#0/2_CONS ali_length=63; direction=left; seq_ab_match=62; sminR=40.0; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=45; score=243.971622168; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.87256543123; seq_b_insertion=0; +ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattg ++ +dddddedddeddddddddddddddddddddddddddddddddddd~~c~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddedddddddeddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:74:4888:6953#0/2_CONS ali_length=63; direction=left; seq_ab_match=61; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=2; seq_a_deletion=0; seq_b_single=45; score=235.986171514; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.74581224625; seq_b_insertion=0; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +ddddddddddddddddddddddddddddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~]~~~~~~~~~~~~~~~~~~~dddddddddeddddddddddddddddddddcdddddddddddddd +@HELIUM_000100422_612GNAAXX:7:41:16707:19819#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=231.94731954; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.74108579903; seq_b_insertion=0; +ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg ++ +dedddddddddddbddedadddddccddddddaddbddd^a`daddb~~~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~bbdddddddddddddddddddddddddddddddccddddddddddd +@HELIUM_000100422_612GNAAXX:7:7:7916:16505#0/2_CONS ali_length=58; direction=right; seq_ab_match=58; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=50; seq_b_mismatch=0; seq_a_deletion=50; seq_b_single=0; score=231.999536374; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=0; score_norm=3.99999200644; seq_b_insertion=0; +ccgcctccttagaacaggctcctctagaaaagcatagtggggtatctaaaggaggcgg ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@HELIUM_000100422_612GNAAXX:7:41:9316:2256#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=239.993848494; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.87086852409; seq_b_insertion=0; +ccgcctcctttagataccccactatgcttagccctaaacacaagaaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctaaggaggcgg ++ +dddddddddddeddddcdddddddddddddddddddddddddddNd~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddcadedddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:67:7582:3642#0/2_CONS ali_length=63; direction=left; seq_ab_match=62; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; seq_b_single=45; score=243.962430842; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.87241953718; seq_b_insertion=0; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +ddddddddddddddddddddddddddddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~`~~~~~~~~ddddddddddddddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:42:8435:4155#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=45; score=251.987540492; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.99980223003; seq_b_insertion=0; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +ddddddddeddddddddddddddddddddddddddddd`dddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:1:8702:15835#0/2_CONS ali_length=85; direction=left; seq_ab_match=85; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=23; score=339.959438284; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=23; score_norm=3.99952280334; seq_b_insertion=0; +cccatcgctcagatatccgttgccgagagtcgtgtagattatatagaattgcaactcggggtgcggccagcaagccagccacatcccctcgttaggcacagtgttccttgacgccttcggtgagcgatggg ++ +ddddddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~{~~~~~~~~ddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:33:9900:9541#0/2_CONS ali_length=62; direction=left; seq_ab_match=62; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=247.949713348; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.99918892497; seq_b_insertion=0; +ccaattaacttagataccccactatgcctagccctaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg ++ +dddddddddddddcddddddddddddddddddd\`cccccdddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddedddddddddecddcddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:48:7139:3789#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=45; score=251.902451987; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.99845161885; seq_b_insertion=0; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +ddddddddddddddddddddddddddddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:46:13269:4134#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=45; score=251.997344433; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.99995784814; seq_b_insertion=0; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +dddddddddddddeddddddddddddddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddcdddddddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:46:9246:19171#0/2_CONS ali_length=62; direction=left; seq_ab_match=52; sminR=40.0; seq_a_mismatch=10; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=167.802718427; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=2.70649545851; seq_b_insertion=0; +ccgcctcctttagataccccactatgcttagccctaaacacaagtaattattataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctaaggaggcgg ++ +ddadddddddcaddddddddbdcdddd`dddddddddd^bddadcd~b~~~~~b~~~~~~~_`~~~~~b~~~~~S~~~~~~~~~~b~~n~`~~k~zoY|~~~~jb~~~DDDDDDDDDDDDDDDDDD`M\^ca__T^^Y^c^c_[`[ddddd`cd +@HELIUM_000100422_612GNAAXX:7:37:12979:6595#0/2_CONS ali_length=58; direction=right; seq_ab_match=58; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=50; seq_b_mismatch=0; seq_a_deletion=50; seq_b_single=0; score=231.998796685; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=0; score_norm=3.99997925319; seq_b_insertion=0; +ccgcctcctttagataccccactatgcttttctagaggagcctgttctaaggaggcgg ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@HELIUM_000100422_612GNAAXX:7:95:1688:9598#0/2_CONS ali_length=63; direction=left; seq_ab_match=54; sminR=40.0; seq_a_mismatch=6; seq_b_deletion=0; seq_b_mismatch=3; seq_a_deletion=0; seq_b_single=45; score=179.847313607; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=2.8547192636; seq_b_insertion=0; +ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtatcactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +aaaddbdbddbd`bdc^`[cddbddd`d_ddddcd^^`[`ddd[d~~~~pW~~`xzxwr~p{vvwxjwItwwrxxm|FPS~~}}}~z|~~__~~~~~~~~~~~~~[~~bbacb`adcdddd`cddddddddbbddddddddddddcdddcddd +@HELIUM_000100422_612GNAAXX:7:55:16528:11418#0/2_CONS ali_length=62; direction=left; seq_ab_match=58; sminR=40.0; seq_a_mismatch=4; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=215.988419267; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.48368418172; seq_b_insertion=0; +ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcactagcttacaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattag ++ +ddddbadddddddccddccddddaaadddcdddddddddddcd`da~~~~~~~~~~~~x~~~~~\~~~~~~~~~v~~~~~~~~~~\~~~~~~~[~~~s~~~~~~~~]~cY__]\QPPPP`]`[`c^^[cccccccccccaba_a\``]_WK[T[ +@HELIUM_000100422_612GNAAXX:7:32:18486:7018#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=45; score=251.909476689; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.99856312205; seq_b_insertion=0; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +dddddedddddddddddddddddddddddddddddddddddedcd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddeddddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:85:2105:21152#0/2_CONS ali_length=63; direction=left; seq_ab_match=57; sminR=40.0; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=4; seq_a_deletion=0; seq_b_single=45; score=203.706262238; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.23343273394; seq_b_insertion=0; +ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +ddddddddddddddddddddddddccdddddcddedddeddddbd~b~~{~~~~~}~}\~~~~cccccccccccccccccc~~~x~j~~~~~~~~~b~`s~b~~~~b~d`dddaddddddddddaddaddddcdddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:83:6795:14061#0/2_CONS ali_length=62; direction=left; seq_ab_match=62; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=247.992382324; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.99987713426; seq_b_insertion=0; +ccgcctcctttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctaaggaggcgg ++ +dddddddddddddcddddddcdcddddddddddddddddddddd_d~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddcddddddddddbdddbdddddddddcddddeddddddddddddd +@HELIUM_000100422_612GNAAXX:7:73:16525:10764#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=231.90336822; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.74037690678; seq_b_insertion=0; +ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg ++ +ddcddddddddddedddddddddddddadddd[bddddb_dddddd~~~~~~~~~~~~~~b~b~~~~~|~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b^ddddcddcddddddd_cdddddcddbdddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:19:6313:16567#0/2_CONS ali_length=63; direction=left; seq_ab_match=60; sminR=40.0; seq_a_mismatch=3; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=45; score=227.826165596; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.61628834279; seq_b_insertion=0; +ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagccctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +ddbcddddcdddbdcdcdddddadddcddcdYab`__b_bddcdd~~~~O_~~~~~}~~u}~~~~~~~a|~~~z{mrwvx~r~|w}}~~~~~~~~~~~~~~~~~~~~~dddddcaddddddcddddddeeddddddddebddddddddddddd +@HELIUM_000100422_612GNAAXX:7:114:8865:19510#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=239.913762365; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.86957681233; seq_b_insertion=0; +ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg ++ +ddddedddddddddddddddddcddddddddddddddddddddddd~a~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddcdddddddddddddddedddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:111:8478:1127#0/2_CONS ali_length=63; direction=left; seq_ab_match=57; sminR=40.0; seq_a_mismatch=5; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; seq_b_single=45; score=203.794981474; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.23484097578; seq_b_insertion=0; +ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgcgttccaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +dddd_ddddcdadddddcddddbdddddcd^dabdddcaddddda~]v~~Z~~`puIwxxH~|~}~~~~~~~|~~y}vp~{~~~~|yz~~~~~~~{~~~~~~|~~~b~ddbMddddddddddadddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:81:15726:2345#0/2_CONS ali_length=63; direction=left; seq_ab_match=56; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=7; seq_a_deletion=0; seq_b_single=45; score=195.641193686; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.10541577279; seq_b_insertion=0; +ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccgagtgctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +````Z]]cbb`ccbcc[`ca`bU[cQZ___cQbc`DDDDDDDDDDcBcccBccccccccccccccBccccccccccBcBccccccBccBc~~~~qp~wws~~{~~s~~b_cc__ba_accb_cXJWXWaabcac_c`[__[[_]_aa__`___ +@HELIUM_000100422_612GNAAXX:7:13:14386:20412#0/2_CONS ali_length=63; direction=left; seq_ab_match=60; sminR=40.0; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; seq_b_single=45; score=223.918527801; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.55426234604; seq_b_insertion=1; +ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg ++ +dddddddddddddddddddddddddddcdddddddddddddddddd~~~~b~~~~~~~~~~~~~~~~~~t~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ax~~~~a~~dddddddddddddddcedddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:83:18882:9967#0/2_CONS ali_length=63; direction=left; seq_ab_match=60; sminR=40.0; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; seq_b_single=45; score=227.922890965; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.6178236661; seq_b_insertion=0; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +ddddddddddddddddddcdddddddddddddddddddddddddd~b~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~~dddddddddddddddddadddddddddcddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:13:17353:13234#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=2; seq_a_deletion=0; seq_b_single=46; score=223.801902424; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.60970810361; seq_b_insertion=0; +ccgaagtagtagaacaggctcctctagaaaggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg ++ +ddddedddddddddddddddddddcdddddbcUcbdddbddbddadb~~~~~~~~~~~~~~~~~~~~~~~~~~~~~|~~~~~~~~~~~~~]pVo~~~~~x~~~~~}~~b`dWddeddddddddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:53:10153:11845#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=239.985596162; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.87073542196; seq_b_insertion=0; +ccgaagtagttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctactacttcgg ++ +dddddddddddddcddddddddcddddddddddddddddddddddd~~~~~~~~~~~~~~~a~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddedddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:33:9296:13526#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=239.852481933; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.86858841828; seq_b_insertion=0; +ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg ++ +ddddadddddddddddddddddcdddddcdddZdddddbddddddd^~~~~~~~~~~~~~~~~~~~~~~~v~~~~~|~|~~~~~~~~~~~~~~~~~~~~~~~~~~~~~d^ddddddddeedddddcdddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:113:2918:18736#0/2_CONS ali_length=62; direction=left; seq_ab_match=58; sminR=40.0; seq_a_mismatch=4; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=215.87931956; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.48192450903; seq_b_insertion=0; +ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg ++ +dddddddddddddddddddddddddddddddd_adddddcddddcdb~~~b~~b~~~~~~~~~~~y~~`y~~v~~~v~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~d`_ddddddddddddcdcdddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:63:4725:12502#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=239.998975242; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.87095121358; seq_b_insertion=0; +ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg ++ +ddadcdddcdddddddddcdddaddadTdd\]R\]ddd``dddddd~~~~~~~~~~~~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~d]dWddddddddeddcdcddddddddddddddddcddddddddddd +@HELIUM_000100422_612GNAAXX:7:7:11110:17924#0/2_CONS ali_length=62; direction=left; seq_ab_match=58; sminR=40.0; seq_a_mismatch=3; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; seq_b_single=46; score=215.812113201; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.4808405355; seq_b_insertion=0; +ccgaagtagttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtcataccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctactacttcgg ++ +ddddcddd_dddddddddddddddcdddcddddddddcdddddd`d~b~~~~~~~~~~~~~~~~~rm{kripoooEJpoolppp~~~~~~~~~~~~~v~~~~~`v~~~dddcdddddddbddddaddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:102:6364:4627#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=45; score=251.985885517; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.99977596059; seq_b_insertion=0; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +ddddddddddddddddddddddddddddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddcddddddddddcdddddddddddddddddcddddddddddddd +@HELIUM_000100422_612GNAAXX:7:96:8737:4973#0/2_CONS ali_length=58; direction=right; seq_ab_match=57; sminR=40.0; seq_a_mismatch=1; seq_b_deletion=50; seq_b_mismatch=0; seq_a_deletion=50; seq_b_single=0; score=223.980140585; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=0; score_norm=3.86172656181; seq_b_insertion=0; +ccgcctccttagaacaggctcctctagaaaagcatagtggggtatctaaaggaggcgg ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@HELIUM_000100422_612GNAAXX:7:89:9710:20652#0/2_CONS ali_length=63; direction=left; seq_ab_match=62; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; seq_b_single=45; score=243.910293117; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.87159195424; seq_b_insertion=0; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttatatccctctagaggagcctgttctagatattcgg ++ +ddddddddddddd`addddddddd`dddad]ccccddcdddcddd~~}~~~~~~~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~}ddadddddddddddddddddddddddadddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:71:17473:7401#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; seq_b_single=46; score=231.883938289; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.74006352079; seq_b_insertion=0; +ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattaccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg ++ +cccccc^ccccccccccccccccc_cccccabaac^ccccc_cc^c~~~~R~~~~s~~w~~~~~xg~~~~powyw~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~~dddbddbdccddddaddddbdddddddacdddcddddddddddddd +@HELIUM_000100422_612GNAAXX:7:28:9406:18226#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=231.816671723; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.73897857618; seq_b_insertion=0; +ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg ++ +ddddddddddddddddddddddddddddddddcddddddddddedd_~~~b~~~~~~~~~~~~~~x~~~~~~~~~~{~~~~~~~~~~~~~~|~~~~~~~~~~~~~~~~dcedcdcddddddddddddddddddddcddddeddddddddddddd +@HELIUM_000100422_612GNAAXX:7:15:16118:15111#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=231.917355899; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.7406025145; seq_b_insertion=0; +ccgaagtagttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctactacttcgg ++ +dddddddddddddddddddddddddddddddddddddddddd`d]d~~~c~~~~x~~~~~~a~~~w~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddd`dddedddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:58:18618:20959#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=2; seq_a_deletion=0; seq_b_single=46; score=223.882241304; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.611003892; seq_b_insertion=0; +ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg ++ +ddddeddddddddddddddddddddddddddd_ddddddcdddddd~~~}b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~|~~~~~z~~x~~~~~~~~~b~~~~~bddddddddddddddddcddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:46:11654:6037#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=239.828425953; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.8682004186; seq_b_insertion=0; +ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg ++ +ddddddddddbd`adddddddddddddddddddbcbbb^bcddcccb~~~~~~~~~~~~~~~~}~~~~~~~{~~~v~~z{}~~~~~~~~~~~~~~~~~~~~~~~~~~~dcdddddddddcdddedddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:2:6320:8068#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=45; score=251.910304163; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.99857625655; seq_b_insertion=0; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +ddedddddddddddddddddddddddddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddeddddbdddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:46:1592:11866#0/2_CONS ali_length=62; direction=left; seq_ab_match=62; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=247.993604064; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.99989683974; seq_b_insertion=0; +ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg ++ +ddcdddcddddbdddcdddd_ddaddddd_dddbcd\dddddd]dd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddcddcddd`dddcddcdddddddddddddcdd`dddddcdd`ddd +@HELIUM_000100422_612GNAAXX:7:119:2660:19129#0/2_CONS ali_length=63; direction=left; seq_ab_match=61; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=2; seq_a_deletion=0; seq_b_single=45; score=235.859804076; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.74380641391; seq_b_insertion=0; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +ddddcdddedddedddddddddddddddcddcddddddddcdddd~~~~~~~~~~~~~~~~z}~~~~~~~~~~~~~~~~~~~~~~~~~~~~k~~~~~~~~~~b~^~~~dddbddddddddddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:115:3625:10051#0/2_CONS ali_length=59; direction=right; seq_ab_match=59; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=49; seq_b_mismatch=0; seq_a_deletion=49; seq_b_single=0; score=235.999642017; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=0; score_norm=3.99999393249; seq_b_insertion=0; +ccaattaacttagataccccactatgccaatactagaggagcctgttctagttaattgg ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@HELIUM_000100422_612GNAAXX:7:100:17253:5513#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=231.761122002; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.73808261294; seq_b_insertion=0; +ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg ++ +dddcddcddccddbddddddddbdcdcdddbdddddcddddddddd~~~~|{|~~~~b~~~~~~~~~~s~~~~~x~~~~~~~rs~zqwxy~z~~|}~z~}|{^v~~~~c``Zc`cXWcc[WYYZccccccc_ccccccccWbcc`cc]cccccc +@HELIUM_000100422_612GNAAXX:7:82:6036:6921#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=2; seq_a_deletion=0; seq_b_single=46; score=231.901981419; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.74035453901; seq_b_insertion=0; +ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg ++ +dddddddddddddddddddddddddcd`dddbaddddcdccddddd~{~|~~~~~~~~~~~~~~~~~~~}~~~~~~~~]~~~~~~~~~~~~~~~~~~~~~~~b~~~~~dcddddddddeddddbcddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:120:4726:20808#0/2_CONS ali_length=62; direction=left; seq_ab_match=62; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=247.810697755; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.99694673798; seq_b_insertion=0; +ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg ++ +ddddecdcdddddcedddddddddddddbedddddcdddddddddb~~~~~~~~~~~~~~ty}y~~~~~~~~~~~~{~~~~~~~~~~~~~~~~~~~~~~~~~~~~~r~ddddcdddddcdd`dddddedddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:99:8183:13912#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; seq_b_single=46; score=231.903850642; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.74038468777; seq_b_insertion=0; +ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctcctagaggagcctgttctagttaattgg ++ +ddddddddddddceeddddddddddddddddddddddcddddddeb~Z~~~~~~~~~~~~~~~~~}~l~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~dadcdedddcdddddddd`ddddddddddddddddcddddeddddd +@HELIUM_000100422_612GNAAXX:7:10:4085:7492#0/2_CONS ali_length=63; direction=left; seq_ab_match=62; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; seq_b_single=45; score=243.95581367; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.87231450269; seq_b_insertion=0; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +ddddddddddddddddddddddddddddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~ddddddddddddddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:35:12723:18557#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=45; score=251.945522222; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.99913527337; seq_b_insertion=0; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +ddddddddddddddddd`ddddddddaadddcddddddddddddd~~}~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~cdddcdddddddbddddddcdddddddddddcddddddddddddd +@HELIUM_000100422_612GNAAXX:7:97:10495:17046#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; seq_b_single=46; score=239.938979134; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.86998353443; seq_b_insertion=0; +ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg ++ +ddedecdddddddbbdddddddddcddddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~a~~~~~~~~~~~~dddddddddddddddcddddddddddedddddcddddddddcdddd +@HELIUM_000100422_612GNAAXX:7:73:17068:16620#0/2_CONS ali_length=58; direction=right; seq_ab_match=57; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=50; seq_b_mismatch=1; seq_a_deletion=50; seq_b_single=0; score=223.962007947; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=0; score_norm=3.86141393012; seq_b_insertion=0; +ccgcctccttagaacaggctcctctagaaaagcatagtggggtatctaaaggaggcgg ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~|~ +@HELIUM_000100422_612GNAAXX:7:23:13304:5167#0/2_CONS ali_length=62; direction=left; seq_ab_match=62; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=247.997553566; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.99996054139; seq_b_insertion=0; +ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg ++ +dddddddddddddddddddddddddddddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddcdddddddddddddcdddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:54:2542:19180#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=239.868258533; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.86884287957; seq_b_insertion=0; +ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg ++ +ddddddddddddddddddddddddddddddddbddddcdccddddd~~~~b~~~~~~~~~~~~~~~~~|~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dadbdddddddddddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:1:8613:9165#0/2_CONS ali_length=101; direction=left; seq_ab_match=99; sminR=40.0; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=7; score=387.825112777; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=7; score_norm=3.83985260176; seq_b_insertion=0; +ccagagcgagtgggcaatcctgagccaaatctttatttttagaaaaacaaaagggcttcagaaagcaaaaataaacataaaggataggtgcagagactcaatggactcgctctgg ++ +ddddddd~~~~b~~~~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddd +@HELIUM_000100422_612GNAAXX:7:109:17917:15159#0/2_CONS ali_length=63; direction=left; seq_ab_match=61; sminR=40.0; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=45; score=235.857230348; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.74376556108; seq_b_insertion=0; +ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +dddedddddddddddddddddddddddddddcddddddddddddc~~~~~b~~a~~~~~~~~~~~~~~~~~~~~~}~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddcdddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:59:18371:8149#0/2_CONS ali_length=62; direction=left; seq_ab_match=62; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=247.998742319; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.99997971482; seq_b_insertion=0; +ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg ++ +ddddddddddddddddddddddddddddcddddddddddddddddc~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddedddddddddddddddddcdddddddddddddd +@HELIUM_000100422_612GNAAXX:7:1:8670:8750#0/2_CONS ali_length=90; direction=right; seq_ab_match=90; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=18; seq_b_mismatch=0; seq_a_deletion=18; seq_b_single=0; score=359.914979278; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=0; score_norm=3.99905532531; seq_b_insertion=0; +ccgaggcttactaggattagataccctattatttttgaatgttaatgtttgtttgcttgagtagtattagttatgttcttgaaagcctcg ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@HELIUM_000100422_612GNAAXX:7:86:11013:15234#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=239.990009212; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.8708066002; seq_b_insertion=0; +ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg ++ +dddddddddddddddddddddddddddddddddddddddddddddd~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:77:18518:10758#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; seq_b_single=46; score=223.70605163; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.60816212307; seq_b_insertion=0; +ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg ++ +aaa]`_ccca_a`ccccccccc]c_c_caccaccccccccccc_cc~~~~~~~~~~~~wzz{~~a~~~~y~_~~~~~~~~~~m~sygwqv~ccBccccccccccccc~bb^^bc`U\\`\]XPZ\__[\Z`ZX`accccc``c`Rb`Z`bbccc +@HELIUM_000100422_612GNAAXX:7:63:18851:7688#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; seq_a_mismatch=3; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=223.854412144; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.61055503459; seq_b_insertion=0; +ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg ++ +ddddddddddddddddddedddddddddddddddeddddddddddd~~~~b~~~~~~~~~b~a~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddeddddddddddcdddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:22:1798:3790#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; seq_a_mismatch=3; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=223.82253574; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.61004089903; seq_b_insertion=0; +ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagcactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg ++ +ddddddddddddcdddddddddddddddddddadddddddddddddb~~~b~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddbddbdadddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:115:14354:10118#0/2_CONS ali_length=63; direction=left; seq_ab_match=59; sminR=40.0; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=2; seq_a_deletion=0; seq_b_single=45; score=219.850378027; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.4896885401; seq_b_insertion=0; +ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgcgagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +ddddddddddddddddddddddcdddddddddccddcdddddddd~~~~~b~~~~~~~~~~~~~}~~~~~~}Jt~y|w~~~~~~~~~~~~~~~_~~~~~~~~~~~~b~dcddddeddddddddeddcdddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:99:14794:3606#0/2_CONS ali_length=63; direction=left; seq_ab_match=61; sminR=40.0; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; seq_b_single=45; score=235.971679218; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.74558220981; seq_b_insertion=0; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +dddcdddddddcdddddddcddd``addddddddddddddddddd~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~dddddddddddddddddddddddddddddddcddddddddddddd +@HELIUM_000100422_612GNAAXX:7:74:8297:20108#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=239.962155962; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.87035735423; seq_b_insertion=0; +ccgaagtagttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctactacttcgg ++ +dddddddddddddddddddddddddddddcdddddddddddddd`d~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~}~dddddddddddddddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:29:12308:10119#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=45; score=251.866768717; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.99788521773; seq_b_insertion=0; +ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +ddddddddddddddddddddddddddddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddcdeddddedddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:25:11691:21134#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; seq_b_single=46; score=223.86269299; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.61068859661; seq_b_insertion=0; +ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg ++ +dddddddddddddddddddddd`ddcddddddadcdddaddddddd_~~~b~~}~~~~~~~~~~~~~~~}~}~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~d]daddddddcdddddcdddaddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:111:15484:19248#0/2_CONS ali_length=63; direction=left; seq_ab_match=60; sminR=40.0; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; seq_b_single=45; score=227.828264164; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.6163216534; seq_b_insertion=0; +ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +dddddddddddddddddddddddddddddddcdd`dddddddddd~~~~~b~~~~~~~~s~~~~~~~~`{~~}~xy}~{~~~z~{txvzj~~`~~~~~~~~~~~~~~~dddaddddddddddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:103:3594:15996#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=231.92194325; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.74067650404; seq_b_insertion=0; +ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacacaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg ++ +dddddedddddddddddddddddcddddddddddddddddddddddb~~~~~~~~~~~R~}~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddaddddddddddddd\bddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:1:14576:14181#0/2_CONS ali_length=63; direction=left; seq_ab_match=60; sminR=40.0; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=223.962708229; seq_a_insertion=1; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.55496362269; seq_b_insertion=0; +ccgaagtagttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctactacttcgg ++ +dddddddddddddddddddddddddddddddddddcddddddcda~~~~b~~~~~~~~~~~a~~~~~~`~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddedddddcdddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:99:13467:17714#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=2; seq_a_deletion=0; seq_b_single=46; score=223.932198733; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.61180965699; seq_b_insertion=0; +ccgcctcctttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctaaggaggcgg ++ +ddddcdddddddddcddddddddddddddcddddddddddddddcd~~~~~~~~b~~~}~~~~~~~~~~~~~~~~~~~~~~~^~~~~~~~~~~~~~~~~~~~~~~~~bddddddddddeddcdddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:43:7980:7908#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; seq_b_single=46; score=239.984831014; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.87072308087; seq_b_insertion=0; +ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg ++ +ddddcdddddddddddd`ddddddddbdaddcdddd_cddcdddda~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~`~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~d`ddddddddddccbdddddddddddddcdddeddddddddddddd +@HELIUM_000100422_612GNAAXX:7:105:14135:6905#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=239.985379811; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.87073193244; seq_b_insertion=0; +ccgcctcctttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcaccagagtactagcggcaacagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctaaggaggcgg ++ +ddddddddddcddedddddddddddddddddddddddddddddddd~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddeddddddddddddbdddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:89:16663:15081#0/2_CONS ali_length=62; direction=left; seq_ab_match=62; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=247.939062327; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.9990171343; seq_b_insertion=0; +ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg ++ +dddedddddddddddddddddddddddddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddecddddddddddddcdddddcddddddddddddd +@HELIUM_000100422_612GNAAXX:7:86:15907:21191#0/2_CONS ali_length=63; direction=left; seq_ab_match=58; sminR=40.0; seq_a_mismatch=3; seq_b_deletion=0; seq_b_mismatch=2; seq_a_deletion=0; seq_b_single=45; score=211.91203832; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.36368314793; seq_b_insertion=0; +ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +dddcdddddddddeddddddddaddddbdddcdddddadd\dcdd~b~~~a{~`~~~~~~~~~~~~~~~~~~~[~~~X~~~~~~~~~~~~~~~~~~~~~~~~{~~~~~ddddddddddddded`ddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:81:17261:2872#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=45; score=251.967130674; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.99947826467; seq_b_insertion=0; +ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +dddcddddddddcddddddddddddddddddddeddedddddadd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddedddddddddedddddddddddddddddddddddddddded +@HELIUM_000100422_612GNAAXX:7:14:15397:17819#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; seq_b_single=46; score=223.847163266; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.61043811719; seq_b_insertion=0; +ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctgttgccgctagtactctggtgaacaattttgtttgtgtaattatttgtgtttagggctaagcatagtggggtatctaaaggaggcgg ++ +ddcddddddddddddddddddddddbddddddcddddddbcddddd~~~~~~~a~~}~~~~~~~~v~~~s~a~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~`~cddddddddddddddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:76:18268:14980#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=239.985254914; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.87072991797; seq_b_insertion=0; +ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttaggggtaagcatagtggggtatctaaaggaggcgg ++ +ddddddddddddddddddddddbdcddbdbddHddcccb^dbdbda~~~~b~~~~~~~~~~~~~~~~~~~~z~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dceddddddddddcdcc\dddddddddddddedddddddddccded +@HELIUM_000100422_612GNAAXX:7:16:15509:18069#0/2_CONS ali_length=62; direction=left; seq_ab_match=58; sminR=40.0; seq_a_mismatch=4; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=215.81634366; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.48090876871; seq_b_insertion=0; +ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg ++ +dddddddddddddddddcdddd_cdcddbddaLdddddbddddddd_~}}b~~~~~~~~~~~~~}~~~~zz]~~~~~~~~~~~~~~r~a}~~~~~~~~~~~~~~~~~~adddcdbdddbdddddYdcdddcdddddddddddddcddcdddddd +@HELIUM_000100422_612GNAAXX:7:25:7789:3090#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=3; seq_a_deletion=0; seq_b_single=46; score=223.705772044; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.60815761361; seq_b_insertion=0; +ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgcctgtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg ++ +ddccdcddbdddddddddcdbbddc^T`_`]\L\Zccc^Tb\^abNt|rzu~~~||j|w~~~p~y~}z~~cccBccccccccccccBBc~~~~}~{~~~~~~~~~~~~dbddddddddddddd]daedddddddddddddeddddddddddddd +@HELIUM_000100422_612GNAAXX:7:53:18419:8404#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; seq_b_single=46; score=231.841887233; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.73938527796; seq_b_insertion=0; +ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg ++ +dddddddddddddedddddddddddddddddd_ddddddedddddd~~~~~~~~~{~~~~~~~~~~~~~~~b~~~~{~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~bddddddddddddddddcedddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:19:11039:3957#0/2_CONS ali_length=62; direction=left; seq_ab_match=62; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=247.811165598; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.99695428384; seq_b_insertion=0; +ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg ++ +dddddddddaddddddddddddddcccdddddbdddddabdddbdd~~~~~~~~~~~~~~~~~~~~~~~v~~~~~~~~~~}~~~~~~~~~~~~~~~~~~~~~~~~~~~dadddddddddddddcdddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:42:19757:3800#0/2_CONS ali_length=62; direction=left; seq_ab_match=57; sminR=40.0; seq_a_mismatch=1; seq_b_deletion=1; seq_b_mismatch=3; seq_a_deletion=1; seq_b_single=46; score=187.78301321; seq_a_insertion=1; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.02875827758; seq_b_insertion=0; +ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgtatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg ++ +dddeddddddddddddddddddddddddcdddaddddd`dddddd~~~~~~]~~~~~~~~~~~~~_x~~~~~~~~~~~zv~~~~~~~~~~~`~a~~~|~b~~~~~~~ccdcddddddedddeddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:116:14244:17926#0/2_CONS ali_length=63; direction=left; seq_ab_match=55; sminR=40.0; seq_a_mismatch=5; seq_b_deletion=0; seq_b_mismatch=3; seq_a_deletion=0; seq_b_single=45; score=187.882758912; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=2.98226601448; seq_b_insertion=0; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgcaagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +dddddddddddddddddddddddedddd`dbcccc`dddddeddd~~~~~~_~~~~b~~~~~~^xz{ZRzpooop~~s~~~~~~`~~~~~~~~~~~~~~~~~b~~~b~dddddeddddddddddddddeddddadddddeddddddddddddd +@HELIUM_000100422_612GNAAXX:7:91:10071:8960#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=45; score=251.979171474; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.99966938848; seq_b_insertion=0; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +dddddddddddddddddddddddddddddddddddddddddeddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:61:17484:5085#0/2_CONS ali_length=62; direction=left; seq_ab_match=62; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=247.996979391; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.9999512805; seq_b_insertion=0; +ccgcctcctttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctaaggaggcgg ++ +ddcdddddddddddddddeddddddddddddedddddddddddd`d~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddccddcdddddddddddddddddbddddddddedcd +@HELIUM_000100422_612GNAAXX:7:18:2721:16829#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=45; score=251.960802952; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.99937782464; seq_b_insertion=0; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +ddddecdededdedddddddddddddddddeeddddedddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dbddddddddddddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:2:7296:17275#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=2; seq_a_deletion=0; seq_b_single=46; score=223.923525795; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.61166977089; seq_b_insertion=0; +ccgcctcctttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctaaggaggcgg ++ +ddcddcdd_dddddddddddddcdddddbcacbcbd`ddddddd_b~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~vbdddddddddddcc]baddbddddbdddddddddddddcdddddddd +@HELIUM_000100422_612GNAAXX:7:58:8591:17028#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; seq_b_single=46; score=223.788176649; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.60948672015; seq_b_insertion=0; +ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg ++ +cc^cccccacabcaaccccccccc_\_[__ccaccccc`ac]caaa~~~~_~~`~wx~z~~~~~xxuz{~~~~~~ppppkzzrz~~x~~~~nyw~~~~~y~~a~~~~~ccccccaccbcccccccb_cccccccc_ccccc^]ccccccccccc +@HELIUM_000100422_612GNAAXX:7:13:3469:18630#0/2_CONS ali_length=63; direction=left; seq_ab_match=58; sminR=40.0; seq_a_mismatch=3; seq_b_deletion=0; seq_b_mismatch=2; seq_a_deletion=0; seq_b_single=45; score=211.840326012; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.36254485733; seq_b_insertion=0; +ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +dddddd`ddadddddddcddddcdddd`ddc_a`^dddd_ddddd~b~~~b~~[~~~~~~~~~~~~~~{yy|{xzsz~~~~~~~~~~~~~~~~~~w{VqLyyx~~~~~ddadcdddadPPQQIdddddddddddddddcddddddd_ddcd`d +@HELIUM_000100422_612GNAAXX:7:72:12959:5736#0/2_CONS ali_length=63; direction=left; seq_ab_match=56; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=7; seq_a_deletion=0; seq_b_single=45; score=195.664863172; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.10579147892; seq_b_insertion=0; +ccgaatatctagaacaggctcctctagaggggtgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +__T__WY_b_Sb__X[_\S_bDDDDDDDDDDDDDDDDDDDDDDDDccccccccccccccccccccBcccccccccBBBcc~_~~~~~~bi~~b~~~~~~~~~~~~~~~dddd^dddddbbbbSb\^b^cacdddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:115:6263:17700#0/2_CONS ali_length=63; direction=left; seq_ab_match=61; sminR=40.0; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=45; score=235.853550005; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.74370714293; seq_b_insertion=0; +ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +ddddddddddddddddddddddddddddddddddddddddedddd~b~~~b~~~~~~~~~~~~~~~~~~~~~{~~xxy{z|y~~~~~~~~~~~~~~~~~~~~~~~~~~dddedddddddddddcdddddddddddddddcddddddddddddd +@HELIUM_000100422_612GNAAXX:7:7:15538:8325#0/2_CONS ali_length=63; direction=left; seq_ab_match=52; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=1; seq_b_mismatch=10; seq_a_deletion=0; seq_b_single=45; score=151.662770749; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=2.40734556744; seq_b_insertion=1; +ccgaatatctagaacaggctcctctagagggatgtaaagcaccgcccagttctttgagtttcatgctgttgctagtagtactctggccgacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +__\b\][\XVbZX_\_b[abbDDDDDDDDDDDDDDDDDDDDDDDDcBcccBcccccBccccccBccccccBBccccccccccccccBcca~~~^YgzYxq~~~~}y~~_ccccac_accacba\S_][_cc^cccaccbacba`a_c_`a_aa +@HELIUM_000100422_612GNAAXX:7:120:16005:7955#0/2_CONS ali_length=64; direction=left; seq_ab_match=61; sminR=40.0; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=2; seq_a_deletion=0; seq_b_single=44; score=231.880428082; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=44; score_norm=3.62313168878; seq_b_insertion=0; +ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +dddddddddddddddddddddddddddddddddddddddcdddd~~~~~~~~~~~~~~~~~~`~~~~~~~{~~~~~~~~~~z~~~~~~~~~~~b~~~~~~~~~b~~~~dddddddddddddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:115:3396:14235#0/2_CONS ali_length=63; direction=left; seq_ab_match=61; sminR=40.0; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; seq_b_single=45; score=235.745732131; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.74199574811; seq_b_insertion=0; +ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +ddddddddddddddddddddddddddddddddcdcdddddddddd~~~~~b~~~~~~~~~~~~~~~~~~~s~~~~~~~{~~~~|~ccc~}~}~~~~~~~~~b~~~~~~dddcdddddadddddcdddddddddddddddeddddddddddddd +@HELIUM_000100422_612GNAAXX:7:32:15197:9940#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=231.86950825; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.73983077822; seq_b_insertion=0; +ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg ++ +dddddedddddddddddddddddddeddddddcddddddcdddd`db~~~~~~b~~~~~~~~~~~~~~~{~~~~~}}tw~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddcddddddddddddacddddddddddddcddddddddddddddcd +@HELIUM_000100422_612GNAAXX:7:7:17828:13825#0/2_CONS ali_length=62; direction=left; seq_ab_match=56; sminR=40.0; seq_a_mismatch=2; seq_b_deletion=1; seq_b_mismatch=3; seq_a_deletion=0; seq_b_single=46; score=187.930205125; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.03113234072; seq_b_insertion=1; +ccgcctcctttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctaaggaggcgg ++ +ddeddcdddddcddddddddddddddddddbddddddddacbaa[b~b~~~~~~~~~~~~~a~~~~~~~~~~~~b~~~~~~~~~~~b~~~~~~~b}~~~~~~~~~~~_ddddddddddddccdddcdddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:53:2128:13141#0/2_CONS ali_length=62; direction=left; seq_ab_match=62; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=247.978630411; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.99965532921; seq_b_insertion=0; +ccgaagtagttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctactacttcgg ++ +ddcddddddddddddddddddddddddddddddddddddddd^dad~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddcddddddddddcdddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:1:8644:15229#0/2_CONS ali_length=106; direction=left; seq_ab_match=103; sminR=40.0; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=2; seq_a_deletion=0; seq_b_single=2; score=399.617128202; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=2; score_norm=3.76997290756; seq_b_insertion=0; +cacacgctctccattgagtctctgcacctatcctttttgattttcgctttctgaatctttgtttgttttcggaaaacgtgatttggctcaggattgcccagagcgtgtgg ++ +dd~~~~~~~~~~~~~~~~~~~~~~~~{~~~~b~~~~~~~~~{|~~~~s|~v~~|p~~~~~~~~~~~u~qy~a~~~~~~~x~|~~~~z{~|~o|~{yvwwy~~|~~[~~cb +@HELIUM_000100422_612GNAAXX:7:72:7057:20448#0/2_CONS ali_length=62; direction=left; seq_ab_match=62; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=247.94833093; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.9991666279; seq_b_insertion=0; +ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg ++ +ddddddddddddddddddddddddddddddeddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddebddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:10:9237:10532#0/2_CONS ali_length=62; direction=left; seq_ab_match=57; sminR=40.0; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=3; seq_a_deletion=0; seq_b_single=46; score=207.90944986; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.35337822355; seq_b_insertion=0; +ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaattattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg ++ +ddcdccddddcddcdcdcdddd[a^[]]V]adadddacdddadddd~~~~b~~b~~~~~~o|~|xpppoj~~Y~~~~~~~~~~~~~~~\~~N~~~~~~~~~~~~~~~~`\_addddddddddd`cSccadddddd`dddddbabdcdddddddd +@HELIUM_000100422_612GNAAXX:7:16:16055:12835#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=239.868244499; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.86884265322; seq_b_insertion=0; +ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg ++ +ddddddddddddddddddddddddddddddddcddddddddddddd~~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~cdddddddddddcddd`bdddddcdddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:17:16877:9760#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; seq_a_mismatch=3; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=223.899159093; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.61127675956; seq_b_insertion=0; +ccgcctcctttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctaaggaggcgg ++ +ddddddddddddddddddddeddddcddddddddddddcddddddd~b~~~~~~~~~~~~~a~~~~~~b~~~{~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddddddddddddddddddddeddddddddddddd +@HELIUM_000100422_612GNAAXX:7:47:8320:8571#0/2_CONS ali_length=63; direction=left; seq_ab_match=61; sminR=40.0; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; seq_b_single=45; score=235.809300513; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.74300477004; seq_b_insertion=0; +ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +dddddddddcdddddddddddd`dd`daddd\ddddddaddddad~b~~~~~~~~~~x~~~~~|~~~~~~~~~~~yuu~~~~~~~~~~~~~~~~~~~~~b~~~~~|~~ddddddddddddddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:6:6617:2744#0/2_CONS ali_length=63; direction=left; seq_ab_match=61; sminR=40.0; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=45; score=235.875132744; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.7440497261; seq_b_insertion=0; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +ddddddddddddcadddddddddddddeddddd`ddddddddcbd~b~~~~b~~~~~~~~~~~~|~~~~~~~~y~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddcbddddeddddddddddeddddddddddddd +@HELIUM_000100422_612GNAAXX:7:12:9609:14740#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=231.833538451; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.73925062018; seq_b_insertion=0; +ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg ++ +dddddddeddddddddddddddddddddddddedddddcdddddddb~~~b~~~~~~~~~~~~~~~~~~}~~~~~}~sz~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddcddddddddddcdddddddddddddddddddddddedddd +@HELIUM_000100422_612GNAAXX:7:21:2093:4412#0/2_CONS ali_length=63; direction=left; seq_ab_match=60; sminR=40.0; seq_a_mismatch=3; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=45; score=227.763131817; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.61528780662; seq_b_insertion=0; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +dddddddddddddddddddddddddddeddddddddddddddddd~b~~~~~~~~~~~~~}~~`~~~~~~~~~~~~~Z{~~|{~~~~{~|~|~~~~~}~~~}|~~}}}DDDDDDDD``U`^\[dddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:18:5240:1587#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=2; seq_a_deletion=0; seq_b_single=46; score=223.905608015; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.61138077443; seq_b_insertion=0; +ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg ++ +dddddddddddddddddddddddddddddddddddddddddddddd~}~~~~~~~~~~~~}~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~bdddddddedddddcdddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:88:15797:19191#0/2_CONS ali_length=62; direction=left; seq_ab_match=58; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=4; seq_a_deletion=0; seq_b_single=46; score=215.88300628; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.48198397226; seq_b_insertion=0; +ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg ++ +ddddddddddddddddddddddddddddddddddddddddddcddc~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~`~~~~b~~b~~~~ddeddddddddddcdddddcddddddddcddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:1:8613:5665#0/2_CONS ali_length=90; direction=right; seq_ab_match=89; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=18; seq_b_mismatch=1; seq_a_deletion=18; seq_b_single=0; score=351.879189487; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=0; score_norm=3.90976877208; seq_b_insertion=0; +ccttcctggggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaccaggaagg ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~ +@HELIUM_000100422_612GNAAXX:7:47:11864:1343#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=45; score=251.976310174; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.99962397102; seq_b_insertion=0; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +dddddddddddddddddddddddddddddddddddddddddbdcd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddcdddddddddddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:43:8022:8427#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=231.869859511; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.73983644372; seq_b_insertion=0; +ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg ++ +ddddddddddddddddddddddddddddddddcddddddddddddd~~~~b~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddddcdedddddcdddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:95:11283:2557#0/2_CONS ali_length=58; direction=right; seq_ab_match=57; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=50; seq_b_mismatch=1; seq_a_deletion=50; seq_b_single=0; score=223.999078974; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=0; score_norm=3.86205308576; seq_b_insertion=0; +ccgcctccttagaacaggctcctctagaaaagcatagtggggtatctaaaggaggcgg ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~a~~~~~~~~~~~~~~~ +@HELIUM_000100422_612GNAAXX:7:67:5288:13403#0/2_CONS ali_length=62; direction=left; seq_ab_match=62; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=247.999078967; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.99998514464; seq_b_insertion=0; +ccgcctcctttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctaaggaggcgg ++ +ddeddddddddddddddddddddddddddddddddddcdddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddedddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:7:11998:4462#0/2_CONS ali_length=63; direction=left; seq_ab_match=59; sminR=40.0; seq_a_mismatch=4; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=45; score=219.864332815; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.48991004469; seq_b_insertion=0; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtagtactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +dddddddddddddddddddddddddddddddddddddddddcddd~b~~~~~~~~~~~~b~~~~~~~b~~~~~~~G~}}~~}}~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddcdccddedddddddcdddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:116:12598:9964#0/2_CONS ali_length=62; direction=left; seq_ab_match=53; sminR=40.0; seq_a_mismatch=8; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; seq_b_single=46; score=175.843125508; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=2.83617944368; seq_b_insertion=0; +ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg ++ +dddddddddddddddddddddddddddddddddddddddeddddddb~~~b~~~~~~~~~~~b~~~~~~{~b~b~~~~~b~~~~b~~~b~~~~~~~~~~~~~~~~~~`adddbdddddddcddddddddccdcedddeddddddddddbcecdd +@HELIUM_000100422_612GNAAXX:7:111:18277:17779#0/2_CONS ali_length=63; direction=left; seq_ab_match=61; sminR=40.0; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=45; score=235.877819976; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.74409238057; seq_b_insertion=0; +ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctattgctagtagtcctctggcgaattattttgttgtaaaattatctatgtttagggctaagcatagtggggtatctaagatattcgg ++ +dddddddddddddcdddddddddddddddddddddddeddddddd~~~~~b~~~~~~~~~~~~_~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dcdddbddddddddddddeddeddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:85:13257:14594#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=231.92296471; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.7406929792; seq_b_insertion=0; +ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctgttgccgctagtactctggtgaacaattttgtttgtgtaattatttgtgtttagggctaagcatagtggggtatctaaaggaggcgg ++ +dddddddddddeddddddddddddddddddddacdddddaddccdda~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~_~~~~~~~~~~~~~~~~~~~~~~~ddddddddddeddddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:17:11935:17343#0/2_CONS ali_length=63; direction=left; seq_ab_match=62; sminR=40.0; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=45; score=243.983570342; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.87275508479; seq_b_insertion=0; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +ddddddddddddddddddddddddddddddddddddddddddddd~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddddddcddddddddddddcddddddddddddd +@HELIUM_000100422_612GNAAXX:7:38:9654:18700#0/2_CONS ali_length=63; direction=left; seq_ab_match=62; sminR=40.0; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=45; score=243.950053136; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.87222306566; seq_b_insertion=0; +ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +dddddddddddddddddddddddddddcddddcddddddddddcd~~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddeddddddddddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:101:11720:19906#0/2_CONS ali_length=62; direction=left; seq_ab_match=57; sminR=40.0; seq_a_mismatch=4; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; seq_b_single=46; score=207.841888471; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.35228852373; seq_b_insertion=0; +ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg ++ +dddddddddddddddddddddddddddddddd^ddbbcc^dcdddd_~~~c~~b~~~~x~}~~~~}~~b~}sq~~}t{z~~{~~psh~|~~]~~~~~~~~~~~~~~~~d\ddddddcdddddd_cddddddddddddddddddddddddddcdd +@HELIUM_000100422_612GNAAXX:7:42:12961:8650#0/2_CONS ali_length=63; direction=left; seq_ab_match=62; sminR=40.0; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=45; score=243.985409859; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.87278428347; seq_b_insertion=0; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +ddddddddddddddddddddddddddddddddddddddddddddd~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddcddddddddddddddddddddddddddddeddddddddddddd +@HELIUM_000100422_612GNAAXX:7:36:10388:10324#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=239.950587748; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.87017077012; seq_b_insertion=0; +ccgaagtagttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctactacttcgg ++ +dddddddddddddddddddddddddddddddddddddddddddddd~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddeddddddddddddddddddcddddddddddddd +@HELIUM_000100422_612GNAAXX:7:66:11860:20419#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=239.911387587; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.86953850946; seq_b_insertion=0; +ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg ++ +ddeddddddddddddedd`dddddd`edddddNddddddddddcdd|~~~b~~~~~~~~~~~~~~~}~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~daddddddddedddddcddddedddddddddddddddddddddedd +@HELIUM_000100422_612GNAAXX:7:3:17077:6562#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; seq_a_mismatch=3; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=223.876050008; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.61090403239; seq_b_insertion=0; +ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggcagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg ++ +ccccccccccccccccccccccccbccccccccacccccccccccc~~~ua~~~~~~~~~~~~~~}~~~~~~~a~Fz~zz~~~~~~~~~~~~~~~~~~~~~~~~~~~~badadddddddddddcdddddddcddddddddeddddddbdddbdd +@HELIUM_000100422_612GNAAXX:7:18:11865:6418#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=45; score=251.959188225; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.99935219405; seq_b_insertion=0; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +ddddddddddddddddddddddddddddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddcdddedddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:19:4311:13343#0/2_CONS ali_length=63; direction=left; seq_ab_match=58; sminR=40.0; seq_a_mismatch=4; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; seq_b_single=45; score=211.837822462; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.36250511845; seq_b_insertion=0; +ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctcgtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +dddddddddddcd`ddcddddddcd\bcccdddddddddcdddcb~b~~~b~~~~~|~~~}~~~{y~~_~~~{Z{~~~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~ddddd^ddcddddcddbdddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:30:1800:7833#0/2_CONS ali_length=62; direction=left; seq_ab_match=52; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=10; seq_a_deletion=0; seq_b_single=46; score=167.712615339; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=2.70504218289; seq_b_insertion=0; +ccgaagtagttagataccccactatgcttagccctaaacacaagacattaatataacgagattaatcgacagagtactaccggctatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctactacttcgg ++ +DDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDccBccccccccBcBccccBBccBcccccccc~u~v}[wJ~~Q|~~~~~~~~~~~~a~~~~t~Z^ccc`c]bbc_[V_[`[````bb_cc[_cc[\[\[bcbbbccccc +@HELIUM_000100422_612GNAAXX:7:16:19299:8684#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=231.845804746; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.73944846365; seq_b_insertion=0; +ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg ++ +ddddddddddddddddddddddcdddddddddcddddddddddddd~~~~b~~~~~~~`~~~~~~~~~~~~~~~~~~~~~~~~|~~~~~~~~~~~~~~~~~~~~~~~~cadaddddddeddddccdcddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:54:14618:14353#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=239.8562981; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.86864996935; seq_b_insertion=0; +ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg ++ +ddddddddddddddddddcdddddddddddddcddddddcdddddd~~~~b~~~~~~~~~~~~~~~~~~y~~~~~z|}z~~~~~~~~~~~~~~~~~~~~~~~~~~~~~eddddddddddddddecddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:108:9222:18258#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=2; seq_a_deletion=0; seq_b_single=46; score=231.857703869; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.73964038498; seq_b_insertion=0; +ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcagacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg ++ +ddddeeddddddeddddddddddddddddccddddddddddddddd~~~~~~~~~~~~~~~~~~~v~~~~~}~}}~~~~~`~~~~~~~~~~b~~~~~~~~~~~~~~~~dcdddddddddddddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:74:4783:20479#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; seq_b_single=46; score=239.87314657; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.86892171887; seq_b_insertion=0; +ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg ++ +dddddddddddddddddd`dddcddcdadcddWdddddadWddbbcqy~~~~~~~~~~r~}~~~qx~~~~~z|~~~~~~~~~~~~~~~}~~~~~~~~~~~~~~~~~b~d`dZddddbddddddaddddddcddddbdddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:38:3005:20881#0/2_CONS ali_length=63; direction=left; seq_ab_match=60; sminR=40.0; seq_a_mismatch=3; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=45; score=227.901935995; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.61749104754; seq_b_insertion=0; +ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgttcatgtttagggctaagcatagtggggtatctaagatattcgg ++ +dddddddddddddddddddddddddddddddddbdddddcdddbd~~}~~b~~b~~~~~~|~~~~~~~a~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddeadddddddddddddddddddcddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:4:10139:2389#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=45; score=251.802206044; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.99686041339; seq_b_insertion=0; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +ddddddcddddddddddddd`dcdddddaddaddddbdddddddc~~~~~~~~~{~~~~~~~~zxy~~|~~~~ru~~~~~~~~~~~~~~~~~~{~~p~~~~~~~~~~~ddddddddddabaP`ddddabddddddddddddddddcdcdaddd +@HELIUM_000100422_612GNAAXX:7:75:9674:16966#0/2_CONS ali_length=58; direction=right; seq_ab_match=57; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=50; seq_b_mismatch=1; seq_a_deletion=50; seq_b_single=0; score=224.000075421; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=0; score_norm=3.86207026589; seq_b_insertion=0; +ccgcctccttagaacaggctcctctagaaaagcatagtggggtatctaaaggaggcgg ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~~~ +@HELIUM_000100422_612GNAAXX:7:59:15367:8376#0/2_CONS ali_length=62; direction=left; seq_ab_match=62; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=46; score=247.996374666; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.99994152687; seq_b_insertion=0; +ccgaagtagttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctactacttcgg ++ +ddddddddddddddddddddddddddddddddddddddddddddad~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddedddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:29:1328:13185#0/2_CONS ali_length=63; direction=left; seq_ab_match=59; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=4; seq_a_deletion=0; seq_b_single=45; score=223.94591652; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.55469708763; seq_b_insertion=0; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +ddddddddddddccdddddddddeddddddddddddddddedadd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~W[~~~~~~~~~~~~~~~~~~~~~~~b~~~b~dddcdddddddcddddddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:30:16081:4486#0/2_CONS ali_length=63; direction=left; seq_ab_match=62; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; seq_b_single=45; score=243.950667893; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.87223282369; seq_b_insertion=0; +ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg ++ +ddddddddddddddddddddddddddddddddddddddcdddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~c~~~dddeddddddddddadddddddddddddddddddddddddddddd +@HELIUM_000100422_612GNAAXX:7:104:10274:7999#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; seq_b_single=45; score=251.962499638; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; score_norm=3.99940475616; seq_b_insertion=0; +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg ++ +dddcdddddddddddedddddddddddccdddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddd^ddddddddddddddddddddddddcddddddddddddd +@HELIUM_000100422_612GNAAXX:7:14:7706:12338#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; seq_b_single=46; score=223.864093999; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; score_norm=3.61071119354; seq_b_insertion=0; +ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg ++ +dddcddcddcdbdbddddddddddddddddddcdddddccaddddd~~~~~~b~~~~~~~~~~~x~~~~wyZq~mv~}}~~~~~~~~~P~~~~~~~~~~~~~~~~~~~d^cddddddddcdddadddddddddddddddddddddddddddddd diff -r 000000000000 -r aca6a8c9cf83 test-data/output_obisort.fasta --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/output_obisort.fasta Wed Apr 12 17:38:00 2017 -0400 @@ -0,0 +1,172 @@ +>HELIUM_000100422_612GNAAXX:7:119:14871:19157#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'29a_F260619': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=1; obiclean_samplecount=1; obiclean_head=True; +ttagccctaaacacaagtaattattataacaaaatcattcgccagagtactaccggcaat +agctcaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:108:5640:3823#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'29a_F260619': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=2; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacacaagtaattaatataacaaaattgttcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:97:14311:19299#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'29a_F260619': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=3; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaag +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:24:16230:12703#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'15a_F730814': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=9; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttatcccctt +>HELIUM_000100422_612GNAAXX:7:4:4214:9434#0/2_CONS_SUB_SUB count=1; merged_sample={'13a_F730603': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=10; obiclean_samplecount=1; obiclean_head=False; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactacaggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:13:6954:13039#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=99; obiclean_headcount=0; seq_rank=11; obiclean_samplecount=1; obiclean_head=True; +ttagccctaaacataaacattcaataaacaagaatgttcgccagaggactactagcaata +gcttaaaactcaaaggacttggcggtgctttatatccct +>HELIUM_000100422_612GNAAXX:7:38:6201:12003#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=99; obiclean_headcount=0; seq_rank=12; obiclean_samplecount=1; obiclean_head=True; +ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaata +gcttaaaactcaaagaactgggcggtgctttatatcccg +>HELIUM_000100422_612GNAAXX:7:78:7151:19928#0/2_CONS_SUB_SUB count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=99; obiclean_headcount=0; seq_rank=14; obiclean_samplecount=1; obiclean_head=True; +ttagccctaaacataaacattcaataaacgagaatgttcgccagtgtacttctagcaaca +ggctgaaactcaaaggacttggcggtggtttacatccct +>HELIUM_000100422_612GNAAXX:7:16:16357:19948#0/2_CONS_SUB_SUB count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=99; obiclean_headcount=0; seq_rank=17; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca +gcctgaaactcacaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:107:3570:18147#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'29a_F260619': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=18; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat +agcataaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:114:4511:17596#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'29a_F260619': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=19; obiclean_samplecount=1; obiclean_head=True; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtgctaccggcaat +agcttaaaactcaaaggacttggcggtgctttatacgctt +>HELIUM_000100422_612GNAAXX:7:29:18416:16620#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'29a_F260619': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=21; obiclean_samplecount=1; obiclean_head=True; +ttagccctaaacacaagtaattattataacaaaattattcgccagagttctaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctg +>HELIUM_000100422_612GNAAXX:7:120:1900:13295#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'15a_F730814': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=22; obiclean_samplecount=1; obiclean_head=True; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactacgcgcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:76:10822:18722#0/2_CONS_SUB_SUB count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=99; obiclean_headcount=0; seq_rank=23; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacataaacattcaataaacacgaatgttcgccagagtactactagcaaca +gcctgaaactcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:76:8491:8900#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'15a_F730814': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=24; obiclean_samplecount=1; obiclean_head=True; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtgctacctgcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:9:10358:4089#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'13a_F730603': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=25; obiclean_samplecount=1; obiclean_head=True; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactacaggcaat +agctcaaaactcaaagaacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:59:11971:12063#0/2_CONS_SUB_SUB count=1; merged_sample={'29a_F260619': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=26; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacacaagtaattaatataacaaaattattcgtcagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:111:19168:18517#0/2_CONS_SUB_SUB count=1; merged_sample={'15a_F730814': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=27; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacacaagtaattaatataacaaaattatccgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:41:9316:2256#0/2_CONS_SUB_SUB count=1; merged_sample={'29a_F260619': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=28; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacacaagaaattaatataacaaaattattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:33:9900:9541#0/2_CONS_SUB_SUB count=1; merged_sample={'13a_F730603': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=29; obiclean_samplecount=1; obiclean_head=False; +ctagccctaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:95:1688:9598#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=99; obiclean_headcount=0; seq_rank=30; obiclean_samplecount=1; obiclean_head=True; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtgatactagcaaca +gcctgaaactcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:55:16528:11418#0/2_CONS_SUB_SUB count=1; merged_sample={'13a_F730603': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=31; obiclean_samplecount=1; obiclean_head=True; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcact +agcttacaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:19:6313:16567#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=99; obiclean_headcount=0; seq_rank=32; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca +gcctgaaactcaaagggcttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:111:8478:1127#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=99; obiclean_headcount=0; seq_rank=33; obiclean_samplecount=1; obiclean_head=True; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca +gcctggaacgcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:81:15726:2345#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=99; obiclean_headcount=0; seq_rank=34; obiclean_samplecount=1; obiclean_head=True; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca +gcctgaaactcaaagcactcggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:7:11110:17924#0/2_CONS_SUB_SUB count=1; merged_sample={'15a_F730814': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=35; obiclean_samplecount=1; obiclean_head=True; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtcataccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:89:9710:20652#0/2_CONS_SUB_SUB count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=99; obiclean_headcount=0; seq_rank=36; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca +gcctgaaactcaaaggacttggcggtgctttatatccct +>HELIUM_000100422_612GNAAXX:7:71:17473:7401#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'15a_F730814': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=37; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggtaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:115:3625:10051#0/2_CONS_SUB_SUB count=1; merged_sample={'13a_F730603': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=5; obiclean_headcount=0; seq_rank=38; obiclean_samplecount=1; obiclean_head=True; +caata +>HELIUM_000100422_612GNAAXX:7:99:8183:13912#0/2_CONS_SUB_SUB count=1; merged_sample={'13a_F730603': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=39; obiclean_samplecount=1; obiclean_head=False; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctc +>HELIUM_000100422_612GNAAXX:7:22:1798:3790#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'13a_F730603': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=40; obiclean_samplecount=1; obiclean_head=False; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtgctaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:115:14354:10118#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=99; obiclean_headcount=0; seq_rank=41; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactcgcaaca +gcctgaaactcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:103:3594:15996#0/2_CONS_SUB_SUB count=1; merged_sample={'13a_F730603': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=42; obiclean_samplecount=1; obiclean_head=False; +ctagccttaaacacaaatagttatgcaaacacaactattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:105:14135:6905#0/2_CONS_SUB_SUB count=1; merged_sample={'29a_F260619': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=43; obiclean_samplecount=1; obiclean_head=True; +ttagccctaaacacaagtaattaatataacaaaattattcaccagagtactagcggcaac +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:76:18268:14980#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'29a_F260619': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=44; obiclean_samplecount=1; obiclean_head=False; +ttacccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:25:7789:3090#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'29a_F260619': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=45; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactacaggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:42:19757:3800#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'15a_F730814': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=99; obiclean_headcount=0; seq_rank=46; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacacaagtaattaatatacaaaattattcgccagagtactaccggcaata +gcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:116:14244:17926#0/2_CONS_SUB_SUB count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=99; obiclean_headcount=0; seq_rank=47; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacataaacattcaataaacaagaatgttcgcaagagtactactagcaaca +gcctgaaactcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:72:12959:5736#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=99; obiclean_headcount=0; seq_rank=48; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca +gcctgaaactcaaaggacttggcggtgctttacacccct +>HELIUM_000100422_612GNAAXX:7:7:15538:8325#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=99; obiclean_headcount=0; seq_rank=49; obiclean_samplecount=1; obiclean_head=True; +ttagccctaaacataaacattcaataaacaagaatgtcggccagagtactactagcaaca +gcatgaaactcaaagaactgggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:120:16005:7955#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=98; obiclean_headcount=0; seq_rank=50; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca +gccgaaactcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:10:9237:10532#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'29a_F260619': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=51; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacacaagtaattaatataacaaaataattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:7:11998:4462#0/2_CONS_SUB_SUB count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=99; obiclean_headcount=0; seq_rank=52; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtagtactagcaaca +gcctgaaactcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:111:18277:17779#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=99; obiclean_headcount=0; seq_rank=53; obiclean_samplecount=1; obiclean_head=True; +ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaata +gcctgaaactcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:3:17077:6562#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'15a_F730814': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=54; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactgccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:19:4311:13343#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=99; obiclean_headcount=0; seq_rank=55; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactacgagcaaca +gcctgaaactcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:30:1800:7833#0/2_CONS_SUB_SUB count=1; merged_sample={'15a_F730814': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=56; obiclean_samplecount=1; obiclean_head=True; +ttagccctaaacacaagacattaatataacgagattaatcgacagagtactaccggctat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:108:9222:18258#0/2_CONS_SUB_SUB count=1; merged_sample={'13a_F730603': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=57; obiclean_samplecount=1; obiclean_head=False; +ctagccttaaacacaaatagttatgcagacaaaactattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:38:3005:20881#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=99; obiclean_headcount=0; seq_rank=58; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacatgaacattcaataaacaagaatgttcgccagagtactactagcaaca +gcctgaaactcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:29:15520:18035#0/2_CONS_SUB_SUB count=2; merged_sample={'29a_F260619': 2}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=7; obiclean_samplecount=1; obiclean_head=True; +ttagccctaaacacaagtaattattataacaaaattattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:115:17402:4174#0/2_CONS_SUB_SUB_CMP count=2; merged_sample={'29a_F260619': 1, '15a_F730814': 1}; obiclean_singletoncount=0; obiclean_internalcount=2; seq_length=100; obiclean_headcount=0; seq_rank=13; obiclean_samplecount=2; obiclean_head=False; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactacctgcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:38:10813:16867#0/2_CONS_SUB_SUB count=2; merged_sample={'26a_F040644': 2}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=99; obiclean_headcount=0; seq_rank=15; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacataaacattcaataaacaagaatgtttgccagagtactactagcaaca +gcctgaaactcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:70:11798:2668#0/2_CONS_SUB_SUB count=2; merged_sample={'15a_F730814': 2}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=20; obiclean_samplecount=1; obiclean_head=True; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccttt +>HELIUM_000100422_612GNAAXX:7:89:10281:5930#0/2_CONS_SUB_SUB_CMP count=3; merged_sample={'29a_F260619': 3}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=6; obiclean_samplecount=1; obiclean_head=True; +ttagccctaaacacaaataattacacaaacaaaattgttcaccagagtactagcggcaac +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:13:5742:9859#0/2_CONS_SUB_SUB count=7; merged_sample={'29a_F260619': 7}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=4; obiclean_headcount=0; seq_rank=16; obiclean_samplecount=1; obiclean_head=True; +tttt +>HELIUM_000100422_612GNAAXX:7:6:9274:14951#0/2_CONS_SUB_SUB count=46; merged_sample={'13a_F730603': 46}; obiclean_singletoncount=0; obiclean_internalcount=0; seq_length=100; obiclean_headcount=1; seq_rank=8; obiclean_samplecount=1; obiclean_head=True; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:22:8540:14708#0/2_CONS_SUB_SUB count=61; merged_sample={'29a_F260619': 30, '15a_F730814': 31}; obiclean_singletoncount=0; obiclean_internalcount=0; seq_length=100; obiclean_headcount=2; seq_rank=4; obiclean_samplecount=2; obiclean_head=True; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:57:18459:16145#0/2_CONS_SUB_SUB_CMP count=64; merged_sample={'26a_F040644': 64}; obiclean_singletoncount=0; obiclean_internalcount=0; seq_length=99; obiclean_headcount=1; seq_rank=5; obiclean_samplecount=1; obiclean_head=True; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca +gcctgaaactcaaaggacttggcggtgctttacatccct diff -r 000000000000 -r aca6a8c9cf83 test-data/output_obisort.fastq --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/output_obisort.fastq Wed Apr 12 17:38:00 2017 -0400 @@ -0,0 +1,172 @@ +>HELIUM_000100422_612GNAAXX:7:57:18459:16145#0/2_CONS_SUB_SUB_CMP count=64; merged_sample={'26a_F040644': 64}; obiclean_singletoncount=0; obiclean_internalcount=0; seq_length=99; obiclean_headcount=1; seq_rank=5; obiclean_samplecount=1; obiclean_head=True; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca +gcctgaaactcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:22:8540:14708#0/2_CONS_SUB_SUB count=61; merged_sample={'29a_F260619': 30, '15a_F730814': 31}; obiclean_singletoncount=0; obiclean_internalcount=0; seq_length=100; obiclean_headcount=2; seq_rank=4; obiclean_samplecount=2; obiclean_head=True; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:6:9274:14951#0/2_CONS_SUB_SUB count=46; merged_sample={'13a_F730603': 46}; obiclean_singletoncount=0; obiclean_internalcount=0; seq_length=100; obiclean_headcount=1; seq_rank=8; obiclean_samplecount=1; obiclean_head=True; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:13:5742:9859#0/2_CONS_SUB_SUB count=7; merged_sample={'29a_F260619': 7}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=4; obiclean_headcount=0; seq_rank=16; obiclean_samplecount=1; obiclean_head=True; +tttt +>HELIUM_000100422_612GNAAXX:7:89:10281:5930#0/2_CONS_SUB_SUB_CMP count=3; merged_sample={'29a_F260619': 3}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=6; obiclean_samplecount=1; obiclean_head=True; +ttagccctaaacacaaataattacacaaacaaaattgttcaccagagtactagcggcaac +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:29:15520:18035#0/2_CONS_SUB_SUB count=2; merged_sample={'29a_F260619': 2}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=7; obiclean_samplecount=1; obiclean_head=True; +ttagccctaaacacaagtaattattataacaaaattattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:115:17402:4174#0/2_CONS_SUB_SUB_CMP count=2; merged_sample={'29a_F260619': 1, '15a_F730814': 1}; obiclean_singletoncount=0; obiclean_internalcount=2; seq_length=100; obiclean_headcount=0; seq_rank=13; obiclean_samplecount=2; obiclean_head=False; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactacctgcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:38:10813:16867#0/2_CONS_SUB_SUB count=2; merged_sample={'26a_F040644': 2}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=99; obiclean_headcount=0; seq_rank=15; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacataaacattcaataaacaagaatgtttgccagagtactactagcaaca +gcctgaaactcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:70:11798:2668#0/2_CONS_SUB_SUB count=2; merged_sample={'15a_F730814': 2}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=20; obiclean_samplecount=1; obiclean_head=True; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccttt +>HELIUM_000100422_612GNAAXX:7:119:14871:19157#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'29a_F260619': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=1; obiclean_samplecount=1; obiclean_head=True; +ttagccctaaacacaagtaattattataacaaaatcattcgccagagtactaccggcaat +agctcaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:108:5640:3823#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'29a_F260619': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=2; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacacaagtaattaatataacaaaattgttcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:97:14311:19299#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'29a_F260619': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=3; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaag +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:24:16230:12703#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'15a_F730814': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=9; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttatcccctt +>HELIUM_000100422_612GNAAXX:7:4:4214:9434#0/2_CONS_SUB_SUB count=1; merged_sample={'13a_F730603': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=10; obiclean_samplecount=1; obiclean_head=False; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactacaggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:13:6954:13039#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=99; obiclean_headcount=0; seq_rank=11; obiclean_samplecount=1; obiclean_head=True; +ttagccctaaacataaacattcaataaacaagaatgttcgccagaggactactagcaata +gcttaaaactcaaaggacttggcggtgctttatatccct +>HELIUM_000100422_612GNAAXX:7:38:6201:12003#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=99; obiclean_headcount=0; seq_rank=12; obiclean_samplecount=1; obiclean_head=True; +ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaata +gcttaaaactcaaagaactgggcggtgctttatatcccg +>HELIUM_000100422_612GNAAXX:7:78:7151:19928#0/2_CONS_SUB_SUB count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=99; obiclean_headcount=0; seq_rank=14; obiclean_samplecount=1; obiclean_head=True; +ttagccctaaacataaacattcaataaacgagaatgttcgccagtgtacttctagcaaca +ggctgaaactcaaaggacttggcggtggtttacatccct +>HELIUM_000100422_612GNAAXX:7:16:16357:19948#0/2_CONS_SUB_SUB count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=99; obiclean_headcount=0; seq_rank=17; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca +gcctgaaactcacaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:107:3570:18147#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'29a_F260619': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=18; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat +agcataaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:114:4511:17596#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'29a_F260619': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=19; obiclean_samplecount=1; obiclean_head=True; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtgctaccggcaat +agcttaaaactcaaaggacttggcggtgctttatacgctt +>HELIUM_000100422_612GNAAXX:7:29:18416:16620#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'29a_F260619': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=21; obiclean_samplecount=1; obiclean_head=True; +ttagccctaaacacaagtaattattataacaaaattattcgccagagttctaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctg +>HELIUM_000100422_612GNAAXX:7:120:1900:13295#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'15a_F730814': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=22; obiclean_samplecount=1; obiclean_head=True; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactacgcgcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:76:10822:18722#0/2_CONS_SUB_SUB count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=99; obiclean_headcount=0; seq_rank=23; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacataaacattcaataaacacgaatgttcgccagagtactactagcaaca +gcctgaaactcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:76:8491:8900#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'15a_F730814': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=24; obiclean_samplecount=1; obiclean_head=True; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtgctacctgcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:9:10358:4089#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'13a_F730603': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=25; obiclean_samplecount=1; obiclean_head=True; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactacaggcaat +agctcaaaactcaaagaacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:59:11971:12063#0/2_CONS_SUB_SUB count=1; merged_sample={'29a_F260619': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=26; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacacaagtaattaatataacaaaattattcgtcagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:111:19168:18517#0/2_CONS_SUB_SUB count=1; merged_sample={'15a_F730814': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=27; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacacaagtaattaatataacaaaattatccgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:41:9316:2256#0/2_CONS_SUB_SUB count=1; merged_sample={'29a_F260619': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=28; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacacaagaaattaatataacaaaattattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:33:9900:9541#0/2_CONS_SUB_SUB count=1; merged_sample={'13a_F730603': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=29; obiclean_samplecount=1; obiclean_head=False; +ctagccctaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:95:1688:9598#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=99; obiclean_headcount=0; seq_rank=30; obiclean_samplecount=1; obiclean_head=True; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtgatactagcaaca +gcctgaaactcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:55:16528:11418#0/2_CONS_SUB_SUB count=1; merged_sample={'13a_F730603': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=31; obiclean_samplecount=1; obiclean_head=True; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcact +agcttacaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:19:6313:16567#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=99; obiclean_headcount=0; seq_rank=32; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca +gcctgaaactcaaagggcttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:111:8478:1127#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=99; obiclean_headcount=0; seq_rank=33; obiclean_samplecount=1; obiclean_head=True; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca +gcctggaacgcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:81:15726:2345#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=99; obiclean_headcount=0; seq_rank=34; obiclean_samplecount=1; obiclean_head=True; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca +gcctgaaactcaaagcactcggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:7:11110:17924#0/2_CONS_SUB_SUB count=1; merged_sample={'15a_F730814': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=35; obiclean_samplecount=1; obiclean_head=True; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtcataccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:89:9710:20652#0/2_CONS_SUB_SUB count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=99; obiclean_headcount=0; seq_rank=36; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca +gcctgaaactcaaaggacttggcggtgctttatatccct +>HELIUM_000100422_612GNAAXX:7:71:17473:7401#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'15a_F730814': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=37; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggtaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:115:3625:10051#0/2_CONS_SUB_SUB count=1; merged_sample={'13a_F730603': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=5; obiclean_headcount=0; seq_rank=38; obiclean_samplecount=1; obiclean_head=True; +caata +>HELIUM_000100422_612GNAAXX:7:99:8183:13912#0/2_CONS_SUB_SUB count=1; merged_sample={'13a_F730603': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=39; obiclean_samplecount=1; obiclean_head=False; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctc +>HELIUM_000100422_612GNAAXX:7:22:1798:3790#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'13a_F730603': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=40; obiclean_samplecount=1; obiclean_head=False; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtgctaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:115:14354:10118#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=99; obiclean_headcount=0; seq_rank=41; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactcgcaaca +gcctgaaactcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:103:3594:15996#0/2_CONS_SUB_SUB count=1; merged_sample={'13a_F730603': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=42; obiclean_samplecount=1; obiclean_head=False; +ctagccttaaacacaaatagttatgcaaacacaactattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:105:14135:6905#0/2_CONS_SUB_SUB count=1; merged_sample={'29a_F260619': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=43; obiclean_samplecount=1; obiclean_head=True; +ttagccctaaacacaagtaattaatataacaaaattattcaccagagtactagcggcaac +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:76:18268:14980#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'29a_F260619': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=44; obiclean_samplecount=1; obiclean_head=False; +ttacccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:25:7789:3090#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'29a_F260619': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=45; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactacaggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:42:19757:3800#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'15a_F730814': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=99; obiclean_headcount=0; seq_rank=46; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacacaagtaattaatatacaaaattattcgccagagtactaccggcaata +gcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:116:14244:17926#0/2_CONS_SUB_SUB count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=99; obiclean_headcount=0; seq_rank=47; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacataaacattcaataaacaagaatgttcgcaagagtactactagcaaca +gcctgaaactcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:72:12959:5736#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=99; obiclean_headcount=0; seq_rank=48; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca +gcctgaaactcaaaggacttggcggtgctttacacccct +>HELIUM_000100422_612GNAAXX:7:7:15538:8325#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=99; obiclean_headcount=0; seq_rank=49; obiclean_samplecount=1; obiclean_head=True; +ttagccctaaacataaacattcaataaacaagaatgtcggccagagtactactagcaaca +gcatgaaactcaaagaactgggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:120:16005:7955#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=98; obiclean_headcount=0; seq_rank=50; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca +gccgaaactcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:10:9237:10532#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'29a_F260619': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=51; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacacaagtaattaatataacaaaataattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:7:11998:4462#0/2_CONS_SUB_SUB count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=99; obiclean_headcount=0; seq_rank=52; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtagtactagcaaca +gcctgaaactcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:111:18277:17779#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=99; obiclean_headcount=0; seq_rank=53; obiclean_samplecount=1; obiclean_head=True; +ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaata +gcctgaaactcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:3:17077:6562#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'15a_F730814': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=54; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactgccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:19:4311:13343#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=99; obiclean_headcount=0; seq_rank=55; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactacgagcaaca +gcctgaaactcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:30:1800:7833#0/2_CONS_SUB_SUB count=1; merged_sample={'15a_F730814': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=56; obiclean_samplecount=1; obiclean_head=True; +ttagccctaaacacaagacattaatataacgagattaatcgacagagtactaccggctat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:108:9222:18258#0/2_CONS_SUB_SUB count=1; merged_sample={'13a_F730603': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=57; obiclean_samplecount=1; obiclean_head=False; +ctagccttaaacacaaatagttatgcagacaaaactattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:38:3005:20881#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=99; obiclean_headcount=0; seq_rank=58; obiclean_samplecount=1; obiclean_head=False; +ttagccctaaacatgaacattcaataaacaagaatgttcgccagagtactactagcaaca +gcctgaaactcaaaggacttggcggtgctttacatccct diff -r 000000000000 -r aca6a8c9cf83 test-data/output_obistat.txt --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/output_obistat.txt Wed Apr 12 17:38:00 2017 -0400 @@ -0,0 +1,8 @@ +count merged min_seq_length count total +2 None 99 4 8 +61 None 100 1 61 +46 None 100 1 46 +7 None 4 1 7 +64 None 99 1 64 +1 None 5 49 49 +3 None 100 1 3 diff -r 000000000000 -r aca6a8c9cf83 test-data/output_obitab.txt --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/output_obitab.txt Wed Apr 12 17:38:00 2017 -0400 @@ -0,0 +1,59 @@ +id count sample:13a_F730603 sample:15a_F730814 sample:26a_F040644 sample:29a_F260619 obiclean_head obiclean_headcount obiclean_internalcount obiclean_samplecount obiclean_singletoncount seq_length seq_rank +HELIUM_000100422_612GNAAXX:7:57:18459:16145#0/2_CONS_SUB_SUB_CMP 64 0 0 64 0 True 1 0 1 0 99 5 +HELIUM_000100422_612GNAAXX:7:22:8540:14708#0/2_CONS_SUB_SUB 61 0 31 0 30 True 2 0 2 0 100 4 +HELIUM_000100422_612GNAAXX:7:6:9274:14951#0/2_CONS_SUB_SUB 46 46 0 0 0 True 1 0 1 0 100 8 +HELIUM_000100422_612GNAAXX:7:13:5742:9859#0/2_CONS_SUB_SUB 7 0 0 0 7 True 0 0 1 1 4 16 +HELIUM_000100422_612GNAAXX:7:89:10281:5930#0/2_CONS_SUB_SUB_CMP 3 0 0 0 3 True 0 0 1 1 100 6 +HELIUM_000100422_612GNAAXX:7:29:15520:18035#0/2_CONS_SUB_SUB 2 0 0 0 2 True 0 0 1 1 100 7 +HELIUM_000100422_612GNAAXX:7:115:17402:4174#0/2_CONS_SUB_SUB_CMP 2 0 1 0 1 False 0 2 2 0 100 13 +HELIUM_000100422_612GNAAXX:7:38:10813:16867#0/2_CONS_SUB_SUB 2 0 0 2 0 False 0 1 1 0 99 15 +HELIUM_000100422_612GNAAXX:7:70:11798:2668#0/2_CONS_SUB_SUB 2 0 2 0 0 True 0 0 1 1 100 20 +HELIUM_000100422_612GNAAXX:7:119:14871:19157#0/2_CONS_SUB_SUB_CMP 1 0 0 0 1 True 0 0 1 1 100 1 +HELIUM_000100422_612GNAAXX:7:108:5640:3823#0/2_CONS_SUB_SUB_CMP 1 0 0 0 1 False 0 1 1 0 100 2 +HELIUM_000100422_612GNAAXX:7:97:14311:19299#0/2_CONS_SUB_SUB_CMP 1 0 0 0 1 False 0 1 1 0 100 3 +HELIUM_000100422_612GNAAXX:7:24:16230:12703#0/2_CONS_SUB_SUB_CMP 1 0 1 0 0 False 0 1 1 0 100 9 +HELIUM_000100422_612GNAAXX:7:4:4214:9434#0/2_CONS_SUB_SUB 1 1 0 0 0 False 0 1 1 0 100 10 +HELIUM_000100422_612GNAAXX:7:13:6954:13039#0/2_CONS_SUB_SUB_CMP 1 0 0 1 0 True 0 0 1 1 99 11 +HELIUM_000100422_612GNAAXX:7:38:6201:12003#0/2_CONS_SUB_SUB_CMP 1 0 0 1 0 True 0 0 1 1 99 12 +HELIUM_000100422_612GNAAXX:7:78:7151:19928#0/2_CONS_SUB_SUB 1 0 0 1 0 True 0 0 1 1 99 14 +HELIUM_000100422_612GNAAXX:7:16:16357:19948#0/2_CONS_SUB_SUB 1 0 0 1 0 False 0 1 1 0 99 17 +HELIUM_000100422_612GNAAXX:7:107:3570:18147#0/2_CONS_SUB_SUB_CMP 1 0 0 0 1 False 0 1 1 0 100 18 +HELIUM_000100422_612GNAAXX:7:114:4511:17596#0/2_CONS_SUB_SUB_CMP 1 0 0 0 1 True 0 0 1 1 100 19 +HELIUM_000100422_612GNAAXX:7:29:18416:16620#0/2_CONS_SUB_SUB_CMP 1 0 0 0 1 True 0 0 1 1 100 21 +HELIUM_000100422_612GNAAXX:7:120:1900:13295#0/2_CONS_SUB_SUB_CMP 1 0 1 0 0 True 0 0 1 1 100 22 +HELIUM_000100422_612GNAAXX:7:76:10822:18722#0/2_CONS_SUB_SUB 1 0 0 1 0 False 0 1 1 0 99 23 +HELIUM_000100422_612GNAAXX:7:76:8491:8900#0/2_CONS_SUB_SUB_CMP 1 0 1 0 0 True 0 0 1 1 100 24 +HELIUM_000100422_612GNAAXX:7:9:10358:4089#0/2_CONS_SUB_SUB_CMP 1 1 0 0 0 True 0 0 1 1 100 25 +HELIUM_000100422_612GNAAXX:7:59:11971:12063#0/2_CONS_SUB_SUB 1 0 0 0 1 False 0 1 1 0 100 26 +HELIUM_000100422_612GNAAXX:7:111:19168:18517#0/2_CONS_SUB_SUB 1 0 1 0 0 False 0 1 1 0 100 27 +HELIUM_000100422_612GNAAXX:7:41:9316:2256#0/2_CONS_SUB_SUB 1 0 0 0 1 False 0 1 1 0 100 28 +HELIUM_000100422_612GNAAXX:7:33:9900:9541#0/2_CONS_SUB_SUB 1 1 0 0 0 False 0 1 1 0 100 29 +HELIUM_000100422_612GNAAXX:7:95:1688:9598#0/2_CONS_SUB_SUB_CMP 1 0 0 1 0 True 0 0 1 1 99 30 +HELIUM_000100422_612GNAAXX:7:55:16528:11418#0/2_CONS_SUB_SUB 1 1 0 0 0 True 0 0 1 1 100 31 +HELIUM_000100422_612GNAAXX:7:19:6313:16567#0/2_CONS_SUB_SUB_CMP 1 0 0 1 0 False 0 1 1 0 99 32 +HELIUM_000100422_612GNAAXX:7:111:8478:1127#0/2_CONS_SUB_SUB_CMP 1 0 0 1 0 True 0 0 1 1 99 33 +HELIUM_000100422_612GNAAXX:7:81:15726:2345#0/2_CONS_SUB_SUB_CMP 1 0 0 1 0 True 0 0 1 1 99 34 +HELIUM_000100422_612GNAAXX:7:7:11110:17924#0/2_CONS_SUB_SUB 1 0 1 0 0 True 0 0 1 1 100 35 +HELIUM_000100422_612GNAAXX:7:89:9710:20652#0/2_CONS_SUB_SUB 1 0 0 1 0 False 0 1 1 0 99 36 +HELIUM_000100422_612GNAAXX:7:71:17473:7401#0/2_CONS_SUB_SUB_CMP 1 0 1 0 0 False 0 1 1 0 100 37 +HELIUM_000100422_612GNAAXX:7:115:3625:10051#0/2_CONS_SUB_SUB 1 1 0 0 0 True 0 0 1 1 5 38 +HELIUM_000100422_612GNAAXX:7:99:8183:13912#0/2_CONS_SUB_SUB 1 1 0 0 0 False 0 1 1 0 100 39 +HELIUM_000100422_612GNAAXX:7:22:1798:3790#0/2_CONS_SUB_SUB_CMP 1 1 0 0 0 False 0 1 1 0 100 40 +HELIUM_000100422_612GNAAXX:7:115:14354:10118#0/2_CONS_SUB_SUB_CMP 1 0 0 1 0 False 0 1 1 0 99 41 +HELIUM_000100422_612GNAAXX:7:103:3594:15996#0/2_CONS_SUB_SUB 1 1 0 0 0 False 0 1 1 0 100 42 +HELIUM_000100422_612GNAAXX:7:105:14135:6905#0/2_CONS_SUB_SUB 1 0 0 0 1 True 0 0 1 1 100 43 +HELIUM_000100422_612GNAAXX:7:76:18268:14980#0/2_CONS_SUB_SUB_CMP 1 0 0 0 1 False 0 1 1 0 100 44 +HELIUM_000100422_612GNAAXX:7:25:7789:3090#0/2_CONS_SUB_SUB_CMP 1 0 0 0 1 False 0 1 1 0 100 45 +HELIUM_000100422_612GNAAXX:7:42:19757:3800#0/2_CONS_SUB_SUB_CMP 1 0 1 0 0 False 0 1 1 0 99 46 +HELIUM_000100422_612GNAAXX:7:116:14244:17926#0/2_CONS_SUB_SUB 1 0 0 1 0 False 0 1 1 0 99 47 +HELIUM_000100422_612GNAAXX:7:72:12959:5736#0/2_CONS_SUB_SUB_CMP 1 0 0 1 0 False 0 1 1 0 99 48 +HELIUM_000100422_612GNAAXX:7:7:15538:8325#0/2_CONS_SUB_SUB_CMP 1 0 0 1 0 True 0 0 1 1 99 49 +HELIUM_000100422_612GNAAXX:7:120:16005:7955#0/2_CONS_SUB_SUB_CMP 1 0 0 1 0 False 0 1 1 0 98 50 +HELIUM_000100422_612GNAAXX:7:10:9237:10532#0/2_CONS_SUB_SUB_CMP 1 0 0 0 1 False 0 1 1 0 100 51 +HELIUM_000100422_612GNAAXX:7:7:11998:4462#0/2_CONS_SUB_SUB 1 0 0 1 0 False 0 1 1 0 99 52 +HELIUM_000100422_612GNAAXX:7:111:18277:17779#0/2_CONS_SUB_SUB_CMP 1 0 0 1 0 True 0 0 1 1 99 53 +HELIUM_000100422_612GNAAXX:7:3:17077:6562#0/2_CONS_SUB_SUB_CMP 1 0 1 0 0 False 0 1 1 0 100 54 +HELIUM_000100422_612GNAAXX:7:19:4311:13343#0/2_CONS_SUB_SUB_CMP 1 0 0 1 0 False 0 1 1 0 99 55 +HELIUM_000100422_612GNAAXX:7:30:1800:7833#0/2_CONS_SUB_SUB 1 0 1 0 0 True 0 0 1 1 100 56 +HELIUM_000100422_612GNAAXX:7:108:9222:18258#0/2_CONS_SUB_SUB 1 1 0 0 0 False 0 1 1 0 100 57 +HELIUM_000100422_612GNAAXX:7:38:3005:20881#0/2_CONS_SUB_SUB_CMP 1 0 0 1 0 False 0 1 1 0 99 58 diff -r 000000000000 -r aca6a8c9cf83 test-data/output_obiuniq.fasta --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/output_obiuniq.fasta Wed Apr 12 17:38:00 2017 -0400 @@ -0,0 +1,172 @@ +>HELIUM_000100422_612GNAAXX:7:119:14871:19157#0/2_CONS_SUB_SUB_CMP ali_length=62; seq_ab_match=47; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; merged_sample={'29a_F260619': 1}; forward_score=72.0; score=115.761290673; seq_a_mismatch=1; forward_tag=gcctcct; seq_b_mismatch=13; experiment=wolf_diet; mid_quality=69.1492537313; avg_quality=68.8701298701; seq_a_single=46; score_norm=1.86711759151; status=full; direction=reverse; seq_b_insertion=1; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=154; reverse_tag=gcctcct; count=1; seq_length=100; mode=alignment; head_quality=67.0; seq_b_single=46; +ttagccctaaacacaagtaattattataacaaaatcattcgccagagtactaccggcaat +agctcaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:108:5640:3823#0/2_CONS_SUB_SUB_CMP ali_length=62; seq_ab_match=60; sminR=40.0; tail_quality=66.9; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; merged_sample={'29a_F260619': 1}; forward_score=72.0; score=231.881364714; seq_a_mismatch=0; forward_tag=gcctcct; seq_b_mismatch=2; experiment=wolf_diet; mid_quality=78.447761194; avg_quality=76.9545454545; seq_a_single=46; score_norm=3.74002201152; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=gcctcct; count=1; seq_length=100; mode=alignment; head_quality=67.0; seq_b_single=46; +ttagccctaaacacaagtaattaatataacaaaattgttcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:97:14311:19299#0/2_CONS_SUB_SUB_CMP ali_length=62; seq_ab_match=52; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; merged_sample={'29a_F260619': 1}; forward_score=72.0; score=167.91734192; seq_a_mismatch=5; forward_tag=gcctcct; seq_b_mismatch=5; experiment=wolf_diet; mid_quality=75.0820895522; avg_quality=74.0064935065; seq_a_single=46; score_norm=2.70834422451; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=gcctcct; count=1; seq_length=100; mode=alignment; head_quality=66.6; seq_b_single=46; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaag +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:22:8540:14708#0/2_CONS_SUB_SUB sminR=40.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; merged_sample={'29a_F260619': 30, '15a_F730814': 31}; forward_score=72.0; experiment=wolf_diet; reverse_score=72.0; seq_length_ori=154; count=61; seq_length=100; status=full; mode=alignment; seq_b_single=46; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:57:18459:16145#0/2_CONS_SUB_SUB_CMP ali_length=63; sminR=40.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; merged_sample={'26a_F040644': 64}; forward_score=72.0; forward_tag=gaatatc; experiment=wolf_diet; seq_a_single=45; reverse_score=72.0; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=153; reverse_tag=gaatatc; count=64; seq_length=99; status=full; mode=alignment; seq_b_single=45; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca +gcctgaaactcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:89:10281:5930#0/2_CONS_SUB_SUB_CMP ali_length=62; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; merged_sample={'29a_F260619': 3}; forward_score=72.0; seq_a_mismatch=2; forward_tag=gcctcct; experiment=wolf_diet; seq_a_single=46; reverse_score=72.0; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=gcctcct; count=3; seq_length=100; status=full; mode=alignment; seq_b_single=46; +ttagccctaaacacaaataattacacaaacaaaattgttcaccagagtactagcggcaac +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:29:15520:18035#0/2_CONS_SUB_SUB ali_length=62; sminR=40.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; merged_sample={'29a_F260619': 2}; forward_score=72.0; forward_tag=gcctcct; experiment=wolf_diet; seq_a_single=46; reverse_score=72.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=gcctcct; count=2; seq_length=100; status=full; mode=alignment; seq_b_single=46; +ttagccctaaacacaagtaattattataacaaaattattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:6:9274:14951#0/2_CONS_SUB_SUB sminR=40.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; merged_sample={'13a_F730603': 46}; forward_score=72.0; forward_tag=aattaac; experiment=wolf_diet; reverse_score=72.0; seq_b_deletion=0; seq_a_insertion=0; reverse_tag=aattaac; count=46; seq_length=100; status=full; mode=alignment; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:24:16230:12703#0/2_CONS_SUB_SUB_CMP ali_length=62; seq_ab_match=59; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; merged_sample={'15a_F730814': 1}; forward_score=72.0; score=223.86908915; seq_a_mismatch=2; forward_tag=gaagtag; seq_b_mismatch=1; experiment=wolf_diet; mid_quality=77.4552238806; avg_quality=76.0519480519; seq_a_single=46; score_norm=3.61079176049; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=gaagtag; count=1; seq_length=100; mode=alignment; head_quality=66.3; seq_b_single=46; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttatcccctt +>HELIUM_000100422_612GNAAXX:7:4:4214:9434#0/2_CONS_SUB_SUB ali_length=62; seq_ab_match=58; sminR=40.0; tail_quality=63.3; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; merged_sample={'13a_F730603': 1}; forward_score=72.0; score=215.812703412; seq_a_mismatch=3; forward_tag=aattaac; seq_b_mismatch=1; experiment=wolf_diet; mid_quality=76.2388059701; avg_quality=74.6883116883; seq_a_single=46; score_norm=3.48085005502; status=full; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=aattaac; count=1; seq_length=100; mode=alignment; head_quality=65.3; seq_b_single=46; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactacaggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:13:6954:13039#0/2_CONS_SUB_SUB_CMP ali_length=63; seq_ab_match=62; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; merged_sample={'26a_F040644': 1}; forward_score=72.0; score=243.872474161; seq_a_mismatch=1; forward_tag=gaatatc; seq_b_mismatch=0; experiment=wolf_diet; mid_quality=79.0; avg_quality=77.431372549; seq_a_single=45; score_norm=3.87099165336; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=153; reverse_tag=gaatatc; count=1; seq_length=99; mode=alignment; head_quality=67.0; seq_b_single=45; +ttagccctaaacataaacattcaataaacaagaatgttcgccagaggactactagcaata +gcttaaaactcaaaggacttggcggtgctttatatccct +>HELIUM_000100422_612GNAAXX:7:38:6201:12003#0/2_CONS_SUB_SUB_CMP ali_length=63; seq_ab_match=59; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; merged_sample={'26a_F040644': 1}; forward_score=72.0; score=219.686655506; seq_a_mismatch=0; forward_tag=gaatatc; seq_b_mismatch=4; experiment=wolf_diet; mid_quality=67.7443609023; avg_quality=67.3986928105; seq_a_single=45; score_norm=3.48708976993; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=153; reverse_tag=gaatatc; count=1; seq_length=99; mode=alignment; head_quality=63.2; seq_b_single=45; +ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaata +gcttaaaactcaaagaactgggcggtgctttatatcccg +>HELIUM_000100422_612GNAAXX:7:115:17402:4174#0/2_CONS_SUB_SUB_CMP ali_length=62; sminR=40.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; merged_sample={'29a_F260619': 1, '15a_F730814': 1}; forward_score=72.0; seq_b_mismatch=0; experiment=wolf_diet; seq_a_single=46; reverse_score=72.0; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; count=2; seq_length=100; status=full; mode=alignment; seq_b_single=46; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactacctgcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:78:7151:19928#0/2_CONS_SUB_SUB ali_length=63; seq_ab_match=56; sminR=40.0; tail_quality=47.6; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; merged_sample={'26a_F040644': 1}; forward_score=72.0; score=195.576462373; seq_a_mismatch=0; forward_tag=gaatatc; seq_b_mismatch=7; experiment=wolf_diet; mid_quality=58.2105263158; avg_quality=57.1699346405; seq_a_single=45; score_norm=3.10438829164; status=full; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=153; reverse_tag=gaatatc; count=1; seq_length=99; mode=alignment; head_quality=52.9; seq_b_single=45; +ttagccctaaacataaacattcaataaacgagaatgttcgccagtgtacttctagcaaca +ggctgaaactcaaaggacttggcggtggtttacatccct +>HELIUM_000100422_612GNAAXX:7:38:10813:16867#0/2_CONS_SUB_SUB ali_length=63; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; merged_sample={'26a_F040644': 2}; forward_score=72.0; forward_tag=gaatatc; seq_b_mismatch=0; experiment=wolf_diet; seq_a_single=45; reverse_score=72.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=153; reverse_tag=gaatatc; count=2; seq_length=99; status=full; mode=alignment; head_quality=67.0; seq_b_single=45; +ttagccctaaacataaacattcaataaacaagaatgtttgccagagtactactagcaaca +gcctgaaactcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:13:5742:9859#0/2_CONS_SUB_SUB ali_length=58; sminR=40.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=50; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; merged_sample={'29a_F260619': 7}; forward_score=72.0; forward_tag=gcctcct; experiment=wolf_diet; seq_a_single=0; reverse_score=72.0; seq_b_insertion=0; seq_b_deletion=50; seq_a_insertion=0; seq_length_ori=58; reverse_tag=gcctcct; count=7; seq_length=4; status=full; mode=alignment; seq_b_single=0; +tttt +>HELIUM_000100422_612GNAAXX:7:16:16357:19948#0/2_CONS_SUB_SUB ali_length=63; seq_ab_match=57; sminR=40.0; tail_quality=66.7; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; merged_sample={'26a_F040644': 1}; forward_score=72.0; score=203.860719115; seq_a_mismatch=5; forward_tag=gaatatc; seq_b_mismatch=1; experiment=wolf_diet; mid_quality=75.6541353383; avg_quality=74.1764705882; seq_a_single=45; score_norm=3.23588443039; status=full; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=153; reverse_tag=gaatatc; count=1; seq_length=99; mode=alignment; head_quality=62.0; seq_b_single=45; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca +gcctgaaactcacaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:107:3570:18147#0/2_CONS_SUB_SUB_CMP ali_length=62; seq_ab_match=60; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; merged_sample={'29a_F260619': 1}; forward_score=72.0; score=231.924768153; seq_a_mismatch=2; forward_tag=gcctcct; seq_b_mismatch=0; experiment=wolf_diet; mid_quality=78.0820895522; avg_quality=76.5714285714; seq_a_single=46; score_norm=3.74072206698; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=gcctcct; count=1; seq_length=100; mode=alignment; head_quality=65.9; seq_b_single=46; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat +agcataaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:114:4511:17596#0/2_CONS_SUB_SUB_CMP ali_length=62; seq_ab_match=57; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; merged_sample={'29a_F260619': 1}; forward_score=72.0; score=207.883730153; seq_a_mismatch=4; forward_tag=gcctcct; seq_b_mismatch=1; experiment=wolf_diet; mid_quality=75.3208955224; avg_quality=74.2532467532; seq_a_single=46; score_norm=3.35296338956; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=gcctcct; count=1; seq_length=100; mode=alignment; head_quality=67.2; seq_b_single=46; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtgctaccggcaat +agcttaaaactcaaaggacttggcggtgctttatacgctt +>HELIUM_000100422_612GNAAXX:7:70:11798:2668#0/2_CONS_SUB_SUB ali_length=62; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; merged_sample={'15a_F730814': 2}; forward_score=72.0; seq_a_mismatch=1; forward_tag=gaagtag; experiment=wolf_diet; seq_a_single=46; reverse_score=72.0; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=gaagtag; count=2; seq_length=100; status=full; mode=alignment; seq_b_single=46; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccttt +>HELIUM_000100422_612GNAAXX:7:29:18416:16620#0/2_CONS_SUB_SUB_CMP ali_length=62; seq_ab_match=60; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; merged_sample={'29a_F260619': 1}; forward_score=72.0; score=231.873033258; seq_a_mismatch=2; forward_tag=gcctcct; seq_b_mismatch=0; experiment=wolf_diet; mid_quality=77.4179104478; avg_quality=76.0649350649; seq_a_single=46; score_norm=3.7398876332; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=gcctcct; count=1; seq_length=100; mode=alignment; head_quality=67.0; seq_b_single=46; +ttagccctaaacacaagtaattattataacaaaattattcgccagagttctaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctg +>HELIUM_000100422_612GNAAXX:7:120:1900:13295#0/2_CONS_SUB_SUB_CMP ali_length=62; seq_ab_match=60; sminR=40.0; tail_quality=67.1; reverse_match=tagaacaggctcctctag; seq_a_deletion=1; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; merged_sample={'15a_F730814': 1}; forward_score=72.0; score=219.874676069; seq_a_mismatch=1; forward_tag=gaagtag; seq_b_mismatch=0; experiment=wolf_diet; mid_quality=77.5149253731; avg_quality=76.1558441558; seq_a_single=46; score_norm=3.54636574304; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=1; seq_length_ori=154; reverse_tag=gaagtag; count=1; seq_length=100; mode=alignment; head_quality=67.0; seq_b_single=46; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactacgcgcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:76:10822:18722#0/2_CONS_SUB_SUB ali_length=63; seq_ab_match=58; sminR=40.0; tail_quality=66.8; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; merged_sample={'26a_F040644': 1}; forward_score=72.0; score=211.83390837; seq_a_mismatch=5; forward_tag=gaatatc; seq_b_mismatch=0; experiment=wolf_diet; mid_quality=77.3834586466; avg_quality=75.9803921569; seq_a_single=45; score_norm=3.36244299; status=full; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=153; reverse_tag=gaatatc; count=1; seq_length=99; mode=alignment; head_quality=66.5; seq_b_single=45; +ttagccctaaacataaacattcaataaacacgaatgttcgccagagtactactagcaaca +gcctgaaactcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:76:8491:8900#0/2_CONS_SUB_SUB_CMP ali_length=62; seq_ab_match=57; sminR=40.0; tail_quality=65.3; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; merged_sample={'15a_F730814': 1}; forward_score=72.0; score=207.828655885; seq_a_mismatch=4; forward_tag=gaagtag; seq_b_mismatch=1; experiment=wolf_diet; mid_quality=76.552238806; avg_quality=75.1948051948; seq_a_single=46; score_norm=3.35207509492; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=gaagtag; count=1; seq_length=100; mode=alignment; head_quality=66.9; seq_b_single=46; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtgctacctgcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:9:10358:4089#0/2_CONS_SUB_SUB_CMP ali_length=61; seq_ab_match=54; sminR=40.0; tail_quality=66.9; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; merged_sample={'13a_F730603': 1}; forward_score=72.0; score=181.620394786; seq_a_mismatch=1; forward_tag=aattaac; seq_b_mismatch=6; experiment=wolf_diet; mid_quality=66.3880597015; avg_quality=66.4545454545; seq_a_single=46; score_norm=2.97738352108; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=154; reverse_tag=aattaac; count=1; seq_length=100; mode=alignment; head_quality=66.9; seq_b_single=47; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactacaggcaat +agctcaaaactcaaagaacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:59:11971:12063#0/2_CONS_SUB_SUB ali_length=62; seq_ab_match=62; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; merged_sample={'29a_F260619': 1}; forward_score=72.0; score=247.995051728; seq_a_mismatch=0; forward_tag=gcctcct; seq_b_mismatch=0; experiment=wolf_diet; mid_quality=78.9850746269; avg_quality=77.4285714286; seq_a_single=46; score_norm=3.99992018915; status=full; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=gcctcct; count=1; seq_length=100; mode=alignment; head_quality=67.0; seq_b_single=46; +ttagccctaaacacaagtaattaatataacaaaattattcgtcagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:111:19168:18517#0/2_CONS_SUB_SUB ali_length=63; seq_ab_match=60; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; merged_sample={'15a_F730814': 1}; forward_score=72.0; score=223.825469474; seq_a_mismatch=1; forward_tag=gaagtag; seq_b_mismatch=1; experiment=wolf_diet; mid_quality=77.8955223881; avg_quality=76.4805194805; seq_a_single=45; score_norm=3.55278522975; status=full; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=1; seq_length_ori=154; reverse_tag=gaagtag; count=1; seq_length=100; mode=alignment; head_quality=67.0; seq_b_single=46; +ttagccctaaacacaagtaattaatataacaaaattatccgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:41:9316:2256#0/2_CONS_SUB_SUB ali_length=62; seq_ab_match=61; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; merged_sample={'29a_F260619': 1}; forward_score=72.0; score=239.993848494; seq_a_mismatch=1; forward_tag=gcctcct; seq_b_mismatch=0; experiment=wolf_diet; mid_quality=78.6343283582; avg_quality=77.1233766234; seq_a_single=46; score_norm=3.87086852409; status=full; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=gcctcct; count=1; seq_length=100; mode=alignment; head_quality=67.0; seq_b_single=46; +ttagccctaaacacaagaaattaatataacaaaattattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:33:9900:9541#0/2_CONS_SUB_SUB ali_length=62; seq_ab_match=62; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; merged_sample={'13a_F730603': 1}; forward_score=72.0; score=247.949713348; seq_a_mismatch=0; forward_tag=aattaac; seq_b_mismatch=0; experiment=wolf_diet; mid_quality=78.8955223881; avg_quality=77.3506493506; seq_a_single=46; score_norm=3.99918892497; status=full; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=aattaac; count=1; seq_length=100; mode=alignment; head_quality=67.0; seq_b_single=46; +ctagccctaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:95:1688:9598#0/2_CONS_SUB_SUB_CMP ali_length=63; seq_ab_match=54; sminR=40.0; tail_quality=66.8; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; merged_sample={'26a_F040644': 1}; forward_score=72.0; score=179.847313607; seq_a_mismatch=6; forward_tag=gaatatc; seq_b_mismatch=3; experiment=wolf_diet; mid_quality=74.2932330827; avg_quality=73.2418300654; seq_a_single=45; score_norm=2.8547192636; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=153; reverse_tag=gaatatc; count=1; seq_length=99; mode=alignment; head_quality=65.7; seq_b_single=45; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtgatactagcaaca +gcctgaaactcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:55:16528:11418#0/2_CONS_SUB_SUB ali_length=62; seq_ab_match=58; sminR=40.0; tail_quality=57.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; merged_sample={'13a_F730603': 1}; forward_score=72.0; score=215.988419267; seq_a_mismatch=4; forward_tag=aattaac; seq_b_mismatch=0; experiment=wolf_diet; mid_quality=76.0746268657; avg_quality=74.2142857143; seq_a_single=46; score_norm=3.48368418172; status=full; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=aattaac; count=1; seq_length=100; mode=alignment; head_quality=66.5; seq_b_single=46; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcact +agcttacaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:19:6313:16567#0/2_CONS_SUB_SUB_CMP ali_length=63; seq_ab_match=60; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; merged_sample={'26a_F040644': 1}; forward_score=72.0; score=227.826165596; seq_a_mismatch=3; forward_tag=gaatatc; seq_b_mismatch=0; experiment=wolf_diet; mid_quality=77.4135338346; avg_quality=76.0261437908; seq_a_single=45; score_norm=3.61628834279; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=153; reverse_tag=gaatatc; count=1; seq_length=99; mode=alignment; head_quality=66.6; seq_b_single=45; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca +gcctgaaactcaaagggcttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:111:8478:1127#0/2_CONS_SUB_SUB_CMP ali_length=63; seq_ab_match=57; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; merged_sample={'26a_F040644': 1}; forward_score=72.0; score=203.794981474; seq_a_mismatch=5; forward_tag=gaatatc; seq_b_mismatch=1; experiment=wolf_diet; mid_quality=76.3909774436; avg_quality=75.1241830065; seq_a_single=45; score_norm=3.23484097578; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=153; reverse_tag=gaatatc; count=1; seq_length=99; mode=alignment; head_quality=66.4; seq_b_single=45; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca +gcctggaacgcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:81:15726:2345#0/2_CONS_SUB_SUB_CMP ali_length=63; seq_ab_match=56; sminR=40.0; tail_quality=62.3; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; merged_sample={'26a_F040644': 1}; forward_score=72.0; score=195.641193686; seq_a_mismatch=0; forward_tag=gaatatc; seq_b_mismatch=7; experiment=wolf_diet; mid_quality=63.1954887218; avg_quality=63.091503268; seq_a_single=45; score_norm=3.10541577279; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=153; reverse_tag=gaatatc; count=1; seq_length=99; mode=alignment; head_quality=62.5; seq_b_single=45; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca +gcctgaaactcaaagcactcggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:7:11110:17924#0/2_CONS_SUB_SUB ali_length=62; seq_ab_match=58; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; merged_sample={'15a_F730814': 1}; forward_score=72.0; score=215.812113201; seq_a_mismatch=3; forward_tag=gaagtag; seq_b_mismatch=1; experiment=wolf_diet; mid_quality=75.723880597; avg_quality=74.5519480519; seq_a_single=46; score_norm=3.4808405355; status=full; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=gaagtag; count=1; seq_length=100; mode=alignment; head_quality=66.4; seq_b_single=46; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtcataccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:89:9710:20652#0/2_CONS_SUB_SUB ali_length=63; seq_ab_match=62; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; merged_sample={'26a_F040644': 1}; forward_score=72.0; score=243.910293117; seq_a_mismatch=0; forward_tag=gaatatc; seq_b_mismatch=1; experiment=wolf_diet; mid_quality=78.8421052632; avg_quality=77.2941176471; seq_a_single=45; score_norm=3.87159195424; status=full; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=153; reverse_tag=gaatatc; count=1; seq_length=99; mode=alignment; head_quality=67.0; seq_b_single=45; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca +gcctgaaactcaaaggacttggcggtgctttatatccct +>HELIUM_000100422_612GNAAXX:7:71:17473:7401#0/2_CONS_SUB_SUB_CMP ali_length=62; seq_ab_match=60; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; merged_sample={'15a_F730814': 1}; forward_score=72.0; score=231.883938289; seq_a_mismatch=1; forward_tag=gaagtag; seq_b_mismatch=1; experiment=wolf_diet; mid_quality=77.2089552239; avg_quality=75.7857142857; seq_a_single=46; score_norm=3.74006352079; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=gaagtag; count=1; seq_length=100; mode=alignment; head_quality=65.5; seq_b_single=46; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggtaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:115:3625:10051#0/2_CONS_SUB_SUB ali_length=59; seq_ab_match=59; sminR=40.0; tail_quality=93.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=49; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; merged_sample={'13a_F730603': 1}; forward_score=72.0; score=235.999642017; seq_a_mismatch=0; forward_tag=aattaac; seq_b_mismatch=0; experiment=wolf_diet; mid_quality=93.0; avg_quality=93.0; seq_a_single=0; score_norm=3.99999393249; status=full; direction=forward; seq_b_insertion=0; seq_b_deletion=49; seq_a_insertion=0; seq_length_ori=59; reverse_tag=aattaac; count=1; seq_length=5; mode=alignment; head_quality=93.0; seq_b_single=0; +caata +>HELIUM_000100422_612GNAAXX:7:99:8183:13912#0/2_CONS_SUB_SUB ali_length=62; seq_ab_match=60; sminR=40.0; tail_quality=67.1; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; merged_sample={'13a_F730603': 1}; forward_score=72.0; score=231.903850642; seq_a_mismatch=1; forward_tag=aattaac; seq_b_mismatch=1; experiment=wolf_diet; mid_quality=78.3358208955; avg_quality=76.8701298701; seq_a_single=46; score_norm=3.74038468777; status=full; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=aattaac; count=1; seq_length=100; mode=alignment; head_quality=67.0; seq_b_single=46; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctc +>HELIUM_000100422_612GNAAXX:7:22:1798:3790#0/2_CONS_SUB_SUB_CMP ali_length=62; seq_ab_match=59; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; merged_sample={'13a_F730603': 1}; forward_score=72.0; score=223.82253574; seq_a_mismatch=3; forward_tag=aattaac; seq_b_mismatch=0; experiment=wolf_diet; mid_quality=78.3208955224; avg_quality=76.8506493506; seq_a_single=46; score_norm=3.61004089903; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=aattaac; count=1; seq_length=100; mode=alignment; head_quality=67.0; seq_b_single=46; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtgctaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:115:14354:10118#0/2_CONS_SUB_SUB_CMP ali_length=63; seq_ab_match=59; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; merged_sample={'26a_F040644': 1}; forward_score=72.0; score=219.850378027; seq_a_mismatch=2; forward_tag=gaatatc; seq_b_mismatch=2; experiment=wolf_diet; mid_quality=78.045112782; avg_quality=76.6013071895; seq_a_single=45; score_norm=3.4896885401; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=153; reverse_tag=gaatatc; count=1; seq_length=99; mode=alignment; head_quality=67.0; seq_b_single=45; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactcgcaaca +gcctgaaactcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:103:3594:15996#0/2_CONS_SUB_SUB ali_length=62; seq_ab_match=60; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; merged_sample={'13a_F730603': 1}; forward_score=72.0; score=231.92194325; seq_a_mismatch=2; forward_tag=aattaac; seq_b_mismatch=0; experiment=wolf_diet; mid_quality=78.3805970149; avg_quality=76.9090909091; seq_a_single=46; score_norm=3.74067650404; status=full; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=aattaac; count=1; seq_length=100; mode=alignment; head_quality=67.1; seq_b_single=46; +ctagccttaaacacaaatagttatgcaaacacaactattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:105:14135:6905#0/2_CONS_SUB_SUB ali_length=62; seq_ab_match=61; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; merged_sample={'29a_F260619': 1}; forward_score=72.0; score=239.985379811; seq_a_mismatch=1; forward_tag=gcctcct; seq_b_mismatch=0; experiment=wolf_diet; mid_quality=78.8134328358; avg_quality=77.2792207792; seq_a_single=46; score_norm=3.87073193244; status=full; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=gcctcct; count=1; seq_length=100; mode=alignment; head_quality=67.0; seq_b_single=46; +ttagccctaaacacaagtaattaatataacaaaattattcaccagagtactagcggcaac +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:76:18268:14980#0/2_CONS_SUB_SUB_CMP ali_length=62; seq_ab_match=61; sminR=40.0; tail_quality=66.9; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; merged_sample={'29a_F260619': 1}; forward_score=72.0; score=239.985254914; seq_a_mismatch=1; forward_tag=gcctcct; seq_b_mismatch=0; experiment=wolf_diet; mid_quality=78.3208955224; avg_quality=76.8441558442; seq_a_single=46; score_norm=3.87072991797; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=gcctcct; count=1; seq_length=100; mode=alignment; head_quality=67.0; seq_b_single=46; +ttacccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:25:7789:3090#0/2_CONS_SUB_SUB_CMP ali_length=62; seq_ab_match=59; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; merged_sample={'29a_F260619': 1}; forward_score=72.0; score=223.705772044; seq_a_mismatch=0; forward_tag=gcctcct; seq_b_mismatch=3; experiment=wolf_diet; mid_quality=72.4179104478; avg_quality=71.6818181818; seq_a_single=46; score_norm=3.60815761361; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=gcctcct; count=1; seq_length=100; mode=alignment; head_quality=66.5; seq_b_single=46; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactacaggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:42:19757:3800#0/2_CONS_SUB_SUB_CMP ali_length=62; seq_ab_match=57; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=1; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; merged_sample={'15a_F730814': 1}; forward_score=72.0; score=187.78301321; seq_a_mismatch=1; forward_tag=gaagtag; seq_b_mismatch=3; experiment=wolf_diet; mid_quality=77.7669172932; avg_quality=76.3660130719; seq_a_single=45; score_norm=3.02875827758; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=1; seq_a_insertion=1; seq_length_ori=153; reverse_tag=gaagtag; count=1; seq_length=99; mode=alignment; head_quality=67.1; seq_b_single=46; +ttagccctaaacacaagtaattaatatacaaaattattcgccagagtactaccggcaata +gcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:116:14244:17926#0/2_CONS_SUB_SUB ali_length=63; seq_ab_match=55; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; merged_sample={'26a_F040644': 1}; forward_score=72.0; score=187.882758912; seq_a_mismatch=5; forward_tag=gaatatc; seq_b_mismatch=3; experiment=wolf_diet; mid_quality=76.5338345865; avg_quality=75.2875816993; seq_a_single=45; score_norm=2.98226601448; status=full; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=153; reverse_tag=gaatatc; count=1; seq_length=99; mode=alignment; head_quality=67.0; seq_b_single=45; +ttagccctaaacataaacattcaataaacaagaatgttcgcaagagtactactagcaaca +gcctgaaactcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:72:12959:5736#0/2_CONS_SUB_SUB_CMP ali_length=63; seq_ab_match=56; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; merged_sample={'26a_F040644': 1}; forward_score=72.0; score=195.664863172; seq_a_mismatch=0; forward_tag=gaatatc; seq_b_mismatch=7; experiment=wolf_diet; mid_quality=63.5263157895; avg_quality=63.5098039216; seq_a_single=45; score_norm=3.10579147892; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=153; reverse_tag=gaatatc; count=1; seq_length=99; mode=alignment; head_quality=59.8; seq_b_single=45; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca +gcctgaaactcaaaggacttggcggtgctttacacccct +>HELIUM_000100422_612GNAAXX:7:7:15538:8325#0/2_CONS_SUB_SUB_CMP ali_length=63; seq_ab_match=52; sminR=40.0; tail_quality=63.4; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; merged_sample={'26a_F040644': 1}; forward_score=72.0; score=151.662770749; seq_a_mismatch=0; forward_tag=gaatatc; seq_b_mismatch=10; experiment=wolf_diet; mid_quality=60.1879699248; avg_quality=60.3333333333; seq_a_single=45; score_norm=2.40734556744; status=full; direction=reverse; seq_b_insertion=1; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=153; reverse_tag=gaatatc; count=1; seq_length=99; mode=alignment; head_quality=59.2; seq_b_single=45; +ttagccctaaacataaacattcaataaacaagaatgtcggccagagtactactagcaaca +gcatgaaactcaaagaactgggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:120:16005:7955#0/2_CONS_SUB_SUB_CMP ali_length=64; seq_ab_match=61; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; merged_sample={'26a_F040644': 1}; forward_score=72.0; score=231.880428082; seq_a_mismatch=1; forward_tag=gaatatc; seq_b_mismatch=2; experiment=wolf_diet; mid_quality=78.8939393939; avg_quality=77.3289473684; seq_a_single=44; score_norm=3.62313168878; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=152; reverse_tag=gaatatc; count=1; seq_length=98; mode=alignment; head_quality=67.0; seq_b_single=44; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca +gccgaaactcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:10:9237:10532#0/2_CONS_SUB_SUB_CMP ali_length=62; seq_ab_match=57; sminR=40.0; tail_quality=66.9; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; merged_sample={'29a_F260619': 1}; forward_score=72.0; score=207.90944986; seq_a_mismatch=2; forward_tag=gcctcct; seq_b_mismatch=3; experiment=wolf_diet; mid_quality=75.9402985075; avg_quality=74.7532467532; seq_a_single=46; score_norm=3.35337822355; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=gcctcct; count=1; seq_length=100; mode=alignment; head_quality=66.7; seq_b_single=46; +ttagccctaaacacaagtaattaatataacaaaataattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:7:11998:4462#0/2_CONS_SUB_SUB ali_length=63; seq_ab_match=59; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; merged_sample={'26a_F040644': 1}; forward_score=72.0; score=219.864332815; seq_a_mismatch=4; forward_tag=gaatatc; seq_b_mismatch=0; experiment=wolf_diet; mid_quality=78.2105263158; avg_quality=76.7450980392; seq_a_single=45; score_norm=3.48991004469; status=full; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=153; reverse_tag=gaatatc; count=1; seq_length=99; mode=alignment; head_quality=67.0; seq_b_single=45; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtagtactagcaaca +gcctgaaactcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:111:18277:17779#0/2_CONS_SUB_SUB_CMP ali_length=63; seq_ab_match=61; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; merged_sample={'26a_F040644': 1}; forward_score=72.0; score=235.877819976; seq_a_mismatch=2; forward_tag=gaatatc; seq_b_mismatch=0; experiment=wolf_diet; mid_quality=78.8646616541; avg_quality=77.3137254902; seq_a_single=45; score_norm=3.74409238057; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=153; reverse_tag=gaatatc; count=1; seq_length=99; mode=alignment; head_quality=67.0; seq_b_single=45; +ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaata +gcctgaaactcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:3:17077:6562#0/2_CONS_SUB_SUB_CMP ali_length=62; seq_ab_match=59; sminR=40.0; tail_quality=66.6; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; merged_sample={'15a_F730814': 1}; forward_score=72.0; score=223.876050008; seq_a_mismatch=3; forward_tag=gaagtag; seq_b_mismatch=0; experiment=wolf_diet; mid_quality=77.6567164179; avg_quality=76.1818181818; seq_a_single=46; score_norm=3.61090403239; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=gaagtag; count=1; seq_length=100; mode=alignment; head_quality=66.0; seq_b_single=46; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactgccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:19:4311:13343#0/2_CONS_SUB_SUB_CMP ali_length=63; seq_ab_match=58; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; merged_sample={'26a_F040644': 1}; forward_score=72.0; score=211.837822462; seq_a_mismatch=4; forward_tag=gaatatc; seq_b_mismatch=1; experiment=wolf_diet; mid_quality=77.7969924812; avg_quality=76.385620915; seq_a_single=45; score_norm=3.36250511845; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=153; reverse_tag=gaatatc; count=1; seq_length=99; mode=alignment; head_quality=67.0; seq_b_single=45; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactacgagcaaca +gcctgaaactcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:30:1800:7833#0/2_CONS_SUB_SUB ali_length=62; seq_ab_match=52; sminR=40.0; tail_quality=65.6; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; merged_sample={'15a_F730814': 1}; forward_score=72.0; score=167.712615339; seq_a_mismatch=0; forward_tag=gaagtag; seq_b_mismatch=10; experiment=wolf_diet; mid_quality=59.9104477612; avg_quality=58.6623376623; seq_a_single=46; score_norm=2.70504218289; status=full; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=gaagtag; count=1; seq_length=100; mode=alignment; head_quality=35.0; seq_b_single=46; +ttagccctaaacacaagacattaatataacgagattaatcgacagagtactaccggctat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:108:9222:18258#0/2_CONS_SUB_SUB ali_length=62; seq_ab_match=60; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; merged_sample={'13a_F730603': 1}; forward_score=72.0; score=231.857703869; seq_a_mismatch=0; forward_tag=aattaac; seq_b_mismatch=2; experiment=wolf_diet; mid_quality=78.5; avg_quality=77.0194805195; seq_a_single=46; score_norm=3.73964038498; status=full; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=aattaac; count=1; seq_length=100; mode=alignment; head_quality=67.2; seq_b_single=46; +ctagccttaaacacaaatagttatgcagacaaaactattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:38:3005:20881#0/2_CONS_SUB_SUB_CMP ali_length=63; seq_ab_match=60; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; merged_sample={'26a_F040644': 1}; forward_score=72.0; score=227.901935995; seq_a_mismatch=3; forward_tag=gaatatc; seq_b_mismatch=0; experiment=wolf_diet; mid_quality=78.5939849624; avg_quality=77.0784313725; seq_a_single=45; score_norm=3.61749104754; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=153; reverse_tag=gaatatc; count=1; seq_length=99; mode=alignment; head_quality=67.0; seq_b_single=45; +ttagccctaaacatgaacattcaataaacaagaatgttcgccagagtactactagcaaca +gcctgaaactcaaaggacttggcggtgctttacatccct diff -r 000000000000 -r aca6a8c9cf83 test-data/output_obiuniq_family.fasta --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/output_obiuniq_family.fasta Wed Apr 12 17:38:00 2017 -0400 @@ -0,0 +1,172 @@ +>HELIUM_000100422_612GNAAXX:7:75:9674:16966#0/2_CONS_SUB_SUB_CMP ali_length=58; sminR=40.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=50; sample=29a_F260619; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; forward_tag=gcctcct; experiment=wolf_diet; seq_a_single=0; merged=['HELIUM_000100422_612GNAAXX:7:75:9674:16966#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:95:11283:2557#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:73:17068:16620#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:96:8737:4973#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:37:12979:6595#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:7:7916:16505#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:13:5742:9859#0/2_CONS_SUB_SUB']; reverse_score=72.0; seq_b_insertion=0; seq_b_deletion=50; seq_a_insertion=0; seq_length_ori=58; reverse_tag=gcctcct; count=7; seq_length=4; status=full; mode=alignment; seq_b_single=0; +tttt +>HELIUM_000100422_612GNAAXX:7:38:3005:20881#0/2_CONS_SUB_SUB_CMP ali_length=63; seq_ab_match=60; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=26a_F040644; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=227.901935995; seq_a_mismatch=3; forward_tag=gaatatc; seq_b_mismatch=0; experiment=wolf_diet; mid_quality=78.5939849624; avg_quality=77.0784313725; seq_a_single=45; score_norm=3.61749104754; merged=['HELIUM_000100422_612GNAAXX:7:38:3005:20881#0/2_CONS_SUB_SUB_CMP']; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=153; reverse_tag=gaatatc; count=1; seq_length=99; mode=alignment; head_quality=67.0; seq_b_single=45; +ttagccctaaacatgaacattcaataaacaagaatgttcgccagagtactactagcaaca +gcctgaaactcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:38:6201:12003#0/2_CONS_SUB_SUB_CMP ali_length=63; seq_ab_match=59; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=26a_F040644; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=219.686655506; seq_a_mismatch=0; forward_tag=gaatatc; seq_b_mismatch=4; experiment=wolf_diet; mid_quality=67.7443609023; avg_quality=67.3986928105; seq_a_single=45; score_norm=3.48708976993; merged=['HELIUM_000100422_612GNAAXX:7:38:6201:12003#0/2_CONS_SUB_SUB_CMP']; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=153; reverse_tag=gaatatc; count=1; seq_length=99; mode=alignment; head_quality=63.2; seq_b_single=45; +ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaata +gcttaaaactcaaagaactgggcggtgctttatatcccg +>HELIUM_000100422_612GNAAXX:7:111:18277:17779#0/2_CONS_SUB_SUB_CMP ali_length=63; seq_ab_match=61; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=26a_F040644; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=235.877819976; seq_a_mismatch=2; forward_tag=gaatatc; seq_b_mismatch=0; experiment=wolf_diet; mid_quality=78.8646616541; avg_quality=77.3137254902; seq_a_single=45; score_norm=3.74409238057; merged=['HELIUM_000100422_612GNAAXX:7:111:18277:17779#0/2_CONS_SUB_SUB_CMP']; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=153; reverse_tag=gaatatc; count=1; seq_length=99; mode=alignment; head_quality=67.0; seq_b_single=45; +ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaata +gcctgaaactcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:78:7151:19928#0/2_CONS_SUB_SUB ali_length=63; seq_ab_match=56; sminR=40.0; tail_quality=47.6; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=26a_F040644; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=195.576462373; seq_a_mismatch=0; forward_tag=gaatatc; seq_b_mismatch=7; experiment=wolf_diet; mid_quality=58.2105263158; avg_quality=57.1699346405; seq_a_single=45; score_norm=3.10438829164; merged=['HELIUM_000100422_612GNAAXX:7:78:7151:19928#0/2_CONS_SUB_SUB']; reverse_score=72.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=153; reverse_tag=gaatatc; count=1; seq_length=99; status=full; mode=alignment; head_quality=52.9; seq_b_single=45; +ttagccctaaacataaacattcaataaacgagaatgttcgccagtgtacttctagcaaca +ggctgaaactcaaaggacttggcggtggtttacatccct +>HELIUM_000100422_612GNAAXX:7:76:10822:18722#0/2_CONS_SUB_SUB ali_length=63; seq_ab_match=58; sminR=40.0; tail_quality=66.8; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=26a_F040644; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=211.83390837; seq_a_mismatch=5; forward_tag=gaatatc; seq_b_mismatch=0; experiment=wolf_diet; mid_quality=77.3834586466; avg_quality=75.9803921569; seq_a_single=45; score_norm=3.36244299; merged=['HELIUM_000100422_612GNAAXX:7:76:10822:18722#0/2_CONS_SUB_SUB']; reverse_score=72.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=153; reverse_tag=gaatatc; count=1; seq_length=99; status=full; mode=alignment; head_quality=66.5; seq_b_single=45; +ttagccctaaacataaacattcaataaacacgaatgttcgccagagtactactagcaaca +gcctgaaactcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:53:6550:1163#0/2_CONS_SUB_SUB ali_length=63; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=26a_F040644; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; forward_tag=gaatatc; seq_b_mismatch=0; experiment=wolf_diet; seq_a_single=45; merged=['HELIUM_000100422_612GNAAXX:7:53:6550:1163#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:38:10813:16867#0/2_CONS_SUB_SUB']; status=full; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=153; reverse_tag=gaatatc; count=2; seq_length=99; mode=alignment; head_quality=67.0; seq_b_single=45; +ttagccctaaacataaacattcaataaacaagaatgtttgccagagtactactagcaaca +gcctgaaactcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:95:1688:9598#0/2_CONS_SUB_SUB_CMP ali_length=63; seq_ab_match=54; sminR=40.0; tail_quality=66.8; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=26a_F040644; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=179.847313607; seq_a_mismatch=6; forward_tag=gaatatc; seq_b_mismatch=3; experiment=wolf_diet; mid_quality=74.2932330827; avg_quality=73.2418300654; seq_a_single=45; score_norm=2.8547192636; merged=['HELIUM_000100422_612GNAAXX:7:95:1688:9598#0/2_CONS_SUB_SUB_CMP']; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=153; reverse_tag=gaatatc; count=1; seq_length=99; mode=alignment; head_quality=65.7; seq_b_single=45; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtgatactagcaaca +gcctgaaactcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:7:11998:4462#0/2_CONS_SUB_SUB ali_length=63; seq_ab_match=59; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=26a_F040644; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=219.864332815; seq_a_mismatch=4; forward_tag=gaatatc; seq_b_mismatch=0; experiment=wolf_diet; mid_quality=78.2105263158; avg_quality=76.7450980392; seq_a_single=45; score_norm=3.48991004469; merged=['HELIUM_000100422_612GNAAXX:7:7:11998:4462#0/2_CONS_SUB_SUB']; reverse_score=72.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=153; reverse_tag=gaatatc; count=1; seq_length=99; status=full; mode=alignment; head_quality=67.0; seq_b_single=45; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtagtactagcaaca +gcctgaaactcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:115:14354:10118#0/2_CONS_SUB_SUB_CMP ali_length=63; seq_ab_match=59; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=26a_F040644; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=219.850378027; seq_a_mismatch=2; forward_tag=gaatatc; seq_b_mismatch=2; experiment=wolf_diet; mid_quality=78.045112782; avg_quality=76.6013071895; seq_a_single=45; score_norm=3.4896885401; merged=['HELIUM_000100422_612GNAAXX:7:115:14354:10118#0/2_CONS_SUB_SUB_CMP']; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=153; reverse_tag=gaatatc; count=1; seq_length=99; mode=alignment; head_quality=67.0; seq_b_single=45; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactcgcaaca +gcctgaaactcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:111:8478:1127#0/2_CONS_SUB_SUB_CMP ali_length=63; seq_ab_match=57; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=26a_F040644; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=203.794981474; seq_a_mismatch=5; forward_tag=gaatatc; seq_b_mismatch=1; experiment=wolf_diet; mid_quality=76.3909774436; avg_quality=75.1241830065; seq_a_single=45; score_norm=3.23484097578; merged=['HELIUM_000100422_612GNAAXX:7:111:8478:1127#0/2_CONS_SUB_SUB_CMP']; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=153; reverse_tag=gaatatc; count=1; seq_length=99; mode=alignment; head_quality=66.4; seq_b_single=45; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca +gcctggaacgcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:16:16357:19948#0/2_CONS_SUB_SUB ali_length=63; seq_ab_match=57; sminR=40.0; tail_quality=66.7; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=26a_F040644; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=203.860719115; seq_a_mismatch=5; forward_tag=gaatatc; seq_b_mismatch=1; experiment=wolf_diet; mid_quality=75.6541353383; avg_quality=74.1764705882; seq_a_single=45; score_norm=3.23588443039; merged=['HELIUM_000100422_612GNAAXX:7:16:16357:19948#0/2_CONS_SUB_SUB']; reverse_score=72.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=153; reverse_tag=gaatatc; count=1; seq_length=99; status=full; mode=alignment; head_quality=62.0; seq_b_single=45; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca +gcctgaaactcacaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:19:6313:16567#0/2_CONS_SUB_SUB_CMP ali_length=63; seq_ab_match=60; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=26a_F040644; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=227.826165596; seq_a_mismatch=3; forward_tag=gaatatc; seq_b_mismatch=0; experiment=wolf_diet; mid_quality=77.4135338346; avg_quality=76.0261437908; seq_a_single=45; score_norm=3.61628834279; merged=['HELIUM_000100422_612GNAAXX:7:19:6313:16567#0/2_CONS_SUB_SUB_CMP']; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=153; reverse_tag=gaatatc; count=1; seq_length=99; mode=alignment; head_quality=66.6; seq_b_single=45; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca +gcctgaaactcaaagggcttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:89:9710:20652#0/2_CONS_SUB_SUB ali_length=63; seq_ab_match=62; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=26a_F040644; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=243.910293117; seq_a_mismatch=0; forward_tag=gaatatc; seq_b_mismatch=1; experiment=wolf_diet; mid_quality=78.8421052632; avg_quality=77.2941176471; seq_a_single=45; score_norm=3.87159195424; merged=['HELIUM_000100422_612GNAAXX:7:89:9710:20652#0/2_CONS_SUB_SUB']; reverse_score=72.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=153; reverse_tag=gaatatc; count=1; seq_length=99; status=full; mode=alignment; head_quality=67.0; seq_b_single=45; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca +gcctgaaactcaaaggacttggcggtgctttatatccct +>HELIUM_000100422_612GNAAXX:7:104:10274:7999#0/2_CONS_SUB_SUB ali_length=63; sminR=40.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=26a_F040644; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; forward_tag=gaatatc; experiment=wolf_diet; seq_a_single=45; merged=['HELIUM_000100422_612GNAAXX:7:104:10274:7999#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:30:16081:4486#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:29:1328:13185#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:4:10139:2389#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:18:11865:6418#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:42:12961:8650#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:38:9654:18700#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:17:11935:17343#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:47:11864:1343#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:21:2093:4412#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:6:6617:2744#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:47:8320:8571#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:115:3396:14235#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:115:6263:17700#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:13:3469:18630#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:18:2721:16829#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:91:10071:8960#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:81:17261:2872#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:86:15907:21191#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:111:15484:19248#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:29:12308:10119#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:99:14794:3606#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:109:17917:15159#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:35:12723:18557#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:10:4085:7492#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:119:2660:19129#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:2:6320:8068#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:102:6364:4627#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:83:18882:9967#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:85:2105:21152#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:32:18486:7018#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:46:13269:4134#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:48:7139:3789#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:42:8435:4155#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:67:7582:3642#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:74:4888:6953#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:95:17941:6113#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:74:8396:18135#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:95:3274:5435#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:111:7660:7465#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:48:5296:16375#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:72:8905:10715#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:4:18261:9148#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:103:8847:6326#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:29:3531:8314#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:49:11740:5547#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:30:5374:18213#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:41:2354:1449#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:111:7441:14688#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:34:18733:3827#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:58:15837:8531#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:117:2416:10342#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:11:8547:18238#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:117:4291:18111#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:69:7696:12881#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:118:13803:15594#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:16:5047:7843#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:117:12470:18434#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:84:11257:18886#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:115:3921:10649#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:109:17335:11429#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:3:14624:12375#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:74:8683:20738#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:57:18459:16145#0/2_CONS_SUB_SUB_CMP']; reverse_score=72.0; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=153; reverse_tag=gaatatc; count=64; seq_length=99; status=full; mode=alignment; seq_b_single=45; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca +gcctgaaactcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:72:12959:5736#0/2_CONS_SUB_SUB_CMP ali_length=63; seq_ab_match=56; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=26a_F040644; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=195.664863172; seq_a_mismatch=0; forward_tag=gaatatc; seq_b_mismatch=7; experiment=wolf_diet; mid_quality=63.5263157895; avg_quality=63.5098039216; seq_a_single=45; score_norm=3.10579147892; merged=['HELIUM_000100422_612GNAAXX:7:72:12959:5736#0/2_CONS_SUB_SUB_CMP']; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=153; reverse_tag=gaatatc; count=1; seq_length=99; mode=alignment; head_quality=59.8; seq_b_single=45; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca +gcctgaaactcaaaggacttggcggtgctttacacccct +>HELIUM_000100422_612GNAAXX:7:81:15726:2345#0/2_CONS_SUB_SUB_CMP ali_length=63; seq_ab_match=56; sminR=40.0; tail_quality=62.3; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=26a_F040644; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=195.641193686; seq_a_mismatch=0; forward_tag=gaatatc; seq_b_mismatch=7; experiment=wolf_diet; mid_quality=63.1954887218; avg_quality=63.091503268; seq_a_single=45; score_norm=3.10541577279; merged=['HELIUM_000100422_612GNAAXX:7:81:15726:2345#0/2_CONS_SUB_SUB_CMP']; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=153; reverse_tag=gaatatc; count=1; seq_length=99; mode=alignment; head_quality=62.5; seq_b_single=45; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca +gcctgaaactcaaagcactcggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:120:16005:7955#0/2_CONS_SUB_SUB_CMP ali_length=64; seq_ab_match=61; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=26a_F040644; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=231.880428082; seq_a_mismatch=1; forward_tag=gaatatc; seq_b_mismatch=2; experiment=wolf_diet; mid_quality=78.8939393939; avg_quality=77.3289473684; seq_a_single=44; score_norm=3.62313168878; merged=['HELIUM_000100422_612GNAAXX:7:120:16005:7955#0/2_CONS_SUB_SUB_CMP']; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=152; reverse_tag=gaatatc; count=1; seq_length=98; mode=alignment; head_quality=67.0; seq_b_single=44; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca +gccgaaactcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:19:4311:13343#0/2_CONS_SUB_SUB_CMP ali_length=63; seq_ab_match=58; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=26a_F040644; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=211.837822462; seq_a_mismatch=4; forward_tag=gaatatc; seq_b_mismatch=1; experiment=wolf_diet; mid_quality=77.7969924812; avg_quality=76.385620915; seq_a_single=45; score_norm=3.36250511845; merged=['HELIUM_000100422_612GNAAXX:7:19:4311:13343#0/2_CONS_SUB_SUB_CMP']; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=153; reverse_tag=gaatatc; count=1; seq_length=99; mode=alignment; head_quality=67.0; seq_b_single=45; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactacgagcaaca +gcctgaaactcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:13:6954:13039#0/2_CONS_SUB_SUB_CMP ali_length=63; seq_ab_match=62; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=26a_F040644; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=243.872474161; seq_a_mismatch=1; forward_tag=gaatatc; seq_b_mismatch=0; experiment=wolf_diet; mid_quality=79.0; avg_quality=77.431372549; seq_a_single=45; score_norm=3.87099165336; merged=['HELIUM_000100422_612GNAAXX:7:13:6954:13039#0/2_CONS_SUB_SUB_CMP']; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=153; reverse_tag=gaatatc; count=1; seq_length=99; mode=alignment; head_quality=67.0; seq_b_single=45; +ttagccctaaacataaacattcaataaacaagaatgttcgccagaggactactagcaata +gcttaaaactcaaaggacttggcggtgctttatatccct +>HELIUM_000100422_612GNAAXX:7:116:14244:17926#0/2_CONS_SUB_SUB ali_length=63; seq_ab_match=55; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=26a_F040644; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=187.882758912; seq_a_mismatch=5; forward_tag=gaatatc; seq_b_mismatch=3; experiment=wolf_diet; mid_quality=76.5338345865; avg_quality=75.2875816993; seq_a_single=45; score_norm=2.98226601448; merged=['HELIUM_000100422_612GNAAXX:7:116:14244:17926#0/2_CONS_SUB_SUB']; reverse_score=72.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=153; reverse_tag=gaatatc; count=1; seq_length=99; status=full; mode=alignment; head_quality=67.0; seq_b_single=45; +ttagccctaaacataaacattcaataaacaagaatgttcgcaagagtactactagcaaca +gcctgaaactcaaaggacttggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:7:15538:8325#0/2_CONS_SUB_SUB_CMP ali_length=63; seq_ab_match=52; sminR=40.0; tail_quality=63.4; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=26a_F040644; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=151.662770749; seq_a_mismatch=0; forward_tag=gaatatc; seq_b_mismatch=10; experiment=wolf_diet; mid_quality=60.1879699248; avg_quality=60.3333333333; seq_a_single=45; score_norm=2.40734556744; merged=['HELIUM_000100422_612GNAAXX:7:7:15538:8325#0/2_CONS_SUB_SUB_CMP']; status=full; direction=reverse; seq_b_insertion=1; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=153; reverse_tag=gaatatc; count=1; seq_length=99; mode=alignment; head_quality=59.2; seq_b_single=45; +ttagccctaaacataaacattcaataaacaagaatgtcggccagagtactactagcaaca +gcatgaaactcaaagaactgggcggtgctttacatccct +>HELIUM_000100422_612GNAAXX:7:29:18416:16620#0/2_CONS_SUB_SUB_CMP ali_length=62; seq_ab_match=60; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=29a_F260619; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=231.873033258; seq_a_mismatch=2; forward_tag=gcctcct; seq_b_mismatch=0; experiment=wolf_diet; mid_quality=77.4179104478; avg_quality=76.0649350649; seq_a_single=46; score_norm=3.7398876332; merged=['HELIUM_000100422_612GNAAXX:7:29:18416:16620#0/2_CONS_SUB_SUB_CMP']; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=gcctcct; count=1; seq_length=100; mode=alignment; head_quality=67.0; seq_b_single=46; +ttagccctaaacacaagtaattattataacaaaattattcgccagagttctaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctg +>HELIUM_000100422_612GNAAXX:7:46:9246:19171#0/2_CONS_SUB_SUB ali_length=62; sminR=40.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=29a_F260619; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; forward_tag=gcctcct; experiment=wolf_diet; seq_a_single=46; merged=['HELIUM_000100422_612GNAAXX:7:46:9246:19171#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:29:15520:18035#0/2_CONS_SUB_SUB']; status=full; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=gcctcct; count=2; seq_length=100; mode=alignment; seq_b_single=46; +ttagccctaaacacaagtaattattataacaaaattattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:119:14871:19157#0/2_CONS_SUB_SUB_CMP ali_length=62; seq_ab_match=47; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=29a_F260619; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=115.761290673; seq_a_mismatch=1; forward_tag=gcctcct; seq_b_mismatch=13; experiment=wolf_diet; mid_quality=69.1492537313; avg_quality=68.8701298701; seq_a_single=46; score_norm=1.86711759151; merged=['HELIUM_000100422_612GNAAXX:7:119:14871:19157#0/2_CONS_SUB_SUB_CMP']; status=full; direction=reverse; seq_b_insertion=1; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=154; reverse_tag=gcctcct; count=1; seq_length=100; mode=alignment; head_quality=67.0; seq_b_single=46; +ttagccctaaacacaagtaattattataacaaaatcattcgccagagtactaccggcaat +agctcaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:42:19757:3800#0/2_CONS_SUB_SUB_CMP ali_length=62; seq_ab_match=57; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=1; sample=15a_F730814; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=187.78301321; seq_a_mismatch=1; forward_tag=gaagtag; seq_b_mismatch=3; experiment=wolf_diet; mid_quality=77.7669172932; avg_quality=76.3660130719; seq_a_single=45; score_norm=3.02875827758; merged=['HELIUM_000100422_612GNAAXX:7:42:19757:3800#0/2_CONS_SUB_SUB_CMP']; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=1; seq_a_insertion=1; seq_length_ori=153; reverse_tag=gaagtag; count=1; seq_length=99; mode=alignment; head_quality=67.1; seq_b_single=46; +ttagccctaaacacaagtaattaatatacaaaattattcgccagagtactaccggcaata +gcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:108:5640:3823#0/2_CONS_SUB_SUB_CMP ali_length=62; seq_ab_match=60; sminR=40.0; tail_quality=66.9; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=29a_F260619; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=231.881364714; seq_a_mismatch=0; forward_tag=gcctcct; seq_b_mismatch=2; experiment=wolf_diet; mid_quality=78.447761194; avg_quality=76.9545454545; seq_a_single=46; score_norm=3.74002201152; merged=['HELIUM_000100422_612GNAAXX:7:108:5640:3823#0/2_CONS_SUB_SUB_CMP']; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=gcctcct; count=1; seq_length=100; mode=alignment; head_quality=67.0; seq_b_single=46; +ttagccctaaacacaagtaattaatataacaaaattgttcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:59:11971:12063#0/2_CONS_SUB_SUB ali_length=62; seq_ab_match=62; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=29a_F260619; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=247.995051728; seq_a_mismatch=0; forward_tag=gcctcct; seq_b_mismatch=0; experiment=wolf_diet; mid_quality=78.9850746269; avg_quality=77.4285714286; seq_a_single=46; score_norm=3.99992018915; merged=['HELIUM_000100422_612GNAAXX:7:59:11971:12063#0/2_CONS_SUB_SUB']; reverse_score=72.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=gcctcct; count=1; seq_length=100; status=full; mode=alignment; head_quality=67.0; seq_b_single=46; +ttagccctaaacacaagtaattaatataacaaaattattcgtcagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:76:8491:8900#0/2_CONS_SUB_SUB_CMP ali_length=62; seq_ab_match=57; sminR=40.0; tail_quality=65.3; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=15a_F730814; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=207.828655885; seq_a_mismatch=4; forward_tag=gaagtag; seq_b_mismatch=1; experiment=wolf_diet; mid_quality=76.552238806; avg_quality=75.1948051948; seq_a_single=46; score_norm=3.35207509492; merged=['HELIUM_000100422_612GNAAXX:7:76:8491:8900#0/2_CONS_SUB_SUB_CMP']; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=gaagtag; count=1; seq_length=100; mode=alignment; head_quality=66.9; seq_b_single=46; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtgctacctgcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:114:4511:17596#0/2_CONS_SUB_SUB_CMP ali_length=62; seq_ab_match=57; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=29a_F260619; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=207.883730153; seq_a_mismatch=4; forward_tag=gcctcct; seq_b_mismatch=1; experiment=wolf_diet; mid_quality=75.3208955224; avg_quality=74.2532467532; seq_a_single=46; score_norm=3.35296338956; merged=['HELIUM_000100422_612GNAAXX:7:114:4511:17596#0/2_CONS_SUB_SUB_CMP']; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=gcctcct; count=1; seq_length=100; mode=alignment; head_quality=67.2; seq_b_single=46; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtgctaccggcaat +agcttaaaactcaaaggacttggcggtgctttatacgctt +>HELIUM_000100422_612GNAAXX:7:7:11110:17924#0/2_CONS_SUB_SUB ali_length=62; seq_ab_match=58; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=15a_F730814; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=215.812113201; seq_a_mismatch=3; forward_tag=gaagtag; seq_b_mismatch=1; experiment=wolf_diet; mid_quality=75.723880597; avg_quality=74.5519480519; seq_a_single=46; score_norm=3.4808405355; merged=['HELIUM_000100422_612GNAAXX:7:7:11110:17924#0/2_CONS_SUB_SUB']; reverse_score=72.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=gaagtag; count=1; seq_length=100; status=full; mode=alignment; head_quality=66.4; seq_b_single=46; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtcataccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:3:17077:6562#0/2_CONS_SUB_SUB_CMP ali_length=62; seq_ab_match=59; sminR=40.0; tail_quality=66.6; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=15a_F730814; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=223.876050008; seq_a_mismatch=3; forward_tag=gaagtag; seq_b_mismatch=0; experiment=wolf_diet; mid_quality=77.6567164179; avg_quality=76.1818181818; seq_a_single=46; score_norm=3.61090403239; merged=['HELIUM_000100422_612GNAAXX:7:3:17077:6562#0/2_CONS_SUB_SUB_CMP']; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=gaagtag; count=1; seq_length=100; mode=alignment; head_quality=66.0; seq_b_single=46; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactgccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:120:1900:13295#0/2_CONS_SUB_SUB_CMP ali_length=62; seq_ab_match=60; sminR=40.0; tail_quality=67.1; reverse_match=tagaacaggctcctctag; seq_a_deletion=1; sample=15a_F730814; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=219.874676069; seq_a_mismatch=1; forward_tag=gaagtag; seq_b_mismatch=0; experiment=wolf_diet; mid_quality=77.5149253731; avg_quality=76.1558441558; seq_a_single=46; score_norm=3.54636574304; merged=['HELIUM_000100422_612GNAAXX:7:120:1900:13295#0/2_CONS_SUB_SUB_CMP']; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=1; seq_length_ori=154; reverse_tag=gaagtag; count=1; seq_length=100; mode=alignment; head_quality=67.0; seq_b_single=46; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactacgcgcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:14:3978:11028#0/2_CONS_SUB_SUB_CMP ali_length=62; sminR=40.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; seq_b_mismatch=0; experiment=wolf_diet; seq_a_single=46; merged=['HELIUM_000100422_612GNAAXX:7:14:3978:11028#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:115:17402:4174#0/2_CONS_SUB_SUB_CMP']; reverse_score=72.0; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; count=2; seq_length=100; status=full; mode=alignment; seq_b_single=46; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactacctgcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:71:17473:7401#0/2_CONS_SUB_SUB_CMP ali_length=62; seq_ab_match=60; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=15a_F730814; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=231.883938289; seq_a_mismatch=1; forward_tag=gaagtag; seq_b_mismatch=1; experiment=wolf_diet; mid_quality=77.2089552239; avg_quality=75.7857142857; seq_a_single=46; score_norm=3.74006352079; merged=['HELIUM_000100422_612GNAAXX:7:71:17473:7401#0/2_CONS_SUB_SUB_CMP']; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=gaagtag; count=1; seq_length=100; mode=alignment; head_quality=65.5; seq_b_single=46; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggtaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:24:16230:12703#0/2_CONS_SUB_SUB_CMP ali_length=62; seq_ab_match=59; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=15a_F730814; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=223.86908915; seq_a_mismatch=2; forward_tag=gaagtag; seq_b_mismatch=1; experiment=wolf_diet; mid_quality=77.4552238806; avg_quality=76.0519480519; seq_a_single=46; score_norm=3.61079176049; merged=['HELIUM_000100422_612GNAAXX:7:24:16230:12703#0/2_CONS_SUB_SUB_CMP']; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=gaagtag; count=1; seq_length=100; mode=alignment; head_quality=66.3; seq_b_single=46; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttatcccctt +>HELIUM_000100422_612GNAAXX:7:13:17353:13234#0/2_CONS_SUB_SUB_CMP ali_length=62; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=15a_F730814; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; seq_a_mismatch=1; forward_tag=gaagtag; experiment=wolf_diet; seq_a_single=46; merged=['HELIUM_000100422_612GNAAXX:7:13:17353:13234#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:70:11798:2668#0/2_CONS_SUB_SUB']; status=full; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=gaagtag; count=2; seq_length=100; mode=alignment; seq_b_single=46; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccttt +>HELIUM_000100422_612GNAAXX:7:14:7706:12338#0/2_CONS_SUB_SUB_CMP sminR=40.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; experiment=wolf_diet; merged=['HELIUM_000100422_612GNAAXX:7:14:7706:12338#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:59:15367:8376#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:74:4783:20479#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:16:19299:8684#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:66:11860:20419#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:36:10388:10324#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:101:11720:19906#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:67:5288:13403#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:17:16877:9760#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:53:2128:13141#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:7:17828:13825#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:58:8591:17028#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:2:7296:17275#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:61:17484:5085#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:19:11039:3957#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:16:15509:18069#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:99:13467:17714#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:1:14576:14181#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:25:11691:21134#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:74:8297:20108#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:54:2542:19180#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:82:6036:6921#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:46:11654:6037#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:15:16118:15111#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:28:9406:18226#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:63:4725:12502#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:113:2918:18736#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:33:9296:13526#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:53:10153:11845#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:73:16525:10764#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:83:6795:14061#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:41:16707:19819#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:38:13721:2004#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:99:14742:5591#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:41:19763:3556#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:40:3610:8896#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:44:19605:15509#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:93:18556:10479#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:96:19317:7551#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:110:17877:11976#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:108:2729:8600#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:114:10615:11217#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:94:12367:17729#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:85:18986:17662#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:86:10092:15563#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:65:6787:8456#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:68:19115:18847#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:71:14800:3222#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:52:10700:16508#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:53:14379:5922#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:78:18994:16521#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:11:8428:13716#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:35:3798:1896#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:72:13060:5051#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:109:12848:8882#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:120:14963:1929#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:108:17701:18887#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:50:2791:11138#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:89:10621:20317#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:111:2893:6772#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:22:8540:14708#0/2_CONS_SUB_SUB']; status=full; seq_length_ori=154; count=61; seq_length=100; mode=alignment; seq_b_single=46; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:107:3570:18147#0/2_CONS_SUB_SUB_CMP ali_length=62; seq_ab_match=60; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=29a_F260619; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=231.924768153; seq_a_mismatch=2; forward_tag=gcctcct; seq_b_mismatch=0; experiment=wolf_diet; mid_quality=78.0820895522; avg_quality=76.5714285714; seq_a_single=46; score_norm=3.74072206698; merged=['HELIUM_000100422_612GNAAXX:7:107:3570:18147#0/2_CONS_SUB_SUB_CMP']; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=gcctcct; count=1; seq_length=100; mode=alignment; head_quality=65.9; seq_b_single=46; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat +agcataaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:97:14311:19299#0/2_CONS_SUB_SUB_CMP ali_length=62; seq_ab_match=52; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=29a_F260619; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=167.91734192; seq_a_mismatch=5; forward_tag=gcctcct; seq_b_mismatch=5; experiment=wolf_diet; mid_quality=75.0820895522; avg_quality=74.0064935065; seq_a_single=46; score_norm=2.70834422451; merged=['HELIUM_000100422_612GNAAXX:7:97:14311:19299#0/2_CONS_SUB_SUB_CMP']; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=gcctcct; count=1; seq_length=100; mode=alignment; head_quality=66.6; seq_b_single=46; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaag +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:25:7789:3090#0/2_CONS_SUB_SUB_CMP ali_length=62; seq_ab_match=59; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=29a_F260619; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=223.705772044; seq_a_mismatch=0; forward_tag=gcctcct; seq_b_mismatch=3; experiment=wolf_diet; mid_quality=72.4179104478; avg_quality=71.6818181818; seq_a_single=46; score_norm=3.60815761361; merged=['HELIUM_000100422_612GNAAXX:7:25:7789:3090#0/2_CONS_SUB_SUB_CMP']; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=gcctcct; count=1; seq_length=100; mode=alignment; head_quality=66.5; seq_b_single=46; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactacaggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:105:14135:6905#0/2_CONS_SUB_SUB ali_length=62; seq_ab_match=61; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=29a_F260619; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=239.985379811; seq_a_mismatch=1; forward_tag=gcctcct; seq_b_mismatch=0; experiment=wolf_diet; mid_quality=78.8134328358; avg_quality=77.2792207792; seq_a_single=46; score_norm=3.87073193244; merged=['HELIUM_000100422_612GNAAXX:7:105:14135:6905#0/2_CONS_SUB_SUB']; reverse_score=72.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=gcctcct; count=1; seq_length=100; status=full; mode=alignment; head_quality=67.0; seq_b_single=46; +ttagccctaaacacaagtaattaatataacaaaattattcaccagagtactagcggcaac +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:111:19168:18517#0/2_CONS_SUB_SUB ali_length=63; seq_ab_match=60; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=15a_F730814; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=223.825469474; seq_a_mismatch=1; forward_tag=gaagtag; seq_b_mismatch=1; experiment=wolf_diet; mid_quality=77.8955223881; avg_quality=76.4805194805; seq_a_single=45; score_norm=3.55278522975; merged=['HELIUM_000100422_612GNAAXX:7:111:19168:18517#0/2_CONS_SUB_SUB']; reverse_score=72.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=1; seq_length_ori=154; reverse_tag=gaagtag; count=1; seq_length=100; status=full; mode=alignment; head_quality=67.0; seq_b_single=46; +ttagccctaaacacaagtaattaatataacaaaattatccgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:10:9237:10532#0/2_CONS_SUB_SUB_CMP ali_length=62; seq_ab_match=57; sminR=40.0; tail_quality=66.9; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=29a_F260619; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=207.90944986; seq_a_mismatch=2; forward_tag=gcctcct; seq_b_mismatch=3; experiment=wolf_diet; mid_quality=75.9402985075; avg_quality=74.7532467532; seq_a_single=46; score_norm=3.35337822355; merged=['HELIUM_000100422_612GNAAXX:7:10:9237:10532#0/2_CONS_SUB_SUB_CMP']; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=gcctcct; count=1; seq_length=100; mode=alignment; head_quality=66.7; seq_b_single=46; +ttagccctaaacacaagtaattaatataacaaaataattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:30:1800:7833#0/2_CONS_SUB_SUB ali_length=62; seq_ab_match=52; sminR=40.0; tail_quality=65.6; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=15a_F730814; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=167.712615339; seq_a_mismatch=0; forward_tag=gaagtag; seq_b_mismatch=10; experiment=wolf_diet; mid_quality=59.9104477612; avg_quality=58.6623376623; seq_a_single=46; score_norm=2.70504218289; merged=['HELIUM_000100422_612GNAAXX:7:30:1800:7833#0/2_CONS_SUB_SUB']; reverse_score=72.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=gaagtag; count=1; seq_length=100; status=full; mode=alignment; head_quality=35.0; seq_b_single=46; +ttagccctaaacacaagacattaatataacgagattaatcgacagagtactaccggctat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:41:9316:2256#0/2_CONS_SUB_SUB ali_length=62; seq_ab_match=61; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=29a_F260619; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=239.993848494; seq_a_mismatch=1; forward_tag=gcctcct; seq_b_mismatch=0; experiment=wolf_diet; mid_quality=78.6343283582; avg_quality=77.1233766234; seq_a_single=46; score_norm=3.87086852409; merged=['HELIUM_000100422_612GNAAXX:7:41:9316:2256#0/2_CONS_SUB_SUB']; reverse_score=72.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=gcctcct; count=1; seq_length=100; status=full; mode=alignment; head_quality=67.0; seq_b_single=46; +ttagccctaaacacaagaaattaatataacaaaattattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:85:13257:14594#0/2_CONS_SUB_SUB_CMP ali_length=62; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=29a_F260619; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; seq_a_mismatch=2; forward_tag=gcctcct; experiment=wolf_diet; seq_a_single=46; merged=['HELIUM_000100422_612GNAAXX:7:85:13257:14594#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:14:15397:17819#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:89:10281:5930#0/2_CONS_SUB_SUB_CMP']; reverse_score=72.0; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=gcctcct; count=3; seq_length=100; status=full; mode=alignment; seq_b_single=46; +ttagccctaaacacaaataattacacaaacaaaattgttcaccagagtactagcggcaac +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:76:18268:14980#0/2_CONS_SUB_SUB_CMP ali_length=62; seq_ab_match=61; sminR=40.0; tail_quality=66.9; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=29a_F260619; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=239.985254914; seq_a_mismatch=1; forward_tag=gcctcct; seq_b_mismatch=0; experiment=wolf_diet; mid_quality=78.3208955224; avg_quality=76.8441558442; seq_a_single=46; score_norm=3.87072991797; merged=['HELIUM_000100422_612GNAAXX:7:76:18268:14980#0/2_CONS_SUB_SUB_CMP']; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=gcctcct; count=1; seq_length=100; mode=alignment; head_quality=67.0; seq_b_single=46; +ttacccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:108:9222:18258#0/2_CONS_SUB_SUB ali_length=62; seq_ab_match=60; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=13a_F730603; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=231.857703869; seq_a_mismatch=0; forward_tag=aattaac; seq_b_mismatch=2; experiment=wolf_diet; mid_quality=78.5; avg_quality=77.0194805195; seq_a_single=46; score_norm=3.73964038498; merged=['HELIUM_000100422_612GNAAXX:7:108:9222:18258#0/2_CONS_SUB_SUB']; reverse_score=72.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=aattaac; count=1; seq_length=100; status=full; mode=alignment; head_quality=67.2; seq_b_single=46; +ctagccttaaacacaaatagttatgcagacaaaactattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:103:3594:15996#0/2_CONS_SUB_SUB ali_length=62; seq_ab_match=60; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=13a_F730603; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=231.92194325; seq_a_mismatch=2; forward_tag=aattaac; seq_b_mismatch=0; experiment=wolf_diet; mid_quality=78.3805970149; avg_quality=76.9090909091; seq_a_single=46; score_norm=3.74067650404; merged=['HELIUM_000100422_612GNAAXX:7:103:3594:15996#0/2_CONS_SUB_SUB']; reverse_score=72.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=aattaac; count=1; seq_length=100; status=full; mode=alignment; head_quality=67.1; seq_b_single=46; +ctagccttaaacacaaatagttatgcaaacacaactattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:22:1798:3790#0/2_CONS_SUB_SUB_CMP ali_length=62; seq_ab_match=59; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=13a_F730603; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=223.82253574; seq_a_mismatch=3; forward_tag=aattaac; seq_b_mismatch=0; experiment=wolf_diet; mid_quality=78.3208955224; avg_quality=76.8506493506; seq_a_single=46; score_norm=3.61004089903; merged=['HELIUM_000100422_612GNAAXX:7:22:1798:3790#0/2_CONS_SUB_SUB_CMP']; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=aattaac; count=1; seq_length=100; mode=alignment; head_quality=67.0; seq_b_single=46; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtgctaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:55:16528:11418#0/2_CONS_SUB_SUB ali_length=62; seq_ab_match=58; sminR=40.0; tail_quality=57.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=13a_F730603; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=215.988419267; seq_a_mismatch=4; forward_tag=aattaac; seq_b_mismatch=0; experiment=wolf_diet; mid_quality=76.0746268657; avg_quality=74.2142857143; seq_a_single=46; score_norm=3.48368418172; merged=['HELIUM_000100422_612GNAAXX:7:55:16528:11418#0/2_CONS_SUB_SUB']; reverse_score=72.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=aattaac; count=1; seq_length=100; status=full; mode=alignment; head_quality=66.5; seq_b_single=46; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcact +agcttacaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:54:14618:14353#0/2_CONS_SUB_SUB_CMP sminR=40.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=13a_F730603; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; forward_tag=aattaac; experiment=wolf_diet; merged=['HELIUM_000100422_612GNAAXX:7:54:14618:14353#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:116:12598:9964#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:43:8022:8427#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:88:15797:19191#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:18:5240:1587#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:12:9609:14740#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:16:16055:12835#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:72:7057:20448#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:32:15197:9940#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:53:18419:8404#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:89:16663:15081#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:43:7980:7908#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:63:18851:7688#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:77:18518:10758#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:86:11013:15234#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:59:18371:8149#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:23:13304:5167#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:97:10495:17046#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:120:4726:20808#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:100:17253:5513#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:46:1592:11866#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:58:18618:20959#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:13:14386:20412#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:114:8865:19510#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:89:16162:8889#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:21:5487:19582#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:47:3878:6141#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:78:10338:12302#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:47:7619:9866#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:97:9416:9155#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:32:18262:6966#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:82:8836:11354#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:16:4418:5128#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:71:18821:20471#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:45:6831:6228#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:32:4360:3451#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:33:16506:1490#0/2_CONS_SUB_SUB', 'HELIUM_000100422_612GNAAXX:7:21:4713:10229#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:44:12484:5320#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:19:4865:20715#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:7:10646:8487#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:18:5317:11571#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:12:17693:3451#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:97:9091:15775#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:97:10414:9929#0/2_CONS_SUB_SUB_CMP', 'HELIUM_000100422_612GNAAXX:7:6:9274:14951#0/2_CONS_SUB_SUB']; reverse_score=72.0; seq_b_deletion=0; seq_a_insertion=0; reverse_tag=aattaac; count=46; seq_length=100; status=full; mode=alignment; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:99:8183:13912#0/2_CONS_SUB_SUB ali_length=62; seq_ab_match=60; sminR=40.0; tail_quality=67.1; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=13a_F730603; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=231.903850642; seq_a_mismatch=1; forward_tag=aattaac; seq_b_mismatch=1; experiment=wolf_diet; mid_quality=78.3358208955; avg_quality=76.8701298701; seq_a_single=46; score_norm=3.74038468777; merged=['HELIUM_000100422_612GNAAXX:7:99:8183:13912#0/2_CONS_SUB_SUB']; reverse_score=72.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=aattaac; count=1; seq_length=100; status=full; mode=alignment; head_quality=67.0; seq_b_single=46; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctc +>HELIUM_000100422_612GNAAXX:7:4:4214:9434#0/2_CONS_SUB_SUB ali_length=62; seq_ab_match=58; sminR=40.0; tail_quality=63.3; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=13a_F730603; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=215.812703412; seq_a_mismatch=3; forward_tag=aattaac; seq_b_mismatch=1; experiment=wolf_diet; mid_quality=76.2388059701; avg_quality=74.6883116883; seq_a_single=46; score_norm=3.48085005502; merged=['HELIUM_000100422_612GNAAXX:7:4:4214:9434#0/2_CONS_SUB_SUB']; reverse_score=72.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=aattaac; count=1; seq_length=100; status=full; mode=alignment; head_quality=65.3; seq_b_single=46; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactacaggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:9:10358:4089#0/2_CONS_SUB_SUB_CMP ali_length=61; seq_ab_match=54; sminR=40.0; tail_quality=66.9; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=13a_F730603; reverse_score=72.0; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=181.620394786; seq_a_mismatch=1; forward_tag=aattaac; seq_b_mismatch=6; experiment=wolf_diet; mid_quality=66.3880597015; avg_quality=66.4545454545; seq_a_single=46; score_norm=2.97738352108; merged=['HELIUM_000100422_612GNAAXX:7:9:10358:4089#0/2_CONS_SUB_SUB_CMP']; status=full; direction=reverse; seq_b_insertion=0; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=154; reverse_tag=aattaac; count=1; seq_length=100; mode=alignment; head_quality=66.9; seq_b_single=47; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactacaggcaat +agctcaaaactcaaagaacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:33:9900:9541#0/2_CONS_SUB_SUB ali_length=62; seq_ab_match=62; sminR=40.0; tail_quality=67.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=0; sample=13a_F730603; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=247.949713348; seq_a_mismatch=0; forward_tag=aattaac; seq_b_mismatch=0; experiment=wolf_diet; mid_quality=78.8955223881; avg_quality=77.3506493506; seq_a_single=46; score_norm=3.99918892497; merged=['HELIUM_000100422_612GNAAXX:7:33:9900:9541#0/2_CONS_SUB_SUB']; reverse_score=72.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=154; reverse_tag=aattaac; count=1; seq_length=100; status=full; mode=alignment; head_quality=67.0; seq_b_single=46; +ctagccctaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat +agcttaaaactcaaaggacttggcggtgctttataccctt +>HELIUM_000100422_612GNAAXX:7:115:3625:10051#0/2_CONS_SUB_SUB ali_length=59; seq_ab_match=59; sminR=40.0; tail_quality=93.0; reverse_match=tagaacaggctcctctag; seq_a_deletion=49; sample=13a_F730603; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_primer=tagaacaggctcctctag; sminL=40.0; forward_score=72.0; score=235.999642017; seq_a_mismatch=0; forward_tag=aattaac; seq_b_mismatch=0; experiment=wolf_diet; mid_quality=93.0; avg_quality=93.0; seq_a_single=0; score_norm=3.99999393249; merged=['HELIUM_000100422_612GNAAXX:7:115:3625:10051#0/2_CONS_SUB_SUB']; reverse_score=72.0; direction=forward; seq_b_insertion=0; seq_b_deletion=49; seq_a_insertion=0; seq_length_ori=59; reverse_tag=aattaac; count=1; seq_length=5; status=full; mode=alignment; head_quality=93.0; seq_b_single=0; +caata diff -r 000000000000 -r aca6a8c9cf83 test-data/wolf_small.F.fastq --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/wolf_small.F.fastq Wed Apr 12 17:38:00 2017 -0400 @@ -0,0 +1,998 @@ +@HELIUM_000100422_612GNAAXX:7:119:14871:19157#0/1 +ccgcctcctttagataccccactatgcttagccctaaacacaagtaattattataacaaaatcattcgccagagtactaccggcaatagctcaaaactcaaagaactt ++ +bbbbbbbbbbbbbbbbbbbbbbabbbba_abbbbbbbbbbbbbaZbbb`bbbbbbb``b`QXK^[TWXXJ`\`BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HELIUM_000100422_612GNAAXX:7:108:5640:3823#0/1 +ccgcctcctttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattgttcgccagagtactaccggcaatagcttaaaactcaaaggactt ++ +bbbbbbbabbbbbbbbbbbbbbbbbbbbbbabbbbbabbbbbbb[bbbb`bbZb^bbbb_`Z\KaZXRYZbb\bZ\\^^BBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HELIUM_000100422_612GNAAXX:7:97:14311:19299#0/1 +ccgcctcctttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccgccactagcttaaaactcaaagaactc ++ +bbbbbbbbbbbbbbbbbbbbbbbaabbb^abbbbbab^bbbb__Z`````Tb_b_bbb_b]]Zb_Vbb_bXRZRPYYT[[``[_BBBBBBBBBBBBBBBBBBBBBBBB +@HELIUM_000100422_612GNAAXX:7:22:8540:14708#0/1 +ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaat ++ +bbbbbbbbbabbbbbbbbabbbbbbbbbbb`\```_bbbbbbbbbbb^b`bbb^bb_b_b`bb^b`\a^O`Z```UZ]R^`b^b^W```R[[LW[a`b_QQ[U^BBBB +@HELIUM_000100422_612GNAAXX:7:57:18459:16145#0/1 +ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaccagcctgaaactcacaggactcg ++ +bbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbcbbba^b^b``bb`Z`Z`^^^W^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HELIUM_000100422_612GNAAXX:7:89:10281:5930#0/1 +ccgcctcctttagataccccactatgcttagccctaaacacaaataattacacaaacaaaattgttcaccagagtactagcggcaacagcttaaaactcaaagtactc ++ +bbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbabbbbbbabbbbbbbb`bb_bbbbbaaa`a```^\UXW[XU`[W`BBBBBBBBBBBBBBBBBBBBBBBBBBBBB +@HELIUM_000100422_612GNAAXX:7:29:15520:18035#0/1 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