Mercurial > repos > iuc > pairtools_split
changeset 8:0fb51c6f2e37 draft default tip
planemo upload for repository https://github.com/open2c/pairtools commit 365c6f6387b2f90af22a62e673873b90e7f7394a
| author | iuc |
|---|---|
| date | Fri, 25 Jul 2025 13:54:22 +0000 |
| parents | f4b5f58b89bf |
| children | |
| files | test-data/output_parsed_pairs_sam_mapq.pairs |
| diffstat | 1 files changed, 600 insertions(+), 0 deletions(-) [+] |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/output_parsed_pairs_sam_mapq.pairs Fri Jul 25 13:54:22 2025 +0000 @@ -0,0 +1,600 @@ +## pairs format v1.0.0 +#shape: upper triangle +#genome_assembly: unknown +#chromsize: Test_seq 450060 +#samheader: @SQ SN:Test_seq LN:450060 +#samheader: @PG ID:bwa-mem2 PN:bwa-mem2 VN:2.2.1 CL:bwa-mem2 mem -5SP -T0 -t 8 test test_hic_reads_1.fq test_hic_reads_2.fq +#samheader: @PG ID:pairtools_parse PN:pairtools_parse CL:/usr/local/bin/pairtools parse /tmp/tmpu2wbua_3/files/c/8/8/dataset_c88eeb08-5a4e-41bc-84ac-fe34bb7e42f9.dat -c /tmp/tmpu2wbua_3/files/2/8/b/dataset_28bb6843-f06e-45f9-b608-6c75c7408162.dat -o /tmp/tmpu2wbua_3/job_working_directory/000/31/outputs/dataset_0a810c2e-9b60-4852-b164-7a4b14ffcdac.dat --min-mapq 1 --max-molecule-size 750 --add-columns mapq,seq --walks-policy mask --max-inter-align-gap 20 --nproc-in 1 --nproc-out 1 PP:bwa-mem2 VN:1.1.3 +#columns: readID chrom1 pos1 chrom2 pos2 strand1 strand2 pair_type sam1 sam2 mapq1 mapq2 seq1 seq2 +A00126:224:H7HMWDSX3:3:1101:4779:3098 Test_seq 166211 Test_seq 214901 - - UU A00126:224:H7HMWDSX3:3:1101:4779:3098113Test_seq1661344378M=21482448691GGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFNM:i:0MD:Z:78MC:Z:78MAS:i:78XS:i:63XA:Z:Test_seq,+214829,5S73M,2;Yt:Z:UU A00126:224:H7HMWDSX3:3:1101:4779:3098177Test_seq214824578M=166134-48691AAGAGCCCAAGAGACAGAAAGGGCCACATGAACCACAGACCTACATCATCCTGAGACCAGAAGAACTAGTTGGTGCCCFFFF,FFFFFFFFFFFFF,FFFFFFFFFFFF:FF:,FFFFFFF:FF:FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFNM:i:3MD:Z:1T33G33A8MC:Z:78MAS:i:66XS:i:63XA:Z:Test_seq,+166134,78M,3;Yt:Z:UU 43 5 GGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCTT AAGAGCCCAAGAGACAGAAAGGGCCACATGAACCACAGACCTACATCATCCTGAGACCAGAAGAACTAGTTGGTGCCC +A00126:224:H7HMWDSX3:3:1101:2908:3239 Test_seq 167331 Test_seq 167696 + - UU A00126:224:H7HMWDSX3:3:1101:2908:3239161Test_seq16733123151M=167546366TGAGATGTCATAGCTGAATATTCTTTCCCAGTCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFF:FFFFF:FFFF:FFFFFFFFFFFFFFF:FF,,FFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFF:FFFFFFFF:FF,FFNM:i:1MD:Z:37A113MC:Z:151MAS:i:146XS:i:135XA:Z:Test_seq,-213553,151M,4;Yt:Z:UU A00126:224:H7HMWDSX3:3:1101:2908:323981Test_seq16754644151M=167331-366TAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTACAAATGGATATCCAGGTATGCCAGCACCATTTGTTAAAAAGACTATTATTTCCCCAATTGACTGACACFF,F,F:FFFFFFFFF,FFF:FFFFFF:FFFF:FFFFFFFFFFF:FF::F,FFFFFFFFF:,F,FFF:FFFFFFFFFFFFFFFFFFFFF:FF:FFF::FFFFFFFFFFFFF,FFFFFFF:FF:F,:FFFFFFFFFF,F,F:FFFFFFFFFFNM:i:3MD:Z:82G15T30C21MC:Z:151MAS:i:136XS:i:114XA:Z:Test_seq,+213342,118M2I23M2I6M,8;Yt:Z:UU 23 44 TGAGATGTCATAGCTGAATATTCTTTCCCAGTCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTG TAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTACAAATGGATATCCAGGTATGCCAGCACCATTTGTTAAAAAGACTATTATTTCCCCAATTGACTGACAC +A00126:224:H7HMWDSX3:3:1101:27208:4492 Test_seq 194620 Test_seq 194909 + - UU A00126:224:H7HMWDSX3:3:1101:27208:449297Test_seq19462060151M=194778290GCTGTGCAAACCACTGCTCCAGGCTATCTCAGCACTGAGCCAGAGACAAGGGCCAGTTCAGTCACCTGGAAAATCAGGCCAGCATCTCTGAAAAATAGGTGCCTCAGTGTTCACAGAAAAGGGCCATGTATTGAAGTAGGTCAGAGGAAGAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFF:FFFFFFFFF:F,FF:::FFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:132M19SAS:i:151XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1101:27208:4492145Test_seq19477860132M19S=194620-290CACTCACCAGATTCAGCCCAGGACCAGAGGCTGCCGAGGGCAGGTGTGGGACGAAGTCAAGATGAAGTCAGTTCATTGTGTGGTTTTCGGTACCAGGCTAGACCTAGATTTGAACCCCAAGTCTGCTACTTAGATCATTCATTCAATGTGTFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFFF:FFFFFFFFF:FFFFF:FFFFFFFFF:FFFFFF:FFFFFFFFF:FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:132MC:Z:151MAS:i:132XS:i:0Yt:Z:UU 60 60 GCTGTGCAAACCACTGCTCCAGGCTATCTCAGCACTGAGCCAGAGACAAGGGCCAGTTCAGTCACCTGGAAAATCAGGCCAGCATCTCTGAAAAATAGGTGCCTCAGTGTTCACAGAAAAGGGCCATGTATTGAAGTAGGTCAGAGGAAGA CACTCACCAGATTCAGCCCAGGACCAGAGGCTGCCGAGGGCAGGTGTGGGACGAAGTCAAGATGAAGTCAGTTCATTGTGTGGTTTTCGGTACCAGGCTAGACCTAGATTTGAACCCCAAGTCTGCTACTTAGATCATTCATTCAATGTGT +A00126:224:H7HMWDSX3:3:1101:9344:5306 Test_seq 449503 Test_seq 449562 + - UU A00126:224:H7HMWDSX3:3:1101:9344:530697Test_seq449503760M=44950360GTTCGGACAGAACAAGGGGATACTGATTGGTTTAAAGTCAGGAAAGGTATGAGTCAGTGT,FFFF,FFF:FF,FF:FF:FF:F:,F::,FF:,FFFFFFFF::FF,,:FFF,FFF::,F,NM:i:4MD:Z:3T44G2C5G2MC:Z:60MAS:i:44XS:i:40XA:Z:Test_seq,+16926,60M,5;Yt:Z:UU A00126:224:H7HMWDSX3:3:1101:9344:5306145Test_seq4495031260M=449503-60GTTTGGACAGAACAAGGGGATACTGATTGGTTTAAAGTCAGGAAAGGTGTGCGCCAGGGTFFFFFFFFF:F::FF,FFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFF:F:FFFFNM:i:1MD:Z:53T6MC:Z:60MAS:i:55XS:i:50XA:Z:Test_seq,-16926,60M,2;Yt:Z:UU 7 12 GTTCGGACAGAACAAGGGGATACTGATTGGTTTAAAGTCAGGAAAGGTATGAGTCAGTGT GTTTGGACAGAACAAGGGGATACTGATTGGTTTAAAGTCAGGAAAGGTGTGCGCCAGGGT +A00126:224:H7HMWDSX3:3:1101:17607:5212 Test_seq 83425 Test_seq 93852 - + UR A00126:224:H7HMWDSX3:3:1101:17607:52122129Test_seq9415560122M29H=93852-425GCAATGAAGCAGCAGTTTTTGTAGTGAAGGGAAATGTCCTGATAACATTAAGTAAGAAATGAACTACAGGCCAGGGCATTGGATTGATTTAGTGTGGAGTTTCTCCAGCAATTTGTCAGATC:FF:FFF:FFFF,FFFFFFFFFFFFFFFFF:FFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:122MC:Z:151MAS:i:122XS:i:0SA:Z:Test_seq,83397,-,122S29M,60,0;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1101:17607:521281Test_seq8339760122S29M=9385210428GCAATGAAGCAGCAGTTTTTGTAGTGAAGGGAAATGTCCTGATAACATTAAGTAAGAAATGAACTACAGGCCAGGGCATTGGATTGATTTAGTGTGGAGTTTCTCCAGCAATTTGTCAGATCAATCCAGCTGATTTTTGTGTGTTTACCTT:FF:FFF:FFFF,FFFFFFFFFFFFFFFFF:FFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFNM:i:0MD:Z:29MC:Z:151MAS:i:29XS:i:0SA:Z:Test_seq,94155,-,122M29S,60,0;Yt:Z:UR A00126:224:H7HMWDSX3:3:1101:17607:5212161Test_seq9385260151M=83397-10428AAACATGGAACGGCAAAAAATGTGGCACCGACCCGGCTATATGAAGGACACTTGTTTACAGTGTGAGAGCTGAAACAAGAAGGCAGAGTGCGGCTTTGTTTGACCTCTGTTGGAAACGCGTGCATCGGGTGATTCAAAATTTCCACCACTCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:122S29MAS:i:151XS:i:0Yt:Z:UR 60 60 GCAATGAAGCAGCAGTTTTTGTAGTGAAGGGAAATGTCCTGATAACATTAAGTAAGAAATGAACTACAGGCCAGGGCATTGGATTGATTTAGTGTGGAGTTTCTCCAGCAATTTGTCAGATCAATCCAGCTGATTTTTGTGTGTTTACCTT AAACATGGAACGGCAAAAAATGTGGCACCGACCCGGCTATATGAAGGACACTTGTTTACAGTGTGAGAGCTGAAACAAGAAGGCAGAGTGCGGCTTTGTTTGACCTCTGTTGGAAACGCGTGCATCGGGTGATTCAAAATTTCCACCACTC +A00126:224:H7HMWDSX3:3:1101:17942:7263 Test_seq 168528 Test_seq 168867 + - UU A00126:224:H7HMWDSX3:3:1101:17942:726397Test_seq16852860151M=168717340ATTTAGAGTGATGTTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTGAGAGTTTTTATCATAAATGGGTGCTGGACTTTGTCAAATGCCTTTTCTGCATCAATTGATAAGATCATGFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF,F,F:FFFFFF,FFFFFF,FFFFFFFFFFFFFFFFFFFFFF:FF:FFFFF:FFFFF:FFFF,FFFFFFFFF:FFFFF,::FFFF::F:FFFNM:i:5MD:Z:12A68A23T2A26T15MC:Z:151MAS:i:126XS:i:91Yt:Z:UU A00126:224:H7HMWDSX3:3:1101:17942:7263145Test_seq16871760151M=168528-340ATTAATGGTTTTTCTGATATTAAACCAACCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTCTATTGGCTAGAATTTTGTTGAGGATTTTTGCATCTATGTTCATGAGGGATATAGGTFFFFFFF::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:27G123MC:Z:151MAS:i:146XS:i:91Yt:Z:UU 60 60 ATTTAGAGTGATGTTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTGAGAGTTTTTATCATAAATGGGTGCTGGACTTTGTCAAATGCCTTTTCTGCATCAATTGATAAGATCATG ATTAATGGTTTTTCTGATATTAAACCAACCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTCTATTGGCTAGAATTTTGTTGAGGATTTTTGCATCTATGTTCATGAGGGATATAGGT +A00126:224:H7HMWDSX3:3:1101:5683:8108 ! 0 Test_seq 170834 - + MR A00126:224:H7HMWDSX3:3:1101:5683:810865Test_seq137032028M123S=17083433803GATTTTTATTTTCTTTATGTTGAGGTGTGATCGATCCAAAAAGAAAAACACCAAGACATATTATCATCAAACTTGCCAAAACCAAAGACAAACAGAAAATTTTAAAAGCAGCCAGGGAGAAAAGAAAGGTTTCCTTCAAGGGAGAATCAATFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFF:FFFFFNM:i:1MD:Z:7G20MC:Z:151MAS:i:23XS:i:23SA:Z:Test_seq,171170,-,119M32S,60,0;XA:Z:Test_seq,+333108,28M123S,1;Test_seq,-298750,123S26M2S,1;Test_seq,-279319,123S28M,2;Yt:Z:MRNEXT_SAMA00126:224:H7HMWDSX3:3:1101:5683:81082129Test_seq17117060119M32H=170834-455ATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCFFFFF:FFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:FFFFFFFFFNM:i:0MD:Z:119MC:Z:151MAS:i:119XS:i:0SA:Z:Test_seq,137032,+,28M123S,0,1;Yt:Z:MR A00126:224:H7HMWDSX3:3:1101:5683:8108129Test_seq17083460151M=137032-33803ATCTTACTTTGTTTGTTTTTGTGCTTTCCCTATTTGAGTTGCGTTGATATCAGGACGTTCTGTTTTGTGACCTTGTATTGTGCTGGTACCTGATATTATTGGTCATCAGGCCAAACAATCTCCTTAAGCATTTCTTGCAGTCTTGGTTTAGFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF::FFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:28M123SAS:i:151XS:i:0Yt:Z:MR 0 60 GATTTTTATTTTCTTTATGTTGAGGTGTGATCGATCCAAAAAGAAAAACACCAAGACATATTATCATCAAACTTGCCAAAACCAAAGACAAACAGAAAATTTTAAAAGCAGCCAGGGAGAAAAGAAAGGTTTCCTTCAAGGGAGAATCAAT ATCTTACTTTGTTTGTTTTTGTGCTTTCCCTATTTGAGTTGCGTTGATATCAGGACGTTCTGTTTTGTGACCTTGTATTGTGCTGGTACCTGATATTATTGGTCATCAGGCCAAACAATCTCCTTAAGCATTTCTTGCAGTCTTGGTTTAG +A00126:224:H7HMWDSX3:3:1101:3947:9361 ! 0 Test_seq 171174 - + NR A00126:224:H7HMWDSX3:3:1101:3947:9361145Test_seq17134260129M22S=171174-297TGATGTCAGGGAAGTTTTGTGTCAGGAGTTCTTCAACTATTTTCTCTGTGTTTTCTGTCCCCCCTCCCTGTTCTGGGACTCCAATCACTCGCAAGTTATCCTTCTTGATAGAGTCCCACATGATTCTTATAAAAATAAAATGCTTGCTTACFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:FFFFFNM:i:2MD:Z:58G15T54MC:Z:151MAS:i:119XS:i:0Yt:Z:NR A00126:224:H7HMWDSX3:3:1101:3947:936197Test_seq17117460151M=171342297ATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATGGGGTTCGATGAGCATCTTGGATAGAFFFFFFFFFFFFFFFF,,:FF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF,FFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FF:FFF:F:FFFFFFFF::FFFFFF:FFFFFFFF:FFFFF,FFFFFFFFFF,FFFFFFF:FFF,FNM:i:0MD:Z:151MC:Z:129M22SAS:i:151XS:i:37Yt:Z:NR 0 60 ATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATGGGGTTCGATGAGCATCTTGGATAGA +A00126:224:H7HMWDSX3:3:1101:6596:9721 Test_seq 213805 Test_seq 213859 + - UU A00126:224:H7HMWDSX3:3:1101:6596:972197Test_seq2138051155M=21380555CTTCACTAAAGAAGATATTCAGGCAGCTAACAGATACATGAGGAAATGCTCTCGAFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF,FFFFFF:FF:F:FFFF:FF:NM:i:1MD:Z:42A12MC:Z:55MAS:i:50XS:i:45XA:Z:Test_seq,+41478,55M,2;Test_seq,-167175,55M,3;Yt:Z:UU A00126:224:H7HMWDSX3:3:1101:6596:9721145Test_seq2138051655M=213805-55CTTCACTAAAGAAGATATTCAGGCAGCTAACAGATACATGAGAAAATGCTCCCGAFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFFFNM:i:1MD:Z:51T3MC:Z:55MAS:i:51XS:i:45XA:Z:Test_seq,+167175,55M,3;Yt:Z:UU 11 16 CTTCACTAAAGAAGATATTCAGGCAGCTAACAGATACATGAGGAAATGCTCTCGA CTTCACTAAAGAAGATATTCAGGCAGCTAACAGATACATGAGAAAATGCTCCCGA +A00126:224:H7HMWDSX3:3:1101:1777:10175 ! 0 Test_seq 62699 - + NR A00126:224:H7HMWDSX3:3:1101:1777:1017581Test_seq6287560126M25S=62699-302TTGTGCTAAGAAAATAATTGTCATTGACCTTAACGAAACCCCTTATTATTTCCAAAACAAAAGCATAATATTGAACCAGCTCTTTTGAAGAGATTTAAATACACTGCATCATTGGCAAAGGGGACTTAATAGTTTTGTGTGTAGGTCTCCTFF,F,,FFF,FFFFFF:FFF::FF::F,F:FFFFFFFF,,,,:F,FFFFFF,:FFF,,FFFF:F,FFF::,,:FF,FF:F:FFFF:FFF,,F:FF:FFFFF:FFFFFFF::FF:,:FFFF:,FF:FFFF,F,,FFFFFFFFFFF,FFFFFFNM:i:4MD:Z:44C11C7T10G50MC:Z:151MAS:i:106XS:i:0Yt:Z:NR A00126:224:H7HMWDSX3:3:1101:1777:10175161Test_seq6269960151M=62875302CGGGACAAAGACATCATCGGAGGTCTGCAATGCCGACTGGATGGCCTCGCTTCCCTCTCACCTCCACAACGTCCCCCTTTCCAATCTGGCAATACCAGGTATTCTTCTCTTCCTCCTTGTTCCTACTGTTTAGTTCTGCCATCTTAGCGTTFFFFF,FFFF:FFFFFF,FFFFFFF,FFFFFFFFF:FFFFFFF,:FFFFFFF,F:FFFFFFF::FF:FFF:FF,FFFF:FF:FFF::FFFFFFF,:F:FFFFFFFFF:FFFFF:F,FFFFF,,FF:FFFFFF,:FF:F,:FFFF:FFF,,FNM:i:0MD:Z:151MC:Z:126M25SAS:i:151XS:i:0Yt:Z:NR 0 60 CGGGACAAAGACATCATCGGAGGTCTGCAATGCCGACTGGATGGCCTCGCTTCCCTCTCACCTCCACAACGTCCCCCTTTCCAATCTGGCAATACCAGGTATTCTTCTCTTCCTCCTTGTTCCTACTGTTTAGTTCTGCCATCTTAGCGTT +A00126:224:H7HMWDSX3:3:1101:15845:10269 ! 0 Test_seq 167871 - + MU A00126:224:H7HMWDSX3:3:1101:15845:1026981Test_seq1681290151M=167871-409TACATCCTTGGTAAGATTTATTCCTAAGTATTTTATCTTCTTGGGGGCTACTGGGAATGCTATTGATTTGGTTATTTCCTCTTCGGTGTTCTTCTTGTTGATGTAGAGGAATCCAAGTGATTTTTGTACGTTTATTTTATAACCTGAGACT:FFFFFF:FFFFFF:FF:FFFFFFFFF:FFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF::FFFFFFFF:FFNM:i:8MD:Z:3C5C30C12T5G33T34T13T8MC:Z:151MAS:i:112XS:i:111XA:Z:Test_seq,+212759,151M,8;Yt:Z:MU A00126:224:H7HMWDSX3:3:1101:15845:10269161Test_seq16787160151M=168129409CTTTTTCAGTAATGCTTTGCTTATCCGGGGGTTCTTTCCCTTCCATATGAAATTAGTGATTTGTTTCTCTATCCCCTTAAAATATGACATTGGTATTTGGATTGGAAGTGCGTTATATGTATAGATGGCTTTTGGTAGAATAGACATTTTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:NM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:MU 0 60 TACATCCTTGGTAAGATTTATTCCTAAGTATTTTATCTTCTTGGGGGCTACTGGGAATGCTATTGATTTGGTTATTTCCTCTTCGGTGTTCTTCTTGTTGATGTAGAGGAATCCAAGTGATTTTTGTACGTTTATTTTATAACCTGAGACT CTTTTTCAGTAATGCTTTGCTTATCCGGGGGTTCTTTCCCTTCCATATGAAATTAGTGATTTGTTTCTCTATCCCCTTAAAATATGACATTGGTATTTGGATTGGAAGTGCGTTATATGTATAGATGGCTTTTGGTAGAATAGACATTTTT +A00126:224:H7HMWDSX3:3:1101:18412:10802 Test_seq 167532 Test_seq 213099 + + UU A00126:224:H7HMWDSX3:3:1101:18412:10802129Test_seq16753252151M=21309945568GATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATCTTTTCCCFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFF:FFFFFFFFFFFFFF:FFFFFFFF:F:::F:FFF:,F:F:::,,NM:i:1MD:Z:144A6MC:Z:9S142MAS:i:146XS:i:121XA:Z:Test_seq,-213356,104M2I23M2I20M,6;Yt:Z:UU A00126:224:H7HMWDSX3:3:1101:18412:1080265Test_seq213099279S142M=167532-45568AAGGTGAGAGAGAAACAAATCACCAACTTCATATGGAAGGGAAAGAAGCTTCGGATAAGCAAAGCATTACTGAAAAAGAAGAAGAAAGTGGGAGGCCTCACTCTACCTGATTTCAGAACCTCTTATACAGCCACAGTAGTCAAAACAGCCTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:5MD:Z:9G30C9T61A9T19MC:Z:151MAS:i:117XS:i:103XA:Z:Test_seq,-167798,151M,10;Yt:Z:UU 52 27 GATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATCTTTTCCC AAGGTGAGAGAGAAACAAATCACCAACTTCATATGGAAGGGAAAGAAGCTTCGGATAAGCAAAGCATTACTGAAAAAGAAGAAGAAAGTGGGAGGCCTCACTCTACCTGATTTCAGAACCTCTTATACAGCCACAGTAGTCAAAACAGCCT +A00126:224:H7HMWDSX3:3:1101:18421:11287 Test_seq 214825 Test_seq 214876 + - UU A00126:224:H7HMWDSX3:3:1101:18421:1128797Test_seq2148253152M=21482952TGAGCCCAAGAGACAGAAAGGGCCACATGAACCAGAGACCTACATCATCCTGFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:52MC:Z:4S48MAS:i:52XS:i:43XA:Z:Test_seq,-166159,48M4S,1;Yt:Z:UU A00126:224:H7HMWDSX3:3:1101:18421:11287145Test_seq21482934S48M=214825-52AGAACCCAAGAGACAGAGAGGGCCACATGAACCAGAGACCTACATCATCCTG,FF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:13A34MC:Z:52MAS:i:43XS:i:42XA:Z:Test_seq,+166159,52M,2;Yt:Z:UU 31 3 TGAGCCCAAGAGACAGAAAGGGCCACATGAACCAGAGACCTACATCATCCTG AGAACCCAAGAGACAGAGAGGGCCACATGAACCAGAGACCTACATCATCCTG +A00126:224:H7HMWDSX3:3:1101:18801:13416 Test_seq 63815 Test_seq 64265 + - UU A00126:224:H7HMWDSX3:3:1101:18801:1341697Test_seq6381560151M=64115451TTTTGGAGATCTTCCAGGTGCATTGTCCCCATGGGCCCTGGTTGCTTTTAAGCAAGGAAAGGGCTGCTGAATTTCTTTATGGTTATGTCTACTTGTGTTTTATTAGCTGTTGGCTACAGCTTGCTGGGTAATGCCCGATGGCTATGCTTCAFFFFFFFFF:FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFF:FF:FFFFFFFFFFF:FF,:F:,FFFFF:F:FFFFFFFFFFFFFFFFFFFF,FFFFFFF:FFFFFFFFFFFFFFFFFF,FFFNM:i:1MD:Z:87A63MC:Z:151MAS:i:146XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1101:18801:13416145Test_seq6411560151M=63815-451AGAATGTCATCTTAGGCAGGCCCTATATGGCTCTCTCCTGGGCATGTTCAGTGTAGGTTGGGGCTTCCTCTTTTCTTTCTGTGCTTTGGACGGCTTAGACTGTAACAGCAGCTCTGCCAGTGGCTTGGCTGTGTATTCTGACTCTGTGACT:FFFFFFFFFFFFFF,FFF:FFF,FF:FFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFF:FFFFFFFF,FFF:F:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU 60 60 TTTTGGAGATCTTCCAGGTGCATTGTCCCCATGGGCCCTGGTTGCTTTTAAGCAAGGAAAGGGCTGCTGAATTTCTTTATGGTTATGTCTACTTGTGTTTTATTAGCTGTTGGCTACAGCTTGCTGGGTAATGCCCGATGGCTATGCTTCA AGAATGTCATCTTAGGCAGGCCCTATATGGCTCTCTCCTGGGCATGTTCAGTGTAGGTTGGGGCTTCCTCTTTTCTTTCTGTGCTTTGGACGGCTTAGACTGTAACAGCAGCTCTGCCAGTGGCTTGGCTGTGTATTCTGACTCTGTGACT +A00126:224:H7HMWDSX3:3:1101:27263:14137 Test_seq 213158 Test_seq 213255 + - UU A00126:224:H7HMWDSX3:3:1101:27263:14137161Test_seq213158998M=21315898ACTGAAAAAGAAGAAGAAAGTGGGAGGCCTCACTCTACCTGATTTCAGAACCTATTATACAGCCACAGTAGTCAAAACAGCCTGGTACTGGTACAACAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFNM:i:1MD:Z:63T34MC:Z:98MAS:i:93XS:i:88XA:Z:Test_seq,-167783,98M,2;Test_seq,-152052,98M,5;Yt:Z:UU A00126:224:H7HMWDSX3:3:1101:27263:1413781Test_seq213158998M=213158-98ACTGAAAAAGAAGAAGAAAGTGGGAGGCCTCACTCTACCTGATTTCAGAACCTATTATACAGCCACAGTAGTCAAAACAGCCTGGTACTGGTACAACAFFFFFFFFFFFFFFFFFF,FFFF:FF:FFFFFFFFFFFFFFFFFF:FFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFNM:i:1MD:Z:63T34MC:Z:98MAS:i:93XS:i:88XA:Z:Test_seq,+167783,98M,2;Test_seq,+152052,98M,5;Yt:Z:UU 9 9 ACTGAAAAAGAAGAAGAAAGTGGGAGGCCTCACTCTACCTGATTTCAGAACCTATTATACAGCCACAGTAGTCAAAACAGCCTGGTACTGGTACAACA ACTGAAAAAGAAGAAGAAAGTGGGAGGCCTCACTCTACCTGATTTCAGAACCTATTATACAGCCACAGTAGTCAAAACAGCCTGGTACTGGTACAACA +A00126:224:H7HMWDSX3:3:1101:20645:14137 Test_seq 170038 Test_seq 170424 + - UU A00126:224:H7HMWDSX3:3:1101:20645:14137161Test_seq17003860151M=170274387ATTGTGGAGGTGTCTATCTCGCTTTTCAATTCTGTTAAAATTTGATTTATGTATCTTGCAGCCCTGTCATTGAGTGCGTAAACATTTAATATGGTTATATCTTCCTGATCAATTGTCCCTTTTATCATTATATAGTGTCCTTCTTTATCCTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFF:FFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFNM:i:5MD:Z:1A7A59C2G9T68MC:Z:151MAS:i:129XS:i:81Yt:Z:UU A00126:224:H7HMWDSX3:3:1101:20645:1413781Test_seq17027460151M=170038-387TATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATTGTGTTTCTTTATCCAGTCCGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFNM:i:1MD:Z:83C67MC:Z:151MAS:i:146XS:i:80Yt:Z:UU 60 60 ATTGTGGAGGTGTCTATCTCGCTTTTCAATTCTGTTAAAATTTGATTTATGTATCTTGCAGCCCTGTCATTGAGTGCGTAAACATTTAATATGGTTATATCTTCCTGATCAATTGTCCCTTTTATCATTATATAGTGTCCTTCTTTATCCT TATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATTGTGTTTCTTTATCCAGTCCGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGC +A00126:224:H7HMWDSX3:3:1101:6054:16297 Test_seq 169279 Test_seq 169573 + - UU A00126:224:H7HMWDSX3:3:1101:6054:1629797Test_seq16927960151M=169423295CTTATTCGGGTTATTTGTTTCCTTTCCTGTATTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF,FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF,FFFFFFF:FFFFFFFFF,,FFFNM:i:1MD:Z:30T120MC:Z:151MAS:i:146XS:i:103Yt:Z:UU A00126:224:H7HMWDSX3:3:1101:6054:16297145Test_seq16942332151M=169279-295TCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGATTCTTTTGTTGCTCACTTTCTATTTGTTCAAGTTGTAGGGACAGTTCTCTGATTTTGGCTCTTTCTTCTTTTTGTATGTGTGCATTTATTGATATAAATTGACCTCFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:7MD:Z:44G36C0A13C0C36C11G4MC:Z:151MAS:i:116XS:i:98XA:Z:Test_seq,+39146,151M,11;Yt:Z:UU 60 32 CTTATTCGGGTTATTTGTTTCCTTTCCTGTATTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTC TCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGATTCTTTTGTTGCTCACTTTCTATTTGTTCAAGTTGTAGGGACAGTTCTCTGATTTTGGCTCTTTCTTCTTTTTGTATGTGTGCATTTATTGATATAAATTGACCTC +A00126:224:H7HMWDSX3:3:1101:11225:16767 ! 0 Test_seq 41043 - - MU A00126:224:H7HMWDSX3:3:1101:11225:16767177Test_seq167664058M=40986-126679AAAAAGACTATTATTTCCCCAATTGACTGACACTGGTCCTTTGTCAAATATCAGCTGCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFNM:i:1MD:Z:11C46MC:Z:58MAS:i:53XS:i:53XA:Z:Test_seq,+213317,58M,1;Test_seq,+40986,58M,2;Yt:Z:MU A00126:224:H7HMWDSX3:3:1101:11225:16767113Test_seq409864058M=167664126679GCAGCTGATATTTGACAAAGGCCCAGTGTCAGTTAATTGGGGAAAAGATAGTCTTTTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:2MD:Z:21A23T12MC:Z:58MAS:i:48XS:i:34Yt:Z:MU 0 40 AAAAAGACTATTATTTCCCCAATTGACTGACACTGGTCCTTTGTCAAATATCAGCTGC GCAGCTGATATTTGACAAAGGCCCAGTGTCAGTTAATTGGGGAAAAGATAGTCTTTTT +A00126:224:H7HMWDSX3:3:1101:6352:16814 Test_seq 118685 Test_seq 119060 + - UU A00126:224:H7HMWDSX3:3:1101:6352:1681497Test_seq11868560151M=118910376CCAGTGATACAATGAAAGATATTTGTTAAAGATAATTTTCACCTTAAAATAGTTTTAATTGGAATGTTTTTCTTATTGTAGTTAGAAATGTGTCTATTGTGACCAGTCATACTTTATGACAATAAGGTAATCTCAGTGTGTAGGAGGATCGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFF:FFFFFFFNM:i:2MD:Z:139C10C0MC:Z:151MAS:i:145XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1101:6352:16814145Test_seq11891060151M=118685-376GATATGAGTTCAGAAAACCAAACCAAAACCAAACCCATTGCCGTCAAGTCGATCCCGACTTGTGGAGACTGTATAGGACAGAGTAGAACTGCCCCAGAAGGTTCCCAGTTTTTATGGGAGCATATTGCCACATCTTCTCCCAAAGAGTGGC:FFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:25Yt:Z:UU 60 60 CCAGTGATACAATGAAAGATATTTGTTAAAGATAATTTTCACCTTAAAATAGTTTTAATTGGAATGTTTTTCTTATTGTAGTTAGAAATGTGTCTATTGTGACCAGTCATACTTTATGACAATAAGGTAATCTCAGTGTGTAGGAGGATCG GATATGAGTTCAGAAAACCAAACCAAAACCAAACCCATTGCCGTCAAGTCGATCCCGACTTGTGGAGACTGTATAGGACAGAGTAGAACTGCCCCAGAAGGTTCCCAGTTTTTATGGGAGCATATTGCCACATCTTCTCCCAAAGAGTGGC +A00126:224:H7HMWDSX3:3:1101:3088:17143 Test_seq 218095 Test_seq 218439 + - UU A00126:224:H7HMWDSX3:3:1101:3088:17143161Test_seq21809560151M=218307345ACCAGTTGCTGCCAAGTCATTTCCATCTCATGGCAACCCCAGTTATGTCAGATTAGAACCTCATACCATAGGAATTTTAATGGCTTGTGACCTTTCAGAAATAGATCACCAGGTCTTTCTTCTAAGGTACGTCTGTGTGGACTCGAACTGCF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFF:FFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFFFF:FFFFFFFF:FFFFFFF:FFFFFF:FFFFFFF:FFFFFFFFFFF,FFFF:,FFFFFFFFFFFFFFFFFFFFF,:FNM:i:0MD:Z:151MC:Z:133M18SAS:i:151XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1101:3088:1714381Test_seq21830760133M18S=218095-345ACTCTATTGCAAAGCACTGTTCTTCCAGCTCTACTGCCATCTCATGTCAGCCTTCCAACAACCTTATAAGGTATATACTATCATTGTTAACATCATTTTCATTTTACGGATGAAACTGAGGAGTAGAGAGATCTAACTTTTTATTTCAAAGFFF:FF:FFFFFFFFFFF:::FF:FFFFFFFFFFFFFFF,FF,:FFFFFFF:F,FFF,,F,,FFFFF,,::FF,FFF:FF:FFFFFFFFFFFFFFF,FFF:FFFFFFF:FFFFFFFFFF:,FFFFFFFF:FFF:FFF::FFFFFFF,,:F:NM:i:1MD:Z:73G59MC:Z:151MAS:i:128XS:i:0Yt:Z:UU 60 60 ACCAGTTGCTGCCAAGTCATTTCCATCTCATGGCAACCCCAGTTATGTCAGATTAGAACCTCATACCATAGGAATTTTAATGGCTTGTGACCTTTCAGAAATAGATCACCAGGTCTTTCTTCTAAGGTACGTCTGTGTGGACTCGAACTGC ACTCTATTGCAAAGCACTGTTCTTCCAGCTCTACTGCCATCTCATGTCAGCCTTCCAACAACCTTATAAGGTATATACTATCATTGTTAACATCATTTTCATTTTACGGATGAAACTGAGGAGTAGAGAGATCTAACTTTTTATTTCAAAG +A00126:224:H7HMWDSX3:3:1101:9760:17957 Test_seq 112944 Test_seq 113304 + - UU A00126:224:H7HMWDSX3:3:1101:9760:17957161Test_seq11294460151M=113172361GAACTGCTCACCTTTTGGCTAGCAGCCAAGTTCTTAACTACCACGCCACAAGGGCTCCTTCATGCCAAGGTAGTGCACCTTAAAAGAAAAAAGGATCTGTGTCCTCAAGAAACCTGTGGTCTCTAGCGGTAAAAGAAAGGAAGAGAAAATAF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFF,FFFFF:FFFFFFFF,FFFFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFF:FFFFNM:i:0MD:Z:151MC:Z:133M18SAS:i:151XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1101:9760:1795781Test_seq11317260133M18S=112944-361ATGGGTTTTCAAAAGGGGCTGAGAATCAGGGAATCAGGAAGGACTTCATAGGGAAGGTGATATTTAATATGGGTTATGAGGGCTGGGACAAAGATGTAAACAGGGTGAGAGAATGTCTCACAGGAAGGTGTTAGATCTTGGCTGAAAGCAAFFFFFFFF:FFFF,F::FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFNM:i:0MD:Z:133MC:Z:151MAS:i:133XS:i:0Yt:Z:UU 60 60 GAACTGCTCACCTTTTGGCTAGCAGCCAAGTTCTTAACTACCACGCCACAAGGGCTCCTTCATGCCAAGGTAGTGCACCTTAAAAGAAAAAAGGATCTGTGTCCTCAAGAAACCTGTGGTCTCTAGCGGTAAAAGAAAGGAAGAGAAAATA ATGGGTTTTCAAAAGGGGCTGAGAATCAGGGAATCAGGAAGGACTTCATAGGGAAGGTGATATTTAATATGGGTTATGAGGGCTGGGACAAAGATGTAAACAGGGTGAGAGAATGTCTCACAGGAAGGTGTTAGATCTTGGCTGAAAGCAA +A00126:224:H7HMWDSX3:3:1101:16342:18458 Test_seq 214062 Test_seq 214362 + - UU A00126:224:H7HMWDSX3:3:1101:16342:18458161Test_seq21406260151M=214212301AAATAGAACTACCATACAACCCAGAAATCCCACTCCTCGGAATATACCCTAGAGAAATAAGAGCCTTCACACAAACAGATATATGCACACCCATGTTTATTGCAGCTCTGTTTACAATAGCAAAAAGATGGAAGCAACCAAGGTGTCCATC::FF:FFFFFFFFFFFFFFF:FFF::FFF:FF,FFFFFF,::FFFFF:FFFFFF,FFFF,F:F,FFFFFF,F:F,F:,F:FFFFFFF::FF,FFF,FFFF:FF:F:FFFFFFFFFFFFFFFF:F:,F,:FF,FF:F:F::FFFFF::,FFFNM:i:1MD:Z:127C23MC:Z:151MAS:i:146XS:i:108Yt:Z:UU A00126:224:H7HMWDSX3:3:1101:16342:1845881Test_seq21421234151M=214062-301CAATGGATGAATGTTTAAATAAATAGTGGTATATTCACACAATGGAATACTACGCATCGATAAAGAACAGTGACAAATCTGTGAAACATTTCATAACATGGAGGAACCTGGAAGGCATTATGCTGAGCGAAATTGGTCAGAGGCAAAAGGA::F:FFFFFFF,F,:::FFFF:FFFFF,F:FFFFFFF,::FFF,FF:F:FFF:FFFFFFFFFFFFF:FFFFF,,FFFFF:FF,FFF,FFF:F,F:F:FFFF:FF:FFFF:FFF,,F:FFFF,FF:FF,::FFFFF:,FFF,F:FFFFFFFFNM:i:7MD:Z:13G10T27T5A15G52T6A16MC:Z:151MAS:i:116XS:i:97XA:Z:Test_seq,+166672,151M,11;Yt:Z:UU 60 34 AAATAGAACTACCATACAACCCAGAAATCCCACTCCTCGGAATATACCCTAGAGAAATAAGAGCCTTCACACAAACAGATATATGCACACCCATGTTTATTGCAGCTCTGTTTACAATAGCAAAAAGATGGAAGCAACCAAGGTGTCCATC CAATGGATGAATGTTTAAATAAATAGTGGTATATTCACACAATGGAATACTACGCATCGATAAAGAACAGTGACAAATCTGTGAAACATTTCATAACATGGAGGAACCTGGAAGGCATTATGCTGAGCGAAATTGGTCAGAGGCAAAAGGA +A00126:224:H7HMWDSX3:3:1101:25048:19601 ! 0 Test_seq 374646 - + NR A00126:224:H7HMWDSX3:3:1101:25048:19601145Test_seq37489160127M24S=374646-372ACTTCTTTTTTTTTGGTATGTTCTTTCTTTTACATTTCAGAGTTGCTGGCTTCTTCAGCTCCAAGTCCGGGATATATGAGGCTAAAAGAAAGCCCGGGGAACTCACCACTGTTTTTTTTCCTTGAGTGATCCATAGGGTTTTCGCTGGCTGFFFFFFFFFFFFFFFFFFFFF:FFFFFF,FF,FFFFFFFFFF,FFFFFFFFFFFFF:FF:FFFFFFFFFFFFFF:FFFFF:F:FF:FFFF:FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFNM:i:0MD:Z:127MC:Z:151MAS:i:127XS:i:0Yt:Z:NR A00126:224:H7HMWDSX3:3:1101:25048:1960197Test_seq37464660151M=374891372CCTCACTACTCAGCCTTCTCTGGCACCACCCTGGTGTGTGTGGTGGGTAGCAGGGGTTGAAACTCCTTGTTATAGTCTGCTGAGGATGGATGTCTAGGCTCTCCTGTGAGCCTTTGTAGTCGACAGTAGAGATGGGACCACAGTGTTTTCTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFNM:i:0MD:Z:151MC:Z:127M24SAS:i:151XS:i:0Yt:Z:NR 0 60 CCTCACTACTCAGCCTTCTCTGGCACCACCCTGGTGTGTGTGGTGGGTAGCAGGGGTTGAAACTCCTTGTTATAGTCTGCTGAGGATGGATGTCTAGGCTCTCCTGTGAGCCTTTGTAGTCGACAGTAGAGATGGGACCACAGTGTTTTCT +A00126:224:H7HMWDSX3:3:1101:19289:20901 Test_seq 168345 Test_seq 168766 + - UU A00126:224:H7HMWDSX3:3:1101:19289:20901161Test_seq16834560151M=168616422GATCACGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCTTGCCAATCCGAATACCCTTTATTTCTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFF,FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:6MD:Z:1G3T14A28T1G98T0MC:Z:151MAS:i:129XS:i:71Yt:Z:UU A00126:224:H7HMWDSX3:3:1101:19289:2090181Test_seq16861660151M=168345-422TTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:100T50MC:Z:151MAS:i:146XS:i:71Yt:Z:UU 60 60 GATCACGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCTTGCCAATCCGAATACCCTTTATTTCTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCC TTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATC +A00126:224:H7HMWDSX3:3:1101:9218:20870 Test_seq 169231 Test_seq 169460 + - UU A00126:224:H7HMWDSX3:3:1101:9218:2087097Test_seq16923160151M=169310230ATTCTTTTAATTTCATTTGGCTCTGTTGTGATGTGGTCCTTCTCGTTTCTTATTTGGGTTATTTGTTTCCTTTCCTGTTTTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::,:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF::FFFFFFFFFFFF:FFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFF,F:,,FFFFFFFFF:F:FFFNM:i:2MD:Z:37T16C96MC:Z:151MAS:i:141XS:i:72Yt:Z:UU A00126:224:H7HMWDSX3:3:1101:9218:20870145Test_seq16931060151M=169231-230TTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCF:FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:110Yt:Z:UU 60 60 ATTCTTTTAATTTCATTTGGCTCTGTTGTGATGTGGTCCTTCTCGTTTCTTATTTGGGTTATTTGTTTCCTTTCCTGTTTTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGT TTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCC +A00126:224:H7HMWDSX3:3:1101:12933:23672 ! 0 Test_seq 168704 - - NR A00126:224:H7HMWDSX3:3:1101:12933:23672161Test_seq1683476021S130M=168554358ATACCCATCTGCACAGATTGATCATGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCTTGCCAATCCGGATACCCTTTATTTCTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGTGGTGATAAAGGFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,F:FFF:FFFFFFFFFFFFFFFNM:i:3MD:Z:18A28T70C11MC:Z:151MAS:i:115XS:i:67Yt:Z:NR A00126:224:H7HMWDSX3:3:1101:12933:2367281Test_seq16855460151M=168347-358TTGAATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATCATGTGGTTTTTATCTTTTGTTTTATTTATFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:3C147MC:Z:21S130MAS:i:147XS:i:82Yt:Z:NR 0 60 TTGAATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATCATGTGGTTTTTATCTTTTGTTTTATTTAT +A00126:224:H7HMWDSX3:3:1101:4869:24142 Test_seq 170390 Test_seq 170831 + - UU A00126:224:H7HMWDSX3:3:1101:4869:24142161Test_seq17039060151M=170681442TCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTTTTCATGTGTGTTGTTGGCCATTTCATTTTTCCACATACTTTTTTGTGCTGAGACGTTTTTCTTAGTAGATFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF,FFFFFFFFFFFFF:F,FFFFFFFFFFFFFFFF:F,F::F:FFFFNM:i:1MD:Z:100T50MC:Z:151MAS:i:146XS:i:34Yt:Z:UU A00126:224:H7HMWDSX3:3:1101:4869:2414281Test_seq17068160151M=170390-442CGTTCTATATCGCCTTGTATCACTCTCCATCTGGCAGTTCAATGCCTCCTATATTTAGTCCCTCTTTTTGATTTTTGTGATCGTTTATCTATTGATTTCCATGATTTCCTGTTATGTGTTTTATTTTGTCTATTTATTTATTTTTTGGAATFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFNM:i:7MD:Z:4G6A38G22A45A9T16A4MC:Z:151MAS:i:116XS:i:0Yt:Z:UU 60 60 TCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTTTTCATGTGTGTTGTTGGCCATTTCATTTTTCCACATACTTTTTTGTGCTGAGACGTTTTTCTTAGTAGAT CGTTCTATATCGCCTTGTATCACTCTCCATCTGGCAGTTCAATGCCTCCTATATTTAGTCCCTCTTTTTGATTTTTGTGATCGTTTATCTATTGATTTCCATGATTTCCTGTTATGTGTTTTATTTTGTCTATTTATTTATTTTTTGGAAT +A00126:224:H7HMWDSX3:3:1101:30590:25285 ! 0 Test_seq 168735 - + NR A00126:224:H7HMWDSX3:3:1101:30590:25285145Test_seq16899060115M36S=168735-370TGGTGTTAAGTCTTCTCTGAAGGTTTGGTAGAACTCTGCAGTGAAGCCGTCTGGGCCGGGACTTTTTTTTGTTGGAAGTTTTTTGATTACCGTTTCAATCTCTTTTTTTGTTATGAGTGATCCCTTCCCTATTTTGCTCTGCTAAAACCTCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:57A57MC:Z:151MAS:i:110XS:i:51Yt:Z:NR A00126:224:H7HMWDSX3:3:1101:30590:2528597Test_seq16873560151M=168990370ATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTCTATTGGCTAGAATTTTGTTGAGGATTTTTGCATCTATGTTCATGAGGGATATAGGTCTAAAATTTTCTTTTTTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,:F:FF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F,FFFFFFFFFF,:FFFFFFFF:FF,FFFFFFFFFFFNM:i:0MD:Z:151MC:Z:115M36SAS:i:151XS:i:85Yt:Z:NR 0 60 ATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTCTATTGGCTAGAATTTTGTTGAGGATTTTTGCATCTATGTTCATGAGGGATATAGGTCTAAAATTTTCTTTTTTT +A00126:224:H7HMWDSX3:3:1101:21441:26631 ! 0 Test_seq 264606 - + NR A00126:224:H7HMWDSX3:3:1101:21441:26631145Test_seq26485060116M35S=264606-360TGTGTGGTTATCCTCCATTTTGAGAATTTCCTATGTGTTATAAATCATAATCTCTGCCTGTGGTTAAAAGGATTAGGGTGGGACATCATACTCTTGCTCAGGCCGCATCCCTGATCGATCGATAGAGTTTGGATTTTCCTGTAAGAGGTTAFFFFFFFFF::FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFF,FFFFFFFFFFNM:i:0MD:Z:116MC:Z:151MAS:i:116XS:i:58Yt:Z:NR A00126:224:H7HMWDSX3:3:1101:21441:2663197Test_seq26460660151M=264850360TGCCACTGGTTAGCATCTGAACTCTTAACCACTGTGCCACCAGGGCTTCTTCCATGTGTCACCAGAGCGAGCTTCTTCCAGGACTACCCTAATATAAAGACTGGTTCGTAATAGACGTTCAGTGAAGACTGAGTGGGTGATGGCTGAATGGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFNM:i:0MD:Z:151MC:Z:116M35SAS:i:151XS:i:0Yt:Z:NR 0 60 TGCCACTGGTTAGCATCTGAACTCTTAACCACTGTGCCACCAGGGCTTCTTCCATGTGTCACCAGAGCGAGCTTCTTCCAGGACTACCCTAATATAAAGACTGGTTCGTAATAGACGTTCAGTGAAGACTGAGTGGGTGATGGCTGAATGG +A00126:224:H7HMWDSX3:3:1101:23999:27367 Test_seq 167317 Test_seq 214045 - - UU A00126:224:H7HMWDSX3:3:1101:23999:27367113Test_seq16716752151M=21389546729GCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAATCATGTAGATTTTAGAGAFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:76T74MC:Z:151MAS:i:146XS:i:121XA:Z:Test_seq,+213717,151M,6;Yt:Z:UU A00126:224:H7HMWDSX3:3:1101:23999:27367177Test_seq21389522151M=167167-46729GATGAGATTCCATCTCACACCAGCAAGGCTGGCATTAATCCAAAAAACACAAAATAATAACTGTTGGAGAGGCTGCGGAGAGATTGGAACTCTCATACACTGCTGGTGGGAATGTAAAATGGTACAACCACTTTGGAAATGTATCTGGCGTF:FFFFF:,FF,FFFFFFFFFFFFFFFFF:FFFFFFFFF,FFFFFF:FFFF:,FFF:FFF,:FFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFNM:i:7MD:Z:18T3A37A22C9T46C7T2MC:Z:151MAS:i:118XS:i:106XA:Z:Test_seq,+166989,151M,9;Yt:Z:UU 52 22 GCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAATCATGTAGATTTTAGAGA GATGAGATTCCATCTCACACCAGCAAGGCTGGCATTAATCCAAAAAACACAAAATAATAACTGTTGGAGAGGCTGCGGAGAGATTGGAACTCTCATACACTGCTGGTGGGAATGTAAAATGGTACAACCACTTTGGAAATGTATCTGGCGT +A00126:224:H7HMWDSX3:3:1101:31458:29951 Test_seq 168066 Test_seq 213321 - - UU A00126:224:H7HMWDSX3:3:1101:31458:29951113Test_seq16791660151M=21317145256TATGAAATTAGTGATTTGTTTCTCTATCCCCTTAAAATATGACATTGGTATTTGGATTGGAAGTGCGTTATATATATAGATGGCTTTTGGTAGAATAGACATTTTTACTATGTTAAGTCTTCCTATCCATGAGCAGGGTATGTTCTTCCACF:FF::FF,FFFFFF:FF::FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:::FFFFFFF:FFF:FF,FF:FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFNM:i:1MD:Z:73G77MC:Z:151MAS:i:146XS:i:58Yt:Z:UU A00126:224:H7HMWDSX3:3:1101:31458:29951177Test_seq2131717151M=167916-45256AAGAAAGTGGGAGGCCTCACTCTACCTGATTTCAGAACCTATTATACAGCTACAGTAGTCAAAACAGCCTGGTACTGGTACAAGAACAGGCACATAGACCAATGGAACAGAATTGAGAACCCAGATATAAATCCATCCATGTATGAGCAGC,FF::,FFFFFFFFFFFFFFFFF,F:FFFF:FFFFFFFFFFFF,F,FFFF,FFFFFFFFF,::FFFF,FFFFFFFFFFFFFF:FFFFFFFFFFFF,F:FFFFFFFFFF:F,FFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFNM:i:2MD:Z:83C57A9MC:Z:151MAS:i:141XS:i:136XA:Z:Test_seq,+167717,151M,3;Yt:Z:UU 60 7 TATGAAATTAGTGATTTGTTTCTCTATCCCCTTAAAATATGACATTGGTATTTGGATTGGAAGTGCGTTATATATATAGATGGCTTTTGGTAGAATAGACATTTTTACTATGTTAAGTCTTCCTATCCATGAGCAGGGTATGTTCTTCCAC AAGAAAGTGGGAGGCCTCACTCTACCTGATTTCAGAACCTATTATACAGCTACAGTAGTCAAAACAGCCTGGTACTGGTACAAGAACAGGCACATAGACCAATGGAACAGAATTGAGAACCCAGATATAAATCCATCCATGTATGAGCAGC +A00126:224:H7HMWDSX3:3:1101:22001:30201 Test_seq 366564 Test_seq 369651 + - UR A00126:224:H7HMWDSX3:3:1101:22001:3020197Test_seq3665641822M129S=3695013088AGGATTTCATTTTATTTGGATCTAAAAAGAAAAGGGTGGTAGTGGGGGGAAACCTAAAACAAATCCTACTGCTGTTAAGATTATTATTAGGTTACCTTACCATAAGTGACTGTAGACTATTGTGGAAGGAATCCTGGTGTCACAGTGGTTAFF,F:FFFFF:,:FF:FFFF,,:FF:FFF:FF:F,:FF,,F::,,:,:,FFF,,FFFFF,FFFF,FFFF,,,F,:,FFFFFF:,,FFFFF,FFF:FF:,:,FFFFF,,FF,,FF:FF,FFF,F,,FFFFFFF::,:,::,:FFF:F:,:,FNM:i:0MD:Z:22MC:Z:151MAS:i:22XS:i:0SA:Z:Test_seq,369346,+,22S129M,60,2;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1101:22001:302012145Test_seq3693466022H129M=369501306TAAAAAGAAAAGGGTGGTAGTGGGGGGAAACCTAAAACAAATCCTACTGCTGTTAAGATTATTATTAGGTTACCTTACCATAAGTGACTGTAGACTATTGTGGAAGGAATCCTGGTGTCACAGTGGTTA:FF:FFF:FF:F,:FF,,F::,,:,:,FFF,,FFFFF,FFFF,FFFF,,,F,:,FFFFFF:,,FFFFF,FFF:FF:,:,FFFFF,,FF,,FF:FF,FFF,F,,FFFFFFF::,:,::,:FFF:F:,:,FNM:i:2MD:Z:76T40G11MC:Z:151MAS:i:119XS:i:0SA:Z:Test_seq,366564,+,22M129S,18,0;Yt:Z:UR A00126:224:H7HMWDSX3:3:1101:22001:30201145Test_seq36950160151M=366564-3088AGCAGTTCGAACCCACCAGTGGCTTTGTGGGAGAAAAGACCTGACAATTTGCTTCTGTAAAGATTGTTACTCTTGTTAGGTGCCGTCAAGTCTGTTCCAACTCACAGCCACCCTATGTACAATAGAAGGAAACACAGCCCAGTCCTGTGCCFFF:FFFF,FFF,F,,,,FFF:FFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFF,FF:FFFFFFFFFFFFFFF,FFFF,FFF,FFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFF::FFFFF:FF:FF:FFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:22M129SAS:i:151XS:i:19Yt:Z:UR 18 60 AGGATTTCATTTTATTTGGATCTAAAAAGAAAAGGGTGGTAGTGGGGGGAAACCTAAAACAAATCCTACTGCTGTTAAGATTATTATTAGGTTACCTTACCATAAGTGACTGTAGACTATTGTGGAAGGAATCCTGGTGTCACAGTGGTTA AGCAGTTCGAACCCACCAGTGGCTTTGTGGGAGAAAAGACCTGACAATTTGCTTCTGTAAAGATTGTTACTCTTGTTAGGTGCCGTCAAGTCTGTTCCAACTCACAGCCACCCTATGTACAATAGAAGGAAACACAGCCCAGTCCTGTGCC +A00126:224:H7HMWDSX3:3:1101:28736:30655 Test_seq 169255 Test_seq 169484 + - UU A00126:224:H7HMWDSX3:3:1101:28736:3065597Test_seq16925560151M=169334230GTTGTGATGTGGTCCTTCTCGTTTCTTATTCGGGTTATTTGTTTCCTTTCCTGTGTTTCTTTAGTCGGTCTGGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF,FFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFF,FFFNM:i:4MD:Z:13T40T11A4A79MC:Z:151MAS:i:131XS:i:88Yt:Z:UU A00126:224:H7HMWDSX3:3:1101:28736:30655145Test_seq16933460151M=169255-230GTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTGGTTTTCTTCTGGTGCCTGATGGGTTCTTTTGTTGCTCACTFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:110T40MC:Z:151MAS:i:146XS:i:103Yt:Z:UU 60 60 GTTGTGATGTGGTCCTTCTCGTTTCTTATTCGGGTTATTTGTTTCCTTTCCTGTGTTTCTTTAGTCGGTCTGGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGT GTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTGGTTTTCTTCTGGTGCCTGATGGGTTCTTTTGTTGCTCACT +A00126:224:H7HMWDSX3:3:1101:13774:31015 Test_seq 170280 Test_seq 170720 + - UU A00126:224:H7HMWDSX3:3:1101:13774:3101597Test_seq17028060151M=170582441TTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCTGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTFFFFFFFFFF,FFFFF:FFFFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFF:F:FFFFFFFFF:FFFF:FFFFFFFFFFFFF,FFFFF,NM:i:1MD:Z:96C54MC:Z:16M12I123MAS:i:146XS:i:83Yt:Z:UU A00126:224:H7HMWDSX3:3:1101:13774:31015145Test_seq1705826016M12I123M=170280-441TGAGTCGTTACGTTTTTCTTGGTTTTTGTCTTGAGTTATAGAGTTGTTATACCTTTTTGTGGTTACCTCATTATATACCCCTATTTTTCTAAGTAAAAACCTAACTTGTATTGTTCTATATCGCCTTGTATCACTCTCCATATGGCAGTTCFFFFF:FFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF,FFNM:i:22MD:Z:27G2A3A3T17T5T36C3G6A18C9MC:Z:151MAS:i:73XS:i:19Yt:Z:UU 60 60 TTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCTGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATT TGAGTCGTTACGTTTTTCTTGGTTTTTGTCTTGAGTTATAGAGTTGTTATACCTTTTTGTGGTTACCTCATTATATACCCCTATTTTTCTAAGTAAAAACCTAACTTGTATTGTTCTATATCGCCTTGTATCACTCTCCATATGGCAGTTC +A00126:224:H7HMWDSX3:3:1101:31629:31062 Test_seq 365751 Test_seq 372668 + + RU A00126:224:H7HMWDSX3:3:1101:31629:3106265Test_seq36575160151M=3726686918CACACTCATAAAACAAGAAAAACACATGAGTTGTTTTTAAAGCCAATGAAATGCAGAGATTATCAAACAATATCGTTAATATGACACGCAAGTAAAATTATACTGAAGATCACTCAAAAACGGTTGCAGCAACTGCCAGAAATTCAGGCCTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFF:F:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,F,FF,FFFFFNM:i:0MD:Z:151MC:Z:24M127SAS:i:151XS:i:0Yt:Z:RU A00126:224:H7HMWDSX3:3:1101:31629:31062129Test_seq3726683024M127S=365751-6918TCATCCAGGTTGTTGCATGTTTTAGATCCATCTGACATCAGCAATGATATCCCTGGTTCCACATCCTCTTCTGAAACAGGCCTGAATTTCTGGCAGTTGCTGCAACCGTTTTTGAGTGATCTTCAGTATAATTTTACTTGCGTGTCATATTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFF,FFNM:i:0MD:Z:24MC:Z:151MAS:i:24XS:i:0SA:Z:Test_seq,365828,-,127M24S,60,0;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1101:31629:310622193Test_seq36582860127M24H=365751-204AATATGACACGCAAGTAAAATTATACTGAAGATCACTCAAAAACGGTTGCAGCAACTGCCAGAAATTCAGGCCTGTTTCAGAAGAGGATGTGGAACCAGGGATATCATTGCTGATGTCAGATGGATCFF,FFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:127MC:Z:151MAS:i:127XS:i:54SA:Z:Test_seq,372668,+,24M127S,30,0;Yt:Z:RU 60 30 CACACTCATAAAACAAGAAAAACACATGAGTTGTTTTTAAAGCCAATGAAATGCAGAGATTATCAAACAATATCGTTAATATGACACGCAAGTAAAATTATACTGAAGATCACTCAAAAACGGTTGCAGCAACTGCCAGAAATTCAGGCCT TCATCCAGGTTGTTGCATGTTTTAGATCCATCTGACATCAGCAATGATATCCCTGGTTCCACATCCTCTTCTGAAACAGGCCTGAATTTCTGGCAGTTGCTGCAACCGTTTTTGAGTGATCTTCAGTATAATTTTACTTGCGTGTCATATT +A00126:224:H7HMWDSX3:3:1101:32398:33238 ! 0 ! 0 - - MM A00126:224:H7HMWDSX3:3:1101:32398:3323881Test_seq449330059M=383985-65404TCCTGGCTGAAAGCAGAGAATACCAGAAAGATGTTTACCTGTGTTTTATTGACTATGCAF,F:FFFFFFFFFF:FFFF,:FFFFFF,FFFFFF:FFFFFFFFFFFFF::FFFF:FFFFNM:i:1MD:Z:18C40MC:Z:59MAS:i:54XS:i:54Yt:Z:MM A00126:224:H7HMWDSX3:3:1101:32398:33238161Test_seq383985059M=44933065404TTCTGGCTGAAAGCAGAGAATACCAGAAAGATGTGTACCTGTGTTGTCATGACTATGCAFFFFFFFFFFFFFFFFFFF:FFFFF,F::FFFF::FFFFFFFFF:,F,,FFFFFFFFF,NM:i:6MD:Z:1C26G5T10T1A0T10MC:Z:59MAS:i:33XS:i:33XA:Z:Test_seq,-333519,59M,6;Test_seq,-116683,56M3S,5;Test_seq,-316244,10M2I47M,7;Yt:Z:MM 0 0 TCCTGGCTGAAAGCAGAGAATACCAGAAAGATGTTTACCTGTGTTTTATTGACTATGCA TTCTGGCTGAAAGCAGAGAATACCAGAAAGATGTGTACCTGTGTTGTCATGACTATGCA +A00126:224:H7HMWDSX3:3:1101:18602:33395 Test_seq 171431 Test_seq 171865 + - UU A00126:224:H7HMWDSX3:3:1101:18602:3339597Test_seq17143160151M=171715435CACAAGTTATCCTTCTTGATAGAGTCCCACATGATTCTTAGGGTTTCTTCATTTTTTTAAATTCTTTTATCTGATTTTTTTTCAGCTATGTTGGTGTTGATTCCCTGGTCCTCCAGATGTCCCAGTCTACATTCTAATTGCTCGAGTCTGCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:FFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFFFF:FF::FFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:4MD:Z:1G56T35A22A33MC:Z:151MAS:i:134XS:i:47Yt:Z:UU A00126:224:H7HMWDSX3:3:1101:18602:33395145Test_seq17171560151M=171431-435TTCTTCAACAGTTTTATCAGTGTGTTCCTTGGCTTTTTCTGCAGTTATCCTAATTTCATTTGTGATATCTTTAAGCATTCTGTAAATTAGTTTTTTATATTCTGTATCTGATAATTCCAGGATTGTATCTTCATTTGGGAAAGATTTTGATF::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,,:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:2T148MC:Z:151MAS:i:148XS:i:0Yt:Z:UU 60 60 CACAAGTTATCCTTCTTGATAGAGTCCCACATGATTCTTAGGGTTTCTTCATTTTTTTAAATTCTTTTATCTGATTTTTTTTCAGCTATGTTGGTGTTGATTCCCTGGTCCTCCAGATGTCCCAGTCTACATTCTAATTGCTCGAGTCTGC TTCTTCAACAGTTTTATCAGTGTGTTCCTTGGCTTTTTCTGCAGTTATCCTAATTTCATTTGTGATATCTTTAAGCATTCTGTAAATTAGTTTTTTATATTCTGTATCTGATAATTCCAGGATTGTATCTTCATTTGGGAAAGATTTTGAT +A00126:224:H7HMWDSX3:3:1101:13042:33630 Test_seq 166363 Test_seq 166687 + - UU A00126:224:H7HMWDSX3:3:1101:13042:3363097Test_seq16636360151M=166544325TTTGCCCGTTGACTTAGAAGTCTCCTCAAACCATGTTCCCCAGACCCCAGCCCCTGCTCCGCTGACCTTTGAAGCTTTCATTTTATCCCGGAAACCTCTTTGCTTTTAGTCCAGTCCAATTAGGCTGACCTTCCTTGTATTGTGTGTTGTCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFF,F:FFF:FF:,FFF,FFFF:F,:FFFFFFFF::FF:F,NM:i:0MD:Z:151MC:Z:5M3I18M4I121MAS:i:151XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1101:13042:33630145Test_seq166544605M3I18M4I121M=166363-325CTGATTGATGATCAATAAAAAACCCTCTCCCTCCCTCCCTCCCTCCCTCCCCCCTTTGTAACCACATAAGTATGTGTTCTTCTCCGTTTTTTCTATTTCTCAAGATCTTATAATAGTGGTCTTATACAATATTTGTCCTTTTGCCTCTGACF:,FFFFFFFFFFFFF:FFFFFF:F,FFFFFFFF:FF,FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF:F:FFFFFFFF:F:FFFFFF:,FFFFF,FFFFFNM:i:9MD:Z:59A34C49MC:Z:151MAS:i:119XS:i:62Yt:Z:UU 60 60 TTTGCCCGTTGACTTAGAAGTCTCCTCAAACCATGTTCCCCAGACCCCAGCCCCTGCTCCGCTGACCTTTGAAGCTTTCATTTTATCCCGGAAACCTCTTTGCTTTTAGTCCAGTCCAATTAGGCTGACCTTCCTTGTATTGTGTGTTGTC CTGATTGATGATCAATAAAAAACCCTCTCCCTCCCTCCCTCCCTCCCTCCCCCCTTTGTAACCACATAAGTATGTGTTCTTCTCCGTTTTTTCTATTTCTCAAGATCTTATAATAGTGGTCTTATACAATATTTGTCCTTTTGCCTCTGAC +A00126:224:H7HMWDSX3:3:1101:29966:36072 Test_seq 170104 Test_seq 170357 + - UU A00126:224:H7HMWDSX3:3:1101:29966:36072161Test_seq17010460151M=170207254TCATTGGGTGCATAAATATTTAATATGGTTATGTCTTCCTGATCAATTGTCCCTTTTATCATTATGTAGTGTCCTTCTTTATCCTTTGTGGTGGATTTAAGTCTAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCCTCTTCTTTTTTFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF::FFFFF:F:FFFFFFFFFFFFFF:,:FFFF:F:FFFFFFFFFFNM:i:6MD:Z:3C7G20A32A25C0A58MC:Z:151MAS:i:122XS:i:49Yt:Z:UU A00126:224:H7HMWDSX3:3:1101:29966:3607281Test_seq17020760151M=170104-254TAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCCTCTTCTTTTTTGCTTATTGTTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCFFFFFFF,FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFF:F:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFNM:i:1MD:Z:55A95MC:Z:151MAS:i:146XS:i:88Yt:Z:UU 60 60 TCATTGGGTGCATAAATATTTAATATGGTTATGTCTTCCTGATCAATTGTCCCTTTTATCATTATGTAGTGTCCTTCTTTATCCTTTGTGGTGGATTTAAGTCTAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCCTCTTCTTTTTT TAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCCTCTTCTTTTTTGCTTATTGTTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATC +A00126:224:H7HMWDSX3:3:1101:3595:36620 Test_seq 167567 Test_seq 167976 + - UU A00126:224:H7HMWDSX3:3:1101:3595:36620161Test_seq1675673015S136M=167826410ACCAACCAGCTAGACTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATTGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGATAAAAAGACTATCATTTCCCCAAATGACTGACACTGGGCCFFFFFFFF,,FF:FFFFFF,FF:FFFFF:FFFFF:FFFFF:FFFFFFFFFF:FFFF,FF:,,FFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFF,FFFFFFFF:,:F,FFFF:FFFFFFF:F:FFFFF,FF,F:FF,:FFFFFFFF::NM:i:4MD:Z:41G53T23T13T2MC:Z:151MAS:i:118XS:i:103XA:Z:Test_seq,-213336,124M2I10M15S,7;Yt:Z:UU A00126:224:H7HMWDSX3:3:1101:3595:3662081Test_seq16782660151M=167567-410ATAGGTTCTGAAATCAGGTAGAGTGAGGCCTCCCACTTTCTTCTTCTTTTTCAGTAATGCTTTGCTTATCCGGGGGTTCTTTCCCTTCCACATGAAATTAGTGATTTGTTTCTCTATCCCCTTAAAATATGACATTGGTATTTGGATTGGAFFFFFFFFFFFFF::FFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFF::F,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFF,FFFFFFF:FF,FF:,FFFFFFFFFFFFFFFNM:i:1MD:Z:90T60MC:Z:15S136MAS:i:146XS:i:87Yt:Z:UU 30 60 ACCAACCAGCTAGACTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATTGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGATAAAAAGACTATCATTTCCCCAAATGACTGACACTGGGCC ATAGGTTCTGAAATCAGGTAGAGTGAGGCCTCCCACTTTCTTCTTCTTTTTCAGTAATGCTTTGCTTATCCGGGGGTTCTTTCCCTTCCACATGAAATTAGTGATTTGTTTCTCTATCCCCTTAAAATATGACATTGGTATTTGGATTGGA +A00126:224:H7HMWDSX3:3:1102:11306:1908 Test_seq 186004 Test_seq 256018 - - RU A00126:224:H7HMWDSX3:3:1102:11306:1908177Test_seq18585460151M=25599370015ATGTGCCAGGTGCTGTTTTATGCTTTCATCTTCACTACTTCAGTCAGTCCTCACTGCAAACCAGTGAGGCAGATAGTATTATTCCCACTTTACAGTTGAAGAGACTGAGGCTCTATGAAATTAAGAAAATGTCCCCAGCCACAGGGCTAGTFFFFF:::F,F:FF:F:FFFF::FFF,FF,F,F:FFF:F:,F:FFFFFF:FFFFFF:FFF,:F::FFFF:FFFF:FFFFFFFFFFFFFFFFF:F:FF,FFFFFFF:FFFFFF,F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:31G119MC:Z:125S26MAS:i:146XS:i:0Yt:Z:RU A00126:224:H7HMWDSX3:3:1102:11306:19082145Test_seq1856846026H125M=185854321GATCAAAATTATTACTAATATAAAAAAATCAATAAGCTGGAAAATAAAGCAGAAGACACTTTTGAAAAATTAGAGCAAAATGACATTGTTGGAAAATATGAGGGAAAGAATAGCTAAATACTACTFFFFFFFFF,FFFFFFFFFFFF:FFFFF,FFFFF:FFF,,FFFFFFFFFFFFFF,F,FFF::FFFFFF,F:F:FF,F:FFFFFFFF,F,F,FFFFF,F:,FF,FF:F,:FFF:,:F:F::,FF,,NM:i:3MD:Z:68T17G1G36MC:Z:151MAS:i:110XS:i:0SA:Z:Test_seq,255993,-,125S26M,42,0;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1102:11306:1908113Test_seq25599342125S26M=185854-70015AGTAGTATTTAGCTATTCTTTCCCTCATATTTTCCAACAATGTCATTTTGCTCTAATTTTTCAAAAGTGTCTTCTGCTTTATTTTCCAGCTTATTGATTTTTTTATATTAGTAATAATTTTGATCGATCTAAATGCCCAATCAACCAACTT,,FF,::F:F:,:FFF:,F:FF,FF,:F,FFFFF,F,F,FFFFFFFF:F,FF:F:F,FFFFFF::FFF,F,FFFFFFFFFFFFFF,,FFF:FFFFF,FFFFF:FFFFFFFFFFFF,FFFFFFFFF:FFFFF,F,F:FFFFFFFFFFFFFFFNM:i:0MD:Z:26MC:Z:151MAS:i:26XS:i:0SA:Z:Test_seq,185684,+,26S125M,60,3;Yt:Z:RU 60 42 ATGTGCCAGGTGCTGTTTTATGCTTTCATCTTCACTACTTCAGTCAGTCCTCACTGCAAACCAGTGAGGCAGATAGTATTATTCCCACTTTACAGTTGAAGAGACTGAGGCTCTATGAAATTAAGAAAATGTCCCCAGCCACAGGGCTAGT AGTAGTATTTAGCTATTCTTTCCCTCATATTTTCCAACAATGTCATTTTGCTCTAATTTTTCAAAAGTGTCTTCTGCTTTATTTTCCAGCTTATTGATTTTTTTATATTAGTAATAATTTTGATCGATCTAAATGCCCAATCAACCAACTT +A00126:224:H7HMWDSX3:3:1102:7491:2785 ! 0 Test_seq 168666 - - NR A00126:224:H7HMWDSX3:3:1102:7491:278597Test_seq1682384324S127M=168516429CACTTGATGACCCAAAATCAGATTAATCCAAGTGATTTTTGTATGTTTATTTTATAACCTGAGACTCTTCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGGTTTTCCATGTATACGATCATGTCATCTGCAAATAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFNM:i:2MD:Z:33T74G18MC:Z:151MAS:i:117XS:i:97XA:Z:Test_seq,-212674,127M24S,6;Yt:Z:NR A00126:224:H7HMWDSX3:3:1102:7491:2785145Test_seq16851660151M=168238-429CAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAGTTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTFFF::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF,FFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:79A71MC:Z:24S127MAS:i:146XS:i:82Yt:Z:NR 0 60 CAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAGTTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATT +A00126:224:H7HMWDSX3:3:1102:22987:3724 Test_seq 272454 Test_seq 288164 - + UR A00126:224:H7HMWDSX3:3:1102:22987:37242193Test_seq28841260128M23H=288164-376CACTATTTTCTCAACCTCAATGTCATTTGTCCTTAAATGACACCCACTTCCATACCACCGCTCATTCAGGTTTTCTTTTATCTGTGTATCCCTAAAGACAAACAAATCTCAATGACGTCTGTGACTTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:128MC:Z:151MAS:i:128XS:i:0SA:Z:Test_seq,272431,-,127S24M,30,0;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1102:22987:3724145Test_seq27243130127S24M=28816415711CACTATTTTCTCAACCTCAATGTCATTTGTCCTTAAATGACACCCACTTCCATACCACCGCTCATTCAGGTTTTCTTTTATCTGTGTATCCCTAAAGACAAACAAATCTCAATGACGTCTGTGACTTTTAAAATGTACAGCCATCATGAAGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:24MC:Z:151MAS:i:24XS:i:0SA:Z:Test_seq,288412,-,128M23S,60,0;Yt:Z:UR A00126:224:H7HMWDSX3:3:1102:22987:372497Test_seq28816460151M=272431-15711TAAGGCATGGTAGAACCAGAGATGTCCCACTACACTGCTTTCCACCTGGGGACACTGGGTACGGGAAAGGTTCCCTTAGAGTTCTAGCTTTTCTTTTTTTGAAGAATTTATACAACAGGGAATTTTGAGAAGCCAGCTAAAATGCCCACGAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF,FFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:127S24MAS:i:151XS:i:0Yt:Z:UR 30 60 CACTATTTTCTCAACCTCAATGTCATTTGTCCTTAAATGACACCCACTTCCATACCACCGCTCATTCAGGTTTTCTTTTATCTGTGTATCCCTAAAGACAAACAAATCTCAATGACGTCTGTGACTTTTAAAATGTACAGCCATCATGAAG TAAGGCATGGTAGAACCAGAGATGTCCCACTACACTGCTTTCCACCTGGGGACACTGGGTACGGGAAAGGTTCCCTTAGAGTTCTAGCTTTTCTTTTTTTGAAGAATTTATACAACAGGGAATTTTGAGAAGCCAGCTAAAATGCCCACGA +A00126:224:H7HMWDSX3:3:1102:11822:4335 Test_seq 166209 Test_seq 214876 - - UU A00126:224:H7HMWDSX3:3:1102:11822:4335113Test_seq1661592651M=21482648668CAGGATAATGTAGGTCTCTGGTTCATGTCGCCCTTTCTGTCTCTTGGGTTCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:28G22MC:Z:51MAS:i:46XS:i:38XA:Z:Test_seq,+214826,51M,3;Yt:Z:UU A00126:224:H7HMWDSX3:3:1102:11822:4335177Test_seq2148262851M=166159-48668GAGCCCAAGAGACAGAAAGGGCCACATGAACCAGAGACCTACATCATCCTGFF:FFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFNM:i:0MD:Z:51MC:Z:51MAS:i:51XS:i:43XA:Z:Test_seq,+166159,51M,2;Yt:Z:UU 26 28 CAGGATAATGTAGGTCTCTGGTTCATGTCGCCCTTTCTGTCTCTTGGGTTC GAGCCCAAGAGACAGAAAGGGCCACATGAACCAGAGACCTACATCATCCTG +A00126:224:H7HMWDSX3:3:1102:31313:5556 Test_seq 151016 Test_seq 222264 + - RU A00126:224:H7HMWDSX3:3:1102:31313:555697Test_seq15101660151M=22223571249TCCCTCACTGTTCTTTATCGATGTGTAGTATTCCATTCTGTGAATATGCCATAATTTATTTATCCATTCATCTGTTGATGGGCATCTTGGTTGCTTCCAGCTTCTTGCTGTTATAAACAGTGCTGCAATAAACATGGGTGTGCATATATCTFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFNM:i:0MD:Z:151MC:Z:121S30MAS:i:151XS:i:22Yt:Z:RU A00126:224:H7HMWDSX3:3:1102:31313:55562193Test_seq15130047121M30H=151016-405CCACAAGCAGTGTATAAGTGTTCCAATCTCTCCACAGCCTCTCCAACATTTATTATTTTGTGTTTTTTGGATTAATGCCAGCCTTGTTGGAGTGAGATGAAATCTCATTGTAGTTTTGATCFFFF:FFFFFFFFFFFFFFF,FFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFNM:i:0MD:Z:121MC:Z:151MAS:i:121XS:i:101SA:Z:Test_seq,222235,-,121S30M,60,0;XA:Z:Test_seq,-48995,15M2I104M30S,4;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1102:31313:5556145Test_seq22223560121S30M=151016-71249CCACAAGCAGTGTATAAGTGTTCCAATCTCTCCACAGCCTCTCCAACATTTATTATTTTGTGTTTTTTGGATTAATGCCAGCCTTGTTGGAGTGAGATGAAATCTCATTGTAGTTTTGATCGATCCATTTTGAATTAGTTTTTGTGTATGCFFFF:FFFFFFFFFFFFFFF,FFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:30MC:Z:151MAS:i:30XS:i:0SA:Z:Test_seq,151300,-,121M30S,47,0;Yt:Z:RU 60 60 TCCCTCACTGTTCTTTATCGATGTGTAGTATTCCATTCTGTGAATATGCCATAATTTATTTATCCATTCATCTGTTGATGGGCATCTTGGTTGCTTCCAGCTTCTTGCTGTTATAAACAGTGCTGCAATAAACATGGGTGTGCATATATCT CCACAAGCAGTGTATAAGTGTTCCAATCTCTCCACAGCCTCTCCAACATTTATTATTTTGTGTTTTTTGGATTAATGCCAGCCTTGTTGGAGTGAGATGAAATCTCATTGTAGTTTTGATCGATCCATTTTGAATTAGTTTTTGTGTATGC +A00126:224:H7HMWDSX3:3:1102:31394:5541 Test_seq 151016 Test_seq 222264 + - RU A00126:224:H7HMWDSX3:3:1102:31394:554197Test_seq15101660151M=22223571249TCCCTCACTGTTCTTTATCGATGTGTAGTATTCCATTCTGTGAATATGCCATAATTTATTTATCCATTCATCTGTTGATGGGCATCTTGGTTGCTTCCAGCTTCTTGCTGTTATAAACAGTGCTGCAATAAACATGGGTGTGCATATATCTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:FFFF:FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFF:FFFFFF:F:FFFNM:i:0MD:Z:151MC:Z:121S30MAS:i:151XS:i:22Yt:Z:RU A00126:224:H7HMWDSX3:3:1102:31394:55412193Test_seq15130047121M30H=151016-405CCACAAGCAGTGTATAAGTGTTCCAATCTCTCCACAGCCTCTCCAACATTTATTATTTTGTGTTTTTTGGATTAATGCCAGCCTTGTTGGAGTGAGATGAAATCTCATTGTAGTTTTGATCFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF,FFFF,FFFFFFFFFFFFFFFFF,FFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFNM:i:0MD:Z:121MC:Z:151MAS:i:121XS:i:101SA:Z:Test_seq,222235,-,121S30M,60,0;XA:Z:Test_seq,-48995,15M2I104M30S,4;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1102:31394:5541145Test_seq22223560121S30M=151016-71249CCACAAGCAGTGTATAAGTGTTCCAATCTCTCCACAGCCTCTCCAACATTTATTATTTTGTGTTTTTTGGATTAATGCCAGCCTTGTTGGAGTGAGATGAAATCTCATTGTAGTTTTGATCGATCCATTTTGAATTAGTTTTTGTGTATGCFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF,FFFF,FFFFFFFFFFFFFFFFF,FFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFF:FFFFF,,FFFFFFFFNM:i:0MD:Z:30MC:Z:151MAS:i:30XS:i:0SA:Z:Test_seq,151300,-,121M30S,47,0;Yt:Z:RU 60 60 TCCCTCACTGTTCTTTATCGATGTGTAGTATTCCATTCTGTGAATATGCCATAATTTATTTATCCATTCATCTGTTGATGGGCATCTTGGTTGCTTCCAGCTTCTTGCTGTTATAAACAGTGCTGCAATAAACATGGGTGTGCATATATCT CCACAAGCAGTGTATAAGTGTTCCAATCTCTCCACAGCCTCTCCAACATTTATTATTTTGTGTTTTTTGGATTAATGCCAGCCTTGTTGGAGTGAGATGAAATCTCATTGTAGTTTTGATCGATCCATTTTGAATTAGTTTTTGTGTATGC +A00126:224:H7HMWDSX3:3:1102:4218:5822 Test_seq 170181 Test_seq 170586 + - UU A00126:224:H7HMWDSX3:3:1102:4218:5822161Test_seq17018160151M=170436406TTTATCCTTTGTGGCGGATTTAAGTCTAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCCTCTTCTTTTTTGCTTATTATTTGCTTGATATGTTTTTTTACTTCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCFFFFFF,FFFFFFFFFFFFFFFFFF:FFFFFFF:FF:F:FFFFFFFFFFFFFFFFFF:FF:FFFFF:FFFFF,F,:FFFFFFFF::FFFFFFF,:FFFF:FF,FFF,FFFFFFF::,F::F:F:FFF:FFFFFFFFF,F:,FFFFF:FF:,NM:i:4MD:Z:15A78A7C1A46MC:Z:151MAS:i:131XS:i:55Yt:Z:UU A00126:224:H7HMWDSX3:3:1102:4218:582281Test_seq17043660151M=170181-406TAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTTTTCATGTGTGTTGTTGGCCTTTTCATTTTTCCACATACTTTTTTGTGCTGAGACGTTTTTCTTAGTAGATTGTGAGATCCTCATTTTCATAATGTTTAACTTTATGTTTGTTGAGTFFFFFFFFFFFFF::F:FFFF,FFFFFFFFF:FFFFF:FFFFFF,FFFFFFFF:FF:FFFF,FFF:FFFFF:FFFFFFFFFF:FFFFF,F,FFFF,F:FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FF,FFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:25Yt:Z:UU 60 60 TTTATCCTTTGTGGCGGATTTAAGTCTAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCCTCTTCTTTTTTGCTTATTATTTGCTTGATATGTTTTTTTACTTCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTC TAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTTTTCATGTGTGTTGTTGGCCTTTTCATTTTTCCACATACTTTTTTGTGCTGAGACGTTTTTCTTAGTAGATTGTGAGATCCTCATTTTCATAATGTTTAACTTTATGTTTGTTGAGT +A00126:224:H7HMWDSX3:3:1102:18602:6652 Test_seq 171219 Test_seq 171678 + - UU A00126:224:H7HMWDSX3:3:1102:18602:6652161Test_seq17121960151M=171528460AAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATGGGGTTCGATGAGCATCTTGGATAGATATCCTTTCGTCTTTCATGATGTCAGGGAAGTTTTCTGTCAGGAGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF,FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:NM:i:1MD:Z:141G9MC:Z:151MAS:i:146XS:i:79Yt:Z:UU A00126:224:H7HMWDSX3:3:1102:18602:665281Test_seq17152860151M=171219-460TGTTTCCCTGGTCCTCCAGAAGTCCCAGCCTACATTCTAATTGCTCGAGTCTGCTCCTCTGACTTTCTATTGTGTTGTCAAATTCTGTAATTTTATTGTTAATCTTTTGGATTTCTACATGCTGTCTCTCTATGGATTCCTGCAACTTGTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:7MD:Z:2A25T26T16C6T59T8A2MC:Z:151MAS:i:120XS:i:53Yt:Z:UU 60 60 AAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATGGGGTTCGATGAGCATCTTGGATAGATATCCTTTCGTCTTTCATGATGTCAGGGAAGTTTTCTGTCAGGAG TGTTTCCCTGGTCCTCCAGAAGTCCCAGCCTACATTCTAATTGCTCGAGTCTGCTCCTCTGACTTTCTATTGTGTTGTCAAATTCTGTAATTTTATTGTTAATCTTTTGGATTTCTACATGCTGTCTCTCTATGGATTCCTGCAACTTGTT +A00126:224:H7HMWDSX3:3:1102:15619:9721 Test_seq 170074 Test_seq 170377 + - UU A00126:224:H7HMWDSX3:3:1102:15619:9721161Test_seq17007425151M=170227304AAAATTTGTTTTATGTATCTTGCAGCCCTGTCATTGGGTGCATAAATATTTAATATGGTTATGTCTTCCTGATCAATTGTCCCTTTTATCATTATGTAGTGTCCTTCTTTATCCTTTGTGGTGGATTTAAGTCTAAAGTCTATTTTGTCAG,,:F::FFFFFFFFFF:FFFFFF,FFFFFFFF::FFFFFF::::::FFF:FF:F:FFFFFFFFFFFF:FFF:FF::FFFFFFFF:FFF:FFF,,FFFFFFF:FFF:FFFFFFFFF:FF:FFFFFFFFF:FFFF:FFFFFFFF:F,FFFFFFNM:i:7MD:Z:8A24C7G20A32A25C0A28MC:Z:151MAS:i:116XS:i:102XA:Z:Test_seq,-38508,147M4S,9;Yt:Z:UU A00126:224:H7HMWDSX3:3:1102:15619:972181Test_seq17022760151M=170074-304ATTAATATTGCTACTCCTCTTCTTTTTTGCTTATTGTTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCGFF:FFFF::FF:FF,FFFF:FFF:F,F:FFFFF,FFF,FFF:F:FFFFFFFF:F,,FFF:FFF:FFFF:F:::FF,FFF::F,FFFFFF::F,FFFFFFFFFFFFFFF:F:FFFFFFFFFF:FFFFFFFFFFFF,FFFF:,:FFF:F,FFFNM:i:1MD:Z:35A115MC:Z:151MAS:i:146XS:i:78Yt:Z:UU 25 60 AAAATTTGTTTTATGTATCTTGCAGCCCTGTCATTGGGTGCATAAATATTTAATATGGTTATGTCTTCCTGATCAATTGTCCCTTTTATCATTATGTAGTGTCCTTCTTTATCCTTTGTGGTGGATTTAAGTCTAAAGTCTATTTTGTCAG ATTAATATTGCTACTCCTCTTCTTTTTTGCTTATTGTTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCG +A00126:224:H7HMWDSX3:3:1102:20645:10473 ! 0 Test_seq 170889 - + NR A00126:224:H7HMWDSX3:3:1102:20645:10473145Test_seq17116660124M27S=170889-401TCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCACTCAAGAAGTACATTTTTCAATGATAAFFFF:FFFFF,FF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF,FFFFFFFFF:FF:FFFFFFFFFFFF,F:F:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:124MC:Z:151MAS:i:124XS:i:52Yt:Z:NR A00126:224:H7HMWDSX3:3:1102:20645:1047397Test_seq17088960151M=171166401CGTTCTGTTTTGTGACCTTGTATTGTGCTGGTACCTGATATTATTGGTCATCAGGCCAAACAATCTCCTTTAGCATTTCTTGCAGTCTTGGTTTAGTTTTTGCAAATTCTCTAAACTTGTGTTTATCTGTAAATATCTTAATTTCTCCTTCFFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFF:FFFFF,FFFFFFFFFF:FFFFFFFF,FF,FF:FFFFFFF::FF,FFF::FF,,FFFFFFFFFFFFFFFFFFFFF,F::FF,F,F:FFNM:i:1MD:Z:70A80MC:Z:124M27SAS:i:146XS:i:23Yt:Z:NR 0 60 CGTTCTGTTTTGTGACCTTGTATTGTGCTGGTACCTGATATTATTGGTCATCAGGCCAAACAATCTCCTTTAGCATTTCTTGCAGTCTTGGTTTAGTTTTTGCAAATTCTCTAAACTTGTGTTTATCTGTAAATATCTTAATTTCTCCTTC +A00126:224:H7HMWDSX3:3:1102:15085:10864 Test_seq 365724 Test_seq 366184 + - UU A00126:224:H7HMWDSX3:3:1102:15085:10864161Test_seq36572460151M=366034461AAATGAAATTCCACTTGTATTTTACTGCACACTCATAAAACAAGAAAAACACATGAGTTGTTTTTAAAGCCAATGAAATGCAGAGATTATCAAACAATATCGTTAATATGACACGCAAGTAAAATTATACTGAAGATCACTCAAAAACGGTFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFF,F:FFFFFFF:FFFFFFF:F:FFFFFF:FFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFF,FFFFFFFFFFFF:FFFF,FFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1102:15085:1086481Test_seq36603460151M=365724-461TTAACAAATTATGGATAACATTGGGAAGAATGGGAATTCCAGAACAATTAATTGTGCTCATAAGAACCTGGACATAGACCACGAGGCAGTCATTCGAACATGACAAGGGTATACTTTGTGGTTTAAAATCAGGAAAGGGGTTCCTCCAAGTFFFFFF,FF:FFFFF::F:F,FFFFFFFFFFFFFFFFFFFFF:FFFFF:FF:FFFFFFF,FF:F:FFFFFFFFF,FFFFFFFFFFFFFFFFFFFF:F:FF,FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU 60 60 AAATGAAATTCCACTTGTATTTTACTGCACACTCATAAAACAAGAAAAACACATGAGTTGTTTTTAAAGCCAATGAAATGCAGAGATTATCAAACAATATCGTTAATATGACACGCAAGTAAAATTATACTGAAGATCACTCAAAAACGGT TTAACAAATTATGGATAACATTGGGAAGAATGGGAATTCCAGAACAATTAATTGTGCTCATAAGAACCTGGACATAGACCACGAGGCAGTCATTCGAACATGACAAGGGTATACTTTGTGGTTTAAAATCAGGAAAGGGGTTCCTCCAAGT +A00126:224:H7HMWDSX3:3:1102:7925:11616 ! 0 Test_seq 168515 - + NR A00126:224:H7HMWDSX3:3:1102:7925:1161681Test_seq16855460122M29S=168515-161TTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTGTTGATAAGATCTGAGTTTTATTTTACTAAACATTATCCAC:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:110A11MC:Z:151MAS:i:117XS:i:71Yt:Z:NR A00126:224:H7HMWDSX3:3:1102:7925:11616161Test_seq16851560151M=168554161TCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTGTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:149A1MC:Z:122M29SAS:i:149XS:i:74Yt:Z:NR 0 60 TCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTGT +A00126:224:H7HMWDSX3:3:1102:31575:12649 Test_seq 213195 Test_seq 213257 + - UU A00126:224:H7HMWDSX3:3:1102:31575:12649161Test_seq2131951263M=21319563CCTGATCTCAGAACCTATTATACAGCTACAGTAGTCAAAACAGCCTGGTACTGGTACAACAACFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFF:FNM:i:1MD:Z:6T56MC:Z:63MAS:i:58XS:i:53XA:Z:Test_seq,-167781,63M,2;Yt:Z:UU A00126:224:H7HMWDSX3:3:1102:31575:1264981Test_seq2131951063M=213195-63CCTGATTTCAGAACCTATTATACAGCCACAGTAGTCAAAACAGCCTGGTACTGGTACAACAACFFFFFFFFFFFFFFFFFFF::,FFFFFFFFF:FFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:26T36MC:Z:63MAS:i:58XS:i:53XA:Z:Test_seq,+167781,63M,2;Test_seq,+152050,63M,3;Yt:Z:UU 12 10 CCTGATCTCAGAACCTATTATACAGCTACAGTAGTCAAAACAGCCTGGTACTGGTACAACAAC CCTGATTTCAGAACCTATTATACAGCCACAGTAGTCAAAACAGCCTGGTACTGGTACAACAAC +A00126:224:H7HMWDSX3:3:1102:20039:13150 ! 0 Test_seq 167411 - + MU A00126:224:H7HMWDSX3:3:1102:20039:1315065Test_seq213570054M=167411-46160CCAAAAAAGACGAAGAGAAACCAGATAACTGGGAGCTCCTAAAAAACAAACACC,:FFF::,FF,F:FF:F:F,FFFFFFF,F,F,,F,,:,FF,FF,F,:FF,FF:FNM:i:3MD:Z:7C2T34T8MC:Z:54MAS:i:39XS:i:39XA:Z:Test_seq,-167411,54M,3;Yt:Z:MU A00126:224:H7HMWDSX3:3:1102:20039:13150129Test_seq1674111354M=21357046160GGTGTTTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTCGTCATTTTTGGFFF,FFFFFF:FFFFFFFFF::FFF,FFFFFFFF,F::FF:,,FFF:F,FFFF:NM:i:1MD:Z:43A10MC:Z:54MAS:i:49XS:i:44XA:Z:Test_seq,-213570,54M,2;Yt:Z:MU 0 13 CCAAAAAAGACGAAGAGAAACCAGATAACTGGGAGCTCCTAAAAAACAAACACC GGTGTTTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTCGTCATTTTTGG +A00126:224:H7HMWDSX3:3:1102:18783:13855 Test_seq 188880 Test_seq 189219 + - UU A00126:224:H7HMWDSX3:3:1102:18783:13855161Test_seq18888060151M=189069340AATTCTGTTAGGACCTAAGTTTTAGATTACCTGTGAGCCTTTTTTCACTCAGAATAAAAAACTCATTCTGAGGATAAGTTCAAAGAGACTGAATGTTCAAATGTAATTCACCACCAACCATGTAAGGTGGCTTATTTCATGAATTTTTGAAFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF,FNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1102:18783:1385581Test_seq18906960151M=188880-340CTTAACTTGAGAACCTCTTTGATAAATTTTTACAAAAGATCCATGGAGAAACAAAGACGTTTGTTTTAGTGATTGTGAAAGATGATGTAGGTATCTATAAAAAATGTAATTTAACTAGTGTCCACTCTTTGCCCTAAATTATATTCCTAAC,FFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU 60 60 AATTCTGTTAGGACCTAAGTTTTAGATTACCTGTGAGCCTTTTTTCACTCAGAATAAAAAACTCATTCTGAGGATAAGTTCAAAGAGACTGAATGTTCAAATGTAATTCACCACCAACCATGTAAGGTGGCTTATTTCATGAATTTTTGAA CTTAACTTGAGAACCTCTTTGATAAATTTTTACAAAAGATCCATGGAGAAACAAAGACGTTTGTTTTAGTGATTGTGAAAGATGATGTAGGTATCTATAAAAAATGTAATTTAACTAGTGTCCACTCTTTGCCCTAAATTATATTCCTAAC +A00126:224:H7HMWDSX3:3:1102:27326:13839 Test_seq 99580 Test_seq 99923 + - UU A00126:224:H7HMWDSX3:3:1102:27326:13839161Test_seq995806015S136M=99773344ACCCAAGTTATGATCGATCTATTCCTGTGAAGACCACAGTCTAGAAAACCCTGTGGGGCAGTTCTGCTCTGTCACATGGGTTCACTGAGCAGGAATCACCTTGAGGATACCTAACAGAGCAAAAACAAGGAGTTACTGGTAAAAACCTGCAFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:136MC:Z:151MAS:i:136XS:i:28Yt:Z:UU A00126:224:H7HMWDSX3:3:1102:27326:1383981Test_seq9977360151M=99580-344AGTGCGGCCCCTCCTCATCACTATGCCCATGCTTATGGCCGAGCTGGGTGTCATGGGTCAGGAAGATGCTGGAGAGGGAAGTGACCCAGCGATGGCCTTCACAGGAAGCCAGAGTCCAGCAGCCAAGATCAAGAGTCCAGTTGGCCAGTGC:FFFFFFFF:FFFFFFFFFFFFFFFFFF:FFFFFF,FFFFFFF:FF:FFFFF,FFFFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFF:FFFFFFFFFF,FFFFF:FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:15S136MAS:i:151XS:i:0Yt:Z:UU 60 60 ACCCAAGTTATGATCGATCTATTCCTGTGAAGACCACAGTCTAGAAAACCCTGTGGGGCAGTTCTGCTCTGTCACATGGGTTCACTGAGCAGGAATCACCTTGAGGATACCTAACAGAGCAAAAACAAGGAGTTACTGGTAAAAACCTGCA AGTGCGGCCCCTCCTCATCACTATGCCCATGCTTATGGCCGAGCTGGGTGTCATGGGTCAGGAAGATGCTGGAGAGGGAAGTGACCCAGCGATGGCCTTCACAGGAAGCCAGAGTCCAGCAGCCAAGATCAAGAGTCCAGTTGGCCAGTGC +A00126:224:H7HMWDSX3:3:1102:13756:14732 Test_seq 169110 Test_seq 169462 + - UU A00126:224:H7HMWDSX3:3:1102:13756:1473297Test_seq16911035151M=169312353ATTTAGTTGTTCTACTTCTGAATGTGTTAGTTTAGGTAGGTAGTGTTTTTCCAGGAATTCATCCATTTCTTCTAGGTTTTCAAATTTGTTAGAGTACAATTTTTCATAATAATCTGAAATGATTCTTTGAATTTCATTTGGTTCTGTTGTGFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFF:FFFFFF:FFFFFFFF,FFFNM:i:8MD:Z:51G1A37C11A1G2G19T12C9MC:Z:151MAS:i:111XS:i:91XA:Z:Test_seq,+153408,136M15S,9;Yt:Z:UU A00126:224:H7HMWDSX3:3:1102:13756:14732145Test_seq16931260151M=169110-353TCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTGATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:38A112MC:Z:151MAS:i:146XS:i:107Yt:Z:UU 35 60 ATTTAGTTGTTCTACTTCTGAATGTGTTAGTTTAGGTAGGTAGTGTTTTTCCAGGAATTCATCCATTTCTTCTAGGTTTTCAAATTTGTTAGAGTACAATTTTTCATAATAATCTGAAATGATTCTTTGAATTTCATTTGGTTCTGTTGTG TCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTGATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTG +A00126:224:H7HMWDSX3:3:1102:28845:17159 ! 0 Test_seq 168418 - + NR A00126:224:H7HMWDSX3:3:1102:28845:17159145Test_seq16868860123M28S=168418-393TCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCACACCTGGTATAAATCCCACTGGATAAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTAGTCCTGGTGGCATAATAGTTAAGCACTFFFFFFFFFFFF:F:FFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFNM:i:4MD:Z:28T35T19T3C34MC:Z:151MAS:i:103XS:i:49Yt:Z:NR A00126:224:H7HMWDSX3:3:1102:28845:1715997Test_seq16841860151M=168688393AGCCTAATTGCCCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTFFFF:,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFF:,FFFFFFFFFF:FFFFFFFFFFFFFFFFFF,FFFFFFF,NM:i:1MD:Z:77T73MC:Z:123M28SAS:i:146XS:i:74Yt:Z:NR 0 60 AGCCTAATTGCCCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCT +A00126:224:H7HMWDSX3:3:1102:10881:18176 Test_seq 169339 Test_seq 169775 + - UU A00126:224:H7HMWDSX3:3:1102:10881:18176161Test_seq16933960151M=169625437TCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGATTCTTTTGTTGCTCACTTTCTAFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FF,FFF:FFFFFFFFF,FFFFFFFFFFFFFFF,,FF:FFFFF,FFF,FFFFF::FFFFFFFFFNM:i:1MD:Z:128G22MC:Z:151MAS:i:146XS:i:103Yt:Z:UU A00126:224:H7HMWDSX3:3:1102:10881:1817681Test_seq16962560151M=169339-437TCTCGTTGCTTTCTATGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTATTGTTCATTTTCCAAGTATTTGATTTCTTTTCCCTCGTTTTTCTGTTATTGATCTCTAGTTTTATTGCCTTGTGATCFFFFFFFF:FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:9MD:Z:3T11A22G70A12C4T5C2C11G2MC:Z:151MAS:i:109XS:i:0Yt:Z:UU 60 60 TCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGATTCTTTTGTTGCTCACTTTCTA TCTCGTTGCTTTCTATGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTATTGTTCATTTTCCAAGTATTTGATTTCTTTTCCCTCGTTTTTCTGTTATTGATCTCTAGTTTTATTGCCTTGTGATC +A00126:224:H7HMWDSX3:3:1102:21757:18286 Test_seq 168369 Test_seq 168782 + - UU A00126:224:H7HMWDSX3:3:1102:21757:18286161Test_seq16836960151M=168632414TAGCTTTACTTCCTCCTTACCAATCTGGATGATCTTTATTTCTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGTGGTGATAAAGGGCATCCTTGTCCGGTTCCCGTTCTCGAGGGAAGTGCTTTCAGGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FF:FFFF:FFFFFF:FFFFFFFFFFFFFFFF,FFFFFFFFFFF:FFFF,FFFF:FFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFNM:i:9MD:Z:18G11A0C0C63C22T6T6A6A10MC:Z:151MAS:i:106XS:i:49Yt:Z:UU A00126:224:H7HMWDSX3:3:1102:21757:1828681Test_seq16863260151M=168369-414GTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:84T66MC:Z:151MAS:i:146XS:i:66Yt:Z:UU 60 60 TAGCTTTACTTCCTCCTTACCAATCTGGATGATCTTTATTTCTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGTGGTGATAAAGGGCATCCTTGTCCGGTTCCCGTTCTCGAGGGAAGTGCTTTCAGG GTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTG +A00126:224:H7HMWDSX3:3:1102:22073:20713 Test_seq 167953 Test_seq 168325 + - UU A00126:224:H7HMWDSX3:3:1102:22073:20713161Test_seq16795360151M=168175373TATGACATTGGTATTTGGATCGGAAGTGCGTTATATGTATAGATGGCTTTTGGTAGAATAGACATTTTTACTATGTTAAGTCTTCCTATCCATGAGCAGGGTATGTTCTTCCACTTAAGTATGTCCTTTTGAATTTCTTGTAGCAGAGTTTFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:NM:i:1MD:Z:20T130MC:Z:151MAS:i:146XS:i:77Yt:Z:UU A00126:224:H7HMWDSX3:3:1102:22073:2071381Test_seq1681757151M=167953-373GCTACTGTGAACGGTATTGATTTGGTTATTTCCTCTTCGGTGTTCTTTTTGTTGATGTAGAGGAATCCAAGTGATTTTTGTATGTTTATTTTATAACCTGAGACTCTGCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCTFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:3MD:Z:11T84T10T43MC:Z:151MAS:i:136XS:i:131XA:Z:Test_seq,+212713,151M,4;Yt:Z:UU 60 7 TATGACATTGGTATTTGGATCGGAAGTGCGTTATATGTATAGATGGCTTTTGGTAGAATAGACATTTTTACTATGTTAAGTCTTCCTATCCATGAGCAGGGTATGTTCTTCCACTTAAGTATGTCCTTTTGAATTTCTTGTAGCAGAGTTT GCTACTGTGAACGGTATTGATTTGGTTATTTCCTCTTCGGTGTTCTTTTTGTTGATGTAGAGGAATCCAAGTGATTTTTGTATGTTTATTTTATAACCTGAGACTCTGCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCT +A00126:224:H7HMWDSX3:3:1102:18159:21324 ! 0 Test_seq 171187 - + NR A00126:224:H7HMWDSX3:3:1102:18159:2132481Test_seq17132760129M22S=171187-269TCCTTTCGTCTTTCATGATGTCAGGGAAGTTTTGTGTCAGGAGTTCTTCAACTATTTTCTCTGTGTTTTCTGTCCCCCCTCCCTGTTCTGGGACTCCAATCACTCGCAAGTTATCCTTCTTGATAGAGTAATCTTTTCCCATCCAAGAACTFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:2MD:Z:73G15T39MC:Z:151MAS:i:119XS:i:0Yt:Z:NR A00126:224:H7HMWDSX3:3:1102:18159:21324161Test_seq17118760151M=171327269GGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATGGGGTTCGATGAGCATCTTGGATAGATATCCTTTCGTCTFFFFFFFFFFFF:::FFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF,F:FFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFF,FFFFFF:FFFFFF:F:FFFFFF:FFNM:i:0MD:Z:151MC:Z:129M22SAS:i:151XS:i:72Yt:Z:NR 0 60 GGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATGGGGTTCGATGAGCATCTTGGATAGATATCCTTTCGTCT +A00126:224:H7HMWDSX3:3:1102:32226:21762 Test_seq 170191 Test_seq 170652 + - UU A00126:224:H7HMWDSX3:3:1102:32226:2176297Test_seq1701916085M1D66M=170502462GTGGTGGATTTAAGTCTAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCCTCTTCTTTTTTGCTTATTATTTGCTTGATATATTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTCAGTCTAAGGTGTGTCTCTTGTAGGCAFFFFFFFFFFFFFFFFFFFFFFF:F,FFFFFFFFF::FFFFFFFFF,F,F,FFFF,FFF,FFFFFF,F::FFFFFFFF:FFFFFFFFFFFFFFFFFFF,FFFF,,F:FFFFFFF:FFFFFFFFF,FFFFF:FFFFFFF:F,F,FFFFFFF:NM:i:4MD:Z:4C0A79^T39A26MC:Z:151MAS:i:133XS:i:73Yt:Z:UU A00126:224:H7HMWDSX3:3:1102:32226:21762145Test_seq17050260151M=170191-462CACATACTTTTTTGTGCTGAGACGTTTTTCTTAGTAGATTGTGAGATCCTCATTTTCATAATGTTTAACTTTATGTTTGTTGAGTCATTACGTTTTTCTTGAGTTATGGAATTGATATTCCTTTTTGTGGTTACCTTATTATTTACCCCTAF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFF,FFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFF:FFF::FF:FF:FFFFFF:FNM:i:1MD:Z:86G64MC:Z:85M1D66MAS:i:146XS:i:28Yt:Z:UU 60 60 GTGGTGGATTTAAGTCTAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCCTCTTCTTTTTTGCTTATTATTTGCTTGATATATTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTCAGTCTAAGGTGTGTCTCTTGTAGGCA CACATACTTTTTTGTGCTGAGACGTTTTTCTTAGTAGATTGTGAGATCCTCATTTTCATAATGTTTAACTTTATGTTTGTTGAGTCATTACGTTTTTCTTGAGTTATGGAATTGATATTCCTTTTTGTGGTTACCTTATTATTTACCCCTA +A00126:224:H7HMWDSX3:3:1102:26648:21903 ! 0 Test_seq 168607 - - MU A00126:224:H7HMWDSX3:3:1102:26648:21903177Test_seq2127280149M2S=168457-44270AACTACTGAAACTAATAGAAGAGTTTGTCAGAGTCTCAGGTTATAAAATAAACATACAAAAATCACTTGGATTCCTCTACATCAACAAAAAGAACACCGAAGAGGAAATAATCAAATCAATACCATTCACAGTAGCCCCCAAGAAGATACTF::FFFF,:FFF:F:F:,F:,F:FFFF,FF:FFFFFFF,FFFFFFFFFFF:FF:FFFFF:::FFFFFFFFF:FFFFFFF:FF,F:FF,FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF,FFF:FFFFFF:F:FFFFFFFNM:i:5MD:Z:27G18G49T1A12C37MC:Z:151MAS:i:124XS:i:124XA:Z:Test_seq,+168162,2S149M,5;Yt:Z:MU A00126:224:H7HMWDSX3:3:1102:26648:21903113Test_seq16845760151M=21272844270AATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGFFFF:FFFFFFF:FFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:FFF,:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFNM:i:1MD:Z:38T112MC:Z:149M2SAS:i:146XS:i:91Yt:Z:MU 0 60 AACTACTGAAACTAATAGAAGAGTTTGTCAGAGTCTCAGGTTATAAAATAAACATACAAAAATCACTTGGATTCCTCTACATCAACAAAAAGAACACCGAAGAGGAAATAATCAAATCAATACCATTCACAGTAGCCCCCAAGAAGATACT AATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGG +A00126:224:H7HMWDSX3:3:1102:9109:22592 Test_seq 168345 Test_seq 168707 + - UU A00126:224:H7HMWDSX3:3:1102:9109:2259297Test_seq16834560151M=168557363GATCATGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCTTGCCAATCCGGATACCCTTTATTTCTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGACATCCTTGTCTGGTTCCCFFFFFFF:FFFFFFFFFFFFFFF:FFFFFF:FFFFFF:FFFFFFFFFF:F::FFFFFF:FFFFFFFFFFFFF,FFFFFFFFF:F:FFF:FFFFFFFF,:FF:F:FFFFFFF:FF:FF,FF,F:FFFFFF:F:FFFF::FFFF,F,,F:FFFNM:i:5MD:Z:1G18A28T82G17T0MC:Z:151MAS:i:133XS:i:76Yt:Z:UU A00126:224:H7HMWDSX3:3:1102:9109:22592145Test_seq16855760151M=168345-363CATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCAATTGATAAGATCATGTGGTTTTTATCTTTTGTTTTATTTATGTGFFFFFFFFF::FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:106T44MC:Z:151MAS:i:146XS:i:87Yt:Z:UU 60 60 GATCATGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCTTGCCAATCCGGATACCCTTTATTTCTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGACATCCTTGTCTGGTTCCC CATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCAATTGATAAGATCATGTGGTTTTTATCTTTTGTTTTATTTATGTG +A00126:224:H7HMWDSX3:3:1102:26431:27759 Test_seq 129405 Test_seq 129790 + - UU A00126:224:H7HMWDSX3:3:1102:26431:27759161Test_seq12940560151M=129640386GTACTTGAATCTGCTCAGCTCTTAATGGTTACAACTGTTAACCCACAATGTCAGAATGATTTTCACACTTGTAAATCCCTAGAACTTTAATCTGGCTGCAAAAGAAGTCCTAGGCTCTTTCATTTAGTGGTAACGATGGTGGCAGGCACCTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1102:26431:2775981Test_seq12964060151M=129405-386CATCTTGACTCCTGCAGGTCACAAGAATAGTACAGGGATAGAGCATGCATTCCTAAGCCTGCCCTTCAACTGAACAGGAAATGGCAACTCTGGTCAAGAGTTTGTCCCCAAATGCCACTACAGTGTGCAGCAATGTTTCCTCTGGGCTGTGFFFFFFFFFFFFFFFFFF,FFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU 60 60 GTACTTGAATCTGCTCAGCTCTTAATGGTTACAACTGTTAACCCACAATGTCAGAATGATTTTCACACTTGTAAATCCCTAGAACTTTAATCTGGCTGCAAAAGAAGTCCTAGGCTCTTTCATTTAGTGGTAACGATGGTGGCAGGCACCT CATCTTGACTCCTGCAGGTCACAAGAATAGTACAGGGATAGAGCATGCATTCCTAAGCCTGCCCTTCAACTGAACAGGAAATGGCAACTCTGGTCAAGAGTTTGTCCCCAAATGCCACTACAGTGTGCAGCAATGTTTCCTCTGGGCTGTG +A00126:224:H7HMWDSX3:3:1102:27534:28792 Test_seq 168155 Test_seq 168644 + - UU A00126:224:H7HMWDSX3:3:1102:27534:28792161Test_seq16815518151M=168494490AGTATTTTATCTTCTTGGGGGCTACTGTGAATGGTATTGATTTGGTTATATCCTCTTCGGTATTCTTTTTGTTGATGTAGAGGAATCCAAGTGATTTTTATATGTTTATTTTATATCCTGAGACACTTCCAAACTCTTCTATTAGTTTCAGFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF:FFFFF:FFFFFF:F::F:FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FNM:i:7MD:Z:14C34T11G37G15A0T7T26MC:Z:151MAS:i:116XS:i:106XA:Z:Test_seq,-212733,151M,9;Yt:Z:UU A00126:224:H7HMWDSX3:3:1102:27534:2879281Test_seq16849460151M=168155-490CCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCFFFFFFFFFFFFFF,FFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:1T149MC:Z:151MAS:i:149XS:i:96Yt:Z:UU 18 60 AGTATTTTATCTTCTTGGGGGCTACTGTGAATGGTATTGATTTGGTTATATCCTCTTCGGTATTCTTTTTGTTGATGTAGAGGAATCCAAGTGATTTTTATATGTTTATTTTATATCCTGAGACACTTCCAAACTCTTCTATTAGTTTCAG CCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTC +A00126:224:H7HMWDSX3:3:1102:24433:32565 Test_seq 169299 Test_seq 169714 + - UU A00126:224:H7HMWDSX3:3:1102:24433:3256597Test_seq16929960151M=169564416CCTTTCCTGTATTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFNM:i:1MD:Z:10T140MC:Z:151MAS:i:146XS:i:106Yt:Z:UU A00126:224:H7HMWDSX3:3:1102:24433:32565145Test_seq16956460151M=169299-416AATTGACCTCTGAGCACTGCTTTTGCTGTGTCCCAGAGGTTTTGATAGGAAGTATTTTCATTCTCGTTGCTGTCTATGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTATTGTTCATTTTCCAAGFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF,FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:5MD:Z:5G58T6T4A22G51MC:Z:151MAS:i:126XS:i:66Yt:Z:UU 60 60 CCTTTCCTGTATTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTT AATTGACCTCTGAGCACTGCTTTTGCTGTGTCCCAGAGGTTTTGATAGGAAGTATTTTCATTCTCGTTGCTGTCTATGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTATTGTTCATTTTCCAAG +A00126:224:H7HMWDSX3:3:1102:24343:32910 Test_seq 169299 Test_seq 169714 + - UU A00126:224:H7HMWDSX3:3:1102:24343:3291097Test_seq16929960151M=169564416CCTTTCCTGTATTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTFFFFFF::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF,FFF,FFFFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFNM:i:1MD:Z:10T140MC:Z:151MAS:i:146XS:i:106Yt:Z:UU A00126:224:H7HMWDSX3:3:1102:24343:32910145Test_seq16956460151M=169299-416AATTGACCTCTGAGCACTGCTTTTGCTGTGTCCCAGAGGTTTTGATAGGAAGTATTTTCATTCTCGTTGCTGTCTATGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTATTGTTCATTTTCCAAGFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:5MD:Z:5G58T6T4A22G51MC:Z:151MAS:i:126XS:i:66Yt:Z:UU 60 60 CCTTTCCTGTATTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTT AATTGACCTCTGAGCACTGCTTTTGCTGTGTCCCAGAGGTTTTGATAGGAAGTATTTTCATTCTCGTTGCTGTCTATGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTATTGTTCATTTTCCAAG +A00126:224:H7HMWDSX3:3:1102:24379:34413 Test_seq 169290 Test_seq 169639 + - UU A00126:224:H7HMWDSX3:3:1102:24379:3441397Test_seq16929060151M=169489350TATTTGTTTCCTTTCCTGTTTTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:102Yt:Z:UU A00126:224:H7HMWDSX3:3:1102:24379:34413145Test_seq16948960151M=169290-350ATTTGTTCAAGTTGTCAGGACAGTTCTCTGATTTTGGCTCTTTCTTCTTTTTGTATGTGTGCATTTATCGATAAAAATTGGCCTCTGAGCACTGCTTTTGCTGTGTCCCAGAGGTTTTGATAGGAAGTATTTTCATTCTCGTTGCTTTCTAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:4MD:Z:30C0C41T65T11MC:Z:151MAS:i:131XS:i:70Yt:Z:UU 60 60 TATTTGTTTCCTTTCCTGTTTTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATT ATTTGTTCAAGTTGTCAGGACAGTTCTCTGATTTTGGCTCTTTCTTCTTTTTGTATGTGTGCATTTATCGATAAAAATTGGCCTCTGAGCACTGCTTTTGCTGTGTCCCAGAGGTTTTGATAGGAAGTATTTTCATTCTCGTTGCTTTCTA +A00126:224:H7HMWDSX3:3:1102:2031:34757 ! 0 Test_seq 167704 - - NR A00126:224:H7HMWDSX3:3:1102:2031:34757177Test_seq2135927127M24S=167554-46015CGATAACTGGTATCTCCTAAAAATCAAACACCTATGCTCATCTAAAGACTTCACCAAAAGAGTAAAAAGACCACCTACAGACTGTGAAAGAATTTTCAGCTATGACATCTCAGACCAGCGCCTGATCGATCTCAAACTTTTGCCTAGTAGAF,:FFF,F,,,F,F::FFFFFFF,FFFFFF,F,FF:,F:F,F,F:F,FFF:FFFFFFFF:FF:FFFFF::,:F::FF:F,F,F,,:FFF:FF:FF:,F,F,:F,FFFF,FFF,FFFFFFFF,FFFF,FFF,F,FFF,FFFF,F:FF::F:FNM:i:6MD:Z:0A9G1G64T6G26C15MC:Z:151MAS:i:101XS:i:96XA:Z:Test_seq,+167316,24S127M,7;Test_seq,+400737,24S114M13S,5;Yt:Z:NR A00126:224:H7HMWDSX3:3:1102:2031:34757113Test_seq16755431151M=21359246015ATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATCATTTCTCCAATTGACTGACACTGGTCCTTFFF:FFFFFF,:FF:FF:,FFFF:FFFFFF,:,FFF:FFFFFFFFF::F:,FFFFFFFF:F,F:F,FFFF,:FFF:F:FFF:FFFFF:FFF:FF,,FF:FF:,FFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFF,FFF,FFFFF,FFNM:i:1MD:Z:127C23MC:Z:127M24SAS:i:146XS:i:131XA:Z:Test_seq,+213334,126M2I23M,4;Yt:Z:NR 0 31 ATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATCATTTCTCCAATTGACTGACACTGGTCCTT +A00126:224:H7HMWDSX3:3:1102:12563:35650 Test_seq 346775 Test_seq 347269 + - UU A00126:224:H7HMWDSX3:3:1102:12563:35650161Test_seq34677560151M=347119495GTTTCTTAGACATGGAAACCCTGGTGGTGTAGTGGTTAAGTGCTACGGGGCTGCTAACCAAGAGGTCGGCATTTCGAATCCACCAGGCACTCCTTGGAAACTCTATGGGGCAGTTCTACTCTGTCCTATAGGGTCGCTATGAGTCGGAATTFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFF:FFFF:F:FFFFFFFFF,FF:F,FF,FFF,FNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:77Yt:Z:UU A00126:224:H7HMWDSX3:3:1102:12563:3565081Test_seq34711960151M=346775-495ATATAAACCAAAAAATAGATAAGATAGAAAGTATTAGGCTAAGTTATGGAGAGATTTTTGAGGAGTTTTTTTTTTTTAATTTTTATTGTGCTTGAAGTGAAAGTTTACAAATCAAGTCAGTCTCTCATAGAAAAATTTATATACACCTTGCFFF:FFFFF:FFFF:F::FF::FFFF,FF::FF:FF,,FF:F:FFFF:FF:FFFFFFFFFF:FF::FFFFFF::FFFFFFFFF,FFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF,FFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:59Yt:Z:UU 60 60 GTTTCTTAGACATGGAAACCCTGGTGGTGTAGTGGTTAAGTGCTACGGGGCTGCTAACCAAGAGGTCGGCATTTCGAATCCACCAGGCACTCCTTGGAAACTCTATGGGGCAGTTCTACTCTGTCCTATAGGGTCGCTATGAGTCGGAATT ATATAAACCAAAAAATAGATAAGATAGAAAGTATTAGGCTAAGTTATGGAGAGATTTTTGAGGAGTTTTTTTTTTTTAATTTTTATTGTGCTTGAAGTGAAAGTTTACAAATCAAGTCAGTCTCTCATAGAAAAATTTATATACACCTTGC +A00126:224:H7HMWDSX3:3:1103:19000:1892 ! 0 Test_seq 169431 - - MR A00126:224:H7HMWDSX3:3:1103:19000:18922209Test_seq1689966028H123M=169281436TAAGTCTTCTCTGAAGGTTTGGTAGAACTCTGCAGTGAAGCCGTCTGGGCCAGGACTTTTTTTTGTTGGAAGTTTTTTGATTACCGTTTCAATCTCGTTTTTTTTTATGGGTCTATTTAGTTGFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFF,FFF:FFFF:FF:FFFFFFFFFFFFFF:FFF,FFF:FFFFFFFFFFFFFFFNM:i:2MD:Z:96T6G19MC:Z:151MAS:i:113XS:i:36SA:Z:Test_seq,400060,-,122S29M,0,1;Yt:Z:MRNEXT_SAMA00126:224:H7HMWDSX3:3:1103:19000:1892177Test_seq4000600122S29M=169281-230658CAACTAAATAGACCCATAAAAAAAAACGAGATTGAAACGGTAATCAAAAAACTTCCAACAAAAAAAAGTCCTGGCCCAGACGGCTTCACTGCAGAGTTCTACCAAACCTTCAGAGAAGACTTAGATCTTATAATAGTGGTCTTATACAGTAFFFFFFFFFFFFFFF:FFF,FFF:FFFFFFFFFFFFFF:FF:FFFF:FFF,FFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:26A2MC:Z:151MAS:i:26XS:i:26SA:Z:Test_seq,168996,+,28S123M,60,2;XA:Z:Test_seq,-166639,122S29M,1;Test_seq,+214367,18M1I10M122S,1;Yt:Z:MR A00126:224:H7HMWDSX3:3:1103:19000:1892113Test_seq16928160151M=400060230658TATTCGGGTTATTTGTTTCCTTTCCTGTATTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:28T122MC:Z:122S29MAS:i:146XS:i:103Yt:Z:MR 0 60 CAACTAAATAGACCCATAAAAAAAAACGAGATTGAAACGGTAATCAAAAAACTTCCAACAAAAAAAAGTCCTGGCCCAGACGGCTTCACTGCAGAGTTCTACCAAACCTTCAGAGAAGACTTAGATCTTATAATAGTGGTCTTATACAGTA TATTCGGGTTATTTGTTTCCTTTCCTGTATTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTA +A00126:224:H7HMWDSX3:3:1103:30544:2378 ! 0 Test_seq 168612 - - MU A00126:224:H7HMWDSX3:3:1103:30544:2378161Test_seq1682780151M=168462335CTCTGCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGGTTTTCTGTGTATATAATCATGTCATCTGCAAATAGTGTTAGCTTTACTTCCTCCTTGCCAATCCGGATACCTTTTATTTCTTTGTCTAGCGTAATTGTFFFF:,FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:,FFFFFFFFFFFFFFFFFF:FFFFFFFFF:F,:FFF:,FNM:i:12MD:Z:4T53C0A6C0G0G18A2A25T7C18C6C0MC:Z:151MAS:i:95XS:i:95XA:Z:Test_seq,-212610,151M,12;Yt:Z:MU A00126:224:H7HMWDSX3:3:1103:30544:237881Test_seq16846260151M=168278-335GAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAFFFFFFFFFFF,FFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFNM:i:1MD:Z:33T117MC:Z:151MAS:i:146XS:i:91Yt:Z:MU 0 60 CTCTGCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGGTTTTCTGTGTATATAATCATGTCATCTGCAAATAGTGTTAGCTTTACTTCCTCCTTGCCAATCCGGATACCTTTTATTTCTTTGTCTAGCGTAATTGT GAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGA +A00126:224:H7HMWDSX3:3:1103:25880:3098 Test_seq 183557 Test_seq 184047 + - UU A00126:224:H7HMWDSX3:3:1103:25880:3098161Test_seq18355760151M=183897491ATCCAGTTGGTTCCGACTCATAGCATCCCTATGTACAACAGAATGAAACGCTGCCCTGTCCTGTGCCAGCCTTGCAATCATTGTTATGCTTGATCCCATTGTTGTAGCCACTGTATCAATCCATCTCATTGAGGGTCTTGCTCTTTTTCACFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFF:FFFFFFFFF:FFF:FFFFFFFF:FFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:30Yt:Z:UU A00126:224:H7HMWDSX3:3:1103:25880:309881Test_seq18389760151M=183557-491TCAATTCTCCGGTCTTTCTTAGTCATTGTTCAGCTTTCAAATGCATATTATGCGATTGAAAACACCATGGTTTGGGTCAGGTGCCCCTTAGTCCTCAAAGTGACATCTTTGCTTTTTAACACTTTAAAGAGGTCTTTTGCAGCAGATTTACFFFF:FFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:64Yt:Z:UU 60 60 ATCCAGTTGGTTCCGACTCATAGCATCCCTATGTACAACAGAATGAAACGCTGCCCTGTCCTGTGCCAGCCTTGCAATCATTGTTATGCTTGATCCCATTGTTGTAGCCACTGTATCAATCCATCTCATTGAGGGTCTTGCTCTTTTTCAC TCAATTCTCCGGTCTTTCTTAGTCATTGTTCAGCTTTCAAATGCATATTATGCGATTGAAAACACCATGGTTTGGGTCAGGTGCCCCTTAGTCCTCAAAGTGACATCTTTGCTTTTTAACACTTTAAAGAGGTCTTTTGCAGCAGATTTAC +A00126:224:H7HMWDSX3:3:1103:26639:3818 Test_seq 41455 Test_seq 214212 + - UU A00126:224:H7HMWDSX3:3:1103:26639:381897Test_seq414556151M=214062172758TGGGCAAAGGATATGAACAGGCACTTCACTAAAGAAGACATTCAGGTAGCTAACAGATACATGAGGAAATGCTCATGATCATTAGCCATCAGAGAAATGCAAATCAAAACTATGACGAGATACCATTTCACCCCAACAAGGCTGGCATTAA:FFF:F:FFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:F:FFFFFFFFFFFFFFFF:FF:FFFF:FFFFFF::FFFFFF:FFFFFFF,F:FNM:i:13MD:Z:20A25C0G26T0C13T22C0A1T5T4C4T15C3MC:Z:151MAS:i:87XS:i:81XA:Z:Test_seq,-49064,151M,14;Test_seq,-151371,151M,15;Test_seq,+258709,151M,16;Yt:Z:UU A00126:224:H7HMWDSX3:3:1103:26639:3818145Test_seq21406260151M=41455-172758AAATAGAACTACCATACAACCCAGAAATCCCACTCCTCGGAATATACCCTAGAGAAATAAGAGCCTTCACACAAACAGATATATGCACACCCATGTTTATTGCAGCTCTGTTTACAATAGCAAAAAGCTGGAAGCAACCAAGGTGTCCGTCF,FFFFFF:FFF,FFFFFFFFFF:FF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF,FFFFFFFFFF:FFFF,FFFFF:FFFFF,FFFFFFFF:FFFFFFFFFFFFFF::FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:148A2MC:Z:151MAS:i:148XS:i:116Yt:Z:UU 6 60 TGGGCAAAGGATATGAACAGGCACTTCACTAAAGAAGACATTCAGGTAGCTAACAGATACATGAGGAAATGCTCATGATCATTAGCCATCAGAGAAATGCAAATCAAAACTATGACGAGATACCATTTCACCCCAACAAGGCTGGCATTAA AAATAGAACTACCATACAACCCAGAAATCCCACTCCTCGGAATATACCCTAGAGAAATAAGAGCCTTCACACAAACAGATATATGCACACCCATGTTTATTGCAGCTCTGTTTACAATAGCAAAAAGCTGGAAGCAACCAAGGTGTCCGTC +A00126:224:H7HMWDSX3:3:1103:11460:5400 Test_seq 168738 Test_seq 169233 + - UU A00126:224:H7HMWDSX3:3:1103:11460:540097Test_seq16873860151M=169083496AAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTCTATTGGCTAGAATTTTGTTGAGGATTTTTGCATCTATGTTCATGAGGGATATAGGTCTATAATTTTCTTTTTTTGTAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FF::FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:F:FFFFFFFFFFFFFFNM:i:1MD:Z:133A17MC:Z:151MAS:i:146XS:i:87Yt:Z:UU A00126:224:H7HMWDSX3:3:1103:11460:5400145Test_seq16908360151M=168738-496TTCAATCTCTTTTTTTGTTATGGGTCTATTTAGTTGTTCTACTTCTGAATGTGTTAGTTTAGGTAAGTAGTGTTTTTCCAAGAATTCATCCATTTCTTCTAGGTTTTCAAATTTGTTAGAGTACAATTTTTCGTAGTAATCTGAAATGATTFFFFFF::FFFFFFFFFFFFFF:FFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFF,FFF,FFFFF:FFFFFFFFFFFF,FFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:4MD:Z:65G12G39C11A20MC:Z:151MAS:i:131XS:i:76Yt:Z:UU 60 60 AAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTCTATTGGCTAGAATTTTGTTGAGGATTTTTGCATCTATGTTCATGAGGGATATAGGTCTATAATTTTCTTTTTTTGTA TTCAATCTCTTTTTTTGTTATGGGTCTATTTAGTTGTTCTACTTCTGAATGTGTTAGTTTAGGTAAGTAGTGTTTTTCCAAGAATTCATCCATTTCTTCTAGGTTTTCAAATTTGTTAGAGTACAATTTTTCGTAGTAATCTGAAATGATT +A00126:224:H7HMWDSX3:3:1103:4598:6042 Test_seq 394049 Test_seq 394409 + - UU A00126:224:H7HMWDSX3:3:1103:4598:6042161Test_seq3940496018S133M=394259361CTGAATTAGTCCAACTCAATCCATTTTGAGTTAGTTTTTGTGTATGGCGTGAGATATGGACCCTGTTTCATTTTTTTACAAATAGACATCCAGTTGTGCCAGCACCGTTTATTAAAGAGACTGTCTTTTCCCCATTTAATGGACTTCAGCCFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFF:F,FFFFFFFFF:FFFFFFFFFFF,FF:FFFFFFFFNM:i:0MD:Z:133MC:Z:151MAS:i:133XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1103:4598:604281Test_seq39425960151M=394049-361CTGTGGCTGTATCAGTACCAGGCTGTTCTGAGTACCATGGCTGTATAGTAGGTTCTAAGATCAGGTAGTATGAGGCCTCCAACTTTGTTCTTCTTCTGTAATGGCTTACTTTCCATAGGAGTTCAATGGCTGACTTTTTGGAAGGAGATTGFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:18S133MAS:i:151XS:i:0Yt:Z:UU 60 60 CTGAATTAGTCCAACTCAATCCATTTTGAGTTAGTTTTTGTGTATGGCGTGAGATATGGACCCTGTTTCATTTTTTTACAAATAGACATCCAGTTGTGCCAGCACCGTTTATTAAAGAGACTGTCTTTTCCCCATTTAATGGACTTCAGCC CTGTGGCTGTATCAGTACCAGGCTGTTCTGAGTACCATGGCTGTATAGTAGGTTCTAAGATCAGGTAGTATGAGGCCTCCAACTTTGTTCTTCTTCTGTAATGGCTTACTTTCCATAGGAGTTCAATGGCTGACTTTTTGGAAGGAGATTG +A00126:224:H7HMWDSX3:3:1103:21197:7075 Test_seq 166043 Test_seq 166468 + - UU A00126:224:H7HMWDSX3:3:1103:21197:707597Test_seq16604360151M=166318426ATGAGAATTTGGGGTCTGTATCCCATTGGTCTCCTGCTCCCTCAGGAGTTGTCTGTTGTGCTCCCTGACAGGGCAGACAGCGATTGTGGCCGGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF,F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFFF,F,FFFFFFF:F,NM:i:1MD:Z:79T71MC:Z:151MAS:i:146XS:i:36Yt:Z:UU A00126:224:H7HMWDSX3:3:1103:21197:7075145Test_seq16631860151M=166043-426CCACAATTCAAAGTGGGATGCAGAGTGTTTCCATAATAGAATTATTTTGCCCATTGACTTAGAAATCCCCTCAAACCAAGTTCCCCAGACCCCAGCCCCTGCTCCGCTGACCTTTGAAGCTTTCATTTTATCCCGGAAACCTCTTTGCTTTFFFFFFFFFFF:FFFF,FF:FFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFF,FF:FFFF:FFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFF:F:FFFFFFF::FFFFFFFFNM:i:6MD:Z:24A5T21G11G2T10T72MC:Z:151MAS:i:121XS:i:41Yt:Z:UU 60 60 ATGAGAATTTGGGGTCTGTATCCCATTGGTCTCCTGCTCCCTCAGGAGTTGTCTGTTGTGCTCCCTGACAGGGCAGACAGCGATTGTGGCCGGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTT CCACAATTCAAAGTGGGATGCAGAGTGTTTCCATAATAGAATTATTTTGCCCATTGACTTAGAAATCCCCTCAAACCAAGTTCCCCAGACCCCAGCCCCTGCTCCGCTGACCTTTGAAGCTTTCATTTTATCCCGGAAACCTCTTTGCTTT +A00126:224:H7HMWDSX3:3:1103:11695:7529 Test_seq 167155 Test_seq 167618 + - UU A00126:224:H7HMWDSX3:3:1103:11695:7529161Test_seq16715545151M=167468464ATTTCTCTAATGGCTAATGATCGGGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAATCATGTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFF:FFF:FF:FFFF:FFFFFFF:F:FFFF,FFFNM:i:2MD:Z:23A64T62MC:Z:151MAS:i:141XS:i:119XA:Z:Test_seq,-213729,151M,7;Yt:Z:UU A00126:224:H7HMWDSX3:3:1103:11695:752981Test_seq16746852151M=167155-464TGTTTTGTATTCTGTTTATGCCCTGTATTAGGGCTCCTAGGGTTGATCCTATTTTTTCTTCCATGATCTTTATCATTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:74G76MC:Z:151MAS:i:146XS:i:121XA:Z:Test_seq,-400931,151M,6;Yt:Z:UU 45 52 ATTTCTCTAATGGCTAATGATCGGGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAATCATGT TGTTTTGTATTCTGTTTATGCCCTGTATTAGGGCTCCTAGGGTTGATCCTATTTTTTCTTCCATGATCTTTATCATTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTT +A00126:224:H7HMWDSX3:3:1103:6180:8249 Test_seq 167960 Test_seq 212798 + + UU A00126:224:H7HMWDSX3:3:1103:6180:8249129Test_seq16796060151M=21279844839TTGGTATTTGGATTGGAAGTGCGTTATATGTATAAATGGCTTTTGGTAGAATAGACATTTTTACTATGTTAAGTCTTCCTATCCATGAGCAGGGTATGTTCTTCCACTTAAGTATGTCCTTTTGAATTTCTTGTAGCAGAGTTTTATAGTTF,:FFFFFFFFFFFFFF:FFFFFFFFFF,FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFFFFF:FFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFF:FFFFFF:FNM:i:1MD:Z:34G116MC:Z:14S132M5SAS:i:146XS:i:46Yt:Z:UU A00126:224:H7HMWDSX3:3:1103:6180:824965Test_seq2127986014S132M5S=167960-44839AAGAAACCCTAAGAATTCCTCTACATCAACAAAAAGAACATCGAAGAGGAAATAACGAAATCAATACCATTCACAGTAGCCCCCAAGAAGATAAAATACTTAGGAATAAATCTTACCAAGGATGTAAAAGACCTATACAAAGAAAACTATAFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF,FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFF:FFFFFFFFFF:FFFFFF:FFFFFFFFFFFF::FFFFFFFFFFFFFFFFNM:i:3MD:Z:28A13C62A26MC:Z:151MAS:i:117XS:i:85Yt:Z:UU 60 60 TTGGTATTTGGATTGGAAGTGCGTTATATGTATAAATGGCTTTTGGTAGAATAGACATTTTTACTATGTTAAGTCTTCCTATCCATGAGCAGGGTATGTTCTTCCACTTAAGTATGTCCTTTTGAATTTCTTGTAGCAGAGTTTTATAGTT AAGAAACCCTAAGAATTCCTCTACATCAACAAAAAGAACATCGAAGAGGAAATAACGAAATCAATACCATTCACAGTAGCCCCCAAGAAGATAAAATACTTAGGAATAAATCTTACCAAGGATGTAAAAGACCTATACAAAGAAAACTATA +A00126:224:H7HMWDSX3:3:1103:18521:9799 ! 0 Test_seq 167943 - + MU A00126:224:H7HMWDSX3:3:1103:18521:979981Test_seq1681630151M=167943-371ATCTTCTTGGGGGCTACTGTGAATGGTATTGATTTGGTTATTTCCTCTTCGGTATTCTTTTTGTTGATGTAGAGGAATCCAAGTGATTTTTGTATGTTTATTTTATATCCTGAGACTCTGCCAAACTCTTCTATTAGTTTCAGTAGTTTTCF:FFFFFFFFFFF,FFFFFFFFFFFFFFFFFF:FFFF,FFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFNM:i:5MD:Z:6C46G53A0T10T31MC:Z:151MAS:i:126XS:i:126XA:Z:Test_seq,+212725,151M,5;Yt:Z:MU A00126:224:H7HMWDSX3:3:1103:18521:9799161Test_seq16794360151M=168163371CCCCTTAAAATATGACATTGGTATTTGGATTGGAAGTGCGTTATATGTATAGATGGCTTTTGGTAGAATAGACATTTTTACTATGTTAAGTCTTCCTATCCATGAGCAGGGTATGTTTTTCCACTTAAGTATGTCCTTTTGAATTTCTTGTFFF::F:FF:FFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFF:FFFFFFFF:NM:i:1MD:Z:117C33MC:Z:151MAS:i:146XS:i:78Yt:Z:MU 0 60 ATCTTCTTGGGGGCTACTGTGAATGGTATTGATTTGGTTATTTCCTCTTCGGTATTCTTTTTGTTGATGTAGAGGAATCCAAGTGATTTTTGTATGTTTATTTTATATCCTGAGACTCTGCCAAACTCTTCTATTAGTTTCAGTAGTTTTC CCCCTTAAAATATGACATTGGTATTTGGATTGGAAGTGCGTTATATGTATAGATGGCTTTTGGTAGAATAGACATTTTTACTATGTTAAGTCTTCCTATCCATGAGCAGGGTATGTTTTTCCACTTAAGTATGTCCTTTTGAATTTCTTGT +A00126:224:H7HMWDSX3:3:1103:18638:10066 ! 0 Test_seq 167943 - + MU A00126:224:H7HMWDSX3:3:1103:18638:1006681Test_seq1681630151M=167943-371ATCTTCTTGGGGGCTACTGTGAATGGTATTGATTTGGTTATTTCCTCTTCGGTATTCTTTTTGTTGATGTAGAGGAATCCAAGTGATTTTTGTATGTTTATTTTATATCCTGAGACTCTGCCAAACTCTTCTATTAGTTTCAGTAGTTTTCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FNM:i:5MD:Z:6C46G53A0T10T31MC:Z:151MAS:i:126XS:i:126XA:Z:Test_seq,+212725,151M,5;Yt:Z:MU A00126:224:H7HMWDSX3:3:1103:18638:10066161Test_seq16794360151M=168163371CCCCTTAAAATATGACATTGGTATTTGGATTGGAAGTGCGTTATATGTATAGATGGCTTTTGGTAGAATAGACATTTTTACTATGTTAAGTCTTCCTATCCATGAGCAGGGTATGTTTTTCCACTTAAGTATGTCCTTTTGAATTTCTTGTFFFFFFFF:,FFFF:,:FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFNM:i:1MD:Z:117C33MC:Z:151MAS:i:146XS:i:78Yt:Z:MU 0 60 ATCTTCTTGGGGGCTACTGTGAATGGTATTGATTTGGTTATTTCCTCTTCGGTATTCTTTTTGTTGATGTAGAGGAATCCAAGTGATTTTTGTATGTTTATTTTATATCCTGAGACTCTGCCAAACTCTTCTATTAGTTTCAGTAGTTTTC CCCCTTAAAATATGACATTGGTATTTGGATTGGAAGTGCGTTATATGTATAGATGGCTTTTGGTAGAATAGACATTTTTACTATGTTAAGTCTTCCTATCCATGAGCAGGGTATGTTTTTCCACTTAAGTATGTCCTTTTGAATTTCTTGT +A00126:224:H7HMWDSX3:3:1103:30282:10160 Test_seq 341142 Test_seq 351462 + - UR A00126:224:H7HMWDSX3:3:1103:30282:10160161Test_seq3411426030M121S=35131210321GCGGGGGCCTGAACCCTGCTCTGCACGATTGATCATACCTAAAATATTGGCAGTAATTACACTATCATCTATTTACAAATCATTGTTCATAGTTTCCAGTTGTCTCATAATTTTTTTTTTAATAGTTGAGTTGCTCAAAACAAGACCTAAAFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFF,FF:FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFNM:i:0MD:Z:30MC:Z:151MAS:i:30XS:i:0SA:Z:Test_seq,351127,+,29S122M,60,0;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1103:30282:101602209Test_seq3511276029H122M=351312336TGATCATACCTAAAATATTGGCAGTAATTACACTATCATCTATTTACAAATCATTGTTCATAGTTTCCAGTTGTCTCATAATTTTTTTTTTAATAGTTGAGTTGCTCAAAACAAGACCTAAAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFF,FF:FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFNM:i:0MD:Z:122MC:Z:151MAS:i:122XS:i:0SA:Z:Test_seq,341142,+,30M121S,60,0;Yt:Z:UR A00126:224:H7HMWDSX3:3:1103:30282:1016081Test_seq35131260151M=341142-10321TCTCTATCCTTCTTGTTTCTTTTTCCTTACCATTTGATGGTTGAAGAAACTGATCATTTTTTCTGTAGAGCTTCCCATATTCTAGGTTTAGGCGTCTCAGTGGTGTTGTGTAACACATTCTTCTGTCCCTTTATTTTCTGTAATTGGCTACFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:30M121SAS:i:151XS:i:0Yt:Z:UR 60 60 GCGGGGGCCTGAACCCTGCTCTGCACGATTGATCATACCTAAAATATTGGCAGTAATTACACTATCATCTATTTACAAATCATTGTTCATAGTTTCCAGTTGTCTCATAATTTTTTTTTTAATAGTTGAGTTGCTCAAAACAAGACCTAAA TCTCTATCCTTCTTGTTTCTTTTTCCTTACCATTTGATGGTTGAAGAAACTGATCATTTTTTCTGTAGAGCTTCCCATATTCTAGGTTTAGGCGTCTCAGTGGTGTTGTGTAACACATTCTTCTGTCCCTTTATTTTCTGTAATTGGCTAC +A00126:224:H7HMWDSX3:3:1103:28402:11537 Test_seq 168347 Test_seq 168776 + - UU A00126:224:H7HMWDSX3:3:1103:28402:11537161Test_seq1683476018S133M=168626430ATTTGGATATTAATTAGATCATGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCTTACCAATCTGGATACCCTTTATTTCTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGCACGATGTTGAATAAGAGCGGTGATAAAGGGCAF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF,,FFFFF:FFFFF:F,F,FFFFFFFFFFFFFFF,:FF,FFFF:FFFFFFFFFFFFFF,FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF,FFFNM:i:3MD:Z:18A21G59T32MC:Z:151MAS:i:118XS:i:71Yt:Z:UU A00126:224:H7HMWDSX3:3:1103:28402:1153781Test_seq16862660151M=168347-430ATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCFFFFFFFFF:FFFFFFFF:FFFFF,F:FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:90T60MC:Z:18S133MAS:i:146XS:i:72Yt:Z:UU 60 60 ATTTGGATATTAATTAGATCATGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCTTACCAATCTGGATACCCTTTATTTCTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGCACGATGTTGAATAAGAGCGGTGATAAAGGGCA ATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATC +A00126:224:H7HMWDSX3:3:1103:10149:12461 Test_seq 167571 Test_seq 168026 + - UU A00126:224:H7HMWDSX3:3:1103:10149:1246197Test_seq16757127151M=167876456CCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATCACTTCCCCAATTGACTGACACTGGTCCTTTGTCAAATATCAGCTGCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFNM:i:1MD:Z:106T44MC:Z:151MAS:i:146XS:i:133XA:Z:Test_seq,-213317,143M2I6M,4;Yt:Z:UU A00126:224:H7HMWDSX3:3:1103:10149:12461145Test_seq16787660151M=167571-456TCAGTAATGCTTTGCTTATCCGGAGGTTCTTTCCCTTCCATATGAAATTAGTGATTTATTTCTCTATCCCCTTAAAATATGACATTGGTATTTGGATTGGAAGTGCGTTATATGTATAGATGGCTTTTGGTAGAATAGACATTTTTACTAT:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFF:FFFFFFF:FFFFFFFF,F,F:FF,FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFNM:i:2MD:Z:23G33G93MC:Z:151MAS:i:141XS:i:46Yt:Z:UU 27 60 CCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATCACTTCCCCAATTGACTGACACTGGTCCTTTGTCAAATATCAGCTGC TCAGTAATGCTTTGCTTATCCGGAGGTTCTTTCCCTTCCATATGAAATTAGTGATTTATTTCTCTATCCCCTTAAAATATGACATTGGTATTTGGATTGGAAGTGCGTTATATGTATAGATGGCTTTTGGTAGAATAGACATTTTTACTAT +A00126:224:H7HMWDSX3:3:1103:29125:12633 Test_seq 168241 Test_seq 168582 + - UU A00126:224:H7HMWDSX3:3:1103:29125:12633161Test_seq16824136103M4I42M2S=168432342CCAAGTGATTTTTGTATGTTTATTTTATAACCTGAGACTCTGCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGGTTTTCCATGTATACGATCGATCATGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCTAAFFFFFFFF,FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFF:F:F:FFFFFFFFFFF,:F,FFFFFFFFFFFF,F:,F,FFFFFFFF,FF:,FFFFF:FFFFFFFFF:F:F,FFFFFFFFFF:FFFF,FFFFFFFF,,FFF:,FNM:i:8MD:Z:30T10T63G18A20MC:Z:151MAS:i:115XS:i:95XA:Z:Test_seq,-212653,2S37M4I108M,12;Yt:Z:UU A00126:224:H7HMWDSX3:3:1103:29125:1263381Test_seq16843260151M=168241-342GGCTAGGACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGFFF:F:F,,FFFFF:FFFFFFFFFFFFFFFFFF:FFFFFFF:F:FFFFF:FFFFFFFFFFFFFF:FF,FFFFF,FF:FFFFFFFFFFF:F:FFF,FFFF:FF:FFFFFFFFFF:FFFFFFFFFFFFFFF:FF:F:FFFFFFFFFFFFFFFFNM:i:1MD:Z:63T87MC:Z:103M4I42M2SAS:i:146XS:i:80Yt:Z:UU 36 60 CCAAGTGATTTTTGTATGTTTATTTTATAACCTGAGACTCTGCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGGTTTTCCATGTATACGATCGATCATGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCTAA GGCTAGGACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGG +A00126:224:H7HMWDSX3:3:1103:17797:13119 ! 0 Test_seq 261168 - - MU A00126:224:H7HMWDSX3:3:1103:17797:1311997Test_seq261103066M=26110366CTTGTGTCCTTGGTGTTCTTCTTTCTCCTTTGGTCCAGGTGAGTTGAGACCAATTGATGCATCTTAFFF:FFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFNM:i:4MD:Z:21A10A8G11C12MC:Z:66MAS:i:46XS:i:46XA:Z:Test_seq,+399637,66M,4;Test_seq,+150455,66M,5;Test_seq,-130422,57M9S,3;Test_seq,+48182,66M,5;Yt:Z:MU A00126:224:H7HMWDSX3:3:1103:17797:13119145Test_seq2611033166M=261103-66CTTGTGTCCTTGGTGTTCTTCATTCTCCTTTGATCCAGGTGGGTTGAGACCAACTGATGCATCTTAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:66MC:Z:66MAS:i:66XS:i:56XA:Z:Test_seq,-399637,66M,2;Test_seq,-150455,66M,3;Yt:Z:MU 0 31 CTTGTGTCCTTGGTGTTCTTCTTTCTCCTTTGGTCCAGGTGAGTTGAGACCAATTGATGCATCTTA CTTGTGTCCTTGGTGTTCTTCATTCTCCTTTGATCCAGGTGGGTTGAGACCAACTGATGCATCTTA +A00126:224:H7HMWDSX3:3:1103:8296:13730 ! 0 Test_seq 400921 - + MU A00126:224:H7HMWDSX3:3:1103:8296:1373081Test_seq151727059M=400921249137TTCTTTATAATGTTTTGTATACTGTTTATGCCATGTATTAGGGCTCCTAACATTGTCCCFFFFF,:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:2MD:Z:5A42C10MC:Z:59MAS:i:49XS:i:49XA:Z:Test_seq,-49420,59M,2;Test_seq,-19079,59M,4;Test_seq,+213518,52M7S,2;Yt:Z:MU A00126:224:H7HMWDSX3:3:1103:8296:13730161Test_seq4009213159M=151727-249137TTTTTGGTAATGTTTTGTATTCTGTTTATGCCTTGTATTAGGGCTCCTAACGTTGTGCCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:56C2MC:Z:59MAS:i:56XS:i:46XA:Z:Test_seq,-213518,59M,3;Yt:Z:MU 0 31 TTCTTTATAATGTTTTGTATACTGTTTATGCCATGTATTAGGGCTCCTAACATTGTCCC TTTTTGGTAATGTTTTGTATTCTGTTTATGCCTTGTATTAGGGCTCCTAACGTTGTGCC +A00126:224:H7HMWDSX3:3:1103:32651:15358 Test_seq 168430 Test_seq 168845 + - UU A00126:224:H7HMWDSX3:3:1103:32651:15358161Test_seq16843060151M=168695416CTGGCTAAGACTTCCAACACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATCTTGGCTGTTGGCTTTGCATAGATGCCCTGTAGTATGTTGAFFFFF,:::FFFFFFFFFFFF,,F,,F,F:FFFFFFFF,FFF:FFFFF:FFFF,,FFFFFFFFFFFF:FFFFFFFFFFF,FFFF,FFFFF,,F:FFFF:,,:,F:FFF,:,::FFF,F::FFF::F:FF,:FFFFFFF,,,:,,F,:F:FFNM:i:8MD:Z:7G6A1G0T47T44A28T2T8MC:Z:151MAS:i:113XS:i:69Yt:Z:UU A00126:224:H7HMWDSX3:3:1103:32651:1535881Test_seq16869560151M=168430-416TTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTCTATTGGCTAGAATTTTGTTGAGGATTTTTGCATCFFFFFFFFFF:F::FFFFFFFFFF:FFFFFFFFFFFFF:FF:FF,FFFF:FFFFFFF:FFFFFFF:FF::FFFFFF:FFFFFFFFFFFF::FFFFF:FFFF:FFFFFFFF:FFF,FF:FFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFNM:i:1MD:Z:21T129MC:Z:151MAS:i:146XS:i:74Yt:Z:UU 60 60 CTGGCTAAGACTTCCAACACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATCTTGGCTGTTGGCTTTGCATAGATGCCCTGTAGTATGTTGA TTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTCTATTGGCTAGAATTTTGTTGAGGATTTTTGCATC +A00126:224:H7HMWDSX3:3:1103:13693:17065 Test_seq 170079 Test_seq 170414 + - UU A00126:224:H7HMWDSX3:3:1103:13693:17065161Test_seq17007960151M=170264336TTGATTTATGTATCTTGCAGCCCTGTCATTGGGTGCGTAAATATTTAATATGGTTATGTCTTCCTGATCAATTGTCCCTTTTATCATTATATAGTGTCCTTCTTTATCCTTTGTGGTGGATTTAAGTCTAAAATCTATTTTGTCAGAAATTFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFF:FFFFFFFFFFFFFFFFF,FFFFFFFFFFFFF,FFFF:F::FFFFFFFFFFFFFFF,FFFNM:i:5MD:Z:28C28A58C0A14G18MC:Z:151MAS:i:126XS:i:87Yt:Z:UU A00126:224:H7HMWDSX3:3:1103:13693:1706581Test_seq17026460151M=170079-336TTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCTGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFNM:i:1MD:Z:112C38MC:Z:151MAS:i:146XS:i:80Yt:Z:UU 60 60 TTGATTTATGTATCTTGCAGCCCTGTCATTGGGTGCGTAAATATTTAATATGGTTATGTCTTCCTGATCAATTGTCCCTTTTATCATTATATAGTGTCCTTCTTTATCCTTTGTGGTGGATTTAAGTCTAAAATCTATTTTGTCAGAAATT TTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCTGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATT +A00126:224:H7HMWDSX3:3:1103:31503:17127 Test_seq 126887 Test_seq 127296 + - UU A00126:224:H7HMWDSX3:3:1103:31503:1712797Test_seq12688760151M=127146410AGATATTTTTATATCACATTTCAGTTATCACACATATTTTGAAATATCCTTCGCACTTATCACCGAGTCAGAATTTTGGAGCTTAATAGGCCCCCAGACATCCCTGGGTGGTGTAGGCAGTTAAACACTCAGCTGCTAACTGAAAGGTTGGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1103:31503:17127145Test_seq12714660151M=126887-410AGGGTCGCCATGAGTTGGGGCTGGTGCTGTGGCAGCTGTTGTTGTTTGTTTGTTTGTTTTTATTAGACCCACAATGAAATCCTCTTATTTAATATATTTATTATAAATTTGTAATTATTAATAACTATGTCAGTATCATTGGTTTCTTGTG:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU 60 60 AGATATTTTTATATCACATTTCAGTTATCACACATATTTTGAAATATCCTTCGCACTTATCACCGAGTCAGAATTTTGGAGCTTAATAGGCCCCCAGACATCCCTGGGTGGTGTAGGCAGTTAAACACTCAGCTGCTAACTGAAAGGTTGG AGGGTCGCCATGAGTTGGGGCTGGTGCTGTGGCAGCTGTTGTTGTTTGTTTGTTTGTTTTTATTAGACCCACAATGAAATCCTCTTATTTAATATATTTATTATAAATTTGTAATTATTAATAACTATGTCAGTATCATTGGTTTCTTGTG +A00126:224:H7HMWDSX3:3:1103:23981:17065 ! 0 Test_seq 432837 - + NR A00126:224:H7HMWDSX3:3:1103:23981:1706581Test_seq43293160130M21S=432837-224GGAGCTTGCTATTATTTTTTTAATCTTTAACCACAAACTAAGCTTCATTGAAATACCTTTAAAAACTCTTGAGCATATATACCTGTGTGAGTAAACACATCAGTTGTTATTTGTTTCAGATATTCAGATCGATCATCTGAGTGAATTTCCA,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFNM:i:0MD:Z:130MC:Z:151MAS:i:130XS:i:0Yt:Z:NR A00126:224:H7HMWDSX3:3:1103:23981:17065161Test_seq43283760151M=432931224TAACGAGATGTGGAGTAAGGAACTGAGTAGCCTAGAGTGTGAGTTGTTAACCATAGTCCCATTTTGACTAAAGATTTTTATAAGCTGAGAAAAAGGAGCTTGCTATTATTTTTTTAATCTTTAACCACAAACTAAGCTTCATTGAAATACCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:130M21SAS:i:151XS:i:0Yt:Z:NR 0 60 TAACGAGATGTGGAGTAAGGAACTGAGTAGCCTAGAGTGTGAGTTGTTAACCATAGTCCCATTTTGACTAAAGATTTTTATAAGCTGAGAAAAAGGAGCTTGCTATTATTTTTTTAATCTTTAACCACAAACTAAGCTTCATTGAAATACC +A00126:224:H7HMWDSX3:3:1103:24686:17315 Test_seq 169076 Test_seq 169500 + - UU A00126:224:H7HMWDSX3:3:1103:24686:17315161Test_seq169076482S149M=169350425AGTTACCGTTTCAATCTCTTTTTTTGTTATGCGTCTATTTAGTTGTTCTACTTCTGATTGTGTTAGTTTAGGTAGGTCGTGTTCTTCCAGGACTTCATCCATGTCTTCTAGGTTTGCAAATTTCTTAGAGTACAATTTTTCGTAATAATCTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF:FFF:FF:F:,FFFFFFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFF,,FFFFF,FFFF,FFF:F,FF:FFFFFFFFFFFF,FFFFFFFFFFFFFFFNM:i:13MD:Z:29G25A19A5T3G1A2A9T12T7G3C11A4G6MC:Z:151MAS:i:84XS:i:52Yt:Z:UU A00126:224:H7HMWDSX3:3:1103:24686:1731581Test_seq16935060151M=169076-425AATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGATTCTTTTGTTGCTCACTTTCTATTTGTTCAAGT:FFFFFFFFFFFFFFFF:FFFFF,FFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:117G33MC:Z:2S149MAS:i:146XS:i:101Yt:Z:UU 48 60 AGTTACCGTTTCAATCTCTTTTTTTGTTATGCGTCTATTTAGTTGTTCTACTTCTGATTGTGTTAGTTTAGGTAGGTCGTGTTCTTCCAGGACTTCATCCATGTCTTCTAGGTTTGCAAATTTCTTAGAGTACAATTTTTCGTAATAATCT AATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGATTCTTTTGTTGCTCACTTTCTATTTGTTCAAGT +A00126:224:H7HMWDSX3:3:1103:12192:17691 Test_seq 86584 Test_seq 86878 + - UU A00126:224:H7HMWDSX3:3:1103:12192:17691161Test_seq8658460151M=86728295CTGGTATGGTCTTGCGTCCTACATTCAGGCTATCATCTGATGTTGTTTGTTCTCAAACTGAAGGACTTCCTTTAATAATTCTTGTATGTTTGTTTTGGTTTTTATGTATTCCCTTAATTTCTCTTTATCTGGAAATGTCCTAACTTTGCCAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:25Yt:Z:UU A00126:224:H7HMWDSX3:3:1103:12192:1769181Test_seq8672860151M=86584-295TTTGCCATCATATTTAAGCAAGAGTTTTGCAGGATATATTATACTTCTTTGGCAGTTTTTATCTTTCAAGGTTTTATGTATGTCATCCTATCGCCCTCTTGCCTACATGGTTTTTGCCAAGTAATCAGAGCTTAGTCTTATTGTTTCCCCTFFFFFFFFFFF:FFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU 60 60 CTGGTATGGTCTTGCGTCCTACATTCAGGCTATCATCTGATGTTGTTTGTTCTCAAACTGAAGGACTTCCTTTAATAATTCTTGTATGTTTGTTTTGGTTTTTATGTATTCCCTTAATTTCTCTTTATCTGGAAATGTCCTAACTTTGCCA TTTGCCATCATATTTAAGCAAGAGTTTTGCAGGATATATTATACTTCTTTGGCAGTTTTTATCTTTCAAGGTTTTATGTATGTCATCCTATCGCCCTCTTGCCTACATGGTTTTTGCCAAGTAATCAGAGCTTAGTCTTATTGTTTCCCCT +A00126:224:H7HMWDSX3:3:1103:27145:18161 Test_seq 316265 Test_seq 316334 + - UU A00126:224:H7HMWDSX3:3:1103:27145:1816197Test_seq316265970M=31626570AAACATCCTTCTGGTATTCTCTGCTTTCAGCCAGGATCCATCTGACATCAGCAATGATATCCCTGGTTCCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:70MC:Z:70MAS:i:70XS:i:65XA:Z:Test_seq,+15178,70M,1;Test_seq,+333542,70M,1;Test_seq,-365919,70M,1;Yt:Z:UU A00126:224:H7HMWDSX3:3:1103:27145:18161145Test_seq316265770M=316265-70AAACATCCTTCTGGTATTCTCTGCTTTCAACCATGATCCATCTGACATCAGCAATGATATCCCTGGTTCCFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:2MD:Z:29G3G36MC:Z:70MAS:i:60XS:i:55XA:Z:Test_seq,-15178,70M,3;Test_seq,+365919,70M,3;Test_seq,-333542,70M,3;Yt:Z:UU 9 7 AAACATCCTTCTGGTATTCTCTGCTTTCAGCCAGGATCCATCTGACATCAGCAATGATATCCCTGGTTCC AAACATCCTTCTGGTATTCTCTGCTTTCAACCATGATCCATCTGACATCAGCAATGATATCCCTGGTTCC +A00126:224:H7HMWDSX3:3:1103:4661:20400 Test_seq 170269 Test_seq 170534 + - UU A00126:224:H7HMWDSX3:3:1103:4661:20400161Test_seq17026960151M=170394266TGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCGTGACTCTCTGTCTCTTTATTGGTTCATTTAGTCCATTTACATFF:F:FFFFFFFFFFFFFFF,::F,FFFFFFF:FFFF:FFFFFF:F:F:F:FF:FF,FF:F::FF,FF:FFFFFFFFFFFF:FF,FFF,,FFFFFFFFFFFF,F:F,,,FFFF:FFFFFFF,FFF:FFF:F:,FF:::FFFFFF:FFF,FFNM:i:1MD:Z:132G18MC:Z:46M10I95MAS:i:146XS:i:75Yt:Z:UU A00126:224:H7HMWDSX3:3:1103:4661:2040081Test_seq1703946046M10I95M=170269-266TATTGGTGCATTTTGTCCATTTACATTCAGTGTAATTATAGATAAATAATTTTTTTTAAGTTTTTAGTGCTGTCATTTTGATGCATTTTCTTGTGAGTTTTTGGCCATTTCATTTTTCCACATACTTTTTTGTGCTGAGACGTTTTTCTTAFFFFFFFF:FFF:,FFF:FFFF,,FFFF,FFFFFFFFFFF:FF::FF,:FFF:FFFFFFFF:FFF:FF:,:FFFFFFFF:::F,,FF:F:,FFFF,FFF:FF,:::FFFF,FFFFFF::,:FF:,FFFFF:,FFFF,F:F,,FFFFF,FFFNM:i:17MD:Z:13A16C43C5A4T3G6T44MC:Z:151MAS:i:90XS:i:25Yt:Z:UU 60 60 TGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCGTGACTCTCTGTCTCTTTATTGGTTCATTTAGTCCATTTACAT TATTGGTGCATTTTGTCCATTTACATTCAGTGTAATTATAGATAAATAATTTTTTTTAAGTTTTTAGTGCTGTCATTTTGATGCATTTTCTTGTGAGTTTTTGGCCATTTCATTTTTCCACATACTTTTTTGTGCTGAGACGTTTTTCTTA +A00126:224:H7HMWDSX3:3:1103:23619:20572 Test_seq 47127 Test_seq 47517 + - UU A00126:224:H7HMWDSX3:3:1103:23619:20572161Test_seq4712760151M=47367391GATCTTTTTGACATCAGCAATGATATCTTTTGTTATACATCCTCTTCTGACTCTGCCTTGGATTTCTGGCAGTTCTCTGTCAATGTACTGCTGCAAACACTTTTAAATGATCTTCAGCAAAATTTTACTTGTGTGTGATATTAACGATATTF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFNM:i:1MD:Z:50A100MC:Z:151MAS:i:146XS:i:46Yt:Z:UU A00126:224:H7HMWDSX3:3:1103:23619:2057281Test_seq4736760151M=47127-391CTTCCAAATTTTTTGGCATAGATCAGTGAGTGCTTCCAGCACTGCATCTGTTTGTGGAAACATCTCAACTGGTATTCTGTCAATTCCTGGAGCCTTGTTTTTCACCAATGCCTTTAGTGCACATTGGACCTCTTCCTTGAGTCCAAGTTCTF:FFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:42Yt:Z:UU 60 60 GATCTTTTTGACATCAGCAATGATATCTTTTGTTATACATCCTCTTCTGACTCTGCCTTGGATTTCTGGCAGTTCTCTGTCAATGTACTGCTGCAAACACTTTTAAATGATCTTCAGCAAAATTTTACTTGTGTGTGATATTAACGATATT CTTCCAAATTTTTTGGCATAGATCAGTGAGTGCTTCCAGCACTGCATCTGTTTGTGGAAACATCTCAACTGGTATTCTGTCAATTCCTGGAGCCTTGTTTTTCACCAATGCCTTTAGTGCACATTGGACCTCTTCCTTGAGTCCAAGTTCT +A00126:224:H7HMWDSX3:3:1103:18114:21433 Test_seq 167400 Test_seq 167663 + - UU A00126:224:H7HMWDSX3:3:1103:18114:21433161Test_seq16740060151M=167513264AGATGAGCATAGGTGTTTGATTTCTAGGAGCTCCCAGTTATCTGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTATGTTTATGCCCTGTGTTAGGGCTCCTAGGGTTGATCCTATTTTTTCTTCCATGATCTTTATCGTTTTAGTCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFNM:i:3MD:Z:23T55C14A56MC:Z:151MAS:i:136XS:i:106Yt:Z:UU A00126:224:H7HMWDSX3:3:1103:18114:2143381Test_seq16751358151M=167400-264ATCCTATTTTTTCTTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTFFFFFFFFFFFFFFFFFFFFF:F,FFFFFF:FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF,:FFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFF:,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:124XA:Z:Test_seq,+213375,85M2I23M2I37M2S,5;Yt:Z:UU 60 58 AGATGAGCATAGGTGTTTGATTTCTAGGAGCTCCCAGTTATCTGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTATGTTTATGCCCTGTGTTAGGGCTCCTAGGGTTGATCCTATTTTTTCTTCCATGATCTTTATCGTTTTAGTC ATCCTATTTTTTCTTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTT +A00126:224:H7HMWDSX3:3:1103:22417:22654 ! 0 Test_seq 167167 - + MU A00126:224:H7HMWDSX3:3:1103:22417:2265481Test_seq1674030134M17S=167167-370TGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCGGGTTTCTCTTCATCGTTTTTGGTAATGTTTTGTATTCTGTTTATACCTTGTATTAGGGCTCCTAGGGTTGTCCCAATTTTTTCTTCCATGATCTAAATGGTCTGAATTCAAF:FFFFFF:FFF,FFFFFFFFFFFFFFFFFF,:F,,:F::FFFFFFFFFFFFFFFFFFFFF,FF:FFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFNM:i:7MD:Z:39T14A29G2C22A0T2T19MC:Z:151MAS:i:99XS:i:99XA:Z:Test_seq,+213498,17S134M,7;Yt:Z:MU A00126:224:H7HMWDSX3:3:1103:22417:22654161Test_seq16716752151M=167403370GCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAATCATGTAGATTTTAGAGA:FFFFFFFFFFFFFF,FFFFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:,F::,FFF:F,F,FF,FF,FFF,FFF,FF,F::FF:F::FFF,FFFFF:FF,:FF:FF:FFFFFFFFFFFFF,:FFFFFFFF:FFFNM:i:1MD:Z:76T74MC:Z:134M17SAS:i:146XS:i:121XA:Z:Test_seq,-213717,151M,6;Yt:Z:MU 0 52 TGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCGGGTTTCTCTTCATCGTTTTTGGTAATGTTTTGTATTCTGTTTATACCTTGTATTAGGGCTCCTAGGGTTGTCCCAATTTTTTCTTCCATGATCTAAATGGTCTGAATTCAA GCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAATCATGTAGATTTTAGAGA +A00126:224:H7HMWDSX3:3:1103:8368:23093 Test_seq 167067 Test_seq 167564 + - UU A00126:224:H7HMWDSX3:3:1103:8368:23093161Test_seq16706720151M=167414498GCCTCTCCAACATTTATTATTTTGTGTTTTTTGGATTAATGCCAGTCTAGTTGGTGTGAGATGGAATCTCATCGTAGTTTTAATTTGCATTTCTCTAATGGCTAATGATCGAGAGCATTTTCTCATGTATCTGTTAGCTGCTTGAATATCTFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:F,FFFFFFFFF:FFFFFF:FFFFF:FFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFF,FFFFFF:FFFFF:FFFF:FFFFFFFFF,FFFFFFFFFFFFFFFFFF,FNM:i:3MD:Z:77C57G5C9MC:Z:151MAS:i:136XS:i:126XA:Z:Test_seq,-213817,151M,5;Yt:Z:UU A00126:224:H7HMWDSX3:3:1103:8368:2309381Test_seq16741460151M=167067-498GTTTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATGCCCTGTATTAGGGCTCCTAGGGTTGATCCTATTTTTTCTTCCATGATCTTTATCGTTTTAGCCTTTATGTTTAGGTCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:135T15MC:Z:151MAS:i:146XS:i:106Yt:Z:UU 20 60 GCCTCTCCAACATTTATTATTTTGTGTTTTTTGGATTAATGCCAGTCTAGTTGGTGTGAGATGGAATCTCATCGTAGTTTTAATTTGCATTTCTCTAATGGCTAATGATCGAGAGCATTTTCTCATGTATCTGTTAGCTGCTTGAATATCT GTTTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATGCCCTGTATTAGGGCTCCTAGGGTTGATCCTATTTTTTCTTCCATGATCTTTATCGTTTTAGCCTTTATGTTTAGGTC +A00126:224:H7HMWDSX3:3:1103:1615:23484 Test_seq 166033 Test_seq 166276 + - UU A00126:224:H7HMWDSX3:3:1103:1615:2348497Test_seq16603360151M=166126244TGGTCTTTTTATGAGAATTTGGGGTCTGTATCCCATTGGCCTCCTGCTCCCTCAGGAGTTGTCTGTTGTGCTCCCTAGCAGGGCAGACATCGATTGTGGCCAGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGTTCTCTGGTTCAFFFFFFFFFFFFFF:FF:FFF:::FFFFFFF:,:FFFF,F,FF,FF,FFFF,FFFFFFFFFF:FF:F:FF:FFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF,FFFFFFFFFFFFFF,FF:FFFFFFF:NM:i:5MD:Z:39T36G0A23G37G11MC:Z:151MAS:i:126XS:i:61Yt:Z:UU A00126:224:H7HMWDSX3:3:1103:1615:23484145Test_seq16612660151M=166033-244TTGTGGCCAGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCTTAGTTGTCGTGTGGCCTTGGTGTTCTTCCTTTGCCTTTGCTCCAGGTGGGTTGAGACCAATTGATGFFFFFFFF:FFF,::FFFFFFF::FFFF,FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFF,FFFNM:i:1MD:Z:8G142MC:Z:151MAS:i:146XS:i:82Yt:Z:UU 60 60 TGGTCTTTTTATGAGAATTTGGGGTCTGTATCCCATTGGCCTCCTGCTCCCTCAGGAGTTGTCTGTTGTGCTCCCTAGCAGGGCAGACATCGATTGTGGCCAGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGTTCTCTGGTTCA TTGTGGCCAGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCTTAGTTGTCGTGTGGCCTTGGTGTTCTTCCTTTGCCTTTGCTCCAGGTGGGTTGAGACCAATTGATG +A00126:224:H7HMWDSX3:3:1103:17915:23719 Test_seq 169352 Test_seq 169620 + - UU A00126:224:H7HMWDSX3:3:1103:17915:2371997Test_seq16935260151M=169470269TTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTCATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGGTTCTTTTGTTGCTCACTTTCTATTTGTTCAAGTTGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:,FFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFF,FFF,,F,,:F:FFFFFFFFFFFFFFFFFF:FF:F:FFFFFFF:FFF::F:FFFFF,FF:::FFFFFFFFFFFFFFF,FFFF,NM:i:1MD:Z:79A71MC:Z:151MAS:i:146XS:i:96Yt:Z:UU A00126:224:H7HMWDSX3:3:1103:17915:23719145Test_seq16947060151M=169352-269CTTTTGTTGCTCACTTTCTATTTGTTCAAGTTGTCGGGACATTTCTCTGATTTTAGCTCTTTCTTCTTTTTGTATGTGTGCATTTATCGATATAAATTGGCCTCTGAGCACCGCTTTTGCTGTGTCCCAGAGGTTTTGATAGGAAGTATTTFFFFFF:::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:6MD:Z:35A5G7C0C3G56T39MC:Z:151MAS:i:121XS:i:69Yt:Z:UU 60 60 TTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTCATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGGTTCTTTTGTTGCTCACTTTCTATTTGTTCAAGTTG CTTTTGTTGCTCACTTTCTATTTGTTCAAGTTGTCGGGACATTTCTCTGATTTTAGCTCTTTCTTCTTTTTGTATGTGTGCATTTATCGATATAAATTGGCCTCTGAGCACCGCTTTTGCTGTGTCCCAGAGGTTTTGATAGGAAGTATTT +A00126:224:H7HMWDSX3:3:1103:25238:24126 ! 0 Test_seq 169142 - - NR A00126:224:H7HMWDSX3:3:1103:25238:2412697Test_seq1688086027S124M=168992335TGTGCAAGGGCTCTGATGACTAAGACTATTCTATTGGCTAGAATTTTGTTGAGGATTTTTGCATCTATGTTCATGAGGGATATAGGTCTAAAATTTTCTTTTTTTGCAATGTCTTTACCTGGTTTTGGTATCAGGGAGATGGTAGCTTCATFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFF:FFFFFF:FFFFFFFFFFF:FFFFF:FFFFFFFFF:FFFFFF,FFFFFF:FFNM:i:3MD:Z:79T26A9G7MC:Z:151MAS:i:109XS:i:75Yt:Z:NR A00126:224:H7HMWDSX3:3:1103:25238:24126145Test_seq16899260151M=168808-335GTGTTAAGTCTTCTCTGAAGGTTTGGTAGAACTCTGCAGTGAAGCCGTCTGGGCCAGGACTTTTTTTTGTTGGAAGTTTTTTGATTACCGTTTCAATCTCTTTTTTTGTTATGGGTCTATTTAGTTGTTCTACTTCCGAATGTGTTAGTTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:136T14MC:Z:27S124MAS:i:146XS:i:0Yt:Z:NR 0 60 GTGTTAAGTCTTCTCTGAAGGTTTGGTAGAACTCTGCAGTGAAGCCGTCTGGGCCAGGACTTTTTTTTGTTGGAAGTTTTTTGATTACCGTTTCAATCTCTTTTTTTGTTATGGGTCTATTTAGTTGTTCTACTTCCGAATGTGTTAGTTT +A00126:224:H7HMWDSX3:3:1103:31069:25551 Test_seq 185527 Test_seq 185905 + - UU A00126:224:H7HMWDSX3:3:1103:31069:25551161Test_seq18552760151M=185755379GATCAGTCCAATGTGTGCCAAACCACCAATAATCATAACATAGCCAAGCTGACACAAAATATTAACATCACACACAGAGCAATTCAAGAACTACATCCATAAAACCAAGAAAACTGTATAAAAAAGAGCAACCTGTGAATAAAAAATTGAT,:FFFF,FFFFFF,:,:FFFFFFFFFFFF,FF,F::FF:FFFFFFFFFFFFFFFF,FFFFFF,FFFFFFFFFFFFFFFF,FFF,FFF:FFFFFFFFFFFFFFFFFFFF:FFFFFF,FFFF:FFFFFF,:FF:FF,F,F,FFFFFFFF,:,:NM:i:1MD:Z:149T1MC:Z:151MAS:i:149XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1103:31069:2555181Test_seq18575560151M=185527-379AGAGCAAAATGACATGGGTGGAAAATATGAGGGAAAGAATAGCTTAATACTACTACTAATAAAAATCAGAACATCTAACATATTCTGCAGGCTCTTAGTATGTGCCAGGTGCTGTTTTATGCTTTCATCTGCACTACTTCAGTCAGTCCTC,FF:F:F,::FF,,F:F:F::FFF,FFFFF,FF,,,,FF,,,:F,FF:F,FF:,FFF,FFF,,:FF:FFF::F,:F,F,,,F:F,FFF,,F,,FFF,FFFFF,::,FF:FF:F:FFFF:F:,FFFF:F,FF:FF::,:F,,:F:,FF,,F,NM:i:3MD:Z:44A28G18A58MC:Z:151MAS:i:136XS:i:0Yt:Z:UU 60 60 GATCAGTCCAATGTGTGCCAAACCACCAATAATCATAACATAGCCAAGCTGACACAAAATATTAACATCACACACAGAGCAATTCAAGAACTACATCCATAAAACCAAGAAAACTGTATAAAAAAGAGCAACCTGTGAATAAAAAATTGAT AGAGCAAAATGACATGGGTGGAAAATATGAGGGAAAGAATAGCTTAATACTACTACTAATAAAAATCAGAACATCTAACATATTCTGCAGGCTCTTAGTATGTGCCAGGTGCTGTTTTATGCTTTCATCTGCACTACTTCAGTCAGTCCTC +A00126:224:H7HMWDSX3:3:1103:31901:25989 ! 0 Test_seq 168499 - + NR A00126:224:H7HMWDSX3:3:1103:31901:2598981Test_seq16866560114M37S=168499-280TTGATAAGATCATGTGGTTTTTGTCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGTCTCAGATTATTCTTAAGGTTTCTTCTTTTTTTTGTG,FFF:FFF,FFF,FFFFFFFF:FFFFFFFFFFFFFF:FFFFFF:FFF:FFFFFF,FFFFFFFF:FF:F:FFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFNM:i:2MD:Z:22A28T62MC:Z:151MAS:i:104XS:i:58Yt:Z:NR A00126:224:H7HMWDSX3:3:1103:31901:25989161Test_seq16849960151M=168665280CTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGGACTTTGTCAAATGFFFFFFFFFFFFFFF,FF:FFFFFFFFFFFF,:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F,F,,F:FFFFFFFF:FF:FFFFFFFFFFFFF,F,F:FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:137A13MC:Z:114M37SAS:i:146XS:i:91Yt:Z:NR 0 60 CTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGGACTTTGTCAAATG +A00126:224:H7HMWDSX3:3:1103:15112:26819 Test_seq 170019 Test_seq 170439 + - UU A00126:224:H7HMWDSX3:3:1103:15112:2681997Test_seq17001929151M=170289421GTTGAAGTCTCCTACTATAATTGTGGAGGTGTCTATCTCACTTTTCAGTTCTGATAAAATTTGATTCATGTATCTTGCAGCCCTGTCATTGGGTGCATAAATATTTAATATGGTTATGTCTTCCTGATCAATTGTCCCTTTTATCATTATGFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF::FFFFFFFFFFFFFF:FFFFFFFFFFFFFFF,FFFFFFFF:F:F:F:FFFFFFFFFFFF:FFFFFF:FFFF,F,FFFFFFFF,:FFFFFF,F:FFNM:i:11MD:Z:9C10A7A10G7A5T12T21C7G20A32A0MC:Z:151MAS:i:100XS:i:82XA:Z:Test_seq,-38563,147M4S,13;Yt:Z:UU A00126:224:H7HMWDSX3:3:1103:15112:26819145Test_seq17028960151M=170019-421TTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCTTAATTATAGATAAAFF:FFFFFFFFFF,F:F,FFFF:FFFFFFFFFFFFF:FF::FFFF,::,FF:FF:FFFF:FFFFFFF:F:F::FFFFF:FFFFFF:FFF:FFF:FF::FFFFFFF:F:::FFFFFFF,FF:FFFF,:FFFFFFF:F:FFFFFF:,FFFFFFNM:i:1MD:Z:136G14MC:Z:151MAS:i:146XS:i:84Yt:Z:UU 29 60 GTTGAAGTCTCCTACTATAATTGTGGAGGTGTCTATCTCACTTTTCAGTTCTGATAAAATTTGATTCATGTATCTTGCAGCCCTGTCATTGGGTGCATAAATATTTAATATGGTTATGTCTTCCTGATCAATTGTCCCTTTTATCATTATG TTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCTTAATTATAGATAAA +A00126:224:H7HMWDSX3:3:1103:6307:27007 ! 0 Test_seq 167150 - + NR A00126:224:H7HMWDSX3:3:1103:6307:2700781Test_seq16741622120M31S=167150-386TTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATGCCCTGTATTAGGGCTCCTAGGGTTGTCCCTATTTTTTCTTCCATGATCAGTCATACAGTAATTCTGTGTTTCACTTTTGFFFFFFF:F:FFFFFFFFFFFF,F:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:2MD:Z:97A0T21MC:Z:151MAS:i:110XS:i:100XA:Z:Test_seq,+213499,31S120M,4;Yt:Z:NR A00126:224:H7HMWDSX3:3:1103:6307:27007161Test_seq16715042151M=167416386TTTGCATTTCTCTAATGGCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAATFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::,FFFFFFF,FFF::F:FFFFF:FFFFF,FFFFFFFF:F,NM:i:1MD:Z:93T57MC:Z:120M31SAS:i:146XS:i:126XA:Z:Test_seq,-213734,151M,5;Yt:Z:NR 0 42 TTTGCATTTCTCTAATGGCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAAT +A00126:224:H7HMWDSX3:3:1103:27597:28244 Test_seq 260971 Test_seq 261044 + - UU A00126:224:H7HMWDSX3:3:1103:27597:28244161Test_seq2609714274M=26097174CAGGGGTTCTCTGTTGTGTTCCCTGTCAGGGCAGTCATCGGTTGTAGCCGGGCACCATCTAGTTCTTCTGGTCTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::NM:i:1MD:Z:48T25MC:Z:74MAS:i:69XS:i:54Yt:Z:UU A00126:224:H7HMWDSX3:3:1103:27597:2824481Test_seq260971774M=260971-74CAGGGGTTCTCTTTTGTGTTCCCTGTCTGGGCAGTCATCTGTTGTAGCCGGGCACCATCTAGTTCTTCTGGTCTFFFFF,FFFFFF,FFFFFFFFFFFFFF,FFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:4MD:Z:12G14A11G8T25MC:Z:74MAS:i:54XS:i:49XA:Z:Test_seq,-48050,74M,5;Test_seq,-150323,74M,5;Yt:Z:UU 42 7 CAGGGGTTCTCTGTTGTGTTCCCTGTCAGGGCAGTCATCGGTTGTAGCCGGGCACCATCTAGTTCTTCTGGTCT CAGGGGTTCTCTTTTGTGTTCCCTGTCTGGGCAGTCATCTGTTGTAGCCGGGCACCATCTAGTTCTTCTGGTCT +A00126:224:H7HMWDSX3:3:1103:4191:28541 Test_seq 40015 Test_seq 168498 + + UU A00126:224:H7HMWDSX3:3:1103:4191:2854165Test_seq40015184S147M=168498128484CATGAATAAAACAAAAGACAAGAATCACATGATTTTATCAATTGATGCAGAAAAGGCATTTGACAAAGTTCAACACTCATTCATGATAAAAACCCTCAGCAAAATAGGAATAGAAGGAAAATTCCTCAACATAATAAAGGGCACTTATACAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:6MD:Z:25A23G22C16T10C38T7MC:Z:151MAS:i:117XS:i:107XA:Z:Test_seq,-152855,151M,9;Yt:Z:UU A00126:224:H7HMWDSX3:3:1103:4191:28541129Test_seq16849860151M=40015-128484TCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGGACTTTGTCAAATFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFNM:i:1MD:Z:138A12MC:Z:4S147MAS:i:146XS:i:91Yt:Z:UU 18 60 CATGAATAAAACAAAAGACAAGAATCACATGATTTTATCAATTGATGCAGAAAAGGCATTTGACAAAGTTCAACACTCATTCATGATAAAAACCCTCAGCAAAATAGGAATAGAAGGAAAATTCCTCAACATAATAAAGGGCACTTATACA TCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGGACTTTGTCAAAT +A00126:224:H7HMWDSX3:3:1103:11668:28620 ! 0 Test_seq 169461 - - NR A00126:224:H7HMWDSX3:3:1103:11668:28620161Test_seq1691626026S125M=169311300TCGTCAAAAAGAACGTTTCAAGATCTAAGAATTTATCCATTTCTTCTAGGTTTTCAAATTTGTTAGAGTACAATTTTACGTAGTAATCTGAAGTGATTCTTTTAATTTCATTTGGCTCTGTTGTGATGTGGTCCTTCTCATTTCTTATTCGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFF:FFFFFFFFFFFFFFFFF::FFFFF:FF:FNM:i:5MD:Z:7C31C26A39T6G11MC:Z:151MAS:i:100XS:i:61Yt:Z:NR A00126:224:H7HMWDSX3:3:1103:11668:2862081Test_seq16931160151M=169162-300TTCTTTAGTCAGTCTAGCTAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:18C132MC:Z:26S125MAS:i:146XS:i:107Yt:Z:NR 0 60 TTCTTTAGTCAGTCTAGCTAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCT +A00126:224:H7HMWDSX3:3:1103:13485:30545 Test_seq 168615 Test_seq 212799 - - UU A00126:224:H7HMWDSX3:3:1103:13485:30545177Test_seq16846560151M=21264944185CGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:30T120MC:Z:151MAS:i:146XS:i:91Yt:Z:UU A00126:224:H7HMWDSX3:3:1103:13485:30545113Test_seq21264927151M=168465-44185GGCAAGGAAGAAGTAAAGTTATCACTATTTGCAGATGACATGATTATATACACAGAAAACCCTAAGGAATCCTCCAGAAAACTACTGAAACTAATAGAAGAGTTTGGCAGAGTCTCAGGTTATAAAATAAACATACAAAAATCACTTGGATFF:FF,FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFNM:i:7MD:Z:8G8A5T20C0T17T61G25MC:Z:151MAS:i:116XS:i:101XA:Z:Test_seq,+168239,151M,10;Yt:Z:UU 60 27 CGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTT GGCAAGGAAGAAGTAAAGTTATCACTATTTGCAGATGACATGATTATATACACAGAAAACCCTAAGGAATCCTCCAGAAAACTACTGAAACTAATAGAAGAGTTTGGCAGAGTCTCAGGTTATAAAATAAACATACAAAAATCACTTGGAT +A00126:224:H7HMWDSX3:3:1103:24207:31986 ! 0 Test_seq 227720 - + NR A00126:224:H7HMWDSX3:3:1103:24207:31986145Test_seq22793360118M33S=227720-331GTGGAGGTACAGTGTGGTGCTTCTGTTTATAGCACCATGTTGCCTCTGTCCCTGACTGTACATTTTTGTAATGTCCACTCAATTCATGTGCATGGAATTTGATTTATTCTGGAGGTTAGATCACCGACCCTGGAAGCAGCAGTCAAGGAATFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:118MC:Z:151MAS:i:118XS:i:0Yt:Z:NR A00126:224:H7HMWDSX3:3:1103:24207:3198697Test_seq22772060151M=227933331TGTTTCCTGTCTGTTGATCTCTGACAGGAGTTCAAGACGGCACATCAGTGCTGCATTAGCTGACGGGGGACCTCTGCTCATATCTTTCCTCACTCTCTGTTCTCAGTTTCTTATTCCTCAGTGCTTGATTGAATTCTTTATCTCTTCATTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF,F:FFFFF,FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:F,FFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:118M33SAS:i:151XS:i:0Yt:Z:NR 0 60 TGTTTCCTGTCTGTTGATCTCTGACAGGAGTTCAAGACGGCACATCAGTGCTGCATTAGCTGACGGGGGACCTCTGCTCATATCTTTCCTCACTCTCTGTTCTCAGTTTCTTATTCCTCAGTGCTTGATTGAATTCTTTATCTCTTCATTT +A00126:224:H7HMWDSX3:3:1103:24966:32299 Test_seq 170889 Test_seq 171379 + - UU A00126:224:H7HMWDSX3:3:1103:24966:32299161Test_seq17088960151M=171229491CGTTCTGTTTTGTGACCTTGTATTGTGCTGGTACCTGATATTGTTGGTCATCAGGCCGAACAATCTCCTTTAGCATTTCTTGCAGTCTTGGTTTAGTTTTTGCAAATTCTCTAAACTTGTGTTTATCTGTAAATATCTTAATTTCTCCTTCFF:FFFFFFFFF,FFFFFFFFFFFFFFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFF,F:FF::FF,:FFFFFFFFFFFFFFFFFFFFF,:,:FFFFFFFFFFFFFFF:FFF:FFFF,F:,NM:i:3MD:Z:42A14A12A80MC:Z:151MAS:i:136XS:i:23Yt:Z:UU A00126:224:H7HMWDSX3:3:1103:24966:3229981Test_seq17122960151M=170889-491TTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATGGGGTTCGATGAGCATCTTGGATAGATATCCTTTCGTCTTTCATGATGTCAGGGAAGTTTTGTGTCAGGATTTCTTCAACTFFFFF:FFF:FFFFF:FFF,FFFFFFFFFFF:F,FFFF:FFF:FFFFFFF:F:FFFFFFFF:FFFFF:FFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF,F:FFFFFFFFFFFFF,FFFFFFFFFFF:FFFNM:i:1MD:Z:140G10MC:Z:151MAS:i:146XS:i:72Yt:Z:UU 60 60 CGTTCTGTTTTGTGACCTTGTATTGTGCTGGTACCTGATATTGTTGGTCATCAGGCCGAACAATCTCCTTTAGCATTTCTTGCAGTCTTGGTTTAGTTTTTGCAAATTCTCTAAACTTGTGTTTATCTGTAAATATCTTAATTTCTCCTTC TTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATGGGGTTCGATGAGCATCTTGGATAGATATCCTTTCGTCTTTCATGATGTCAGGGAAGTTTTGTGTCAGGATTTCTTCAACT +A00126:224:H7HMWDSX3:3:1103:24894:32424 ! 0 Test_seq 227720 - + NR A00126:224:H7HMWDSX3:3:1103:24894:32424145Test_seq22793360118M33S=227720-331GTGGAGGTACAGTGTGGTGCTTCTGTTTATAGCACCATGTTGCCTCTGTCCCTGACTGTACATTTTTGTAATGTCCACTCAATTCATGTGCATGGAATTTGATTTATTCTGGAGGTTAGATCACCGACCCTGGAAGCAGCAGTCAAGGAATFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:118MC:Z:151MAS:i:118XS:i:0Yt:Z:NR A00126:224:H7HMWDSX3:3:1103:24894:3242497Test_seq22772060151M=227933331TGTTTCCTGTCTGTTGATCTCTGACAGGAGTTCAAGACGGCACATCAGTGCTGCATTAGCTGACGGGGGACCTCTGCTCATATCTTTCCTCACTCTCTGTTCTCAGTTTCTTATTCCTCAGTGCTTGATTGAATTCTTTATCTCTTCATTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:118M33SAS:i:151XS:i:0Yt:Z:NR 0 60 TGTTTCCTGTCTGTTGATCTCTGACAGGAGTTCAAGACGGCACATCAGTGCTGCATTAGCTGACGGGGGACCTCTGCTCATATCTTTCCTCACTCTCTGTTCTCAGTTTCTTATTCCTCAGTGCTTGATTGAATTCTTTATCTCTTCATTT +A00126:224:H7HMWDSX3:3:1103:14299:32863 Test_seq 170143 Test_seq 170496 + - UU A00126:224:H7HMWDSX3:3:1103:14299:32863161Test_seq1701436013S138M=170346354GAACAGTGATGAATGATCAATTGTCCCTTTTATCATTATATAGTGTCCTTCTTTATCCTTTGTGGCGGATTTAAGTCTAAAGTCTATTTTGTCAGAAATTAATATTGGTACTCCTGCTCTTTTTTGCTTATTGTTTGCTTGATATATTTTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFF::FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF,FFFFFFFF:FFFFNM:i:5MD:Z:53A40C7C0T15A18MC:Z:151MAS:i:113XS:i:66Yt:Z:UU A00126:224:H7HMWDSX3:3:1103:14299:3286381Test_seq17034660151M=170143-354TATAGATGGATCGTGTTTCTTTATCCAGTCCGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTTTTCATGTGTGTTGTTGGCCATTTCATFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFNM:i:1MD:Z:144T6MC:Z:13S138MAS:i:146XS:i:48Yt:Z:UU 60 60 GAACAGTGATGAATGATCAATTGTCCCTTTTATCATTATATAGTGTCCTTCTTTATCCTTTGTGGCGGATTTAAGTCTAAAGTCTATTTTGTCAGAAATTAATATTGGTACTCCTGCTCTTTTTTGCTTATTGTTTGCTTGATATATTTTT TATAGATGGATCGTGTTTCTTTATCCAGTCCGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTTTTCATGTGTGTTGTTGGCCATTTCAT +A00126:224:H7HMWDSX3:3:1103:8558:33066 Test_seq 167149 Test_seq 167536 + - UU A00126:224:H7HMWDSX3:3:1103:8558:3306697Test_seq16714942151M=167404388ATTTGCATTTCTCTAATGGCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAAFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF,FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FF,FFFFFFFFF:FFFF:,F:FFFF:FFFFFFFFFFFFNM:i:1MD:Z:94T56MC:Z:133M18SAS:i:146XS:i:126XA:Z:Test_seq,-213735,151M,5;Yt:Z:UU A00126:224:H7HMWDSX3:3:1103:8558:33066145Test_seq16740460133M18S=167149-388GAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCGGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATACCTTGTATTAGGGCTCCTAGGGTTGTCCCAATTTTTTCTTCCATGATCTAAAGCAATCTTTTGCAGCFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:6MD:Z:38T44G2C22A0T2T19MC:Z:151MAS:i:103XS:i:71Yt:Z:UU 42 60 ATTTGCATTTCTCTAATGGCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAA GAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCGGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATACCTTGTATTAGGGCTCCTAGGGTTGTCCCAATTTTTTCTTCCATGATCTAAAGCAATCTTTTGCAGC +A00126:224:H7HMWDSX3:3:1103:12463:35008 ! 0 Test_seq 137147 - + NR A00126:224:H7HMWDSX3:3:1103:12463:35008145Test_seq13748460124M27S=137147-461GCTTTCAGCCAAGATCCATCTGACATCAGCAGTGACCTCCCTCATTCCACGTTCTCTTCTAAACCCGGCTTGAATTTCTGGCAGTTCCCTGTTGATGTACCGCTGTAACATTTTTTGAACGATCGATCATTTATCTAAAATTATCTTTATTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:124MC:Z:151MAS:i:124XS:i:32Yt:Z:NR A00126:224:H7HMWDSX3:3:1103:12463:3500897Test_seq13714760151M=137484461GCATATTTCAGATTGTTAATGAGCCCTCCTCCAGTCTTGATGCTGCATTCTTCATATTATCCAGCTTCTCCGACTATTTGCTCAGCATACAGATTGAATAAGTATCGTAAAAGGATATAACCCTGATATAACCTTTCCTGATTTTAAACTAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:124M27SAS:i:151XS:i:33Yt:Z:NR 0 60 GCATATTTCAGATTGTTAATGAGCCCTCCTCCAGTCTTGATGCTGCATTCTTCATATTATCCAGCTTCTCCGACTATTTGCTCAGCATACAGATTGAATAAGTATCGTAAAAGGATATAACCCTGATATAACCTTTCCTGATTTTAAACTA +A00126:224:H7HMWDSX3:3:1103:9697:35008 Test_seq 167173 Test_seq 167658 + - UU A00126:224:H7HMWDSX3:3:1103:9697:3500897Test_seq16717346151M=167508486GATCGGGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGATTCATGTAAATTTTAGAGATCAGGCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFF:FFFFFFFFFF:F,FFF,,F,FF:FFFFFFFF:FFFFF,F:FFFF:FFFFFFFFFF:FF:FFFNM:i:5MD:Z:5A64T55A7G15A0MC:Z:151MAS:i:130XS:i:106XA:Z:Test_seq,-213711,151M,9;Yt:Z:UU A00126:224:H7HMWDSX3:3:1103:9697:35008145Test_seq16750860151M=167173-486GGTTGATCCTATTTTTTCTTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGGTATGCCAGCACCATFFFFF:FFFFFFFF:F:FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFFNM:i:1MD:Z:136T14MC:Z:151MAS:i:146XS:i:114Yt:Z:UU 46 60 GATCGGGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGATTCATGTAAATTTTAGAGATCAGGC GGTTGATCCTATTTTTTCTTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGGTATGCCAGCACCAT +A00126:224:H7HMWDSX3:3:1103:4282:36057 ! 0 Test_seq 169466 - - NR A00126:224:H7HMWDSX3:3:1103:4282:36057161Test_seq1690005327S124M=169316467AACTCCACATAAGGCAGATATTATAACTCTTCTCTGAAAGTTTGGTAGAACTCTGCAGTGAAGCCGTCCGGGCCAGGGCTTTTTTTTGTTGGGAGTTTTTTGATTACCGTTTCAATCTCTTTTTTTGTTATGGGTCTATTTAGTTGTTCTAFFFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFF:F:FFFFFFFFFFFFFFFFF:FFFFFFFFFF,F:F:::FFFFFFF,:F,FFFFF,,FFFF:FFFFFFFNM:i:4MD:Z:11G29T8A14A58MC:Z:151MAS:i:104XS:i:78Yt:Z:NR A00126:224:H7HMWDSX3:3:1103:4282:3605781Test_seq16931660151M=169000-467TAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAAGTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGFFFFFFFFFFFFFF::FFFFFF:FF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFF:FFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFNM:i:1MD:Z:36T114MC:Z:27S124MAS:i:146XS:i:107Yt:Z:NR 0 60 TAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAAGTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGG +A00126:224:H7HMWDSX3:3:1103:19768:36573 Test_seq 166082 Test_seq 166567 + - UU A00126:224:H7HMWDSX3:3:1103:19768:3657397Test_seq16608260151M=166417486CCTCAGGAGTTGTCTGTTGTGCTCCCTAGCAGGGCAGACATCGATTGTGGCCGGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCTTAGTTGTCGTGTGGCCTTGGTGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF,F:FFFFFFFFFFFFFF,FFF:FNM:i:2MD:Z:27G0A122MC:Z:151MAS:i:141XS:i:69Yt:Z:UU A00126:224:H7HMWDSX3:3:1103:19768:36573145Test_seq16641760151M=166082-486TGCTCCGCTGACCTTTGAAGCTTTCATTTTATCCCGGAAACCTCTTTGCTTTTAGTCCAGTCCAATTAGGCTGACCTTCCTTGTATTGAGTGTTGTCTTTCCCTTCACCCAAAGCAGTTCTTATCTACTGATTGATCAATAAAAAACCCTCFFFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:88T62MC:Z:151MAS:i:146XS:i:40Yt:Z:UU 60 60 CCTCAGGAGTTGTCTGTTGTGCTCCCTAGCAGGGCAGACATCGATTGTGGCCGGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCTTAGTTGTCGTGTGGCCTTGGTG TGCTCCGCTGACCTTTGAAGCTTTCATTTTATCCCGGAAACCTCTTTGCTTTTAGTCCAGTCCAATTAGGCTGACCTTCCTTGTATTGAGTGTTGTCTTTCCCTTCACCCAAAGCAGTTCTTATCTACTGATTGATCAATAAAAAACCCTC +A00126:224:H7HMWDSX3:3:1104:23149:1501 Test_seq 170256 Test_seq 170649 + - UU A00126:224:H7HMWDSX3:3:1104:23149:150197Test_seq17025660151M=170511394CTTATTGTTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCGTGACTCTCTGTCTCTTTATTGGTGCATTTFF:F,,F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:F:FFF:FF,FFFFFFFFF,FFFFFFFFFFFF:FFFFFFF,F:,FFFFFFFFFFFFFFFF,FFFFFFFFFFFFF:FNM:i:1MD:Z:6A144MC:Z:87M12I52MAS:i:146XS:i:79Yt:Z:UU A00126:224:H7HMWDSX3:3:1104:23149:1501145Test_seq1705116087M12I52M=170256-394TTTTGTGCTGAGACGTTTTTCTTAGTAAATTGTGAGATCCTCATTTTCATAGTGTTTGACTTTATGTTAGTTGAGTCGTTACATTTTTCTTGGCTTTTATCTTGAGTTATGGAGTTGTTATACCTTTTTGTGGTTACCTTATTATTTACCCFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:20MD:Z:27G23A5A10T13G18A3A3T29MC:Z:151MAS:i:81XS:i:25Yt:Z:UU 60 60 CTTATTGTTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCGTGACTCTCTGTCTCTTTATTGGTGCATTT TTTTGTGCTGAGACGTTTTTCTTAGTAAATTGTGAGATCCTCATTTTCATAGTGTTTGACTTTATGTTAGTTGAGTCGTTACATTTTTCTTGGCTTTTATCTTGAGTTATGGAGTTGTTATACCTTTTTGTGGTTACCTTATTATTTACCC +A00126:224:H7HMWDSX3:3:1104:5339:2096 Test_seq 169382 Test_seq 169468 + - UU A00126:224:H7HMWDSX3:3:1104:5339:209697Test_seq1693826087M=16938287TAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCCCTAATTTTTATTATTTGTTTTCTTCGGGTGCCTGATGGATFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:3MD:Z:46T25T12G1MC:Z:87MAS:i:75XS:i:46Yt:Z:UU A00126:224:H7HMWDSX3:3:1104:5339:2096145Test_seq1693826087M=169382-87TAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGATF:FFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:85G1MC:Z:87MAS:i:85XS:i:56Yt:Z:UU 60 60 TAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCCCTAATTTTTATTATTTGTTTTCTTCGGGTGCCTGATGGAT TAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGAT +A00126:224:H7HMWDSX3:3:1104:25093:3615 ! 0 Test_seq 16111 - + NR A00126:224:H7HMWDSX3:3:1104:25093:361581Test_seq1633360127M24S=16111-349TGTAATCGCCATTTATATTGGTGAAAGAAGGTATTTTCAATGAATAAGTTTTTGGTCTTGCAAAATTCTATCATTCAATCTTTGGCATTGTTTCTATCACCAAGGCCATATTTTCCAACCACTGATCGATCATCTGTTCATGTGCTTATTAFFFFFFFF:FFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFNM:i:0MD:Z:127MC:Z:151MAS:i:127XS:i:55Yt:Z:NR A00126:224:H7HMWDSX3:3:1104:25093:3615161Test_seq1611160151M=16333349CTAAATTCAGATGGGATATACTCAAGGTCATATTTTGGCTCTTGTGGACTTGCTCTGATTTTCGTCAGTATCAGCGTGAACTTGCATATGAGTAATTGATGGTCTGTTCCACAGTCGGCCCCTAGCCTTGTTTTGACTGATGATACCGAGTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:127M24SAS:i:151XS:i:0Yt:Z:NR 0 60 CTAAATTCAGATGGGATATACTCAAGGTCATATTTTGGCTCTTGTGGACTTGCTCTGATTTTCGTCAGTATCAGCGTGAACTTGCATATGAGTAATTGATGGTCTGTTCCACAGTCGGCCCCTAGCCTTGTTTTGACTGATGATACCGAGT +A00126:224:H7HMWDSX3:3:1104:32714:5165 Test_seq 238200 Test_seq 238677 + - UU A00126:224:H7HMWDSX3:3:1104:32714:516597Test_seq23820060151M=238527478ATGGTTGAGGGAGCTTGTGAGGGTGAGCACTGGGGGGAGGACACGGTAGGTCACCAAGCACAGAAAGCACCCTTCATTTTTGTAAAAATATGGTAATCTTTTGTTGTATTAAGTCACTGAGCTGTCGGGATTGTTACTGCAGCACAACCTCFFFFFFFFFFF,FFFFFFFFFFFFFFFFFF,FFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FF:,,FFFFFFFFFFF::FFF,,FF,F:FF::FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1104:32714:5165145Test_seq23852760151M=238200-478TACAACGTCAATTTTATAACTGCATAGGAATAATAACCTATTATTTGGATGTGCCACGCATTACTCAACAGTTCCTCATTGTTAGACCTTTATTTTTCCTGTTTTTCATGTTGTGATTAACATTGTTGATGATAATCTTTGCATACGTTCT:FF,FFFF:F,FFFF:F:FFFFFF,FFFFFFFFF::FFFF,F,F,FFFFFFFFFFFFFFF:FFFFF:FFFF,FFFFFF:::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFF::FF:F:FFFFF:FFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU 60 60 ATGGTTGAGGGAGCTTGTGAGGGTGAGCACTGGGGGGAGGACACGGTAGGTCACCAAGCACAGAAAGCACCCTTCATTTTTGTAAAAATATGGTAATCTTTTGTTGTATTAAGTCACTGAGCTGTCGGGATTGTTACTGCAGCACAACCTC TACAACGTCAATTTTATAACTGCATAGGAATAATAACCTATTATTTGGATGTGCCACGCATTACTCAACAGTTCCTCATTGTTAGACCTTTATTTTTCCTGTTTTTCATGTTGTGATTAACATTGTTGATGATAATCTTTGCATACGTTCT +A00126:224:H7HMWDSX3:3:1104:15076:6026 Test_seq 171233 Test_seq 171678 + - UU A00126:224:H7HMWDSX3:3:1104:15076:602697Test_seq17123360151M=171528446TCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATGGGGTTCGATGAGCATCTTGGATAGATCTCCTTTCGTCTTTCATGATGTCAGGGAAGTTTTGTGTCAGGAGTTCTTCAACTATTTFF,FFFFFFFFFFF:FFFFFFFFFFFF:F:FFFFF,FFFFFF:,FFFFFFFFFFFFFFFFFF,FFFFFFFFF:FFFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:FFFFFFFF:F:FF:F,FFFFFFFF,F,FFNM:i:1MD:Z:93A57MC:Z:151MAS:i:146XS:i:38Yt:Z:UU A00126:224:H7HMWDSX3:3:1104:15076:6026145Test_seq17152860151M=171233-446TGAGTCCCTGGTCCTCCAGATGTCCCAGTCTACATTCTAATTGCTCGAGTCTGCTCCTCTGACTTCCTATTGCGTTGTCTAATTCTGTAATTTTATTGTTAATCTTTTGGATTTCTATATGCTGTCTCTCTATGGATTCTTGCAACTTATTFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FF,FF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:FNM:i:5MD:Z:3T16A34T9T51C33MC:Z:151MAS:i:127XS:i:60Yt:Z:UU 60 60 TCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATGGGGTTCGATGAGCATCTTGGATAGATCTCCTTTCGTCTTTCATGATGTCAGGGAAGTTTTGTGTCAGGAGTTCTTCAACTATTT TGAGTCCCTGGTCCTCCAGATGTCCCAGTCTACATTCTAATTGCTCGAGTCTGCTCCTCTGACTTCCTATTGCGTTGTCTAATTCTGTAATTTTATTGTTAATCTTTTGGATTTCTATATGCTGTCTCTCTATGGATTCTTGCAACTTATT +A00126:224:H7HMWDSX3:3:1104:6325:6997 Test_seq 170855 Test_seq 171213 + - UU A00126:224:H7HMWDSX3:3:1104:6325:6997161Test_seq17085560151M=171063359TGCTTTCCCTATTTGAGTTGCGTTGATATCAGGACGTTCTGTTTTGTGACCTTGTATTGTGCTGGTACCTGATATTATTGGTCATCAGGCCAAACAATCTCCTTTAGCATTTCTTGCAGTCTTGGTTTAGTTTTTGCAAATTCTCTAAACTFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:FFF,FF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:F:FFFFFF:FFFFFNM:i:1MD:Z:104A46MC:Z:151MAS:i:146XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1104:6325:699781Test_seq17106360151M=170855-359GATATATGATCCTTGGTTGGCAGTTTTTCTCGTTCAGTGCTCTGTATACGTCGTCTCATTCCCTTCTTGCCTGCATGGTTTCTGCTGAGTAATCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCTCTGGCTGCFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:5MD:Z:28A2C23C35G50C8MC:Z:151MAS:i:126XS:i:58Yt:Z:UU 60 60 TGCTTTCCCTATTTGAGTTGCGTTGATATCAGGACGTTCTGTTTTGTGACCTTGTATTGTGCTGGTACCTGATATTATTGGTCATCAGGCCAAACAATCTCCTTTAGCATTTCTTGCAGTCTTGGTTTAGTTTTTGCAAATTCTCTAAACT GATATATGATCCTTGGTTGGCAGTTTTTCTCGTTCAGTGCTCTGTATACGTCGTCTCATTCCCTTCTTGCCTGCATGGTTTCTGCTGAGTAATCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCTCTGGCTGC +A00126:224:H7HMWDSX3:3:1104:21658:7279 ! 0 Test_seq 293960 - - NR A00126:224:H7HMWDSX3:3:1104:21658:727997Test_seq2935986032S119M=293810363ACAAAAACATACTGTGAAAACGAGTCTAGAGTAATCCTAAACCTATACTTATGAATGTCATCCTGTCTGGAAATAGAGTTTTCTTTGTTATGTTAATGAGGTCATATCAGTGCCCTAAACCCAAGCTTTTGTGAGTTACAAGGAGAAGCATFFFFF::FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF,FF,FFFFFFFFFFFFF,FFFF::FFFFFFFFFFF,FFFF::FFFFFFFFFFFFFFFFFFF:,FF,FFF:FFFFFFFF,FFFF:F,FFFNM:i:0MD:Z:119MC:Z:151MAS:i:119XS:i:0Yt:Z:NR A00126:224:H7HMWDSX3:3:1104:21658:7279145Test_seq29381060151M=293598-363GAAAAAGACCAGAATCCTCCTCCACTGTCAAAAAGGAGCCTTCCCCTGGAGCAGGAGCCCTGAATTAGGACTTCCAGCCTCCAGACCAGTGAGAAAATAAATTTCTCTTCTTTAAAGCCCCCACTTGCAGTATTTCTGTTACAGCAGCACTFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFF:FFFFFFF,FFFFFF::FFFFFFFFFFFFF,FFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFF,FFFFFFFFFF:FFFFFFFFFFFFFFFFFFNM:i:1MD:Z:85A65MC:Z:32S119MAS:i:146XS:i:0Yt:Z:NR 0 60 GAAAAAGACCAGAATCCTCCTCCACTGTCAAAAAGGAGCCTTCCCCTGGAGCAGGAGCCCTGAATTAGGACTTCCAGCCTCCAGACCAGTGAGAAAATAAATTTCTCTTCTTTAAAGCCCCCACTTGCAGTATTTCTGTTACAGCAGCACT +A00126:224:H7HMWDSX3:3:1104:8169:7498 ! 0 Test_seq 167708 - - NR A00126:224:H7HMWDSX3:3:1104:8169:749897Test_seq167315827S124M=167558394GCCCTCCTGTGGCCAGAAAAATCTCTCAGATCAGGCGCTGGTCTGAGATGTCATAGCTGAATATTCTTTCCCAGTCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFF::FFFFF:,FFFFFFFFFFF,FFFFFFFFFFFFFF,FNM:i:2MD:Z:8A44A70MC:Z:151MAS:i:114XS:i:109XA:Z:Test_seq,-213596,124M27S,3;Test_seq,+400736,27S124M,5;Yt:Z:NR A00126:224:H7HMWDSX3:3:1104:8169:7498145Test_seq16755831151M=167315-394TTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATCTTTTCCCCAATTGACTGACACTGGTCCTTTGTCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:118A32MC:Z:27S124MAS:i:146XS:i:131XA:Z:Test_seq,+213330,130M2I19M,4;Yt:Z:NR 0 31 TTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATCTTTTCCCCAATTGACTGACACTGGTCCTTTGTC +A00126:224:H7HMWDSX3:3:1104:5791:7921 ! 0 Test_seq 171294 - - NR A00126:224:H7HMWDSX3:3:1104:5791:7921161Test_seq1710696024S127M=171144226ACTATTCTAGCTATCAGCAAGGATTGATCTTTGGTTGGCAGTTTTTCTCGTTCAGTGCTCTGTATACGTCGTCCCATTCCCTTCTTGCCTGCATGGTTTCTGCTGAGTAGTCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTFFFFFFFFFFFFFFFFFFFFFFF:FFFF::FFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFNM:i:3MD:Z:5C16A2C101MC:Z:151MAS:i:112XS:i:68Yt:Z:NR A00126:224:H7HMWDSX3:3:1104:5791:792181Test_seq17114460151M=171069-226CTGCTGAGTAGTCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGTTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:66C84MC:Z:24S127MAS:i:146XS:i:55Yt:Z:NR 0 60 CTGCTGAGTAGTCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGTTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTT +A00126:224:H7HMWDSX3:3:1104:11568:9377 Test_seq 116069 Test_seq 168405 - + UR A00126:224:H7HMWDSX3:3:1104:11568:93772193Test_seq16865160126M25H=168405-372CTTTTCTGCATCTATTGATAAGATCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFF,FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:65T60MC:Z:151MAS:i:121XS:i:55SA:Z:Test_seq,116047,-,128S23M,21,0;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1104:11568:9377145Test_seq11604721128S23M=16840552337CTTTTCTGCATCTATTGATAAGATCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCGATCACAAAATGGAGGACAACTACAFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFF,FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFNM:i:0MD:Z:23MC:Z:151MAS:i:23XS:i:19SA:Z:Test_seq,168651,-,126M25S,60,1;XA:Z:Test_seq,-85506,127S19M5S,0;Yt:Z:UR A00126:224:H7HMWDSX3:3:1104:11568:937797Test_seq16840560151M=116047-52337TATTTCTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFF,FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF,:FF:FFFFFFFNM:i:1MD:Z:90T60MC:Z:128S23MAS:i:146XS:i:81Yt:Z:UR 21 60 CTTTTCTGCATCTATTGATAAGATCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCGATCACAAAATGGAGGACAACTACA TATTTCTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTT +A00126:224:H7HMWDSX3:3:1104:24921:11741 Test_seq 168602 Test_seq 212800 - - UU A00126:224:H7HMWDSX3:3:1104:24921:11741177Test_seq16845260151M=21265044199TGTTGAATAAGAGCAGTGATAAAGGGCATCCTTGTCTGGTTCCTGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATF:FFFFFFFFFF,FFFFFFF,,:,:,FFFFFFFFFFF,:FF:FFFFFFFFFFF:FF:FFFFFFFFF::FFFF:F:FFFFFFFFFFFFFFFFFF:FFFFFF::FFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFF::F:FFFF:FFFFNM:i:1MD:Z:14G136MC:Z:151MAS:i:146XS:i:93Yt:Z:UU A00126:224:H7HMWDSX3:3:1104:24921:11741113Test_seq21265024151M=168452-44199GCAAGGAAGATTTAAAGTTATCACTTTTTGCAGATGACATGATTATATACACAGAAAACCCTAAGGAATCCTCCAGAAAACTACTGAAACTAATAGAAGAGTTTGGCAGAGTCTCTGGTTATAAAATAAACATACAAAAATCACTTGGATTF,,,,F,,FF,,:FFF,F,FF,:::,FFF,:FF,FFFFFFF:FFFFF:FF,:F::,F,:FF,,:,:,:FF::FFF,:F:F,FF,:F:F,:::F,,:,FF,:FFF:FFF::,FFFFF,FFF:F,:FFFFF:FFF:F,FFF,FFF,FFF:FFFNM:i:11MD:Z:7G2A0G4A5T2A17C0T17T52A8G26MC:Z:151MAS:i:100XS:i:85XA:Z:Test_seq,+168238,151M,14;Yt:Z:UU 60 24 TGTTGAATAAGAGCAGTGATAAAGGGCATCCTTGTCTGGTTCCTGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTAT GCAAGGAAGATTTAAAGTTATCACTTTTTGCAGATGACATGATTATATACACAGAAAACCCTAAGGAATCCTCCAGAAAACTACTGAAACTAATAGAAGAGTTTGGCAGAGTCTCTGGTTATAAAATAAACATACAAAAATCACTTGGATT +A00126:224:H7HMWDSX3:3:1104:8323:13463 Test_seq 170316 Test_seq 170786 + - UU A00126:224:H7HMWDSX3:3:1104:8323:13463161Test_seq17031660151M=170636471AAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAGTAAGTTTTTAGTGCTGTCATTTTGATGFFFFFFFFFFFFFFFFFF::FFFFFFFFFF:FFFFFFFFFFFFFFFF:FF:FFFFFF:FF:FFFFFFFFFFFFFFFF,FFFFF:FFFFFFFFFFF,FF:FFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFF:FFFF:FFFFFFFF,FFFNM:i:1MD:Z:123A27MC:Z:151MAS:i:146XS:i:52Yt:Z:UU A00126:224:H7HMWDSX3:3:1104:8323:1346381Test_seq17063660151M=170316-471CTTTTTATTTACCCCTATTTTTCTAAGTAAAAACCTAACTTGTATCCTTCTATTTCGCCTTGTATCACTCTCCATCTGGCAGTTCAATGCCTCCTATATTTAGTCCCTCTTTTTGATTATTTTGATCGTTTATCTATTGATTTCCATGATTFFFFFFFFFF:FFFFFFFFFFFFFFF,FFFFFFFFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF,FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:F,FFFFFFFFFFFFFFFFFFFFFFNM:i:7MD:Z:3A42G2G3A2A38G25G29MC:Z:151MAS:i:117XS:i:23Yt:Z:UU 60 60 AAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAGTAAGTTTTTAGTGCTGTCATTTTGATG CTTTTTATTTACCCCTATTTTTCTAAGTAAAAACCTAACTTGTATCCTTCTATTTCGCCTTGTATCACTCTCCATCTGGCAGTTCAATGCCTCCTATATTTAGTCCCTCTTTTTGATTATTTTGATCGTTTATCTATTGATTTCCATGATT +A00126:224:H7HMWDSX3:3:1104:18855:14418 ! 0 Test_seq 171064 - + NR A00126:224:H7HMWDSX3:3:1104:18855:1441881Test_seq17129360129M22S=171064-358TTAAATGGGGTTCGATGAGCATCTTGGATAGATATCCTTTCGTCTTTCATGATGTCAGGGAAGTTTTGTGTCAGGAGTTCTACAACTATTTTCTCTGTGTTTTCTGTCCCCCCTCCCTGTTCTGGGACTACTCATGGAGACCCCATGTTACFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:3MD:Z:81T25G15T5MC:Z:151MAS:i:114XS:i:0Yt:Z:NR A00126:224:H7HMWDSX3:3:1104:18855:14418161Test_seq17106460151M=171293358ATATATGATCCTTGGTTGGCAGTTTTTCTCCTTCAGTGCTCTGTATACGTCGTCCCATTCCCTTCTTGCCTGCATGGTTTCTGCTGAGTAGTCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF:FFFFFFFFFFFFFFNM:i:1MD:Z:27A123MC:Z:129M22SAS:i:146XS:i:73Yt:Z:NR 0 60 ATATATGATCCTTGGTTGGCAGTTTTTCTCCTTCAGTGCTCTGTATACGTCGTCCCATTCCCTTCTTGCCTGCATGGTTTCTGCTGAGTAGTCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCT +A00126:224:H7HMWDSX3:3:1104:5828:14622 Test_seq 155716 Test_seq 156138 + - UU A00126:224:H7HMWDSX3:3:1104:5828:14622161Test_seq15571660151M=156003423TTTCTTCATTTTTTTTAATTCTTTTATCTGATTTTTCTTCAAATGTATTAGGGCCAAGTGATTTATGTTCAATTTCAGAAATTCTAGCTTCTACTTGCTCAATTCTGCTCCTCTGACTTTCTATTGAGTTGTCTACTTCTGTAATTTTATT:,:F:FFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFF,FFFF,FFFFFFFFF,FFFFFF,FF:FFFFFFFF:FFFFFFF,,FFF,FFFFF:FFFFFFF,FF:FF:FNM:i:0MD:Z:151MC:Z:136M15SAS:i:151XS:i:97Yt:Z:UU A00126:224:H7HMWDSX3:3:1104:5828:1462281Test_seq15600360136M15S=155716-423TCCCTGATTTCCTTCCTGATGTCTTGAAGGGTTCTGTATATTAATCTTTTGTATTCTGTATCTGATAATTCCAGGAATGCACTTTCATCTAGAAGATCCCTAGATTTGTTGTTTTGAGAGCTTGTTGAGGTGATCACTCCAGGACCTTTGCF:,FF,F,:FF:,F:FFFFFFF:FFFFFFFFFFFFFF,:,F,F:,FFFFFF,,FF:FFF:FFFF,FFFF::FFFF,FFFFFFFFFFFFFF,:F:FFFFFFFFFFFFFFFFFF:FF:FFFFFFF:FFFFFFFFFFF:F::F:FFFFFFFFFFNM:i:2MD:Z:5C38A91MC:Z:151MAS:i:126XS:i:62Yt:Z:UU 60 60 TTTCTTCATTTTTTTTAATTCTTTTATCTGATTTTTCTTCAAATGTATTAGGGCCAAGTGATTTATGTTCAATTTCAGAAATTCTAGCTTCTACTTGCTCAATTCTGCTCCTCTGACTTTCTATTGAGTTGTCTACTTCTGTAATTTTATT TCCCTGATTTCCTTCCTGATGTCTTGAAGGGTTCTGTATATTAATCTTTTGTATTCTGTATCTGATAATTCCAGGAATGCACTTTCATCTAGAAGATCCCTAGATTTGTTGTTTTGAGAGCTTGTTGAGGTGATCACTCCAGGACCTTTGC +A00126:224:H7HMWDSX3:3:1104:5719:17409 Test_seq 167317 Test_seq 214049 - - UU A00126:224:H7HMWDSX3:3:1104:5719:17409113Test_seq16716752151M=21389946733GCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAATCATGTAGATTTTAGAGAF:FFFFFFF:F,FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:76T74MC:Z:151MAS:i:146XS:i:121XA:Z:Test_seq,+213717,151M,6;Yt:Z:UU A00126:224:H7HMWDSX3:3:1104:5719:17409177Test_seq21389914151M=167167-46733AGATTCCATCTCACACCAGCAAGGCTGGCATTAATCCAAAAAACAGAAAATAATAAATGTTGGAGAGGCTGCGGAGAGATTGGAACTCTCATACACTGCTGGTGGGAATGTAAAATGGTACAACCACTTTGGAAATCTATCTGGCGTTATCFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFNM:i:7MD:Z:14T3A26C33C9T54T3T2MC:Z:151MAS:i:119XS:i:111XA:Z:Test_seq,+166985,151M,8;Test_seq,+400413,7S144M,9;Yt:Z:UU 52 14 GCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAATCATGTAGATTTTAGAGA AGATTCCATCTCACACCAGCAAGGCTGGCATTAATCCAAAAAACAGAAAATAATAAATGTTGGAGAGGCTGCGGAGAGATTGGAACTCTCATACACTGCTGGTGGGAATGTAAAATGGTACAACCACTTTGGAAATCTATCTGGCGTTATC +A00126:224:H7HMWDSX3:3:1104:27796:17879 Test_seq 168874 Test_seq 169111 + - UU A00126:224:H7HMWDSX3:3:1104:27796:17879161Test_seq16887460151M=168961238TTTTCTTTTTTTGTAATGTTTTTACCTGGTTTTGGTATCAGGGAGATGGTAGCTTCATAGAATGAGTTGGGTAGTATTCCGTCTTTTTCTATGCTTTGAAATACCTTCAGTAGTAGTGGTGTTAAGTCTTCTCTGAAGGTTTGGTAGAACTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:FFFFNM:i:4MD:Z:19C20A9G34G65MC:Z:151MAS:i:131XS:i:71Yt:Z:UU A00126:224:H7HMWDSX3:3:1104:27796:1787981Test_seq16896160151M=168874-238TCTATGCTTTGAAATACCTTCAGTAGTAGTGGTGTTAAGTCTTCTCTGAAGGTTTGGTAGAACTCTGCAGTGAAGCCGTCCGGGCCAGGACTTTTTTTTGTTGGAAGTTTTTTGATTACCGTTTCAATCTCTTTTTTTGTTATGGGTCTATFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:80T70MC:Z:151MAS:i:146XS:i:81Yt:Z:UU 60 60 TTTTCTTTTTTTGTAATGTTTTTACCTGGTTTTGGTATCAGGGAGATGGTAGCTTCATAGAATGAGTTGGGTAGTATTCCGTCTTTTTCTATGCTTTGAAATACCTTCAGTAGTAGTGGTGTTAAGTCTTCTCTGAAGGTTTGGTAGAACT TCTATGCTTTGAAATACCTTCAGTAGTAGTGGTGTTAAGTCTTCTCTGAAGGTTTGGTAGAACTCTGCAGTGAAGCCGTCCGGGCCAGGACTTTTTTTTGTTGGAAGTTTTTTGATTACCGTTTCAATCTCTTTTTTTGTTATGGGTCTAT +A00126:224:H7HMWDSX3:3:1104:10926:18349 Test_seq 170851 Test_seq 171176 + - UU A00126:224:H7HMWDSX3:3:1104:10926:18349161Test_seq17085160151M=171045326TTTGTGCTTTCCCTATTTGAGTTGCGTTGATATCAGGACGTTCTGTTTTGTGACCTTGTATTGTGCTGGTACCTGATATTATTGGTCATCAGGCCAAACAATCTCCTTAAGCATTTCTTGCAGTCTTGGTTTAGTTTTTGCAAATTCTCTAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFF,FF:F:FNM:i:0MD:Z:151MC:Z:132M19SAS:i:151XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1104:10926:1834981Test_seq17104560132M19S=170851-326TCAGAGAGAGTTTTGCTGGATATATGATCCTTGGTTGGCAGTTTTTCTCCTTCAGTGCTCTGTATACGTCGTCCCATTCCCTTCTTGATTGCATGGTTTCTGCTGAGTAGTCTGAACTTATTCTTATTGATTAATCTCTCATGTAACCTTTFFFFFFFFFFF,FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:4MD:Z:2C43A40C0C43MC:Z:151MAS:i:114XS:i:65Yt:Z:UU 60 60 TTTGTGCTTTCCCTATTTGAGTTGCGTTGATATCAGGACGTTCTGTTTTGTGACCTTGTATTGTGCTGGTACCTGATATTATTGGTCATCAGGCCAAACAATCTCCTTAAGCATTTCTTGCAGTCTTGGTTTAGTTTTTGCAAATTCTCTA TCAGAGAGAGTTTTGCTGGATATATGATCCTTGGTTGGCAGTTTTTCTCCTTCAGTGCTCTGTATACGTCGTCCCATTCCCTTCTTGATTGCATGGTTTCTGCTGAGTAGTCTGAACTTATTCTTATTGATTAATCTCTCATGTAACCTTT +A00126:224:H7HMWDSX3:3:1104:7075:20478 ! 0 Test_seq 171148 - + NR A00126:224:H7HMWDSX3:3:1104:7075:20478145Test_seq17129360129M22S=171148-274TTAAATGGGGTTCGATGAGCATCTTGGATAGATATCCTTTCATCTTTCATGATGTCAGGGAAGTTTTGTGTCAGGAGTTCTTCAACTATTTTCTCTGTGTTTTCTGTCCCCCCTCCCTGTTCTGGGACTACTCCAGCTGTAGTTGATAGCAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFNM:i:3MD:Z:41G65G15T5MC:Z:151MAS:i:114XS:i:48Yt:Z:NR A00126:224:H7HMWDSX3:3:1104:7075:2047897Test_seq17114860151M=171293274TGAGTAGTCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCATTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFNM:i:1MD:Z:66T84MC:Z:129M22SAS:i:146XS:i:51Yt:Z:NR 0 60 TGAGTAGTCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCATTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAAT +A00126:224:H7HMWDSX3:3:1104:7229:20525 ! 0 Test_seq 171148 - + NR A00126:224:H7HMWDSX3:3:1104:7229:20525145Test_seq17129360129M22S=171148-274TTAAATGGGGTTCGATGAGCATCTTGGATAGATATCCTTTCATCTTTCATGATGTCAGGGAAGTTTTGTGTCAGGAGTTCTTCAACTATTTTCTCTGTGTTTTCTGTCCCCCCTCCCTGTTCTGGGACTACTCCAGCTGTAGTTGATAGCAFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:3MD:Z:41G65G15T5MC:Z:151MAS:i:114XS:i:48Yt:Z:NR A00126:224:H7HMWDSX3:3:1104:7229:2052597Test_seq17114860151M=171293274TGAGTAGTCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCATTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFNM:i:1MD:Z:66T84MC:Z:129M22SAS:i:146XS:i:51Yt:Z:NR 0 60 TGAGTAGTCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCATTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAAT +A00126:224:H7HMWDSX3:3:1104:27416:21355 ! 0 Test_seq 167500 - - NR A00126:224:H7HMWDSX3:3:1104:27416:21355161Test_seq1671734323S128M=167350328CCCTAATGTAATCCCTAACCTTAGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCCTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAATFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFFFFFF,,F:FFFFF:FFFFFF:FF,FFFF,FNM:i:2MD:Z:47T22T57MC:Z:151MAS:i:118XS:i:98XA:Z:Test_seq,-213734,128M23S,6;Yt:Z:NR A00126:224:H7HMWDSX3:3:1104:27416:2135581Test_seq1673507151M=167173-328ATTCTTTCCCAGTCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATGCCCTGTATTAGGGFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFNM:i:1MD:Z:18A132MC:Z:23S128MAS:i:146XS:i:141XA:Z:Test_seq,+213534,151M,2;Yt:Z:NR 0 7 ATTCTTTCCCAGTCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATGCCCTGTATTAGGG +A00126:224:H7HMWDSX3:3:1104:28411:22075 Test_seq 203500 Test_seq 214751 + + RU A00126:224:H7HMWDSX3:3:1104:28411:22075129Test_seq20350060151M=21475111252GATCCTGTTTTCTGTCTCTCTCTCTCTCTCTGTGTGTATGTGTGTGTGTTTTCCCGAGAAATTACCAATTAGTAAGATTTTCAGAATTTAGTTTGGTATTTATTTGTTTTTGTTTTTTATTTGTAATGTAACCGGAATAAGGTTCTTGCCTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFF:FFFFFFF,FFF,F:,::FFFFF,FFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:37M114SAS:i:151XS:i:0Yt:Z:RU A00126:224:H7HMWDSX3:3:1104:28411:2207565Test_seq2147512737M114S=203500-11252CTAAGATGCATCAATTGCTCTCAACCCACCTGGATCACTCTTTTCCCACCCAAGAACTTTTAAAAACTAAGGCCCATCTTTTCTTCTGTGAAACAGGGGTAAGCACTGCTCTAGGCCCTCCTCGTCTCCACTTGCAAGCCTCTCCAATAAAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF,FFFFNM:i:0MD:Z:37MC:Z:151MAS:i:37XS:i:32SA:Z:Test_seq,203705,-,115M36S,60,0;XA:Z:Test_seq,-399667,114S37M,1;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1104:28411:220752129Test_seq20370560115M36H=203500-320TTTATTGGAGAGGCTTGCAAGTGGAGACGAGGAGGGCCTAGAGCAGTGCTTACCCCTGTTTCACAGAAGAAAAGATGGGCCTTAGTTTTTAAAAGTTCTTGGGTGGGAAAAGAGTFFFF,FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:115MC:Z:151MAS:i:115XS:i:0SA:Z:Test_seq,214751,+,37M114S,27,0;Yt:Z:RU 60 27 GATCCTGTTTTCTGTCTCTCTCTCTCTCTCTGTGTGTATGTGTGTGTGTTTTCCCGAGAAATTACCAATTAGTAAGATTTTCAGAATTTAGTTTGGTATTTATTTGTTTTTGTTTTTTATTTGTAATGTAACCGGAATAAGGTTCTTGCCT CTAAGATGCATCAATTGCTCTCAACCCACCTGGATCACTCTTTTCCCACCCAAGAACTTTTAAAAACTAAGGCCCATCTTTTCTTCTGTGAAACAGGGGTAAGCACTGCTCTAGGCCCTCCTCGTCTCCACTTGCAAGCCTCTCCAATAAA +A00126:224:H7HMWDSX3:3:1104:19804:22701 Test_seq 167974 Test_seq 168041 + - UU A00126:224:H7HMWDSX3:3:1104:19804:22701161Test_seq1679741268M=16797468GGAAGTGCATTGCATGTGTAGATGACTTTTGGTAGAATAGATATTTTTACTATGTTAAGTCTTCCTATFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:6MD:Z:8G2A0T4A6G16C26MC:Z:68MAS:i:40XS:i:33XA:Z:Test_seq,-212997,43M25S,2;Yt:Z:UU A00126:224:H7HMWDSX3:3:1104:19804:2270181Test_seq1679746068M=167974-68GGAAGTGCGTTATATGTATAAATGGCTTTTGGTAGAATAGACATTTTTACTATGTTAAGTCTTCCTAT:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,F:FFFFFFNM:i:1MD:Z:20G47MC:Z:68MAS:i:63XS:i:42Yt:Z:UU 12 60 GGAAGTGCATTGCATGTGTAGATGACTTTTGGTAGAATAGATATTTTTACTATGTTAAGTCTTCCTAT GGAAGTGCGTTATATGTATAAATGGCTTTTGGTAGAATAGACATTTTTACTATGTTAAGTCTTCCTAT +A00126:224:H7HMWDSX3:3:1104:28718:23547 Test_seq 15213 Test_seq 333499 - + UU A00126:224:H7HMWDSX3:3:1104:28718:23547145Test_seq15135679M=333499318287TCCACACAGTCAAATGCCTTTGCATAGTCAATAAAACACAGGTAAACATCTTTTTGGTATTTTCTGCTTTCAGCCAAGAFFFFFFFFFF,,FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:4MD:Z:9G40C2C22G2MC:Z:79MAS:i:61XS:i:56XA:Z:Test_seq,+383985,79M,5;Test_seq,-184559,79M,5;Test_seq,-395912,79M,5;Test_seq,-47050,79M,6;Test_seq,-333499,79M,6;Yt:Z:UU A00126:224:H7HMWDSX3:3:1104:28718:2354797Test_seq3334991179M=15135-318287TCCACACAGTTGAATGCCTTTGCATAGTCAATAAAACACAGGTAAACATCCTTCTGGTATTCTCTGCTTTCAGCCAAGAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:76G2MC:Z:79MAS:i:76XS:i:71XA:Z:Test_seq,-383985,79M,2;Yt:Z:UU 6 11 TCCACACAGTCAAATGCCTTTGCATAGTCAATAAAACACAGGTAAACATCTTTTTGGTATTTTCTGCTTTCAGCCAAGA TCCACACAGTTGAATGCCTTTGCATAGTCAATAAAACACAGGTAAACATCCTTCTGGTATTCTCTGCTTTCAGCCAAGA +A00126:224:H7HMWDSX3:3:1104:21025:24878 ! 0 Test_seq 167670 - - NR A00126:224:H7HMWDSX3:3:1104:21025:24878113Test_seq2135897130M21S=167520-46049ACCAGATAACTGGGAGCTCCTAAAAATCAAACACCTATGCTCATCTAAAGACTTCACCAAAAGAGTAAAAAGACCACCTACAGACTGGGAAAGAATATCCAGCTATGACATCTCCGACCAGCGCCTGATCGATCACCTTTCCTGGTAATAGFFFFFFFFFFFFFF:FFFFF:FFF:FFFFFFF,F:FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFNM:i:3MD:Z:80T15T1T31MC:Z:151MAS:i:115XS:i:110XA:Z:Test_seq,+167316,21S130M,4;Test_seq,+400737,21S123M7S,5;Yt:Z:NR A00126:224:H7HMWDSX3:3:1104:21025:24878177Test_seq16752054151M=21358946049TTTTTCTTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGAFFFFFF,:FFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:130M21SAS:i:151XS:i:126XA:Z:Test_seq,+213368,92M2I23M2I32M,5;Yt:Z:NR 0 54 TTTTTCTTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGA +A00126:224:H7HMWDSX3:3:1104:21576:26490 ! 0 Test_seq 171866 - - NR A00126:224:H7HMWDSX3:3:1104:21576:2649097Test_seq1714536024S127M=171716414GATCTAACTGGAAGCAAATAGATCAGTCCCACATAATTCTTAGGGTTTCTTCATTTTTTTTAATTCTTTTATCTGATTTTTTTTCAGCTATGTTAGTGTTGATTCCCTGGTCCTCCAGATGTCCCAGTCTGCATTCTAATTGCTCGAGTCTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:F,:FFFFFFFFFFFFFFFFFFFF,,FFF:F:F:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF,FFFFFFFFFFFFNM:i:5MD:Z:10G59G1A22A10A20MC:Z:151MAS:i:102XS:i:38Yt:Z:NR A00126:224:H7HMWDSX3:3:1104:21576:26490145Test_seq17171660151M=171453-414TCTTCAACAGTTTTATCAGTGTGTTCCTTGGCTTTTTCTGCAGTTATCCTAATTTCATTTGTGATATCTTTAAGCATTCTGTAAATTAGTTTTTTATATTCTGTATCTGATAATTCCAGGATTGTATCTTCATTTGGGAAAGATTTTGATTFFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:1T149MC:Z:24S127MAS:i:149XS:i:30Yt:Z:NR 0 60 TCTTCAACAGTTTTATCAGTGTGTTCCTTGGCTTTTTCTGCAGTTATCCTAATTTCATTTGTGATATCTTTAAGCATTCTGTAAATTAGTTTTTTATATTCTGTATCTGATAATTCCAGGATTGTATCTTCATTTGGGAAAGATTTTGATT +A00126:224:H7HMWDSX3:3:1104:20826:26913 ! 0 Test_seq 171866 - - NR A00126:224:H7HMWDSX3:3:1104:20826:2691397Test_seq1714536024S127M=171716414GATCTAACTGGAAGCAAATAGATCAGTCCCACATAATTCTTAGGGTTTCTTCATTTTTTTTAATTCTTTTATCTGATTTTTTTTCAGCTATGTTAGTGTTGATTCCCTGGTCCTCCAGATGTCCCAGTCTGCATTCTAATTGCTCGAGTCTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:5MD:Z:10G59G1A22A10A20MC:Z:151MAS:i:102XS:i:38Yt:Z:NR A00126:224:H7HMWDSX3:3:1104:20826:26913145Test_seq17171660151M=171453-414TCTTCAACAGTTTTATCAGTGTGTTCCTTGGCTTTTTCTGCAGTTATCCTAATTTCATTTGTGATATCTTTAAGCATTCTGTAAATTAGTTTTTTATATTCTGTATCTGATAATTCCAGGATTGTATCTTCATTTGGGAAAGATTTTGATTFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFF:FFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFNM:i:1MD:Z:1T149MC:Z:24S127MAS:i:149XS:i:30Yt:Z:NR 0 60 TCTTCAACAGTTTTATCAGTGTGTTCCTTGGCTTTTTCTGCAGTTATCCTAATTTCATTTGTGATATCTTTAAGCATTCTGTAAATTAGTTTTTTATATTCTGTATCTGATAATTCCAGGATTGTATCTTCATTTGGGAAAGATTTTGATT +A00126:224:H7HMWDSX3:3:1104:9453:27070 Test_seq 169139 Test_seq 169447 + - UU A00126:224:H7HMWDSX3:3:1104:9453:2707097Test_seq16913960151M=169297309GTTTAGGTAAGTAGTATTGTTCTAAGAATTTATCCATTTCTTCTAGGTTTTCAAATTTGTTAGAGTACAATTTTATGTAGTAATCTGATATGATTCTTTTGATTTCATTTGGTTCTGTTGTGATGTGGTCCTTCTCGTTTCTTATTCGGGTF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF,FFFFFFFF:FFFFF:FFF,FFFFFFFFFF,FFFF:F:FF:FFFFF,:FF,FFFFFFF:F:FF,FFFFF:FF:::F:FFFFF:FFFFF,F,FFF::F:FF,F,,FF,:F,FFFFNM:i:11MD:Z:9G5G2T3G7C31C12C12A11A11C16T21MC:Z:151MAS:i:96XS:i:58Yt:Z:UU A00126:224:H7HMWDSX3:3:1104:9453:27070145Test_seq16929760151M=169139-309TTCCTTTCCTGTTTTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTT,:FFF,FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFF::::FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFF:FFFFFF:FFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:101Yt:Z:UU 60 60 GTTTAGGTAAGTAGTATTGTTCTAAGAATTTATCCATTTCTTCTAGGTTTTCAAATTTGTTAGAGTACAATTTTATGTAGTAATCTGATATGATTCTTTTGATTTCATTTGGTTCTGTTGTGATGTGGTCCTTCTCGTTTCTTATTCGGGT TTCCTTTCCTGTTTTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTT +A00126:224:H7HMWDSX3:3:1104:31087:28087 ! 0 Test_seq 167675 - - NR A00126:224:H7HMWDSX3:3:1104:31087:28087177Test_seq21359032129M22S=167525-46044CCAGATAACTGGGAGCTCCTAAAAATGAAACACCTATGCTCATCTAAAGACTTCACCAAAAGAGTAAAAAGACCACCTACAGACTGGGAAAGAATTTTCAGCTATGACATCTCCGACCAGCGCCTGATCGATCCTTGTACTGGTCAGTGTGFF,FF:F:FFFFFFFFFFFFFF:,:FFF,FF:FFF,,FFFFFFFFF:FFFFFFF:FFF,,,FFF,FFF:FFFFFFFFF,F:FFF:FFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFF:F:FFFFFFF,,FF,F,FFFFFFFFFFNM:i:2MD:Z:26C52T49MC:Z:151MAS:i:119XS:i:104XA:Z:Test_seq,+167316,22S129M,5;Test_seq,+400737,22S123M6S,4;Yt:Z:NR A00126:224:H7HMWDSX3:3:1104:31087:28087113Test_seq16752552151M=21359046044CTTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTCTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATCFFFFF:FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFNM:i:1MD:Z:65G85MC:Z:129M22SAS:i:146XS:i:121XA:Z:Test_seq,+213363,97M2I23M2I27M,6;Yt:Z:NR 0 52 CTTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTCTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATC +A00126:224:H7HMWDSX3:3:1104:7699:31798 ! 0 Test_seq 170548 - - NR A00126:224:H7HMWDSX3:3:1104:7699:3179897Test_seq1701446025S126M=170398405TTTTGTGAACCATTATCCCATGATCGATCAATTGTCCCTTTTATCATTATATAGTGTCCTTCTTTATCCTTTGTGGCGGATTTAAGTCTAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCCTCTTCTTTTTTGCTTATTATTTGCTTFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF::F,:F,F:F,FFF:FFFFFFF:FFFFFF:::F:NM:i:1MD:Z:52A73MC:Z:151MAS:i:121XS:i:49Yt:Z:NR A00126:224:H7HMWDSX3:3:1104:7699:31798145Test_seq17039860151M=170144-405GGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTTTTCATGTGTGTTGTTGGCCATTTCATTTTTCCACATACTTTTTTGTGCTGAGACGTTTTTCTTAGTAGATTGTGAGATFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:92T58MC:Z:25S126MAS:i:146XS:i:0Yt:Z:NR 0 60 GGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTTTTCATGTGTGTTGTTGGCCATTTCATTTTTCCACATACTTTTTTGTGCTGAGACGTTTTTCTTAGTAGATTGTGAGAT +A00126:224:H7HMWDSX3:3:1104:22869:31923 Test_seq 14083 Test_seq 171253 + + RU A00126:224:H7HMWDSX3:3:1104:22869:31923129Test_seq1408360151M=171253157171TGAGCAATATTTTCCTCTCCCCCTCCCTCCTGCCACTGGTTACCACCTATAAAGTTTGGTGTCTATATATTTGCCTTTTCTTTTTTATTGTGCTTTATGTAAAAGTTTACAAACCAAGTCTATCTCTCATACAAAAATTTATATACATCTTFFFFF,FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFF:F:FFFF:,FFFFFFFFFFFFFFFFF:FFFFFF:FF:FFFFF:F,NM:i:0MD:Z:151MC:Z:36M115SAS:i:151XS:i:0Yt:Z:RU A00126:224:H7HMWDSX3:3:1104:22869:3192365Test_seq171253936M115S=14083-157171GATGATAATATGTCTTGGTGATTTCCTTTTGGGATCGATCAAGTAGACACATGAGACTATGTTAGCATCTCCTGTCTGGAGGGGAGATGAGAGAGCAGAGGGGTCAGAACCTGGTGGAACGGACATGAAAATAGAGAGTGGAGGGAAGGAGFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFNM:i:3MD:Z:20T3T5T5MC:Z:151MAS:i:21XS:i:0SA:Z:Test_seq,14276,-,115M36S,60,0;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1104:22869:319232129Test_seq1427660115M36H=14083-308CTCCTTCCCTCCACTCTCTATTTTCATGTCCGTTCCACCAGGTTCTGACCCCTCTGCTCTCTCATCTCCCCTCCAGACAGGAGATGCTAACATAGTCTCATGTGTCTACTTGATCFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFF:FFFFFFFFFFNM:i:0MD:Z:115MC:Z:151MAS:i:115XS:i:42SA:Z:Test_seq,171253,+,36M115S,9,3;Yt:Z:RU 60 9 TGAGCAATATTTTCCTCTCCCCCTCCCTCCTGCCACTGGTTACCACCTATAAAGTTTGGTGTCTATATATTTGCCTTTTCTTTTTTATTGTGCTTTATGTAAAAGTTTACAAACCAAGTCTATCTCTCATACAAAAATTTATATACATCTT GATGATAATATGTCTTGGTGATTTCCTTTTGGGATCGATCAAGTAGACACATGAGACTATGTTAGCATCTCCTGTCTGGAGGGGAGATGAGAGAGCAGAGGGGTCAGAACCTGGTGGAACGGACATGAAAATAGAGAGTGGAGGGAAGGAG +A00126:224:H7HMWDSX3:3:1104:24505:33880 ! 0 Test_seq 168723 - - NR A00126:224:H7HMWDSX3:3:1104:24505:3388097Test_seq1683476029S122M=168573377CCTTGAGGATGTCCCCTGGGGGAGATTGATCATGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCTTGCCAATCTGGATACGCTTTATTTCTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGCAGTFFFFFFFFFFF:FFFF:FFFF:FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:3MD:Z:18A35C64G2MC:Z:151MAS:i:109XS:i:59Yt:Z:NR A00126:224:H7HMWDSX3:3:1104:24505:33880145Test_seq16857360151M=168347-377TATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGATTACATTAATGFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:143T7MC:Z:29S122MAS:i:146XS:i:71Yt:Z:NR 0 60 TATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGATTACATTAATG +A00126:224:H7HMWDSX3:3:1104:25147:34115 Test_seq 169296 Test_seq 169778 + - UU A00126:224:H7HMWDSX3:3:1104:25147:34115161Test_seq16929660151M=169628483TTTCCTTTCCTGTTTTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACTAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFF:FFFF,:FFFFF:FFFFFFFFFFFFFNM:i:1MD:Z:70C80MC:Z:151MAS:i:146XS:i:96Yt:Z:UU A00126:224:H7HMWDSX3:3:1104:25147:3411581Test_seq16962860151M=169296-483CGTTGCTTTCTAAGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTATTGTTCATTTTCCAAGTATTTGATTTCTTTTCCCTAGTTTTTCTGTTATTGATTTCTAGCTTCATTGCTTTGTGGTCTGAFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFNM:i:4MD:Z:0T34G83C19C11MC:Z:151MAS:i:135XS:i:67Yt:Z:UU 60 60 TTTCCTTTCCTGTTTTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACTAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGT CGTTGCTTTCTAAGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTATTGTTCATTTTCCAAGTATTTGATTTCTTTTCCCTAGTTTTTCTGTTATTGATTTCTAGCTTCATTGCTTTGTGGTCTGA +A00126:224:H7HMWDSX3:3:1104:10004:34538 Test_seq 213885 Test_seq 214212 + - UU A00126:224:H7HMWDSX3:3:1104:10004:34538161Test_seq2138852714S82M1D55M=214062328ACGTGGCCGTGGAGTTAAAACTACGATGAGGTTCCATCTCACTCCAACAAGGCTGGCATTAATCCAAAAAACTCAAAATAATAAATGTTGGAGAGGTGCGGAGAGATTGGAACTCTTATACACTGCTGGTAGGAATGGAAAATGGTACAACFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFNM:i:7MD:Z:6G9A41A23^C10C23G6T13MC:Z:151MAS:i:100XS:i:85XA:Z:Test_seq,-400433,55M1D82M14S,10;Yt:Z:UU A00126:224:H7HMWDSX3:3:1104:10004:3453881Test_seq21406260151M=213885-328AAATAGAACTACCATACAACCCAGAAATCCCACTCCTCGGAATATACCCTAGAGAAATAAGAGCCTTCACACAAACAGATATATGCACACCCATGTTTATTGCAGCTCTGTTTACAATAGCAAAAAGCTGGATGCAACCAAGGTGTCCATCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFNM:i:1MD:Z:132A18MC:Z:14S82M1D55MAS:i:146XS:i:108Yt:Z:UU 27 60 ACGTGGCCGTGGAGTTAAAACTACGATGAGGTTCCATCTCACTCCAACAAGGCTGGCATTAATCCAAAAAACTCAAAATAATAAATGTTGGAGAGGTGCGGAGAGATTGGAACTCTTATACACTGCTGGTAGGAATGGAAAATGGTACAAC AAATAGAACTACCATACAACCCAGAAATCCCACTCCTCGGAATATACCCTAGAGAAATAAGAGCCTTCACACAAACAGATATATGCACACCCATGTTTATTGCAGCTCTGTTTACAATAGCAAAAAGCTGGATGCAACCAAGGTGTCCATC +A00126:224:H7HMWDSX3:3:1104:31819:35524 ! 0 Test_seq 41510 - + MU A00126:224:H7HMWDSX3:3:1104:31819:35524145Test_seq213837050M=41510-172377GATACATGAGAAAATGCTCTCGATCATTAGCCATTAGAGAAATGCAAATTFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:50MC:Z:50MAS:i:50XS:i:50XA:Z:Test_seq,+167148,50M,0;Test_seq,-41510,50M,2;Yt:Z:MU A00126:224:H7HMWDSX3:3:1104:31819:3552497Test_seq41510350M=213837172377GATACATGAGGAAATGCTCACGATCATTAGCCATTAGAGAAATGCAAATTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:2MD:Z:19T29C0MC:Z:50MAS:i:44XS:i:41XA:Z:Test_seq,-49114,4S46M,1;Test_seq,+213837,50M,2;Test_seq,-167148,50M,2;Test_seq,+258764,50M,3;Yt:Z:MU 0 3 GATACATGAGAAAATGCTCTCGATCATTAGCCATTAGAGAAATGCAAATT GATACATGAGGAAATGCTCACGATCATTAGCCATTAGAGAAATGCAAATT +A00126:224:H7HMWDSX3:3:1104:31512:35556 Test_seq 167362 Test_seq 167746 + - UU A00126:224:H7HMWDSX3:3:1104:31512:3555697Test_seq16736221151M=167596385TCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATGCCCTGTATTAGGGCTCCTAGGGTTGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:6A144MC:Z:151MAS:i:146XS:i:136XA:Z:Test_seq,-213528,6S145M,2;Yt:Z:UU A00126:224:H7HMWDSX3:3:1104:31512:35556145Test_seq1675967151M=167362-385GGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATCTTTTCCCCAATTAACTGACACTGGTCCTTTGTCAAATATCAGCTGCTCATACATGGATGGATTTATATCTGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:3MD:Z:80A11G37T20MC:Z:151MAS:i:136XS:i:131XA:Z:Test_seq,+213292,151M,4;Test_seq,+40961,151M,5;Yt:Z:UU 21 7 TCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATGCCCTGTATTAGGGCTCCTAGGGTTG GGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATCTTTTCCCCAATTAACTGACACTGGTCCTTTGTCAAATATCAGCTGCTCATACATGGATGGATTTATATCTG +A00126:224:H7HMWDSX3:3:1104:25093:35556 Test_seq 290555 Test_seq 291016 + - UU A00126:224:H7HMWDSX3:3:1104:25093:3555697Test_seq29055560151M=290866462CCTAGGCAAGATTTTTGCCTTCAGACCTCAGCAGCCAAAATGTTCTCAATGGGAGAGTGCAATTCTAGACACGAGAGCTTGGTTCCAAGAAAGACTGGAAGTCCTCTTGGTACTGGAGACCTCAGGGAAAGGCTGCTTGCAGGTGTCCCATFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFF,FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF,F:F:FF:FFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1104:25093:35556145Test_seq29086660151M=290555-462AAACCCAAGAGATTGGCTCATCCATGCACTGTGAAGAATGTGTAATGGCTCCCATGATGCTTCATCTGTTCTGGGATACAACAGCAGTAACGATACTAAAAATAAAACTTTGTTTTTTCCCAGCGTTATTACATTTTACAAAGTTATATTTFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFF:FF:FFFF,F,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU 60 60 CCTAGGCAAGATTTTTGCCTTCAGACCTCAGCAGCCAAAATGTTCTCAATGGGAGAGTGCAATTCTAGACACGAGAGCTTGGTTCCAAGAAAGACTGGAAGTCCTCTTGGTACTGGAGACCTCAGGGAAAGGCTGCTTGCAGGTGTCCCAT AAACCCAAGAGATTGGCTCATCCATGCACTGTGAAGAATGTGTAATGGCTCCCATGATGCTTCATCTGTTCTGGGATACAACAGCAGTAACGATACTAAAAATAAAACTTTGTTTTTTCCCAGCGTTATTACATTTTACAAAGTTATATTT +A00126:224:H7HMWDSX3:3:1104:6126:35744 Test_seq 168347 Test_seq 168593 + - UU A00126:224:H7HMWDSX3:3:1104:6126:35744161Test_seq1683476019S36M1I95M=168443247AAGTCAGTGGGGGGAGTGATCATGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCCTTACCAATCTGGATACCCTTTATTTCTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGFFFFFFFFFFFFFFFFFFFF,F:FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF:FF::FFFFFFFF::FFFFFFFFFF,FFFF::FF:FFFFFFFFFFF:FFFF:F:FFFFFFFFFF:FFFFFF:FFFF:NM:i:3MD:Z:18A21G90MC:Z:151MAS:i:114XS:i:56Yt:Z:UU A00126:224:H7HMWDSX3:3:1104:6126:3574481Test_seq16844360151M=168347-247CAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCF,FFF:F:FFFFFF:FFFFFFF:FFFFFFFFFFFF:FFFFFFFF,FF:FF:F,FFFFFF,FFF:::FF:FFF:::FFFFFFFF:FFFFFF:FFFFFFFFFF:FFFFFFFFFFFFF,FFFFF,FFFFFF:FFFFFFFFFFFFFFFFFFFFF:NM:i:1MD:Z:52T98MC:Z:19S36M1I95MAS:i:146XS:i:91Yt:Z:UU 60 60 AAGTCAGTGGGGGGAGTGATCATGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCCTTACCAATCTGGATACCCTTTATTTCTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGG CAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTC +A00126:224:H7HMWDSX3:3:1104:21314:35994 Test_seq 166983 Test_seq 167333 + - UU A00126:224:H7HMWDSX3:3:1104:21314:3599497Test_seq16698334151M=167183351AAGATAGCGCCAGATGGATTTCCAAAGTGGTTGTACCATTTTACATTCCCACCATCAGTGTATGAGAGTTCCAATCTCTCCGCACCCTCTCCAACATTTATTATTTTGTGTTTTTTGGATTAATGCCAGTCTAGTTGGTGTGAGATGGTATFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:F:::F:,F:FFFFFF:FF:FFFFFF:FFFFFF,F,::FFFFFFFFFFFFF:F:,FFNM:i:7MD:Z:6A38A8G18G7A2G63A2MC:Z:151MAS:i:118XS:i:99XA:Z:Test_seq,-213901,142M9S,9;Yt:Z:UU A00126:224:H7HMWDSX3:3:1104:21314:35994145Test_seq16718360151M=166983-351ATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAATCATGTAGATTTTAGAGATCAGGAGCTGGTCTGAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:60T90MC:Z:151MAS:i:146XS:i:113Yt:Z:UU 34 60 AAGATAGCGCCAGATGGATTTCCAAAGTGGTTGTACCATTTTACATTCCCACCATCAGTGTATGAGAGTTCCAATCTCTCCGCACCCTCTCCAACATTTATTATTTTGTGTTTTTTGGATTAATGCCAGTCTAGTTGGTGTGAGATGGTAT ATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAATCATGTAGATTTTAGAGATCAGGAGCTGGTCTGA +A00126:224:H7HMWDSX3:3:1105:4218:2722 Test_seq 126403 Test_seq 126873 + - UU A00126:224:H7HMWDSX3:3:1105:4218:272297Test_seq1264036019S132M=126723471CCCACAACTCCTATAGATCAATCAACTCGACAGCAACAGATTTGGTTTTTAGAAACCTACACAGCTGAGCTCTGGATTGGGACAATCAAAATAGCAATTCCCTGGGCCTCAGCTCTCTCCCAGAAAGTAATTAATGCCATGGATATGGCTTFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,F,FFFFFFFFFFFFFFNM:i:0MD:Z:132MC:Z:151MAS:i:132XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1105:4218:2722145Test_seq12672360151M=126403-471GGATCCTCGGGCTCCCAAGGAGGCTGTGTGGCTAGAACGTAAGAAGTCTGTGAACTTAGATGGGAAAAAAAAAGTACATATTTATTTGTGCTGACTTCTAACTGAAAATTAGTATACCCATCAGTAGCGACTGTCAGCTGTGACTTTGTCCFFFFFFFFFFFF:FFF,FFFFFFFFFFFFFF:,FFFFFFFFFFFFFFFFFFFFFFF,F:F,FFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:19S132MAS:i:151XS:i:0Yt:Z:UU 60 60 CCCACAACTCCTATAGATCAATCAACTCGACAGCAACAGATTTGGTTTTTAGAAACCTACACAGCTGAGCTCTGGATTGGGACAATCAAAATAGCAATTCCCTGGGCCTCAGCTCTCTCCCAGAAAGTAATTAATGCCATGGATATGGCTT GGATCCTCGGGCTCCCAAGGAGGCTGTGTGGCTAGAACGTAAGAAGTCTGTGAACTTAGATGGGAAAAAAAAAGTACATATTTATTTGTGCTGACTTCTAACTGAAAATTAGTATACCCATCAGTAGCGACTGTCAGCTGTGACTTTGTCC +A00126:224:H7HMWDSX3:3:1105:25699:3129 Test_seq 171489 Test_seq 171934 + - UU A00126:224:H7HMWDSX3:3:1105:25699:3129161Test_seq1714896016M1D135M=171784446TAATTCTTTTATCTGATTTTTTTCAGCTATGTTGGTGGTGTTTTCCTGGTCCTCCAGAAGTCCCAGTCTACATTCTAATTGCTCGAGTCTGCTCCTCTGACTTCCTATTGCGTTGTCAAATTCTGTAATTTTATTGTTAATCTTTTGGATTFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFNM:i:8MD:Z:16^T19A1T2A2C49T9T13T33MC:Z:151MAS:i:109XS:i:32Yt:Z:UU A00126:224:H7HMWDSX3:3:1105:25699:312981Test_seq17178460151M=171489-446TGTAAGCATTCTGTAAATTAGTTTTTTATATTCTGTATCTGATAATTCCAGGATTGTATCTTCATTTGGGAAAGATTTTGATTCTTTTGTTTGGGGGGTTGGAGAAGCTGTCATGGTCTGTTTCTTTATGTGGTTTGATATGGACTGCTGTFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:1T149MC:Z:16M1D135MAS:i:149XS:i:21Yt:Z:UU 60 60 TAATTCTTTTATCTGATTTTTTTCAGCTATGTTGGTGGTGTTTTCCTGGTCCTCCAGAAGTCCCAGTCTACATTCTAATTGCTCGAGTCTGCTCCTCTGACTTCCTATTGCGTTGTCAAATTCTGTAATTTTATTGTTAATCTTTTGGATT TGTAAGCATTCTGTAAATTAGTTTTTTATATTCTGTATCTGATAATTCCAGGATTGTATCTTCATTTGGGAAAGATTTTGATTCTTTTGTTTGGGGGGTTGGAGAAGCTGTCATGGTCTGTTTCTTTATGTGGTTTGATATGGACTGCTGT +A00126:224:H7HMWDSX3:3:1105:6090:3865 Test_seq 213577 Test_seq 213928 + - UU A00126:224:H7HMWDSX3:3:1105:6090:386597Test_seq21357718151M=213778352TGATGAAGAGAAACCAGATAACTGGGAGCTCCTAAAAATCAAACACCTATGCTCATCTAAAGACTTCACCAAAAGAGTAAAAAGACCACCTACAGACTGGGAAAGAATTTTCAGCTATGACATCTCCGACTAGCACCTGATCTCTAAAATCFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF,FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFNM:i:4MD:Z:0C91T37C3G16MC:Z:151MAS:i:135XS:i:126XA:Z:Test_seq,-167307,151M,5;Test_seq,-400728,132M19S,3;Yt:Z:UU A00126:224:H7HMWDSX3:3:1105:6090:3865145Test_seq21377860151M=213577-352GAAGTGGGCAAAGGATATGAACACGCACTTCACTAAAGAAGATATTCAGGCAGCTAACAGATACATGAGAAAATGCTCTCGATCATTAGCCATTAGAGAAATGCAAATTAAAAGTACGATGAGATTCCATCTCACTCCAACAAGGCTGGCAFFFFF::FF,FFFFFFFFFFF:FF,,:,F:,FFFFFFFFF:FFFF,FFF:FFFFFFFFFFFFFFFFFF,FFFFF:FFFF:FFFFFFFFF,FFFFFFFF:FFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFF:FF:FFFFFFFF::FFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:106Yt:Z:UU 18 60 TGATGAAGAGAAACCAGATAACTGGGAGCTCCTAAAAATCAAACACCTATGCTCATCTAAAGACTTCACCAAAAGAGTAAAAAGACCACCTACAGACTGGGAAAGAATTTTCAGCTATGACATCTCCGACTAGCACCTGATCTCTAAAATC GAAGTGGGCAAAGGATATGAACACGCACTTCACTAAAGAAGATATTCAGGCAGCTAACAGATACATGAGAAAATGCTCTCGATCATTAGCCATTAGAGAAATGCAAATTAAAAGTACGATGAGATTCCATCTCACTCCAACAAGGCTGGCA +A00126:224:H7HMWDSX3:3:1105:5593:4758 Test_seq 310384 Test_seq 310718 + - UU A00126:224:H7HMWDSX3:3:1105:5593:475897Test_seq31038460151M=310568335GATCTACAGCTCATCAGGGAGAGTAGGAGTTAGGGACGGGGCTTAGGAAGCCAGGGACAAGGTGAAGTTCTGGCTGTGCATGAAATTGTCAAAGAGGAACATGAAAAGAGAAGGACAAAGGACTAAAGCTCAAGCAGAAAAATAAAGTGGTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1105:5593:4758145Test_seq31056860151M=310384-335CTAAATATCTACAATACCTGCTTTATTGTTACAGTTGCGTACCATCTAGTTGTCTCCAACTCATAGCAACCCTATAGGACAGCAGAACTGCCCCACAGGGTTTTCTAGGCTGAAATCTTTATGGGAGCAGATCACTAGGTCTTTCTCCTGAFFFFFF,:FFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:28Yt:Z:UU 60 60 GATCTACAGCTCATCAGGGAGAGTAGGAGTTAGGGACGGGGCTTAGGAAGCCAGGGACAAGGTGAAGTTCTGGCTGTGCATGAAATTGTCAAAGAGGAACATGAAAAGAGAAGGACAAAGGACTAAAGCTCAAGCAGAAAAATAAAGTGGT CTAAATATCTACAATACCTGCTTTATTGTTACAGTTGCGTACCATCTAGTTGTCTCCAACTCATAGCAACCCTATAGGACAGCAGAACTGCCCCACAGGGTTTTCTAGGCTGAAATCTTTATGGGAGCAGATCACTAGGTCTTTCTCCTGA +A00126:224:H7HMWDSX3:3:1105:8359:6292 Test_seq 356837 Test_seq 357268 + - UU A00126:224:H7HMWDSX3:3:1105:8359:6292161Test_seq35683760151M=357118432ATAATGTCATGTAGACTTTATCATCTCAAATGACACCAATATTTTATACTTTTAAGTCAAAAGTTAAACTTTTAAATGCCCATTTTGACTTGAGATCTTCAATCCTTTAATTCAATACTGTTAGAATAAATGTTTAAAATGCAGAATTTAGFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1105:8359:629281Test_seq35711860151M=356837-432AAAACCACAAAATAATAAATGTTAGAGAGGTTGTGGAGAGACTGGAATACTTAGGGGCAGCTGGTGGGAATGTAAAATGGTACAGTCACTTTGGAAGTTGATTTGGCACTTATTTAAAAAGCTAGGAATAGAACTACCATAATATCCAGGAFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:31Yt:Z:UU 60 60 ATAATGTCATGTAGACTTTATCATCTCAAATGACACCAATATTTTATACTTTTAAGTCAAAAGTTAAACTTTTAAATGCCCATTTTGACTTGAGATCTTCAATCCTTTAATTCAATACTGTTAGAATAAATGTTTAAAATGCAGAATTTAG AAAACCACAAAATAATAAATGTTAGAGAGGTTGTGGAGAGACTGGAATACTTAGGGGCAGCTGGTGGGAATGTAAAATGGTACAGTCACTTTGGAAGTTGATTTGGCACTTATTTAAAAAGCTAGGAATAGAACTACCATAATATCCAGGA +A00126:224:H7HMWDSX3:3:1105:11776:6480 Test_seq 168519 Test_seq 168902 + - UU A00126:224:H7HMWDSX3:3:1105:11776:6480161Test_seq1685196049M1D102M=168752384GTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTATTATGTTGAGGAATTTTCCTTCTATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF,FFFFFFFFFFFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFFFFF:FFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFFNM:i:2MD:Z:49^T25A76MC:Z:151MAS:i:139XS:i:79Yt:Z:UU A00126:224:H7HMWDSX3:3:1105:11776:648081Test_seq16875260151M=168519-384TACCTGGTATAAATCCCACTTGATCATGGTGAATTATTTTTTTGATGTGTTGTTGGATTCTATTGGCTAGAATTTTGTTGAGGATTTTTGCATCTATGTTCATGAGGGATATAGGTCTAAAATTTTCTTTTTTTGTAATGTCTTTACCTGGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF,:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:26G124MC:Z:49M1D102MAS:i:146XS:i:83Yt:Z:UU 60 60 GTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTATTATGTTGAGGAATTTTCCTTCTATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATA TACCTGGTATAAATCCCACTTGATCATGGTGAATTATTTTTTTGATGTGTTGTTGGATTCTATTGGCTAGAATTTTGTTGAGGATTTTTGCATCTATGTTCATGAGGGATATAGGTCTAAAATTTTCTTTTTTTGTAATGTCTTTACCTGG +A00126:224:H7HMWDSX3:3:1105:7862:8343 Test_seq 258022 Test_seq 258423 + - UU A00126:224:H7HMWDSX3:3:1105:7862:8343161Test_seq2580226020S131M=258273402ATGCCTGCACAGCAGTCTCTCAGATCACCAACTTCATATGGAAAGGGAAGCATCTCTGGATAAGTAAATCATTACTGAAAAAGAAGAACAAAGTGAAAGGCCTCACACTGTCTGATTTTAGAACCTATTATAATGCCACAGTAGTCAAAACFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFNM:i:0MD:Z:131MC:Z:151MAS:i:131XS:i:32Yt:Z:UU A00126:224:H7HMWDSX3:3:1105:7862:834381Test_seq25827360151M=258022-402GGGAAAAGACAGTCTCTTTAACAAATGGTGCTGGCACAACTGGATATCCATCAGCAAAAAAATGCAACAAGACTCATACTTCACACCATGCACAAAAACGAACTCAAAATGAATCAAAGACCTAAATATAAAATCTAAAACAATAAAGATCF,:FFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFF:::FF:FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFNM:i:0MD:Z:151MC:Z:20S131MAS:i:151XS:i:66Yt:Z:UU 60 60 ATGCCTGCACAGCAGTCTCTCAGATCACCAACTTCATATGGAAAGGGAAGCATCTCTGGATAAGTAAATCATTACTGAAAAAGAAGAACAAAGTGAAAGGCCTCACACTGTCTGATTTTAGAACCTATTATAATGCCACAGTAGTCAAAAC GGGAAAAGACAGTCTCTTTAACAAATGGTGCTGGCACAACTGGATATCCATCAGCAAAAAAATGCAACAAGACTCATACTTCACACCATGCACAAAAACGAACTCAAAATGAATCAAAGACCTAAATATAAAATCTAAAACAATAAAGATC +A00126:224:H7HMWDSX3:3:1105:24858:9220 Test_seq 56335 Test_seq 56759 + - UU A00126:224:H7HMWDSX3:3:1105:24858:922097Test_seq5633560151M=56613425CAAAGAAGAAGTTGTTGGTCTTGCAAAATTCTATCATGCAATCACTGGTGTCATTTCTATCACCAAGGCCGTATTTTCCAACTACCAATCCTTTTTCTTTGTTTCCAACTTTCAAATTCCTATCACCAGTAATTATCAACGCATCATGACTFFF,FFFFFF:FFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFF:FFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFF:FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:116M4I31MAS:i:151XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1105:24858:9220145Test_seq5661360116M4I31M=56335-425TATCACTGACAGCATTTTACTTCAGGATAGATCTTGAAATGTTCTTTTTGATGATGAATGCAATGCCATTCCTCTTCAAGTTGTCATTCCCAGCATAGTAGACCACATGATTGTCTGATCGATCCAAAATGGCCAATATCAGTCCATTGCAFFFFFFFFFFFFF:FFFFFFFFFFFF,FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFNM:i:4MD:Z:147MC:Z:151MAS:i:137XS:i:84Yt:Z:UU 60 60 CAAAGAAGAAGTTGTTGGTCTTGCAAAATTCTATCATGCAATCACTGGTGTCATTTCTATCACCAAGGCCGTATTTTCCAACTACCAATCCTTTTTCTTTGTTTCCAACTTTCAAATTCCTATCACCAGTAATTATCAACGCATCATGACT TATCACTGACAGCATTTTACTTCAGGATAGATCTTGAAATGTTCTTTTTGATGATGAATGCAATGCCATTCCTCTTCAAGTTGTCATTCCCAGCATAGTAGACCACATGATTGTCTGATCGATCCAAAATGGCCAATATCAGTCCATTGCA +A00126:224:H7HMWDSX3:3:1105:3079:9267 Test_seq 169966 Test_seq 170357 + - UU A00126:224:H7HMWDSX3:3:1105:3079:926797Test_seq16996660151M=170207392GTGTCTCTATTGAGCTTCTTACTGGATGTCCTGTCCTTCTCCGAAAGTGGTGTGTTGAAGTCTCCTACTATATATGTGGAGGTGTCTATCTCACTTTTCAATTCTGTTAAAATTTGATTTATGTATCTTGCAGCCCTGTCATTAGGTGCGTFFFFFFFFFFFFFFFFFFFFF:FF:FFFF:FFFFFFFFFFFF:FFFFFFFFF::FFFFFFF:F:FFFFF:F:FFFFFFFFF,F,FFFFFFFFFFFFFFFFFFFFF:FFFF,FFFF,FFFFFFFFFFFFFF:FF,F,FFFF::FF,FF:FFFNM:i:7MD:Z:8G53C9A8A10G48C1G7MC:Z:151MAS:i:116XS:i:52Yt:Z:UU A00126:224:H7HMWDSX3:3:1105:3079:9267145Test_seq17020760151M=169966-392TAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCCTCTTCTTTTTTGCTTATTGTTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATC:F,FFFFFFF:FFF,FFFF:FFFFFFFFFF,:FFFFFFFF:F,,:FFFFFF:FFF,FFFFFFF,FFFFFFFF,F:FFFFF:FFFFFFF:FF:FFFFFF:F:FFFFFFFFFFF:FFF,,FFFFFFF,FF,FFFF,FFF:FFFFFFFF,FFF,NM:i:1MD:Z:55A95MC:Z:151MAS:i:146XS:i:88Yt:Z:UU 60 60 GTGTCTCTATTGAGCTTCTTACTGGATGTCCTGTCCTTCTCCGAAAGTGGTGTGTTGAAGTCTCCTACTATATATGTGGAGGTGTCTATCTCACTTTTCAATTCTGTTAAAATTTGATTTATGTATCTTGCAGCCCTGTCATTAGGTGCGT TAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCCTCTTCTTTTTTGCTTATTGTTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATC +A00126:224:H7HMWDSX3:3:1105:24840:9659 Test_seq 170869 Test_seq 171176 + - UU A00126:224:H7HMWDSX3:3:1105:24840:965997Test_seq17086960151M=171041308GAGTTGCGTTGATATCAGGACGTTCTGTTTTGTGACCTTGTATTGTGCTGGTACCTGATATTATTGGTCATCAGGCCAAACAATCTCCTTTAGCATTTCTTGCAGTCTTGGTTTAGTTTTTGCAAATTCTCTAAACTTGTGTTTATCTGTAFFFFFFFFFFFFFFFFFFFFFFF:,FFFFFF:F:,FF:FFFFFFFF,F:FFFFFFFFFFFFFFFF,FFFFFFFF:FFFFF:FF::FFF,:FF:FFFFF,:FFF:F,F,FFFF:FFFFFFFF:FFFFF:FFF:FFF,:F:FFFFFFF,F,FFNM:i:1MD:Z:90A60MC:Z:136M15SAS:i:146XS:i:23Yt:Z:UU A00126:224:H7HMWDSX3:3:1105:24840:9659145Test_seq17104160136M15S=170869-308TATTTCAGAGAGAGTTTTGCTGGATATATGATCCTTGGTTGGCAGTTTTTCTCCTTCAGTGCTCTGTATACGTCATCCCATTCCCTTCTTGCCTGCATGGTGTCTGCTGAGTAGTCTGAACTTATTCTTATTGATTAAAGCACCCCCAGGGFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:4MD:Z:6C43A23G26T34MC:Z:151MAS:i:116XS:i:76Yt:Z:UU 60 60 GAGTTGCGTTGATATCAGGACGTTCTGTTTTGTGACCTTGTATTGTGCTGGTACCTGATATTATTGGTCATCAGGCCAAACAATCTCCTTTAGCATTTCTTGCAGTCTTGGTTTAGTTTTTGCAAATTCTCTAAACTTGTGTTTATCTGTA TATTTCAGAGAGAGTTTTGCTGGATATATGATCCTTGGTTGGCAGTTTTTCTCCTTCAGTGCTCTGTATACGTCATCCCATTCCCTTCTTGCCTGCATGGTGTCTGCTGAGTAGTCTGAACTTATTCTTATTGATTAAAGCACCCCCAGGG +A00126:224:H7HMWDSX3:3:1105:29984:9768 Test_seq 169353 Test_seq 169775 + - UU A00126:224:H7HMWDSX3:3:1105:29984:9768161Test_seq16935360151M=169626423TTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGATTCTTTTGTTGCTCACTTTCTATTTGTTCAAGTTGTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFF:FFFFFFFFFFFF,FFFFFF:FFF:FFFFFFFFFF:,FFFFF,FFFF,FFFFFFFFFFFFFNM:i:1MD:Z:114G36MC:Z:57M1I93MAS:i:146XS:i:101Yt:Z:UU A00126:224:H7HMWDSX3:3:1105:29984:976881Test_seq1696266057M1I93M=169353-423CTCGTTGCTTTCTATGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGTCTTTTTTTCAGGAGGGTATTGTTCATTTTCCAAGTATTTGATTTCTTTTCCCTAGTTTTTCTGTTATTGATTTCTAGCTTCATTGCCTTGTGGCCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFNM:i:6MD:Z:2T11A22G83C26T1MC:Z:151MAS:i:123XS:i:65Yt:Z:UU 60 60 TTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGATTCTTTTGTTGCTCACTTTCTATTTGTTCAAGTTGT CTCGTTGCTTTCTATGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGTCTTTTTTTCAGGAGGGTATTGTTCATTTTCCAAGTATTTGATTTCTTTTCCCTAGTTTTTCTGTTATTGATTTCTAGCTTCATTGCCTTGTGGCC +A00126:224:H7HMWDSX3:3:1105:27724:12399 Test_seq 384489 Test_seq 384540 + - UU A00126:224:H7HMWDSX3:3:1105:27724:1239997Test_seq384489552M=38449952ATGGAGAAAAGATTGAAGTTGTCAAGGATTTCATTTTACTTGGATCCACAATFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:1C50MC:Z:10S42MAS:i:50XS:i:47XA:Z:Test_seq,-333022,52M,1;Test_seq,+204890,3S49M,1;Yt:Z:UU A00126:224:H7HMWDSX3:3:1105:27724:12399145Test_seq384499410S42M=384489-52ATGGAGTGATGATTGAAGTTGTCAAGGATTTCATTTTACTTGGATCCACAATFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:NM:i:0MD:Z:42MC:Z:52MAS:i:42XS:i:40Yt:Z:UU 5 4 ATGGAGAAAAGATTGAAGTTGTCAAGGATTTCATTTTACTTGGATCCACAAT ATGGAGTGATGATTGAAGTTGTCAAGGATTTCATTTTACTTGGATCCACAAT +A00126:224:H7HMWDSX3:3:1105:30861:14951 ! 0 Test_seq 171173 - + NR A00126:224:H7HMWDSX3:3:1105:30861:14951145Test_seq17150260127M24S=171173-456TGATTTTTTTTCAGCTATGTTGGTGTTGTTTCCCTGGTCCTCCAGAAGTCCCAGTCTACATTCTAATTTCTCGAATCTGCTCCTCTGACTTCCTATTGCATTGTCTAATTCTGTAATTTTATTGTTAGATCTTAATTTGTTTTTGTGATTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:7MD:Z:23A4A39G5G6T9T7G27MC:Z:151MAS:i:92XS:i:25Yt:Z:NR A00126:224:H7HMWDSX3:3:1105:30861:1495197Test_seq17117360151M=171502456GATTCTCCTTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATGGGGTTCGATGAGCATCTTGGATAGFFFFFFFFFFF:FFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:NM:i:1MD:Z:8C142MC:Z:127M24SAS:i:146XS:i:36Yt:Z:NR 0 60 GATTCTCCTTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATGGGGTTCGATGAGCATCTTGGATAG +A00126:224:H7HMWDSX3:3:1105:7419:15436 Test_seq 214052 Test_seq 214417 + - UU A00126:224:H7HMWDSX3:3:1105:7419:1543697Test_seq21405260151M=214268366AAAAAGTTAGAAATAGAACTACCATACAACCCAGAAATCCCACTCCTCGGAATATACCCTAGAGAAATAAGAGCCTTCACACAAACAGATATATGCACACCCATGTTTACTGCAGCTCTGTTTACAATAGCAAAAAGCTGGAAGCAACCAAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFNM:i:1MD:Z:109T41MC:Z:117M1I33MAS:i:146XS:i:106Yt:Z:UU A00126:224:H7HMWDSX3:3:1105:7419:15436145Test_seq21426836117M1I33M=214052-366TCGATAAAGAACAGTGACGAATCTGGGAAACATTTCATAACATGGAGGAACCTGGAAGGCATTATGCTGAGCGAAATTAGTCAGAGGCAAAAGGACAAATATTGTATAAGACCACGATTATAAGATCTTGAGAAATAGTATAAACTGAGAAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF,FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:6MD:Z:2A22T45T29C13T34MC:Z:151MAS:i:120XS:i:100XA:Z:Test_seq,+400037,151M,11;Yt:Z:UU 60 36 AAAAAGTTAGAAATAGAACTACCATACAACCCAGAAATCCCACTCCTCGGAATATACCCTAGAGAAATAAGAGCCTTCACACAAACAGATATATGCACACCCATGTTTACTGCAGCTCTGTTTACAATAGCAAAAAGCTGGAAGCAACCAA TCGATAAAGAACAGTGACGAATCTGGGAAACATTTCATAACATGGAGGAACCTGGAAGGCATTATGCTGAGCGAAATTAGTCAGAGGCAAAAGGACAAATATTGTATAAGACCACGATTATAAGATCTTGAGAAATAGTATAAACTGAGAA +A00126:224:H7HMWDSX3:3:1105:23077:15562 ! 0 Test_seq 167509 - - NR A00126:224:H7HMWDSX3:3:1105:23077:15562161Test_seq1671312225S126M=167359379CCTCCACCCTCTTAAAAATATGTTAAATCTCATCGTAGTTTTAATTTGCATTTCTCTAATGGCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFNM:i:2MD:Z:13C98T13MC:Z:151MAS:i:116XS:i:106XA:Z:Test_seq,-213778,126M25S,4;Yt:Z:NR A00126:224:H7HMWDSX3:3:1105:23077:1556281Test_seq16735917151M=167131-379CAGTCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATGCCCTGTATTAGGGCTCCTAGGGFFFFFFFFFFFFFFFFFFFFF:FFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFF:FF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFNM:i:1MD:Z:9A141MC:Z:25S126MAS:i:146XS:i:138XA:Z:Test_seq,+213528,3S148M,2;Yt:Z:NR 0 17 CAGTCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATGCCCTGTATTAGGGCTCCTAGGG +A00126:224:H7HMWDSX3:3:1105:27923:18443 ! 0 Test_seq 168451 - + NR A00126:224:H7HMWDSX3:3:1105:27923:1844381Test_seq16868960122M29S=168451-360CTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTAATCTACAGTATGATAAAAAAAAAAGTTA:FFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFF,FFFFFFFFFFFFFF:FFFFFFFFFFFFFFF::FFFFF:FFFFFFFFFFFFFFFFFFFFF:FFFFFF,FFFFFFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:27T94MC:Z:151MAS:i:117XS:i:54Yt:Z:NR A00126:224:H7HMWDSX3:3:1105:27923:18443161Test_seq16845160151M=168689360ATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTA:FFFF,FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF,:FFF:FFFF,FFFFFFFF:FFFFFF:FF,FFFNM:i:1MD:Z:44T106MC:Z:122M29SAS:i:146XS:i:93Yt:Z:NR 0 60 ATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTA +A00126:224:H7HMWDSX3:3:1105:8820:20337 Test_seq 171233 Test_seq 171572 + - UU A00126:224:H7HMWDSX3:3:1105:8820:20337161Test_seq17123360151M=171441340TCTTTGGTTTTGGCAAGTTTGATGATAATATATCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATGGGGTTCGATGAGCATCTTGGATAGATATCCTTTCGTCTTTCATGATGTCAGGGAAGTTTTGTGTCAGGAGTTCTTCAACTATTTFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFF,FFFFFFFFF:FFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFF:FFFFFFFFF,FFFFFFFFNM:i:1MD:Z:31G119MC:Z:132M19SAS:i:146XS:i:36Yt:Z:UU A00126:224:H7HMWDSX3:3:1105:8820:2033781Test_seq17144160132M19S=171233-340CCTTCTTGATAGAGTCCCACATGATTCTTAGGGTTTCTTCATTTTTTTTAATTCTTTTATCTGATTTTTTTTCGGCTATGTTGGTGTTGATTTCCTGGTCCTCCAGAAGTCCCAGTCTACATTCTAATTGCTTGAGTTAATTTTTCTACTAFFFFFF:FFFFFF:FFFFFF:FFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFF:FFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:3MD:Z:73A10A7C39MC:Z:151MAS:i:117XS:i:43Yt:Z:UU 60 60 TCTTTGGTTTTGGCAAGTTTGATGATAATATATCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATGGGGTTCGATGAGCATCTTGGATAGATATCCTTTCGTCTTTCATGATGTCAGGGAAGTTTTGTGTCAGGAGTTCTTCAACTATTT CCTTCTTGATAGAGTCCCACATGATTCTTAGGGTTTCTTCATTTTTTTTAATTCTTTTATCTGATTTTTTTTCGGCTATGTTGGTGTTGATTTCCTGGTCCTCCAGAAGTCCCAGTCTACATTCTAATTGCTTGAGTTAATTTTTCTACTA +A00126:224:H7HMWDSX3:3:1105:10276:21010 ! 0 Test_seq 171288 - - MR A00126:224:H7HMWDSX3:3:1105:10276:210102145Test_seq1710706019H132M=171138219GATCCTTGGTTGGCAGATTTTCTCCTTCAGTGCTCTGTATACGTCATCCCATTCCCTTCTTGCCTGCATGGTTTCTGCTGAGTAGTCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,,,:,,FFFFFFNM:i:3MD:Z:16T4A23G86MC:Z:151MAS:i:117XS:i:74SA:Z:Test_seq,51877,-,132S19M,0,0;Yt:Z:MRNEXT_SAMA00126:224:H7HMWDSX3:3:1105:10276:21010113Test_seq518770132S19M=171138119394AAAAGAAAGGTTTCCTTCAAGGGAGAATCAATAAGAATAAGTTCAGACTACTCAGCAGAAACCATGCAGGCAAGAAGGGAATGGGATGACGTATACAGAGCACTGAAGGAGAAAATCTGCCAACCAAGGATCGATCAAGTTGGTTGATTGTFFFFFF,,:,,,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:19MC:Z:151MAS:i:19XS:i:0SA:Z:Test_seq,171070,+,19S132M,60,3;Yt:Z:MR A00126:224:H7HMWDSX3:3:1105:10276:21010177Test_seq17113860151M=51877-119394TGGTTTCTGCTGAGTAGTCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTGGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:105T45MC:Z:132S19MAS:i:146XS:i:0Yt:Z:MR 0 60 AAAAGAAAGGTTTCCTTCAAGGGAGAATCAATAAGAATAAGTTCAGACTACTCAGCAGAAACCATGCAGGCAAGAAGGGAATGGGATGACGTATACAGAGCACTGAAGGAGAAAATCTGCCAACCAAGGATCGATCAAGTTGGTTGATTGT TGGTTTCTGCTGAGTAGTCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTGGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATC +A00126:224:H7HMWDSX3:3:1105:18837:21089 Test_seq 165754 Test_seq 166211 + - UU A00126:224:H7HMWDSX3:3:1105:18837:2108997Test_seq16575460151M=166061458AGTCAGCCCTTTCAGTCTCTCGTTTCGTGCCAATTTTGCCGTCTTCCCTCTCTCTCTATCTTCCCATCCCCCCTCCAGTCAAGAGTTGCCAACACACTCTCCAGTGTCCACCTAATTTAATTAGCTCACTCTTCATCAGCATCTCTCTCCCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFNM:i:3MD:Z:40A5T75G28MC:Z:151MAS:i:136XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1105:18837:21089145Test_seq16606160151M=165754-458TATCCCATTGGTCTCCTGCTCCCTCAGGAGTTGTCTGTTGTGCTCCCTGACAGGGCAGACATCGATTGTGGCCGGGCACCAATTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCTTFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:82C68MC:Z:151MAS:i:146XS:i:75Yt:Z:UU 60 60 AGTCAGCCCTTTCAGTCTCTCGTTTCGTGCCAATTTTGCCGTCTTCCCTCTCTCTCTATCTTCCCATCCCCCCTCCAGTCAAGAGTTGCCAACACACTCTCCAGTGTCCACCTAATTTAATTAGCTCACTCTTCATCAGCATCTCTCTCCC TATCCCATTGGTCTCCTGCTCCCTCAGGAGTTGTCTGTTGTGCTCCCTGACAGGGCAGACATCGATTGTGGCCGGGCACCAATTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCTT +A00126:224:H7HMWDSX3:3:1105:16640:21167 ! 0 Test_seq 167661 - - NR A00126:224:H7HMWDSX3:3:1105:16640:2116797Test_seq167316722S129M=167511346TGAACCCTGATTGATTTGGATCGATCAGGCGCTGGTCTGAGATGTCATAGCTGAATATTCTTTCCCAATCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCGGGFFFFFFF:FFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFF:FFFFFFFFFFF:FFFFFFFFFFF:FF:FFFFFFF,F:FFFFFFFFFFFFFFFFFFFFFF:FFFFF,FFFF,FFFFFFFF:FFFFFFFFF:FFFFFNM:i:4MD:Z:7A37G6A73T2MC:Z:151MAS:i:111XS:i:106XA:Z:Test_seq,-213590,129M22S,5;Test_seq,+400737,22S123M6S,6;Yt:Z:NR A00126:224:H7HMWDSX3:3:1105:16640:21167145Test_seq16751160151M=167316-346TGATCCTATTTTTTCTTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFF:FFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:22S129MAS:i:151XS:i:122XA:Z:Test_seq,+213377,83M2I23M2I37M4S,5;Yt:Z:NR 0 60 TGATCCTATTTTTTCTTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTG +A00126:224:H7HMWDSX3:3:1105:7835:24173 Test_seq 171202 Test_seq 171530 + - UU A00126:224:H7HMWDSX3:3:1105:7835:2417397Test_seq17120260151M=171381329CTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATGGGGTTCGATGCGCATCTTGGATAGATATCCTTTCGTCTTTCATGATGTCAGGGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:108A42MC:Z:101M1I49MAS:i:146XS:i:76Yt:Z:UU A00126:224:H7HMWDSX3:3:1105:7835:24173145Test_seq17138160101M1I49M=171202-329TTTTCTCTGTGTTTTCTGTCCCCTCTTCCTGTTCTGGGACTCCAATCACTCACAAGTTATCCTTCTTGATAGAGTCCCACATGATTCTTAGGGTTTCTCCATTTTTTTTTAATTCTTTTATCTGATTTTTTTTCAGCTATGTTGGTGTTGAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:8MD:Z:19G3C2C8T15G46T45A5MC:Z:151MAS:i:108XS:i:28Yt:Z:UU 60 60 CTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATGGGGTTCGATGCGCATCTTGGATAGATATCCTTTCGTCTTTCATGATGTCAGGG TTTTCTCTGTGTTTTCTGTCCCCTCTTCCTGTTCTGGGACTCCAATCACTCACAAGTTATCCTTCTTGATAGAGTCCCACATGATTCTTAGGGTTTCTCCATTTTTTTTTAATTCTTTTATCTGATTTTTTTTCAGCTATGTTGGTGTTGA +A00126:224:H7HMWDSX3:3:1105:19759:25692 Test_seq 167505 Test_seq 213736 - - UU A00126:224:H7HMWDSX3:3:1105:19759:25692113Test_seq1673557151M=21358646232TTCCCAGTCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATGCCCTGTATTAGGGCTCCTFFFFFF:F,FFF:FFFFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFNM:i:1MD:Z:13A137MC:Z:151MAS:i:146XS:i:141XA:Z:Test_seq,+213529,151M,2;Yt:Z:UU A00126:224:H7HMWDSX3:3:1105:19759:25692177Test_seq21358619151M=167355-46232GAAACCAGATAACTGGGAGCTCCTAAAAATCAAACACCTATGCTCATCTAAAGACTTCACCAAAAGAGTAAAAAGACCACCTACAAAATGGGAAAAAATTTTCAGCTATGACATCTCTGACCAGCGCCTGATCTCTAAAATCTATATGATTFFFFFFFFFFFFFFFFFFFFFFFFF:FF::FFFF:,FFF,FFFFFF,FFFF,FF:F:FFFFFFFFFF:FFFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:5MD:Z:83T1G1C7G21C33MC:Z:151MAS:i:126XS:i:116XA:Z:Test_seq,+400719,141M10S,5;Test_seq,+167298,151M,8;Yt:Z:UU 7 19 TTCCCAGTCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATGCCCTGTATTAGGGCTCCT GAAACCAGATAACTGGGAGCTCCTAAAAATCAAACACCTATGCTCATCTAAAGACTTCACCAAAAGAGTAAAAAGACCACCTACAAAATGGGAAAAAATTTTCAGCTATGACATCTCTGACCAGCGCCTGATCTCTAAAATCTATATGATT +A00126:224:H7HMWDSX3:3:1105:14091:25864 Test_seq 170229 Test_seq 170533 + - UU A00126:224:H7HMWDSX3:3:1105:14091:2586497Test_seq17022960151M=170383305TAATATTGCTACTCCTCTTCTTTTTTGCTTATTATTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCGTGFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFF::FFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:77Yt:Z:UU A00126:224:H7HMWDSX3:3:1105:14091:25864145Test_seq17038360151M=170229-305CTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTTTTCATGTGAGTTGTTGGCCATTTCATTTTTCCACATACTTTTTTGTGCTGAGACGTTTTTCTTFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:2MD:Z:96T10T43MC:Z:151MAS:i:141XS:i:45Yt:Z:UU 60 60 TAATATTGCTACTCCTCTTCTTTTTTGCTTATTATTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCGTG CTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTTTTCATGTGAGTTGTTGGCCATTTCATTTTTCCACATACTTTTTTGTGCTGAGACGTTTTTCTT +A00126:224:H7HMWDSX3:3:1105:23719:28792 Test_seq 167149 Test_seq 167571 + - UU A00126:224:H7HMWDSX3:3:1105:23719:2879297Test_seq16714942151M=167439423ATTTGCATTTCTCTAATGGCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAAFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFF,FFFFFFFFFFFFFFF,F,:FFFFFFFFFFF:FFFF:FFNM:i:1MD:Z:94T56MC:Z:133M18SAS:i:146XS:i:126XA:Z:Test_seq,-213735,151M,5;Yt:Z:UU A00126:224:H7HMWDSX3:3:1105:23719:28792145Test_seq1674396133M18S=167149-423ATCGGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATGCCTTGTATTAGGGCTCCTAGGGTTGTCCCAATTTTTTCTTCCATGGTCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCGATCAGAACAAACCAGAGF:F:FFF::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:6MD:Z:3T47C22A0T2T15A38MC:Z:151MAS:i:104XS:i:99XA:Z:Test_seq,-400906,4S129M18S,6;Yt:Z:UU 42 6 ATTTGCATTTCTCTAATGGCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAA ATCGGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATGCCTTGTATTAGGGCTCCTAGGGTTGTCCCAATTTTTTCTTCCATGGTCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCGATCAGAACAAACCAGAG +A00126:224:H7HMWDSX3:3:1105:8024:29074 ! 0 Test_seq 167861 - + NR A00126:224:H7HMWDSX3:3:1105:8024:29074145Test_seq1681468125M26S=167861-410TTATTCCTAAGTATTTTATCTTCTTGGTGGCTACTGTGAATGGTATTGATTTGGTTATTTCCTCTTCGGTGTTCTTTTTGTTGATGTAGAGGAATCCAAGTGATTTTTGTATGTTTATTTTATAACCTGAAGGGGAAGTAAACATCATATTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FNM:i:2MD:Z:23C3G97MC:Z:151MAS:i:115XS:i:110XA:Z:Test_seq,+212763,21S130M,4;Yt:Z:NR A00126:224:H7HMWDSX3:3:1105:8024:2907497Test_seq16786160151M=168146410CTTTCTTCTTCTTTTTCAGTAATGCTTTGCTTATCCGGGGGTTCTTTCCCTTCCATATGAAATTGGTGATTTGTTTCTCTATCCCCTTAAAATATGACATTGGTATTTGGATTGGAAGTGCGTTATATGTATAGATGGCTTTTGGTAGAATFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFF:FFFFFFFFFFFFFFFFNM:i:1MD:Z:64A86MC:Z:125M26SAS:i:146XS:i:62Yt:Z:NR 0 60 CTTTCTTCTTCTTTTTCAGTAATGCTTTGCTTATCCGGGGGTTCTTTCCCTTCCATATGAAATTGGTGATTTGTTTCTCTATCCCCTTAAAATATGACATTGGTATTTGGATTGGAAGTGCGTTATATGTATAGATGGCTTTTGGTAGAAT +A00126:224:H7HMWDSX3:3:1105:24406:30389 Test_seq 33624 Test_seq 33928 + - UU A00126:224:H7HMWDSX3:3:1105:24406:30389161Test_seq336246019S132M=33778305ACTAAGACACATCACGATCGATCAGGAAACATGGGGAAAGTCTCCTTTCTTCAGTACAGGCAGCTCTCTCAGCTCCTTTTGGCTATGCAGGCCTCCTCTCAGCCCCCTCAGGACAGGCTTCCTCTTGACCCCCTCAGGACACACCTCCTGTFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F,FFFFF,FFNM:i:0MD:Z:132MC:Z:151MAS:i:132XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1105:24406:3038981Test_seq3377860151M=33624-305TCAGCCTGGCCTCTGCCCTGCTTGGGCAAGTGTTACAAAGCTGTTTAGCTCTGCCAGTAAGTGCCCGGAGGCACCCTACTGCACCAATAAGCCTTCTGTCTGAAGGTGTTCAGCTCTCCCACTCCATGGGCCAGCATACCCACTGTAACTGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFF::F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:19S132MAS:i:151XS:i:0Yt:Z:UU 60 60 ACTAAGACACATCACGATCGATCAGGAAACATGGGGAAAGTCTCCTTTCTTCAGTACAGGCAGCTCTCTCAGCTCCTTTTGGCTATGCAGGCCTCCTCTCAGCCCCCTCAGGACAGGCTTCCTCTTGACCCCCTCAGGACACACCTCCTGT TCAGCCTGGCCTCTGCCCTGCTTGGGCAAGTGTTACAAAGCTGTTTAGCTCTGCCAGTAAGTGCCCGGAGGCACCCTACTGCACCAATAAGCCTTCTGTCTGAAGGTGTTCAGCTCTCCCACTCCATGGGCCAGCATACCCACTGTAACTG +A00126:224:H7HMWDSX3:3:1105:18114:31422 ! 0 Test_seq 168033 - - MU A00126:224:H7HMWDSX3:3:1105:18114:31422177Test_seq2132360140M11S=167883-45343AGCCTGGTACTGGTACAACAACAGGCACATAGACCAATGGAACAGAATTGAGAACCCAGATATAAATCCATCCACGTATGAGCAGCTGATATTTGACAAAGGACCAGTGTCAGTCAATTGGGGAAATGATAGTCTTTTTATAAGTGCTCCCFFFFFFFFFFF:FFFFFFFFFFFFFF:F:FFFFFFFFFF:FFFF:FFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:2MD:Z:74T1A63MC:Z:151MAS:i:130XS:i:130XA:Z:Test_seq,+167663,11S140M,2;Yt:Z:MU A00126:224:H7HMWDSX3:3:1105:18114:31422113Test_seq16788360151M=21323645343TGCTTTGCTTATCCGGGGGTTCTTTCCCTTCCATATGAAATTAGTGATTTGTTTCTCTATCCCCTTAAAATATGACATTGGTATTTGGATTGGAAGTGCGTTATATGTATAGATGGCTTTTGGTAGAATAGACATTTTTACTATGTTAAGTFFF:F:FFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:140M11SAS:i:151XS:i:56Yt:Z:MU 0 60 AGCCTGGTACTGGTACAACAACAGGCACATAGACCAATGGAACAGAATTGAGAACCCAGATATAAATCCATCCACGTATGAGCAGCTGATATTTGACAAAGGACCAGTGTCAGTCAATTGGGGAAATGATAGTCTTTTTATAAGTGCTCCC TGCTTTGCTTATCCGGGGGTTCTTTCCCTTCCATATGAAATTAGTGATTTGTTTCTCTATCCCCTTAAAATATGACATTGGTATTTGGATTGGAAGTGCGTTATATGTATAGATGGCTTTTGGTAGAATAGACATTTTTACTATGTTAAGT +A00126:224:H7HMWDSX3:3:1105:14407:31955 Test_seq 175119 Test_seq 175607 + - UU A00126:224:H7HMWDSX3:3:1105:14407:3195597Test_seq17511960151M=175457489TTTCTCTCCTCAAGGAGAGGAACTAAAAAACATATGTAAACACACACACACACGAGTGTACACAACACAGAAGAGCAATGGCCCACAGCTCAGCTCTTTGATCCTTTCTGAAGCTGTGAGAGAATAGTATTCTTACCTCTTGGCTCATGAA:FF:FFFF,F:FFFFFF:FF:FFFFFFF::FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF::,F::FFFFFFFFFFFFFFFFFFFFFF,FFFFFFFF,FF,F:FFF:F:F:FFF,FFFF,:F,F:FFFFFFFF:FFFFFFF,FFFFNM:i:1MD:Z:124A26MC:Z:151MAS:i:146XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1105:14407:31955145Test_seq17545760151M=175119-489TTTCAGGTGACATCCTTTCAGATCTGGAAGCTGAGACTCAGTAAGGTGAGTGCCTGGCTCAGCATCCCTCAGCTAACAAGTCCCATAGGACTTGGAACTCCAGATTCTTCACTCTTTTTTCTAATATGCAAATGGTTTTCTGGGTTCCTAAFFFFF,::FF,FF:FFFFFFFFFFFFFF:FFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFF,:F,FFFFFF,:FFFF,:FFFF:F:FFFFFFFFFFFFFFFFFFF:FFFF:F:FF,FFF,FFFFFFF:FFFFFFF,FF,FFFFFFFFF:FFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU 60 60 TTTCTCTCCTCAAGGAGAGGAACTAAAAAACATATGTAAACACACACACACACGAGTGTACACAACACAGAAGAGCAATGGCCCACAGCTCAGCTCTTTGATCCTTTCTGAAGCTGTGAGAGAATAGTATTCTTACCTCTTGGCTCATGAA TTTCAGGTGACATCCTTTCAGATCTGGAAGCTGAGACTCAGTAAGGTGAGTGCCTGGCTCAGCATCCCTCAGCTAACAAGTCCCATAGGACTTGGAACTCCAGATTCTTCACTCTTTTTTCTAATATGCAAATGGTTTTCTGGGTTCCTAA +A00126:224:H7HMWDSX3:3:1105:8739:33317 Test_seq 167300 Test_seq 167662 + - UU A00126:224:H7HMWDSX3:3:1105:8739:33317161Test_seq16730019151M=167512363TCATGTAGATTTTAGAGATCAGGCACTGGTCTGAGATGTCATAGCTGAATATTCTTTCGCAGTCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCGGGTTTCTCFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:5MD:Z:23A0G33C9A73T8MC:Z:151MAS:i:126XS:i:116XA:Z:Test_seq,-213584,151M,7;Yt:Z:UU A00126:224:H7HMWDSX3:3:1105:8739:3331781Test_seq16751260151M=167300-363GATCCTATTTTTTCTTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGGTATGCCAGCACCATTTGT:FFFFFFFF:FFFF:FFFFFFFFF,FFFFFF:FFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:132T18MC:Z:151MAS:i:146XS:i:105Yt:Z:UU 19 60 TCATGTAGATTTTAGAGATCAGGCACTGGTCTGAGATGTCATAGCTGAATATTCTTTCGCAGTCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCGGGTTTCTC GATCCTATTTTTTCTTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGGTATGCCAGCACCATTTGT +A00126:224:H7HMWDSX3:3:1105:29604:34162 Test_seq 168625 Test_seq 212765 - - UU A00126:224:H7HMWDSX3:3:1105:29604:34162177Test_seq16847560151M=21261544141GGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:20T130MC:Z:151MAS:i:146XS:i:94Yt:Z:UU A00126:224:H7HMWDSX3:3:1105:29604:34162113Test_seq21261517151M=168475-44141CAGGCTAGACAAAGAAATAAAAGGTATCCGGATTGGCAAGGAAGAAGTAAAGTTATCACTATTTGCAGATGACATGATTATATACACAGAAAACCCTAAGGAATCCTCCAGAAAACTACTGAAACTAATAGAAGAGTTTGGCAGAGTCTCAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFNM:i:10MD:Z:0T20G2C3T13G8A5T20C0T17T53MC:Z:151MAS:i:105XS:i:95XA:Z:Test_seq,+168273,151M,12;Yt:Z:UU 60 17 GGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAA CAGGCTAGACAAAGAAATAAAAGGTATCCGGATTGGCAAGGAAGAAGTAAAGTTATCACTATTTGCAGATGACATGATTATATACACAGAAAACCCTAAGGAATCCTCCAGAAAACTACTGAAACTAATAGAAGAGTTTGGCAGAGTCTCA +A00126:224:H7HMWDSX3:3:1105:8296:34491 ! 0 Test_seq 168676 - - NR A00126:224:H7HMWDSX3:3:1105:8296:3449197Test_seq1683473426S125M=168526330GGTTTAATTTTTCAAAGCGTGATCGATCATGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCTTACCAATCTGGATACCCTTTATTTCTTTGTCTAGCCTAATTGCTCTGGCTAGGACTTCTAGCACAATGTTGAATAAGAGCGGTGAT:FFFFFFFF,FFF,FFFF:FFFFF:FFFF::FFFF:FFFFFFFF:FFF:FFFFFFFFFFFFFFFFFF::FFFFFFFFF::F:,FFFFFFFFFFFFFFFFFF::FFFF:FFFFF:::FFFFFFFF:F:FFFFFFFFFFFFFF,:FFFFFFFFNM:i:6MD:Z:18A21G41C14A2T2G21MC:Z:151MAS:i:95XS:i:77XA:Z:Test_seq,-212567,127M24S,10;Yt:Z:NR A00126:224:H7HMWDSX3:3:1105:8296:34491145Test_seq16852660151M=168347-330CCATTTCGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATCAFFFFFFFF:FFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF:FFFFF,FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:6A144MC:Z:26S125MAS:i:146XS:i:0Yt:Z:NR 0 60 CCATTTCGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATCA +A00126:224:H7HMWDSX3:3:1105:29441:34538 Test_seq 166096 Test_seq 166553 + - UU A00126:224:H7HMWDSX3:3:1105:29441:34538161Test_seq16609660151M=166403458TGTTGTGCTCCCTGACAGGGCAGACATCGATTGTGGCCGGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCTTAGTTGTCGTGTGACCTTGGTGTTCTTCCTTTGCCTFFF::FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFFFFFFF:FFFFFFF:FF:FFFFFF:FFFFFF:FFFFFF:FFFFFFFFF:F:F,FFFFFF:FFFF:FFF::FFFFFFFFFFFFFFFF:FF::F:FFFFFF,F,FF:FFNM:i:1MD:Z:128G22MC:Z:151MAS:i:146XS:i:83Yt:Z:UU A00126:224:H7HMWDSX3:3:1105:29441:3453881Test_seq16640360151M=166096-458CAGACCCCAGCCCCTGCTCCGCTGACCTTTGAAGCTTTCATTTTATCCCAGAAACCTCTTTGCTTTTAGTCCAGTCCAATTAGGCTGAACTTCCTTGTATTGAGTGTTGTCTTTCCCTTCACCCAAAGCAGTTCTTATCTACTAATTGATCFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFF:FFFFFFF,FFFFFFFFF:FFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:4MD:Z:49G38C13T40G7MC:Z:151MAS:i:131XS:i:37Yt:Z:UU 60 60 TGTTGTGCTCCCTGACAGGGCAGACATCGATTGTGGCCGGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCTTAGTTGTCGTGTGACCTTGGTGTTCTTCCTTTGCCT CAGACCCCAGCCCCTGCTCCGCTGACCTTTGAAGCTTTCATTTTATCCCAGAAACCTCTTTGCTTTTAGTCCAGTCCAATTAGGCTGAACTTCCTTGTATTGAGTGTTGTCTTTCCCTTCACCCAAAGCAGTTCTTATCTACTAATTGATC +A00126:224:H7HMWDSX3:3:1106:20039:2910 Test_seq 316262 Test_seq 316755 + - UU A00126:224:H7HMWDSX3:3:1106:20039:2910161Test_seq31626260149M2S=316605494GGTAAACATCCTTCTGGTATTCTCTGCTTTCAGCCAGGATCCATCTGACATCAGCAATGATATCCCTGGTTCCATGGCCTCTTCTGAAACCGGCCTGAATTTCTGGCAGTTCCCTGTCAATAAACTGCTGCAGCCGTTTTTGAATGATCAAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FF:FFFFFNM:i:0MD:Z:149MC:Z:151MAS:i:149XS:i:119Yt:Z:UU A00126:224:H7HMWDSX3:3:1106:20039:291081Test_seq31660560151M=316262-494TCTAGCCTCATGGCGAAGGAGGCAGTTGTTCACGGTGGCAATTAGCCACACTTTCCATATGGTCCTTCTGCTCCTGACGCTCCTTCTTCCTCTGTTGCTCCAGACAAATAGAGATCAATTGTTGTGCCTTGGGTGACCACTTGCAAGATTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:149M2SAS:i:151XS:i:0Yt:Z:UU 60 60 GGTAAACATCCTTCTGGTATTCTCTGCTTTCAGCCAGGATCCATCTGACATCAGCAATGATATCCCTGGTTCCATGGCCTCTTCTGAAACCGGCCTGAATTTCTGGCAGTTCCCTGTCAATAAACTGCTGCAGCCGTTTTTGAATGATCAA TCTAGCCTCATGGCGAAGGAGGCAGTTGTTCACGGTGGCAATTAGCCACACTTTCCATATGGTCCTTCTGCTCCTGACGCTCCTTCTTCCTCTGTTGCTCCAGACAAATAGAGATCAATTGTTGTGCCTTGGGTGACCACTTGCAAGATTT +A00126:224:H7HMWDSX3:3:1106:1651:2942 Test_seq 170100 Test_seq 170440 + - UU A00126:224:H7HMWDSX3:3:1106:1651:2942161Test_seq17010057151M=170290341CCTGTCATTGGGTGCATAAATATTTAATATGGTTATGTCTTCCTGATCAATTGTCCCTTTTATCATTATATAGTGTCCTTCTTTATCCTTTGTGGTGGATTTATGTCTAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCCTCTTCTTFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFF,FFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFF::FFFFFFFF,FFFFF:FFFFFF:FFFFFF,,FFF:FFFFFFF:,FFFFF,FFFF:FFNM:i:6MD:Z:7C7G20A58C0A6A47MC:Z:151MAS:i:121XS:i:90Yt:Z:UU A00126:224:H7HMWDSX3:3:1106:1651:294281Test_seq17029060151M=170100-341TTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCTGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAATFFFF,:FFFF,FFFF,FFFFFFFFFFFFFFFF:FFFFFFFFFF,FFFFFFFFF:FFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFF,FFFFFFFF,F:F:FFF,FFF,FFFFFFFFFFFFNM:i:1MD:Z:86C64MC:Z:151MAS:i:146XS:i:83Yt:Z:UU 57 60 CCTGTCATTGGGTGCATAAATATTTAATATGGTTATGTCTTCCTGATCAATTGTCCCTTTTATCATTATATAGTGTCCTTCTTTATCCTTTGTGGTGGATTTATGTCTAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCCTCTTCTT TTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCTGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAAT +A00126:224:H7HMWDSX3:3:1106:31051:3035 Test_seq 168737 Test_seq 168840 + - UU A00126:224:H7HMWDSX3:3:1106:31051:3035161Test_seq16873760104M=168737104TAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTCTATTGGCTAGAATTTTGTTGAGGATTTTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:104MC:Z:104MAS:i:104XS:i:49Yt:Z:UU A00126:224:H7HMWDSX3:3:1106:31051:303581Test_seq16873760104M=168737-104TAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTCTATTGGCTAGAATTTTGTTGAGGATTTTTFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:104MC:Z:104MAS:i:104XS:i:49Yt:Z:UU 60 60 TAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTCTATTGGCTAGAATTTTGTTGAGGATTTTT TAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTCTATTGGCTAGAATTTTGTTGAGGATTTTT +A00126:224:H7HMWDSX3:3:1106:29053:3709 Test_seq 166376 Test_seq 166553 + - UU A00126:224:H7HMWDSX3:3:1106:29053:370997Test_seq1663766015S136M=166403178GCCTCTGGTACTTTTTTAGAAGTCTCCTCAATCCATGTTCCCCAGACCCCAGCCCCTGCTCCGCTGACCTTTGAAACTTTCATTTTATCCCGGAAACCTCTTTGCTTTTAGTCCAGTCCAATTAGGCTGACCTTCCTTGTATTGTGTGTTGFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFF,F,FFFF:FFFFFFFFFFFF::FFF:FFFFFFFFFF:F:FFFFFFFFFFF:FFNM:i:2MD:Z:16A43G75MC:Z:151MAS:i:126XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1106:29053:3709145Test_seq16640360151M=166376-178CAGACCCCAGCCCCTGCTCCGCTGACCTTTGAAACTTTCATTTTATCCCGGAAACCTCTTTGCTTTTAGTCCAGTCCAATTAGGCTGACCTTCCTTGTATTGTGTGTTGTCTTTCCCTTCACCCAAAGCAGTTCTTATCTACTGATTGATCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:33G117MC:Z:15S136MAS:i:146XS:i:54Yt:Z:UU 60 60 GCCTCTGGTACTTTTTTAGAAGTCTCCTCAATCCATGTTCCCCAGACCCCAGCCCCTGCTCCGCTGACCTTTGAAACTTTCATTTTATCCCGGAAACCTCTTTGCTTTTAGTCCAGTCCAATTAGGCTGACCTTCCTTGTATTGTGTGTTG CAGACCCCAGCCCCTGCTCCGCTGACCTTTGAAACTTTCATTTTATCCCGGAAACCTCTTTGCTTTTAGTCCAGTCCAATTAGGCTGACCTTCCTTGTATTGTGTGTTGTCTTTCCCTTCACCCAAAGCAGTTCTTATCTACTGATTGATC +A00126:224:H7HMWDSX3:3:1106:11487:3787 Test_seq 168650 Test_seq 212761 - - UU A00126:224:H7HMWDSX3:3:1106:11487:3787113Test_seq16850060151M=21261144112TCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGGACTTTGTCAAATGCFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:136A14MC:Z:151MAS:i:146XS:i:78Yt:Z:UU A00126:224:H7HMWDSX3:3:1106:11487:3787177Test_seq21261116151M=168500-44112CAATCAGGCTAGACAAAGAAATAAAAGGTATCCGGATTGGCAAGGAAGAAGTAAAGTTATCACTATTTGCAGATGACATGATTATATACACAGAAAACCCTAAGGAATCCTCCAGAAAACTACTGAAACTAATAGAAGAGTTTGGCAGAGTFFFFF::F,FFF:FFFFFF,FFFFFFFFFFFFFFFFFFF:F:FFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:10MD:Z:4T20G2C3T13G8A5T20C0T17T49MC:Z:151MAS:i:101XS:i:91XA:Z:Test_seq,+168277,151M,12;Yt:Z:UU 60 16 TCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGGACTTTGTCAAATGC CAATCAGGCTAGACAAAGAAATAAAAGGTATCCGGATTGGCAAGGAAGAAGTAAAGTTATCACTATTTGCAGATGACATGATTATATACACAGAAAACCCTAAGGAATCCTCCAGAAAACTACTGAAACTAATAGAAGAGTTTGGCAGAGT +A00126:224:H7HMWDSX3:3:1106:7762:4163 Test_seq 170302 Test_seq 170653 + - UU A00126:224:H7HMWDSX3:3:1106:7762:4163161Test_seq17030260151M=170515352TTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGGGTAATTATAGATAAATAAGTTTTTAGTGFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:122C28MC:Z:83M12I56MAS:i:146XS:i:66Yt:Z:UU A00126:224:H7HMWDSX3:3:1106:7762:416381Test_seq1705156083M12I56M=170302-352GTGCTGAGGCGTTTTTCTTAGTAAATTGTGAGATCCTCATTTTCATAGTGTTTGACTTTATGTTAGTTGAGTCGTTACGTTTTTCTTGGCTTTTATCTTGAGTTATAGAGTTGTTATACCTTTTTGTGGTTACCTTATTATTTACCCCTATFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:21MD:Z:8A14G23A5A10T29G2A3A3T33MC:Z:151MAS:i:76XS:i:29Yt:Z:UU 60 60 TTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGGGTAATTATAGATAAATAAGTTTTTAGTG GTGCTGAGGCGTTTTTCTTAGTAAATTGTGAGATCCTCATTTTCATAGTGTTTGACTTTATGTTAGTTGAGTCGTTACGTTTTTCTTGGCTTTTATCTTGAGTTATAGAGTTGTTATACCTTTTTGTGGTTACCTTATTATTTACCCCTAT +A00126:224:H7HMWDSX3:3:1106:25735:4163 ! 0 Test_seq 423431 - + NR A00126:224:H7HMWDSX3:3:1106:25735:416381Test_seq42368060124M27S=423431-373CTAAACGAATACCATCCCAGACCCTCTGACATCTAACTACATTTAAGTGCACCATCTGATCTAAGAAAGCATACAAGTTCCCAATGTCTGGTGTCTTCTACCATGTTACTCATTATAAAGGACTAGTCTCTGTCCAAGTAGTCCAAAAAGT:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFNM:i:0MD:Z:124MC:Z:151MAS:i:124XS:i:0Yt:Z:NR A00126:224:H7HMWDSX3:3:1106:25735:4163161Test_seq42343160151M=423680373TCCTTCTTACTGTGGTTATGGCCATGATTTCAGCTTGAGAACCTAAGAAACTATTCACATAAAAATAATAACTTTCAAATTTATTCAAAACTCAAGTGAGGCCTTTAGTGTTGTAATATGGTTAGATAATAAAACAGATTCAAATATATTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:124M27SAS:i:151XS:i:0Yt:Z:NR 0 60 TCCTTCTTACTGTGGTTATGGCCATGATTTCAGCTTGAGAACCTAAGAAACTATTCACATAAAAATAATAACTTTCAAATTTATTCAAAACTCAAGTGAGGCCTTTAGTGTTGTAATATGGTTAGATAATAAAACAGATTCAAATATATTT +A00126:224:H7HMWDSX3:3:1106:13783:4977 Test_seq 167147 Test_seq 167535 + - UU A00126:224:H7HMWDSX3:3:1106:13783:497797Test_seq16714742151M=167403389TAATTTGCATTTCTCTAATGGCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:F:FFFFFFFFFFFF:FF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFF,FFFFFFFF:FFFFFFFF:FFF,FF,FFFF::FF,FFFF,F:FFFFFFFF:FFF:FFFFFFFNM:i:1MD:Z:96T54MC:Z:133M18SAS:i:146XS:i:126XA:Z:Test_seq,-213737,151M,5;Yt:Z:UU A00126:224:H7HMWDSX3:3:1106:13783:4977145Test_seq16740320133M18S=167147-389TGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCGGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATGCCTTGTATTAGGGCTCCTAGGGTTGTCCCTATTTTTTCTTCCATGATCACTCTACTTTGAGGAGGCFFFF,FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:4MD:Z:39T47C22A0T21MC:Z:151MAS:i:113XS:i:103XA:Z:Test_seq,+213499,18S133M,6;Yt:Z:UU 42 20 TAATTTGCATTTCTCTAATGGCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAG TGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCGGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATGCCTTGTATTAGGGCTCCTAGGGTTGTCCCTATTTTTTCTTCCATGATCACTCTACTTTGAGGAGGC +A00126:224:H7HMWDSX3:3:1106:28302:5948 Test_seq 168171 Test_seq 168613 + - UU A00126:224:H7HMWDSX3:3:1106:28302:594897Test_seq16817129151M=168463443GGGGGCTACTGTGAATGGCATTGATTTGGTTATTTCCTCTTCGGTGTTCTTTTTGTTGATGTAGAGGAATCCAAGTGATTTTTGTATTTTTATTTTATAACCTGAGACTCTTCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGACGATFF:FFFFF:F,F:FFFF:,F,FFFFFFF,FFFFFFFFFFFF:F,:FFFFFFFFF:FF,:F:FF,FFFF:F:FFFFFFF::FFF:FFF,FFFFFFFFFFF:FF,FF,FFFFFFFFFFFF::,FFF::FF,FFFF:FFF,FFFFFFF:F:FF:NM:i:4MD:Z:18T68G12T46G3MC:Z:151MAS:i:132XS:i:117XA:Z:Test_seq,-212717,151M,7;Yt:Z:UU A00126:224:H7HMWDSX3:3:1106:28302:5948145Test_seq16846360151M=168171-443AGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGFFFFFFFFFF,FFFFFF:FFF:FFFFFF:FFF,FFFFFFF,FFFFFFFFFF:FFFFFFFFFFFFFF:F:FFFFFFF:FFF:FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:FNM:i:1MD:Z:32T118MC:Z:151MAS:i:146XS:i:91Yt:Z:UU 29 60 GGGGGCTACTGTGAATGGCATTGATTTGGTTATTTCCTCTTCGGTGTTCTTTTTGTTGATGTAGAGGAATCCAAGTGATTTTTGTATTTTTATTTTATAACCTGAGACTCTTCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGACGAT AGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAG +A00126:224:H7HMWDSX3:3:1106:25427:6042 Test_seq 171093 Test_seq 171466 + - UU A00126:224:H7HMWDSX3:3:1106:25427:604297Test_seq17109360151M=171329374CCTTCAGTGCTCTGTATACGTCGTCCCATTCCCTTCTTGACTGCATGGTTTCTGCTGAGTAGTCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:,:,,FFFFFFFFFFF:FFFFF:FF,FFFFFFFFFFFFFFFFFFF:FFF:FFFFFNM:i:1MD:Z:39C111MC:Z:138M13SAS:i:146XS:i:58Yt:Z:UU A00126:224:H7HMWDSX3:3:1106:25427:6042145Test_seq17132960138M13S=171093-374CTTTCATCTTTCATGATGTCAGGGAAGTTTTCTGTCAGGAGTTCTTCAACTATTTTCTCTGTGTTTTCTGTCACCCCTCCCTGTTCTGGGACTCCAATCACTCGCAAGTTATCCTTCTCGATAGAGTTCCACATGATTAATCTAGAGCATGFFFFFFFFFFFFFFFFFFFFFF:F:FF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:7MD:Z:5G25G39G0C14T30T8C10MC:Z:151MAS:i:103XS:i:52Yt:Z:UU 60 60 CCTTCAGTGCTCTGTATACGTCGTCCCATTCCCTTCTTGACTGCATGGTTTCTGCTGAGTAGTCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTT CTTTCATCTTTCATGATGTCAGGGAAGTTTTCTGTCAGGAGTTCTTCAACTATTTTCTCTGTGTTTTCTGTCACCCCTCCCTGTTCTGGGACTCCAATCACTCGCAAGTTATCCTTCTCGATAGAGTTCCACATGATTAATCTAGAGCATG +A00126:224:H7HMWDSX3:3:1106:16152:6417 Test_seq 170871 Test_seq 171304 + - UU A00126:224:H7HMWDSX3:3:1106:16152:6417161Test_seq17087160151M=171154434GTTGCGTTGATATCAGGACGTTCTGTTTTGTGACCTTGCATTGTGCTGGTACCTGATATTATTGGTCATCAGGCCAAACAATCTCCTTTAGCATTTCTTGCAGTCTTGGTTTAGTTTTTGCAAATTCTCTAAACTTGTGTTTATCTGTAAAF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:F,FF,FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:FF:FFFNM:i:2MD:Z:38T49A62MC:Z:151MAS:i:141XS:i:23Yt:Z:UU A00126:224:H7HMWDSX3:3:1106:16152:641781Test_seq17115460151M=170871-434GTCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTGGGATCAATCTTAAATGGGGTTFFFFFFFFFFF:FFF:,FFFFFFF:FFF,FFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFF,FFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF::NM:i:1MD:Z:129T21MC:Z:151MAS:i:146XS:i:58Yt:Z:UU 60 60 GTTGCGTTGATATCAGGACGTTCTGTTTTGTGACCTTGCATTGTGCTGGTACCTGATATTATTGGTCATCAGGCCAAACAATCTCCTTTAGCATTTCTTGCAGTCTTGGTTTAGTTTTTGCAAATTCTCTAAACTTGTGTTTATCTGTAAA GTCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTGGGATCAATCTTAAATGGGGTT +A00126:224:H7HMWDSX3:3:1106:10221:9768 Test_seq 169867 Test_seq 170357 + - UU A00126:224:H7HMWDSX3:3:1106:10221:976897Test_seq16986760151M=170207491TGTGCGCTAGAAAAAAAAGTATACTTTGCAGCAGTTGGGTGGAGAGTTCTGTATAAGTCAATGAGGTCAAGTTGGTTGATTGTTGTAAGTAGGTCTTCAGTGTCTCTATTGAGCTTCTTACTGGATGTCCTGTCCTTCTCCGAAAGTGGTG:F,FFFFFFF:FF:FFFF:F,FF,FF:,FFF:FFFFFFFFFFF,FFFFFFFFFFFFFF:FFF:F:,,F,:FFF::FFFFFF:FF,FFF,:F::FFFF:,FF:F:F:FFF:F,FFFFFF,FFF,F::,FFFFFFFFFFFF:,FF,FFFFF,FNM:i:5MD:Z:5A17T35T38C8G43MC:Z:151MAS:i:126XS:i:38Yt:Z:UU A00126:224:H7HMWDSX3:3:1106:10221:9768145Test_seq17020760151M=169867-491TAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCCTCTTCTTTTTTGCTTATTGTTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATC:FFFFFF,:FFFF:,:F,FFFFFFF:FF:FFFFFFFFF:FFF::FF,,FFFFF:FFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFF:F::FFF:FFFFF:F:FFFF,:FFFFFFFFFFFFFFF:FFFFF:F:FF:FFFFFFF:FFFFFFFFNM:i:1MD:Z:55A95MC:Z:151MAS:i:146XS:i:88Yt:Z:UU 60 60 TGTGCGCTAGAAAAAAAAGTATACTTTGCAGCAGTTGGGTGGAGAGTTCTGTATAAGTCAATGAGGTCAAGTTGGTTGATTGTTGTAAGTAGGTCTTCAGTGTCTCTATTGAGCTTCTTACTGGATGTCCTGTCCTTCTCCGAAAGTGGTG TAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCCTCTTCTTTTTTGCTTATTGTTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATC +A00126:224:H7HMWDSX3:3:1106:12861:13933 Test_seq 171543 Test_seq 171937 + - UU A00126:224:H7HMWDSX3:3:1106:12861:1393397Test_seq17154360151M=171787395CCAGAAGTCCCAGTCTACATTCTAATTGCTCGAGTCTGCTCCTCTGACTTACTATTGCGTTGTCAAATTCTGTAATTTTATTGTTAATCTTTTGGATTTCTACATGCTGTCTCTCTATGGATTCTTGCAACTTGTTAATTTTTCCACTATGFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF,F,FFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFF:FFFFFFFF:FFFFF:FFFFFFNM:i:5MD:Z:40T9T13T68A14C2MC:Z:151MAS:i:128XS:i:60Yt:Z:UU A00126:224:H7HMWDSX3:3:1106:12861:13933145Test_seq17178760151M=171543-395AAGCATTCTGTAAATTAGTTTTTTATATTCTGTATCTGATAATTCCAGGATTGTATCTTCATTTGGGAAAGATTTTGATTCTTTTGTTTGGGGGGTTGGAGAAGCTGTCATGGTCTGTTTCTTTATGTGGTTTGATATGGGCTGCTGTCTCFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFFF:NM:i:1MD:Z:140A10MC:Z:151MAS:i:146XS:i:0Yt:Z:UU 60 60 CCAGAAGTCCCAGTCTACATTCTAATTGCTCGAGTCTGCTCCTCTGACTTACTATTGCGTTGTCAAATTCTGTAATTTTATTGTTAATCTTTTGGATTTCTACATGCTGTCTCTCTATGGATTCTTGCAACTTGTTAATTTTTCCACTATG AAGCATTCTGTAAATTAGTTTTTTATATTCTGTATCTGATAATTCCAGGATTGTATCTTCATTTGGGAAAGATTTTGATTCTTTTGTTTGGGGGGTTGGAGAAGCTGTCATGGTCTGTTTCTTTATGTGGTTTGATATGGGCTGCTGTCTC +A00126:224:H7HMWDSX3:3:1106:18954:14497 ! 0 Test_seq 167949 - + NR A00126:224:H7HMWDSX3:3:1106:18954:1449781Test_seq16822342123M28S=167949-397TTGTTGATGTAGAGGACTCCAAGTGATTTTTTTATGTTTATTTTATAACCTGAGACTCTTCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGGTTTTCCATGTATACGATCGATCTACGTCCAAAAAATCAGCCACFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF,FFFFFFFFFFFFF:FFFFFFF,:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFNM:i:3MD:Z:16A14G16T74MC:Z:151MAS:i:108XS:i:88XA:Z:Test_seq,+212703,38S113M,5;Yt:Z:NR A00126:224:H7HMWDSX3:3:1106:18954:14497161Test_seq16794960151M=168223397AAAATATGACATTGGTATTTGGATTGGAAGTGCGTTATATGTATAGATGGCTTTTGGTAGAATAGACATTTTTACTATGTTAAGTCTTCCTATCCATGAGCAGGGTATGTTTTTCCACTTAAGTATGTCCTTTTGAATTTCTTGTAGCAGAFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFNM:i:1MD:Z:111C39MC:Z:123M28SAS:i:146XS:i:80Yt:Z:NR 0 60 AAAATATGACATTGGTATTTGGATTGGAAGTGCGTTATATGTATAGATGGCTTTTGGTAGAATAGACATTTTTACTATGTTAAGTCTTCCTATCCATGAGCAGGGTATGTTTTTCCACTTAAGTATGTCCTTTTGAATTTCTTGTAGCAGA +A00126:224:H7HMWDSX3:3:1106:13404:16344 Test_seq 166073 Test_seq 166551 + - UU A00126:224:H7HMWDSX3:3:1106:13404:1634497Test_seq16607360151M=166401479CTCCTGCTCCCTCAGGAGTTGTCTGTTGTGCTCCCTGACAGGGCAGACATCGATTGTGGCCGGGCACCAACTAATTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCTTAGTTGTCGTGTGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF:FFFFNM:i:1MD:Z:73G77MC:Z:151MAS:i:146XS:i:74Yt:Z:UU A00126:224:H7HMWDSX3:3:1106:13404:16344145Test_seq16640160151M=166073-479CCCAGACCCCAGCCCCTGCTCCGCTGACCTTTGAAGCTTTCATTTTATCCCAGAAACCTCTTTGCTTTTAGTCCAGTCCAATTAGGCTGACATTCCTTGTATTGAGTGTTGTCTTTCCCTTCACCCAAAGCAGTTCTTATCTACAGATTGAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF,FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFNM:i:4MD:Z:51G39C12T39T6MC:Z:151MAS:i:131XS:i:37Yt:Z:UU 60 60 CTCCTGCTCCCTCAGGAGTTGTCTGTTGTGCTCCCTGACAGGGCAGACATCGATTGTGGCCGGGCACCAACTAATTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCTTAGTTGTCGTGTG CCCAGACCCCAGCCCCTGCTCCGCTGACCTTTGAAGCTTTCATTTTATCCCAGAAACCTCTTTGCTTTTAGTCCAGTCCAATTAGGCTGACATTCCTTGTATTGAGTGTTGTCTTTCCCTTCACCCAAAGCAGTTCTTATCTACAGATTGA +A00126:224:H7HMWDSX3:3:1106:18222:16736 Test_seq 168314 Test_seq 168630 + - UU A00126:224:H7HMWDSX3:3:1106:18222:1673697Test_seq16831411151M=168480317TGGAGGATTCCTTAGGGTTTTCTGTGTATATAATCATGTCATCTGCAAATAGTGATAACTTTACTTCTTCCTTGCCAATCCGGATACCTTTTATTTCTTTGTCTAGCCTGATTGCCCTGGCTAAGACTTCCAACACGATGTTGAATAAGAGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:F:F:FFFFFNM:i:15MD:Z:22C0A6C0G0G18A5G9C12T7C20A13G6A1G0T17MC:Z:151MAS:i:76XS:i:68XA:Z:Test_seq,-212574,151M,17;Yt:Z:UU A00126:224:H7HMWDSX3:3:1106:18222:16736145Test_seq16848060151M=168314-317TCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGG:FFFFFFFFFFFFFFFFF,F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:15T135MC:Z:151MAS:i:146XS:i:91Yt:Z:UU 11 60 TGGAGGATTCCTTAGGGTTTTCTGTGTATATAATCATGTCATCTGCAAATAGTGATAACTTTACTTCTTCCTTGCCAATCCGGATACCTTTTATTTCTTTGTCTAGCCTGATTGCCCTGGCTAAGACTTCCAACACGATGTTGAATAAGAG TCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGG +A00126:224:H7HMWDSX3:3:1106:18747:17018 Test_seq 170380 Test_seq 170837 + - UU A00126:224:H7HMWDSX3:3:1106:18747:17018161Test_seq17038060151M=170691458ACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGGGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTTTTCATGTGTGTTGTTGGCCTTTTCATTTTTCCACATACTTTTTTGTGCTGAGACGTTTTTFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF,FFFNM:i:1MD:Z:44C106MC:Z:73M4I74MAS:i:146XS:i:53Yt:Z:UU A00126:224:H7HMWDSX3:3:1106:18747:1701881Test_seq1706916073M4I74M=170380-458CACCTTGTATCACTCTCCATCTGGCAGTTCAATGCCTCCTATATTTAGTCCCTCTTTTTGATTATTGTGATCGATCATTTATCTATTGATTTCCATGATTTCCTGTTATGTGTATTATTTTGTTTATTTATTTATTTTTTAGAATTAATCTFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF:F,FFFF:FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:5MD:Z:40G106MC:Z:151MAS:i:132XS:i:0Yt:Z:UU 60 60 ACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGGGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTTTTCATGTGTGTTGTTGGCCTTTTCATTTTTCCACATACTTTTTTGTGCTGAGACGTTTTT CACCTTGTATCACTCTCCATCTGGCAGTTCAATGCCTCCTATATTTAGTCCCTCTTTTTGATTATTGTGATCGATCATTTATCTATTGATTTCCATGATTTCCTGTTATGTGTATTATTTTGTTTATTTATTTATTTTTTAGAATTAATCT +A00126:224:H7HMWDSX3:3:1106:16251:18740 Test_seq 225248 Test_seq 308334 + + UR A00126:224:H7HMWDSX3:3:1106:16251:18740129Test_seq2252486029M122S=30833483087GTTCTGATGTTGTTGTCATTTACAGATTATAAGTCAATCTCTTTTGAGATATAAAAGAGAGAAGTGAGGAGAAAGACATGGAGAACTCATGCTACCAAGAAACAAGATCCAGGAGAATAGCATGTCCTTTGGACCCAGGGTCCCCGTGGCTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF,FF,FFFFFFFFFFFFF:FFNM:i:0MD:Z:29MC:Z:151MAS:i:29XS:i:0SA:Z:Test_seq,308589,-,122M29S,60,0;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1106:16251:187402193Test_seq30858960122M29H=308334-377AGCCACGGGGACCCTGGGTCCAAAGGACATGCTATTCTCCTGGATCTTGTTTCTTGGTAGCATGAGTTCTCCATGTCTTTCTCCTCACTTCTCTCTTTTATATCTCAAAAGAGATTGACTTAFF:FFFFFFFFFFFFF,FF,FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFNM:i:0MD:Z:122MC:Z:151MAS:i:122XS:i:24SA:Z:Test_seq,225248,+,29M122S,60,0;Yt:Z:UR A00126:224:H7HMWDSX3:3:1106:16251:1874065Test_seq30833460151M=225248-83087TCCATAACATACCACTGTAGTGCCCCACATAGAATCCATTTTTATAATATGCTTAGCTTAGTTGTCTTAGTTATCCAGTGCTGCTATAACAGAAATAACACAAGTGGATGGCTTTAACAAACAGAAAGTTATTCTCTACAGTCTAGGAGGCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:29M122SAS:i:151XS:i:35Yt:Z:UR 60 60 GTTCTGATGTTGTTGTCATTTACAGATTATAAGTCAATCTCTTTTGAGATATAAAAGAGAGAAGTGAGGAGAAAGACATGGAGAACTCATGCTACCAAGAAACAAGATCCAGGAGAATAGCATGTCCTTTGGACCCAGGGTCCCCGTGGCT TCCATAACATACCACTGTAGTGCCCCACATAGAATCCATTTTTATAATATGCTTAGCTTAGTTGTCTTAGTTATCCAGTGCTGCTATAACAGAAATAACACAAGTGGATGGCTTTAACAAACAGAAAGTTATTCTCTACAGTCTAGGAGGC +A00126:224:H7HMWDSX3:3:1106:31901:18756 Test_seq 56593 Test_seq 115194 + + RU A00126:224:H7HMWDSX3:3:1106:31901:18756129Test_seq5659360151M=11519458602CTTCCGTATGGATATTATCCTATCACTGACAGCATTTTACTTCAGGATAGATCTTGAAATGTTCTTTTTGATGATGAATGCAATGCCATTCCTCTTCAAGTTGTCATTCCCAGCATAGTAGACCACATGATTGTCTGATCCAAAATGGCCAFFFFFF,,FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFF:F:FFFFFF:FFFFFFFFFFF:,FFFFFFFFFFFFF:,FFFFFFF:NM:i:0MD:Z:151MC:Z:5S23M123SAS:i:151XS:i:103Yt:Z:RU A00126:224:H7HMWDSX3:3:1106:31901:187562129Test_seq5681660123M28H=56593-346TCCAACTTTCCTAGATTTATATTTTGTACATTCCATATTCTGATTATTACTGAATGTTTGCAGCTATTTCTTCTCATTTTGAGTTATGCCATATCAGTAAATGAAGGTCCCAAAAGCTTGACTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFF,F:FFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:123MC:Z:151MAS:i:123XS:i:0SA:Z:Test_seq,115194,+,5S23M123S,24,0;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1106:31901:1875665Test_seq115194245S23M123S=56593-58602TGCTCTGTCACATGGGGTTGCTATGAGTAGTCAAGCTTTTGGGACCTTCATTTACTGATATGGCATAACTCAAAATGAGAAGAAATAGCTGCAAACATTCAGTAATAATCAGAATATGGAATGTACAAAATATAAATCTAGGAAAGTTGGAFFFFFFFFF:FF,FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFF:F,FFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:23MC:Z:151MAS:i:23XS:i:0SA:Z:Test_seq,56816,-,123M28S,60,0;Yt:Z:RU 60 24 CTTCCGTATGGATATTATCCTATCACTGACAGCATTTTACTTCAGGATAGATCTTGAAATGTTCTTTTTGATGATGAATGCAATGCCATTCCTCTTCAAGTTGTCATTCCCAGCATAGTAGACCACATGATTGTCTGATCCAAAATGGCCA TGCTCTGTCACATGGGGTTGCTATGAGTAGTCAAGCTTTTGGGACCTTCATTTACTGATATGGCATAACTCAAAATGAGAAGAAATAGCTGCAAACATTCAGTAATAATCAGAATATGGAATGTACAAAATATAAATCTAGGAAAGTTGGA +A00126:224:H7HMWDSX3:3:1106:24795:22357 Test_seq 169327 Test_seq 169680 + - UU A00126:224:H7HMWDSX3:3:1106:24795:22357161Test_seq16932760151M=169530354GCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGATTCTTTTGTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF,FFFF,FFFFF:FFFF:FF:FFFF:FFFFFFFFFFFFFFNM:i:1MD:Z:140G10MC:Z:151MAS:i:146XS:i:111Yt:Z:UU A00126:224:H7HMWDSX3:3:1106:24795:2235781Test_seq16953057151M=169327-354TTCTTCTTTTTGTATGTGTGCATTTATTGATATAAATTGACCTCTGAGCACTGCTTTTGCTGTGTCCCAGAGGTTTTGATAGGAAGTATTTTCATTCTCGTTGCAGCCTATGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:8MD:Z:27C11G58T5T0T0T3A22G17MC:Z:151MAS:i:111XS:i:78Yt:Z:UU 60 57 GCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGATTCTTTTGTT TTCTTCTTTTTGTATGTGTGCATTTATTGATATAAATTGACCTCTGAGCACTGCTTTTGCTGTGTCCCAGAGGTTTTGATAGGAAGTATTTTCATTCTCGTTGCAGCCTATGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAG +A00126:224:H7HMWDSX3:3:1106:9127:23187 Test_seq 168739 Test_seq 169233 + - UU A00126:224:H7HMWDSX3:3:1106:9127:23187161Test_seq16873960151M=169083495AACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTCTATTGGCTAGAATTTTGTTGAGGATTTTTGCATCTATGTTCATGAGGGATATAGGTCTAAAATTTTCTTTTTTTGTAAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FF:NM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:81Yt:Z:UU A00126:224:H7HMWDSX3:3:1106:9127:2318781Test_seq16908360151M=168739-495TTCAATCTGTTTTTTTGTTATGGGTCTATTTAGTTGTTCTACTTCTGAATGTGTTAGTTTAGGTAGGTAGTATTGTTCCAAGAATTTATCCATTTCTTCTAGGTTTTCAAATTTGTTAGAGTACAATTTTACGTAGTAATCTGAAGTGATTFFF,F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:7MD:Z:8C62G2T3G7C31C26A5MC:Z:151MAS:i:116XS:i:65Yt:Z:UU 60 60 AACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTCTATTGGCTAGAATTTTGTTGAGGATTTTTGCATCTATGTTCATGAGGGATATAGGTCTAAAATTTTCTTTTTTTGTAA TTCAATCTGTTTTTTTGTTATGGGTCTATTTAGTTGTTCTACTTCTGAATGTGTTAGTTTAGGTAGGTAGTATTGTTCCAAGAATTTATCCATTTCTTCTAGGTTTTCAAATTTGTTAGAGTACAATTTTACGTAGTAATCTGAAGTGATT +A00126:224:H7HMWDSX3:3:1106:17309:23328 Test_seq 168424 Test_seq 168810 + - UU A00126:224:H7HMWDSX3:3:1106:17309:23328161Test_seq16842460151M=168660387ATTGCCCTGGCTACGACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCCGTTGGCTTTGCATAGATGCCCTTTATTAF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:3MD:Z:13G57T50T28MC:Z:151MAS:i:136XS:i:67Yt:Z:UU A00126:224:H7HMWDSX3:3:1106:17309:2332881Test_seq16866060151M=168424-387ATCTATTGATAAGATCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:56T94MC:Z:151MAS:i:146XS:i:55Yt:Z:UU 60 60 ATTGCCCTGGCTACGACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCCGTTGGCTTTGCATAGATGCCCTTTATTA ATCTATTGATAAGATCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATT +A00126:224:H7HMWDSX3:3:1106:17707:24392 Test_seq 168738 Test_seq 169130 + - UU A00126:224:H7HMWDSX3:3:1106:17707:2439297Test_seq16873860151M=168980393AAACCAGCCTTGCAGACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGCATTCTATTGGCTAGAATTTTGTTGAGGATTTTTGCATCTATGTTCATGAGGGATATAGGTCTAAAATTTTCTTTTTTTGTAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF,FFFFFF:FFFFFF:FFFFFFFFF,FFFFFFFFFFFFFFFFFNM:i:2MD:Z:14T54G81MC:Z:151MAS:i:141XS:i:81Yt:Z:UU A00126:224:H7HMWDSX3:3:1106:17707:24392145Test_seq16898060151M=168738-393TCAGTAGTAGTGGTGTTAAGTCTTCTCTGAAGGTTTGGTAGAACTCTGCAGTGAAGCCGTCTGGGCCAGGACTTTTTTTTGTTGGAAGTTTTTTGATTACCGTTCCAATCTCTTTTTTTGTTATGGGTCTATTTAGTTGTTCTACTTCTGAFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFNM:i:1MD:Z:104T46MC:Z:151MAS:i:146XS:i:63Yt:Z:UU 60 60 AAACCAGCCTTGCAGACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGCATTCTATTGGCTAGAATTTTGTTGAGGATTTTTGCATCTATGTTCATGAGGGATATAGGTCTAAAATTTTCTTTTTTTGTA TCAGTAGTAGTGGTGTTAAGTCTTCTCTGAAGGTTTGGTAGAACTCTGCAGTGAAGCCGTCTGGGCCAGGACTTTTTTTTGTTGGAAGTTTTTTGATTACCGTTCCAATCTCTTTTTTTGTTATGGGTCTATTTAGTTGTTCTACTTCTGA +A00126:224:H7HMWDSX3:3:1106:27308:24862 Test_seq 167312 Test_seq 214100 - - UU A00126:224:H7HMWDSX3:3:1106:27308:24862177Test_seq16716252151M=21395046789TAATGGCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAATCATGTAGATTTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:81T69MC:Z:151MAS:i:146XS:i:121XA:Z:Test_seq,+213722,151M,6;Yt:Z:UU A00126:224:H7HMWDSX3:3:1106:27308:24862113Test_seq21395037151M=167162-46789AATAAATGTTGGAGAGGCTGCGGAGAGATTGGAACTCTTATACGCTGCTGGTGGGAATGTAAAATGGTACAACCACTTTGGAAATCTATCTGGCGTTTTCTTAAACAGTTAGAAATAGAACTACCATACAACCCAGAAATCCCACTCCTCGF:F:::FFFFFFFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:4MD:Z:28C14A49T11A45MC:Z:151MAS:i:131XS:i:112XA:Z:Test_seq,+166938,4S147M,7;Yt:Z:UU 52 37 TAATGGCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAATCATGTAGATTTT AATAAATGTTGGAGAGGCTGCGGAGAGATTGGAACTCTTATACGCTGCTGGTGGGAATGTAAAATGGTACAACCACTTTGGAAATCTATCTGGCGTTTTCTTAAACAGTTAGAAATAGAACTACCATACAACCCAGAAATCCCACTCCTCG +A00126:224:H7HMWDSX3:3:1106:2284:29434 ! 0 Test_seq 167543 - - NR A00126:224:H7HMWDSX3:3:1106:2284:2943497Test_seq1671734428S123M=167393371GTGAAGATGCATCTACATGCCAAGGATCGATCGGGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGAC:FFFFF:::FF:F:FFFF::F:,F:,F:FFFFFF:F,:FFFFF:FFFFFFFF:FFFFFFFFF,:FF,FFFF:FF,FFFF,FF,F:F:F:,FFF:FFFF,:FFF:F::F,F,:F,F,FFF:FFFFFFFFF,FFFF:,F::FFF,FFFFFFF,NM:i:2MD:Z:5A64T52MC:Z:151MAS:i:113XS:i:93XA:Z:Test_seq,-213739,123M28S,6;Yt:Z:NR A00126:224:H7HMWDSX3:3:1106:2284:29434145Test_seq16739360151M=167173-371AGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATGCCCTGTATTAGGGCTCCTAGGGTAGATCCTATTTTTTCTTCCATGATCTTTATCGTFF:FFFFFFFFF:,,FFFF:,FFFFFFFFFFFFF:FFFFFF,F,FFFF,FFFF:FF:FFF:FF:FF:F:FF,FFFFFFFFF:FFFFFF,:FFFFF,,FFFFFFFF,:FFFFFFFF,F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FNM:i:1MD:Z:118T32MC:Z:28S123MAS:i:146XS:i:117XA:Z:Test_seq,+213491,29M4D4M4I114M,10;Yt:Z:NR 0 60 AGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATGCCCTGTATTAGGGCTCCTAGGGTAGATCCTATTTTTTCTTCCATGATCTTTATCGT +A00126:224:H7HMWDSX3:3:1106:3125:29763 Test_seq 169989 Test_seq 170460 + - UU A00126:224:H7HMWDSX3:3:1106:3125:29763161Test_seq16998948151M=170310472GGATGTCCTGTCCTTCTCCGAAAGTGGTGTGTTAAAGTCTCCTACTATAATTGTGGAGGTATCTATATCACTTTTCAATTCTGTTAAAATTTGATTTATGTATCTTGCAGCCCTGTCATTGGGTGCATAAATATTTAATATGGTTATTTCT,FFFF:FFF,FF,:FF:F,,FFFFF::F,FF,:F:FF:FFF,FFFFFFF,FFFF,FFFF:FFFFFFFFFFF:FFF:FFFFFF,F:F:F:FFFFFFFFFF,FFF,FF,F,F,,:F,F:FFFF,,FFFFFFFFFFFFFFFFFFF,FFFF,::FNM:i:10MD:Z:33G5C10A7A1G5C2G48C7G20A3MC:Z:151MAS:i:102XS:i:73Yt:Z:UU A00126:224:H7HMWDSX3:3:1106:3125:2976381Test_seq17031060151M=169989-472GTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCTGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATTFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:66C84MC:Z:151MAS:i:146XS:i:63Yt:Z:UU 48 60 GGATGTCCTGTCCTTCTCCGAAAGTGGTGTGTTAAAGTCTCCTACTATAATTGTGGAGGTATCTATATCACTTTTCAATTCTGTTAAAATTTGATTTATGTATCTTGCAGCCCTGTCATTGGGTGCATAAATATTTAATATGGTTATTTCT GTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCTGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATT +A00126:224:H7HMWDSX3:3:1106:29026:29841 ! 0 Test_seq 166159 - + MR A00126:224:H7HMWDSX3:3:1106:29026:298412129Test_seq16642960128M23H=166159-398CTTTGAAGCTTTCATTTTATCCCGGAAACCTCTTTGCTTTTAGTCCAGTCCAATTAGGCTGACCTTCCTTGTATTGTGTGTTGTCTTTCCCTTCATCCAAAGCAGTTCTTATCTCCTGATTGATCAATFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:2MD:Z:95C18A13MC:Z:151MAS:i:118XS:i:28SA:Z:Test_seq,383472,+,5S21M125S,0,0;Yt:Z:MRNEXT_SAMA00126:224:H7HMWDSX3:3:1106:29026:2984165Test_seq38347205S21M125S=166159-217314GCCTTGAGTTGCAATAGTGAAGGATTGATCAATCAGGAGATAAGAACTGCTTTGGATGAAGGGAAAGACAACACACAATACAAGGAAGGTCAGCCTAATTGGACTGGACTAAAAGCAAAGAGGTTTCCGGGATAAAATGAAAGCTTCAAAGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:21MC:Z:151MAS:i:21XS:i:21SA:Z:Test_seq,166429,-,128M23S,60,2;XA:Z:Test_seq,-334091,125S26M,1;Yt:Z:MR A00126:224:H7HMWDSX3:3:1106:29026:29841129Test_seq16615960151M=383472217314CAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCTTAGTTGTCGTGTGGCCTTGGTGTTCTTCCTTTGCCTTTGCTCCAGGTGGGGTGAGACCAATTGATGTATCTTAGATGGCCGCTTGTTGGCATTTAGGACFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFFF:FFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF,FFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:::FFFFFFNM:i:1MD:Z:102T48MC:Z:5S21M125SAS:i:146XS:i:77Yt:Z:MR 0 60 GCCTTGAGTTGCAATAGTGAAGGATTGATCAATCAGGAGATAAGAACTGCTTTGGATGAAGGGAAAGACAACACACAATACAAGGAAGGTCAGCCTAATTGGACTGGACTAAAAGCAAAGAGGTTTCCGGGATAAAATGAAAGCTTCAAAG CAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCTTAGTTGTCGTGTGGCCTTGGTGTTCTTCCTTTGCCTTTGCTCCAGGTGGGGTGAGACCAATTGATGTATCTTAGATGGCCGCTTGTTGGCATTTAGGAC +A00126:224:H7HMWDSX3:3:1106:15591:30123 Test_seq 171134 Test_seq 171455 + - UU A00126:224:H7HMWDSX3:3:1106:15591:3012397Test_seq17113460151M=171323322TGCATGGTTTCTGCTGAGTAGTCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTCGATGATAATATGTCTTGGTGTTTTTCTTTTTG:FF:FFFFFF:,FF:F:F,,FF:F:F,F::,FFFFF,FFFF,:::::F:FFFFFFF,,FF::F:F:FFFFFFF:FFFFFF,,F,FFF,:FFFF:F,,:F,F,,,FF:,::FFF,,FF,FF,,FF:F,,,FF,FF,FFFF,F,FFFF,F,:FNM:i:1MD:Z:118T32MC:Z:133M18SAS:i:146XS:i:63Yt:Z:UU A00126:224:H7HMWDSX3:3:1106:15591:30123145Test_seq17132360133M18S=171134-322GATATCCTTTCGTCTTTCATGATGTCAGGGAAGTTTTGTGTCAGGATTTCTTCAACTATTTTCTCTGTGTTTTCTGTCCCCCCTCCCTGTTCTGGGACTCCAATCACTCGCAAGTTATCCTTCTTGATAGAGTAATCATGGCCCAGCCAAG::::,FFF,:F:FFF,FFF:FF,:FF:FF:FF:FF,F:,F:F:::FF::F,FFFFF,,,,,FF:F::::F,,FF,F,FFFFFF:FFF,F:FF:F::FF,FF,FFF,F,FFFFFF,F,:FF,FFFF:F,FFFFFF:FF::FFFFFF:FF:,FNM:i:3MD:Z:46G30G15T39MC:Z:151MAS:i:118XS:i:38Yt:Z:UU 60 60 TGCATGGTTTCTGCTGAGTAGTCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTCGATGATAATATGTCTTGGTGTTTTTCTTTTTG GATATCCTTTCGTCTTTCATGATGTCAGGGAAGTTTTGTGTCAGGATTTCTTCAACTATTTTCTCTGTGTTTTCTGTCCCCCCTCCCTGTTCTGGGACTCCAATCACTCGCAAGTTATCCTTCTTGATAGAGTAATCATGGCCCAGCCAAG +A00126:224:H7HMWDSX3:3:1106:30138:30483 Test_seq 183328 Test_seq 183714 + - UU A00126:224:H7HMWDSX3:3:1106:30138:30483161Test_seq1833286026M4I121M=183564387TGTTCTGCATCTATTAAAAATGATGAGATCGATCCATGTAGCTGTAGCAGTGGTGGGTTAGAGTTTTAGGTCTGTAGCCAGTAGATGTATGTTTGTATTTTCTCTCTGCCACTTACTAGTTGTGTTAATTTGAGCAAGTCATTTGACCTTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF,FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFF::FFFFFFNM:i:4MD:Z:147MC:Z:151MAS:i:137XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1106:30138:3048381Test_seq18356460151M=183328-387TGGTTCCGACTCATAGCATCCCTATGTACAACAGAATGAAACGCTGCCCTGTCCTGTGCCAGCCTTGCAATCATTGTTATGCTTGATCCCATTGTTGTAGCCACTGTATCAATCCATCTCATTGAGGGTCTTGCTCTTTTTCACTGACCCTFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFF:,FFFFFFFFF,FF:FFFFFFFFFFFFFFFFFFFFFFFFF:FF,FFFFFF:FFFFF:FFFFFFF:FFF:FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:26M4I121MAS:i:151XS:i:52Yt:Z:UU 60 60 TGTTCTGCATCTATTAAAAATGATGAGATCGATCCATGTAGCTGTAGCAGTGGTGGGTTAGAGTTTTAGGTCTGTAGCCAGTAGATGTATGTTTGTATTTTCTCTCTGCCACTTACTAGTTGTGTTAATTTGAGCAAGTCATTTGACCTTT TGGTTCCGACTCATAGCATCCCTATGTACAACAGAATGAAACGCTGCCCTGTCCTGTGCCAGCCTTGCAATCATTGTTATGCTTGATCCCATTGTTGTAGCCACTGTATCAATCCATCTCATTGAGGGTCTTGCTCTTTTTCACTGACCCT +A00126:224:H7HMWDSX3:3:1106:15890:30639 Test_seq 171134 Test_seq 171455 + - UU A00126:224:H7HMWDSX3:3:1106:15890:3063997Test_seq17113460151M=171323322TGCATGGTTTCTGCTGAGTAGTCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTCGATGATAATATGTCTTGGTGTTTTTCTTTTTGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FF,::FFFFFFFFFFFF,:FF:F:FFFF,FFFFFF:FF:FFFF:FFFF:FFFFFFFFFFFFFFFFFFFFF:FFNM:i:1MD:Z:118T32MC:Z:133M18SAS:i:146XS:i:63Yt:Z:UU A00126:224:H7HMWDSX3:3:1106:15890:30639145Test_seq17132360133M18S=171134-322GATATCCTTTCGTCTTTCATGATGTCAGGGAAGTTTTGTGTCAGGATTTCTTCAACTATTTTCTCTGTGTTTTCTGTCCCCCCTCCCTGTTCTGGGACTCCAATCACTCGCAAGTTATCCTTCTTGATAGAGTAATCATGGCCCAGCCAAGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFF:FF,:FFFFFFFFFFFFFFFFFFFFFFNM:i:3MD:Z:46G30G15T39MC:Z:151MAS:i:118XS:i:38Yt:Z:UU 60 60 TGCATGGTTTCTGCTGAGTAGTCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTCGATGATAATATGTCTTGGTGTTTTTCTTTTTG GATATCCTTTCGTCTTTCATGATGTCAGGGAAGTTTTGTGTCAGGATTTCTTCAACTATTTTCTCTGTGTTTTCTGTCCCCCCTCCCTGTTCTGGGACTCCAATCACTCGCAAGTTATCCTTCTTGATAGAGTAATCATGGCCCAGCCAAG +A00126:224:H7HMWDSX3:3:1106:15881:31344 ! 0 Test_seq 168469 - + NR A00126:224:H7HMWDSX3:3:1106:15881:31344145Test_seq16855160125M26S=168469-207GCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCAATTGATAAGATCGATCTCTCCCTCCTTTTATCCTTCCT:::FF:FF,FFFF,FFFF:F,::FFFFFFFFFFFFF:FFFFFFFF:FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFNM:i:1MD:Z:112T12MC:Z:151MAS:i:120XS:i:70Yt:Z:NR A00126:224:H7HMWDSX3:3:1106:15881:3134497Test_seq16846960151M=168551207GATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTAFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFF,FF:FFFFFFFFFFFFFFFFFFFFFFF,FFF:FFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:26T124MC:Z:125M26SAS:i:146XS:i:94Yt:Z:NR 0 60 GATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTA +A00126:224:H7HMWDSX3:3:1106:9118:32095 Test_seq 171491 Test_seq 171935 + - UU A00126:224:H7HMWDSX3:3:1106:9118:3209597Test_seq17149160151M=171785445ATTCTTTTATCTGATTTTTTTTCAGCTATGTTGGTGTTGTTTCCCTGGTCCTCCAGAAGTCCCAGTCTACATTCTAATTGCTCGAGTCTGCTCCTCTGACTTTCTATTGCGTTGTCAATTTCTGTAATTTTATTGTTAATCTTTTGGATTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:F:FFF:FFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFNM:i:5MD:Z:34A4A52T23T1A32MC:Z:151MAS:i:126XS:i:42Yt:Z:UU A00126:224:H7HMWDSX3:3:1106:9118:32095145Test_seq17178560151M=171491-445TTAAGCATTCTGTAAATTAGTTTTTTATATTCTGTATCTGATAATTCCAGGATTGTATCTTCATTTGGGAAAGATTTTGATTCTTTTGTTGGGGGGGTTGGAGAAGCTGTCATGGTCTGTTTCTTTATGTGGTTTGATATGGACTGCTGTCFFFFFFFFFFFFF:FFFF,FFFFFFFFFFFFFFFF,FFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:90T60MC:Z:151MAS:i:146XS:i:25Yt:Z:UU 60 60 ATTCTTTTATCTGATTTTTTTTCAGCTATGTTGGTGTTGTTTCCCTGGTCCTCCAGAAGTCCCAGTCTACATTCTAATTGCTCGAGTCTGCTCCTCTGACTTTCTATTGCGTTGTCAATTTCTGTAATTTTATTGTTAATCTTTTGGATTT TTAAGCATTCTGTAAATTAGTTTTTTATATTCTGTATCTGATAATTCCAGGATTGTATCTTCATTTGGGAAAGATTTTGATTCTTTTGTTGGGGGGGTTGGAGAAGCTGTCATGGTCTGTTTCTTTATGTGGTTTGATATGGACTGCTGTC +A00126:224:H7HMWDSX3:3:1106:14362:33599 Test_seq 167148 Test_seq 167571 + - UU A00126:224:H7HMWDSX3:3:1106:14362:3359997Test_seq16714841151M=167421424AATTTGCATTTCTCTAATGGCTAATGATCGGGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF::FF,FFFF:FFF:FFFFFF,F,FFFFFFF,F:FFFF,:FFFNM:i:2MD:Z:30A64T55MC:Z:151MAS:i:141XS:i:121XA:Z:Test_seq,-213736,151M,6;Yt:Z:UU A00126:224:H7HMWDSX3:3:1106:14362:33599145Test_seq16742160151M=167148-424TTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATGCCCTGTATTAGGGCTCCTGGGGTTGATCCTATTTTTTCTTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,F:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F,FFFFFFFFFFFFFFFFFFF:FF:,FFFFFFFFFFFF:FFFFF:F,FFFFFFNM:i:1MD:Z:85A65MC:Z:151MAS:i:146XS:i:106Yt:Z:UU 41 60 AATTTGCATTTCTCTAATGGCTAATGATCGGGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGA TTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATGCCCTGTATTAGGGCTCCTGGGGTTGATCCTATTTTTTCTTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATC +A00126:224:H7HMWDSX3:3:1106:27236:35697 Test_seq 406947 Test_seq 407376 + - UU A00126:224:H7HMWDSX3:3:1106:27236:35697161Test_seq4069476061M4I86M=407226430TCCAGGGACCTCAGCTATTCCATGAGATAATTTTACAAATGGTAGCCAGTGTCCTGTTTCAGATCGATCACAAATGTCCCTGTGGCCCCACTAGTGTCTTAAACATTATATAGGAACAAGGCTTTGCAGGGCAAAGACAGCAGTCCACTAGFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFF:F:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:4MD:Z:147MC:Z:151MAS:i:137XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1106:27236:3569781Test_seq40722660151M=406947-430GTGCAGCTTGTCCTTGCTCTTCAACTTTGTGTTGAGAAGTAAATAATAAGCTTGAATGCTCTTTTGCAGATAATCCTAACAGGGGAACCAACACTATAGCTAGAGATTCAGGAGAATTTGAAAAGAGGTTTACTCATTTGGAAATGGGAGAF,FFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFNM:i:0MD:Z:151MC:Z:61M4I86MAS:i:151XS:i:0Yt:Z:UU 60 60 TCCAGGGACCTCAGCTATTCCATGAGATAATTTTACAAATGGTAGCCAGTGTCCTGTTTCAGATCGATCACAAATGTCCCTGTGGCCCCACTAGTGTCTTAAACATTATATAGGAACAAGGCTTTGCAGGGCAAAGACAGCAGTCCACTAG GTGCAGCTTGTCCTTGCTCTTCAACTTTGTGTTGAGAAGTAAATAATAAGCTTGAATGCTCTTTTGCAGATAATCCTAACAGGGGAACCAACACTATAGCTAGAGATTCAGGAGAATTTGAAAAGAGGTTTACTCATTTGGAAATGGGAGA +A00126:224:H7HMWDSX3:3:1106:29008:36886 Test_seq 167972 Test_seq 168330 + - UU A00126:224:H7HMWDSX3:3:1106:29008:36886161Test_seq16797237151M=168180359TCGGAAGTGCGTTAAATGTATAGATGGCATTTGGTAGAATAGACATTTTTACTATGTTAAGTCTTCCTATCCATGAGCAAGGTATGTTTTTCCACTTAAGTATGTCCTTTTGAATTTCTTGTAGTAGAGCTTTGTAGTTTTCTTTCTATAGFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,F:FFFF:FF::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:10MD:Z:1T12T13T50G8C35C4T3A11G1G3MC:Z:151MAS:i:108XS:i:86Yt:Z:UU A00126:224:H7HMWDSX3:3:1106:29008:3688681Test_seq16818042151M=167972-359TGTGAATGGTATTGATTTGGTTATTTCCTCTTCGGTGTTCTTTTTGTTGATGTAGAGGAATCCAAGTGATTTTTGTATGTTTATTTTATAACCTGAGACTCTTCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGGFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFF:F:FFFF:F:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFNM:i:1MD:Z:91T59MC:Z:151MAS:i:146XS:i:126XA:Z:Test_seq,+212708,151M,5;Yt:Z:UU 37 42 TCGGAAGTGCGTTAAATGTATAGATGGCATTTGGTAGAATAGACATTTTTACTATGTTAAGTCTTCCTATCCATGAGCAAGGTATGTTTTTCCACTTAAGTATGTCCTTTTGAATTTCTTGTAGTAGAGCTTTGTAGTTTTCTTTCTATAG TGTGAATGGTATTGATTTGGTTATTTCCTCTTCGGTGTTCTTTTTGTTGATGTAGAGGAATCCAAGTGATTTTTGTATGTTTATTTTATAACCTGAGACTCTTCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGG +A00126:224:H7HMWDSX3:3:1107:20654:1313 Test_seq 382625 Test_seq 435035 - - UR A00126:224:H7HMWDSX3:3:1107:20654:13132209Test_seq4346256026H125M=434885411TAATAATTGCTTCTTCATGTTAAGGCTACAGCCATTCTGGTGTGTCTTGTTTTCATTCCCTTTTCCCCAAAGGATGCTGACCTGTTGGATGTTGAGAGCAAACACTTTGAAGACCTAGAATTCCAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFNM:i:0MD:Z:125MC:Z:151MAS:i:125XS:i:0SA:Z:Test_seq,382600,-,125S26M,35,1;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1107:20654:1313177Test_seq38260035125S26M=43488552411TGGAATTCTAGGTCTTCAAAGTGTTTGCTCTCAACATCCAACAGGTCAGCATCCTTTGGGGAAAAGGGAATGAAAACAAGACACACCAGAATGGCTGTAGCCTTAACATGAAGAAGCAATTATTATAATATGACTATTATTCAAATTTACTFFFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:25A0MC:Z:151MAS:i:25XS:i:0SA:Z:Test_seq,434625,+,26S125M,60,0;Yt:Z:UR A00126:224:H7HMWDSX3:3:1107:20654:1313113Test_seq43488560151M=382600-52411AAAAAAAAAAAAATTAAAATACACTCCTTGTATGTGACAGTAAAGATGGTCAGTATAGCTTTAACGTGTTATCTGTATGTCTGCATGTCTTTGTACCTGAACACCTTTGTGTATTTAATCACATATGGTATTTGGCAGACTGTGGATTCTGFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:125S26MAS:i:151XS:i:0Yt:Z:UR 35 60 TGGAATTCTAGGTCTTCAAAGTGTTTGCTCTCAACATCCAACAGGTCAGCATCCTTTGGGGAAAAGGGAATGAAAACAAGACACACCAGAATGGCTGTAGCCTTAACATGAAGAAGCAATTATTATAATATGACTATTATTCAAATTTACT AAAAAAAAAAAAATTAAAATACACTCCTTGTATGTGACAGTAAAGATGGTCAGTATAGCTTTAACGTGTTATCTGTATGTCTGCATGTCTTTGTACCTGAACACCTTTGTGTATTTAATCACATATGGTATTTGGCAGACTGTGGATTCTG +A00126:224:H7HMWDSX3:3:1107:4788:1892 Test_seq 166087 Test_seq 166508 + - UU A00126:224:H7HMWDSX3:3:1107:4788:189297Test_seq16608760151M=166358422GGAGTTGTCTGTTGTGTTCCCTGACAGGGCAGACATCGATTGTGGCCGGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCTTAGTTGTCGTGTGGCCTTGGTGTTCTTFFFFFFFFF:F:FFFF:FFF:,FFFF:FFF,FFFFFFFFFFFFFFFFFFFF,FF,FFFF::FFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF,FFFFFFFFFF:FFFF,FFFFFFFFFFFF,F:FFFFFFFFNM:i:1MD:Z:16C134MC:Z:151MAS:i:146XS:i:74Yt:Z:UU A00126:224:H7HMWDSX3:3:1107:4788:1892145Test_seq16635860151M=166087-422ATTATTTTGCCCGTTGACTTAGAAGTCTCCTCAAACCATGTTCCCCAGACCCCAGCCCCTGCTCCGCTGACCTTTGAAGCTTTCATTTTATCCCGGAAACCTCTTTGCTTTTAGTCCAGTCCAATTAGGCTGACCTTCCTTGTATTGTGTG,FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:43Yt:Z:UU 60 60 GGAGTTGTCTGTTGTGTTCCCTGACAGGGCAGACATCGATTGTGGCCGGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCTTAGTTGTCGTGTGGCCTTGGTGTTCTT ATTATTTTGCCCGTTGACTTAGAAGTCTCCTCAAACCATGTTCCCCAGACCCCAGCCCCTGCTCCGCTGACCTTTGAAGCTTTCATTTTATCCCGGAAACCTCTTTGCTTTTAGTCCAGTCCAATTAGGCTGACCTTCCTTGTATTGTGTG +A00126:224:H7HMWDSX3:3:1107:7120:2801 Test_seq 165764 Test_seq 166210 + - UU A00126:224:H7HMWDSX3:3:1107:7120:280197Test_seq1657646016S135M=166060447ACTTGCTCTCCCCCTATTCAGTCTCTTGTTTCGTGCCAATTTTGCCATCTTCCCTCTCTCTCTATCTTCCCATCCCCCCTCCAGTCAAGAGTTGCCAACACACTCTCCAGTGTCCACCTGATTTAATTAGCTCACTCTTCATCAGCATCTCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:4MD:Z:10C25T66A8G22MC:Z:151MAS:i:115XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1107:7120:2801145Test_seq16606060151M=165764-447GTATCCCATTGGTCTCCTGCTCCCTCAGGAGTTGTCTGTTGTGCTCCCTGACAGGGCAGACATCGATTGTGGCCGGGCACCAACTAGTTCTTCTGGTCTCAGGATAAGGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCTFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:107T43MC:Z:16S135MAS:i:146XS:i:75Yt:Z:UU 60 60 ACTTGCTCTCCCCCTATTCAGTCTCTTGTTTCGTGCCAATTTTGCCATCTTCCCTCTCTCTCTATCTTCCCATCCCCCCTCCAGTCAAGAGTTGCCAACACACTCTCCAGTGTCCACCTGATTTAATTAGCTCACTCTTCATCAGCATCTC GTATCCCATTGGTCTCCTGCTCCCTCAGGAGTTGTCTGTTGTGCTCCCTGACAGGGCAGACATCGATTGTGGCCGGGCACCAACTAGTTCTTCTGGTCTCAGGATAAGGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCT +A00126:224:H7HMWDSX3:3:1107:29279:4225 Test_seq 167906 Test_seq 168348 + - UU A00126:224:H7HMWDSX3:3:1107:29279:422597Test_seq16790660151M=168198443TTCCCTTCCATATGAAATTAGTGATTTGTTTCTCTATCCCCTTAAAATATGACATTGGTATTTGGATTGGAAGTGCGTTATATGTATAGATGGCTTTTGGTAGAATAGACATTTTTACTATGTTAAGTCTTCCTATCCATGAGCAGGGTATFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:41Yt:Z:UU A00126:224:H7HMWDSX3:3:1107:29279:4225145Test_seq16819839151M=167906-443GGTTATTTCCTCTTCGATGTTCTTTTTGTTGATGTAGAGGAATCCAAGTGATTTTTGTATGTTTATTTTATAACCTGAGACTCTTCCAAACTTTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGGTTTTCCATGTATACGATC:FFFFFFFFFFFFFFF,FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFFFFFFFFFFFNM:i:4MD:Z:16G56T18C55G2MC:Z:151MAS:i:133XS:i:113XA:Z:Test_seq,+212690,151M,8;Yt:Z:UU 60 39 TTCCCTTCCATATGAAATTAGTGATTTGTTTCTCTATCCCCTTAAAATATGACATTGGTATTTGGATTGGAAGTGCGTTATATGTATAGATGGCTTTTGGTAGAATAGACATTTTTACTATGTTAAGTCTTCCTATCCATGAGCAGGGTAT GGTTATTTCCTCTTCGATGTTCTTTTTGTTGATGTAGAGGAATCCAAGTGATTTTTGTATGTTTATTTTATAACCTGAGACTCTTCCAAACTTTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGGTTTTCCATGTATACGATC +A00126:224:H7HMWDSX3:3:1107:9968:5729 ! 0 Test_seq 442019 - - NR A00126:224:H7HMWDSX3:3:1107:9968:572997Test_seq4416456031S120M=441869375TTCCACAGGATTTTCAAAGAGCAGATGGATTGATCATTCAGTTTCATTCTTTCCCTTCATTACTCCAAAATCTATTCTCTAAATACCCTTTTCCATAATAGCAAATATTTCTTGATTTCTATTCCTTTGTGGAGATGCTTGCCATTTACATFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFF:FFFFFFFFFF:F,FFFFFFF:F:FFFFFF::F,FFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:120MC:Z:151MAS:i:120XS:i:0Yt:Z:NR A00126:224:H7HMWDSX3:3:1107:9968:5729145Test_seq44186960151M=441645-375TGTTTTACTAAAATAAATATTCTCTCGAGTAGGTTGATAGTGACTTTGGTCTTTGAATCACTTATGTGTTTTAAAAACGATGTACATATTTTATAATTTGTTTAACAGATTTACACATTCAGTATTTTGGGTAATTAAGTAAAATACATGAFFFFFFFFFFFFFF:,FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:31S120MAS:i:151XS:i:0Yt:Z:NR 0 60 TGTTTTACTAAAATAAATATTCTCTCGAGTAGGTTGATAGTGACTTTGGTCTTTGAATCACTTATGTGTTTTAAAAACGATGTACATATTTTATAATTTGTTTAACAGATTTACACATTCAGTATTTTGGGTAATTAAGTAAAATACATGA +A00126:224:H7HMWDSX3:3:1107:1597:6825 Test_seq 213742 Test_seq 214052 + - UU A00126:224:H7HMWDSX3:3:1107:1597:6825161Test_seq2137421919S132M=213902311GTAATGGTGCTCTGGGATTAAAACTCAACCACAAAAAGACAAACAACCCAATGAAGAAGTGGGCAAAGGATATGAACACGCACTTCACTAAAGAAGATATTCAGGCAACTAACAGATATATGAGAAAATGCTCTCGATCATTAACCATTATFFFFFFFF,FFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:,FFFFFF:FFFFFFFF:FF,:FFFFFFFFFFFFFFFFFFFFF:F,FFFF:FFFFF:FFFF:FFFFFFFFFFFF,:FF:FF:FFFFF:F:FF:FF:F::FFFF,NM:i:7MD:Z:17G6G8C54G10C24G6G0MC:Z:151MAS:i:101XS:i:91XA:Z:Test_seq,-167161,132M19S,9;Yt:Z:UU A00126:224:H7HMWDSX3:3:1107:1597:682581Test_seq21390252151M=213742-311TTCCATCTCACTCCAACAAGGCTGGCATTAATCCAAAAAACACAAAATAATAAATGTTGGAGAGGCTGCGGAGAGATTGGAACTCTTATACACTGCTGGTGGGAATGTAAAATGGTACAACCACTTTGGAAATCTATCTGGTGTTTTCTTA,FFFF,FFFFFFFFFFF:F,:F:,:FFFFFF:FFFFFFFFFFF,F:F,FFFF,FF:FFFFFFF,FFFFFFFFFFFFFFF,FFF:FFFFFFFFFFFFFFF:F,FFFFFFFFFF:FFFF:FFFFFF:FFFFFFFFFFFF:FFFF:FFFFFFFFNM:i:1MD:Z:76C74MC:Z:19S132MAS:i:146XS:i:121XA:Z:Test_seq,+400403,151M,6;Yt:Z:UU 19 52 GTAATGGTGCTCTGGGATTAAAACTCAACCACAAAAAGACAAACAACCCAATGAAGAAGTGGGCAAAGGATATGAACACGCACTTCACTAAAGAAGATATTCAGGCAACTAACAGATATATGAGAAAATGCTCTCGATCATTAACCATTAT TTCCATCTCACTCCAACAAGGCTGGCATTAATCCAAAAAACACAAAATAATAAATGTTGGAGAGGCTGCGGAGAGATTGGAACTCTTATACACTGCTGGTGGGAATGTAAAATGGTACAACCACTTTGGAAATCTATCTGGTGTTTTCTTA +A00126:224:H7HMWDSX3:3:1107:3414:7122 ! 0 ! 0 - - MM A00126:224:H7HMWDSX3:3:1107:3414:712265Test_seq140816052M=384324243509TCCTCTTCACTTTCAGCAAGCAAGGTTGTGTCATCTGCATAATGCAGGTTGTFFFFFFFF:FFFFFFFFFF::FFF:FF::FFFFFFFFFFFFFFFFFFFF:FFNM:i:1MD:Z:32T19MC:Z:52MAS:i:47XS:i:47XA:Z:Test_seq,+333187,52M,1;Test_seq,-384324,52M,1;Test_seq,-366380,50M2S,1;Test_seq,-17092,42M10S,0;Yt:Z:MM A00126:224:H7HMWDSX3:3:1107:3414:7122129Test_seq384324052M=140816-243509ACAACCTGTGTTATGCAGGTGACACAATCTTGCTTGCTGAAAGTGAAGAGGAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFNM:i:3MD:Z:8C9A8C24MC:Z:52MAS:i:37XS:i:37XA:Z:Test_seq,-333187,52M,3;Test_seq,-315912,52M,4;Yt:Z:MM 0 0 TCCTCTTCACTTTCAGCAAGCAAGGTTGTGTCATCTGCATAATGCAGGTTGT ACAACCTGTGTTATGCAGGTGACACAATCTTGCTTGCTGAAAGTGAAGAGGA +A00126:224:H7HMWDSX3:3:1107:30897:7185 Test_seq 171468 Test_seq 171865 + - UU A00126:224:H7HMWDSX3:3:1107:30897:7185161Test_seq17146860151M=171715398GTAGGGTTTCTTCATTTTTTTAAATTCTTTTATCTGATTTTTTTTCAGCTACGTTGGAGTTGATTCCCTGGTCCTCCAGAAGTCCCAGTCTACATTCTAATTGCTCGAGTCTGCTCCTCTGACTTTCTATTGCGATGTCTAATTCTGTAAT,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:FFFFFFFFF:FFFFFFFFF,FFFFFFFFFFFFFFFFNM:i:5MD:Z:0T20T29T63T18T16MC:Z:151MAS:i:130XS:i:41Yt:Z:UU A00126:224:H7HMWDSX3:3:1107:30897:718581Test_seq17171560151M=171468-398TTTTTCAACAGTTTTATCAGTGTGTTCCTTGGCTTTTTCTGCATTTATCCTAATTTCATTTGTGATATCTTTAAGCATTCTGTAAATTAGTTTTTTATATTCTGTATCTGATAATTCCAGGATTGTATCTTCATTTGGGAAAGATTTTGATFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:43G107MC:Z:151MAS:i:146XS:i:29Yt:Z:UU 60 60 GTAGGGTTTCTTCATTTTTTTAAATTCTTTTATCTGATTTTTTTTCAGCTACGTTGGAGTTGATTCCCTGGTCCTCCAGAAGTCCCAGTCTACATTCTAATTGCTCGAGTCTGCTCCTCTGACTTTCTATTGCGATGTCTAATTCTGTAAT TTTTTCAACAGTTTTATCAGTGTGTTCCTTGGCTTTTTCTGCATTTATCCTAATTTCATTTGTGATATCTTTAAGCATTCTGTAAATTAGTTTTTTATATTCTGTATCTGATAATTCCAGGATTGTATCTTCATTTGGGAAAGATTTTGAT +A00126:224:H7HMWDSX3:3:1107:20374:7247 Test_seq 167160 Test_seq 167535 + - UU A00126:224:H7HMWDSX3:3:1107:20374:724797Test_seq16716052151M=167385376TCTAATGGCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAATCATGTAGATTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFF:,FFFFFF:FFFF,FF,FFF:FFF:F:F:FFFF,FFFFFFF:FFFFFFF:NM:i:1MD:Z:83T67MC:Z:151MAS:i:146XS:i:121XA:Z:Test_seq,-213724,151M,6;Yt:Z:UU A00126:224:H7HMWDSX3:3:1107:20374:7247145Test_seq16738560151M=167160-376TTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCGGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATACCTTGTATTAGGGCTCCTAGGGTTGTCCCAATTTTTTCTTCCATGATCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFNM:i:6MD:Z:57T44G2C22A0T2T18MC:Z:151MAS:i:121XS:i:72Yt:Z:UU 52 60 TCTAATGGCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAATCATGTAGATT TTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCGGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATACCTTGTATTAGGGCTCCTAGGGTTGTCCCAATTTTTTCTTCCATGATC +A00126:224:H7HMWDSX3:3:1107:8594:7232 Test_seq 39918 Test_seq 168865 - - UU A00126:224:H7HMWDSX3:3:1107:8594:7232113Test_seq39851668M=168798128948CTATATCCCTCATGCCCTTAGATGCAAAAATCCTCAATAAAATTCTAGCCAATAGAATTCAACAGCATFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFNM:i:5MD:Z:14A0A1A19C26A3MC:Z:68MAS:i:44XS:i:39XA:Z:Test_seq,+153099,68M,6;Test_seq,+168802,4S64M,5;Yt:Z:UU A00126:224:H7HMWDSX3:3:1107:8594:7232177Test_seq1687981468M=39851-128948GTGTTGTTGGATTCTATTGGCTAGAATTTTGTTGAGGATTTTTGCATCAATGTTCATGAGGGATATAGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:48T19MC:Z:68MAS:i:63XS:i:57XA:Z:Test_seq,+39851,68M,3;Yt:Z:UU 6 14 CTATATCCCTCATGCCCTTAGATGCAAAAATCCTCAATAAAATTCTAGCCAATAGAATTCAACAGCAT GTGTTGTTGGATTCTATTGGCTAGAATTTTGTTGAGGATTTTTGCATCAATGTTCATGAGGGATATAG +A00126:224:H7HMWDSX3:3:1107:20500:7529 Test_seq 167160 Test_seq 167535 + - UU A00126:224:H7HMWDSX3:3:1107:20500:752997Test_seq16716052151M=167385376TCTAATGGCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAATCATGTAGATTFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF,FF:FF:FFFFFFF,FF:,FFFFF,FFFFF,F,FFF:F:FF,:,:FFFFFFFF:,:FFF,,,:F:FF,:,FNM:i:1MD:Z:83T67MC:Z:151MAS:i:146XS:i:121XA:Z:Test_seq,-213724,151M,6;Yt:Z:UU A00126:224:H7HMWDSX3:3:1107:20500:7529145Test_seq16738560151M=167160-376TTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTTTCGGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATACCTTGTATTAGGGCTCCTAGGGTTGTCCCAATTTTTTCTTCCATGATCFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF,FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF,FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFNM:i:7MD:Z:54A2T44G2C22A0T2T18MC:Z:151MAS:i:116XS:i:72Yt:Z:UU 52 60 TCTAATGGCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAATCATGTAGATT TTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTTTCGGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATACCTTGTATTAGGGCTCCTAGGGTTGTCCCAATTTTTTCTTCCATGATC +A00126:224:H7HMWDSX3:3:1107:4743:11271 Test_seq 171530 Test_seq 171892 + - UU A00126:224:H7HMWDSX3:3:1107:4743:1127197Test_seq17153060151M=171742363ATTCCCTGGTCCTACAGAAGTCCCAGTCTACATTCTAATTGCTTGAGTCTGCTCCTCTGACTTTCTCTTGCATTGTCAAATTCTGTAATTTTATTGTTAATCTTTTGGATTTCTACATGCTGTCTCTCTATGGATTCTTGCAACTTATTAAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF,FFFFFF:FFFFFF,F::F:FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFF:F:F:F,FFFFNM:i:6MD:Z:13C29C9T12A4G5T73MC:Z:151MAS:i:121XS:i:59Yt:Z:UU A00126:224:H7HMWDSX3:3:1107:4743:11271145Test_seq17174260151M=171530-363CTTGGCTTTTTCTGCATTTATCCTAATTTCATTTGTGATATCTTTAAGCATTCTGTAAATTAGTTTTTTATATTCTGTATCTGATAATTCCAGGATTGTATCTTCATTTGGGAAAGATTTTGATTCTTTTGTTTGGGGGGTTGGAGAAGCTF,:F:F:FFF:F:FFFFF:FFFFFFFFFFFF:FFFFFFF:FFFFF,:FFFFFFFFFFFFFFFFF:F,FFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:16G134MC:Z:151MAS:i:146XS:i:25Yt:Z:UU 60 60 ATTCCCTGGTCCTACAGAAGTCCCAGTCTACATTCTAATTGCTTGAGTCTGCTCCTCTGACTTTCTCTTGCATTGTCAAATTCTGTAATTTTATTGTTAATCTTTTGGATTTCTACATGCTGTCTCTCTATGGATTCTTGCAACTTATTAA CTTGGCTTTTTCTGCATTTATCCTAATTTCATTTGTGATATCTTTAAGCATTCTGTAAATTAGTTTTTTATATTCTGTATCTGATAATTCCAGGATTGTATCTTCATTTGGGAAAGATTTTGATTCTTTTGTTTGGGGGGTTGGAGAAGCT +A00126:224:H7HMWDSX3:3:1107:15727:13573 ! 0 Test_seq 170940 - - NR A00126:224:H7HMWDSX3:3:1107:15727:1357397Test_seq1705846026S125M=170790357GCTTCAGGTGACAATCAAAATAGATTAGTCGTTACGTTTTTCTTGAGTTATGGAATTGATATTCCTTTTTGTGGTTACCTTATTATTTACCCCTATTTTTCTAAGTAAAAACCTAACTTGTATCGTTCTATATCGCCTTGTATCACTCTCCFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFF,FF,F:FFFFF,FFFFFF:FFFF::FFFFFFF,F::FF,FF,FF,FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF,FFFFFFF:FFFFF:FFF:FFFFFFFFFNM:i:2MD:Z:101G6A16MC:Z:151MAS:i:115XS:i:37Yt:Z:NR A00126:224:H7HMWDSX3:3:1107:15727:13573145Test_seq17079060151M=170584-357TGTTATATGTATTATTTTGTTTATTTATTTATTTTTTAGAATTAATCTTACTTTGTTTGTTTTTGTGCTTTCCCTATTTGAGTTGCGTTGATATCAGGACGTTCTGTTTTGTGACCTTGTATTGTGCTGGTACCTGATATTATTGGTCATCFFF:,F,FFFFFFFFFF:FFF:FFFFFFFFFFFFFF:FFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFNM:i:1MD:Z:6G144MC:Z:26S125MAS:i:146XS:i:0Yt:Z:NR 0 60 TGTTATATGTATTATTTTGTTTATTTATTTATTTTTTAGAATTAATCTTACTTTGTTTGTTTTTGTGCTTTCCCTATTTGAGTTGCGTTGATATCAGGACGTTCTGTTTTGTGACCTTGTATTGTGCTGGTACCTGATATTATTGGTCATC +A00126:224:H7HMWDSX3:3:1107:5719:14184 Test_seq 165873 Test_seq 166278 + - UU A00126:224:H7HMWDSX3:3:1107:5719:1418497Test_seq1658736029M2D122M=166128406ATTGGCTCACTCTTCATCAGCATCTCTCTCCCCCCCGCTGACCAGTCCTTTTCATGCCTGATGAGTTGTCTTCGGGGATGGTTCCTGTCCTGTGCCATCAGAAGTTCTGGGGAGCATTGTCTCTGGGATTCCTCTAGTCGCATTCATATCAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFF:FF:FFFFFFFF:FF:FFFF:FFFFFF,FFFFFF,FNM:i:4MD:Z:29^CC27T82A11MC:Z:151MAS:i:133XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1107:5719:14184145Test_seq16612860151M=165873-406GTGGCCGGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCTTAGTTGTCGTGTGGCCTTGGTGTTCTTCCTTTGCCTTTGCTCCAGGTGGGTTGAGACCAATTGATGTAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:29M2D122MAS:i:151XS:i:87Yt:Z:UU 60 60 ATTGGCTCACTCTTCATCAGCATCTCTCTCCCCCCCGCTGACCAGTCCTTTTCATGCCTGATGAGTTGTCTTCGGGGATGGTTCCTGTCCTGTGCCATCAGAAGTTCTGGGGAGCATTGTCTCTGGGATTCCTCTAGTCGCATTCATATCA GTGGCCGGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCTTAGTTGTCGTGTGGCCTTGGTGTTCTTCCTTTGCCTTTGCTCCAGGTGGGTTGAGACCAATTGATGTA +A00126:224:H7HMWDSX3:3:1107:11984:14857 ! 0 Test_seq 168630 - - NR A00126:224:H7HMWDSX3:3:1107:11984:14857161Test_seq1683476025S126M=168480284GACCTTTTTCTAGGCAATAGATCGATCATGTCATCTGCAAATAGTGATAACTTTACTTCTTCCTTGCCAATCCGGATACCCTTTATTTCTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCCAGTACGATGTTGAATAAGAGCGGTGATAFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FNM:i:5MD:Z:18A5G9C12T49A28MC:Z:151MAS:i:101XS:i:68Yt:Z:NR A00126:224:H7HMWDSX3:3:1107:11984:1485781Test_seq16848060151M=168347-284TCCTTGTCTGGTTCCTGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATCGGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:148G2MC:Z:25S126MAS:i:148XS:i:84Yt:Z:NR 0 60 TCCTTGTCTGGTTCCTGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATCGG +A00126:224:H7HMWDSX3:3:1107:5376:14904 Test_seq 168347 Test_seq 168675 + - UU A00126:224:H7HMWDSX3:3:1107:5376:1490497Test_seq1683476016S135M=168525329ATGACTAGTGCTTAGATCATGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCTTGCCAATCTGGATACCCTTTATTTCTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFNM:i:1MD:Z:18A116MC:Z:151MAS:i:130XS:i:73Yt:Z:UU A00126:224:H7HMWDSX3:3:1107:5376:14904145Test_seq16852560151M=168347-329TCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTGTGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATCFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFNM:i:1MD:Z:49A101MC:Z:16S135MAS:i:146XS:i:82Yt:Z:UU 60 60 ATGACTAGTGCTTAGATCATGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCTTGCCAATCTGGATACCCTTTATTTCTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATC TCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTGTGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATC +A00126:224:H7HMWDSX3:3:1107:5737:15029 Test_seq 170350 Test_seq 170652 + - UU A00126:224:H7HMWDSX3:3:1107:5737:1502997Test_seq17035060151M=170514303GATGGATCGTGTTTCTTTATCCAGTCCGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTTTTCATGTGTGTTGTTGGCCATTTCATTTTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F,F:FF:F:FFNM:i:1MD:Z:140T10MC:Z:84M12I55MAS:i:146XS:i:48Yt:Z:UU A00126:224:H7HMWDSX3:3:1107:5737:15029145Test_seq1705146084M12I55M=170350-303TGTGCTGAGACGTTTTTCTTAGTAGCTTGTGAGGTCCTCATTTTCATAATGTTTAACTTTATGTTTGTTGAGTCATTACGTTTTTCTTGGCTTTTCTCTTGAGTTATGAAATTGATATTCCTTTTTGTGGTTACCTTATTATTTACCCCTGFFFFF:FFFFFF,FFFFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:17MD:Z:25A7A40G21G41A0MC:Z:151MAS:i:100XS:i:27Yt:Z:UU 60 60 GATGGATCGTGTTTCTTTATCCAGTCCGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTTTTCATGTGTGTTGTTGGCCATTTCATTTTT TGTGCTGAGACGTTTTTCTTAGTAGCTTGTGAGGTCCTCATTTTCATAATGTTTAACTTTATGTTTGTTGAGTCATTACGTTTTTCTTGGCTTTTCTCTTGAGTTATGAAATTGATATTCCTTTTTGTGGTTACCTTATTATTTACCCCTG +A00126:224:H7HMWDSX3:3:1107:21911:15264 Test_seq 98654 Test_seq 99058 + - UU A00126:224:H7HMWDSX3:3:1107:21911:15264161Test_seq9865460151M=98908405GATCCATCTGTCTTGTTTATCAATGCTTATATTGCTAGCCCTCCTGCTGGAGTTTTAATTCTAGCAGATCAAGAAAACTTTTCGCTAAGGGATCTTTTTAAGTCCATCTCAGTCAGGGAGAAGCCTTCTCTTTCGCCTTTCCACCTTCACCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF,FF,FFFFFFFFFFFFFFFFFFFFFFFFF,FFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1107:21911:1526481Test_seq9890860151M=98654-405CAAGGAGGGAAGGTCCAGCAGAGCAGGCTTCCAGAGGTGGGCCAGGTGCTTGTCACGGTCCTAAAATAGGGTTGGACGAAACAGGGTATTTGCTTCAGATGGGCCAGTAGTATGGCACCCCTTCAAGCTGTCACTTTAAAGACATTACTCAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU 60 60 GATCCATCTGTCTTGTTTATCAATGCTTATATTGCTAGCCCTCCTGCTGGAGTTTTAATTCTAGCAGATCAAGAAAACTTTTCGCTAAGGGATCTTTTTAAGTCCATCTCAGTCAGGGAGAAGCCTTCTCTTTCGCCTTTCCACCTTCACC CAAGGAGGGAAGGTCCAGCAGAGCAGGCTTCCAGAGGTGGGCCAGGTGCTTGTCACGGTCCTAAAATAGGGTTGGACGAAACAGGGTATTTGCTTCAGATGGGCCAGTAGTATGGCACCCCTTCAAGCTGTCACTTTAAAGACATTACTCA +A00126:224:H7HMWDSX3:3:1107:19768:19758 ! 0 Test_seq 168616 - - MU A00126:224:H7HMWDSX3:3:1107:19768:19758113Test_seq2127300151M=168466-44265CTACTGAAACTAATAGAAGAGTTTGGCAGAGACTCAGGTTATAAAATAAACATACAAAAATCACTTGGATTCCTCTACATCAACAAAAAGAACACTGAAGAGGAAATAACCAAATCAATACCGTTCACAGTAGCCCCCAAGAAGATAAGATFFFF,FFFFFFFFFFF,FFFFFFFFFFFFFF,F:FF:,FFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFF:FFFFFF:FFFFFFF:FFFF,FFFNM:i:7MD:Z:31T12G49T0C0A25A25A2MC:Z:151MAS:i:118XS:i:118XA:Z:Test_seq,+168158,151M,7;Yt:Z:MU A00126:224:H7HMWDSX3:3:1107:19768:19758177Test_seq16846660151M=21273044265GGTGATAAAGGGCATCCTTGTCTGGTTCCTGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:86Yt:Z:MU 0 60 CTACTGAAACTAATAGAAGAGTTTGGCAGAGACTCAGGTTATAAAATAAACATACAAAAATCACTTGGATTCCTCTACATCAACAAAAAGAACACTGAAGAGGAAATAACCAAATCAATACCGTTCACAGTAGCCCCCAAGAAGATAAGAT GGTGATAAAGGGCATCCTTGTCTGGTTCCTGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTT +A00126:224:H7HMWDSX3:3:1107:22318:21010 Test_seq 109072 Test_seq 109396 + - UU A00126:224:H7HMWDSX3:3:1107:22318:2101097Test_seq10907260151M=109250325ACTCAGAAAAAGAGCGTGAGAATGGTTGCACAACTCATCGGTGTCACTGAATCGTACATGTAGAAACTGTTGAATTGGCGTATATTCTGCCGTTTATATTCTTAAAAACAACAACAAATAAACTGTTGTTTAAAAAAAAATACCGTCAAGAFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF,FFFFFFFF,,FFFFF:FFF,FFF,,FFFFFFFFFFFFF:FF,F::FFFFF:FNM:i:0MD:Z:151MC:Z:33M4I114MAS:i:151XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1107:22318:21010145Test_seq1092506033M4I114M=109072-325AGAAAATATTTTTAATACGTATATTTGACAAAGGATCGATCTGTATCCAGACTACATTAAGAACTCAATAAAAAATTTTTAAACTTTCAGTTTTAAAAATGGGCATGAGTAGACATTTCACAGAAGAAGACATAAAACGTCATATTAAAAGFFFFF:F,FFFFFFFFFFFFFF::FFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFNM:i:4MD:Z:147MC:Z:151MAS:i:137XS:i:0Yt:Z:UU 60 60 ACTCAGAAAAAGAGCGTGAGAATGGTTGCACAACTCATCGGTGTCACTGAATCGTACATGTAGAAACTGTTGAATTGGCGTATATTCTGCCGTTTATATTCTTAAAAACAACAACAAATAAACTGTTGTTTAAAAAAAAATACCGTCAAGA AGAAAATATTTTTAATACGTATATTTGACAAAGGATCGATCTGTATCCAGACTACATTAAGAACTCAATAAAAAATTTTTAAACTTTCAGTTTTAAAAATGGGCATGAGTAGACATTTCACAGAAGAAGACATAAAACGTCATATTAAAAG +A00126:224:H7HMWDSX3:3:1107:23764:21230 ! 0 Test_seq 190124 - - NR A00126:224:H7HMWDSX3:3:1107:23764:2123097Test_seq1896736029S122M=189974452AAGTCCTTACCTACTCTCTCCAGCTCTGAGATCTCATCAGCGAGTAGCTTGATTCCCCGTTTAGGGAACAGCTACCTACTAGACATTTCTAGACTCTTCCTTGCTGGGAAATATCACTAATCTGGGAGACTTACCACAATGCAGTATTATTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF::FFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFF:F:FFF,F,FFFFFFFF:FFF:F,FFFFFFFFFFFFFFF:FFFFFFF:,FFFFFF,FNM:i:0MD:Z:122MC:Z:151MAS:i:122XS:i:0Yt:Z:NR A00126:224:H7HMWDSX3:3:1107:23764:21230145Test_seq18997460151M=189673-452TGCAGCAAGATATTAATATTTTAAGAGAAAATGAAATTAGAAACAAAACCTCATTCATTTGAGTGCAAAGGCTAACACAAATGGAGAATAAAAGAAATATAAAGCATTGCCCCTCCTCTCAAGAAGACTACCGTAACTTCGCAGGTACCGT,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FF:FFF,FF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFF:FFFFFFFFF:FFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:29S122MAS:i:151XS:i:0Yt:Z:NR 0 60 TGCAGCAAGATATTAATATTTTAAGAGAAAATGAAATTAGAAACAAAACCTCATTCATTTGAGTGCAAAGGCTAACACAAATGGAGAATAAAAGAAATATAAAGCATTGCCCCTCCTCTCAAGAAGACTACCGTAACTTCGCAGGTACCGT +A00126:224:H7HMWDSX3:3:1107:24921:24361 Test_seq 168347 Test_seq 168675 + - UU A00126:224:H7HMWDSX3:3:1107:24921:2436197Test_seq1683476017S134M=168525329TTCCACAGACGGATTGATCATGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCTTGCCAATCTGGATACCCTTTATTTCTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGTACGATGGTGAATAAGAGTGGTGATAAAGGGCATFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFNM:i:3MD:Z:18A88T10C15MC:Z:151MAS:i:119XS:i:66Yt:Z:UU A00126:224:H7HMWDSX3:3:1107:24921:24361145Test_seq16852560151M=168347-329TCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATCFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:17S134MAS:i:151XS:i:0Yt:Z:UU 60 60 TTCCACAGACGGATTGATCATGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCTTGCCAATCTGGATACCCTTTATTTCTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGTACGATGGTGAATAAGAGTGGTGATAAAGGGCAT TCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATC +A00126:224:H7HMWDSX3:3:1107:23222:25551 Test_seq 171161 Test_seq 171577 + - UU A00126:224:H7HMWDSX3:3:1107:23222:25551161Test_seq17116160151M=171427417CTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATGGGGTTCGATGAGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFF:FF:FFFFFF:FFFFFFFFF:FF:FFFFFF:FFFFFFFFFFFFFFFFFFFFF,FFFFFFFFF:FFFF,FFFF::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:35Yt:Z:UU A00126:224:H7HMWDSX3:3:1107:23222:2555181Test_seq17142760151M=171161-417CACTCGCAAGTTATCCTTCTTGATAGAGCCCCACATGATTCTTAGGGTTTCTTCATTTTTTTTAATTCTTTTATCTGATTTTTTTTCAGCTATGTTGGTGTTGATTCCCTGGTCCTTCAGATGTCCCAGTCTACATTCTAATTGCTCGAGTFFFFFFFFFFFFFFFF:,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:4MD:Z:28T69A17C4A29MC:Z:151MAS:i:131XS:i:42Yt:Z:UU 60 60 CTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATGGGGTTCGATGAG CACTCGCAAGTTATCCTTCTTGATAGAGCCCCACATGATTCTTAGGGTTTCTTCATTTTTTTTAATTCTTTTATCTGATTTTTTTTCAGCTATGTTGGTGTTGATTCCCTGGTCCTTCAGATGTCCCAGTCTACATTCTAATTGCTCGAGT +A00126:224:H7HMWDSX3:3:1107:5014:25895 ! 0 Test_seq 168741 - - NR A00126:224:H7HMWDSX3:3:1107:5014:2589597Test_seq1683476023S128M=168591395TTGGGCCCTGGCACCTGGATCGATCATGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCTTGCCAATCCGAATACCCTTTATTTCTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAFFFFFFFFFFF,F:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:3MD:Z:18A28T1G78MC:Z:151MAS:i:113XS:i:65Yt:Z:NR A00126:224:H7HMWDSX3:3:1107:5014:25895145Test_seq16859160151M=168347-395TTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAAC:FFFF:FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFF:FF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:125T25MC:Z:23S128MAS:i:146XS:i:39Yt:Z:NR 0 60 TTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAAC +A00126:224:H7HMWDSX3:3:1107:24108:26459 ! 0 Test_seq 167465 - + NR A00126:224:H7HMWDSX3:3:1107:24108:2645981Test_seq1676146124M27S=167465-273TGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATTATTTCCCCAATTGACTGACACTGGTCCTTTGTCAAATATCAGCTGCTCATACGTGGATGGATCGATCACTTATCCTATACTGGACCAGC:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:3MD:Z:61C50T1A9MC:Z:151MAS:i:109XS:i:104XA:Z:Test_seq,+40970,27S124M,4;Test_seq,+213301,27S124M,4;Yt:Z:NR A00126:224:H7HMWDSX3:3:1107:24108:26459161Test_seq16746560151M=167614273TAATGTTTTGTATTCTGTTTATGCCCTGTATTAGGGCTCCTAGGGTTGATCCTATTTTTTCTTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFNM:i:0MD:Z:151MC:Z:124M27SAS:i:151XS:i:106Yt:Z:NR 0 60 TAATGTTTTGTATTCTGTTTATGCCCTGTATTAGGGCTCCTAGGGTTGATCCTATTTTTTCTTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTG +A00126:224:H7HMWDSX3:3:1107:14281:27383 Test_seq 171469 Test_seq 171884 + - UU A00126:224:H7HMWDSX3:3:1107:14281:2738397Test_seq17146960151M=171734416TAGGGTTTCTTCATTTTTTTTAATTCTTTTATCTGATTTTTTTTCAGCTATGTTGGTGTTGATTCCCTGGTCCTCCAGAAGTCCCAGTCTACATTCTAATTGCTCGAGTCTGCTCCTCTGACTTCTTATTGCATTGTCTAATTCTGTAATTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFF:FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFNM:i:5MD:Z:56A57T9T0C6G18MC:Z:151MAS:i:126XS:i:38Yt:Z:UU A00126:224:H7HMWDSX3:3:1107:14281:27383145Test_seq17173460151M=171469-416GTGTGTTCCTTGGCTTTTTCTGCAGTTACCCTAATTTCATTTGTGATATCTTTAAGCATTCTGTAAATTAGTTTTTTATATTCTGTATCTGATAATTCCAGGATTGTATCTTCATTTGGGAAAGATTTTGATTCTTTTGTTTGGGGGGTTGFFFFF:FFFF,FFFFFF,FFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFF:FFFFFFF,FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:28T122MC:Z:151MAS:i:146XS:i:25Yt:Z:UU 60 60 TAGGGTTTCTTCATTTTTTTTAATTCTTTTATCTGATTTTTTTTCAGCTATGTTGGTGTTGATTCCCTGGTCCTCCAGAAGTCCCAGTCTACATTCTAATTGCTCGAGTCTGCTCCTCTGACTTCTTATTGCATTGTCTAATTCTGTAATT GTGTGTTCCTTGGCTTTTTCTGCAGTTACCCTAATTTCATTTGTGATATCTTTAAGCATTCTGTAAATTAGTTTTTTATATTCTGTATCTGATAATTCCAGGATTGTATCTTCATTTGGGAAAGATTTTGATTCTTTTGTTTGGGGGGTTG +A00126:224:H7HMWDSX3:3:1107:24252:27868 Test_seq 167149 Test_seq 167601 + - UU A00126:224:H7HMWDSX3:3:1107:24252:27868161Test_seq16714942151M=167455453ATTTGCATTTCTCTAATGGCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFF:FFFFFFFFFFFFFNM:i:1MD:Z:94T56MC:Z:113M4I34MAS:i:146XS:i:126XA:Z:Test_seq,-213735,151M,5;Yt:Z:UU A00126:224:H7HMWDSX3:3:1107:24252:2786881Test_seq1674556113M4I34M=167149-453TCATTTTTGGTAATGTTTTGTATTCTGTTTATACCTTGTATTAGGGCTCCTAGGGTTGTCCCAATTTTTTCTTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFF:FFFFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFNM:i:9MD:Z:32G2C22A0T2T84MC:Z:151MAS:i:112XS:i:107XA:Z:Test_seq,-400918,113M4I34M,10;Yt:Z:UU 42 6 ATTTGCATTTCTCTAATGGCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAA TCATTTTTGGTAATGTTTTGTATTCTGTTTATACCTTGTATTAGGGCTCCTAGGGTTGTCCCAATTTTTTCTTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTG +A00126:224:H7HMWDSX3:3:1107:3025:29340 Test_seq 260193 Test_seq 260507 + - UU A00126:224:H7HMWDSX3:3:1107:3025:2934097Test_seq26019360151M=260357315AGTGATTTGCCCAAGACCACTCAGTTATAATTACATAATTGTAGAGGAATTCCAAATTTTTTATTTAAAAGAAGAAGCAGGAAACATTCTAAATCTGAAAGTATTGTAATTTTTCAAGTAATTTTTTAAAATGCCATTTGCAAATATCAAGFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF,FFFFFFFFFFF:FFF:FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1107:3025:29340145Test_seq26035760151M=260193-315GGAAATGAGACTTGTTTTAGGTGCCATCAAGCTGATTCTGACTCCTAGTGAGGCTATGTAAAACAGACCAAAACACTGTTCCTGTGCCATCCTCACAATTGTTATGCTTGAGCCCATTGTTGCAGCGACTGTGTCAGTCCATCTTGTTGAG,:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF,FFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FNM:i:2MD:Z:67A41A41MC:Z:151MAS:i:141XS:i:31Yt:Z:UU 60 60 AGTGATTTGCCCAAGACCACTCAGTTATAATTACATAATTGTAGAGGAATTCCAAATTTTTTATTTAAAAGAAGAAGCAGGAAACATTCTAAATCTGAAAGTATTGTAATTTTTCAAGTAATTTTTTAAAATGCCATTTGCAAATATCAAG GGAAATGAGACTTGTTTTAGGTGCCATCAAGCTGATTCTGACTCCTAGTGAGGCTATGTAAAACAGACCAAAACACTGTTCCTGTGCCATCCTCACAATTGTTATGCTTGAGCCCATTGTTGCAGCGACTGTGTCAGTCCATCTTGTTGAG +A00126:224:H7HMWDSX3:3:1107:24695:29888 Test_seq 169081 Test_seq 169467 + - UU A00126:224:H7HMWDSX3:3:1107:24695:29888161Test_seq16908160151M=169317387GTTTCAATCTCTTTTTTTGTTATGGGTCTATTTAGTTGTTCTGCTTCTGAATGTGTTAGTTTAGGTAGGTAGTGTTTTTCAAGGAATTCATCCATTTCTTCTAGGTTTTCAAATTTGTTAGAGTACAATTTTTCATAATAATCTGAAATGAFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFF:FFFFFFF:FF:FFFFFFFFF:F:FFF,F,FF:FFFF:FFFF:F:,FFFFF:FFFFFFFF:F,FFFF:FF:,:FFFFF::NM:i:7MD:Z:42A37G1A37C11A1G2G13MC:Z:151MAS:i:116XS:i:75Yt:Z:UU A00126:224:H7HMWDSX3:3:1107:24695:2988881Test_seq16931760151M=169081-387AGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGAFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:150G0MC:Z:151MAS:i:150XS:i:112Yt:Z:UU 60 60 GTTTCAATCTCTTTTTTTGTTATGGGTCTATTTAGTTGTTCTGCTTCTGAATGTGTTAGTTTAGGTAGGTAGTGTTTTTCAAGGAATTCATCCATTTCTTCTAGGTTTTCAAATTTGTTAGAGTACAATTTTTCATAATAATCTGAAATGA AGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGA +A00126:224:H7HMWDSX3:3:1107:1759:30718 Test_seq 169163 Test_seq 169463 + - UU A00126:224:H7HMWDSX3:3:1107:1759:3071897Test_seq16916360151M=169313301GGAATTCATCCATTTCTTCTAGGTTTTCAAATTTGTTAGAGTACAATTTTTCATAATAATCTGAAATGATTCTTTTAATTTCATTTGGCTCTGTTGTGATGTGGTCCTTCTCATTTCTTATTCGGGTTATTTGTTTCCTTTCCTGTATTTCFFFFFFFFFF:F:FF:FF,FFFFFFFFFFFFFFFFFFF,FF:FFFFFFFFFFF:FFF:FFFF:FFFF,FFFFFFFFFFFF:,FFFF,:,F:FFFF:F:FFFFFFF::F:FF,FFFFFFF,FF,,FFFFFFFFFFFF,,FFF:,F:FFF:FFNM:i:8MD:Z:0A37C11A1G2G49T6G33T4MC:Z:151MAS:i:115XS:i:73Yt:Z:UU A00126:224:H7HMWDSX3:3:1107:1759:30718145Test_seq16931360151M=169163-301CTTTAGTCAGTCTAGCCAATGTTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGAF,FFF:FFF,FFFF:F,F:FF,FFFF:,F,F:FFFFFF:FFFFF:,FFFFFFFFF:FFFFF:FF,FF,:FFFFFF:F:FFFFF,:FF:FFFFFFFFFFFFFF:F::FFFFFFFFF:FFFF:F:F:FFFFFFFFFFFFFFF:FF,FF:FFFFNM:i:1MD:Z:21G129MC:Z:151MAS:i:146XS:i:107Yt:Z:UU 60 60 GGAATTCATCCATTTCTTCTAGGTTTTCAAATTTGTTAGAGTACAATTTTTCATAATAATCTGAAATGATTCTTTTAATTTCATTTGGCTCTGTTGTGATGTGGTCCTTCTCATTTCTTATTCGGGTTATTTGTTTCCTTTCCTGTATTTC CTTTAGTCAGTCTAGCCAATGTTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGA +A00126:224:H7HMWDSX3:3:1107:10936:32894 Test_seq 168278 Test_seq 168664 + - UU A00126:224:H7HMWDSX3:3:1107:10936:3289497Test_seq16827844151M=168514387CTCTGCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGGTTTTCCATGTATACGACTATGTCATCTGCAAATACGGATAGCTTTACTTCCTCCTTGCCAATCCGGATACCCTTTATTTCTTTGTCTAGCCTAATTGTFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFFF,F:F:FFFFFFF:FFF:FFFF:FFFFNM:i:8MD:Z:4T63G0T0C16A0T27T33C0MC:Z:151MAS:i:115XS:i:90Yt:Z:UU A00126:224:H7HMWDSX3:3:1107:10936:32894145Test_seq16851460151M=168278-387TTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTAFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:89Yt:Z:UU 44 60 CTCTGCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGGTTTTCCATGTATACGACTATGTCATCTGCAAATACGGATAGCTTTACTTCCTCCTTGCCAATCCGGATACCCTTTATTTCTTTGTCTAGCCTAATTGT TTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTA +A00126:224:H7HMWDSX3:3:1107:5629:33536 Test_seq 168347 Test_seq 168652 + - UU A00126:224:H7HMWDSX3:3:1107:5629:3353697Test_seq1683476018S133M=168502306CCATCAAACACAGATCGATCATGTCATCTGCAAGTAGTGATAGCTTTACTTCCTCCTTGCCAATCGGGATACCCTTTATTTCTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGCAGTGATAAAGGGCAFFFFFFFFFFF,FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:4MD:Z:15A2A28T71G13MC:Z:151MAS:i:113XS:i:70Yt:Z:UU A00126:224:H7HMWDSX3:3:1107:5629:33536145Test_seq16850260151M=168347-306AAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATCGGTGTTGAACTTTGTCAAATGCCT,FFF,FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:126G24MC:Z:18S133MAS:i:146XS:i:78Yt:Z:UU 60 60 CCATCAAACACAGATCGATCATGTCATCTGCAAGTAGTGATAGCTTTACTTCCTCCTTGCCAATCGGGATACCCTTTATTTCTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGCAGTGATAAAGGGCA AAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATCGGTGTTGAACTTTGTCAAATGCCT +A00126:224:H7HMWDSX3:3:1107:4390:33865 Test_seq 359511 Test_seq 359937 + - UU A00126:224:H7HMWDSX3:3:1107:4390:3386597Test_seq35951160151M=359787427TGGCAGAGCACAGCCAAATGCAAAAGCGGCTGGATTTCCAAAATGGTAGCTTAGAGGAAGGTCTTGAAGCAAATTCCCTTGAGAGCAATCCCCTAAACGTGATGGAGAGCCTAAGCCAGAAGAAATACTCTTCCAGTCTGAGATTTAAAGCFFFFFFFFFFFFFFFFFFFFF:F,FFFFFFFFFF:F:FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1107:4390:33865145Test_seq35978760151M=359511-427AGTTCTCTGGTGATGGCACTGACAGAAATCTTTCCACGAAGCCTCCCACTCCTTCAGTCAGCACCTCACCCTCCCTCGGTGGTTATCTGCTTGGCAGAGTGGAATTGGATCATTATACTGGTCGGGACAGTGAAAGGTCCTTGAGGGTCTCFFFFFFF:FFFFF::FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU 60 60 TGGCAGAGCACAGCCAAATGCAAAAGCGGCTGGATTTCCAAAATGGTAGCTTAGAGGAAGGTCTTGAAGCAAATTCCCTTGAGAGCAATCCCCTAAACGTGATGGAGAGCCTAAGCCAGAAGAAATACTCTTCCAGTCTGAGATTTAAAGC AGTTCTCTGGTGATGGCACTGACAGAAATCTTTCCACGAAGCCTCCCACTCCTTCAGTCAGCACCTCACCCTCCCTCGGTGGTTATCTGCTTGGCAGAGTGGAATTGGATCATTATACTGGTCGGGACAGTGAAAGGTCCTTGAGGGTCTC +A00126:224:H7HMWDSX3:3:1107:32389:34538 Test_seq 168968 Test_seq 169451 + - UU A00126:224:H7HMWDSX3:3:1107:32389:3453897Test_seq16896860151M=169301484TTTGAAATACCTTCAGTAGTAGTGGTGTTAAGTCTTCTCTGAAGGTTTGGTAGAACTCTTCAGTGAAGCCGTCCGGGCCAGGACTGTTTTTTGTTGGAAGTTTTTTGATTACCGTTTCAATCTCTTTTTTTGTTATGGGTCTATTTAGTTG:FFFFFFFFFFFFFFF::F:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FF:FFFFFFFFFFFFFFFFF:FFF:FFFFFFF:FFFFFFFF:F:F:F::FF:FFFF:FF,F,FFNM:i:3MD:Z:59G13T11T65MC:Z:151MAS:i:136XS:i:71Yt:Z:UU A00126:224:H7HMWDSX3:3:1107:32389:34538145Test_seq16930160151M=168968-484TTTCCTGTATTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:8T142MC:Z:151MAS:i:146XS:i:107Yt:Z:UU 60 60 TTTGAAATACCTTCAGTAGTAGTGGTGTTAAGTCTTCTCTGAAGGTTTGGTAGAACTCTTCAGTGAAGCCGTCCGGGCCAGGACTGTTTTTTGTTGGAAGTTTTTTGATTACCGTTTCAATCTCTTTTTTTGTTATGGGTCTATTTAGTTG TTTCCTGTATTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCT +A00126:224:H7HMWDSX3:3:1107:29170:34663 Test_seq 167300 Test_seq 214051 - - UU A00126:224:H7HMWDSX3:3:1107:29170:34663113Test_seq16715042151M=21390146752TTTGCATTTCTCTAATGGCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAATFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:93T57MC:Z:151MAS:i:146XS:i:126XA:Z:Test_seq,+213734,151M,5;Yt:Z:UU A00126:224:H7HMWDSX3:3:1107:29170:34663177Test_seq2139018151M=167150-46752ATTCCATCTCACACCAGCAAGGCTGGCATTAATCCAAAAAACACAAAATAATAAATGTTGGAGAGGCTGCGGAGAGATTGGAACTCTCATACACTGCTGGTGGGAATGTAAAATGGTACAACCACTTTGGAAATCTATCTGGCGTTATCTTFFF,FFF:FFFFFFF::FFFFFFFFFFFFF,:FFFFF:FFFF:FFF:FF::,FFFF::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:6MD:Z:12T3A60C9T54T3T4MC:Z:151MAS:i:122XS:i:116XA:Z:Test_seq,+166983,151M,7;Test_seq,+400413,9S142M,8;Yt:Z:UU 42 8 TTTGCATTTCTCTAATGGCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAAT ATTCCATCTCACACCAGCAAGGCTGGCATTAATCCAAAAAACACAAAATAATAAATGTTGGAGAGGCTGCGGAGAGATTGGAACTCTCATACACTGCTGGTGGGAATGTAAAATGGTACAACCACTTTGGAAATCTATCTGGCGTTATCTT +A00126:224:H7HMWDSX3:3:1107:14027:34741 Test_seq 170044 Test_seq 170439 + - UU A00126:224:H7HMWDSX3:3:1107:14027:34741161Test_seq17004460151M=170289396GAGGTGTCTATCTTGCTTTTCAATTCTGTTAAAATTTGATTTGTGTATCTTGCAGCCCTGTCATTGGGTGCGTAAATATTTAATATGGTTATATCTTCCTGATCAATTGTCCCTTTTATCATTATATAGTGTCCTTCTTTATCCTTTGTGGFFFFFFFFFF:FFFFFFF:FFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFF:F:,FFFFFFFF,F,FFF,F:FFF:F,FFF:FFFFF:,,FFFFNM:i:4MD:Z:3A9C28A20C87MC:Z:151MAS:i:132XS:i:82Yt:Z:UU A00126:224:H7HMWDSX3:3:1107:14027:3474181Test_seq17028960151M=170044-396TTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:84Yt:Z:UU 60 60 GAGGTGTCTATCTTGCTTTTCAATTCTGTTAAAATTTGATTTGTGTATCTTGCAGCCCTGTCATTGGGTGCGTAAATATTTAATATGGTTATATCTTCCTGATCAATTGTCCCTTTTATCATTATATAGTGTCCTTCTTTATCCTTTGTGG TTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAA +A00126:224:H7HMWDSX3:3:1107:5041:36213 Test_seq 167566 Test_seq 167973 + - UU A00126:224:H7HMWDSX3:3:1107:5041:3621397Test_seq1675662812S139M=167823408GAGCTAGGCTTGTTGATCCATTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATCATTTCCCCAATTGACTGACACTGGTCCTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFNM:i:1MD:Z:8C130MC:Z:151MAS:i:134XS:i:121XA:Z:Test_seq,-213334,126M25S,1;Yt:Z:UU A00126:224:H7HMWDSX3:3:1107:5041:36213145Test_seq16782360151M=167566-408ATAATAGGTTCTGAAATCAGGTAGAGTGAGGCCTCCCACTTTCTTCTTCTTTTTCAGTAATGCTTTGCTTATCCGGGTGTTCTTTCCCTTCCATATGAAATTAGTGATTTGTTTCTCTATCCCCTTAAAATATGACATTGGTATTTGGATTFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFNM:i:1MD:Z:77G73MC:Z:12S139MAS:i:146XS:i:90Yt:Z:UU 28 60 GAGCTAGGCTTGTTGATCCATTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATCATTTCCCCAATTGACTGACACTGGTCCTT ATAATAGGTTCTGAAATCAGGTAGAGTGAGGCCTCCCACTTTCTTCTTCTTTTTCAGTAATGCTTTGCTTATCCGGGTGTTCTTTCCCTTCCATATGAAATTAGTGATTTGTTTCTCTATCCCCTTAAAATATGACATTGGTATTTGGATT +A00126:224:H7HMWDSX3:3:1108:25816:1767 Test_seq 170144 Test_seq 170533 + - UU A00126:224:H7HMWDSX3:3:1108:25816:176797Test_seq17014460151M=170383390GATCAATTGTCCCTTTTATCATTATGTAGTGTCCTTCTTTATCCTTTGTGGTGGATTTAAGTCTAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCCTCTTCTTTTTTGCTTACTGTTTGCTTGATATATTTTTTTCCATCCTTTGAGFFFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FF,FFF:FFFFFFFNM:i:5MD:Z:25A25C0A63T1A32MC:Z:151MAS:i:126XS:i:54Yt:Z:UU A00126:224:H7HMWDSX3:3:1108:25816:1767145Test_seq17038360151M=170144-390CTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTTTTCATGTGTGTTGTTGGCCGTTTCATTTTTCCACATACTTTTTTGTGCTGAGACGTTTTTCTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:107T43MC:Z:151MAS:i:146XS:i:45Yt:Z:UU 60 60 GATCAATTGTCCCTTTTATCATTATGTAGTGTCCTTCTTTATCCTTTGTGGTGGATTTAAGTCTAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCCTCTTCTTTTTTGCTTACTGTTTGCTTGATATATTTTTTTCCATCCTTTGAG CTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTTTTCATGTGTGTTGTTGGCCGTTTCATTTTTCCACATACTTTTTTGTGCTGAGACGTTTTTCTT +A00126:224:H7HMWDSX3:3:1108:6497:1939 Test_seq 168461 Test_seq 168808 + - UU A00126:224:H7HMWDSX3:3:1108:6497:193997Test_seq16846160151M=168658348AGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:,,F,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:,FF:FFFFFFFFFFFFFFFFF::FFFFFFF,FFFFFFFF,FFFNM:i:1MD:Z:34T116MC:Z:151MAS:i:146XS:i:91Yt:Z:UU A00126:224:H7HMWDSX3:3:1108:6497:1939145Test_seq16865860151M=168461-348GCATCAATTGATAAGATCATGTGGTTTTTGTCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGAFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:3MD:Z:5T23A28T92MC:Z:151MAS:i:136XS:i:60Yt:Z:UU 60 60 AGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAG GCATCAATTGATAAGATCATGTGGTTTTTGTCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGA +A00126:224:H7HMWDSX3:3:1108:9634:5588 Test_seq 40166 Test_seq 212883 + - UU A00126:224:H7HMWDSX3:3:1108:9634:5588161Test_seq40166179M3I69M=212733172718CAACAGCCAACATCACCCTAAATGGGGAGAGACTGAAAGCATTCCCCTTGAGAACGGGAACCAGACAAGGATGCCTTTTATCACCACTCGTATTCAACACTGTGCTAGAGGTCCTAGCCAGAGCAATTAGGCTAGGCAAAGAAATAAAGGGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFF:FFFFFFNM:i:16MD:Z:15T9A5C11T21G9C5T0G3T9T6G28A0T14MC:Z:151MAS:i:74XS:i:71XA:Z:Test_seq,+212488,151M,16;Test_seq,-168400,151M,17;Test_seq,-50359,64M13I74M,24;Yt:Z:UU A00126:224:H7HMWDSX3:3:1108:9634:558881Test_seq21273360151M=40166-172718CTGAAACTAATAGAAGAGTTTGGCAGAGTCTCAGGTTATAAGATAAACATACAAAAATCACTTGGATTCCTCTACATCAACAAAAAGAACATCAAAGAGGAAATCACCAAATCAATACCATTCACAGTAGCCCCCAAGAAGATAAAATACTF,F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:104A46MC:Z:79M3I69MAS:i:146XS:i:116Yt:Z:UU 1 60 CAACAGCCAACATCACCCTAAATGGGGAGAGACTGAAAGCATTCCCCTTGAGAACGGGAACCAGACAAGGATGCCTTTTATCACCACTCGTATTCAACACTGTGCTAGAGGTCCTAGCCAGAGCAATTAGGCTAGGCAAAGAAATAAAGGG CTGAAACTAATAGAAGAGTTTGGCAGAGTCTCAGGTTATAAGATAAACATACAAAAATCACTTGGATTCCTCTACATCAACAAAAAGAACATCAAAGAGGAAATCACCAAATCAATACCATTCACAGTAGCCCCCAAGAAGATAAAATACT +A00126:224:H7HMWDSX3:3:1108:16071:6872 Test_seq 36103 Test_seq 167548 + + UR A00126:224:H7HMWDSX3:3:1108:16071:6872129Test_seq361031026M125S=167548131446TGCAAAGGCAATTCTGATAGGGATCTAAGGGAATAGAGAAACAAATCACTAATTTCATATGGAAAGGAAAGAACCCCCGGATAAGCAAAACATTACTGAAAAAGAAGAAGAAAGTGGGAGGCCTCACTCTACCTGATTTCAGAACCTATTCFFFF:FFFF:::FFFFFFFFFF::F,,FFFFF,::F:FF::FF,FFFFFF,FFFFFF,FFFF,,FFFF,FFF::FFFFFF:F,,FFF::FF:F::FFFF,F:FFF:F,:FFF,F:FFFFFFFFF:FF,F,,F:FF,:,FFFFF,FFF,F:,NM:i:1MD:Z:20T5MC:Z:151MAS:i:21XS:i:0SA:Z:Test_seq,167824,-,126M25S,60,4;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1108:16071:68722193Test_seq16782460126M25H=167548-402GAATAGGTTCTGAAATCAGGTAGAGTGAGGCCTCCCACTTTCTTCTTCTTTTTCAGTAATGTTTTGCTTATCCGGGGGTTCTTTCCTTTCCATATGAAATTAGTGATTTGTTTCTCTATTCCCTTA,:F,FFF,FFFFF,:,FF:F,,F,FF:FFFFFFFFF:F,FFF:,F:FFF:F,FFFF::F:FF::FFF,,F:FFFFFF::FFF,FFFF,,FFFF,FFFFFF,FFFFFF,FF::FF:F::,FFFFF,,NM:i:4MD:Z:0T60C24C32C6MC:Z:151MAS:i:110XS:i:75SA:Z:Test_seq,36103,+,26M125S,10,1;Yt:Z:UR A00126:224:H7HMWDSX3:3:1108:16071:687265Test_seq16754842151M=36103-131446GTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATTATTTCCCCAATTGACTGACACTGF:F,FFFFFFF::FFFFFFFF:F:,:FFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFF,FFF:F::FFFFF:FFF:FFFFF::,FFFF,F:F:::FFF,FF,::F:F:F,:FFFFFF:F:FF::F,FF:FFF:::,,,FFF::FFF:::F:NM:i:1MD:Z:127C23MC:Z:26M125SAS:i:146XS:i:126XA:Z:Test_seq,-213340,120M2I24M5S,4;Yt:Z:UR 10 42 TGCAAAGGCAATTCTGATAGGGATCTAAGGGAATAGAGAAACAAATCACTAATTTCATATGGAAAGGAAAGAACCCCCGGATAAGCAAAACATTACTGAAAAAGAAGAAGAAAGTGGGAGGCCTCACTCTACCTGATTTCAGAACCTATTC GTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATTATTTCCCCAATTGACTGACACTG +A00126:224:H7HMWDSX3:3:1108:7292:7576 Test_seq 167298 Test_seq 167624 + - UU A00126:224:H7HMWDSX3:3:1108:7292:7576161Test_seq1672982119S132M=167474327ATGAGGGTAATGATGATTAAATCATGTAGATTTTAGAGATCAGGCGCTGGTCTGAGATGTCATAGCTGAATATTCTTTCCCAGTCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFF:F,FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFNM:i:2MD:Z:25A44A61MC:Z:151MAS:i:122XS:i:112XA:Z:Test_seq,-213605,132M19S,4;Test_seq,+400719,19S132M,6;Yt:Z:UU A00126:224:H7HMWDSX3:3:1108:7292:757681Test_seq16747460151M=167298-327GTATTCTGTTTATGCCCTGTATTAGGGCTCCTAGGGTTGATCCTATTTTTTCTTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:19S132MAS:i:151XS:i:104Yt:Z:UU 21 60 ATGAGGGTAATGATGATTAAATCATGTAGATTTTAGAGATCAGGCGCTGGTCTGAGATGTCATAGCTGAATATTCTTTCCCAGTCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAG GTATTCTGTTTATGCCCTGTATTAGGGCTCCTAGGGTTGATCCTATTTTTTCTTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCT +A00126:224:H7HMWDSX3:3:1108:24126:7701 Test_seq 55744 Test_seq 65171 + - RU A00126:224:H7HMWDSX3:3:1108:24126:770197Test_seq5574460151M=651449428TCTCAACAGAGAAGCTGCCAAAACACTGAAAATCTTGGCCTGGCCAATTTGACACACAATCTTAACCATCACAAATAGTTCGTCTTCTTGAGCAGTCCTTTGAAATCTTCTGTTCAGCTCTTTTACTTAATCATTTCTTCCTTTCAATTTAFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFNM:i:0MD:Z:151MC:Z:123S28MAS:i:151XS:i:0Yt:Z:RU A00126:224:H7HMWDSX3:3:1108:24126:77012193Test_seq5601560123M28H=55744-394TTCCACACTCATCCGGTATTGGGTCATCAGTGTTCAATGCATGAAATTATTCTTGAGACAGTCTCTAAATTCAGGTGGGATATGCTCAACATTGTGTACTTTGGCTCTCGTGGATTTGTTTTAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:123MC:Z:151MAS:i:123XS:i:23SA:Z:Test_seq,65144,-,123S28M,54,0;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1108:24126:7701145Test_seq6514454123S28M=55744-9428TTCCACACTCATCCGGTATTGGGTCATCAGTGTTCAATGCATGAAATTATTCTTGAGACAGTCTCTAAATTCAGGTGGGATATGCTCAACATTGTGTACTTTGGCTCTCGTGGATTTGTTTTATAAGAACCAGAGGTCAGATGATGACGAGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:28MC:Z:151MAS:i:28XS:i:0SA:Z:Test_seq,56015,-,123M28S,60,0;Yt:Z:RU 60 54 TCTCAACAGAGAAGCTGCCAAAACACTGAAAATCTTGGCCTGGCCAATTTGACACACAATCTTAACCATCACAAATAGTTCGTCTTCTTGAGCAGTCCTTTGAAATCTTCTGTTCAGCTCTTTTACTTAATCATTTCTTCCTTTCAATTTA TTCCACACTCATCCGGTATTGGGTCATCAGTGTTCAATGCATGAAATTATTCTTGAGACAGTCTCTAAATTCAGGTGGGATATGCTCAACATTGTGTACTTTGGCTCTCGTGGATTTGTTTTATAAGAACCAGAGGTCAGATGATGACGAG +A00126:224:H7HMWDSX3:3:1108:21061:8187 Test_seq 168347 Test_seq 168776 + - UU A00126:224:H7HMWDSX3:3:1108:21061:8187161Test_seq1683476017S134M=168626430AAATGTTTGAACTTAGATCATGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCTTACCAATCTGGATACCCTTTATTTCTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF::FFFFFFFFFNM:i:2MD:Z:18A21G93MC:Z:151MAS:i:124XS:i:67Yt:Z:UU A00126:224:H7HMWDSX3:3:1108:21061:818781Test_seq16862660151M=168347-430ATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:90T60MC:Z:17S134MAS:i:146XS:i:72Yt:Z:UU 60 60 AAATGTTTGAACTTAGATCATGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCTTACCAATCTGGATACCCTTTATTTCTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCAT ATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATC +A00126:224:H7HMWDSX3:3:1108:18909:8469 Test_seq 104742 Test_seq 114432 + - UR A00126:224:H7HMWDSX3:3:1108:18909:846997Test_seq1047426031M120S=1142829691CACATATCAGGTGTCAACAGGGAAGTGATCAATCCACAAAACATCTCACAACGTGGATGAATCTGGAGGGCATTATGCTGAGTGAAATAAGTCATTCACAAAAAGACAAATAATGTGTGAGATCACTATTATAAGAACTCAAGAAAAGGTTFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:31MC:Z:151MAS:i:31XS:i:0SA:Z:Test_seq,114039,+,30S121M,60,0;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1108:18909:84692145Test_seq1140396030H121M=114282394AATCCACAAAACATCTCACAACGTGGATGAATCTGGAGGGCATTATGCTGAGTGAAATAAGTCATTCACAAAAAGACAAATAATGTGTGAGATCACTATTATAAGAACTCAAGAAAAGGTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:121MC:Z:151MAS:i:121XS:i:73SA:Z:Test_seq,104742,+,31M120S,60,0;Yt:Z:UR A00126:224:H7HMWDSX3:3:1108:18909:8469145Test_seq11428260151M=104742-9691CAGCACAACTGGGCCAAAGCAAAACAAAGAAATTTCCTGGGCACATCTAAACACTGCAAGGGACAGAGTAGCTGGGGTTGGCGATTGGGGACCATAGTTTTGGGGAACATCTAGGTCAACTGCAATAATGAAGTTTATTAATAAAATGTTCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:81T69MC:Z:31M120SAS:i:146XS:i:0Yt:Z:UR 60 60 CACATATCAGGTGTCAACAGGGAAGTGATCAATCCACAAAACATCTCACAACGTGGATGAATCTGGAGGGCATTATGCTGAGTGAAATAAGTCATTCACAAAAAGACAAATAATGTGTGAGATCACTATTATAAGAACTCAAGAAAAGGTT CAGCACAACTGGGCCAAAGCAAAACAAAGAAATTTCCTGGGCACATCTAAACACTGCAAGGGACAGAGTAGCTGGGGTTGGCGATTGGGGACCATAGTTTTGGGGAACATCTAGGTCAACTGCAATAATGAAGTTTATTAATAAAATGTTC +A00126:224:H7HMWDSX3:3:1108:14498:8656 Test_seq 171559 Test_seq 171939 + - UU A00126:224:H7HMWDSX3:3:1108:14498:865697Test_seq17155960151M=171789381ACATTCTAATTGCTCAAGTCTGCTCCTCTGACTTTCTATTGCATTGTCAAATTCTGTAATTTTATTGTTAATCTTTTGGATTTCTACATGATGTCTCTCTACGGATTCTTGCAACTTATTAATTTTTCCACTATGTTCTTGAATAATCTTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF,FF:FFFFFFF,FFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:F:FFFFFF:FFFFFF,FFFFFFFFFFFFF,FFFF:FFFNM:i:8MD:Z:15G8T17G5T41C10T30C13C4MC:Z:151MAS:i:111XS:i:67Yt:Z:UU A00126:224:H7HMWDSX3:3:1108:14498:8656145Test_seq17178960151M=171559-381GCATTCTGTAAATTAGTTTTTTATATTCTGTATCTGATAATTCCAGGATTGTATCTTCATTTGGGAAAGATTTTGATTCTTTTGTTTGGGGGGTTGGAGAAGCTGTCATGGTCTGTTTCTTTATGTGGTTTGATATGGACTGCTGTCTCTGFFFFFFF:FFFFFF,FF:FFFFFF:FF::FFFFFFFFFFF::FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFNM:i:1MD:Z:149C1MC:Z:151MAS:i:149XS:i:0Yt:Z:UU 60 60 ACATTCTAATTGCTCAAGTCTGCTCCTCTGACTTTCTATTGCATTGTCAAATTCTGTAATTTTATTGTTAATCTTTTGGATTTCTACATGATGTCTCTCTACGGATTCTTGCAACTTATTAATTTTTCCACTATGTTCTTGAATAATCTTT GCATTCTGTAAATTAGTTTTTTATATTCTGTATCTGATAATTCCAGGATTGTATCTTCATTTGGGAAAGATTTTGATTCTTTTGTTTGGGGGGTTGGAGAAGCTGTCATGGTCTGTTTCTTTATGTGGTTTGATATGGACTGCTGTCTCTG +A00126:224:H7HMWDSX3:3:1108:10031:9471 ! 0 Test_seq 167492 - - NR A00126:224:H7HMWDSX3:3:1108:10031:947197Test_seq1671734130S121M=167342320ACTTGTATATCCAGTGTCTTGCTAGTGATCGATCGGGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGCTTGTCTGTTTGTGGTTGAGTTTTGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFF:FFFFFFFFFFFFFFFNM:i:4MD:Z:5A64T25T6T17MC:Z:151MAS:i:101XS:i:81XA:Z:Test_seq,-213741,121M30S,8;Yt:Z:NR A00126:224:H7HMWDSX3:3:1108:10031:9471145Test_seq16734221151M=167173-320AGCTGAATATTCTTTCCCAGTCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATGCCCTGFFF:FFFFFFFF,FFFFFFF:FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFFFFF:::FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:26A124MC:Z:30S121MAS:i:146XS:i:136XA:Z:Test_seq,+213542,151M,3;Yt:Z:NR 0 21 AGCTGAATATTCTTTCCCAGTCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATGCCCTG +A00126:224:H7HMWDSX3:3:1108:15148:12007 Test_seq 169307 Test_seq 169699 + - UU A00126:224:H7HMWDSX3:3:1108:15148:12007161Test_seq16930760151M=169549393GTTTTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCGGGTFFFFFFFFFFFFFF,FF:FFFFF::FFFFFFFF:FF:F,,FFFFFF:FFFFFFFFFFFFFFFF::FFFFF:FFFFFFFF:FFFFFFF,FFFFFF:FFF:FFFFF:FFFFFF:FFFF::F:FF:F,,FFFF::FF:FFFFFFFFF:FFFFFFNM:i:1MD:Z:147T3MC:Z:151MAS:i:147XS:i:103Yt:Z:UU A00126:224:H7HMWDSX3:3:1108:15148:1200781Test_seq16954960151M=169307-393GCATTTATCAATATAAATTGGCCTCTGAGCACTGCTTTTGCTGTGTCCCAGAGGTTTTGATAGGAAGTATTTTCATTCTCGTTGCTTTCTAAGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTATFFFFFF:FFFF:FFFFF:FFFFFFFF:FFFFFFFFFF::FF:F:F:F::F:F:FFFFFFF::FFFFFFFFF:FFF:FFFF:,FFFFFFFFFFFF::,FFF:FF,,FFFFFF,FFF:FFFFFFFFFF:FFFFFFFFFF:FFF,FF,FFF:FFNM:i:3MD:Z:9G69T34G36MC:Z:151MAS:i:136XS:i:28Yt:Z:UU 60 60 GTTTTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCGGGT GCATTTATCAATATAAATTGGCCTCTGAGCACTGCTTTTGCTGTGTCCCAGAGGTTTTGATAGGAAGTATTTTCATTCTCGTTGCTTTCTAAGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTAT +A00126:224:H7HMWDSX3:3:1108:8576:12962 Test_seq 169329 Test_seq 169811 + - UU A00126:224:H7HMWDSX3:3:1108:8576:1296297Test_seq16932960151M=169665483CAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGGTTCTTTTGTTGCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:NM:i:0MD:Z:151MC:Z:4S147MAS:i:151XS:i:111Yt:Z:UU A00126:224:H7HMWDSX3:3:1108:8576:12962145Test_seq169665604S147M=169329-483TGACGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTATTATTCATTTTCCAAGTATTTGATTTCTTTTCCCTAGTTTTTCTGTTATTGATTTCTAGTTTCATTGCCTTGTGGTCTGAGAAGATGCTTTGTAATATTTCGATGTTTTGGACFFFFFFF:F,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:4MD:Z:36G45C10C52T0MC:Z:151MAS:i:131XS:i:76Yt:Z:UU 60 60 CAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGGTTCTTTTGTTGC TGACGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTATTATTCATTTTCCAAGTATTTGATTTCTTTTCCCTAGTTTTTCTGTTATTGATTTCTAGTTTCATTGCCTTGTGGTCTGAGAAGATGCTTTGTAATATTTCGATGTTTTGGAC +A00126:224:H7HMWDSX3:3:1108:19253:12884 Test_seq 146453 Test_seq 146833 + - UU A00126:224:H7HMWDSX3:3:1108:19253:1288497Test_seq14645360151M=146683381CCTATTGGCTGACTGTAGATAACACACTGTTAGGTTCTGGGAGGCTGTTTACTACTTGAGAGCAGAGTGTTTTTGCAACACTCTATTGACCCAAAACACAAGGTTCCCAACGTTTAGGTAAGGAGCCCTGGTGGTGCAATGGTTAAGCACTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1108:19253:12884145Test_seq14668360151M=146453-381GAGATTACAACCTGGGAAATCCTGTGGGGCACGTTCTGCTCTATTATATGGGGCTGCTGTGACTCGGAATCTACCTGACATCACACAATAGTGCTGAGGTTAAATGCTTTATGTTTGGGGTGTACAGTTGTGGTAATTCTTCCTTCTGTGGFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU 60 60 CCTATTGGCTGACTGTAGATAACACACTGTTAGGTTCTGGGAGGCTGTTTACTACTTGAGAGCAGAGTGTTTTTGCAACACTCTATTGACCCAAAACACAAGGTTCCCAACGTTTAGGTAAGGAGCCCTGGTGGTGCAATGGTTAAGCACT GAGATTACAACCTGGGAAATCCTGTGGGGCACGTTCTGCTCTATTATATGGGGCTGCTGTGACTCGGAATCTACCTGACATCACACAATAGTGCTGAGGTTAAATGCTTTATGTTTGGGGTGTACAGTTGTGGTAATTCTTCCTTCTGTGG +A00126:224:H7HMWDSX3:3:1108:25455:13761 Test_seq 169282 Test_seq 169777 + - UU A00126:224:H7HMWDSX3:3:1108:25455:1376197Test_seq16928260151M=169627496ATTCGGGTTATTTGTTTCCTTTCCTGTTTTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:::FFFFFFFFFFFF:FFF:FFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:98Yt:Z:UU A00126:224:H7HMWDSX3:3:1108:25455:13761145Test_seq16962760151M=169282-496TCGTTGCTTTCTAAGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTATTGTTCATTTTCCAAGTATTTGATTTCTTTTCCCTAGTTTTTCTATTATTGATTTCTAGCTTCATTGCCTTGTGGTCTGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFF:F::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:4MD:Z:1T34G79G3C30MC:Z:151MAS:i:134XS:i:0Yt:Z:UU 60 60 ATTCGGGTTATTTGTTTCCTTTCCTGTTTTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAA TCGTTGCTTTCTAAGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTATTGTTCATTTTCCAAGTATTTGATTTCTTTTCCCTAGTTTTTCTATTATTGATTTCTAGCTTCATTGCCTTGTGGTCTG +A00126:224:H7HMWDSX3:3:1108:23674:14653 Test_seq 166058 Test_seq 166469 + - UU A00126:224:H7HMWDSX3:3:1108:23674:1465397Test_seq16605860151M=166319412CTGTATCCCATTGGTCTCCTGCTCCCTCAGGAGTTGTCTGTTGTGCTCCCTGACAGGGCAGACATCGATTGTGGCCGGGCACCAACTCGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFF,F:FFFF:FFFFFFFFFNM:i:1MD:Z:87A63MC:Z:151MAS:i:146XS:i:75Yt:Z:UU A00126:224:H7HMWDSX3:3:1108:23674:14653145Test_seq16631960151M=166058-412CACAATTCAAAGTGGGATGCAGAGTGTTTTCATAATAGAATTGTTTTGCCCATTGACTTAGAAGTCCCCTCAAACCAAGTTCCCCAGACCCCAGCCCCTGCTTCGTTGACCTTTGAAGCTTTCATTTTATCTCGGAAACCTCTTTACTTTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:9MD:Z:23A18A8G14T10T24C2C25C13G5MC:Z:151MAS:i:106XS:i:0Yt:Z:UU 60 60 CTGTATCCCATTGGTCTCCTGCTCCCTCAGGAGTTGTCTGTTGTGCTCCCTGACAGGGCAGACATCGATTGTGGCCGGGCACCAACTCGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTT CACAATTCAAAGTGGGATGCAGAGTGTTTTCATAATAGAATTGTTTTGCCCATTGACTTAGAAGTCCCCTCAAACCAAGTTCCCCAGACCCCAGCCCCTGCTTCGTTGACCTTTGAAGCTTTCATTTTATCTCGGAAACCTCTTTACTTTT +A00126:224:H7HMWDSX3:3:1108:21983:14794 ! 0 Test_seq 130883 - + NR A00126:224:H7HMWDSX3:3:1108:21983:14794145Test_seq13110160127M24S=130883-345AAATAAGTTATCTGTGTAAGACCAAATGAACAACAGCTATTTTAAAGCTAGACAGGATGGTTGAGGGGCAGGGAGTTTAAGTCAATGGAAGTCAAACAACTAGAACAGAAATAACAAGAATGTTGACAATCACAAGGTCCTAGAAATGTCC::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF,FFFFNM:i:1MD:Z:57A69MC:Z:151MAS:i:122XS:i:0Yt:Z:NR A00126:224:H7HMWDSX3:3:1108:21983:1479497Test_seq13088360151M=131101345GAGAGGGCAGAGGTGTCCAGAAGCTACCCAAATGGACACGAGGAGAGAGGGGGGAGGGAAGTACTGTGCTATTTGGTCAGAGGGAGAACAATTAGGAGTATATAGCAAGGTATATGTAAATTTTTGTATGAGAGACTGACCTGATTTGTAAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFNM:i:0MD:Z:151MC:Z:127M24SAS:i:151XS:i:39Yt:Z:NR 0 60 GAGAGGGCAGAGGTGTCCAGAAGCTACCCAAATGGACACGAGGAGAGAGGGGGGAGGGAAGTACTGTGCTATTTGGTCAGAGGGAGAACAATTAGGAGTATATAGCAAGGTATATGTAAATTTTTGTATGAGAGACTGACCTGATTTGTAA +A00126:224:H7HMWDSX3:3:1108:30110:17973 Test_seq 212594 Test_seq 212897 + - UU A00126:224:H7HMWDSX3:3:1108:30110:17973161Test_seq21259460151M=212747304AGAAGTCCTAGCCAGGGCAATTAGGCTAGACAAAGAAATAAAGGGCATCTGGATTGCCAAGGAGGAAGTAAAATTATCTCTATTTGCAGATGACATGATCTTATACACAGAAAACCCTAAGGAATTCTCCAGAAAACTACTGAAACTAATAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:5MD:Z:3G11A40G61T6C25MC:Z:151MAS:i:127XS:i:85Yt:Z:UU A00126:224:H7HMWDSX3:3:1108:30110:1797381Test_seq21274742151M=212594-304AGAGTTTGGCAGAGTCTCAGGTTATAAGATAAACATACAAAAATCACTTGGATTCCTCTACATCAACAAAAAGAACATCGAAGAGGAAATAACCAAATCAATACCATTCACAGTAGCCCCCAAGAAGATAAAATACTTAGGAATAAATCTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFNM:i:1MD:Z:79A71MC:Z:151MAS:i:146XS:i:126XA:Z:Test_seq,+168141,151M,5;Yt:Z:UU 60 42 AGAAGTCCTAGCCAGGGCAATTAGGCTAGACAAAGAAATAAAGGGCATCTGGATTGCCAAGGAGGAAGTAAAATTATCTCTATTTGCAGATGACATGATCTTATACACAGAAAACCCTAAGGAATTCTCCAGAAAACTACTGAAACTAATA AGAGTTTGGCAGAGTCTCAGGTTATAAGATAAACATACAAAAATCACTTGGATTCCTCTACATCAACAAAAAGAACATCGAAGAGGAAATAACCAAATCAATACCATTCACAGTAGCCCCCAAGAAGATAAAATACTTAGGAATAAATCTT +A00126:224:H7HMWDSX3:3:1108:7003:19069 Test_seq 168274 Test_seq 168713 + - UU A00126:224:H7HMWDSX3:3:1108:7003:1906997Test_seq16827460151M=168563440GAGACTCTTCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGGTTTTCCATGTATACGATCATGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCTTGCCAATCTGGACACCCTTTATTTCTTTGTCTAGCCTAAFFFFFFFFFFFF:,FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFF:FFFF:F:FFFFFFFFFNM:i:3MD:Z:72G18A32T26MC:Z:151MAS:i:136XS:i:91Yt:Z:UU A00126:224:H7HMWDSX3:3:1108:7003:19069145Test_seq16856360151M=168274-440TGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGCGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGATFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFF:FFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:66G84MC:Z:151MAS:i:146XS:i:76Yt:Z:UU 60 60 GAGACTCTTCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGGTTTTCCATGTATACGATCATGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCTTGCCAATCTGGACACCCTTTATTTCTTTGTCTAGCCTAA TGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGCGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGAT +A00126:224:H7HMWDSX3:3:1108:3179:19805 Test_seq 167317 Test_seq 213898 - - UU A00126:224:H7HMWDSX3:3:1108:3179:19805113Test_seq16716752151M=21376746582GCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAATCATGTAGATTTTAGAGAF:F:FFFFFFFFFF:FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFF:FFF:FF:FFFFF:FFFFFFFFFFFFFFFFFFNM:i:1MD:Z:76T74MC:Z:19S132MAS:i:146XS:i:121XA:Z:Test_seq,+213717,151M,6;Yt:Z:UU A00126:224:H7HMWDSX3:3:1108:3179:19805177Test_seq2137673519S132M=167167-46582AAAATTTAAAAAAAAAAACAACCCATTCAAGAAGTGGGCAAAGGATATGAACACGCACTTCACTAAAGAAGATATTCAGGCAGCTAACAGATACATGAGAAAATGCTCTTGATCATTAGCCATTAGAGAAATGCAAATTAAAACTATGATGFFFF,,,FFFFFFFFF:FFFFF,FF,F:FFFF:FF:F:FFFF:FFFFFFFFFFF,,F:F:FFFF:FFFFFFFFFFFFFF,FFFFFF:FFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFNM:i:4MD:Z:6A83C33G2C4MC:Z:151MAS:i:114XS:i:97XA:Z:Test_seq,+167136,136M15S,8;Yt:Z:UU 52 35 GCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAATCATGTAGATTTTAGAGA AAAATTTAAAAAAAAAAACAACCCATTCAAGAAGTGGGCAAAGGATATGAACACGCACTTCACTAAAGAAGATATTCAGGCAGCTAACAGATACATGAGAAAATGCTCTTGATCATTAGCCATTAGAGAAATGCAAATTAAAACTATGATG +A00126:224:H7HMWDSX3:3:1108:30544:19789 ! 0 Test_seq 167661 - - NR A00126:224:H7HMWDSX3:3:1108:30544:1978997Test_seq167316722S129M=167511346CCGTGGGTATTGATTGTGGATCGATCAGGCGCTGGTCTGAGATATCATAGCTGAATATTCTTTCCCAATCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGTTGTTTGATTTTTAGGAGCTCCCAGTTATCTGG:FFF:F:FFFF,F,FF:::FFF,F,FFF,FFF::FFFFFFFFFFFFFFFFFFFFFF,FF,:F:F,FFFFFFF:F,:FFFF:FFFFFF:FFFFFFF,F:FF:,FF,F:F:FFFFFFFFF,FF,FFF:FF:FFF:FF,FFFFF:F,F:FFF,FNM:i:5MD:Z:7A13G23G6A43G32MC:Z:151MAS:i:104XS:i:99XA:Z:Test_seq,-213590,129M22S,6;Yt:Z:NR A00126:224:H7HMWDSX3:3:1108:30544:19789145Test_seq16751160151M=167316-346TGATCCTATTTTTTCTTCCATGATCTTTATCGTTTTAGTCTTTATGTTCAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGFFFF::FFFFFFFFF:F,F,FFFFFFFF,FFFFFFFFFFFFF,FFFFFF,FFFFFFFFFFF::::FF:FFF:FFFFFF:FF:F:FFFFFFFFFFFFFF,FF::FFFF:FFFF:FFFFFFFFFFF::FFFFFFF::FFFFFFFFFFFF,FF:NM:i:1MD:Z:48T102MC:Z:22S129MAS:i:146XS:i:117XA:Z:Test_seq,+213377,83M2I23M2I37M4S,6;Yt:Z:NR 0 60 TGATCCTATTTTTTCTTCCATGATCTTTATCGTTTTAGTCTTTATGTTCAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTG +A00126:224:H7HMWDSX3:3:1108:8187:20400 Test_seq 77443 Test_seq 233514 + + RU A00126:224:H7HMWDSX3:3:1108:8187:2040065Test_seq7744360151M=233514156072TCCTCAGACAGTGCAATTTTCCTTGGCTTTTCAGCCCATAAAATTACTTGATAAACACTGTCTGCTTCTAAGCTTTCGGCCTTACACAGTTGCTTTGCTCCATTGTAAATAATTAAGAATTTAACAGGCCACTGATTAAAAACAAAATTGGFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF,,:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFF:::F:FFFFF:F,FFFF:F:FFFF:F:FFNM:i:1MD:Z:42G108MC:Z:27M124SAS:i:146XS:i:0Yt:Z:RU A00126:224:H7HMWDSX3:3:1108:8187:20400129Test_seq2335144827M124S=77443-156072CTCAAACCCATTGCCGTCGAGTTGATTACTCCATATTGGCAAGTGCAGCAAGGCTGGGGCCCCTTCAGGACATATAGAATATAGCCAACATGAGGCCCAGGGTCAAGATCAGGGGGCCATTTGGTAGCCTCCAAAAGCAAAGACTAACATCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFF:FFFFF:FFFFFFFFFFFF:FFFFFF::FFFFFF:FFFFFFFFFFFFF:FFFFFFFFFFF:FFFFFNM:i:0MD:Z:27MC:Z:151MAS:i:27XS:i:0SA:Z:Test_seq,77734,-,124M27S,60,0;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1108:8187:204002193Test_seq7773460124M27H=77443-415GATGTTAGTCTTTGCTTTTGGAGGCTACCAAATGGCCCCCTGATCTTGACCCTGGGCCTCATGTTGGCTATATTCTATATGTCCTGAAGGGGCCCCAGCCTTGCTGCACTTGCCAATATGGAGTFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFF::FFFFFF:FFFFFFFFFFFF:FFFFF:FFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFNM:i:0MD:Z:124MC:Z:151MAS:i:124XS:i:0SA:Z:Test_seq,233514,+,27M124S,48,0;Yt:Z:RU 60 48 TCCTCAGACAGTGCAATTTTCCTTGGCTTTTCAGCCCATAAAATTACTTGATAAACACTGTCTGCTTCTAAGCTTTCGGCCTTACACAGTTGCTTTGCTCCATTGTAAATAATTAAGAATTTAACAGGCCACTGATTAAAAACAAAATTGG CTCAAACCCATTGCCGTCGAGTTGATTACTCCATATTGGCAAGTGCAGCAAGGCTGGGGCCCCTTCAGGACATATAGAATATAGCCAACATGAGGCCCAGGGTCAAGATCAGGGGGCCATTTGGTAGCCTCCAAAAGCAAAGACTAACATC +A00126:224:H7HMWDSX3:3:1108:8495:20494 ! 0 Test_seq 162287 - + NR A00126:224:H7HMWDSX3:3:1108:8495:20494145Test_seq16242260126M25S=162287-261CTCAAAAACCCTATGGAAGAGTTCTTCTGTGCACACATGGGGTGGTCACGAGTCAGAATCGACTGGACTAGCAACTAACAACAGCAGGCAAGTTCGAAGGGTCCTCCTGGGTAGTTAGATGGGATCGATCTAATCTCTAAAATCTGTAGGAF:,F:F:FFFFFFFFFF,F:FFFFF:,FFFFFFF:F:FFFFFFFFFF,FF,:FF::,FFFF:FFFFFFFFFFFFFFFF:FFF:,FFF:F,FFFFFFFFFFFFF:FFFFFF,FFFFFFF:FFFFF:FFFF,,,FFFFFF:F::FFFFFFF:FNM:i:0MD:Z:126MC:Z:151MAS:i:126XS:i:0Yt:Z:NR A00126:224:H7HMWDSX3:3:1108:8495:2049497Test_seq16228760151M=162422261GGAGGAGGCAGGCAGAGGGAGGAGAAAAGCTTATTGGCTTATCATGGCATTAGTCATGTCAAGCTCACAGACACCCATTTCGAAAAGGAGAAAACAAGCTGGGGAGTATTAGGGTCACATTCTGAAAGGTAGAGCCTCAAAAACCCTATGGFFF:F:F,FFFFFFF:FFF:FFF:FFFFFF,FFFF:FF:FFF:FF::F:FFFF:FFFFFFFFFF:F,FFFFFFFFFFF,FFFFFFF:FFFFFFFFFFFFFFF::FFFFF,FF:FFFFFFFFFFFFFFFFFFF:,FFFFFFFFFF:FFFFFFNM:i:1MD:Z:66C84MC:Z:126M25SAS:i:146XS:i:0Yt:Z:NR 0 60 GGAGGAGGCAGGCAGAGGGAGGAGAAAAGCTTATTGGCTTATCATGGCATTAGTCATGTCAAGCTCACAGACACCCATTTCGAAAAGGAGAAAACAAGCTGGGGAGTATTAGGGTCACATTCTGAAAGGTAGAGCCTCAAAAACCCTATGG +A00126:224:H7HMWDSX3:3:1108:7744:20509 Test_seq 169333 Test_seq 170167 + - RU A00126:224:H7HMWDSX3:3:1108:7744:2050997Test_seq16933360151M=170144835GGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGATTCTTTTGTTGCTCACFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFFFFFF,FFFF,F:F,FFFFF:F:FF:NM:i:1MD:Z:134G16MC:Z:127S24MAS:i:146XS:i:109Yt:Z:RU A00126:224:H7HMWDSX3:3:1108:7744:205092193Test_seq16962660121M30H=169333-414CTCGTTGCTTTCTATGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTATTGTTCATTTTCCAAGTATTTGATTTCTTTTCCCTCGTTTTTCTGTTAFF:FFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFNM:i:4MD:Z:2T11A22G70A12MC:Z:151MAS:i:103XS:i:0SA:Z:Test_seq,170144,-,127S24M,30,0;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1108:7744:20509145Test_seq17014430127S24M=169333-835CTCGTTGCTTTCTATGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTATTGTTCATTTTCCAAGTATTTGATTTCTTTTCCCTCGTTTTTCTGTTATTGATCGATCAATTGTCCCTTTTATCATTAFF:FFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFNM:i:0MD:Z:24MC:Z:151MAS:i:24XS:i:0SA:Z:Test_seq,169626,-,121M30S,60,4;Yt:Z:RU 60 30 GGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGATTCTTTTGTTGCTCAC CTCGTTGCTTTCTATGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTATTGTTCATTTTCCAAGTATTTGATTTCTTTTCCCTCGTTTTTCTGTTATTGATCGATCAATTGTCCCTTTTATCATTA +A00126:224:H7HMWDSX3:3:1108:16233:21339 Test_seq 197163 Test_seq 197474 + - UU A00126:224:H7HMWDSX3:3:1108:16233:21339161Test_seq19716360151M=197343312TCACATTTTTCTCCCAGCATACGCATGCTGATGTGGGGTTTTGTCATGTTTCTTAAGGGAACTGTGATGAAAATTCTCCTCTGACAGACATGTCTCAACTTCCAGATGGTAAGAAATTTTGTGAGAGTGATGAAATTAAGATACTTGATGCFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFFF:FFFFFF:F:FFFFFFFFFFFFFFF,FFFFFFFFFF:,F:FFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:132M19SAS:i:151XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1108:16233:2133981Test_seq19734360132M19S=197163-312ATTCTAGGGGGAATTGCTTCCATGACAGTGTATTGAACCTGTGCTCCAAGGGGTGCCTTTAACAGTGGACTTGGTATCCTCTGGAGTTGTACAACGTAATTGCTCTGAGCCCAGAGCCCAGGAAATAAGATCGATCTTGATTTTCAGTACGFFF:,FF:FFFFFFF:FFFF:FFFFF:FFF:FFFFF:::FFFFFFF:F,FF:FFF::FF:FF,FFFFFF:FFFFFFFFFFFFFFFFFF:FFF::FF:FFFFFFFFFFFFFF::FFF:FF:FFF,FFFF:FFFFFFFFFF,FF:FFFFFFFFNM:i:0MD:Z:132MC:Z:151MAS:i:132XS:i:0Yt:Z:UU 60 60 TCACATTTTTCTCCCAGCATACGCATGCTGATGTGGGGTTTTGTCATGTTTCTTAAGGGAACTGTGATGAAAATTCTCCTCTGACAGACATGTCTCAACTTCCAGATGGTAAGAAATTTTGTGAGAGTGATGAAATTAAGATACTTGATGC ATTCTAGGGGGAATTGCTTCCATGACAGTGTATTGAACCTGTGCTCCAAGGGGTGCCTTTAACAGTGGACTTGGTATCCTCTGGAGTTGTACAACGTAATTGCTCTGAGCCCAGAGCCCAGGAAATAAGATCGATCTTGATTTTCAGTACG +A00126:224:H7HMWDSX3:3:1108:16342:23437 ! 0 Test_seq 171131 - + NR A00126:224:H7HMWDSX3:3:1108:16342:23437145Test_seq17129460128M23S=171131-291TAAATGGGGTTCGATGAGCATCTTGGATAGATATCCTTTCGTCTTTCATGATGTCAGGGAAGTTTTGTGTCAGGAGTTCTTCAACTATTTTCTCTGTGTTTTCTGTCCCCCCTCCCTGTTCTGGGACTTAATGGATGTGTGCAGCTTTTCT:FFF:,FFFFFFFFFF:FFF,FFFFFF:FFFFFFFFFFFFF,FFFFFFFFFFFFFFFFF:FF:FF:FFF:FFFF:FFFFF:FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFFFF:FFFFF:FFFFFNM:i:2MD:Z:106G15T5MC:Z:151MAS:i:118XS:i:43Yt:Z:NR A00126:224:H7HMWDSX3:3:1108:16342:2343797Test_seq17113160151M=171294291GCCTGCATGGTTTCTGCTGAGTAGTCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTATCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFF:FFFFFFFFF::FFFF,FF,:FFF,FFFFFFFFFFFFF:FFFFFFFF,FF:F:FFF,FFFFF:FFFFF:FF:NM:i:1MD:Z:71C79MC:Z:128M23SAS:i:146XS:i:75Yt:Z:NR 0 60 GCCTGCATGGTTTCTGCTGAGTAGTCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTATCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTT +A00126:224:H7HMWDSX3:3:1108:16740:23625 Test_seq 170381 Test_seq 170830 + - UU A00126:224:H7HMWDSX3:3:1108:16740:23625161Test_seq17038160151M=170680450CTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTTTTCATGTGTGTTGTTGGCCTTTTCATTTTTCCACATACTTTTTTGTGCTGCGACGTTTTTCF:FFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF::F:FFFFF:F,FF:FFFFFFF:FF:NM:i:1MD:Z:140A10MC:Z:151MAS:i:146XS:i:47Yt:Z:UU A00126:224:H7HMWDSX3:3:1108:16740:2362581Test_seq17068060151M=170381-450GCGTTCTATATCACCTTGTATCACTCTCCATCTGGTAGTTCAATGCCTCCTATATTTAGTCCCTCTTTTTGATTATTGTGATCGTTTATCTATTGATTTCCATGATTTCCTGTTATGTGTATTATTTTGTTTATTTATTTATTTTTTAGAAF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFNM:i:4MD:Z:0T4G29C15G99MC:Z:151MAS:i:135XS:i:0Yt:Z:UU 60 60 CTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTTTTCATGTGTGTTGTTGGCCTTTTCATTTTTCCACATACTTTTTTGTGCTGCGACGTTTTTC GCGTTCTATATCACCTTGTATCACTCTCCATCTGGTAGTTCAATGCCTCCTATATTTAGTCCCTCTTTTTGATTATTGTGATCGTTTATCTATTGATTTCCATGATTTCCTGTTATGTGTATTATTTTGTTTATTTATTTATTTTTTAGAA +A00126:224:H7HMWDSX3:3:1108:25789:25488 ! 0 Test_seq 239267 - + NR A00126:224:H7HMWDSX3:3:1108:25789:2548881Test_seq23939060122M29S=239267-245TTTTCAATGGCTGCTTTTTCAGAAGTAGATAGACAAGTCTTTCTTCGACGTGCTTCTGGGTCAACTTGTTACCAAACCGAACTCGGGCCCCTCCCTGCCCGGTGCCTGCAGAGTTCCAATTAGATCTTCAGGTTTTGAATCACAGAACATTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFNM:i:0MD:Z:122MC:Z:151MAS:i:122XS:i:0Yt:Z:NR A00126:224:H7HMWDSX3:3:1108:25789:25488161Test_seq23926760151M=239390245CATTTTTAAATGATGTCAAATTTATTTATGGATATTGAATACAATAAAAATCAGTTGCCATCCAGTAGATTTCTACTCATGGCGATCCACATACGTCACAGTAGAACTGTGTTCCATGGGGTTTTTTCAATGGCTGCTTTTTCAGAAGTAGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:122M29SAS:i:151XS:i:0Yt:Z:NR 0 60 CATTTTTAAATGATGTCAAATTTATTTATGGATATTGAATACAATAAAAATCAGTTGCCATCCAGTAGATTTCTACTCATGGCGATCCACATACGTCACAGTAGAACTGTGTTCCATGGGGTTTTTTCAATGGCTGCTTTTTCAGAAGTAG +A00126:224:H7HMWDSX3:3:1108:25491:25661 Test_seq 240923 Test_seq 241300 + - UU A00126:224:H7HMWDSX3:3:1108:25491:2566197Test_seq24092360151M=241150378GATGAGCTCAAGCATAACGATTGTAAGGATGGCACAGGACTGGGCAGTGTTTCGTTCTGTTGTGCATAGGGTCGCTATGAGTTGGTACCGACTGGACAGCACCTAACAACAACAACAATGGAGAAGTTCAAACAAAGACAAAATGGGCCTCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,,FF::FF::,F,F,F:,FF:FFFFF:F,FFFFF:FF:FFF:F,,F,F,F::F,FFF:FF,FF::F:F,,FFFF,:NM:i:2MD:Z:85A49T15MC:Z:151MAS:i:141XS:i:59Yt:Z:UU A00126:224:H7HMWDSX3:3:1108:25491:25661145Test_seq24115060151M=240923-378CTAAAATTTTATGACTCTGGACTATGTTTGACCTGCTTGAAGAAAATGTTCTAATCAATAATCCTTGTCACTTTCCAAAATTTAAGTCCTTGTTCTGGAAGAGGCTTAAACTGAGAAGATAAAGGGGGAAAAAATCAGTACCAAACCAGGTFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU 60 60 GATGAGCTCAAGCATAACGATTGTAAGGATGGCACAGGACTGGGCAGTGTTTCGTTCTGTTGTGCATAGGGTCGCTATGAGTTGGTACCGACTGGACAGCACCTAACAACAACAACAATGGAGAAGTTCAAACAAAGACAAAATGGGCCTC CTAAAATTTTATGACTCTGGACTATGTTTGACCTGCTTGAAGAAAATGTTCTAATCAATAATCCTTGTCACTTTCCAAAATTTAAGTCCTTGTTCTGGAAGAGGCTTAAACTGAGAAGATAAAGGGGGAAAAAATCAGTACCAAACCAGGT +A00126:224:H7HMWDSX3:3:1108:26033:26694 Test_seq 170197 Test_seq 170521 + - UU A00126:224:H7HMWDSX3:3:1108:26033:26694161Test_seq170197604S147M=170371325GGTGGATTTAAGTCTAAAGTCTATTTTTTCAGAAATTAATATTGCTACTCTTCTTCTTTTTTGCTTATTATTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGFFFF,FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFF:F:FF:FFFF:FFFFFFFFF::FFFFFFFFFF::FFF,FFFFFFFFFFF:FFFFFFFFFF:FFFFFFFF:FFFF:FF,FFF:FFFFFF:,FF:F:F,FFFFF:FFFF,FNM:i:2MD:Z:23G22C100MC:Z:151MAS:i:137XS:i:69Yt:Z:UU A00126:224:H7HMWDSX3:3:1108:26033:2669481Test_seq17037160151M=170197-325CAGTCCGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTTTTCATGTGTGTTGTTGGCCATTTCATTTTTCCACATACTTTTTTGTGCTGAFFFFFFFFF,FFFF,FFF:FFFFFFFF,FFFFFFFFFFF:FFF:FF::FFFF,F:FFFFFFFFFFFFF,F,F,FFFFFFFFFFFFF,FFFFF:FFF:,FFFFFFFFF:F:FFFFFFF:,F:FF:FFFFFFF,F,FFFF:FF:FFFFFFFFFNM:i:1MD:Z:119T31MC:Z:4S147MAS:i:146XS:i:48Yt:Z:UU 60 60 GGTGGATTTAAGTCTAAAGTCTATTTTTTCAGAAATTAATATTGCTACTCTTCTTCTTTTTTGCTTATTATTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAG CAGTCCGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTTTTCATGTGTGTTGTTGGCCATTTCATTTTTCCACATACTTTTTTGTGCTGA +A00126:224:H7HMWDSX3:3:1108:3251:26850 Test_seq 166211 Test_seq 214877 - - UU A00126:224:H7HMWDSX3:3:1108:3251:26850177Test_seq1661582554M=21482448667TCAGTATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCTTFFFF::F,FF,FFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:4G49MC:Z:54MAS:i:49XS:i:41XA:Z:Test_seq,+214829,5S49M,2;Yt:Z:UU A00126:224:H7HMWDSX3:3:1108:3251:26850113Test_seq2148242654M=166158-48667AAGAGCCCAAGAGACAGAAAGGGCCACATGAACCAGAGACCTACATCATCCTGA:F,:,FFFFFFF,FFFFF:F,FFF:FFFF:FFF:FFF:FFFF,,FFFFFFFFFFNM:i:1MD:Z:1T52MC:Z:54MAS:i:52XS:i:44XA:Z:Test_seq,+166158,54M,2;Yt:Z:UU 25 26 TCAGTATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCTT AAGAGCCCAAGAGACAGAAAGGGCCACATGAACCAGAGACCTACATCATCCTGA +A00126:224:H7HMWDSX3:3:1108:3378:27226 Test_seq 72787 Test_seq 73208 + - UU A00126:224:H7HMWDSX3:3:1108:3378:2722697Test_seq7278760151M=73058422AAGGAGAAAAAAGGAAATATTTGTACCCTGTATGCAAATATCTTGGAATGGAGCAGTAGTCTGCAGTAATGGGGGAACTGAAAAATTCCTAGAAGCTGTCAGCCTGAAATGAATATTTTATAAACATATATGATTTGGAGGCTTATATAATFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFF::FFFFFFF:FFFFFFF,FFFFFFFFFFFFF:F:FFFFFFFFFF,FFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFF:FFFFFF:FFFFFF,FFF,FFFF:FFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1108:3378:27226145Test_seq7305860151M=72787-422AGGTTCTCACATGGATTCTCATGAAAATTATATGAGCACGTTGTATTAATTTGTTTAAAAAAAAAATTGGTACTTTTTTATTGGCTTTGCTTCTTTTGGTTCTTACTGCCTATATCAGAAGACGTTGTATTCATTTAAAGGAAGAGCATTCF,FF,FF:FFFFF,:FF,FFFF,FF::FFFFFFFFF:FF:FFFFFFFF:FFFF,FFFF:FFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFF:FFFFF,FFFFF:FFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFFF:FFF,FFFFFFFFNM:i:2MD:Z:4G48G97MC:Z:151MAS:i:141XS:i:0Yt:Z:UU 60 60 AAGGAGAAAAAAGGAAATATTTGTACCCTGTATGCAAATATCTTGGAATGGAGCAGTAGTCTGCAGTAATGGGGGAACTGAAAAATTCCTAGAAGCTGTCAGCCTGAAATGAATATTTTATAAACATATATGATTTGGAGGCTTATATAAT AGGTTCTCACATGGATTCTCATGAAAATTATATGAGCACGTTGTATTAATTTGTTTAAAAAAAAAATTGGTACTTTTTTATTGGCTTTGCTTCTTTTGGTTCTTACTGCCTATATCAGAAGACGTTGTATTCATTTAAAGGAAGAGCATTC +A00126:224:H7HMWDSX3:3:1108:13639:27524 Test_seq 330730 Test_seq 331086 + - UU A00126:224:H7HMWDSX3:3:1108:13639:27524161Test_seq33073060151M=330936357AAAAGGTCAATCAAGTAGCCAGGAGTCCCAGGTGGCAGGGCTGGTTAACTGGAATATTAAGTCACTGAGTTTCCAATACAACTCTTGGCCCAACAACCAAAAATACCTATTTCATTTTTGCTTCTGGGTGAATTTCAGGAGTTGGGGGTTAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFF:FNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1108:13639:2752481Test_seq33093660151M=330730-357AACTCATGGCAACTCTAGGAAACAGAATGGGTATGGCTTTATTGGAGTCCTTAAATTCTATGGCTGGCCTGCATCGTTCTCATTGATCCTAGCCCTATGTAATCAGGTTCCTCTTCCTACCCAGCTGCTGGCTCTTCCTGTGTTTGTTAGGFFFFF,FFFFFFFFFFFFFF,FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU 60 60 AAAAGGTCAATCAAGTAGCCAGGAGTCCCAGGTGGCAGGGCTGGTTAACTGGAATATTAAGTCACTGAGTTTCCAATACAACTCTTGGCCCAACAACCAAAAATACCTATTTCATTTTTGCTTCTGGGTGAATTTCAGGAGTTGGGGGTTA AACTCATGGCAACTCTAGGAAACAGAATGGGTATGGCTTTATTGGAGTCCTTAAATTCTATGGCTGGCCTGCATCGTTCTCATTGATCCTAGCCCTATGTAATCAGGTTCCTCTTCCTACCCAGCTGCTGGCTCTTCCTGTGTTTGTTAGG +A00126:224:H7HMWDSX3:3:1108:26223:27837 Test_seq 170197 Test_seq 170521 + - UU A00126:224:H7HMWDSX3:3:1108:26223:27837161Test_seq170197604S147M=170371325GGTGGATTTAAGTCTAAAGTCTATTTTTTCAGAAATTAATATTGCTACTCTTCTTCTTTTTTGCTTATTATTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFF:FFFFFFFFFF:FFFFFFFFFFF::F:FFFF,FFFFFFFFF,FFFF:FFFFFFF:FFFFFFF:FFFFNM:i:2MD:Z:23G22C100MC:Z:151MAS:i:137XS:i:69Yt:Z:UU A00126:224:H7HMWDSX3:3:1108:26223:2783781Test_seq17037160151M=170197-325CAGTCCGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTTTTCATGTGTGTTGTTGGCCATTTCATTTTTCCACATACTTTTTTGTGCTGAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:119T31MC:Z:4S147MAS:i:146XS:i:48Yt:Z:UU 60 60 GGTGGATTTAAGTCTAAAGTCTATTTTTTCAGAAATTAATATTGCTACTCTTCTTCTTTTTTGCTTATTATTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAG CAGTCCGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTTTTCATGTGTGTTGTTGGCCATTTCATTTTTCCACATACTTTTTTGTGCTGA +A00126:224:H7HMWDSX3:3:1108:14561:28056 Test_seq 168438 Test_seq 168700 + - UU A00126:224:H7HMWDSX3:3:1108:14561:2805697Test_seq16843860151M=168550263GACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFNM:i:1MD:Z:57T93MC:Z:151MAS:i:146XS:i:86Yt:Z:UU A00126:224:H7HMWDSX3:3:1108:14561:28056145Test_seq16855060151M=168438-263GGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATGAATGGGTGTTAGACTTTGTCAAATGCCTTTTCTGCATCAGTTGATAAGATCATGTGGTTTTTGTCTTTTGTTTTATFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFF::FFFFNM:i:6MD:Z:74A10G0A26T0A22A13MC:Z:151MAS:i:121XS:i:81Yt:Z:UU 60 60 GACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTT GGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATGAATGGGTGTTAGACTTTGTCAAATGCCTTTTCTGCATCAGTTGATAAGATCATGTGGTTTTTGTCTTTTGTTTTAT +A00126:224:H7HMWDSX3:3:1108:9968:28338 Test_seq 170323 Test_seq 170373 + - UU A00126:224:H7HMWDSX3:3:1108:9968:28338161Test_seq1703236051M=17032351AGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:FFFFFFF,FFFFFFFFFFFFNM:i:0MD:Z:51MC:Z:51MAS:i:51XS:i:29Yt:Z:UU A00126:224:H7HMWDSX3:3:1108:9968:2833881Test_seq1703231251M=170323-51AGGTGTGTCTCTTGTAGGCAGCATATAGACAGATCGTGTTTCTTTATCCAGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:2MD:Z:29T0G20MC:Z:51MAS:i:41XS:i:36XA:Z:Test_seq,+38373,15S36M,0;Yt:Z:UU 60 12 AGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAG AGGTGTGTCTCTTGTAGGCAGCATATAGACAGATCGTGTTTCTTTATCCAG +A00126:224:H7HMWDSX3:3:1108:5285:30373 Test_seq 167167 Test_seq 167535 + - UU A00126:224:H7HMWDSX3:3:1108:5285:3037397Test_seq16716752151M=167401369GCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAATCATGTAGATTTTAGAGAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFF:FFF,:F:FFFF:F:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:76T74MC:Z:135M16SAS:i:146XS:i:121XA:Z:Test_seq,-213717,151M,6;Yt:Z:UU A00126:224:H7HMWDSX3:3:1108:5285:30373145Test_seq16740119135M16S=167167-369GATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATACCTTGTATTAGGGCTCCTAGGGTTGTCCCAATTTTTTCTTCCATGATCGATCTAGAAAAAAAATFFFFFF:FFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFF,FFFFFFFFFFFNM:i:5MD:Z:86G2C22A0T2T18MC:Z:151MAS:i:110XS:i:100XA:Z:Test_seq,+213499,16S135M,7;Yt:Z:UU 52 19 GCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAATCATGTAGATTTTAGAGA GATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATACCTTGTATTAGGGCTCCTAGGGTTGTCCCAATTTTTTCTTCCATGATCGATCTAGAAAAAAAAT +A00126:224:H7HMWDSX3:3:1108:23592:31234 ! 0 Test_seq 405523 - + NR A00126:224:H7HMWDSX3:3:1108:23592:3123481Test_seq40567660127M24S=405523-280GCATGAACATGGATCTCTTCTAGTCAGTTGCAGATCCTTGTAATTTCCACTGTTACCTATCTTTACCCCCCACTTACTTATAAAGTTTCTAGTTTTGCAGATTGTTCAGTTGTTGTTGTTGTTTTTATAAAGCAAAAATGTCATCAATGTCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:127MC:Z:151MAS:i:127XS:i:0Yt:Z:NR A00126:224:H7HMWDSX3:3:1108:23592:31234161Test_seq40552360151M=405676280CCTTTTCCGTAGTTTGTTATTGCGGTAGTTCCCTGTTGATGTACTGCTGTAACTGGTTTTGAATTATCTTCAGCAAAATTTTACTTGCATGTGATATTGCTAGTATCGTGCGGTACTCTTCACATTCTGTCGGATCTCCTTTCTTTGGAACFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:101G49MC:Z:127M24SAS:i:146XS:i:0Yt:Z:NR 0 60 CCTTTTCCGTAGTTTGTTATTGCGGTAGTTCCCTGTTGATGTACTGCTGTAACTGGTTTTGAATTATCTTCAGCAAAATTTTACTTGCATGTGATATTGCTAGTATCGTGCGGTACTCTTCACATTCTGTCGGATCTCCTTTCTTTGGAAC +A00126:224:H7HMWDSX3:3:1108:15745:32706 Test_seq 213581 Test_seq 213924 + - UU A00126:224:H7HMWDSX3:3:1108:15745:3270697Test_seq21358132151M=213774344GAAGAGAAACCAGATAACTGGGAGCTCCTAAAAATCAAACACCTATGCTCATCTGAAGACTTCACCAAAAGAGTAAAAAGACGACCTACAGATTGGGAAAGAATTTTCAGCTATGACATCTCCGACCAGCGCCTCATCTCTAAAATCTATAFF:FFFFFFFFFF:F::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFF:FFFFFFFFF:FFF:FFFFFFFFFFFF:F,F:FF:FFFFNM:i:5MD:Z:54A27C5T3C41G16MC:Z:151MAS:i:126XS:i:109XA:Z:Test_seq,-167303,151M,9;Test_seq,-400724,136M15S,7;Yt:Z:UU A00126:224:H7HMWDSX3:3:1108:15745:32706145Test_seq21377460151M=213581-344TCAAGAAGTGGGCAAAGGATATGAACACGCACTTCACTAAAGAAGATATTCAGGCAGCTAACAGATACATGAGAAAATGCTCTCGATCATTAGCCATTAGAGAAATGCAAATTAAAACTACGATGAGATTCCATCTCACTCCAACAAGGCTFFFFFFFFFFFF:FFFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:117G33MC:Z:151MAS:i:146XS:i:115Yt:Z:UU 32 60 GAAGAGAAACCAGATAACTGGGAGCTCCTAAAAATCAAACACCTATGCTCATCTGAAGACTTCACCAAAAGAGTAAAAAGACGACCTACAGATTGGGAAAGAATTTTCAGCTATGACATCTCCGACCAGCGCCTCATCTCTAAAATCTATA TCAAGAAGTGGGCAAAGGATATGAACACGCACTTCACTAAAGAAGATATTCAGGCAGCTAACAGATACATGAGAAAATGCTCTCGATCATTAGCCATTAGAGAAATGCAAATTAAAACTACGATGAGATTCCATCTCACTCCAACAAGGCT +A00126:224:H7HMWDSX3:3:1108:30246:32957 Test_seq 170315 Test_seq 170762 + - UU A00126:224:H7HMWDSX3:3:1108:30246:32957161Test_seq17031560151M=170621448TAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCTGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATTTTGATFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFNM:i:1MD:Z:61C89MC:Z:9S142MAS:i:146XS:i:48Yt:Z:UU A00126:224:H7HMWDSX3:3:1108:30246:3295781Test_seq170621609S142M=170315-448GTTGTTATACCTTTTTGTGGTTACCTTATTATTTACCCCTATTTTTCTAAGTAAAAATCTAACTTGTATTGTTCTATATCGCCTTGTATCACTCTCCATATGGCAGTTCAATGCCTCCTGTATTTAGTCCCTCTTTTTGATTATTTTGATC:FFFFF::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:6MD:Z:48C11C3G6A18C45G5MC:Z:151MAS:i:112XS:i:37Yt:Z:UU 60 60 TAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCTGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATTTTGAT GTTGTTATACCTTTTTGTGGTTACCTTATTATTTACCCCTATTTTTCTAAGTAAAAATCTAACTTGTATTGTTCTATATCGCCTTGTATCACTCTCCATATGGCAGTTCAATGCCTCCTGTATTTAGTCCCTCTTTTTGATTATTTTGATC +A00126:224:H7HMWDSX3:3:1108:3079:34131 Test_seq 18385 Test_seq 214177 + + UU A00126:224:H7HMWDSX3:3:1108:3079:3413165Test_seq183851351M=214177195793ATCCATTCATCCATTGATGGGCACCTTGGTTGCTTCCATCTTTTTGCTATTFFFFFFFFFFFFFFFFFFFF,FF,FFFFFFFFFFFFFFFFFFFFF:FFFFFNM:i:0MD:Z:51MC:Z:51MAS:i:51XS:i:46XA:Z:Test_seq,+48772,51M,1;Test_seq,-41848,51M,1;Yt:Z:UU A00126:224:H7HMWDSX3:3:1108:3079:34131129Test_seq214177351M=18385-195793AATAGCAAAAAGCTGGAAGCAACCAAGGTGTCTATCAACGGATGAATGGATFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFF:F:FFFFFFFFF::FNM:i:3MD:Z:32C5T10T1MC:Z:51MAS:i:39XS:i:36XA:Z:Test_seq,-48772,51M,3;Test_seq,+41848,51M,3;Test_seq,-400228,51M,4;Yt:Z:UU 13 3 ATCCATTCATCCATTGATGGGCACCTTGGTTGCTTCCATCTTTTTGCTATT AATAGCAAAAAGCTGGAAGCAACCAAGGTGTCTATCAACGGATGAATGGAT +A00126:224:H7HMWDSX3:3:1108:19533:34569 ! 0 Test_seq 168590 - - MU A00126:224:H7HMWDSX3:3:1108:19533:34569177Test_seq2127380151M=168440-44299ACTAATAGAAGAGTTTGGCAGAGTCTCAGGTTATAAAATAAACATACAAAAATCACTTGGATTCCTCTACATCAACAAAAAGAACACCGAAGAGGAAATCACCAAATCAATACCATTCACAGTAGCCCCCAAGAAGATAAAATACTTAGGAFFFFFFF,FFFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:4MD:Z:36G49T1A10A51MC:Z:151MAS:i:131XS:i:131XA:Z:Test_seq,+168150,151M,4;Yt:Z:MU A00126:224:H7HMWDSX3:3:1108:19533:34569113Test_seq16844060151M=21273844299CTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:55T95MC:Z:151MAS:i:146XS:i:88Yt:Z:MU 0 60 ACTAATAGAAGAGTTTGGCAGAGTCTCAGGTTATAAAATAAACATACAAAAATCACTTGGATTCCTCTACATCAACAAAAAGAACACCGAAGAGGAAATCACCAAATCAATACCATTCACAGTAGCCCCCAAGAAGATAAAATACTTAGGA CTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCC +A00126:224:H7HMWDSX3:3:1108:20112:34757 ! 0 Test_seq 168604 - - NR A00126:224:H7HMWDSX3:3:1108:20112:3475797Test_seq168195825S126M=168454410TACATCCTTGGTAAGATTTATTCCTTTTGGTTATTTCCTCTTCGGTGTTCTTTTTGTTGATGTAGAGGAATCCAAGTGATTTTTGTATGTTTATTTTATAACATGAGACTCTGCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGAFFFFFF::F:FFFFF:FFFFFFFFFFFFF:FFF::FF,F:FFFF,::FFFFFF:FFFFFFFFFFFF,FFFFF:FFFF:FF,,FFFFFFF:FFFFFFFFFFFF,FFF:FFFFFFF,FFF,F:FFFFFF,FF:F:FFFFFF:FFF:FF:FF,FNM:i:3MD:Z:76T0C9T38MC:Z:151MAS:i:111XS:i:106XA:Z:Test_seq,-212718,126M25S,4;Yt:Z:NR A00126:224:H7HMWDSX3:3:1108:20112:34757145Test_seq16845460151M=168195-410TTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTF:FF,F,FFFFFFF:FFFFFFFFFFFFFFFFFFFFF::FFF,F,FFFFFF:FFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFF::FFFFFF:FF:FFFF::FF:FF,FFFFFF:FFF:FF,FFFFFF:FF:FF,FF:NM:i:1MD:Z:41T109MC:Z:25S126MAS:i:146XS:i:90Yt:Z:NR 0 60 TTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTT +A00126:224:H7HMWDSX3:3:1108:20374:35243 ! 0 Test_seq 168604 - - NR A00126:224:H7HMWDSX3:3:1108:20374:3524397Test_seq168195825S126M=168454410TACATCCTTGGTAAGATTTATTCCTTTTGGTTATTTCCTCTTCGGTGTTCTTTTTGTTGATGTAGAGGAATCCAAGTGATTTTTGTATGTTTATTTTATAACCTGAGACTCTGCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGAFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:FNM:i:2MD:Z:76T10T38MC:Z:151MAS:i:116XS:i:111XA:Z:Test_seq,-212718,126M25S,3;Yt:Z:NR A00126:224:H7HMWDSX3:3:1108:20374:35243145Test_seq16845460151M=168195-410TTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFFF:F,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:41T109MC:Z:25S126MAS:i:146XS:i:90Yt:Z:NR 0 60 TTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTT +A00126:224:H7HMWDSX3:3:1108:22788:35509 ! 0 Test_seq 168739 - + MR A00126:224:H7HMWDSX3:3:1108:22788:3550965Test_seq251240023M128S=168739-82502GTCCTATAGGGTTGCTATGAGTTAACACATTCAGAAGTAGAACAACTAAATAGACCCATAACAAAAAAAGAGATTGAAACGGTAATCAAAAAACTTCCAACAACAAAAAGTCCTGGCCCAGACGGCTTCACTGCAGAGTTCTACCAAACCTFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFF:,F:FFFFFFF:FFFF:F:FFFFFFNM:i:0MD:Z:23MC:Z:151MAS:i:23XS:i:23SA:Z:Test_seq,169010,-,129M22S,60,1;XA:Z:Test_seq,-310625,128S23M,0;Test_seq,+365190,22M129S,0;Yt:Z:MRNEXT_SAMA00126:224:H7HMWDSX3:3:1108:22788:355092129Test_seq16901060129M22H=168739-400AGGTTTGGTAGAACTCTGCAGTGAAGCCGTCTGGGCCAGGACTTTTTGTTGTTGGAAGTTTTTTGATTACCGTTTCAATCTCTTTTTTTGTTATGGGTCTATTTAGTTGTTCTACTTCTGAATGTGTTAFFFFFF:F:FFFF:FFFFFFF:F,:FFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF:NM:i:1MD:Z:47T81MC:Z:151MAS:i:124XS:i:53SA:Z:Test_seq,251240,+,23M128S,0,0;Yt:Z:MR A00126:224:H7HMWDSX3:3:1108:22788:35509129Test_seq16873960151M=25124082502AACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGTATTATTTTTTTGATGTGTTGTTGGATTCTATTGGCTAGAATTTTGTTGAGGATTTTTGCATCTATGTTCATGAGGGATATAGGTCTAAAATTTTCTTTTTTTGTAAFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:F:FFFF:::,FFFFFFFFFFFFFFFFFFF:FF,FFFFFFF,:FFFFFFFFFFF,FFFFFFFFFFFFF:F:FFFFF:FF,FFFFFFF:F:,FFFFFFFFFFFF,F:F,FFFFFFFFFF,NM:i:1MD:Z:44A106MC:Z:23M128SAS:i:146XS:i:81Yt:Z:MR 0 60 GTCCTATAGGGTTGCTATGAGTTAACACATTCAGAAGTAGAACAACTAAATAGACCCATAACAAAAAAAGAGATTGAAACGGTAATCAAAAAACTTCCAACAACAAAAAGTCCTGGCCCAGACGGCTTCACTGCAGAGTTCTACCAAACCT AACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGTATTATTTTTTTGATGTGTTGTTGGATTCTATTGGCTAGAATTTTGTTGAGGATTTTTGCATCTATGTTCATGAGGGATATAGGTCTAAAATTTTCTTTTTTTGTAA +A00126:224:H7HMWDSX3:3:1108:32416:35650 Test_seq 379713 Test_seq 381176 + - UR A00126:224:H7HMWDSX3:3:1108:32416:35650161Test_seq3797134226M125S=3810261464GTGCTCCTTCCGCCTGCCTTCTGATCAATCTGGCTCAGGTGCATGCTCTTAGCCGTTATGCTACATTGAAGGCAGGAGGATCAGGTAGATAAAGACAGAGGAGGAAAGTTTGGGGAGCGTATTTGAGAAGCAGTATTACAAATCGCTGAGTFFFFFFFFFFFFFFFFFF,FFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFF,FF:FFFFFFFFFFFFFFFFFFFFFFF:F::FFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFNM:i:0MD:Z:26MC:Z:151MAS:i:26XS:i:0SA:Z:Test_seq,380936,+,26S125M,60,0;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1108:32416:356502209Test_seq3809366026H125M=381026241AATCTGGCTCAGGTGCATGCTCTTAGCCGTTATGCTACATTGAAGGCAGGAGGATCAGGTAGATAAAGACAGAGGAGGAAAGTTTGGGGAGCGTATTTGAGAAGCAGTATTACAAATCGCTGAGTFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFF,FF:FFFFFFFFFFFFFFFFFFFFFFF:F::FFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFNM:i:0MD:Z:125MC:Z:151MAS:i:125XS:i:0SA:Z:Test_seq,379713,+,26M125S,42,0;Yt:Z:UR A00126:224:H7HMWDSX3:3:1108:32416:3565081Test_seq38102660151M=379713-1464GCGTATTTGAGAAGCAGTATTACAAATCGCTGAGTATTAAATGTCCTTCTGGTTGTTTGTATTTGTCTTCCTTTCCCTTTGTCCATGGTTATCCATGCCTACGGAGGGAGAGCAGGAATGTATAATACAAAATCCTGCGGTAATCTGTGCGF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FF:,FFFFFFFFFFF:FFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFF:FFF,FFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:116C34MC:Z:26M125SAS:i:146XS:i:0Yt:Z:UR 42 60 GTGCTCCTTCCGCCTGCCTTCTGATCAATCTGGCTCAGGTGCATGCTCTTAGCCGTTATGCTACATTGAAGGCAGGAGGATCAGGTAGATAAAGACAGAGGAGGAAAGTTTGGGGAGCGTATTTGAGAAGCAGTATTACAAATCGCTGAGT GCGTATTTGAGAAGCAGTATTACAAATCGCTGAGTATTAAATGTCCTTCTGGTTGTTTGTATTTGTCTTCCTTTCCCTTTGTCCATGGTTATCCATGCCTACGGAGGGAGAGCAGGAATGTATAATACAAAATCCTGCGGTAATCTGTGCG +A00126:224:H7HMWDSX3:3:1108:7591:35837 Test_seq 170207 Test_seq 170534 + - UU A00126:224:H7HMWDSX3:3:1108:7591:35837161Test_seq17020760151M=170384328TAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCCTCTTCTTTTTTGCTTATTGTTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:55A95MC:Z:151MAS:i:146XS:i:75Yt:Z:UU A00126:224:H7HMWDSX3:3:1108:7591:3583781Test_seq17038460151M=170207-328TCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGGGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTTTTTATGTGTGTTGTTGACAATTTCATTTTTCCACATACTTTTTTGTGCCGAGGCGTCTTTCTTAFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:8MD:Z:40C48C13G1C0T28T3A3T7MC:Z:151MAS:i:111XS:i:51Yt:Z:UU 60 60 TAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCCTCTTCTTTTTTGCTTATTGTTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATC TCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGGGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTTTTTATGTGTGTTGTTGACAATTTCATTTTTCCACATACTTTTTTGTGCCGAGGCGTCTTTCTTA +A00126:224:H7HMWDSX3:3:1108:10122:36902 Test_seq 167329 Test_seq 167702 + - UU A00126:224:H7HMWDSX3:3:1108:10122:36902161Test_seq1673292120S131M=167552374GCGATGTACTTCAGGGTAGATCTGAGATGTCATAGCTGAATATTCTTTCCCAATCTGTAGGTGGGCATTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCTGCTTTCTCTTCATCATTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:FF,:FFFF:FFFFFFFFFFFFF:FFFFFFF:FFFF:F:F,:F:FFFF:FFFFFNM:i:5MD:Z:32G6A4T1T68G15MC:Z:151MAS:i:106XS:i:95XA:Z:Test_seq,-213575,128M23S,7;Yt:Z:UU A00126:224:H7HMWDSX3:3:1108:10122:3690281Test_seq16755234151M=167329-374TTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATCATTTCCCCAATTGACTGACACTGGTCCFFFFFFFFF:F:FFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:20S131MAS:i:151XS:i:135XA:Z:Test_seq,+213336,124M2I25M,4;Yt:Z:UU 21 34 GCGATGTACTTCAGGGTAGATCTGAGATGTCATAGCTGAATATTCTTTCCCAATCTGTAGGTGGGCATTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCTGCTTTCTCTTCATCATT TTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATCATTTCCCCAATTGACTGACACTGGTCC +A00126:224:H7HMWDSX3:3:1108:8693:36996 Test_seq 167329 Test_seq 167702 + - UU A00126:224:H7HMWDSX3:3:1108:8693:36996161Test_seq1673292120S131M=167552374GCGATGTACTTCAGGGTAGATCTGAGATGTCATAGCTGAATATTCTTTCCCAATCTGTAGGTGGGCATTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCTGCTTTCTCTTCATCATTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:5MD:Z:32G6A4T1T68G15MC:Z:151MAS:i:106XS:i:95XA:Z:Test_seq,-213575,128M23S,7;Yt:Z:UU A00126:224:H7HMWDSX3:3:1108:8693:3699681Test_seq16755234151M=167329-374TTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATCATTTCCCCAATTGACTGACACTGGTCCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:20S131MAS:i:151XS:i:135XA:Z:Test_seq,+213336,124M2I25M,4;Yt:Z:UU 21 34 GCGATGTACTTCAGGGTAGATCTGAGATGTCATAGCTGAATATTCTTTCCCAATCTGTAGGTGGGCATTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCTGCTTTCTCTTCATCATT TTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATCATTTCCCCAATTGACTGACACTGGTCC +A00126:224:H7HMWDSX3:3:1109:6036:1517 ! 0 Test_seq 168670 - - NR A00126:224:H7HMWDSX3:3:1109:6036:151797Test_seq1682776027S124M=168520394TTAATATAGTCACTGAGGTATTTATTAACTCTTCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGGTTTTCCATGTATACGATCATGTCATCTGCAAATAGCGATAGCTTTACTTCCTCTTTACCAATCTGGATACFFFFFFFFF,:FFFFFFFFFF:FFFFFFFFFFFF,FF,:FFFFFFFFFFFF:FFFFFFFF:FFFFFFFF:FFF:F:FF:FFFFFFFFFFF,FFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFF,:FFFF:FFF,FFFFFFFFF::FFFFNM:i:5MD:Z:69G18A0T17C2G13MC:Z:151MAS:i:99XS:i:67Yt:Z:NR A00126:224:H7HMWDSX3:3:1109:6036:1517145Test_seq16852060151M=168277-394TTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTATTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAFFFF:F:FF:FFF:FFF:FFFFFFFFFFFFF,FFFFFFFFFFFFFFFF:FFFFFFFF,FFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:112G38MC:Z:27S124MAS:i:146XS:i:61Yt:Z:NR 0 60 TTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTATTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATA +A00126:224:H7HMWDSX3:3:1109:25039:2895 Test_seq 171148 Test_seq 171486 + - UU A00126:224:H7HMWDSX3:3:1109:25039:2895161Test_seq17114860151M=171336339TGAGTAGTCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTATCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF,F:FFFF:FFFFFFFFFFFF::FFFF:F:FFF::FFFFFFF:FFFFFFFFFFFF::FFFF:FFF:NM:i:1MD:Z:84G66MC:Z:151MAS:i:146XS:i:39Yt:Z:UU A00126:224:H7HMWDSX3:3:1109:25039:289581Test_seq17133660151M=171148-339CTTTCATGATGTCAGGGAAGTTTTGTGTCAGGAGTTCTTCACCTATTTTCTCTGTGTTTTCTCTCCTTCCTCCCTGTTCTGGGTCTCCAATCACTTGCAAGTTATCCTTCTTGATAGAGTCCCACATGATTCTTAGGGTTTCTTCATTTTTFFFFFFFFFFFFFFFFFFFF,FFFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFNM:i:8MD:Z:41A20G1G1C0C12T2A11C55MC:Z:151MAS:i:111XS:i:48Yt:Z:UU 60 60 TGAGTAGTCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTATCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAAT CTTTCATGATGTCAGGGAAGTTTTGTGTCAGGAGTTCTTCACCTATTTTCTCTGTGTTTTCTCTCCTTCCTCCCTGTTCTGGGTCTCCAATCACTTGCAAGTTATCCTTCTTGATAGAGTCCCACATGATTCTTAGGGTTTCTTCATTTTT +A00126:224:H7HMWDSX3:3:1109:20627:4805 Test_seq 116647 Test_seq 395961 + - UU A00126:224:H7HMWDSX3:3:1109:20627:4805161Test_seq1166471266M=395896279315CCATAATTTGTTATGATCCACACAGTCGAATGCCTTTGCATAGTCAGTAAAACACAGGTAAACATCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF,NM:i:1MD:Z:28G37MC:Z:66MAS:i:61XS:i:56XA:Z:Test_seq,-384014,66M,2;Test_seq,+15119,66M,3;Test_seq,+395896,66M,3;Test_seq,+316208,51M2I13M,3;Yt:Z:UU A00126:224:H7HMWDSX3:3:1109:20627:480581Test_seq3958961066M=116647-279315CCATAATTTGTTATGATCCACACAGTCGAATACCTTTGTATAGTCAATAAAACACAGGTAAACATTFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:3MD:Z:27A10C26C0MC:Z:66MAS:i:55XS:i:50XA:Z:Test_seq,+384014,66M,4;Test_seq,-47037,3S63M,4;Test_seq,-316208,51M2I13M,5;Test_seq,-15119,66M,5;Test_seq,-116647,66M,5;Yt:Z:UU 12 10 CCATAATTTGTTATGATCCACACAGTCGAATGCCTTTGCATAGTCAGTAAAACACAGGTAAACATC CCATAATTTGTTATGATCCACACAGTCGAATACCTTTGTATAGTCAATAAAACACAGGTAAACATT +A00126:224:H7HMWDSX3:3:1109:27425:6778 Test_seq 167324 Test_seq 167731 + - UU A00126:224:H7HMWDSX3:3:1109:27425:677897Test_seq1673246151M=167581408GCTGGTCGGAGATGTCATAGCTGAATATTCTTTCCCAAACTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGACCTCCCAGTGATCTGCTTTCTCTTCATCATTTTTGGTAATGTTTTGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FF:FFFFFFFF:FFFFFFFFFF::FFF:F,FFFFFFFFFFFF:FFFFFFFF:,,::FF,,FF:FFFFF:FFFFFF:FFFFF:FF:F,FFNM:i:7MD:Z:7T29G0T5A60G8T5G30MC:Z:151MAS:i:116XS:i:111XA:Z:Test_seq,-213560,151M,8;Yt:Z:UU A00126:224:H7HMWDSX3:3:1109:27425:6778145Test_seq1675817151M=167324-408TTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATCATTTCCCCAATTGACTGACACTGGTCCTTTGTCAAATATCAGCTGCTCATACATGGFFFF:FFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:145T5MC:Z:151MAS:i:146XS:i:141XA:Z:Test_seq,+213307,151M,2;Test_seq,+40976,151M,7;Yt:Z:UU 6 7 GCTGGTCGGAGATGTCATAGCTGAATATTCTTTCCCAAACTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGACCTCCCAGTGATCTGCTTTCTCTTCATCATTTTTGGTAATGTTTTG TTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATCATTTCCCCAATTGACTGACACTGGTCCTTTGTCAAATATCAGCTGCTCATACATGG +A00126:224:H7HMWDSX3:3:1109:11858:6809 ! 0 Test_seq 168496 - + NR A00126:224:H7HMWDSX3:3:1109:11858:6809145Test_seq16868560126M25S=168496-315TTGTCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTACTCCCCAGTACAACTCTCTGCTAAFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:2MD:Z:2A28T94MC:Z:151MAS:i:118XS:i:58Yt:Z:NR A00126:224:H7HMWDSX3:3:1109:11858:680997Test_seq16849660151M=168685315GTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGGACTTTGTCAAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:::FFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:140A10MC:Z:126M25SAS:i:146XS:i:91Yt:Z:NR 0 60 GTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGGACTTTGTCAA +A00126:224:H7HMWDSX3:3:1109:3052:8061 ! 0 Test_seq 168720 - - NR A00126:224:H7HMWDSX3:3:1109:3052:8061161Test_seq1683476025S126M=168570374AAATATTGCCAAATAATCAGATCGATCATGTCATCAGCAAATAGTGATAGCTTTACTTCCTCCTTGCCAATCTGGATACCCTTTATTTCTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGTACGATGATGAATAAGAGTGGTGATA:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF,FFFFFFFF,FF,FFFFFFFFFFFFFFFFF,FFFFFFFFFFFFF,FFFFFFFFFFF:,FFFFFF,FF,FFFF:FFF,F:F:FFF:FFFF,,FF,FFF,::FFFFFFFF::FFFF:FNM:i:4MD:Z:10T7A88T10C7MC:Z:151MAS:i:106XS:i:56Yt:Z:NR A00126:224:H7HMWDSX3:3:1109:3052:806181Test_seq16857060151M=168347-374TATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGATTACATTAFFFFFFFFFFF,FF:FFFFFFFFF:FFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFFF:FFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:146T4MC:Z:25S126MAS:i:146XS:i:74Yt:Z:NR 0 60 TATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGATTACATTA +A00126:224:H7HMWDSX3:3:1109:31213:9298 Test_seq 171470 Test_seq 171785 + - UU A00126:224:H7HMWDSX3:3:1109:31213:9298161Test_seq17147060151M=171635316AGGGTTTCTTCATTTTTTTTAATTCTTTTATCTGATTTTTTTTCAGCTATGTTGGAGTTGATTCCCTGGTCCTCCAGAAGTCCCAGTCTACATTCTAATTGCTCGAGTCTGCTCCTCTGACTTTCTATTGCGTTGTCTAATTCTGTAATTTFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF,FFFFFFFFFFFFFFFFFNM:i:1MD:Z:113T37MC:Z:151MAS:i:146XS:i:34Yt:Z:UU A00126:224:H7HMWDSX3:3:1109:31213:929881Test_seq17163560151M=171470-316TGGATTTCTACATGCTGACTCTCTATGGATTCTTGCAACTTATTAATTTTTCCGCTATGTTCTTGAATAACCTTTTTGAGTTCTTCAACAGTTTTATCAGTGTGTTCCTTGGCTTTTTCTGCAGTTATCCTAATTTCATTTGTGATATCTTFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF,FFFF,FFFFFFFFFFFNM:i:4MD:Z:17T35A2C25T68MC:Z:151MAS:i:131XS:i:58Yt:Z:UU 60 60 AGGGTTTCTTCATTTTTTTTAATTCTTTTATCTGATTTTTTTTCAGCTATGTTGGAGTTGATTCCCTGGTCCTCCAGAAGTCCCAGTCTACATTCTAATTGCTCGAGTCTGCTCCTCTGACTTTCTATTGCGTTGTCTAATTCTGTAATTT TGGATTTCTACATGCTGACTCTCTATGGATTCTTGCAACTTATTAATTTTTCCGCTATGTTCTTGAATAACCTTTTTGAGTTCTTCAACAGTTTTATCAGTGTGTTCCTTGGCTTTTTCTGCAGTTATCCTAATTTCATTTGTGATATCTT +A00126:224:H7HMWDSX3:3:1109:20907:9392 Test_seq 123707 Test_seq 124094 + - UU A00126:224:H7HMWDSX3:3:1109:20907:9392161Test_seq12370760151M=123962388TAGGAACCCTATGGGGTAGTTCCACTGTGTCCTGTGGGGTCACTATGATTCAGAAGAGACTCGACACCACTGGTTTTGATTTTTTGGTAGTTACCCTCAGTCCCAGGGTGGTGTAAATGGTTAAGCACTTGGCTGCTAACTGAAAAGTTGGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:F,,FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFNM:i:0MD:Z:151MC:Z:133M18SAS:i:151XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1109:20907:939281Test_seq12396260133M18S=123707-388GGATCACCGTGGGTTGGAATCCACTCAGTGACAACTGGCGTTTGTGCTAGTCACCTCGAGAAGAGTCTGTTACTCCTTGTCCTTAGCTGGGTCAGATAACCATGGCCAGGTGTTGACTGATACGTAAAGTTAATCTGGAATGTACGAAGTAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:133MC:Z:151MAS:i:133XS:i:0Yt:Z:UU 60 60 TAGGAACCCTATGGGGTAGTTCCACTGTGTCCTGTGGGGTCACTATGATTCAGAAGAGACTCGACACCACTGGTTTTGATTTTTTGGTAGTTACCCTCAGTCCCAGGGTGGTGTAAATGGTTAAGCACTTGGCTGCTAACTGAAAAGTTGG GGATCACCGTGGGTTGGAATCCACTCAGTGACAACTGGCGTTTGTGCTAGTCACCTCGAGAAGAGTCTGTTACTCCTTGTCCTTAGCTGGGTCAGATAACCATGGCCAGGTGTTGACTGATACGTAAAGTTAATCTGGAATGTACGAAGTA +A00126:224:H7HMWDSX3:3:1109:16369:10802 Test_seq 214055 Test_seq 214344 + - UU A00126:224:H7HMWDSX3:3:1109:16369:1080297Test_seq21405560151M=214214290CAGTTAGAAATAGAACTACCATACAACCCAGAAATCCCACTCCTCGGAATATACCCTAGAGAAATAAGAGCCTTCACACAAACAGATATATGCACACCCATGTTTATTGCAGCTCTGTTTACAATAGCAAAAAGCTGGAAGCAACCAAGGTFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFF:FFFFFFFFNM:i:1MD:Z:0A150MC:Z:131M20SAS:i:150XS:i:119Yt:Z:UU A00126:224:H7HMWDSX3:3:1109:16369:10802145Test_seq2142141131M20S=214055-290ATGGATTAATGGATAAATAAATTGTGGTATATTCACACAATGGAATACTACACATCGATAAAGAACAGTGACGAATCTGTGAAACATTTCATAACATGGAGGAACCTGGAAGGCATTATGCTGAGCGAAATGAGTAAACTCATTAGAATGGF::,F:,FFFF:FFFFF:FFFFFFF:F:FFFFFFFFFFFF,,:FFFFF:FFFFFFFFF:FFF:FFFF:::FFFFFFFFF,FFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFNM:i:6MD:Z:6G5T37T0G4A68T5MC:Z:151MAS:i:101XS:i:98XA:Z:Test_seq,+166686,16S135M,8;Test_seq,+48661,26S125M,6;Yt:Z:UU 60 1 CAGTTAGAAATAGAACTACCATACAACCCAGAAATCCCACTCCTCGGAATATACCCTAGAGAAATAAGAGCCTTCACACAAACAGATATATGCACACCCATGTTTATTGCAGCTCTGTTTACAATAGCAAAAAGCTGGAAGCAACCAAGGT ATGGATTAATGGATAAATAAATTGTGGTATATTCACACAATGGAATACTACACATCGATAAAGAACAGTGACGAATCTGTGAAACATTTCATAACATGGAGGAACCTGGAAGGCATTATGCTGAGCGAAATGAGTAAACTCATTAGAATGG +A00126:224:H7HMWDSX3:3:1109:5321:13714 Test_seq 171138 Test_seq 171577 + - UU A00126:224:H7HMWDSX3:3:1109:5321:1371497Test_seq17113860151M=171427440TGGTTTCTGCTGAGTAGTCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATGATATGTCTTGGTGTTTTTCTTTTTGGATCFFFFFFFFFFFFFFFFFFFFFFF,:FFF:FFFFFFFFFFFFFF:FFFFFFFF,F,::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF,F,:,FFFFFFF:FFFFFFFFFFFFFFFFF:FFF:FFFFFF:FFF,FFFFFFFFFNM:i:1MD:Z:121A29MC:Z:151MAS:i:146XS:i:60Yt:Z:UU A00126:224:H7HMWDSX3:3:1109:5321:13714145Test_seq17142760151M=171138-440CACTCGCAAGTTATCCTTCTTGATAGAGTCCCACATGATTCTTAGGGTTTCTTCATTTTTTTTAATTCTTTTATCTGATTTTTTTTCAGCTATGTTGGTGTTGTTTCCGTGGTCCTCCAGAAGTCCCAGTCTACATTCTAATTGCTCGAGT,FFFFFFFFFFFFFF,FFFFFFFFFFFFF,FFFFFFFF:FFFF,:F,FFF:FFFFFFFFFFFFFFFF,FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFF:FFFFFFFFFF:FFFFFFF,FFFFFFFFFFFFFFF:FFFFNM:i:3MD:Z:98A4A4C42MC:Z:151MAS:i:136XS:i:43Yt:Z:UU 60 60 TGGTTTCTGCTGAGTAGTCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATGATATGTCTTGGTGTTTTTCTTTTTGGATC CACTCGCAAGTTATCCTTCTTGATAGAGTCCCACATGATTCTTAGGGTTTCTTCATTTTTTTTAATTCTTTTATCTGATTTTTTTTCAGCTATGTTGGTGTTGTTTCCGTGGTCCTCCAGAAGTCCCAGTCTACATTCTAATTGCTCGAGT +A00126:224:H7HMWDSX3:3:1109:8666:14121 Test_seq 171003 Test_seq 171272 + - UU A00126:224:H7HMWDSX3:3:1109:8666:1412197Test_seq17100360151M=171122270ACTTGTGTTTATCTGTAAATATCTTAATTTCTCCTTCATATTTCAAAGAGAGTTTTGCTGGATATATGATCCTTGGTTGGCAGTTTTTCTCCTTCAGTGCTCTGTATATGTCGTCCCATTCCCTTCTTGCCTGCATGGTTTCTGCTGAGTAFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFF:FFFNM:i:4MD:Z:44C0G42A19C42MC:Z:151MAS:i:131XS:i:79Yt:Z:UU A00126:224:H7HMWDSX3:3:1109:8666:14121145Test_seq17112260151M=171003-270TCCCTTCTTGCCTGCATGGTTTCTGCTGAGTAGTCTGAACTTATTCTTATAGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:50T100MC:Z:151MAS:i:146XS:i:68Yt:Z:UU 60 60 ACTTGTGTTTATCTGTAAATATCTTAATTTCTCCTTCATATTTCAAAGAGAGTTTTGCTGGATATATGATCCTTGGTTGGCAGTTTTTCTCCTTCAGTGCTCTGTATATGTCGTCCCATTCCCTTCTTGCCTGCATGGTTTCTGCTGAGTA TCCCTTCTTGCCTGCATGGTTTCTGCTGAGTAGTCTGAACTTATTCTTATAGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTG +A00126:224:H7HMWDSX3:3:1109:8178:14873 Test_seq 168739 Test_seq 169068 + - UU A00126:224:H7HMWDSX3:3:1109:8178:1487397Test_seq16873960151M=168918330AACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTCTATTGGCTAGAATTTTGTTGAGGATTTTTGCATCTATGTTCATGAGGGATATAGGTCTAAAATTTTCTTTTTTTGTAAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF,FFF:,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFF:FFFFF:FFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:81Yt:Z:UU A00126:224:H7HMWDSX3:3:1109:8178:14873145Test_seq16891860151M=168739-330GATGGTGGCTTCATAGAATGAGTTGGGTAGTATTCCGTCTTTTACTATGCTTTGAAATACCTTCAGTAGTAGTGGTGTTAGGTCTTCTCTAAAGGTTTGGTAGAACTCTGCAGTGAAGCCGTCTGGGCCAGGACTTTTTTTTGTTGGAAGTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:4MD:Z:41G1T36A9G60MC:Z:151MAS:i:131XS:i:66Yt:Z:UU 60 60 AACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTCTATTGGCTAGAATTTTGTTGAGGATTTTTGCATCTATGTTCATGAGGGATATAGGTCTAAAATTTTCTTTTTTTGTAA GATGGTGGCTTCATAGAATGAGTTGGGTAGTATTCCGTCTTTTACTATGCTTTGAAATACCTTCAGTAGTAGTGGTGTTAGGTCTTCTCTAAAGGTTTGGTAGAACTCTGCAGTGAAGCCGTCTGGGCCAGGACTTTTTTTTGTTGGAAGT +A00126:224:H7HMWDSX3:3:1109:21784:15233 Test_seq 363608 Test_seq 363964 + - UU A00126:224:H7HMWDSX3:3:1109:21784:15233161Test_seq36360860151M=363814357TAATAACACCAATAATATTCCAAAACACATATCCATATTTAGAAGTTAGATATCCATTATCAGCTTAATCAGTTCTATGTAATTCATTCTTGCTTCTCCCTCATTACCTTGATTTCATGAAATCATCTGTTTCTTGACAAAAGAGTTTTGG,FFFFFFFFFFFFF::F:FFFFFF::,:F:F:,FF:F:,FFFFFF,FF:F,,FF:F::FF:FFFFF:F,FFF,F:FFFF:F::,FFFFFF::FF:FF,F:F,FF,FFF:F:FF:,,F,FFFFFFF,,F,,,FFF,,F:,FF::F,F,FF,:NM:i:4MD:Z:45G5T82G3T12MC:Z:151MAS:i:131XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1109:21784:1523381Test_seq36381460151M=363608-357GAAAGCCTGATGCCAGAAGTCTATTCTTTGAAATAGCAAGAGTTAAGAGTACCTGGTAAAGCTCTACCCACCAGCCTCTGTGACTGTCTTTCTTTGTTAAAGGTACATTCTGGTCTGCATTTTGCAGCGAGTCTTCAGGTAAGCAGGAGAG:F,FF,FFF:F:F,:FFF,FF:,FF,FFFFF,FFFF:,F,FFFF::F:,FF:FFFFFFFF:FFFFFF:F,::F,FFF:FF:FFFFF::FFF:FFFFF,FF:FFF,FFFFFF,FFFFFF::FF:FFF,FFF:FFFFFFFFF:F,FFFFF:F,NM:i:1MD:Z:22C128MC:Z:151MAS:i:146XS:i:0Yt:Z:UU 60 60 TAATAACACCAATAATATTCCAAAACACATATCCATATTTAGAAGTTAGATATCCATTATCAGCTTAATCAGTTCTATGTAATTCATTCTTGCTTCTCCCTCATTACCTTGATTTCATGAAATCATCTGTTTCTTGACAAAAGAGTTTTGG GAAAGCCTGATGCCAGAAGTCTATTCTTTGAAATAGCAAGAGTTAAGAGTACCTGGTAAAGCTCTACCCACCAGCCTCTGTGACTGTCTTTCTTTGTTAAAGGTACATTCTGGTCTGCATTTTGCAGCGAGTCTTCAGGTAAGCAGGAGAG +A00126:224:H7HMWDSX3:3:1109:12816:15640 ! 0 Test_seq 166376 - + NR A00126:224:H7HMWDSX3:3:1109:12816:15640145Test_seq16651460130M21S=166376-268TTTCCCTTCACCCAAAGCAGTTCTTATCTACTGATTGATCAATAAAAAACCCTCTCCCTCCCTCCCTTCCTCCCCCCTTTGTAACCACAAAAGTATGTGTTCTTCTCCGTTTTTTCTATTTCTCAAGATCGATCAGGAACCAGACAAGGATFFFFFF,FFFFFFFFFFFFFFFFFF:FFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:2MD:Z:67C56C5MC:Z:151MAS:i:120XS:i:0Yt:Z:NR A00126:224:H7HMWDSX3:3:1109:12816:1564097Test_seq16637660151M=166514268TTAGAAGTCTCCTCAAACCATGTTCCCCAGACCCCAGCCCCTGCTCCACTGACCTTTGAAGCTTTCATTTTATCCCGGAAACCTCTTTGCTTTTAGTCCAGTCCAATTAGGCTGACCTTCCTTGTATTGTGTGTTGTCTTTCCCTTCACCCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFF:::F,FFFF:F:FF:FFFNM:i:1MD:Z:47G103MC:Z:130M21SAS:i:146XS:i:30Yt:Z:NR 0 60 TTAGAAGTCTCCTCAAACCATGTTCCCCAGACCCCAGCCCCTGCTCCACTGACCTTTGAAGCTTTCATTTTATCCCGGAAACCTCTTTGCTTTTAGTCCAGTCCAATTAGGCTGACCTTCCTTGTATTGTGTGTTGTCTTTCCCTTCACCC +A00126:224:H7HMWDSX3:3:1109:24198:16438 ! 0 Test_seq 137089 - - MU A00126:224:H7HMWDSX3:3:1109:24198:1643897Test_seq46648062M7S=13702190442CCAGTTGTGAGGATTTTTGTTTTCTTTATGCTGAGGTATAATCCATACTGAAGGCTGTGGTCATTAATCFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFNM:i:2MD:Z:8A28G24MC:Z:69MAS:i:52XS:i:52Yt:Z:MU A00126:224:H7HMWDSX3:3:1109:24198:16438145Test_seq137021869M=46648-90442CCAGTTGTGAGGATTTTTGTTTTCTTTATGATGAGGTGTAATCCATACTGAAGGCTGTGGTCTTTGATCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:30T38MC:Z:62M7SAS:i:64XS:i:59XA:Z:Test_seq,-315822,69M,2;Test_seq,-395506,69M,2;Test_seq,-333097,69M,3;Test_seq,-46648,69M,4;Test_seq,-8650,69M,4;Yt:Z:MU 0 8 CCAGTTGTGAGGATTTTTGTTTTCTTTATGCTGAGGTATAATCCATACTGAAGGCTGTGGTCATTAATC CCAGTTGTGAGGATTTTTGTTTTCTTTATGATGAGGTGTAATCCATACTGAAGGCTGTGGTCTTTGATC +A00126:224:H7HMWDSX3:3:1109:21965:18239 Test_seq 40140 Test_seq 168498 + + UU A00126:224:H7HMWDSX3:3:1109:21965:18239129Test_seq401405179M=168498128359CATAATAGAGGGCATTTATACAAAGCCAACAGCCAACATCATCCTAAATGGAGAGAGCCTGAAAGCAATTCCCTTGAGAFFFFFFF::FFFFFFFFFFFFFFFFFFFF:FFFFFF,FFFFF:FFFFFFFFFF:FFFFF:F:FFFFF,F:FFFFFFFFFNM:i:2MD:Z:7A59T11MC:Z:79MAS:i:69XS:i:49Yt:Z:UU A00126:224:H7HMWDSX3:3:1109:21965:1823965Test_seq1684986079M=40140-128359TCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGF,FFF::FFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFF:FF:,:FFFFFFFFFF,F,FFFFFFFFFFFFF,F:F:FFFNM:i:0MD:Z:79MC:Z:79MAS:i:79XS:i:49Yt:Z:UU 51 60 CATAATAGAGGGCATTTATACAAAGCCAACAGCCAACATCATCCTAAATGGAGAGAGCCTGAAAGCAATTCCCTTGAGA TCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATG +A00126:224:H7HMWDSX3:3:1109:32081:19257 Test_seq 170202 Test_seq 170644 + - UU A00126:224:H7HMWDSX3:3:1109:32081:19257161Test_seq17020260151M=170494443AAGTCTAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCCTCTTCTTTTTTGCTTATTGTTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATF:FFFFFFFF:FFF,FFFFFFFFF:FF:FFFFFFFFFFFFFFFFF:FFFFFF:FFFFF:FF:FFFFFFFF:FF,FFFF:FFFF:FFFFFFFFF,FF,FF,FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFNM:i:1MD:Z:60A90MC:Z:151MAS:i:146XS:i:88Yt:Z:UU A00126:224:H7HMWDSX3:3:1109:32081:1925781Test_seq17049460151M=170202-443CATTTTTCCACATGCTTTTTTGTGCTGAGACGTTTTTCTTAGTAGCTTGTGAGATCCTCATTTTCATAATGTTTAACTTTGTGTTTGTTGAGTCGTTACGTTTTTCTTGAGTTATGGAATTGATATTCCTTTTTGTGGTTACCTTATTATTFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFF,FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF,FFFF,FFNM:i:3MD:Z:13A31A34A70MC:Z:151MAS:i:136XS:i:20Yt:Z:UU 60 60 AAGTCTAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCCTCTTCTTTTTTGCTTATTGTTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGAT CATTTTTCCACATGCTTTTTTGTGCTGAGACGTTTTTCTTAGTAGCTTGTGAGATCCTCATTTTCATAATGTTTAACTTTGTGTTTGTTGAGTCGTTACGTTTTTCTTGAGTTATGGAATTGATATTCCTTTTTGTGGTTACCTTATTATT +A00126:224:H7HMWDSX3:3:1109:24975:20415 Test_seq 166044 Test_seq 166480 + - UU A00126:224:H7HMWDSX3:3:1109:24975:20415161Test_seq16604460151M=166330437TGAGAATTTGGGGTCTGTATCCCATTGGTCTCCTGCTCCCTCAGGAGTTGTCTGTTGTGCTCCCTGACAGGGCAGACATCGATTGTGGCCAGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTFFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFF,NM:i:1MD:Z:90G60MC:Z:151MAS:i:146XS:i:44Yt:Z:UU A00126:224:H7HMWDSX3:3:1109:24975:2041581Test_seq16633060151M=166044-437GTGGGATGCAGAGTGTTTTCATAATAGAATTGTTTTGCCCATTGACTTAGAAGTCCCCTCAAGCCAAGTTCCCCAGACCCCAGCCCCTGCTTCGCTGACCTTTGAAGCTTTCATTTTATCCCGGAAACCTCTTTACTTTTAATCCAGTCCAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:9MD:Z:12A18A8G14T6A3T24C42G6G9MC:Z:151MAS:i:106XS:i:27Yt:Z:UU 60 60 TGAGAATTTGGGGTCTGTATCCCATTGGTCTCCTGCTCCCTCAGGAGTTGTCTGTTGTGCTCCCTGACAGGGCAGACATCGATTGTGGCCAGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTT GTGGGATGCAGAGTGTTTTCATAATAGAATTGTTTTGCCCATTGACTTAGAAGTCCCCTCAAGCCAAGTTCCCCAGACCCCAGCCCCTGCTTCGCTGACCTTTGAAGCTTTCATTTTATCCCGGAAACCTCTTTACTTTTAATCCAGTCCA +A00126:224:H7HMWDSX3:3:1109:8169:20619 Test_seq 168399 Test_seq 168810 + - UU A00126:224:H7HMWDSX3:3:1109:8169:20619161Test_seq16839960151M=168660412ACCCTTTATTTCTTTGTCTAGCCTAATTGCCCTGGCTACGACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCCGTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFNM:i:3MD:Z:38G57T50T3MC:Z:151MAS:i:137XS:i:71Yt:Z:UU A00126:224:H7HMWDSX3:3:1109:8169:2061981Test_seq16866060151M=168399-412ATCTATTGATAAGATCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFNM:i:1MD:Z:56T94MC:Z:151MAS:i:146XS:i:55Yt:Z:UU 60 60 ACCCTTTATTTCTTTGTCTAGCCTAATTGCCCTGGCTACGACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCCGTT ATCTATTGATAAGATCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATT +A00126:224:H7HMWDSX3:3:1109:31367:22592 Test_seq 167451 Test_seq 213168 + + UU A00126:224:H7HMWDSX3:3:1109:31367:22592129Test_seq1674515481M4I66M=21316845718TTCATCATTTTTGGTAATGTTTTGTGTTCTGTTTATGCCCTGTATTAGGGCTCCTAGGGTTGTCCCTATTTTTTCTTCCATGATCGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATGCACTTGGAGTTAGTTTTTGTGCATGGFFFFFFFFFFFFFFF:FFFF,FFFFFFFFFFFFFFFFFF:FFFFFF,F:FFFFFFFFFF:FFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:8MD:Z:25A36A0T56C26MC:Z:151MAS:i:117XS:i:87Yt:Z:UU A00126:224:H7HMWDSX3:3:1109:31367:2259265Test_seq2131687151M=167451-45718AAGAAGAAAGTGGGAGGCCTCACTCTACCTGATTTCAGAACCTATTATACAGCTACAGTAGTCAAAACAGCCTGGTACTGGTACAACAACAGGCACATAGACCAATGGAACAGAATTGAGAACCCAGATATAAATCCATCCATGTATGAGCF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFF:FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:144A6MC:Z:81M4I66MAS:i:146XS:i:141XA:Z:Test_seq,-167720,151M,2;Yt:Z:UU 54 7 TTCATCATTTTTGGTAATGTTTTGTGTTCTGTTTATGCCCTGTATTAGGGCTCCTAGGGTTGTCCCTATTTTTTCTTCCATGATCGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATGCACTTGGAGTTAGTTTTTGTGCATGG AAGAAGAAAGTGGGAGGCCTCACTCTACCTGATTTCAGAACCTATTATACAGCTACAGTAGTCAAAACAGCCTGGTACTGGTACAACAACAGGCACATAGACCAATGGAACAGAATTGAGAACCCAGATATAAATCCATCCATGTATGAGC +A00126:224:H7HMWDSX3:3:1109:14751:22748 Test_seq 171454 Test_seq 171939 + - UU A00126:224:H7HMWDSX3:3:1109:14751:2274897Test_seq1714546028M1I122M=171789486GTCCCACATGATTCTTAGGGTTTCTTCATTTTTTTTTAATTCTTTTATCTGATTTTTTTTCAGCTATGTTGGTGTCGATTCCCTGGTCCTCCAGATGTCCCAGTCTACATTCTAATTGCTCGAGTCTGCTCCTCTGACTTCCTATTGCGTTFFFFFFFFFF,FFF,:FFFFFFFF:FFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FF:FFFFFFFFFFF:FF:FFFFFFFFF:FFFFFFF:F,FFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF,FNM:i:6MD:Z:71A2T19A34T9T10MC:Z:151MAS:i:118XS:i:32Yt:Z:UU A00126:224:H7HMWDSX3:3:1109:14751:22748145Test_seq17178960151M=171454-486GCATTCTGTAAATTAGTTTTTTATATTCTGTATCTGATAATTCCAGGATTGTATCTTCATTTGGGAAAGATTTTGATTCTTTTGTTTGGGGGGTTGGAGAAGCTGTCATGGTCTGTTTCTTTATGTGGTTTGATATGGACTGCTGTCTCTG:FFF::FFFFFFFFF:FFFFFF,F,:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFF,FFFFFFFF:FFFF:FFFFFF:FFFFFFFFFFFFFFFFF:,FFF:FFFFFFFFFFFFNM:i:1MD:Z:149C1MC:Z:28M1I122MAS:i:149XS:i:0Yt:Z:UU 60 60 GTCCCACATGATTCTTAGGGTTTCTTCATTTTTTTTTAATTCTTTTATCTGATTTTTTTTCAGCTATGTTGGTGTCGATTCCCTGGTCCTCCAGATGTCCCAGTCTACATTCTAATTGCTCGAGTCTGCTCCTCTGACTTCCTATTGCGTT GCATTCTGTAAATTAGTTTTTTATATTCTGTATCTGATAATTCCAGGATTGTATCTTCATTTGGGAAAGATTTTGATTCTTTTGTTTGGGGGGTTGGAGAAGCTGTCATGGTCTGTTTCTTTATGTGGTTTGATATGGACTGCTGTCTCTG +A00126:224:H7HMWDSX3:3:1109:32579:24314 Test_seq 132878 Test_seq 133237 + - UU A00126:224:H7HMWDSX3:3:1109:32579:2431497Test_seq13287860151M=133104360GACGAGCCTTGAACGTGTGAGGCTCAGTACTGCTGTGTCTTTCACGTGCGCCGCTGTATTTGTGGCCTCTGTCTCCAGGGGAAGACTGTATTCGCAGCCCCTCATTCTCAGGGTACAACATTTTGTCTTCCGAGCAGCTGGATGTGAAGGCFFFFFFFFF:FFFFFFFFFFFFF,FFFFFF:FFFFFFFF,FFFFFF:FFFFFFFFFF,F:FFFFFFF,FFF,F:FFFFFFFF,FFF:FF:,FFFFFFFFFFFFFF:FFFFFFF:,F,,FF:F:FFFF:FFFFFFF:FF:FFF,FFF,FFFFNM:i:0MD:Z:151MC:Z:134M17SAS:i:151XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1109:32579:24314145Test_seq13310460134M17S=132878-360TCAAACACTTATACACCAAGTCCTCACTTATCAACATGTTTAGGTTCCAAAGTCTAGGTTGTTATACAAAAATCGTCATTAAGCAAAAATGGTAGATGACTACAACATTACATAACTGCCAGACCACTAAGAATTAAAGTGACAGCTTGAAFFFFFFFFF:F::F,FF:F,FFFFF:F:,FFF::F::FF,,FFF,FFFF,:FFF:FFFFFFF:FFFFFFF:FFFFFFFF:,FFFF:FFFFFFFFFF,FFFF,F:FFF::FF:FFFFFFFFFFFFFFFFFFFFFF:F:FFFF:F,FF:F:FFNM:i:1MD:Z:19T114MC:Z:151MAS:i:129XS:i:0Yt:Z:UU 60 60 GACGAGCCTTGAACGTGTGAGGCTCAGTACTGCTGTGTCTTTCACGTGCGCCGCTGTATTTGTGGCCTCTGTCTCCAGGGGAAGACTGTATTCGCAGCCCCTCATTCTCAGGGTACAACATTTTGTCTTCCGAGCAGCTGGATGTGAAGGC TCAAACACTTATACACCAAGTCCTCACTTATCAACATGTTTAGGTTCCAAAGTCTAGGTTGTTATACAAAAATCGTCATTAAGCAAAAATGGTAGATGACTACAACATTACATAACTGCCAGACCACTAAGAATTAAAGTGACAGCTTGAA +A00126:224:H7HMWDSX3:3:1109:17246:27352 Test_seq 81985 Test_seq 97460 + - RU A00126:224:H7HMWDSX3:3:1109:17246:2735297Test_seq8198560151M=9744015476TTTTTGTTATTGTGAACAATGCAGTGAACATTGGTGTGCATATGTCTGTTTGTGTGACGGCTCTTATTTCTCTAGGATATATTTCTAGGAATGGGATTGCTGGATCATACGGTATTTGTATTTGTAGCTTTTTAAGGAAGCACCATATCATFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFF:F,FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:130S21MAS:i:151XS:i:81Yt:Z:RU A00126:224:H7HMWDSX3:3:1109:17246:273522193Test_seq8218460132M19H=81985-331GTTCCTGTCTCCTCGCAGCCTCTCCAGCATTTGTTATTTTCTGTTTTTTTTTGTGTCATGCCAGTAATGTCAGGATGAGATGGTATCTCATTGTGGTTTTGATTTGCATTTCTCTTAATGACTAGTGATCGAFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:132MC:Z:151MAS:i:132XS:i:21SA:Z:Test_seq,97440,-,130S21M,12,0;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1109:17246:27352145Test_seq9744012130S21M=81985-15476GTTCCTGTCTCCTCGCAGCCTCTCCAGCATTTGTTATTTTCTGTTTTTTTTTGTGTCATGCCAGTAATGTCAGGATGAGATGGTATCTCATTGTGGTTTTGATTTGCATTTCTCTTAATGACTAGTGATCGATCCAGTTCTTTTAGAAGCCFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFNM:i:0MD:Z:21MC:Z:151MAS:i:21XS:i:0SA:Z:Test_seq,82184,-,132M19S,60,0;Yt:Z:RU 60 12 TTTTTGTTATTGTGAACAATGCAGTGAACATTGGTGTGCATATGTCTGTTTGTGTGACGGCTCTTATTTCTCTAGGATATATTTCTAGGAATGGGATTGCTGGATCATACGGTATTTGTATTTGTAGCTTTTTAAGGAAGCACCATATCAT GTTCCTGTCTCCTCGCAGCCTCTCCAGCATTTGTTATTTTCTGTTTTTTTTTGTGTCATGCCAGTAATGTCAGGATGAGATGGTATCTCATTGTGGTTTTGATTTGCATTTCTCTTAATGACTAGTGATCGATCCAGTTCTTTTAGAAGCC +A00126:224:H7HMWDSX3:3:1109:17788:27821 Test_seq 168737 Test_seq 169220 + - UU A00126:224:H7HMWDSX3:3:1109:17788:27821161Test_seq16873760151M=169070484TAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGACCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTCTATTGGCTAGAATTTTGTTGAGGATTTTTGCATCTATGTTCATGAGGGATATAGGTCTAAAATTTTCTTTTTTTGTFFF,FFFFFFFFFFFF:FFFFFFF:FFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:F,FFFFFFFFFFFF:FFFFFFF,FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FF,F,FFF:FFFFF::FFFNM:i:1MD:Z:38T112MC:Z:151MAS:i:146XS:i:81Yt:Z:UU A00126:224:H7HMWDSX3:3:1109:17788:2782181Test_seq16907060151M=168737-484TTTTGATTACCGTTTCAATCTCTTTTTTTGTTATGGGTCTATTTAGTTGTTCTACTTCTGAATGTGTTAGTTTAGGTAGGTAGTATTGTTCCAAGAATTTATCCATTTCTTCTAGGTTTTCAAATTTGTTAGAGTACAGTTTTACATAGTAFFFFFFFF::,FFFFFFFFFFFFFFF:FFFFFFFF,:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFNM:i:7MD:Z:84G2T3G7C31C6A6G5MC:Z:151MAS:i:116XS:i:66Yt:Z:UU 60 60 TAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGACCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTCTATTGGCTAGAATTTTGTTGAGGATTTTTGCATCTATGTTCATGAGGGATATAGGTCTAAAATTTTCTTTTTTTGT TTTTGATTACCGTTTCAATCTCTTTTTTTGTTATGGGTCTATTTAGTTGTTCTACTTCTGAATGTGTTAGTTTAGGTAGGTAGTATTGTTCCAAGAATTTATCCATTTCTTCTAGGTTTTCAAATTTGTTAGAGTACAGTTTTACATAGTA +A00126:224:H7HMWDSX3:3:1109:12346:31078 Test_seq 171229 Test_seq 171627 + - UU A00126:224:H7HMWDSX3:3:1109:12346:31078161Test_seq17122960151M=171476399TTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATGGGGTTCGATGAGCATCTTGGATAGATATCCTTTCGTCTTTCATCATGTCAGGGAAGTTTTGTGTCAGGAGTTCTTCAACT:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFF::FFF:FFFFFF:F:FFFFFF:FF:FFFFFFFFFFFFFNM:i:1MD:Z:114G36MC:Z:19M1D132MAS:i:146XS:i:27Yt:Z:UU A00126:224:H7HMWDSX3:3:1109:12346:3107881Test_seq1714766019M1D132M=171229-399TCTTCATTTTTTTAAATTCTTTATCTGATTTTTTTTCAGCTATGTTGGTGTTGATTCCCTGGTCCTCCAGAAGTCCCAGTCTACATTCTAATTGCTCGAGTCTGCTTCTCTGACTTTCTATTGTGTTGTCTAATTCTGTAATTTTATTGTT:FFFFFFFFF:FFFFFF:FFFFFFFFFFFFF,FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:4MD:Z:13T5^T29A74C27MC:Z:151MAS:i:129XS:i:26Yt:Z:UU 60 60 TTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATGGGGTTCGATGAGCATCTTGGATAGATATCCTTTCGTCTTTCATCATGTCAGGGAAGTTTTGTGTCAGGAGTTCTTCAACT TCTTCATTTTTTTAAATTCTTTATCTGATTTTTTTTCAGCTATGTTGGTGTTGATTCCCTGGTCCTCCAGAAGTCCCAGTCTACATTCTAATTGCTCGAGTCTGCTTCTCTGACTTTCTATTGTGTTGTCTAATTCTGTAATTTTATTGTT +A00126:224:H7HMWDSX3:3:1109:30852:31782 Test_seq 166084 Test_seq 166553 + - UU A00126:224:H7HMWDSX3:3:1109:30852:3178297Test_seq16608460151M=166403470TCAGGAGTAGTCTGTTGTGCTCCCTGACAGGGCAGACATCGATTGTGGCCGGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTGGGTCTCTGGTTCATGTGGCCCTTTCTATCTCTTGGGTTCTTAGTTGTCGTGTGACCTTGGTGTTFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFF,FF,FFF,FFFFFFFFF,:FF,FFFFFFFFFFFFFFFFFFFFFFFNM:i:4MD:Z:8T77A26G26G10MC:Z:151MAS:i:131XS:i:71Yt:Z:UU A00126:224:H7HMWDSX3:3:1109:30852:31782145Test_seq16640360151M=166084-470CAGACCCCAGCCCCTGCTCCGCTGACCTTTGAAGCTTTCATTTTATCCCGGAAACCTCTTTGCTTTTAGTCCAGTCCAATTAGGCTGACCTTCCTTGTATTGAGTGTTGTCTTTCCCTTCACCCAAAGCAGTTCTTATCTACTGATTGATCF:FFFFFF:FFFFFFFFF::FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFF,FF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:102T48MC:Z:151MAS:i:146XS:i:40Yt:Z:UU 60 60 TCAGGAGTAGTCTGTTGTGCTCCCTGACAGGGCAGACATCGATTGTGGCCGGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTGGGTCTCTGGTTCATGTGGCCCTTTCTATCTCTTGGGTTCTTAGTTGTCGTGTGACCTTGGTGTT CAGACCCCAGCCCCTGCTCCGCTGACCTTTGAAGCTTTCATTTTATCCCGGAAACCTCTTTGCTTTTAGTCCAGTCCAATTAGGCTGACCTTCCTTGTATTGAGTGTTGTCTTTCCCTTCACCCAAAGCAGTTCTTATCTACTGATTGATC +A00126:224:H7HMWDSX3:3:1109:25617:34178 Test_seq 168188 Test_seq 168653 + - UU A00126:224:H7HMWDSX3:3:1109:25617:34178161Test_seq16818811148M3S=168503466GTATTGATTTGGTTATTTCCTCTTCGGTGTTCTTTTTGTTGATGTAGAGGAATCCAAGTGATTTTTGTATGTTTATTTTATAACCTGAGACTCTGCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGGTTTTCTGT:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:FFF:FFFFFFFFFF,FFFFFFFFFFFFFF::FFFF:FFFFFFFFFNM:i:2MD:Z:83T10T53MC:Z:151MAS:i:138XS:i:131XA:Z:Test_seq,-212700,151M,4;Yt:Z:UU A00126:224:H7HMWDSX3:3:1109:25617:3417881Test_seq16850360151M=168188-466AGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGGACTTTGTCAAATGCCTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:133A17MC:Z:148M3SAS:i:146XS:i:78Yt:Z:UU 11 60 GTATTGATTTGGTTATTTCCTCTTCGGTGTTCTTTTTGTTGATGTAGAGGAATCCAAGTGATTTTTGTATGTTTATTTTATAACCTGAGACTCTGCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGGTTTTCTGT AGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGGACTTTGTCAAATGCCTT +A00126:224:H7HMWDSX3:3:1109:11234:36667 Test_seq 151130 Test_seq 214124 + + UU A00126:224:H7HMWDSX3:3:1109:11234:3666765Test_seq1511301351M=21412462995AAACAGTGCTGCAATAAACATGGGTGTGCATATATCTGTTCGTGTAAAGGCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFFF:FFFFNM:i:1MD:Z:41A9MC:Z:51MAS:i:46XS:i:41XA:Z:Test_seq,+400281,51M,2;Yt:Z:UU A00126:224:H7HMWDSX3:3:1109:11234:36667129Test_seq2141241351M=151130-62995GCCTTCACACAAACAGATATATGTACACCCATGTTTATTGCAGCTCTGTTTFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:23C27MC:Z:51MAS:i:46XS:i:41XA:Z:Test_seq,-166860,51M,2;Yt:Z:UU 13 13 AAACAGTGCTGCAATAAACATGGGTGTGCATATATCTGTTCGTGTAAAGGC GCCTTCACACAAACAGATATATGTACACCCATGTTTATTGCAGCTCTGTTT +A00126:224:H7HMWDSX3:3:1109:15076:36965 ! 0 Test_seq 162165 - + NR A00126:224:H7HMWDSX3:3:1109:15076:36965145Test_seq16242660123M28S=162165-384AAAACCCTATGGAAGAGTTCTTCTGTGCACACATGGGGTGGTCACGAGTCAGAATCGACTGGACTAGCAACTAACAACAGCAGGCAAGTTCGAAGGGTCCTCCTGGGTAGTTAGATGGGATCTAATGGTCTCTTGCTTTCTTCATGTCCTCFF,FFF:F:FFFF:FFFFFFFFF:FFFFFFFF:,FFFFFFFFFFFF,FFFFF:FFFFFF:FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFNM:i:0MD:Z:123MC:Z:151MAS:i:123XS:i:0Yt:Z:NR A00126:224:H7HMWDSX3:3:1109:15076:3696597Test_seq16216560151M=162426384TGGGGGGTTGTAAGTCATAAGTGGTCTGACAGAACAAAACAAAATTATTTGCATCAGATTAAAACAAAGAACAAAGTCATTAACGTTAGGTCAAAACAAAGTCGTTAACAGAGGTATGTGAAGGAGGAGGCAGGCAGAGGGAGGAGAAAAGFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:FFFFFF:FFFFF:FFFFFFFFFFFF:FFFFFFFFF:FFFF:FFFFFFF:FF:FFFFFFFFFFFFFFFFFF,FFFFFFF,FFFF:FNM:i:1MD:Z:84C66MC:Z:123M28SAS:i:146XS:i:0Yt:Z:NR 0 60 TGGGGGGTTGTAAGTCATAAGTGGTCTGACAGAACAAAACAAAATTATTTGCATCAGATTAAAACAAAGAACAAAGTCATTAACGTTAGGTCAAAACAAAGTCGTTAACAGAGGTATGTGAAGGAGGAGGCAGGCAGAGGGAGGAGAAAAG +A00126:224:H7HMWDSX3:3:1110:14624:3364 Test_seq 168277 Test_seq 168674 + - UU A00126:224:H7HMWDSX3:3:1110:14624:336497Test_seq1682774817S134M=168524398CTCTGACAAGGTGGACTACTCTTCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGGTTTTCCGTGTGTACGATCATGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCTTGCCAATCCGGATACCCTTTATTTCFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF,F:FFFFFFF:FF,FFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFF,,FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF,,FFFFNM:i:5MD:Z:60A3A4G18A28T16MC:Z:151MAS:i:109XS:i:84Yt:Z:UU A00126:224:H7HMWDSX3:3:1110:14624:3364145Test_seq16852460151M=168277-398CTCCGTTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATFFF:FFFF,FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:4A146MC:Z:17S134MAS:i:146XS:i:0Yt:Z:UU 48 60 CTCTGACAAGGTGGACTACTCTTCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGGTTTTCCGTGTGTACGATCATGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCTTGCCAATCCGGATACCCTTTATTTC CTCCGTTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGAT +A00126:224:H7HMWDSX3:3:1110:14534:3646 Test_seq 168277 Test_seq 168674 + - UU A00126:224:H7HMWDSX3:3:1110:14534:364697Test_seq1682774717S134M=168524398CTCTGACAAGGTGGACTACTCTTCCAAACTCTTCTAGTAGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGGTTTTCCGTGTGTACGATCATGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCTTGCCAATCCGGATACCCTTTATTTCFF:F:FFF:FFFFFF,FFF:FFFFFFFFF:::FFFF,FF:F,FFF,FFFFFFFFFFFFFFFFFF:FF:FFFFFFFFF:FFFF:FF,FF:FFFFFF:FFF:FFFFF,FFFFF,:FFFFFF:F,FFFFF:FFF,FF,FFFFF::FF:FFF:FFNM:i:6MD:Z:19T40A3A4G18A28T16MC:Z:151MAS:i:104XS:i:79Yt:Z:UU A00126:224:H7HMWDSX3:3:1110:14534:3646145Test_seq16852460151M=168277-398CTCCGTTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATFF:FFFFFFFF:FF:FFFF:,:FFFFF:FFF:FF:FFFFFF:FF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFNM:i:1MD:Z:4A146MC:Z:17S134MAS:i:146XS:i:0Yt:Z:UU 47 60 CTCTGACAAGGTGGACTACTCTTCCAAACTCTTCTAGTAGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGGTTTTCCGTGTGTACGATCATGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCTTGCCAATCCGGATACCCTTTATTTC CTCCGTTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGAT +A00126:224:H7HMWDSX3:3:1110:14841:4492 Test_seq 169863 Test_seq 170322 + - UU A00126:224:H7HMWDSX3:3:1110:14841:4492161Test_seq16986360151M=170172460TCCATGTGCGCTAGAAAAAAAAGTATATTTTGCAGCAGTTGGGTGGAGAGTTCTGTATAAGTCTATGAGGTCAAGTTGGTTGATTGTTGTAAGTAGGTCTTCCGTGTCTCTATTGAGCTTCTTACTGGATGTCCTGTCCTTCTCCGAAAGTFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFNM:i:3MD:Z:3C5A101G39MC:Z:151MAS:i:137XS:i:34Yt:Z:UU A00126:224:H7HMWDSX3:3:1110:14841:449281Test_seq17017260151M=169863-460GTGTCCTTCTTTATCCTTTGTGGCGGATTTAAGTCTAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCCTCTTCTTTTTTGCTTATTATTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFNM:i:1MD:Z:24A126MC:Z:151MAS:i:146XS:i:74Yt:Z:UU 60 60 TCCATGTGCGCTAGAAAAAAAAGTATATTTTGCAGCAGTTGGGTGGAGAGTTCTGTATAAGTCTATGAGGTCAAGTTGGTTGATTGTTGTAAGTAGGTCTTCCGTGTCTCTATTGAGCTTCTTACTGGATGTCCTGTCCTTCTCCGAAAGT GTGTCCTTCTTTATCCTTTGTGGCGGATTTAAGTCTAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCCTCTTCTTTTTTGCTTATTATTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTA +A00126:224:H7HMWDSX3:3:1110:3378:5181 Test_seq 167332 Test_seq 167676 + - UU A00126:224:H7HMWDSX3:3:1110:3378:5181161Test_seq1673322115S136M=167526345TATAGATTACATTTAGAGATGTCATAGCTGAATATTCTTTCCCAGTCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTCATAATTTTTGGTAAFFFFF:FFFFF,FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF::FFFFF:FFFFFFFFFFFF:FFFFF:FFFFFF,FFFFFFFFFFF,FFFFFFFFFFFFFFFFF:F:FFFFFFFF,FFFF:FFFFFFFFFFFFF,FFFFFFF:FFFNM:i:2MD:Z:36A87C11MC:Z:151MAS:i:126XS:i:116XA:Z:Test_seq,-213567,136M15S,4;Yt:Z:UU A00126:224:H7HMWDSX3:3:1110:3378:518181Test_seq16752652151M=167332-345TTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGGCTATCA,FFFFFFFF::FFFF,FFFFFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FF:FFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:144A6MC:Z:15S136MAS:i:146XS:i:121XA:Z:Test_seq,+213362,98M2I23M2I26M,6;Yt:Z:UU 21 52 TATAGATTACATTTAGAGATGTCATAGCTGAATATTCTTTCCCAGTCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTCATAATTTTTGGTAA TTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGGCTATCA +A00126:224:H7HMWDSX3:3:1110:12020:6057 ! 0 Test_seq 171156 - + NR A00126:224:H7HMWDSX3:3:1110:12020:605781Test_seq17135060121M30S=171156-315GGGAAGTTTTGTGTCAGGAGTTCTTCAACTATTTTCTCTGTGTTTTCTGTCCCCCCTCCCTGTTCTGGGACTCCAATCACTCGCAAGTTATCCTTCTTGATAGAGTCCCACATGATTCTTATGAGGATGGTGTTTCATTCTCTTGTACTCAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:2MD:Z:50G15T54MC:Z:151MAS:i:111XS:i:0Yt:Z:NR A00126:224:H7HMWDSX3:3:1110:12020:6057161Test_seq17115660151M=171350315CTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATGGGGTTCGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFFF::FFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:121M30SAS:i:151XS:i:35Yt:Z:NR 0 60 CTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATGGGGTTCG +A00126:224:H7HMWDSX3:3:1110:15130:6151 ! 0 ! 0 - - MM A00126:224:H7HMWDSX3:3:1110:15130:615165Test_seq333515062M=38398650472CCTTTGCATAGTCAATAAAACACAGGTAAACATCCTTCTGGTATTCTCTGCCTTCAGCCAGGFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFF:FFFF,F::FFFFFFFFF:FF,F,:,FNM:i:1MD:Z:51T10MC:Z:62MAS:i:57XS:i:57XA:Z:Test_seq,-383986,62M,1;Test_seq,+15151,62M,2;Test_seq,+316240,19M2I41M,3;Yt:Z:MM A00126:224:H7HMWDSX3:3:1110:15130:6151129Test_seq383986062M=333515-50472CCTGGCTGAAGGCAGAGAATACCAGAAGGATGTTTACCTGTGTTTTATTGACTATGCAAAGGFFFFFF:FFFFFFFFFFFFFFFF:FFFFF:FF:FFFFF,FFFF::FFFFFF,F:FFF:F:FFNM:i:1MD:Z:10A51MC:Z:62MAS:i:57XS:i:57XA:Z:Test_seq,-333515,62M,1;Test_seq,-15151,62M,2;Test_seq,-316240,19M2I41M,3;Test_seq,+449331,62M,4;Yt:Z:MM 0 0 CCTTTGCATAGTCAATAAAACACAGGTAAACATCCTTCTGGTATTCTCTGCCTTCAGCCAGG CCTGGCTGAAGGCAGAGAATACCAGAAGGATGTTTACCTGTGTTTTATTGACTATGCAAAGG +A00126:224:H7HMWDSX3:3:1110:18936:8046 Test_seq 168392 Test_seq 168721 + - UU A00126:224:H7HMWDSX3:3:1110:18936:8046161Test_seq16839260151M=168571330TCCGGATACCCTTTATTTCTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGCATGATGTTGAATAAGAGCGGTGATAAGGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATGTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF,FFFFFFFFFNM:i:6MD:Z:2T52T1C24A20T44A2MC:Z:151MAS:i:125XS:i:73Yt:Z:UU A00126:224:H7HMWDSX3:3:1110:18936:804681Test_seq16857160151M=168392-330ATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGATTACATTAAFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFNM:i:1MD:Z:145T5MC:Z:151MAS:i:146XS:i:73Yt:Z:UU 60 60 TCCGGATACCCTTTATTTCTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGCATGATGTTGAATAAGAGCGGTGATAAGGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATGTT ATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGATTACATTAA +A00126:224:H7HMWDSX3:3:1110:19090:8406 ! 0 Test_seq 169404 - - MR A00126:224:H7HMWDSX3:3:1110:19090:84062209Test_seq1689946022H129M=169254411GTTAAGTCTTCTCTGAAGGTTTGGTAGAACTCTGCAGTGAAGCCATCTGGGCCAGGACTTTTTTTTGTTGGAAGTTTTTTGATTACCGTTTCAATCTCTTTTTTTGTTATGGGTCTATTTAGTTGTTCTFFFFFFFFFFFFFFFFFFF,FFFFF:FF:F,FFFFFFFFFFFFFFFFFFFFFFFFF:FF,FFFFFFF,FFF:F,FF:FFFF,FFFFFFF:F:FFFF:FFFF,F,F::FF:FFFFFF,,F:FFFFFFFFFNM:i:1MD:Z:44G84MC:Z:151MAS:i:124XS:i:49SA:Z:Test_seq,52324,-,127S24M,0,0;Yt:Z:MRNEXT_SAMA00126:224:H7HMWDSX3:3:1110:19090:8406177Test_seq523240127S24M=169254117058AGAACAACTAAATAGACCCATAACAAAAAAAGAGATTGAAACGGTAATCAAAAAACTTCCAACAAAAAAAAGTCCTGGCCCAGATGGCTTCACTGCAGAGTTCTACCAAACCTTCAGAGAAGACTTAACTCTCTGTCTCTTTATTGGTGCAFFFFFFFFF:F,,FFFFFF:FF::F,F,FFFF:FFFF:F:FFFFFFF,FFFF:FF,F:FFF,FFFFFFF,FF:FFFFFFFFFFFFFFFFFFFFFFFFF,F:FF:FFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:,NM:i:0MD:Z:24MC:Z:151MAS:i:24XS:i:24SA:Z:Test_seq,168994,+,22S129M,60,1;XA:Z:Test_seq,-154647,127S24M,0;Test_seq,+255573,24M127S,0;Test_seq,+38332,24M127S,0;Test_seq,-170380,127S24M,0;Yt:Z:MR A00126:224:H7HMWDSX3:3:1110:19090:8406113Test_seq16925460151M=52324-117058TGTTGTGATGTGGTCCTTCTCGTTTCTTATTCGGGTTATTTGTTTCCTTTCCTGTTTTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFF:F:FFF:FFFFFFF:FFFFFFFF:FFFFF:F:FFFF:FFFFFFFFFF:FF:FFFFFFFFF:FFFFFFFFFFF,:FFFFFFFFFFFFFFFFFFFFFFF:FF,F:FFNM:i:1MD:Z:14T136MC:Z:127S24MAS:i:146XS:i:82Yt:Z:MR 0 60 AGAACAACTAAATAGACCCATAACAAAAAAAGAGATTGAAACGGTAATCAAAAAACTTCCAACAAAAAAAAGTCCTGGCCCAGATGGCTTCACTGCAGAGTTCTACCAAACCTTCAGAGAAGACTTAACTCTCTGTCTCTTTATTGGTGCA TGTTGTGATGTGGTCCTTCTCGTTTCTTATTCGGGTTATTTGTTTCCTTTCCTGTTTTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTG +A00126:224:H7HMWDSX3:3:1110:18765:8531 Test_seq 169027 Test_seq 169502 + - UU A00126:224:H7HMWDSX3:3:1110:18765:853197Test_seq16902760151M=169352476GCAGTGAAGCCGTCCGGGCCAGGACTTTTTTTTGTTGGGAGTTTTTTGATTACCGTTTCAATCTCTTTTTTTGTTATGGGTCTATTTAGTTGTTCTGCTTCTGAATGTGTTAGTTTAGGTAGGTAGTGTTTTTCAAGGAATTCATCCATTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F,FFFFF,FFF:FFFFFFFFFF:FFF:FFFFFF::FFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFF,F:FF:FF,FFFFF:FF,:FNM:i:5MD:Z:14T23A57A37G1A14MC:Z:151MAS:i:126XS:i:61Yt:Z:UU A00126:224:H7HMWDSX3:3:1110:18765:8531145Test_seq16935260151M=169027-476TTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGATTCTTTTGTTGCTCACTTTCTATTTGTTCAAGTTGFFFFFFFFF:FFF,FFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:115G35MC:Z:151MAS:i:146XS:i:101Yt:Z:UU 60 60 GCAGTGAAGCCGTCCGGGCCAGGACTTTTTTTTGTTGGGAGTTTTTTGATTACCGTTTCAATCTCTTTTTTTGTTATGGGTCTATTTAGTTGTTCTGCTTCTGAATGTGTTAGTTTAGGTAGGTAGTGTTTTTCAAGGAATTCATCCATTT TTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGATTCTTTTGTTGCTCACTTTCTATTTGTTCAAGTTG +A00126:224:H7HMWDSX3:3:1110:23927:10676 Test_seq 166048 Test_seq 166495 + - UU A00126:224:H7HMWDSX3:3:1110:23927:10676161Test_seq16604860151M=166345448AATTTGGGGTCTGTATCCCATTGGTCTCCTGCTCCCTCAGGAGTTGTCTGTTGTGCTCCCTGACAGGGCAGACATCGATTGTGGCCGGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCTTTTCTGTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:143C7MC:Z:151MAS:i:146XS:i:45Yt:Z:UU A00126:224:H7HMWDSX3:3:1110:23927:1067681Test_seq16634560151M=166048-448TTTTCATAATAGAATTATTTTGCCCATTGACTTAGAAGTCCCCTCAAACCAAGTTCCCCAGACCCCAGCCCCTGCTCCGCTGACCTTTGAGGCATTCATTTTATCCTGGAAACCTCTTTGCTTTTAGTCCAGTCCAATTAGGCTGACCTTCFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFNM:i:6MD:Z:25G14T10T38A2T12C44MC:Z:151MAS:i:121XS:i:0Yt:Z:UU 60 60 AATTTGGGGTCTGTATCCCATTGGTCTCCTGCTCCCTCAGGAGTTGTCTGTTGTGCTCCCTGACAGGGCAGACATCGATTGTGGCCGGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCTTTTCTGT TTTTCATAATAGAATTATTTTGCCCATTGACTTAGAAGTCCCCTCAAACCAAGTTCCCCAGACCCCAGCCCCTGCTCCGCTGACCTTTGAGGCATTCATTTTATCCTGGAAACCTCTTTGCTTTTAGTCCAGTCCAATTAGGCTGACCTTC +A00126:224:H7HMWDSX3:3:1110:29161:13416 Test_seq 169352 Test_seq 169778 + - UU A00126:224:H7HMWDSX3:3:1110:29161:1341697Test_seq16935260151M=169628427TTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGGTTCTTTTGTTGCTCACTTTCTATTTGTTCAAGTTGFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFF:FFFF:FFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFF,FFFFFFFFFFFF,FFFF:NM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:66Yt:Z:UU A00126:224:H7HMWDSX3:3:1110:29161:13416145Test_seq16962860151M=169352-427CGTTGCTTTCTAAGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTATTGTTCATTTTCCAAGTATGTGATTTCTTTTCCCTAGTTTTTCTGTTATTGATTTCTAGCTTCATTGCCTTGTGGTCTGAFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:4MD:Z:0T34G54T28C31MC:Z:151MAS:i:135XS:i:62Yt:Z:UU 60 60 TTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGGTTCTTTTGTTGCTCACTTTCTATTTGTTCAAGTTG CGTTGCTTTCTAAGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTATTGTTCATTTTCCAAGTATGTGATTTCTTTTCCCTAGTTTTTCTGTTATTGATTTCTAGCTTCATTGCCTTGTGGTCTGA +A00126:224:H7HMWDSX3:3:1110:18873:14356 Test_seq 167906 Test_seq 168398 + - UU A00126:224:H7HMWDSX3:3:1110:18873:14356161Test_seq16790660151M=168252493TTCCCTTCCATATGAAATTAGTGATTTGTTTCTCTATCCCCTTAAAATATGACATTGGTATTTGGATTGGAAGTGCGTTAAATGTATAGATGGCTTTTGGTAGAATAGACATTTTTACTATGTTAAGTCTTCCTATCCATGAGCAGGGTATFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFF,FFFFFFF:FF,FFFFF,FFFF,FFFF:FFFFFFF:FFFF:FF,F:,,FFF,F:F,:FFFFF:::FFFF:NM:i:1MD:Z:80T70MC:Z:92M4I55MAS:i:146XS:i:76Yt:Z:UU A00126:224:H7HMWDSX3:3:1110:18873:1435681Test_seq1682524192M4I55M=167906-493TTGTATGTTTATTTTATAACCTGAGACTCTGCCAAACTCTTCTATTGGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGGTTTTCCATGTATACGATCGATCATGTCATCTGCAAATAGTAATAGCTTTACTTCTTCCTTGCCAATCTGGATF:FFF:F,FFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:11MD:Z:19T10T15A47G18A1G13C17MC:Z:151MAS:i:102XS:i:77Yt:Z:UU 60 41 TTCCCTTCCATATGAAATTAGTGATTTGTTTCTCTATCCCCTTAAAATATGACATTGGTATTTGGATTGGAAGTGCGTTAAATGTATAGATGGCTTTTGGTAGAATAGACATTTTTACTATGTTAAGTCTTCCTATCCATGAGCAGGGTAT TTGTATGTTTATTTTATAACCTGAGACTCTGCCAAACTCTTCTATTGGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGGTTTTCCATGTATACGATCGATCATGTCATCTGCAAATAGTAATAGCTTTACTTCTTCCTTGCCAATCTGGAT +A00126:224:H7HMWDSX3:3:1110:29297:15468 Test_seq 168209 Test_seq 168326 + - UU A00126:224:H7HMWDSX3:3:1110:29297:1546897Test_seq16820926114M4S=168209118CTTCGGTGTTCTTTTTGTTGATGTAGAGGAATCCAAGTGATTTTTGTATGTTTATTTTATAACCTGAGACTCTTCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTAAGTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,F,FNM:i:1MD:Z:62T51MC:Z:118MAS:i:109XS:i:98XA:Z:Test_seq,-212716,4S114M,4;Yt:Z:UU A00126:224:H7HMWDSX3:3:1110:29297:15468145Test_seq16820928118M=168209-118CTTCGGTGTTCTTTTTGTTGATGTAGAGGAATCCAAGTGATTTTTGTATGTTTATTTTATAACCTGAGACTCTTCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCTTFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:62T55MC:Z:114M4SAS:i:113XS:i:101XA:Z:Test_seq,+212712,118M,5;Yt:Z:UU 26 28 CTTCGGTGTTCTTTTTGTTGATGTAGAGGAATCCAAGTGATTTTTGTATGTTTATTTTATAACCTGAGACTCTTCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTAAGT CTTCGGTGTTCTTTTTGTTGATGTAGAGGAATCCAAGTGATTTTTGTATGTTTATTTTATAACCTGAGACTCTTCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCTT +A00126:224:H7HMWDSX3:3:1110:9796:16736 Test_seq 167173 Test_seq 167481 + - UU A00126:224:H7HMWDSX3:3:1110:9796:16736161Test_seq1671732716S135M=167331309AGATGCTAGATGGATCGATCGAGAGCATTTTCTCATGTACCTGTCAGCTGCCTGAATATCTTCCTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAATCATGTAGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFF,NM:i:5MD:Z:23T4T0G17T22T64MC:Z:151MAS:i:110XS:i:96XA:Z:Test_seq,-213727,135M16S,8;Yt:Z:UU A00126:224:H7HMWDSX3:3:1110:9796:1673681Test_seq16733123151M=167173-309TGAGATGTCATAGCTGAATATTCTTTCCCAGTCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:37A113MC:Z:16S135MAS:i:146XS:i:135XA:Z:Test_seq,+213553,151M,4;Yt:Z:UU 27 23 AGATGCTAGATGGATCGATCGAGAGCATTTTCTCATGTACCTGTCAGCTGCCTGAATATCTTCCTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAATCATGTAG TGAGATGTCATAGCTGAATATTCTTTCCCAGTCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTG +A00126:224:H7HMWDSX3:3:1110:30951:17675 ! 0 Test_seq 449149 - - MU A00126:224:H7HMWDSX3:3:1110:30951:17675161Test_seq383784052M=44910865366CTGACTGGAAGAGATCCATATTTATGCCTATTCCCAAGAAAGGTGATCCAACFFFFFF:FFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFF:FF:FFFFF::FFNM:i:1MD:Z:20C31MC:Z:42M10SAS:i:47XS:i:47XA:Z:Test_seq,-333748,52M,1;Test_seq,-15384,52M,1;Test_seq,+449108,52M,2;Yt:Z:MU A00126:224:H7HMWDSX3:3:1110:30951:1767581Test_seq4491082742M10S=383784-65366CTGACTGGAAGAGATCCATATTTATGCCTATTCCCAAGAAAGCCGATGCAACFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFF:FFFFFFFFFFFNM:i:0MD:Z:42MC:Z:52MAS:i:42XS:i:37XA:Z:Test_seq,+15394,10S42M,1;Test_seq,+47309,10S39M3S,1;Yt:Z:MU 0 27 CTGACTGGAAGAGATCCATATTTATGCCTATTCCCAAGAAAGGTGATCCAAC CTGACTGGAAGAGATCCATATTTATGCCTATTCCCAAGAAAGCCGATGCAAC +A00126:224:H7HMWDSX3:3:1110:13666:17832 Test_seq 168483 Test_seq 168776 + - UU A00126:224:H7HMWDSX3:3:1110:13666:17832161Test_seq16848360151M=168630294TTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:12T138MC:Z:42M4I105MAS:i:146XS:i:93Yt:Z:UU A00126:224:H7HMWDSX3:3:1110:13666:1783281Test_seq1686306042M4I105M=168483-294GTGTTGGACTTTGTCAAATGCCTTTTCTGCATCAATTGATAAGATCGATCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGATTACAGTAATGGTTTTCCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF,FFFFFFFFFFFFFFF:FFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFNM:i:9MD:Z:6A26T52T1T10T47MC:Z:151MAS:i:112XS:i:58Yt:Z:UU 60 60 TTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGT GTGTTGGACTTTGTCAAATGCCTTTTCTGCATCAATTGATAAGATCGATCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGATTACAGTAATGGTTTTCCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATC +A00126:224:H7HMWDSX3:3:1110:2636:18176 ! 0 ! 0 - - MM A00126:224:H7HMWDSX3:3:1110:2636:1817697Test_seq167381073M=16738173CTCTTTTGGAGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTCFF:FFF:,FFF:F,FF,:F:F:F,::F:FF:::,FFFFFFFFFFF,:F,FF,FFFFF:FF:FFFFFFFFFFF:NM:i:1MD:Z:9T63MC:Z:73MAS:i:68XS:i:68XA:Z:Test_seq,-213581,73M,1;Yt:Z:MM A00126:224:H7HMWDSX3:3:1110:2636:18176145Test_seq167381073M=167381-73CTCTTTTGGAGAAGTATTTAGTTGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTCFF,FFFF:F,FFFFF,FFF:,,FFF,F:FFFFFF,FFFFFFFFFF:FFFFF,:FFFFFFF,FF:FFFFFFFF,NM:i:3MD:Z:9T5C5A51MC:Z:73MAS:i:58XS:i:58XA:Z:Test_seq,+213581,73M,3;Yt:Z:MM 0 0 CTCTTTTGGAGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTC CTCTTTTGGAGAAGTATTTAGTTGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTC +A00126:224:H7HMWDSX3:3:1110:12156:19695 Test_seq 170074 Test_seq 170357 + - UU A00126:224:H7HMWDSX3:3:1110:12156:19695161Test_seq17007460151M=170207284AAAATTTGATTTATGTATCTTGCAGCCCTGTCATTAGGTGCGTAAATATTTAATATGGTTATGTCTTCCTGATCAATTGTCCCTTTTATCATTATATAGTGTCCTTCTTTATCCTTTGTGGTGGATTTAAGTCTAAAGTCTATTTTGTCAGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF:FFFFFF,::FFFFFFFFFFFFF,F,F,NM:i:5MD:Z:33C1G26A58C0A28MC:Z:151MAS:i:126XS:i:82Yt:Z:UU A00126:224:H7HMWDSX3:3:1110:12156:1969581Test_seq17020760151M=170074-284TAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCCTCTTCTTTTTTGCTTATTGTTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATC:FFFFF:FFFFFFF:FFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FNM:i:1MD:Z:55A95MC:Z:151MAS:i:146XS:i:88Yt:Z:UU 60 60 AAAATTTGATTTATGTATCTTGCAGCCCTGTCATTAGGTGCGTAAATATTTAATATGGTTATGTCTTCCTGATCAATTGTCCCTTTTATCATTATATAGTGTCCTTCTTTATCCTTTGTGGTGGATTTAAGTCTAAAGTCTATTTTGTCAG TAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCCTCTTCTTTTTTGCTTATTGTTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATC +A00126:224:H7HMWDSX3:3:1110:1859:20588 Test_seq 228268 Test_seq 228639 + - UU A00126:224:H7HMWDSX3:3:1110:1859:2058897Test_seq22826860151M=228489372GTTTACTCAAATGGACACTCATTCCTCTAGTAATTTTTTCAAGTATGCCTCATTGCTTTTCTATAAAACAAGCGCTGAAGAACTCTCAGAGGGCTGGTGCAAGCTTTGAGCATATTAGCTCTTTATTAGAGGTCTTGAATGCCCACACAGCFFFFFFFFF:FFF:FFFFFFFFF,FFFF::FFF,FFFFFFFFFFFF:,FF:FFFFFFFFF:FFFFFF:FFF,F,FFFFFFFFFFF:FFFF:,:FF,FFFFFFFFFFFFFF:FFFFFF,:FFFFFFFFF,F,FFFFF,FFFF,:,,F:,FFFNM:i:2MD:Z:33G110C6MC:Z:151MAS:i:141XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1110:1859:20588145Test_seq22848960151M=228268-372AACCCCGATAACTGCTGTTTGAGAAGTCACTCTGGAAGAATTAAAAACAAAAACCCGTTGCTGCTGAGTTGATTCTGACTCGTAGCGACCCTACAGGACACAGTAGAAATGCCCCTATAAGGCTTCTAAGGAGTGGCTGGTGGATTCAAATFFF:,F,FF:F::F:F,FFF:FF,,:,FFFFFF::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFF,FFF:FFFFFFFFFF:F:F:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF,FFFFFNM:i:1MD:Z:26G124MC:Z:151MAS:i:146XS:i:0Yt:Z:UU 60 60 GTTTACTCAAATGGACACTCATTCCTCTAGTAATTTTTTCAAGTATGCCTCATTGCTTTTCTATAAAACAAGCGCTGAAGAACTCTCAGAGGGCTGGTGCAAGCTTTGAGCATATTAGCTCTTTATTAGAGGTCTTGAATGCCCACACAGC AACCCCGATAACTGCTGTTTGAGAAGTCACTCTGGAAGAATTAAAAACAAAAACCCGTTGCTGCTGAGTTGATTCTGACTCGTAGCGACCCTACAGGACACAGTAGAAATGCCCCTATAAGGCTTCTAAGGAGTGGCTGGTGGATTCAAAT +A00126:224:H7HMWDSX3:3:1110:31114:23375 Test_seq 168238 Test_seq 168675 + - UU A00126:224:H7HMWDSX3:3:1110:31114:23375161Test_seq16823856151M=168525438AATCCAAGTGATTTTTGTATGTTTATTTTATAACCTGAGACACTTCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGGTTTTCCATGTATACAATCATGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCTTCCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF::FFFFFFFFFFFFFNM:i:6MD:Z:33T7T65G0G18A21G1MC:Z:151MAS:i:124XS:i:94Yt:Z:UU A00126:224:H7HMWDSX3:3:1110:31114:2337581Test_seq16852560151M=168238-438TCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCTATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATCFFFFFF,FFFFFFFFF::FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFNM:i:1MD:Z:69A81MC:Z:151MAS:i:146XS:i:87Yt:Z:UU 56 60 AATCCAAGTGATTTTTGTATGTTTATTTTATAACCTGAGACACTTCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGGTTTTCCATGTATACAATCATGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCTTCC TCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCTATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATC +A00126:224:H7HMWDSX3:3:1110:14660:24721 Test_seq 171186 Test_seq 171564 + - UU A00126:224:H7HMWDSX3:3:1110:14660:2472197Test_seq17118660151M=171417379AGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTCTTTGGATCAATCTTAAATGGGGTTCGATGAGCATCTTGGATAGATATCCTTTCGTCFFFF,FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFNM:i:1MD:Z:94T56MC:Z:3S65M1D22M1I60MAS:i:146XS:i:67Yt:Z:UU A00126:224:H7HMWDSX3:3:1110:14660:24721145Test_seq171417603S65M1D22M1I60M=171186-379CCGGGACTCCAGTCACTCGCAAGTTATCCTTCTTGATAGAGTCCCACATGATTCTTAGGGTTTCTTCATTTTTTAAATTCTTTTATCTGATTTTTTTTTCAGCTATGTTGGTGTTGATTCCCTGGTCCTCCAGAAGTCCCAGTCTACATTCFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:FFF,FFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFNM:i:5MD:Z:8A56^T6T35A39MC:Z:151MAS:i:118XS:i:39Yt:Z:UU 60 60 AGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTCTTTGGATCAATCTTAAATGGGGTTCGATGAGCATCTTGGATAGATATCCTTTCGTC CCGGGACTCCAGTCACTCGCAAGTTATCCTTCTTGATAGAGTCCCACATGATTCTTAGGGTTTCTTCATTTTTTAAATTCTTTTATCTGATTTTTTTTTCAGCTATGTTGGTGTTGATTCCCTGGTCCTCCAGAAGTCCCAGTCTACATTC +A00126:224:H7HMWDSX3:3:1110:30897:25097 ! 0 Test_seq 186656 - - NR A00126:224:H7HMWDSX3:3:1110:30897:25097161Test_seq1862696022S129M=186506388ATTTATTTATAGTTCTGTGAGTGTCTCTAAAGGGTTGTGGACATTGGGGTTGCAGACTGAGCTAGTCAGTGACAGCCCACTCAACTTTTGCTCATGCAGCCCTTCTGATGCTTATTCCTTGTTTTAATAAAAAGACTTCCTACCAGAAACAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFNM:i:0MD:Z:129MC:Z:151MAS:i:129XS:i:0Yt:Z:NR A00126:224:H7HMWDSX3:3:1110:30897:2509781Test_seq18650660151M=186269-388CTTATATTTAAAAAAAAAAAAAAGAGAACCTTAGTTTTCAAAAGACACAAGACTTAGATTAGAAGTGCCTACCACATGTCTAGCATGGTGAATTAAAAACAACAACAACAACAAAAAAGGTAACTTGTGAAACTTTAGAACACCAAAGATT,FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:22S129MAS:i:151XS:i:23Yt:Z:NR 0 60 CTTATATTTAAAAAAAAAAAAAAGAGAACCTTAGTTTTCAAAAGACACAAGACTTAGATTAGAAGTGCCTACCACATGTCTAGCATGGTGAATTAAAAACAACAACAACAACAAAAAAGGTAACTTGTGAAACTTTAGAACACCAAAGATT +A00126:224:H7HMWDSX3:3:1110:2166:26412 Test_seq 156809 Test_seq 157242 + - UU A00126:224:H7HMWDSX3:3:1110:2166:2641297Test_seq15680960151M=157092434GGAGCCTGCGAACAAGGGCCTATTCTCCCGAAATAGGCCCACATAGGTCCATGCAGAAGGGAAAGGTGCTCAAAGTCCACGGACAGTTTATGCCTGGATAGGAGCTGCTTCTGTCCTGAGCTCCCCTGGTTAGTGGAACTAGCAAATTATCFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:F:FF::,FFFF,FFFFFFFFFFFFFFF:FFFFFFFF:FFFF:F:FF:FFFFFF:,:FFFFFFFFF:FF:FFFFFFFFFFFFF:FFF,FFFFFFFFF:FF:FF:FFFFFFFFF:FNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:97Yt:Z:UU A00126:224:H7HMWDSX3:3:1110:2166:26412145Test_seq15709260151M=156809-434TATGCAAGTACTTAGCTTTTGCCGAGAGCACTGTCGTTCTCAGGTTCCGGAGGTGTGAGTAGACTGTGTGGCTGGCTGCTTCTCCCTGAGGAAACTGTAGCCAAACGCTAGTACCAGCCCACCACCGCCGCTGCCGTGGGAATGGTGCCTGFFFFF:FFFFFFFFFFFFFFFF:FF,FFF:F:F:FFFFFFFFFFF:FFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF:FFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:55Yt:Z:UU 60 60 GGAGCCTGCGAACAAGGGCCTATTCTCCCGAAATAGGCCCACATAGGTCCATGCAGAAGGGAAAGGTGCTCAAAGTCCACGGACAGTTTATGCCTGGATAGGAGCTGCTTCTGTCCTGAGCTCCCCTGGTTAGTGGAACTAGCAAATTATC TATGCAAGTACTTAGCTTTTGCCGAGAGCACTGTCGTTCTCAGGTTCCGGAGGTGTGAGTAGACTGTGTGGCTGGCTGCTTCTCCCTGAGGAAACTGTAGCCAAACGCTAGTACCAGCCCACCACCGCCGCTGCCGTGGGAATGGTGCCTG +A00126:224:H7HMWDSX3:3:1110:19162:27007 ! 0 Test_seq 171120 - + NR A00126:224:H7HMWDSX3:3:1110:19162:27007145Test_seq17133160125M26S=171120-336TTCATCTTTCATGATGTCAGGGAAGTTTTGTGTAAGGAGTTCTTCAACTATTTTCTCTGTGTTTTCTGTCCCCCCTCCCTGTTCTGGGACTCCAATCACTCGCAAGTTATCCTTCTTGATAGAGTAGTCACTATTAGGGTCACTAGGGGTAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:4MD:Z:3G29C35G15T39MC:Z:151MAS:i:106XS:i:40Yt:Z:NR A00126:224:H7HMWDSX3:3:1110:19162:2700797Test_seq17112060151M=171331336ATTCTCTTCTTGCCTGCATGGTTTCTGCTGAGTAGTCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:,,,,F:FFFFFFFFFFFFFFFFFF,:FFFFF,FFFF:FFFFFFF,FFFFFFFFFFFF:FFFFFF::FF:FFFFFFF,FFFFNM:i:1MD:Z:4C146MC:Z:125M26SAS:i:146XS:i:70Yt:Z:NR 0 60 ATTCTCTTCTTGCCTGCATGGTTTCTGCTGAGTAGTCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGG +A00126:224:H7HMWDSX3:3:1110:19407:28902 ! 0 Test_seq 167163 - + NR A00126:224:H7HMWDSX3:3:1110:19407:28902145Test_seq16741019126M25S=167163-373AGGTGTTTGATTTTTAGGAGCTCCCAGTTATCGGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATACCTTGTATTAGGGCTCCTAGGGTTGTCCCAATTTTTTCTTCCATGATCGATCATTCGTCTTTTTATTGTTGAGFFF:FFF:FFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFNM:i:6MD:Z:32T44G2C22A0T2T18MC:Z:151MAS:i:96XS:i:86XA:Z:Test_seq,+213499,25S126M,8;Yt:Z:NR A00126:224:H7HMWDSX3:3:1110:19407:2890297Test_seq16716352151M=167410373AATGGCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAATCATGTAGATTTTAFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFF:FFFFFFFFF:FFFFF::FFFFFFFFFFFFFFFFFFF::FF,FFF::F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:80T70MC:Z:126M25SAS:i:146XS:i:121XA:Z:Test_seq,-213721,151M,6;Yt:Z:NR 0 52 AATGGCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAATCATGTAGATTTTA +A00126:224:H7HMWDSX3:3:1110:19180:29136 Test_seq 32280 Test_seq 32495 + - UU A00126:224:H7HMWDSX3:3:1110:19180:2913697Test_seq322806020S131M=32345216GGAGAAGTTCATTTTAAGAGTAAAATGCCTTTCATTTTTCAGTGCAATTAGAAAATCCTCTTGGAAGCTCAAAGTTTATACAGAGTTTTTTAAAATATACTCTTGATTGTATTTTAGCATTCTTTATTTATTTATTTTGTATTTTATATAAFFFFFFFFFFF,:FF,F,F:FFFFFF,:F,F:,F,FFFF,F,:FFFF,F:,FFFF,,,,,:F:F:F,F,,FFF,:FF,FFF,,::F:FFFF,FFF,:,,:,,:F,:FFFFFF,F,:,:FFF,:FFF:FFFFFF:FFFF,:,FFFF,,FFFFNM:i:7MD:Z:57C3T14C1C13G1G31G4MC:Z:151MAS:i:96XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1110:19180:29136145Test_seq3234560151M=32280-216TTTTTTAAAATCTCCTCTTGATTGTATGTGAGCATTCTTTATTTATTTATTTTGTATTTTAGATAAAGGTTTACAGAGGAAATTAGTATGACAGGATCTAAAAGTGCTTAATGTTCTTGAAGTAACATATTTAATGGGTGTCTGCTTCTCAFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:20S131MAS:i:151XS:i:23Yt:Z:UU 60 60 GGAGAAGTTCATTTTAAGAGTAAAATGCCTTTCATTTTTCAGTGCAATTAGAAAATCCTCTTGGAAGCTCAAAGTTTATACAGAGTTTTTTAAAATATACTCTTGATTGTATTTTAGCATTCTTTATTTATTTATTTTGTATTTTATATAA TTTTTTAAAATCTCCTCTTGATTGTATGTGAGCATTCTTTATTTATTTATTTTGTATTTTAGATAAAGGTTTACAGAGGAAATTAGTATGACAGGATCTAAAAGTGCTTAATGTTCTTGAAGTAACATATTTAATGGGTGTCTGCTTCTCA +A00126:224:H7HMWDSX3:3:1110:10628:29105 Test_seq 212528 Test_seq 212863 + - UU A00126:224:H7HMWDSX3:3:1110:10628:2910597Test_seq2125287151M=212713336ATTTCCCTTGAGAACGGGAACCAGACAAGGATGCTCTTTAACACCGCTCTTATTCAACATTATGCTAGAAGTCCTAGCCAGAGCAATTAGGCTAGACAAAGAAATAAAGGTCATCCGGATTGGCAAAGAGGAAGTAAAATTATCTCTATTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFNM:i:14MD:Z:0C4G4G8G5A8C5T3T11G4G7G40G4T10G24MC:Z:151MAS:i:85XS:i:78XA:Z:Test_seq,-168373,13S138M,12;Test_seq,+40206,39M3I109M,16;Yt:Z:UU A00126:224:H7HMWDSX3:3:1110:10628:29105145Test_seq21271331151M=212528-336AGGAATCCTCCAGAAAACTACTGAAACTAATAGAAGAGTTTGGCAGAGTCTCAGGTTATAAGATAAACATACAAAAATCACTTGGATTCCTCTACATCAACAAAAAGAACATCGAAGAGGAAATAACCAAATCAATACCATTCACAGTAGCFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:113A37MC:Z:151MAS:i:146XS:i:131XA:Z:Test_seq,+168175,151M,4;Yt:Z:UU 7 31 ATTTCCCTTGAGAACGGGAACCAGACAAGGATGCTCTTTAACACCGCTCTTATTCAACATTATGCTAGAAGTCCTAGCCAGAGCAATTAGGCTAGACAAAGAAATAAAGGTCATCCGGATTGGCAAAGAGGAAGTAAAATTATCTCTATTT AGGAATCCTCCAGAAAACTACTGAAACTAATAGAAGAGTTTGGCAGAGTCTCAGGTTATAAGATAAACATACAAAAATCACTTGGATTCCTCTACATCAACAAAAAGAACATCGAAGAGGAAATAACCAAATCAATACCATTCACAGTAGC +A00126:224:H7HMWDSX3:3:1110:18475:29481 Test_seq 166060 Test_seq 166553 + - UU A00126:224:H7HMWDSX3:3:1110:18475:29481161Test_seq16606060151M=166403494GTATCCCATTGGTCTCCTGCTCCCTCAGGAGTTGTCTGTTGTGCTCCCTGACAGGGCAGACATCAATTGTGGCCGGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCTFFFFFFFFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FF:FFFF:,FFNM:i:1MD:Z:64G86MC:Z:151MAS:i:146XS:i:85Yt:Z:UU A00126:224:H7HMWDSX3:3:1110:18475:2948181Test_seq16640360151M=166060-494CAGACCCCAGCCCCTGCTCCGCTGACCTTTGAAGCTTTCATTTTATCCCAGAAACCTCTTTGCTTTTAATCCAGTCCAATTAGGCTGACCTTCCTTGTATTGAGTGTTGTCTTTCCCTTCACCCAAAGCAGTTCTTATCTACAGATTGATCFFFFFFFFFFF:FFFFF,FFFFFFFFFFFFFFFFFFFFFFF,FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:4MD:Z:49G18G33T39T8MC:Z:151MAS:i:131XS:i:40Yt:Z:UU 60 60 GTATCCCATTGGTCTCCTGCTCCCTCAGGAGTTGTCTGTTGTGCTCCCTGACAGGGCAGACATCAATTGTGGCCGGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCT CAGACCCCAGCCCCTGCTCCGCTGACCTTTGAAGCTTTCATTTTATCCCAGAAACCTCTTTGCTTTTAATCCAGTCCAATTAGGCTGACCTTCCTTGTATTGAGTGTTGTCTTTCCCTTCACCCAAAGCAGTTCTTATCTACAGATTGATC +A00126:224:H7HMWDSX3:3:1110:23366:30655 ! 0 Test_seq 168631 - - MU A00126:224:H7HMWDSX3:3:1110:23366:3065597Test_seq1681630151M=168481469ATCTTCTTGGGGGCTACTGTGAATGGTATTGATTTGGTTATTTCCTCTTCGGTGTTCTTTTTGTTGATGTAGAGGAATCCAAGTGATTTTTGTATGTTTATTTTATAACTTGAGACTCTGCCAAACTGTTCTATTAGTTTCAGTAGTTTTCFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFF:FF:FFFFFFFNM:i:5MD:Z:6C101T0C9T7C23MC:Z:151MAS:i:126XS:i:126XA:Z:Test_seq,-212725,151M,5;Yt:Z:MU A00126:224:H7HMWDSX3:3:1110:23366:30655145Test_seq16848160151M=168163-469CCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGT:::FF:FFFFFFFFFFFFFFFFFF,FFFFF:FFFFFFFFFF:FFFF:FFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFNM:i:1MD:Z:14T136MC:Z:151MAS:i:146XS:i:91Yt:Z:MU 0 60 ATCTTCTTGGGGGCTACTGTGAATGGTATTGATTTGGTTATTTCCTCTTCGGTGTTCTTTTTGTTGATGTAGAGGAATCCAAGTGATTTTTGTATGTTTATTTTATAACTTGAGACTCTGCCAAACTGTTCTATTAGTTTCAGTAGTTTTC CCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGT +A00126:224:H7HMWDSX3:3:1110:1678:32330 Test_seq 163369 Test_seq 163747 + - UU A00126:224:H7HMWDSX3:3:1110:1678:3233097Test_seq16336960151M=163597379AGCCAGAAGCATAGCATCTACAGTCTTTCTGACTCTGTGTCCATTAATCACAGCTGCTTCTCTAACTCCGACCTCTGATAAGGACCCTTGGGATAATCTCTTCATCTCAAAATCCTTATTTCAAACACATCTGCAAAGCCCCTTTTGCCATFFFFFFFFFFF,:FFFFFFF:FFFFF:F:FFFFFFFFFFF,,:FFFFFFFFFFFFFFFFFFFFFF:F::FF,FFFF:F:FF:F,F,FF:F,FFFF,F:F:F:FFFFF::FFFF:FFF:FF:FFF,FFFF:,F,:FFFF:FFFFF,F::FFFNM:i:1MD:Z:124C26MC:Z:151MAS:i:146XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1110:1678:32330145Test_seq16359760151M=163369-379AGTGTATCATAGAAAATACATTGATGCCAGCACTGGTTTTAGAGCAGACCTGAGTTCAAATCCTGCCTTCAGCACCTAGTAGTTGTGTGACCATCGTGCCTTTGGGTTTCTGTTTTTTCATCCATAAAATAAGACTAAAATCCCAAGATTAFF:,FF,:FFFFFFFFFFFFF:FF:::FFF:FFFFF,FFFF,FF:FFFF::F:,::,FFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFF,FFFFFFF,F::FFFFFFFF:FF:FFFFFF:FFF:FF:FFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:40Yt:Z:UU 60 60 AGCCAGAAGCATAGCATCTACAGTCTTTCTGACTCTGTGTCCATTAATCACAGCTGCTTCTCTAACTCCGACCTCTGATAAGGACCCTTGGGATAATCTCTTCATCTCAAAATCCTTATTTCAAACACATCTGCAAAGCCCCTTTTGCCAT AGTGTATCATAGAAAATACATTGATGCCAGCACTGGTTTTAGAGCAGACCTGAGTTCAAATCCTGCCTTCAGCACCTAGTAGTTGTGTGACCATCGTGCCTTTGGGTTTCTGTTTTTTCATCCATAAAATAAGACTAAAATCCCAAGATTA +A00126:224:H7HMWDSX3:3:1110:23809:32706 Test_seq 150778 Test_seq 151184 + - UU A00126:224:H7HMWDSX3:3:1110:23809:3270697Test_seq15077860151M=151034407GTTGTCTTTCCCTTCACCTAAACTAGTTCTTAACTACTATCTAATTAGTGAATAGCCCCGCCCACGGTCCCGTCCCTCCCCCACTGGTAACCCCAGAAGAAAGTTTTCTTCTCAGTTTAAAATAATTATAAAGTTCTTATAATAGTGGTCTFFFFFFFFFFFF,FFFFFFFFFFF:FFFFFFFFFFFFF:FFFFF:FFFFF::::,:,FF,:FFFF,,,,,,,,FFF,:FFFF,F,FF,FFFF,F:,:F,,F,,:,,::,:,,,F,,,FFF::,F,,F::,:F,:F,F,::F:FF,:,:::FNM:i:11MD:Z:54C4T6T4T10T9A8T19C2T2C1C21MC:Z:151MAS:i:96XS:i:30Yt:Z:UU A00126:224:H7HMWDSX3:3:1110:23809:32706145Test_seq15103460151M=150778-407CGATGTGTAGTATTCCATTCTGTGAATATGCCATAATTTATTTATCCATTCATCTGTTGATGGGCATCTTGGTTGCTTCCAGCTTCTTGCTGTTATAAACAGTGCTGCAATAAACATGGGTGTGCATATATCTGTTCATGTAAAGGCTCCTFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:69Yt:Z:UU 60 60 GTTGTCTTTCCCTTCACCTAAACTAGTTCTTAACTACTATCTAATTAGTGAATAGCCCCGCCCACGGTCCCGTCCCTCCCCCACTGGTAACCCCAGAAGAAAGTTTTCTTCTCAGTTTAAAATAATTATAAAGTTCTTATAATAGTGGTCT CGATGTGTAGTATTCCATTCTGTGAATATGCCATAATTTATTTATCCATTCATCTGTTGATGGGCATCTTGGTTGCTTCCAGCTTCTTGCTGTTATAAACAGTGCTGCAATAAACATGGGTGTGCATATATCTGTTCATGTAAAGGCTCCT +A00126:224:H7HMWDSX3:3:1110:15799:33113 Test_seq 173420 Test_seq 173759 + - UU A00126:224:H7HMWDSX3:3:1110:15799:3311397Test_seq17342060151M=173609340ATTCTGCCCAGCTCTGCCTTACTCTCAGTCCCACTGCTTTTCCCATAAGGCCATGGAATCAGGCATGATGTGTAGAAGCATAAACTGGGGGGTCACTGAGTGAAGCAATTCTGACTAACACAGTCAGAGGTTCTCCACAGCCTTGAACGTAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFF,FFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF,FFF,FFFFFFFFFFFF,FFFF:FFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1110:15799:33113145Test_seq17360960151M=173420-340CCAGCGTTGGATTAGACTGGACCTTCTTTGAGGAGATGTCAAGGAAGGACTGTTCAGGAGCCAAGGGAAGTGTTCTTCATGGTGGCAATAACTGAAGTCTCCACTTAGGTCCTCGATATTTCCTAAGCTTGTTGTCATTAGTTGCTCTAAAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU 60 60 ATTCTGCCCAGCTCTGCCTTACTCTCAGTCCCACTGCTTTTCCCATAAGGCCATGGAATCAGGCATGATGTGTAGAAGCATAAACTGGGGGGTCACTGAGTGAAGCAATTCTGACTAACACAGTCAGAGGTTCTCCACAGCCTTGAACGTA CCAGCGTTGGATTAGACTGGACCTTCTTTGAGGAGATGTCAAGGAAGGACTGTTCAGGAGCCAAGGGAAGTGTTCTTCATGGTGGCAATAACTGAAGTCTCCACTTAGGTCCTCGATATTTCCTAAGCTTGTTGTCATTAGTTGCTCTAAA +A00126:224:H7HMWDSX3:3:1110:3812:33990 Test_seq 169218 Test_seq 169509 + - UU A00126:224:H7HMWDSX3:3:1110:3812:33990161Test_seq16921860151M=169359292GTAATCTGAAATGATTCTTTTAATTTCATTTGGTTCTGTTGTGATGTGGTCCTTCTCGTTTCTTATTTGGGTTATTTGTTTCCTTTCCTGTATTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGF:FFFFF:FF:F:F:FFF:F:FF::F:FF:FF:F,FFF:FFFFF:FFFF:F:FFFFFF:FFF:,FF,FFFFFFFF,FFFFF,F,FFFF:FFFFF:FFF:F:F:FFFFFFF,FFF:F:FFFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFNM:i:4MD:Z:33C16T16C23T59MC:Z:151MAS:i:131XS:i:72Yt:Z:UU A00126:224:H7HMWDSX3:3:1110:3812:3399081Test_seq16935960151M=169218-292AAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGGTTCTTTTGTTGCTCACTTTCTATTTGTTCAAGTTGTCGGGAC,FFFFFFFF,FFFFFFF,FFFFFFFFF:FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:146A4MC:Z:151MAS:i:146XS:i:66Yt:Z:UU 60 60 GTAATCTGAAATGATTCTTTTAATTTCATTTGGTTCTGTTGTGATGTGGTCCTTCTCGTTTCTTATTTGGGTTATTTGTTTCCTTTCCTGTATTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAG AAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGGTTCTTTTGTTGCTCACTTTCTATTTGTTCAAGTTGTCGGGAC +A00126:224:H7HMWDSX3:3:1110:9489:34428 Test_seq 170166 Test_seq 170469 + - UU A00126:224:H7HMWDSX3:3:1110:9489:34428161Test_seq17016660151M=170319304TATATAGTGTCCTTCTTTATCCTTTGTGGTGGATTTAAGTCTAAAGTCTTTTTTGTCAGAAATTAATATTGCTACTCCTCTTCTTTTTTGCTTATTGTTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFF:FFFFFFFFFF:FFFFFFF:FFFFFFF:FFFFFFFFF,FFFFFF:FFFFFFFFFFF:FFFFNM:i:4MD:Z:29C0A18A46A54MC:Z:151MAS:i:131XS:i:75Yt:Z:UU A00126:224:H7HMWDSX3:3:1110:9489:3442881Test_seq17031960151M=170166-304TCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCTGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFNM:i:1MD:Z:57C93MC:Z:151MAS:i:146XS:i:64Yt:Z:UU 60 60 TATATAGTGTCCTTCTTTATCCTTTGTGGTGGATTTAAGTCTAAAGTCTTTTTTGTCAGAAATTAATATTGCTACTCCTCTTCTTTTTTGCTTATTGTTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTA TCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCTGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATTTTGATGCCT +A00126:224:H7HMWDSX3:3:1111:24768:1360 ! 0 Test_seq 168456 - + NR A00126:224:H7HMWDSX3:3:1111:24768:1360145Test_seq16854959127M24S=168456-220TGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTGAGAGTTTGTATCATAAATGGGTGTTGGACTTTGTCAAATGCCTTTTCTGCATCAATTGATAAGATCCTCTCCTTTCTCTGTCGCTCTAGTFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:4MD:Z:60A7T18A26T12MC:Z:151MAS:i:107XS:i:78Yt:Z:NR A00126:224:H7HMWDSX3:3:1111:24768:136097Test_seq16845660151M=168549220GAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:39T111MC:Z:127M24SAS:i:146XS:i:91Yt:Z:NR 0 60 GAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTG +A00126:224:H7HMWDSX3:3:1111:29586:1720 ! 0 Test_seq 168674 - - NR A00126:224:H7HMWDSX3:3:1111:29586:172097Test_seq1682776026S125M=168524398AACATAGTAATATTTTACATATTTTAACTCTTCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGGTTTTCCATGTATACGATCATGTCATCTGCAAATAGTGATAGCTCTACTTCCTCCTTGCCAATCTGGATACCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFNM:i:3MD:Z:69G18A8T27MC:Z:151MAS:i:110XS:i:72Yt:Z:NR A00126:224:H7HMWDSX3:3:1111:29586:1720145Test_seq16852460151M=168277-398CTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATFF,FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:26S125MAS:i:151XS:i:0Yt:Z:NR 0 60 CTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGAT +A00126:224:H7HMWDSX3:3:1111:19352:2284 Test_seq 167332 Test_seq 213219 + + UU A00126:224:H7HMWDSX3:3:1111:19352:2284129Test_seq16733221151M=21321945888GAGATGTCATAGCTGAATATTCTTTCCCAGTCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTFFFFFFFFFFFFFFFFFFFFFF:FF,,:FFFFFFFFF,:FFFFFF:FFFFFFF:FFF:FFFF,F,FFFF,FFFFF,FFFF,F:FF:F,F:FFF:F:FF:FFFFFFFFFFFF:FFFFFF:FFFF:FFFFFFFFFF:FFFF,:FFFFFFFFFFNM:i:1MD:Z:36A114MC:Z:151MAS:i:146XS:i:136XA:Z:Test_seq,-213552,151M,3;Yt:Z:UU A00126:224:H7HMWDSX3:3:1111:19352:228465Test_seq2132191151M=167332-45888GCTACAGTAGTCAAAACAGCCTGGTACTGGTACAACAACAGGCACATAGACCAATGGAACAGAATTGAGAACCCAGATATAAATCCATCCATGTATGAGCAGCTGATATTTGACAAAGGACCAGTGTCGGTCAATTGGGGAAATGATAGTCFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:F:FFFFFFFF:FFF:FFFFFFFFFNM:i:2MD:Z:93A34A22MC:Z:151MAS:i:141XS:i:139XA:Z:Test_seq,-167669,151M,3;Yt:Z:UU 21 1 GAGATGTCATAGCTGAATATTCTTTCCCAGTCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGT GCTACAGTAGTCAAAACAGCCTGGTACTGGTACAACAACAGGCACATAGACCAATGGAACAGAATTGAGAACCCAGATATAAATCCATCCATGTATGAGCAGCTGATATTTGACAAAGGACCAGTGTCGGTCAATTGGGGAAATGATAGTC +A00126:224:H7HMWDSX3:3:1111:26467:7968 ! 0 Test_seq 168497 - + NR A00126:224:H7HMWDSX3:3:1111:26467:796881Test_seq16868360128M23S=168497-314TTTTATCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGTATTATTTTTTTGATGTGTTGTTGGATTAGTCCATCTTAGAACATGGAAGAFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:2MD:Z:33T66A27MC:Z:151MAS:i:118XS:i:55Yt:Z:NR A00126:224:H7HMWDSX3:3:1111:26467:7968161Test_seq16849760151M=168683314TTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF,:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF,:F:FFF:FFFFFFFFFFFFFFF:FFFFFFFFF:,FFFFFFFFFFNM:i:0MD:Z:151MC:Z:128M23SAS:i:151XS:i:96Yt:Z:NR 0 60 TTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAA +A00126:224:H7HMWDSX3:3:1111:29125:8124 ! 0 Test_seq 391538 - + NR A00126:224:H7HMWDSX3:3:1111:29125:8124145Test_seq39180360126M25S=391538-391AGCTGTACACTTGAGAGATAAACAGTTAAAAAGTGCCTTATGTGCCTTCCTGGTGTTTTCTGTGCACATATGGCCATATATTATGGGCATACCAAATCAAAAAAACAAGTTGCTGTCCAGGTGATCGATCCCTGTGCTGACTTTGGAATTC:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF,FFFFFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:126MC:Z:151MAS:i:126XS:i:0Yt:Z:NR A00126:224:H7HMWDSX3:3:1111:29125:812497Test_seq39153860151M=391803391GGTGCTCCTTGGAAACCCTGTGGGGCAGTTCTACTCTGTCCCATAGGGTCTCTATGAGTCGGAATTGTCTCAAGGGCATCGGGTATGTATGTATATATGTAGAAATGACAGATTTTGCCATGTTAGTTTTCCACAATGTTTTCGGTGAAGTFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:126M25SAS:i:151XS:i:31Yt:Z:NR 0 60 GGTGCTCCTTGGAAACCCTGTGGGGCAGTTCTACTCTGTCCCATAGGGTCTCTATGAGTCGGAATTGTCTCAAGGGCATCGGGTATGTATGTATATATGTAGAAATGACAGATTTTGCCATGTTAGTTTTCCACAATGTTTTCGGTGAAGT +A00126:224:H7HMWDSX3:3:1111:2871:8187 ! 0 Test_seq 168666 - - NR A00126:224:H7HMWDSX3:3:1111:2871:8187161Test_seq1683503827S124M=168516317TGTGAGTTCTACCAAACATTTAGATCTTGTCATCTGCAAATAGTGATAACTTTACTTCCTCCTTGCCAATCCAGATACCCTTTATTTCTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGCAGTGATAAF:F,F:FFFF:FFFF:FFFFFFF:FFFFFFFFFFFFFFF:FF,FFFFFF:FFFF:F::F:,FFF:,,FFF:F:FFFFF:FFFFFFFF,FFFF:FFF,::FFFFFF,,:FF,FFF:FFFFF,F,,FFFFFFFFFFFFFFFFF:FFFFFFFFFNM:i:5MD:Z:15A5G22T0G70G7MC:Z:151MAS:i:99XS:i:80XA:Z:Test_seq,-212565,130M21S,10;Yt:Z:NR A00126:224:H7HMWDSX3:3:1111:2871:818781Test_seq16851660151M=168350-317CAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCAATTFF::F:FFFFFFFFFFFFFFF:FFFFF:FFFFFFF,FFF:,:FFFFFF:,FF,FFFFFFF,FF:F:,FF,FFFFFFFFFFFFFFFF::FF,,FFF:FF,,FFFFF:F:FFFF:FFF,,FFFFF:FF:FFFFFFFFFFFFF,FFFF,FFFFFNM:i:1MD:Z:147T3MC:Z:27S124MAS:i:147XS:i:67Yt:Z:NR 0 60 CAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCAATT +A00126:224:H7HMWDSX3:3:1111:25301:8672 ! 0 Test_seq 170261 - + NR A00126:224:H7HMWDSX3:3:1111:25301:867281Test_seq17043060120M31S=170261-289TATAGATAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTTTTCATGTGAGTTGTTGGCCATTTCATTTTTCCACATACTTTTTTGTGCCGAGACGTTTTTCTTAGTAGATTGTGAGATCAGTCCTACCATTTTACAGTCTTGCCAGCAATFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFNM:i:3MD:Z:49T10T28T30MC:Z:151MAS:i:105XS:i:22Yt:Z:NR A00126:224:H7HMWDSX3:3:1111:25301:8672161Test_seq17026160151M=170430289TATTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGGTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,NM:i:1MD:Z:44T106MC:Z:120M31SAS:i:146XS:i:70Yt:Z:NR 0 60 TATTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGGTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCC +A00126:224:H7HMWDSX3:3:1111:11153:8719 Test_seq 170147 Test_seq 170406 + - UU A00126:224:H7HMWDSX3:3:1111:11153:871997Test_seq17014760151M=170256260CAATTGTCCCTTTTATCATTATATAGTGTCCTTCTTTATCCTTTGTGGCGGATTTAAGTCTAAAGTCTATTTTGTCAGAAATTAGTATTGCTACTCCTCTTCTTTTTTGCTTATTATTTGCTTAATATATTTTTTTACATCCTTTGAGTTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F::FFFF:FFFFFFFFF:FFFFFFFFFFFFFF:FFFFFFFFFFFF,FF:F:FFF:FFFFFNM:i:4MD:Z:49A34A38G12C14MC:Z:151MAS:i:131XS:i:55Yt:Z:UU A00126:224:H7HMWDSX3:3:1111:11153:8719145Test_seq17025660151M=170147-260CTTATTATTTGCTTAATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCGTGACTCTCTGTCTCTTTATTGGTGCATTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:14G136MC:Z:151MAS:i:146XS:i:73Yt:Z:UU 60 60 CAATTGTCCCTTTTATCATTATATAGTGTCCTTCTTTATCCTTTGTGGCGGATTTAAGTCTAAAGTCTATTTTGTCAGAAATTAGTATTGCTACTCCTCTTCTTTTTTGCTTATTATTTGCTTAATATATTTTTTTACATCCTTTGAGTTT CTTATTATTTGCTTAATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCGTGACTCTCTGTCTCTTTATTGGTGCATTT +A00126:224:H7HMWDSX3:3:1111:21468:9079 Test_seq 117403 Test_seq 117847 + - UU A00126:224:H7HMWDSX3:3:1111:21468:9079161Test_seq11740360151M=117697445AATCCACTTAAGTATTTGAATGTTATTTCAACCTCTTTCTATGAGGAAGAGCTTTGGGAAAAAATAAGTAGAAAGTGAAAATATCGATAGTGATTAATGAGTGATGGCTGGCTTTGACTGCTGTAGGAGTAGGTAGGACAACCGCTACAAAFFFFFFFFFFFFFF,F:FFFFF:FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF,FFFFFFFFF:FFF:FFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFF:NM:i:1MD:Z:148G2MC:Z:151MAS:i:148XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1111:21468:907981Test_seq11769760151M=117403-445TCCTCTTTCTGTGGGATGGACAAGGATGGAGCCTAGGGCCCCATCCCTGTCACATTTCTGCACAGTCCTCAGACACTTGCAGAAAGTGTTGCCGGTTTTTAGCACATCTTTCTCTGGAGATGAAACTGGCTCAGCACCAACCAGAGGGCACFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU 60 60 AATCCACTTAAGTATTTGAATGTTATTTCAACCTCTTTCTATGAGGAAGAGCTTTGGGAAAAAATAAGTAGAAAGTGAAAATATCGATAGTGATTAATGAGTGATGGCTGGCTTTGACTGCTGTAGGAGTAGGTAGGACAACCGCTACAAA TCCTCTTTCTGTGGGATGGACAAGGATGGAGCCTAGGGCCCCATCCCTGTCACATTTCTGCACAGTCCTCAGACACTTGCAGAAAGTGTTGCCGGTTTTTAGCACATCTTTCTCTGGAGATGAAACTGGCTCAGCACCAACCAGAGGGCAC +A00126:224:H7HMWDSX3:3:1111:3884:9909 Test_seq 196478 Test_seq 319237 + - UR A00126:224:H7HMWDSX3:3:1111:3884:990997Test_seq1964786032M119S=319087122760TCCTCATTCATAAATAGGGCTTGTTATAGATCACTCACGGCAACCCCGTGTGTGCAGAGTAGAGCTGCACTCCACAGTGGTTTTCAAGGCTGTGAACTTTCAGAAACAGGTCACTAGTCCTTTCTTTCAAGGTGCCTCTGAGTGGGTTCAAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF,FFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFF,FFFFFFFFFFNM:i:0MD:Z:32MC:Z:151MAS:i:32XS:i:0SA:Z:Test_seq,318867,+,32S119M,60,0;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1111:3884:99092145Test_seq3188676032H119M=319087371ACTCACGGCAACCCCGTGTGTGCAGAGTAGAGCTGCACTCCACAGTGGTTTTCAAGGCTGTGAACTTTCAGAAACAGGTCACTAGTCCTTTCTTTCAAGGTGCCTCTGAGTGGGTTCAAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF,FFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFF,FFFFFFFFFFNM:i:0MD:Z:119MC:Z:151MAS:i:119XS:i:0SA:Z:Test_seq,196478,+,32M119S,60,0;Yt:Z:UR A00126:224:H7HMWDSX3:3:1111:3884:9909145Test_seq31908760151M=196478-122760GGTGATGGTTTAGTGGGGTGGATGGGTAGAGTAGTGAGAGGCATCAGCTGCTCTCCTGTTGAAGCTGGAAGACATCATCAAGATCAGACAGCCTGAGAGAAGCGTCTTGGGAGGAAGTGGGTACGAGCATTGGCTGTCTAAGAGTTGAGATFFFFFFFFFFFFFFFFFF,F:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:32M119SAS:i:151XS:i:0Yt:Z:UR 60 60 TCCTCATTCATAAATAGGGCTTGTTATAGATCACTCACGGCAACCCCGTGTGTGCAGAGTAGAGCTGCACTCCACAGTGGTTTTCAAGGCTGTGAACTTTCAGAAACAGGTCACTAGTCCTTTCTTTCAAGGTGCCTCTGAGTGGGTTCAA GGTGATGGTTTAGTGGGGTGGATGGGTAGAGTAGTGAGAGGCATCAGCTGCTCTCCTGTTGAAGCTGGAAGACATCATCAAGATCAGACAGCCTGAGAGAAGCGTCTTGGGAGGAAGTGGGTACGAGCATTGGCTGTCTAAGAGTTGAGAT +A00126:224:H7HMWDSX3:3:1111:27254:10113 ! 0 Test_seq 169321 - + NR A00126:224:H7HMWDSX3:3:1111:27254:10113145Test_seq16962860119M32S=169321-426CGTTGCTTTCTATGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTATTGTTCATTTTCCAAGTATTTGATTTCTTTTCCCTCGTTTTTCTGTTATTGATCGATCTCTTTATTTGCACTGCTTTAGGFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFFFNM:i:4MD:Z:0T11A22G70A12MC:Z:151MAS:i:103XS:i:0Yt:Z:NR A00126:224:H7HMWDSX3:3:1111:27254:1011397Test_seq16932160151M=169628426AGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGATTCTFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFF,:FFFF:F,FFFF,:FF:FFFF:FFFFFFFFFF:FFFFFFFFFFFFNM:i:1MD:Z:146G4MC:Z:119M32SAS:i:146XS:i:112Yt:Z:NR 0 60 AGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGATTCT +A00126:224:H7HMWDSX3:3:1111:22200:10222 Test_seq 117403 Test_seq 117847 + - UU A00126:224:H7HMWDSX3:3:1111:22200:10222161Test_seq11740360151M=117697445AATCCACTTAAGTATTTGAATGTTATTTCAACCTCTTTCTATGAGGAAGAGCTTTGGGAAAAAATAAGTAGAAAGTGAAAATATCGATAGTGATTAATGAGTGATGGCTGGCTTTGACTGCTGTAGGAGTAGGTAGGACAACCGCTACAAAFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFF,FF:FFFF,FFFFFFFFFFFFFFF,F:FFFFFFFFFFFF:FFFFF:FFFFFFFFFFF,FF:FFFFFFFF:F:FFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:148G2MC:Z:151MAS:i:148XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1111:22200:1022281Test_seq11769760151M=117403-445TCCTCTTTCTGTGGGATGGACAAGGATGGAGCCTAGGGCCCCATCCCTGTCACATTTCTGCACAGTCCTCAGACACTTGCAGAAAGTGTTGCCGGTTTTTAGCACATCTTTCTCTGGAGATGAAACTGGCTCAGCACCAACCAGAGGGCACFFFFFF,FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFF::FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU 60 60 AATCCACTTAAGTATTTGAATGTTATTTCAACCTCTTTCTATGAGGAAGAGCTTTGGGAAAAAATAAGTAGAAAGTGAAAATATCGATAGTGATTAATGAGTGATGGCTGGCTTTGACTGCTGTAGGAGTAGGTAGGACAACCGCTACAAA TCCTCTTTCTGTGGGATGGACAAGGATGGAGCCTAGGGCCCCATCCCTGTCACATTTCTGCACAGTCCTCAGACACTTGCAGAAAGTGTTGCCGGTTTTTAGCACATCTTTCTCTGGAGATGAAACTGGCTCAGCACCAACCAGAGGGCAC +A00126:224:H7HMWDSX3:3:1111:17499:11537 Test_seq 171298 Test_seq 171659 + - UU A00126:224:H7HMWDSX3:3:1111:17499:1153797Test_seq17129860151M=171509362TGGGGTTCGATGAGCATCTTGGATAGATATCCTTTCGTCTTTCATGATGTCAGGGAAGTTTTCTGTCAGGAGTTCTTCAACTATTTTCTCTGTGTTTTCTGTCCCCCCTCCCTGTTCTGGGACTCCAATCACTCGCAAGTTATCCTTCTTGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF:FFF:F:FFFFFF,FF:F,FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF:FFFF,F:FFFFFFFFFF:NM:i:3MD:Z:62G39G15T32MC:Z:151MAS:i:136XS:i:61Yt:Z:UU A00126:224:H7HMWDSX3:3:1111:17499:11537145Test_seq17150960151M=171298-362TTTTCAGCTATGTTGGAGTTGATTCCCTGGTCCTCCAGAAGTCCCAGTCTACATTCTAATTGCTCGAGTCTGCTCCTCTGACTTTCTATTGCGTTGTCTAATTCTGTAATTTTATTGTTAATCTTTTGGATTTCTACATGCTGTCTCTCTAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:74T76MC:Z:151MAS:i:146XS:i:47Yt:Z:UU 60 60 TGGGGTTCGATGAGCATCTTGGATAGATATCCTTTCGTCTTTCATGATGTCAGGGAAGTTTTCTGTCAGGAGTTCTTCAACTATTTTCTCTGTGTTTTCTGTCCCCCCTCCCTGTTCTGGGACTCCAATCACTCGCAAGTTATCCTTCTTG TTTTCAGCTATGTTGGAGTTGATTCCCTGGTCCTCCAGAAGTCCCAGTCTACATTCTAATTGCTCGAGTCTGCTCCTCTGACTTTCTATTGCGTTGTCTAATTCTGTAATTTTATTGTTAATCTTTTGGATTTCTACATGCTGTCTCTCTA +A00126:224:H7HMWDSX3:3:1111:19994:11788 Test_seq 20289 Test_seq 168429 - + UR A00126:224:H7HMWDSX3:3:1111:19994:117882193Test_seq16865760120M31H=168429-348TGCATCTATTGATAAGATGATGTGGTTTTTATCTTTTGTTTTATTTATGAGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFNM:i:3MD:Z:18C30T9T60MC:Z:151MAS:i:105XS:i:50SA:Z:Test_seq,20263,-,124S27M,7,1;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1111:19994:11788145Test_seq202637124S27M=168429148141TGCATCTATTGATAAGATGATGTGGTTTTTATCTTTTGTTTTATTTATGAGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCGATCTTTTCTTTCTTTTTAATGACCTCTTGCF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFNM:i:1MD:Z:6G20MC:Z:151MAS:i:22XS:i:20SA:Z:Test_seq,168657,-,120M31S,60,3;XA:Z:Test_seq,-55966,131S20M,0;Yt:Z:UR A00126:224:H7HMWDSX3:3:1111:19994:1178897Test_seq16842960151M=20263-148141CCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFF,FFFFFFFFFFFFFFFFF:FFFFFFFF,FFFFFFFFFFFFFFFF:FF,FFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFF,F,F:FFFFFF,NM:i:1MD:Z:66T84MC:Z:124S27MAS:i:146XS:i:78Yt:Z:UR 7 60 TGCATCTATTGATAAGATGATGTGGTTTTTATCTTTTGTTTTATTTATGAGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCGATCTTTTCTTTCTTTTTAATGACCTCTTGC CCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTG +A00126:224:H7HMWDSX3:3:1111:20690:11929 ! 0 Test_seq 167597 - - NR A00126:224:H7HMWDSX3:3:1111:20690:1192997Test_seq1672982124S50M1I76M=167447300TTTGAAGGCTTGTCTTGTGAATTTAATCATGTAGATTTTAGAGATCAGGCGCTGGTCTGAGATGTCATAGCTGACATATTCTTTCCCAGTCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF,FFFNM:i:3MD:Z:25A44A55MC:Z:151MAS:i:109XS:i:99XA:Z:Test_seq,-213611,74M1I52M24S,5;Test_seq,+400719,24S50M1I76M,7;Yt:Z:NR A00126:224:H7HMWDSX3:3:1111:20690:11929145Test_seq16744760151M=167298-300TCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATGCCCTGTATTAGGGCTCCTAGGGTTGATCCTATTTTTTCTTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:24S50M1I76MAS:i:151XS:i:101Yt:Z:NR 0 60 TCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATGCCCTGTATTAGGGCTCCTAGGGTTGATCCTATTTTTTCTTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGG +A00126:224:H7HMWDSX3:3:1111:24795:12649 Test_seq 168660 Test_seq 212799 - - UU A00126:224:H7HMWDSX3:3:1111:24795:12649113Test_seq16851060151M=21264944140TGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGGACTTTGTCAAATGCCTTTTCTGCAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:126A24MC:Z:151MAS:i:146XS:i:78Yt:Z:UU A00126:224:H7HMWDSX3:3:1111:24795:12649177Test_seq21264925151M=168510-44140GGCAAGGAAGAAGTAAAGTTATCACCATTTGCAGATGACATGATTATATACACAGAAAACCCTAAGGAATCCAACAGAAAACTACTGAAACTAATAGAAGAGTTTGGCAGAGTCTCAGGTTATAAAATAAACATACAAAAATCACTTGGATFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFNM:i:10MD:Z:8G8A5T1T18C0T17T8T0C51G25MC:Z:151MAS:i:101XS:i:86XA:Z:Test_seq,+168239,151M,13;Yt:Z:UU 60 25 TGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGGACTTTGTCAAATGCCTTTTCTGCA GGCAAGGAAGAAGTAAAGTTATCACCATTTGCAGATGACATGATTATATACACAGAAAACCCTAAGGAATCCAACAGAAAACTACTGAAACTAATAGAAGAGTTTGGCAGAGTCTCAGGTTATAAAATAAACATACAAAAATCACTTGGAT +A00126:224:H7HMWDSX3:3:1111:8802:14732 Test_seq 170230 Test_seq 170661 + - UU A00126:224:H7HMWDSX3:3:1111:8802:14732161Test_seq17023060151M=170511432AATATTGCTACTCCTCTTCTTTTTTGCTTATTATTTGCTTGATATATCTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCGTGAFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF,FFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFNM:i:1MD:Z:47T103MC:Z:151MAS:i:146XS:i:72Yt:Z:UU A00126:224:H7HMWDSX3:3:1111:8802:1473281Test_seq17051160151M=170230-432TTTTGTGCTGAGACGTTTTTCTTAGTAGATTGTGAGATCCTCATTTTCATAATGTTTAACTTTATGTTTGTTGAGTTGTTACGTTTTTCTTGAGTTATGGAATCGATATTCCTTTTTGTGGTTACCTTATTATTTACCCCTATTTTTCTAAFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:2MD:Z:76C26T47MC:Z:151MAS:i:141XS:i:37Yt:Z:UU 60 60 AATATTGCTACTCCTCTTCTTTTTTGCTTATTATTTGCTTGATATATCTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCGTGA TTTTGTGCTGAGACGTTTTTCTTAGTAGATTGTGAGATCCTCATTTTCATAATGTTTAACTTTATGTTTGTTGAGTTGTTACGTTTTTCTTGAGTTATGGAATCGATATTCCTTTTTGTGGTTACCTTATTATTTACCCCTATTTTTCTAA +A00126:224:H7HMWDSX3:3:1111:18756:15843 ! 0 Test_seq 226350 - + NR A00126:224:H7HMWDSX3:3:1111:18756:15843145Test_seq22664460123M28S=226350-417TGGGCCAGAGGCCAGTGCAGGACAGGCTCTGGTAGGCTATGCCTGTGTTGCTCGAAGGTGCAATGCTCAGCATATGGTGCAGGTAGGCAGGAAGGAGGGGGGAGGTTGTGATGTGTAGAGCTATAAGCACTTTCCATTGTTGTTGTTGTTGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:123MC:Z:151MAS:i:123XS:i:0Yt:Z:NR A00126:224:H7HMWDSX3:3:1111:18756:1584397Test_seq22635060151M=226644417GTTTCTTGAATACGTTTTGATATTGTCTGCTGTCCTTGGAACATTCAGTAGTTATTTTCTTCATTTATTGGCTGTAGATTTGTTTGTTTCATCCTGCTTTTTTGTTTTATTTGGTTATGTCTGAGCAGGCAGGCTGTGTGTTCTTTGTTGTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF,:FFF:FF:FFFFFFFFFFFFF:FFFFFFF:FFF,FF:FNM:i:1MD:Z:12T138MC:Z:123M28SAS:i:146XS:i:49Yt:Z:NR 0 60 GTTTCTTGAATACGTTTTGATATTGTCTGCTGTCCTTGGAACATTCAGTAGTTATTTTCTTCATTTATTGGCTGTAGATTTGTTTGTTTCATCCTGCTTTTTTGTTTTATTTGGTTATGTCTGAGCAGGCAGGCTGTGTGTTCTTTGTTGT +A00126:224:H7HMWDSX3:3:1111:23647:16172 Test_seq 165973 Test_seq 166326 + - UU A00126:224:H7HMWDSX3:3:1111:23647:1617297Test_seq16597360151M=166176354CAGAAGTTCTGGGGAGCATTGTCTCTGGGATTCCTCTAGTCGCATTCATATCATTAGGTATGGTCTTTTTATGAGAATTTGGGGTCTGTATCCCATTGGTCTCCTGCTCCCTCAGGAGTTGTCTGTTGTGCTCCCTAGCAGGGCAGACATCFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFNM:i:3MD:Z:41A94G0A13MC:Z:151MAS:i:136XS:i:42Yt:Z:UU A00126:224:H7HMWDSX3:3:1111:23647:16172145Test_seq16617660151M=165973-354CTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCTTAGTTGTCGTGTGACCTTGGTGTTCTTCCTTTGCCTTTGCTCCAGGTGGGTTGAGACCAATTGATGTATCTTAGATGGCCGCTTGTTGGCATTTAGGACCCCAGGTGCCACAATTCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:FF:FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:48G102MC:Z:151MAS:i:146XS:i:50Yt:Z:UU 60 60 CAGAAGTTCTGGGGAGCATTGTCTCTGGGATTCCTCTAGTCGCATTCATATCATTAGGTATGGTCTTTTTATGAGAATTTGGGGTCTGTATCCCATTGGTCTCCTGCTCCCTCAGGAGTTGTCTGTTGTGCTCCCTAGCAGGGCAGACATC CTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCTTAGTTGTCGTGTGACCTTGGTGTTCTTCCTTTGCCTTTGCTCCAGGTGGGTTGAGACCAATTGATGTATCTTAGATGGCCGCTTGTTGGCATTTAGGACCCCAGGTGCCACAATTC +A00126:224:H7HMWDSX3:3:1111:27841:16454 Test_seq 39072 Test_seq 169311 + + UU A00126:224:H7HMWDSX3:3:1111:27841:16454129Test_seq3907238151M=169311130240GAAATTCATAGAATGCAATGAGAATGAAAATACTTCCTATCAAAACGTTTGGGACACAGTGAAAGCAGGGCTCAGAGGTCAATTTATATCAATAAATGCACACATACATAAAGAAGAAAGAGCCAAAATCAGAGAACTGTCCCTATAACTTFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFNM:i:11MD:Z:14T3C4C6C15C11A9T15C0G45A13C5MC:Z:151MAS:i:96XS:i:72Yt:Z:UU A00126:224:H7HMWDSX3:3:1111:27841:1645465Test_seq16931160151M=39072-130240TTCTTTAGTCAGTCTGGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:::FF:F:FFFFF,FFFFFFFFFFFFFFFFFFFFF:FFFFFFNM:i:1MD:Z:15A135MC:Z:151MAS:i:146XS:i:116Yt:Z:UU 38 60 GAAATTCATAGAATGCAATGAGAATGAAAATACTTCCTATCAAAACGTTTGGGACACAGTGAAAGCAGGGCTCAGAGGTCAATTTATATCAATAAATGCACACATACATAAAGAAGAAAGAGCCAAAATCAGAGAACTGTCCCTATAACTT TTCTTTAGTCAGTCTGGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCT +A00126:224:H7HMWDSX3:3:1111:5692:17863 Test_seq 168773 Test_seq 169233 + - UU A00126:224:H7HMWDSX3:3:1111:5692:1786397Test_seq16877360151M=169099461GATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTCTATTGGCTAGAATTTTGTTGAGGATTTTTGCATCTATGTTCATGAGGGATATAGGTCTAAAATTTTCTTTTTTTGTAATGTCTTTACCTGGTTTTGGTATCAGGGAGATGGTFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:141A9MC:Z:135M16SAS:i:146XS:i:91Yt:Z:UU A00126:224:H7HMWDSX3:3:1111:5692:17863145Test_seq16909954135M16S=168773-461GTTATGCGTCTATTTAGTTGTTCTACTTCTGAATGTGTTAGTTTAGGTAGGTAGTGTTTTTCCAGGAATTCATCCATTTCTTCTAGGTTTTCAAATTTGTGAGAGTACAATTTTTCGTAGTAATCTGAAATGATTAATTGTGGAAAGTATAFFF:FFFFFFFFFFF:FFFF,FFFFFFFFFFFFFFFFF:FF:F:FFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:6MD:Z:6G55G1A35T1C11A20MC:Z:151MAS:i:105XS:i:76Yt:Z:UU 60 54 GATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTCTATTGGCTAGAATTTTGTTGAGGATTTTTGCATCTATGTTCATGAGGGATATAGGTCTAAAATTTTCTTTTTTTGTAATGTCTTTACCTGGTTTTGGTATCAGGGAGATGGT GTTATGCGTCTATTTAGTTGTTCTACTTCTGAATGTGTTAGTTTAGGTAGGTAGTGTTTTTCCAGGAATTCATCCATTTCTTCTAGGTTTTCAAATTTGTGAGAGTACAATTTTTCGTAGTAATCTGAAATGATTAATTGTGGAAAGTATA +A00126:224:H7HMWDSX3:3:1111:10402:18474 Test_seq 165665 Test_seq 166105 + - UU A00126:224:H7HMWDSX3:3:1111:10402:1847497Test_seq16566560151M=165955441TCAAGTGAACATTTACCATTCCAATCAGTCTGTCACATGTAGGTTTACATACATCATACTCCCTTCTCCCACTTGCTCTCCCCCTATTGAGTCAACCCTTTCAGTCTCTCGTTTCCTGCCAATTTTGCCATCTTCCCTCTCTCTCTATCTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFF,FFFFF:FFFFFFFF,FFFFFF:,FF::FFF:FFFFFFF:FFFF:NM:i:4MD:Z:80G13G20G19T15MC:Z:151MAS:i:131XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1111:10402:18474145Test_seq16595560151M=165665-441GTTCCTGTCCTGTGCCATCAGAAGTTCTGGGGAGCATTGTCTCTGGGATTCCTCTAGTCACATTCATATCATTAGGTATGGTCTTTTTATGAGAATTTGGGGTCTGTATCCCATTGGCCTCCTGCTCCCTCAGGAGTTGTCTGTTGTGCTCF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:117T33MC:Z:151MAS:i:146XS:i:35Yt:Z:UU 60 60 TCAAGTGAACATTTACCATTCCAATCAGTCTGTCACATGTAGGTTTACATACATCATACTCCCTTCTCCCACTTGCTCTCCCCCTATTGAGTCAACCCTTTCAGTCTCTCGTTTCCTGCCAATTTTGCCATCTTCCCTCTCTCTCTATCTT GTTCCTGTCCTGTGCCATCAGAAGTTCTGGGGAGCATTGTCTCTGGGATTCCTCTAGTCACATTCATATCATTAGGTATGGTCTTTTTATGAGAATTTGGGGTCTGTATCCCATTGGCCTCCTGCTCCCTCAGGAGTTGTCTGTTGTGCTC +A00126:224:H7HMWDSX3:3:1111:13024:18787 Test_seq 170380 Test_seq 170718 + - UU A00126:224:H7HMWDSX3:3:1111:13024:1878797Test_seq17038060151M=170580339ACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTTTTCATGTGTGTTGTTGGCCATTTCATTTTTCCACATACTTTTTTGTGCTGAGACGTTTTTFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFF,FFF,F::FFFF:FFF,FFF,FFF:FFFFNM:i:1MD:Z:110T40MC:Z:14M12I125MAS:i:146XS:i:48Yt:Z:UU A00126:224:H7HMWDSX3:3:1111:13024:18787145Test_seq1705806014M12I125M=170380-339GTTGAGTCGTTACGTTTTTCTTGGCTTTTTTCTTGAGTTATGGAGTTGATATTCCTTTTTGTGGTTACCTTATTATTTACCCCTATTTTTCTAAGTAAAAACCTAACTTGTATCATTCTATATCGCCTTGTATCACTCTCCATCTGGCAGTFFFFFFFFFFFF,F::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:16MD:Z:32A69G2G6A26MC:Z:151MAS:i:105XS:i:37Yt:Z:UU 60 60 ACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTTTTCATGTGTGTTGTTGGCCATTTCATTTTTCCACATACTTTTTTGTGCTGAGACGTTTTT GTTGAGTCGTTACGTTTTTCTTGGCTTTTTTCTTGAGTTATGGAGTTGATATTCCTTTTTGTGGTTACCTTATTATTTACCCCTATTTTTCTAAGTAAAAACCTAACTTGTATCATTCTATATCGCCTTGTATCACTCTCCATCTGGCAGT +A00126:224:H7HMWDSX3:3:1111:26612:18834 Test_seq 212573 Test_seq 213008 + - UU A00126:224:H7HMWDSX3:3:1111:26612:1883497Test_seq21257350151M=212858436GCTCTTATTCAACATTGTGCTAGAAGTCCTAGCCAGAGCAATTAGGCTAGACAAAGAAATAAAGGGCATCCGGATTGGCAAGGAGGAAGTAAAATTATCTCTATTTGCAGATGACATGATCTTATACACAGAAAACCCTAAGGAATCCTCCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFNM:i:4MD:Z:11G12G45T68T11MC:Z:151MAS:i:131XS:i:105XA:Z:Test_seq,+40248,151M,10;Yt:Z:UU A00126:224:H7HMWDSX3:3:1111:26612:18834145Test_seq21285860151M=212573-436AGTAGCCCCCAAGAAGATAAAATACTTAGGAATAAATCTTACCAAAGATGTAAAAGACCTATACAAAGAAAATTACAAAGTACTACCACAAGAAACTAAAAAGGACCTACTTAAGTGGAAAAACATACCTTGCTCATGGATAGGAAGACTTFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:86T64MC:Z:151MAS:i:146XS:i:85Yt:Z:UU 50 60 GCTCTTATTCAACATTGTGCTAGAAGTCCTAGCCAGAGCAATTAGGCTAGACAAAGAAATAAAGGGCATCCGGATTGGCAAGGAGGAAGTAAAATTATCTCTATTTGCAGATGACATGATCTTATACACAGAAAACCCTAAGGAATCCTCC AGTAGCCCCCAAGAAGATAAAATACTTAGGAATAAATCTTACCAAAGATGTAAAAGACCTATACAAAGAAAATTACAAAGTACTACCACAAGAAACTAAAAAGGACCTACTTAAGTGGAAAAACATACCTTGCTCATGGATAGGAAGACTT +A00126:224:H7HMWDSX3:3:1111:9417:19272 Test_seq 221904 Test_seq 306145 + - UR A00126:224:H7HMWDSX3:3:1111:9417:1927297Test_seq2219041221M130S=30599584242ATTTCCTTTGCCCATTTTTTAGATCAAAAGGAAGAGAGAGATATTTACAATGTCTTTTCTAATTTCTGAGTGTTTTTCTAAGTAGTCAAGTGACTGTTCACTTAGATTTAAGACATTTCTCTTCTCCTGTCATCAGCATTAGAGCCCAGATFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF,FFFFF,FFFFF:NM:i:0MD:Z:21MC:Z:151MAS:i:21XS:i:0SA:Z:Test_seq,305900,+,20S131M,60,0;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1111:9417:192722145Test_seq3059006020H131M=305995246AGATCAAAAGGAAGAGAGAGATATTTACAATGTCTTTTCTAATTTCTGAGTGTTTTTCTAAGTAGTCAAGTGACTGTTCACTTAGATTTAAGACATTTCTCTTCTCCTGTCATCAGCATTAGAGCCCAGATFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF,FFFFF,FFFFF:NM:i:0MD:Z:131MC:Z:151MAS:i:131XS:i:0SA:Z:Test_seq,221904,+,21M130S,12,0;Yt:Z:UR A00126:224:H7HMWDSX3:3:1111:9417:19272145Test_seq30599560151M=221904-84242TTTCTCTTCTCCTGTCATCAGCATTAGAGCCCAGATACTAAAGAGGCAAAAGTCATGGAAACCACTGGAGGGCCATTATCTTTCCTCATCTCCCCCATGTTGCCCCATCTCATCACAAAGGTCTGCGGTTGGTAAAGAGAGGAACTGGATGFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:21M130SAS:i:151XS:i:0Yt:Z:UR 12 60 ATTTCCTTTGCCCATTTTTTAGATCAAAAGGAAGAGAGAGATATTTACAATGTCTTTTCTAATTTCTGAGTGTTTTTCTAAGTAGTCAAGTGACTGTTCACTTAGATTTAAGACATTTCTCTTCTCCTGTCATCAGCATTAGAGCCCAGAT TTTCTCTTCTCCTGTCATCAGCATTAGAGCCCAGATACTAAAGAGGCAAAAGTCATGGAAACCACTGGAGGGCCATTATCTTTCCTCATCTCCCCCATGTTGCCCCATCTCATCACAAAGGTCTGCGGTTGGTAAAGAGAGGAACTGGATG +A00126:224:H7HMWDSX3:3:1111:17526:19288 Test_seq 152698 Test_seq 152983 + - UU A00126:224:H7HMWDSX3:3:1111:17526:1928897Test_seq15269860151M=152848286ATCCCCTTTATTTCTTTATCTAGCCTAATTGCTCTGGCTAGGACCTCCAACACAGTGTTGAATAAGAACGGTGATAAAGGGCATCCTTGTCTGGGTCCCAGTCTCAGTGGGAATGCTTTCAGGCTCTCTCATTTTGGATTATGTTGGCTGTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFF:FFFF,:FFFFFFFF,FFF,FFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFF:FFFF:,FFFFFFFFFFFNM:i:1MD:Z:139G11MC:Z:136M15SAS:i:146XS:i:79Yt:Z:UU A00126:224:H7HMWDSX3:3:1111:17526:19288145Test_seq15284845136M15S=152698-286TTGGCTTTGTATAAATGCCCTTTATTATGTTGAGGAATTTTCCTTCTATTCCTATTTTGCTGAGAGTTTTTATCATGAATGGGTGTTGAACTTTGTCAAATGCCCTTTTTGCATCAGTTGATAATATCTTGTGATTGATCACTTTTGGTAGFFFF,FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF::FFFFFF:FFFFFFFFF:FFFFFFFFFFF,FFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:136MC:Z:151MAS:i:136XS:i:116XA:Z:Test_seq,+40033,15S136M,4;Yt:Z:UU 60 45 ATCCCCTTTATTTCTTTATCTAGCCTAATTGCTCTGGCTAGGACCTCCAACACAGTGTTGAATAAGAACGGTGATAAAGGGCATCCTTGTCTGGGTCCCAGTCTCAGTGGGAATGCTTTCAGGCTCTCTCATTTTGGATTATGTTGGCTGT TTGGCTTTGTATAAATGCCCTTTATTATGTTGAGGAATTTTCCTTCTATTCCTATTTTGCTGAGAGTTTTTATCATGAATGGGTGTTGAACTTTGTCAAATGCCCTTTTTGCATCAGTTGATAATATCTTGTGATTGATCACTTTTGGTAG +A00126:224:H7HMWDSX3:3:1111:11062:21277 ! 0 Test_seq 170255 - + NR A00126:224:H7HMWDSX3:3:1111:11062:21277145Test_seq17047460129M22S=170255-348ATGTGTGTTGTTCGCAATTTCATTTTTCCACATACTTTTTTGTGCTGAGACATTTTTCTTAGTAAATTGTGAGATCTTCATTTTCATAGTGTTTGATTTTATGTTTGTTGAGTCGTTACGTTTTTCTTGGCTTTTATCTTGAGTTATGGAGFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:9MD:Z:12G2C0T34G12G11C11A5A1C32MC:Z:151MAS:i:84XS:i:0Yt:Z:NR A00126:224:H7HMWDSX3:3:1111:11062:2127797Test_seq17025560151M=170474348GCTTATTGTTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCGTGACTCTCTGTCTCTTTATTGGTGCATTFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFF:FFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:7A143MC:Z:129M22SAS:i:146XS:i:78Yt:Z:NR 0 60 GCTTATTGTTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCGTGACTCTCTGTCTCTTTATTGGTGCATT +A00126:224:H7HMWDSX3:3:1111:9127:26193 Test_seq 171173 Test_seq 171466 + - UU A00126:224:H7HMWDSX3:3:1111:9127:26193161Test_seq17117360151M=171316294GATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATGGGGTTCGATGAGCATCTTGGATAGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:F:FFFFFFFFF,FFFFFFFFF:FFFFFFFFFFFFFFFFF,F:,FFF::FF:FFFFFFFFF:FF,FFFFFF,FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:36Yt:Z:UU A00126:224:H7HMWDSX3:3:1111:9127:2619381Test_seq17131660151M=171173-294TTGGATAGATATCCTTTCGTCTTTCATGATGTCAGGGAAGTTTTGTGTCAGGAGTTCTTCAACTATTTTCTCTGTGTTTTCTCTCCCCCCTCCCTGTTCTGGGACTACAATCACCCGCAAGTTATCCTTCTTGATAGAGTCCCACATGATTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:5MD:Z:82G1G15T5C7T36MC:Z:151MAS:i:126XS:i:41Yt:Z:UU 60 60 GATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATGGGGTTCGATGAGCATCTTGGATAG TTGGATAGATATCCTTTCGTCTTTCATGATGTCAGGGAAGTTTTGTGTCAGGAGTTCTTCAACTATTTTCTCTGTGTTTTCTCTCCCCCCTCCCTGTTCTGGGACTACAATCACCCGCAAGTTATCCTTCTTGATAGAGTCCCACATGATT +A00126:224:H7HMWDSX3:3:1111:3956:26381 Test_seq 168615 Test_seq 212799 - - UU A00126:224:H7HMWDSX3:3:1111:3956:26381177Test_seq16846560151M=21264944185CGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTFFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:30T120MC:Z:151MAS:i:146XS:i:91Yt:Z:UU A00126:224:H7HMWDSX3:3:1111:3956:26381113Test_seq21264927151M=168465-44185GGCAAGGAAGAAGTAAAGTTATCACTATTTGCAGATGACATGATTATATACACAGAAAACCCTAAGGAATCCTCCAGAAAACTACTGAAACTAATAGAAGAGTTTGGCAGAGTCTCAGGTTATAAAATAAACATACAAAAATCACTTGGATFFF:,:FF:FFF:FFFFFFFFFF:FF,FFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:FFFFFFFFFFFNM:i:7MD:Z:8G8A5T20C0T17T61G25MC:Z:151MAS:i:116XS:i:101XA:Z:Test_seq,+168239,151M,10;Yt:Z:UU 60 27 CGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTT GGCAAGGAAGAAGTAAAGTTATCACTATTTGCAGATGACATGATTATATACACAGAAAACCCTAAGGAATCCTCCAGAAAACTACTGAAACTAATAGAAGAGTTTGGCAGAGTCTCAGGTTATAAAATAAACATACAAAAATCACTTGGAT +A00126:224:H7HMWDSX3:3:1111:9986:26334 Test_seq 167568 Test_seq 213148 + + UU A00126:224:H7HMWDSX3:3:1111:9986:26334129Test_seq1675689151M=21314845581GATCGACTTGCAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAGAAGACTATCATTTCCCCAATTGACTGACACTGGTCCTTTGTCAAATATCAGCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFF:FFFFFFF:FFFFF,FFFFFFFFFFFFFFFFF:FFFFFFFNM:i:3MD:Z:4C5G87A52MC:Z:151MAS:i:136XS:i:130XA:Z:Test_seq,-213320,140M2I9M,5;Yt:Z:UU A00126:224:H7HMWDSX3:3:1111:9986:2633465Test_seq2131483151M=167568-45581GCAAAGCATTACTGAAAAAGAAGAAGAAAGTGGGAGGCCTCACTCTACCTGATTTCAGAACCTATTATACAGCTACAGTAGTCAAAACAGCCTGGTACTGGTACAACAACAGGCACATAGACCAATGGAACAGAATTGAGAACCCAGATATFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:1T149MC:Z:151MAS:i:149XS:i:146XA:Z:Test_seq,-167740,151M,1;Yt:Z:UU 9 3 GATCGACTTGCAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAGAAGACTATCATTTCCCCAATTGACTGACACTGGTCCTTTGTCAAATATCAGC GCAAAGCATTACTGAAAAAGAAGAAGAAAGTGGGAGGCCTCACTCTACCTGATTTCAGAACCTATTATACAGCTACAGTAGTCAAAACAGCCTGGTACTGGTACAACAACAGGCACATAGACCAATGGAACAGAATTGAGAACCCAGATAT +A00126:224:H7HMWDSX3:3:1111:3043:26428 Test_seq 170295 Test_seq 170762 + - UU A00126:224:H7HMWDSX3:3:1111:3043:2642897Test_seq17029560151M=170622468TTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCTGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAATAAGTT:FF:FFFFFFFF,FF:FFFFFFFFFFFFFF,:FFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:FFFFFFFFFFFFFF:F:FFF:FFFFFFFF:FFFFF,:FF,FF:FFFFFFFFFF,FF,FFFFFFFFF,FFFFFF,:F,FFFFFFFF:FFNM:i:1MD:Z:81C69MC:Z:141M10SAS:i:146XS:i:78Yt:Z:UU A00126:224:H7HMWDSX3:3:1111:3043:26428145Test_seq17062260141M10S=170295-468CTTTTTGTGGTTACCTTATTATTTACCCCTATTTTTCTAAGTAAAAACCTAACTTGTATCGTTCTATATCGCCTTGTATCACTCTCCATATGGCAGTTCAATGCCTCCTGTATTTAGTCCCTCTTTTTGATTATTGTGATCTCTTACCTATF,F,FFFF,FFFF,FFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFF:::FFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFFNM:i:3MD:Z:63G6A18C51MC:Z:151MAS:i:126XS:i:37Yt:Z:UU 60 60 TTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCTGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAATAAGTT CTTTTTGTGGTTACCTTATTATTTACCCCTATTTTTCTAAGTAAAAACCTAACTTGTATCGTTCTATATCGCCTTGTATCACTCTCCATATGGCAGTTCAATGCCTCCTGTATTTAGTCCCTCTTTTTGATTATTGTGATCTCTTACCTAT +A00126:224:H7HMWDSX3:3:1111:10646:27853 Test_seq 167568 Test_seq 213148 + + UU A00126:224:H7HMWDSX3:3:1111:10646:27853129Test_seq1675689151M=21314845581GATCGACTTGCAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAGAAGACTATCATTTCCCCAATTGACTGACACTGGTCCTTTGTCAAATATCAGCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF,F:FF,FF:FFFFFF,FFF:F:FFF:F:FFFFFFFFFF::,FFFFF,NM:i:3MD:Z:4C5G87A52MC:Z:151MAS:i:136XS:i:130XA:Z:Test_seq,-213320,140M2I9M,5;Yt:Z:UU A00126:224:H7HMWDSX3:3:1111:10646:2785365Test_seq2131483151M=167568-45581GCAAAGCATTACTGAAAAAGAAGAAGAAAGTGGGAGGCCTCACTCTACCTGATTTCAGAACCTATTATACAGCTACAGTAGTCAAAACAGCCTGGTACTGGTACAACAACAGGCACATAGACCAATGGAACAGAATTGAGAACCCAGATATFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFFFF,FFFFFNM:i:1MD:Z:1T149MC:Z:151MAS:i:149XS:i:146XA:Z:Test_seq,-167740,151M,1;Yt:Z:UU 9 3 GATCGACTTGCAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAGAAGACTATCATTTCCCCAATTGACTGACACTGGTCCTTTGTCAAATATCAGC GCAAAGCATTACTGAAAAAGAAGAAGAAAGTGGGAGGCCTCACTCTACCTGATTTCAGAACCTATTATACAGCTACAGTAGTCAAAACAGCCTGGTACTGGTACAACAACAGGCACATAGACCAATGGAACAGAATTGAGAACCCAGATAT +A00126:224:H7HMWDSX3:3:1111:23954:28009 Test_seq 166002 Test_seq 166356 + - UU A00126:224:H7HMWDSX3:3:1111:23954:2800997Test_seq16600260151M=166206355ATTCCTTTAGTTGCAGTCATACCATTAGGTATGGTCTTTTCATGAGAATTTGGGGTCTGTTTCCCATTGGTCTCCTGCTCCCTCAGGAGTTGTCTGTTGTGCTCCCTGACAGGGCAGACATCGATTGTGGCCGGGCACCAACTAGTTCTTCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFNM:i:7MD:Z:6C4C0A2T5T18T19A90MC:Z:151MAS:i:120XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1111:23954:28009145Test_seq16620660151M=166002-355GTTCTTAGTTGTCGTGTGACCTTGGTGTTCTTCCTTTGCCTTTGCTCCAGGTGGGTTGAGACCAATTGATGTATCTTAGATGGCCGCTTGTTGGCATTTAGGACCCCAGGTGCCACAATTCAAAGTGGGATGCAGAATGTTTTCATAATAGFFFFFFFFFFFFFFFFFFFFFFFFFFF,FF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:18G132MC:Z:151MAS:i:146XS:i:81Yt:Z:UU 60 60 ATTCCTTTAGTTGCAGTCATACCATTAGGTATGGTCTTTTCATGAGAATTTGGGGTCTGTTTCCCATTGGTCTCCTGCTCCCTCAGGAGTTGTCTGTTGTGCTCCCTGACAGGGCAGACATCGATTGTGGCCGGGCACCAACTAGTTCTTC GTTCTTAGTTGTCGTGTGACCTTGGTGTTCTTCCTTTGCCTTTGCTCCAGGTGGGTTGAGACCAATTGATGTATCTTAGATGGCCGCTTGTTGGCATTTAGGACCCCAGGTGCCACAATTCAAAGTGGGATGCAGAATGTTTTCATAATAG +A00126:224:H7HMWDSX3:3:1111:22354:28025 Test_seq 169011 Test_seq 169427 + - UU A00126:224:H7HMWDSX3:3:1111:22354:28025161Test_seq16901160151M=169277417GATTTGGTAGAACTCTGCAGTGAAGCCGTCCAGGCCAGGACTTTTTTTTGTTGGAAGTTTTTTGATTACTGTTTCAATCTCTTTTTTTGTTATGGGTCTATTTAGTTGTTCTACTTCTGAATGTGTTAGTTTAGGTAGGTAGTGTTTTTCCFFFFFFFFFFFFFF:FFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:FFFF,FFFFF,:FFFFFFF,F,F:FFFFFFFFF:FFFFFFFFFFF,FFFFFFFFF,FFFF:FFFFFFFFFFNM:i:5MD:Z:1G28T0G37C80G0MC:Z:151MAS:i:133XS:i:58Yt:Z:UU A00126:224:H7HMWDSX3:3:1111:22354:2802581Test_seq16927760151M=169011-417TTCTCATTCGGGTTATTTGTTTCCTTTCCTGTTTTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCFFFFFFFFFFFFFFFFFF::FF:FFFFF:FFFFFFFFFFFFFFFFFFFFFF,FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFF:FFNM:i:1MD:Z:4T146MC:Z:151MAS:i:146XS:i:98Yt:Z:UU 60 60 GATTTGGTAGAACTCTGCAGTGAAGCCGTCCAGGCCAGGACTTTTTTTTGTTGGAAGTTTTTTGATTACTGTTTCAATCTCTTTTTTTGTTATGGGTCTATTTAGTTGTTCTACTTCTGAATGTGTTAGTTTAGGTAGGTAGTGTTTTTCC TTCTCATTCGGGTTATTTGTTTCCTTTCCTGTTTTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGC +A00126:224:H7HMWDSX3:3:1111:9661:28714 Test_seq 125729 Test_seq 126089 + - UU A00126:224:H7HMWDSX3:3:1111:9661:2871497Test_seq12572960151M=125939361TTTGCAGCTTTCCCAAGGCAGAGCAAAGCACGAGAAGATGGGCTCACAGGCCCGATCGCAGGGGCTCACAGCCTCCTCACTCTCCTAGGTTGCATTTCACAGACCAGCTTCCCTCACCCAGGATACTGGAGGCCGCAGCCTTCCAAATCAGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1111:9661:28714145Test_seq12593960151M=125729-361GTGATTAACCTCATTAGCACACGTGGTTGCCTCAGATGGCATTAGCAGTCAGCCAGATATTATTAAAAACAACAGCCATTGCCCTGTTGGGTGCACCGTGCCTGGTGCTCCCTAGCTCTGGGCCTTCAGGAGCAGCTGGTCACACACCTTAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU 60 60 TTTGCAGCTTTCCCAAGGCAGAGCAAAGCACGAGAAGATGGGCTCACAGGCCCGATCGCAGGGGCTCACAGCCTCCTCACTCTCCTAGGTTGCATTTCACAGACCAGCTTCCCTCACCCAGGATACTGGAGGCCGCAGCCTTCCAAATCAG GTGATTAACCTCATTAGCACACGTGGTTGCCTCAGATGGCATTAGCAGTCAGCCAGATATTATTAAAAACAACAGCCATTGCCCTGTTGGGTGCACCGTGCCTGGTGCTCCCTAGCTCTGGGCCTTCAGGAGCAGCTGGTCACACACCTTA +A00126:224:H7HMWDSX3:3:1111:19542:29011 Test_seq 170945 Test_seq 171324 + - UU A00126:224:H7HMWDSX3:3:1111:19542:2901197Test_seq1709453998M1D53M=171174380CAAACAATATCCTTTAGTATTTCTTGTAGCTTTGGTTTGTTTTTTTCAAATTCTCTAAACTTGTGTTTGTCTGTAAATATCTTAATTTTGCATTCATATTCAGAGAGAGTTTTGCTGGATATATGATCCTTGGCTGGCAGTTTTTATCCTTFFFFFFF:FFFF,FFFFFF:FF:F:FF:FF,FF:FFF:FF,FFFF,FFFF,:,FFFFFFFFFFF,FFFFFF,FFFFFFF::FFF:FFFF:F,,FFFFF,F,FFF,FFFFFFF,FFFFFFFFFF:,FF:FF:F,FF:FFF,F:,FF,:F:FFNM:i:16MD:Z:8C5A2C8C2T0C7A0G5G22A19C0T1C6^T3C31T17MC:Z:151MAS:i:69XS:i:40Yt:Z:UU A00126:224:H7HMWDSX3:3:1111:19542:29011145Test_seq17117460151M=170945-380ATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATGGGGTTCGATGAGCATCTTGGATAGAFFFFFFFFF:F,FFFFFFFFFFFFF,FFFF:FFFFFFFFFFFFFFF:FFFFFFFFF,FFFFFF,FFFF:FFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:98M1D53MAS:i:151XS:i:37Yt:Z:UU 39 60 CAAACAATATCCTTTAGTATTTCTTGTAGCTTTGGTTTGTTTTTTTCAAATTCTCTAAACTTGTGTTTGTCTGTAAATATCTTAATTTTGCATTCATATTCAGAGAGAGTTTTGCTGGATATATGATCCTTGGCTGGCAGTTTTTATCCTT ATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATGGGGTTCGATGAGCATCTTGGATAGA +A00126:224:H7HMWDSX3:3:1111:27706:33755 Test_seq 138290 Test_seq 138706 + - UU A00126:224:H7HMWDSX3:3:1111:27706:3375597Test_seq13829060151M=138556417GCTGAGTAACCAGTAGTCTATTAAGAAGGCACATATGGTGCTAGAATAACTAGAAAATAGTGCTGGAAAAACTGCATATCCATATGGAAAAAATGAACCTTAACCCTTACTTCACACCATATATAAAAACCAATTCAAAACAGATCTTAGAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::F:FFFFFFFFF:NM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1111:27706:33755145Test_seq13855660151M=138290-417AAGAACAGGAAAGGCACCTTTATTTTGTTGTACAAGGAAAAGGAGTCAGTCGGAGCTGTTGCGCTAAGACTGCTCCCCAAGGACAAGGGGAAAAGTTACTTTTAAGGGGTTTACAAGAAGGGAGTTCATACGAGTTATATCAGCAACATTCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU 60 60 GCTGAGTAACCAGTAGTCTATTAAGAAGGCACATATGGTGCTAGAATAACTAGAAAATAGTGCTGGAAAAACTGCATATCCATATGGAAAAAATGAACCTTAACCCTTACTTCACACCATATATAAAAACCAATTCAAAACAGATCTTAGA AAGAACAGGAAAGGCACCTTTATTTTGTTGTACAAGGAAAAGGAGTCAGTCGGAGCTGTTGCGCTAAGACTGCTCCCCAAGGACAAGGGGAAAAGTTACTTTTAAGGGGTTTACAAGAAGGGAGTTCATACGAGTTATATCAGCAACATTC +A00126:224:H7HMWDSX3:3:1111:8910:34209 ! 0 Test_seq 167708 - - NR A00126:224:H7HMWDSX3:3:1111:8910:3420997Test_seq1675315931S120M=167558178GCCATCTTGGTAACTCTTTGGAAACTATGACTGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:NM:i:0MD:Z:120MC:Z:151MAS:i:120XS:i:95Yt:Z:NR A00126:224:H7HMWDSX3:3:1111:8910:34209145Test_seq16755832151M=167531-178TTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATCATTTCCCCAATTGACTGACACTGGTCCTTTGTCFFFFFFFFFFFFFFF:FFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:31S120MAS:i:151XS:i:136XA:Z:Test_seq,+213330,130M2I19M,3;Yt:Z:NR 0 32 TTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATCATTTCCCCAATTGACTGACACTGGTCCTTTGTC +A00126:224:H7HMWDSX3:3:1111:24777:34413 Test_seq 417145 Test_seq 417607 + - UU A00126:224:H7HMWDSX3:3:1111:24777:3441397Test_seq41714560151M=417457463CATCTTTCTTACATTTCAAAGTCTGCTCCCTGGTTAAAGGAAGTGGAAATAAAGCGTGGGATTTGGGGATTAAATCAGTGGGATTCCAAATTAGGCTTATTTTTTTGTGGTGGTTATTCAATTACATAAATCTGTACTCTTGATCTTCTCAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1111:24777:34413145Test_seq41745760151M=417145-463TTCCAAAATCCAGAAGTTCTGGAAAAGAGATTTTTTTCTCGGCAGCCCCAAAATCCAAAAAATTTTTGGAGGAAAAAAAAAGTCCTTTGTAAGGTTGGCACCAAAACTTAGTCGACTGCAAAAACCTGACTTGAACTGATGTTCACACTTAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:46T104MC:Z:151MAS:i:146XS:i:0Yt:Z:UU 60 60 CATCTTTCTTACATTTCAAAGTCTGCTCCCTGGTTAAAGGAAGTGGAAATAAAGCGTGGGATTTGGGGATTAAATCAGTGGGATTCCAAATTAGGCTTATTTTTTTGTGGTGGTTATTCAATTACATAAATCTGTACTCTTGATCTTCTCA TTCCAAAATCCAGAAGTTCTGGAAAAGAGATTTTTTTCTCGGCAGCCCCAAAATCCAAAAAATTTTTGGAGGAAAAAAAAAGTCCTTTGTAAGGTTGGCACCAAAACTTAGTCGACTGCAAAAACCTGACTTGAACTGATGTTCACACTTA +A00126:224:H7HMWDSX3:3:1111:27832:34663 Test_seq 167854 Test_seq 168347 + - UU A00126:224:H7HMWDSX3:3:1111:27832:3466397Test_seq16785460151M=168197494CCTCCCACTTTCTTCTTCTTTTTCAGTAATGCTTTGCTTATCCGGGGGTTCTTTCCCTTGCATATGAAATTAGTGATTTGTTTCTCTATCCCCTTAAAATATGACATTGGTATTTGGATTGGAAGTGCGTTATATGTATAGATGGCTTTTGFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:59C91MC:Z:151MAS:i:146XS:i:59Yt:Z:UU A00126:224:H7HMWDSX3:3:1111:27832:34663145Test_seq16819737151M=167854-494TGGTTATTTCCTCTTCGGTGTTCTTTTTGTTGATGTAGAGGAATCCAAGTGATTTTTGTATGTTTATTTTATAACCTGAGACTCTTCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGGTTTTCTACGTATACGATFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFNM:i:4MD:Z:74T64C1T7G1MC:Z:151MAS:i:134XS:i:115XA:Z:Test_seq,+212691,151M,8;Yt:Z:UU 60 37 CCTCCCACTTTCTTCTTCTTTTTCAGTAATGCTTTGCTTATCCGGGGGTTCTTTCCCTTGCATATGAAATTAGTGATTTGTTTCTCTATCCCCTTAAAATATGACATTGGTATTTGGATTGGAAGTGCGTTATATGTATAGATGGCTTTTG TGGTTATTTCCTCTTCGGTGTTCTTTTTGTTGATGTAGAGGAATCCAAGTGATTTTTGTATGTTTATTTTATAACCTGAGACTCTTCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGGTTTTCTACGTATACGAT +A00126:224:H7HMWDSX3:3:1111:8802:34773 ! 0 Test_seq 168590 - - NR A00126:224:H7HMWDSX3:3:1111:8802:34773161Test_seq1683476029S122M=168440244TTATCCCCAATTTAGGTTTATTTGATCGATCATGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCTTGCCAATCCGGATACCCTTTATTTCTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGCGGTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFFNM:i:2MD:Z:18A28T74MC:Z:151MAS:i:112XS:i:66Yt:Z:NR A00126:224:H7HMWDSX3:3:1111:8802:3477381Test_seq16844060151M=168347-244CTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCTGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:29S122MAS:i:151XS:i:83Yt:Z:NR 0 60 CTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCTGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCC +A00126:224:H7HMWDSX3:3:1111:1570:34773 Test_seq 72280 Test_seq 72638 + - UU A00126:224:H7HMWDSX3:3:1111:1570:3477397Test_seq7228060151M=72488359ATCAGTCTGTTTTGAGATAGAAAAGAGATTAAACAAGGAGATTAAAGAAGCAGCGATGGAGGAAGAGAGATGCAAGGCCGCATGAAGGTCGCCCAAGAGCAGAAGCTCAAAAGAGGCTAAGGTCTTCCTCCAGAGCTGAGATAGAAAGCTTFFFF:FFFFFFFFFFFFFF:FFFFF::FFFFF:FFF:FFFFFFFF:FFFFFFFFFF:FF:FFFF::FF:FFF:FFFF,FFFFFFFFFFFF::FFFF::FFFF:FFFFFFF,F:,FF:FF:FFFFFFFF:FFFFFF:FFFFFFFFFF:FF:FNM:i:1MD:Z:137A13MC:Z:151MAS:i:146XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1111:1570:34773145Test_seq7248860151M=72280-359AAATTTGTATGTTAAAACCGCCTACTTGTGGTATTTCTGTTATAGAACACTGGATAACTAGGACAGTTGCCGTGAAGGGGCAGCCGACTCGATGGCAGCCAAAAACAACAACAACAGCAGTGAAACACAGGTAGTGTTTCTTGGATTCATTF:FFFFFFF:,F:FFFFFF:,FFFFFF,F,F:,FF:FFFFFFFFFFFFFFF,FFFFFFFF:FFFF:FFFFFFFF:FFFFFFF:F,FFFF::FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,:FFFFF:F:::FF:FFFFFF:NM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU 60 60 ATCAGTCTGTTTTGAGATAGAAAAGAGATTAAACAAGGAGATTAAAGAAGCAGCGATGGAGGAAGAGAGATGCAAGGCCGCATGAAGGTCGCCCAAGAGCAGAAGCTCAAAAGAGGCTAAGGTCTTCCTCCAGAGCTGAGATAGAAAGCTT AAATTTGTATGTTAAAACCGCCTACTTGTGGTATTTCTGTTATAGAACACTGGATAACTAGGACAGTTGCCGTGAAGGGGCAGCCGACTCGATGGCAGCCAAAAACAACAACAACAGCAGTGAAACACAGGTAGTGTTTCTTGGATTCATT +A00126:224:H7HMWDSX3:3:1111:14064:36808 Test_seq 167148 Test_seq 401034 + - UU A00126:224:H7HMWDSX3:3:1111:14064:3680897Test_seq16714842151M=400900233887AATTTGCATTTCTCTAATGGCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF::F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFFF,,F:FFF,,FFF:FFFFFFFFFFFF:FFFFFFFFFF:FF:FFFFFNM:i:1MD:Z:95T55MC:Z:135M16SAS:i:146XS:i:126XA:Z:Test_seq,-213736,151M,5;Yt:Z:UU A00126:224:H7HMWDSX3:3:1111:14064:36808145Test_seq4009004135M16S=167148-233887TTATCGGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATACCTTGTATTAGGGCTCCTAGGGTTGTCCCAATTTTTTCTTCCATGATCTTTATCATTTTAGTCTTTATGTTTAGGTCTTTGATCGATCCCTTCCCCACTC,FF:FFFF,FFFFFFFF,FFFF:FF:FF,F:FFFFFFFFF:FFFFF:FF:FFF:FFFFF:FFFFFFFFFFFFFFFF,FFFFFFFFFFFF:FFF:FFFFFFFF:FFFF:FFFFFFFF::FFFFFFFFFFFFFFFFFFFFFFFF:FFFF,F:FNM:i:7MD:Z:4T0T11G32G19A0C8T54MC:Z:151MAS:i:104XS:i:100XA:Z:Test_seq,-167437,135M16S,7;Yt:Z:UU 42 4 AATTTGCATTTCTCTAATGGCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGA TTATCGGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATACCTTGTATTAGGGCTCCTAGGGTTGTCCCAATTTTTTCTTCCATGATCTTTATCATTTTAGTCTTTATGTTTAGGTCTTTGATCGATCCCTTCCCCACTC +A00126:224:H7HMWDSX3:3:1112:23149:2597 Test_seq 169079 Test_seq 169468 + - UU A00126:224:H7HMWDSX3:3:1112:23149:259797Test_seq1690793739M4I108M=169318390CCGTTTCAATCTCTTTTTTTGTTATGGGTCTATTTAGTTGATCGATCTGCTTCTGAATGTGTTAGTTTAGGTAGGTAGTGTTTTTCAAGGAATTCATCCATTTCTTCTAGGTTTTCAAATTTGTTAGAGTACAATTTTTCATAATAATCTGFFF:FFF:FFFFF,FFFFFFFFFFFFFFF:F:FFFFFFFFFFF,FFFFFFF:FF:F:F:F::FFFFFF:FFFFF:FFFFFFF:FFFFF,FFF,F:FFFFFFFFFFFFFFF,FF:F:FFFFFFF:FF,FF,FFFFFF,:,,F:F:,F:F,:FNM:i:12MD:Z:40T3A37G1A37C11A1G2G7MC:Z:151MAS:i:99XS:i:76Yt:Z:UU A00126:224:H7HMWDSX3:3:1112:23149:2597145Test_seq16931860151M=169079-390GTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGATFFFFFFFFFFFFFFFFFF,FFFF:FFF:FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFNM:i:1MD:Z:149G1MC:Z:39M4I108MAS:i:149XS:i:112Yt:Z:UU 37 60 CCGTTTCAATCTCTTTTTTTGTTATGGGTCTATTTAGTTGATCGATCTGCTTCTGAATGTGTTAGTTTAGGTAGGTAGTGTTTTTCAAGGAATTCATCCATTTCTTCTAGGTTTTCAAATTTGTTAGAGTACAATTTTTCATAATAATCTG GTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGAT +A00126:224:H7HMWDSX3:3:1112:10447:5243 Test_seq 168427 Test_seq 168802 + - RU A00126:224:H7HMWDSX3:3:1112:10447:5243161Test_seq16842760151M=168773376GCCCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCAGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:68T82MC:Z:121S30MAS:i:146XS:i:43Yt:Z:RU A00126:224:H7HMWDSX3:3:1112:10447:52432129Test_seq16855560121M30H=168427-249TGCATAGATGCCCTTTATTATGTTGCGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATCFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:25A95MC:Z:151MAS:i:116XS:i:70SA:Z:Test_seq,168773,-,121S30M,60,0;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1112:10447:524381Test_seq16877360121S30M=168427-376TGCATAGATGCCCTTTATTATGTTGCGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATCGATCAGGGTGAATTATTTTTTTGATGTGTTFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFNM:i:0MD:Z:30MC:Z:151MAS:i:30XS:i:0SA:Z:Test_seq,168555,-,121M30S,60,1;Yt:Z:RU 60 60 GCCCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCAGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGT TGCATAGATGCCCTTTATTATGTTGCGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATCGATCAGGGTGAATTATTTTTTTGATGTGTT +A00126:224:H7HMWDSX3:3:1112:21884:7012 Test_seq 167977 Test_seq 168320 + - UU A00126:224:H7HMWDSX3:3:1112:21884:701297Test_seq16797726151M=168170344AGTGCGTTAAATGTATAGATGGCTTTTGGTAGAATAGACATTTTTACTATGTTAAGTCTTCCTATCCATGAGCAAGGTATGTTTTTCCACTTAAGTATGTCCTTTTGAATTTCTTGCAGTAGAGCTTTGTAGTTTTCTTTGTATAGGTCTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFF:FFFFFNM:i:8MD:Z:9T64G8C32T2C4T3A13G8MC:Z:151MAS:i:111XS:i:96XA:Z:Test_seq,-212911,141M10S,9;Yt:Z:UU A00126:224:H7HMWDSX3:3:1112:21884:7012145Test_seq16817031151M=167977-344TGGGGGCTACTGTGAATGGTATTGATTTGGTTATTTCCTCTTCGGTGTTCTTTTTGTTGATGTAGAGGAATCCAAGTGATTTTTGTATGTTTATTTTATAACCTGAGACTCTTCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:101T49MC:Z:151MAS:i:146XS:i:131XA:Z:Test_seq,+212718,151M,4;Yt:Z:UU 26 31 AGTGCGTTAAATGTATAGATGGCTTTTGGTAGAATAGACATTTTTACTATGTTAAGTCTTCCTATCCATGAGCAAGGTATGTTTTTCCACTTAAGTATGTCCTTTTGAATTTCTTGCAGTAGAGCTTTGTAGTTTTCTTTGTATAGGTCTT TGGGGGCTACTGTGAATGGTATTGATTTGGTTATTTCCTCTTCGGTGTTCTTTTTGTTGATGTAGAGGAATCCAAGTGATTTTTGTATGTTTATTTTATAACCTGAGACTCTTCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGA +A00126:224:H7HMWDSX3:3:1112:16595:8124 Test_seq 170144 Test_seq 170457 + - UU A00126:224:H7HMWDSX3:3:1112:16595:8124161Test_seq1701446016S135M=170307314TTTTATGTTATTGATCGATCAATTGTCCCTTTTATCATTATATAGTGTCCTTCTTTATCCTTTGTGGTGGATTTAAGTCTAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCCTCTTCTTTTTTGCTTATTGTTTGCTTGATATATTTFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:F:FFFFFFFFF:FFFFFF:FFFFFFFF:FFFFFFFFF:FFF,FFFFFFFF:FFFFFF:F,FFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFF,FNM:i:3MD:Z:51C0A65A16MC:Z:151MAS:i:120XS:i:53Yt:Z:UU A00126:224:H7HMWDSX3:3:1112:16595:812481Test_seq17030760151M=170144-314TGTGTCTCTAAGTCTACGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAATAAGTTTTTAGTGCTGTCFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FNM:i:1MD:Z:16A134MC:Z:16S135MAS:i:146XS:i:48Yt:Z:UU 60 60 TTTTATGTTATTGATCGATCAATTGTCCCTTTTATCATTATATAGTGTCCTTCTTTATCCTTTGTGGTGGATTTAAGTCTAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCCTCTTCTTTTTTGCTTATTGTTTGCTTGATATATTT TGTGTCTCTAAGTCTACGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAATAAGTTTTTAGTGCTGTC +A00126:224:H7HMWDSX3:3:1112:9209:9361 Test_seq 212679 Test_seq 212953 + - UU A00126:224:H7HMWDSX3:3:1112:9209:936197Test_seq21267960151M=212803275GCAGATGACATGATCTTATACACAGAAAACCCTAAGGAATCCTCCAGAAAACTACTGAAACTAATAGAAGAGTTTGGCAGAGTCTCAGGTTATAAGATAAACATACAAAAATCACTTGGATTCCTCTACATCAACAAAAAGAACATCAAAGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFNM:i:1MD:Z:33T117MC:Z:151MAS:i:146XS:i:110Yt:Z:UU A00126:224:H7HMWDSX3:3:1112:9209:9361145Test_seq21280358151M=212679-275TCTACATCAACAAAAAGAACATCAAAGAGGAAATCACCAAATCAATACCATTCACAGTAGCCCCCAAGAAGAGAAAATACTTAGGAATAAATCTTACCAAAGATGTAAAAGACCTATACAAAGAAAACTACAAAGCACTACTACAAGAAATFFFFFFF:FFFFFFFFFFFFFFFFFFFF:::FFFF,F,FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFNM:i:5MD:Z:34A37T54T7T14C0MC:Z:151MAS:i:130XS:i:100Yt:Z:UU 60 58 GCAGATGACATGATCTTATACACAGAAAACCCTAAGGAATCCTCCAGAAAACTACTGAAACTAATAGAAGAGTTTGGCAGAGTCTCAGGTTATAAGATAAACATACAAAAATCACTTGGATTCCTCTACATCAACAAAAAGAACATCAAAG TCTACATCAACAAAAAGAACATCAAAGAGGAAATCACCAAATCAATACCATTCACAGTAGCCCCCAAGAAGAGAAAATACTTAGGAATAAATCTTACCAAAGATGTAAAAGACCTATACAAAGAAAACTACAAAGCACTACTACAAGAAAT +A00126:224:H7HMWDSX3:3:1112:16875:9518 Test_seq 397769 Test_seq 398168 + - UU A00126:224:H7HMWDSX3:3:1112:16875:9518161Test_seq39776960151M=398018400CGTCTTTTGTGTGCCTTCCAACCTGAGGGGCTCACCTTACTGTACTATATCAGACAATGTTCTGCTGCTATTCATCAGCTTTTCAGTGGCCTAATTTTTTTCAGAAGTAGATCACCAGGTCCTTCTTCCCAGTCTGTCTTAGTCTGGAAGCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:FNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1112:16875:951881Test_seq39801860151M=397769-400GACAAACTGACAGACTAGTGGTGGATTTCTAAATCATACCACTCAAAAAGCCATATATTATCATGTGAAATTTTTTTTCACAGTTTATGAATGAAACTTTTTCTTTCTACCCTTGCCAACACTGAATAGTATCAAGATTTTTCAATTTGCCFFF:FFFFFFFFFFF:FFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:134C16MC:Z:151MAS:i:146XS:i:0Yt:Z:UU 60 60 CGTCTTTTGTGTGCCTTCCAACCTGAGGGGCTCACCTTACTGTACTATATCAGACAATGTTCTGCTGCTATTCATCAGCTTTTCAGTGGCCTAATTTTTTTCAGAAGTAGATCACCAGGTCCTTCTTCCCAGTCTGTCTTAGTCTGGAAGC GACAAACTGACAGACTAGTGGTGGATTTCTAAATCATACCACTCAAAAAGCCATATATTATCATGTGAAATTTTTTTTCACAGTTTATGAATGAAACTTTTTCTTTCTACCCTTGCCAACACTGAATAGTATCAAGATTTTTCAATTTGCC +A00126:224:H7HMWDSX3:3:1112:10357:9721 Test_seq 212679 Test_seq 212953 + - UU A00126:224:H7HMWDSX3:3:1112:10357:972197Test_seq21267960151M=212803275GCAGATGACATGATCTTATACACAGAAAACCCTAAGGAATCCTCCAGAAAACTACTGAAACTAATAGAAGAGTTTGGCAGAGTCTCAGGTTATAAGATAAACATACAAAAATCACTTGGATTCCTCTACATCAACAAAAAGAACATCAAAGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFNM:i:1MD:Z:33T117MC:Z:151MAS:i:146XS:i:110Yt:Z:UU A00126:224:H7HMWDSX3:3:1112:10357:9721145Test_seq21280358151M=212679-275TCTACATCAACAAAAAGAACATCAAAGAGGAAATCACCAAATCAATACCATTCACAGTAGCCCCCAAGAAGAGAAAATACTTAGGAATAAATCTTACCAAAGATGTAAAAGACCTATACAAAGAAAACTACAAAGCACTACTACAAGAAATF:FFFFFFFF,FFFFF:FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:5MD:Z:34A37T54T7T14C0MC:Z:151MAS:i:130XS:i:100Yt:Z:UU 60 58 GCAGATGACATGATCTTATACACAGAAAACCCTAAGGAATCCTCCAGAAAACTACTGAAACTAATAGAAGAGTTTGGCAGAGTCTCAGGTTATAAGATAAACATACAAAAATCACTTGGATTCCTCTACATCAACAAAAAGAACATCAAAG TCTACATCAACAAAAAGAACATCAAAGAGGAAATCACCAAATCAATACCATTCACAGTAGCCCCCAAGAAGAGAAAATACTTAGGAATAAATCTTACCAAAGATGTAAAAGACCTATACAAAGAAAACTACAAAGCACTACTACAAGAAAT +A00126:224:H7HMWDSX3:3:1112:22914:10739 Test_seq 167173 Test_seq 167663 + - UU A00126:224:H7HMWDSX3:3:1112:22914:10739161Test_seq16717350151M=167513491GATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAATCATGTAGATTTTAGAGATCAGGCF:FFF,FFFFFFFFFFFFF:FFFFF:::FF:FFFFF:FFFFFFFFFFFFFFFFFFFFF:FFFFF:,FFFFFFFFFFFFFFFFF:FFFF:FFFF,FFF:FFF:FF:FFFFFFF:,:FFFFFF,FFFFFFFF:FFFF:FFFFF,FFFFFF:FFNM:i:2MD:Z:70T79A0MC:Z:151MAS:i:145XS:i:121XA:Z:Test_seq,-213711,151M,6;Yt:Z:UU A00126:224:H7HMWDSX3:3:1112:22914:1073981Test_seq16751357151M=167173-491ATCCTATTTTTTCTTCCATGATCTTTATTGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:28C122MC:Z:151MAS:i:146XS:i:119XA:Z:Test_seq,+213375,85M2I23M2I37M2S,6;Yt:Z:UU 50 57 GATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAATCATGTAGATTTTAGAGATCAGGC ATCCTATTTTTTCTTCCATGATCTTTATTGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTT +A00126:224:H7HMWDSX3:3:1112:6008:14027 Test_seq 168347 Test_seq 168672 + - UU A00126:224:H7HMWDSX3:3:1112:6008:1402797Test_seq1683476018S133M=168522326GCTGCCAAATCACTGAGATCATGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCTTGCCAATCTGGATACCCTTTATTTCTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGCACGATGTTGAATAAGAGCGGTGATAAAGGGCAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF:FFFFFF:FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:2MD:Z:18A81T32MC:Z:151MAS:i:123XS:i:76Yt:Z:UU A00126:224:H7HMWDSX3:3:1112:6008:14027145Test_seq16852260151M=168347-326CTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCGTTTTCTGCATCTATTGATAAGFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:129C21MC:Z:18S133MAS:i:146XS:i:72Yt:Z:UU 60 60 GCTGCCAAATCACTGAGATCATGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCTTGCCAATCTGGATACCCTTTATTTCTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGCACGATGTTGAATAAGAGCGGTGATAAAGGGCA CTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCGTTTTCTGCATCTATTGATAAG +A00126:224:H7HMWDSX3:3:1112:27778:14184 Test_seq 170407 Test_seq 170660 + - UU A00126:224:H7HMWDSX3:3:1112:27778:1418497Test_seq17040760151M=170510254AGTCCATTTACATTCAGCGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTTTTCATGTGTGTTGTTGGCCTTTTCATTTTTCCACATGCTTTTTTGTGCTGAGACGTTTTTCTTGGTAGATTGTGAGATCCTCATTTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF,,FFF,FF,FFF,,,F:F::,,F,F:FF:FFFNM:i:2MD:Z:100A26A23MC:Z:151MAS:i:141XS:i:25Yt:Z:UU A00126:224:H7HMWDSX3:3:1112:27778:14184145Test_seq17051060151M=170407-254TTTTTGTGCTGAGACGTTTTTCTTGGTAGATTGTGAGATCCTCATTTTCATAATGTTTAACTTTATGTTTGTTGAGTCGTTACGTTTTTCTTGAGTTATGGAATTGATATTCCTTTTTGTGGTTACCTTATTATTTACCCCTATTTTTCTAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:24A126MC:Z:151MAS:i:146XS:i:26Yt:Z:UU 60 60 AGTCCATTTACATTCAGCGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTTTTCATGTGTGTTGTTGGCCTTTTCATTTTTCCACATGCTTTTTTGTGCTGAGACGTTTTTCTTGGTAGATTGTGAGATCCTCATTTT TTTTTGTGCTGAGACGTTTTTCTTGGTAGATTGTGAGATCCTCATTTTCATAATGTTTAACTTTATGTTTGTTGAGTCGTTACGTTTTTCTTGAGTTATGGAATTGATATTCCTTTTTGTGGTTACCTTATTATTTACCCCTATTTTTCTA +A00126:224:H7HMWDSX3:3:1112:5927:14763 Test_seq 366670 Test_seq 367055 + - UU A00126:224:H7HMWDSX3:3:1112:5927:1476397Test_seq36667060151M=366905386AGTGTTAAAAAGCAAAGATGTTACCTTGAAGACTAAGGTGTGCCTGACCGAAAAAAAGACCGAAGCCATGGTATTTTCAATTGCATCATATGCATGTGAAAGCTGGACAATGAATGAGAAAGGCCAAAGAAGAATTGATGTTTTTGAATTAFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:50Yt:Z:UU A00126:224:H7HMWDSX3:3:1112:5927:14763145Test_seq36690560151M=366670-386TCCTTAGAAGCAAGATTGTCAAGACTTTGATGTACTTTGGGCATGTTATCAGGAAGGATTCATCCCTAGAGAAGGACACCATGCTTGGTAAAATAGAAAAAAACCAAAACCAAACCCACTGCCGTCAAGTGATTCCGACTCATAGCGATCTF:FFFFFFF::FF:FFFFFFFF:F,FFF,FFFFFFFFFFFFFFFFFFFFFFFFFFF,FFF,FFFF,FFFFFFFF,FFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:61G89MC:Z:151MAS:i:146XS:i:22Yt:Z:UU 60 60 AGTGTTAAAAAGCAAAGATGTTACCTTGAAGACTAAGGTGTGCCTGACCGAAAAAAAGACCGAAGCCATGGTATTTTCAATTGCATCATATGCATGTGAAAGCTGGACAATGAATGAGAAAGGCCAAAGAAGAATTGATGTTTTTGAATTA TCCTTAGAAGCAAGATTGTCAAGACTTTGATGTACTTTGGGCATGTTATCAGGAAGGATTCATCCCTAGAGAAGGACACCATGCTTGGTAAAATAGAAAAAAACCAAAACCAAACCCACTGCCGTCAAGTGATTCCGACTCATAGCGATCT +A00126:224:H7HMWDSX3:3:1112:9254:15922 Test_seq 260973 Test_seq 261025 + - UU A00126:224:H7HMWDSX3:3:1112:9254:1592297Test_seq260973853M=26097353GGGGTTCTCTGTTGTGTTCCCTGTCAGGGCAGTCATCTGTTGTAGAAGGGCACFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF,FF:FFFF,,,FFFFFNM:i:3MD:Z:37G7C0T6MC:Z:53MAS:i:40XS:i:36XA:Z:Test_seq,+347640,45M8S,2;Yt:Z:UU A00126:224:H7HMWDSX3:3:1112:9254:15922145Test_seq2609733453M=260973-53GGGGTTCTCTGTTGTGTTCCCTGTCAGGGCAGTCATCGGTTGTAGCTGGGCACFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:53MC:Z:53MAS:i:53XS:i:43XA:Z:Test_seq,-150325,53M,2;Yt:Z:UU 8 34 GGGGTTCTCTGTTGTGTTCCCTGTCAGGGCAGTCATCTGTTGTAGAAGGGCAC GGGGTTCTCTGTTGTGTTCCCTGTCAGGGCAGTCATCGGTTGTAGCTGGGCAC +A00126:224:H7HMWDSX3:3:1112:32723:16391 ! 0 Test_seq 171881 - - NR A00126:224:H7HMWDSX3:3:1112:32723:16391161Test_seq1716266027S124M=171731256TTCAATAAAAACCCAAATGAAATCGATTTAATCTTTTGGATTTCTACATGCTGTCTCTCTATGGATTCTTGCAACTTGTTAATTTTTCCACTATGTTCTTGAATAATCTTTTTGAGTTCTTCAACAGTTTTATCAGTGTGTTCCTTGGCTTFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFF:,FFFFFF,F:,FFFFF:FFFFFFFF:F,FFFFFFFFF:FF,FF,FFFFFF,FFFFFFFFFF,,:F:,FFFFF,F:FF:,,F,FFFNM:i:4MD:Z:50A14C13C11T32MC:Z:151MAS:i:104XS:i:71Yt:Z:NR A00126:224:H7HMWDSX3:3:1112:32723:1639181Test_seq17173160151M=171626-256TCAGTGTGTTCCTTGGCTTTTTCTGCATTTATCCTAATTTCATTTGTGATATCTTTAAGCATTCTGTAAATTAGTTTTTTATATTCTGTATCTGATAATTCCAGGATTGTATCTTCATTTGGGAAAGATTTTGATTCTTTTGTTTGGGGGGF,FF:FFF,FFF:FFFFFFFFFFFFFFF:F:FFFF,FFFFF:FFFFFFFFFFFFFFFFFFF,FFFFFFF,FFFFFFF:F:F:,:FFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFF:,FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFNM:i:1MD:Z:27G123MC:Z:27S124MAS:i:146XS:i:25Yt:Z:NR 0 60 TCAGTGTGTTCCTTGGCTTTTTCTGCATTTATCCTAATTTCATTTGTGATATCTTTAAGCATTCTGTAAATTAGTTTTTTATATTCTGTATCTGATAATTCCAGGATTGTATCTTCATTTGGGAAAGATTTTGATTCTTTTGTTTGGGGGG +A00126:224:H7HMWDSX3:3:1112:27633:17722 Test_seq 385678 Test_seq 388748 - - UR A00126:224:H7HMWDSX3:3:1112:27633:177222145Test_seq3883496024H127M=388598400ACTCCAGAATGAGGAGTGTTGAAGAGAGAATTATCTTATGAGGATAATTTTTGTTCAGAGTTTTCCTTAAAAGGCTTCTCTTTGAGTGCTCTCTGAAATGCTTTGCAGACTTTCTTCTGACGCACTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:127MC:Z:151MAS:i:127XS:i:0SA:Z:Test_seq,385655,-,127S24M,30,0;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1112:27633:17722113Test_seq38565530127S24M=3885983071AAGTGCGTCAGAAGAAAGTCTGCAAAGCATTTCAGAGAGCACTCAAAGAGAAGCCTTTTAAGGAAAACTCTGAACAAAAATTATCCTCATAAGATAATTCTCTCTTCAACACTCCTCATTCTGGAGTAATCACAGACTGTTCACACATTGCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFNM:i:0MD:Z:24MC:Z:151MAS:i:24XS:i:0SA:Z:Test_seq,388349,+,24S127M,60,0;Yt:Z:UR A00126:224:H7HMWDSX3:3:1112:27633:17722177Test_seq38859860151M=385655-3071AGATTGGCCATGAATGGGGTGATAAGAATAGGGAGAAGCACATACCAGTAGACCAGACTTCCTGTGCACAAGCCTTTGAATCCTGCTTCTCGCTTCTGAAATAGCATTCATCTTAGGGCTGGGATCATTTATTCTCTCTGGAATTAAGCAAFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:127S24MAS:i:151XS:i:0Yt:Z:UR 30 60 AAGTGCGTCAGAAGAAAGTCTGCAAAGCATTTCAGAGAGCACTCAAAGAGAAGCCTTTTAAGGAAAACTCTGAACAAAAATTATCCTCATAAGATAATTCTCTCTTCAACACTCCTCATTCTGGAGTAATCACAGACTGTTCACACATTGC AGATTGGCCATGAATGGGGTGATAAGAATAGGGAGAAGCACATACCAGTAGACCAGACTTCCTGTGCACAAGCCTTTGAATCCTGCTTCTCGCTTCTGAAATAGCATTCATCTTAGGGCTGGGATCATTTATTCTCTCTGGAATTAAGCAA +A00126:224:H7HMWDSX3:3:1112:25825:18098 ! 0 Test_seq 171611 - - NR A00126:224:H7HMWDSX3:3:1112:25825:18098161Test_seq1711746033S118M=171461438TATATTGAGTATATCATCATTTGACCTCAAAGAATTCTCCCTTGAAGGAAACCTTTTTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFF:FFFFFFF:FFFFFFFFFFFFF,F:FFFFFFFFFNM:i:1MD:Z:23C94MC:Z:151MAS:i:113XS:i:35Yt:Z:NR A00126:224:H7HMWDSX3:3:1112:25825:1809881Test_seq17146160151M=171174-438ATGATTCTTAGGGTTTCTTCATTTTTTTTAATTCTTTTATCTGATTTTTTTTCAGCTATGTTGGAGTTGATTCCCTGGTCCTCCAGAAGTCCCAGTCTACATTCTAATTGCTCGAGTCTGCTCCTCTGACTTTCTATTGCGTTGTCTAATTFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:122T28MC:Z:33S118MAS:i:146XS:i:39Yt:Z:NR 0 60 ATGATTCTTAGGGTTTCTTCATTTTTTTTAATTCTTTTATCTGATTTTTTTTCAGCTATGTTGGAGTTGATTCCCTGGTCCTCCAGAAGTCCCAGTCTACATTCTAATTGCTCGAGTCTGCTCCTCTGACTTTCTATTGCGTTGTCTAATT +A00126:224:H7HMWDSX3:3:1112:9362:18740 Test_seq 170285 Test_seq 170742 + - UU A00126:224:H7HMWDSX3:3:1112:9362:18740161Test_seq17028560151M=170592458ATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCGTGACTCTCTGTCTCCTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGAFFF,FFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFF:,FF::FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF,FFFF:FFFF:FFFFFFFFF:FFFFF:FFFF,F::FFF,F:FFFFFF,F,:FFFFFFFFF,FFF,FFFFFNM:i:1MD:Z:107T43MC:Z:151MAS:i:146XS:i:82Yt:Z:UU A00126:224:H7HMWDSX3:3:1112:9362:1874081Test_seq17059260151M=170285-458CTTTTTTCTTGAGTTATGGAATTGATATTCCTTTTTGTGGTTACCTTTTTATTTACCCCTATTTTTCTAAGTAAAAACCTAACTTGTATCCTTCTATTTCGCCTTGTATCACTCTCCATCTGGCAGTTCGATGCCTCCTATATTTAGTCCCFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFF:FF,FFFFFFFF:FFFF,FFFFF:F,FFFFFF:FFFFF:FFFF:FFFFFFF,FFFFFFFFFNM:i:8MD:Z:1G45A42G2G3A2A28A9G11MC:Z:151MAS:i:114XS:i:24Yt:Z:UU 60 60 ATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCGTGACTCTCTGTCTCCTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGA CTTTTTTCTTGAGTTATGGAATTGATATTCCTTTTTGTGGTTACCTTTTTATTTACCCCTATTTTTCTAAGTAAAAACCTAACTTGTATCCTTCTATTTCGCCTTGTATCACTCTCCATCTGGCAGTTCGATGCCTCCTATATTTAGTCCC +A00126:224:H7HMWDSX3:3:1112:31421:19304 Test_seq 236629 Test_seq 237126 + - UU A00126:224:H7HMWDSX3:3:1112:31421:1930497Test_seq23662960151M=236976498AAGAAAAATAAAAGTAAATTCCACAGTAAGGTGAACAATTAAGATGAGATTACCAACTGAGATTACCCATTTCAGAAAGAAGTATTCTAAGTAAAAGGTAATCAAAAGTTGGCTTTTCTACTTTCAATAGAGGATTCACACATAGTCATTGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1112:31421:19304145Test_seq23697660151M=236629-498TAAAATACATGCAGCAGAAGGTTAGTACGTAAAGACTAATTCAAACTATGCACTGATGTGTTGTTGTTAGCTGCTGTCAGGTTGGCCCTGACTCATGGTGATGCCACGCACAATGGAACAAAAGGCTGCCAGGTCCTGTGTTATATCCCATFFF:::FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:28Yt:Z:UU 60 60 AAGAAAAATAAAAGTAAATTCCACAGTAAGGTGAACAATTAAGATGAGATTACCAACTGAGATTACCCATTTCAGAAAGAAGTATTCTAAGTAAAAGGTAATCAAAAGTTGGCTTTTCTACTTTCAATAGAGGATTCACACATAGTCATTG TAAAATACATGCAGCAGAAGGTTAGTACGTAAAGACTAATTCAAACTATGCACTGATGTGTTGTTGTTAGCTGCTGTCAGGTTGGCCCTGACTCATGGTGATGCCACGCACAATGGAACAAAAGGCTGCCAGGTCCTGTGTTATATCCCAT +A00126:224:H7HMWDSX3:3:1112:21314:19586 Test_seq 166044 Test_seq 166519 + - UU A00126:224:H7HMWDSX3:3:1112:21314:19586161Test_seq16604460151M=166369476TGAGAATTTGGGGTCTGTATCCCATTGGTCTCCTGCTCCCTCAGGAGTTGTCTGTTGTGCTCCCTGACAGGGCAGACATCGATTGTGGCCGGGTACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FNM:i:1MD:Z:93C57MC:Z:151MAS:i:146XS:i:76Yt:Z:UU A00126:224:H7HMWDSX3:3:1112:21314:1958681Test_seq16636960151M=166044-476CATTGACTTAGAAGTCCCCTCAAACCATGTTCCCCAGACCCCAGCCCCTGCTCCGCTGACCTTTGAAGCATTCATTTTATCCCGGAAACCTCTTTGCTTTTAGTCCAGTCCAGTTAGGCTGACCTTCCTTGTATTGAGTGTTGTCTTTCCCFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFNM:i:5MD:Z:1G14T52T42A23T14MC:Z:151MAS:i:129XS:i:30Yt:Z:UU 60 60 TGAGAATTTGGGGTCTGTATCCCATTGGTCTCCTGCTCCCTCAGGAGTTGTCTGTTGTGCTCCCTGACAGGGCAGACATCGATTGTGGCCGGGTACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTT CATTGACTTAGAAGTCCCCTCAAACCATGTTCCCCAGACCCCAGCCCCTGCTCCGCTGACCTTTGAAGCATTCATTTTATCCCGGAAACCTCTTTGCTTTTAGTCCAGTCCAGTTAGGCTGACCTTCCTTGTATTGAGTGTTGTCTTTCCC +A00126:224:H7HMWDSX3:3:1112:25563:19680 Test_seq 95887 Test_seq 95988 + - UU A00126:224:H7HMWDSX3:3:1112:25563:1968097Test_seq9588738102M=95891102TACTTGTGGTCCATTAGTCCTTTGGACTAATCTTTCCCTTGTGTCTTTGGTTTTCTTCATTTTCCCTTACTCCAGACGAGGTGAGACCAGTGGAGTATCTTAFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:F::FFFFFF:FFFFFFFFFFFFFFFFFFFFFF:F:FFFFF:FFFFFFFFF,FF:FFFFFFFFFFFFNM:i:0MD:Z:102MC:Z:4S98MAS:i:102XS:i:87XA:Z:Test_seq,+81335,102M,3;Yt:Z:UU A00126:224:H7HMWDSX3:3:1112:25563:19680145Test_seq9589114S98M=95887-102GTGGTGTGGTACATTAGTCCTTTGGACTAATCTTTCCCTTGTGTCTTTGGTTTTCTTCATTCTGCCTTGCTCCAGATGGGGTGAGACCAGTGGAGTATCTTA:,,:F:FFFF,FF:F:FFFF,FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF,FFF,FFF:FF,FFFF:FFFF,F,FFFFFFFFFFFFFFFFFFFFFFFNM:i:6MD:Z:6C50T1C4A7C1A23MC:Z:102MAS:i:68XS:i:66XA:Z:Test_seq,-81346,11S91M,5;Yt:Z:UU 38 1 TACTTGTGGTCCATTAGTCCTTTGGACTAATCTTTCCCTTGTGTCTTTGGTTTTCTTCATTTTCCCTTACTCCAGACGAGGTGAGACCAGTGGAGTATCTTA GTGGTGTGGTACATTAGTCCTTTGGACTAATCTTTCCCTTGTGTCTTTGGTTTTCTTCATTCTGCCTTGCTCCAGATGGGGTGAGACCAGTGGAGTATCTTA +A00126:224:H7HMWDSX3:3:1112:14479:19899 Test_seq 166210 Test_seq 166566 + - UU A00126:224:H7HMWDSX3:3:1112:14479:1989997Test_seq16621060151M=166417357TTAGTTGTCGTGTGGCCTTGGTGTTCTTCCTTTGCCTTTGCTCCAGGTGGGTTGAGACCAATTGATGTATCTTAGATGGCCGCTTGTTGGCATTTAGGACCCCAGGTGCCACAATTCAAAGTGGGATGCAGAATGTTTTCATAATAGAATTFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFF:FFFF,:FFFFFFFFFFFFFFF:FFF,FFF:FFFFFF:FFFFFFFFFFFFFF:FNM:i:0MD:Z:151MC:Z:140M1I10MAS:i:151XS:i:33Yt:Z:UU A00126:224:H7HMWDSX3:3:1112:14479:19899145Test_seq16641760140M1I10M=166210-357TGCTCCGCTGACCTTTGAAGCTTTCATTTTATCCTGGAAACCTCTTTGCTTTTAGTCCAGTCCAATTAGGCTGACCTTCCTTGTATTGTGTGTTGTCTTTCCCTTCACCCAAAGCAGTTCTTATCTACTGATTGATAAATAAAAAAACCCTFFFFFFFFFFFFF,F,FFFFFFFFFFFFFFFFFFFFFFFFFFFF,:FFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:3MD:Z:34C101C13MC:Z:151MAS:i:136XS:i:30Yt:Z:UU 60 60 TTAGTTGTCGTGTGGCCTTGGTGTTCTTCCTTTGCCTTTGCTCCAGGTGGGTTGAGACCAATTGATGTATCTTAGATGGCCGCTTGTTGGCATTTAGGACCCCAGGTGCCACAATTCAAAGTGGGATGCAGAATGTTTTCATAATAGAATT TGCTCCGCTGACCTTTGAAGCTTTCATTTTATCCTGGAAACCTCTTTGCTTTTAGTCCAGTCCAATTAGGCTGACCTTCCTTGTATTGTGTGTTGTCTTTCCCTTCACCCAAAGCAGTTCTTATCTACTGATTGATAAATAAAAAAACCCT +A00126:224:H7HMWDSX3:3:1112:31213:20290 Test_seq 171742 Test_seq 172147 + - UU A00126:224:H7HMWDSX3:3:1112:31213:20290161Test_seq17174260151M=172001406CTTGGCTTTTTCTGCATTTATCCTAATTTCATTTGTGATATCTTTAAGCATTCTGTAAATTAGTTTTTTATATTCTGTATCTGATAATTCCAGGATTGTATCTTCATTTGGGAAAGATTTTGATTCTTTTGTTTGGGGGGTTGGAGAAGCTF,FFFFFF:FFFFFFFFFF:FFFF::FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFF:FFFFF:FFFFF,FFFFFFFFFFFFFFFFFF,::FFF:F:FFFFFFFFFFFFF,FFNM:i:1MD:Z:16G134MC:Z:127M4I20MAS:i:146XS:i:25Yt:Z:UU A00126:224:H7HMWDSX3:3:1112:31213:2029081Test_seq17200160127M4I20M=171742-406AAAACGGTCGCTGTCTGCCCGTGTTTGTTCGTTTTCCGTCTCTAAGTCTGTGCTTGTTGTTCAGAGTTCGTAGATTGTTATGTATGTGATCGATTCACTTGTTTTTCCGAGTCTTTGTTGCACGAGGGATCGATCCGCGGTAGCGTCCACCFF::FFFFFFF,FFF:FFFFFFFF,FFFFFF::,FFFFFFFF,F:FFF,F:FFFFFFFFF:F:FFFFF:FF,FFFFFFFF,FF,,F,F::FFFF,FFFFFFFF:FFFF,FFFFF:,F:FFFF,FFFFFFFFFFFFFFF:F,FFFFFFF:FFNM:i:13MD:Z:4T1A5A9A10C2T15T28A40A24MC:Z:151MAS:i:95XS:i:0Yt:Z:UU 60 60 CTTGGCTTTTTCTGCATTTATCCTAATTTCATTTGTGATATCTTTAAGCATTCTGTAAATTAGTTTTTTATATTCTGTATCTGATAATTCCAGGATTGTATCTTCATTTGGGAAAGATTTTGATTCTTTTGTTTGGGGGGTTGGAGAAGCT AAAACGGTCGCTGTCTGCCCGTGTTTGTTCGTTTTCCGTCTCTAAGTCTGTGCTTGTTGTTCAGAGTTCGTAGATTGTTATGTATGTGATCGATTCACTTGTTTTTCCGAGTCTTTGTTGCACGAGGGATCGATCCGCGGTAGCGTCCACC +A00126:224:H7HMWDSX3:3:1112:3341:20932 Test_seq 166640 Test_seq 214068 + + UU A00126:224:H7HMWDSX3:3:1112:3341:20932129Test_seq166640215S136M=21406847429AAATAAAACAGGATCGATCTTATAATAGTGGTCTTATACAATATTTGTCCATTTGCCTCTGACTAATTTCGCTCAGCATAATGCCTTCCAGGTTCCTCCATGTTATGAAATGTTTCATGGATTCGTCACTGTTCTTTATCGATGCTTAGTAFFFFFFFFFFF:FFFFFF:FFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFF:F,FFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFNM:i:8MD:Z:35T13C5A12T7A25G0A26G5MC:Z:151MAS:i:96XS:i:93XA:Z:Test_seq,-214259,126M1I9M15S,8;Test_seq,+400061,15S102M34S,5;Yt:Z:UU A00126:224:H7HMWDSX3:3:1112:3341:2093265Test_seq21406860151M=166640-47429AACTACCATACAACCCAGAAATCCCACTCCTCGGAATATACCCTAGAGAAATAAGAGCCTTCACACAAACAGATATATGCACACCCATGTTTATTGCAGCTCTGTTTACAATAGCAAAAAGCTGGAAGCAACCAAGGTGTCCATCAATGGAFFFFFFFFFFFFFFF:FFFFFFF:FFFFF:FFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFF:F:FFFFFFF:FFFFFFFFFFFFFFF:F:FFFNM:i:0MD:Z:151MC:Z:15S136MAS:i:151XS:i:72Yt:Z:UU 2 60 AAATAAAACAGGATCGATCTTATAATAGTGGTCTTATACAATATTTGTCCATTTGCCTCTGACTAATTTCGCTCAGCATAATGCCTTCCAGGTTCCTCCATGTTATGAAATGTTTCATGGATTCGTCACTGTTCTTTATCGATGCTTAGTA AACTACCATACAACCCAGAAATCCCACTCCTCGGAATATACCCTAGAGAAATAAGAGCCTTCACACAAACAGATATATGCACACCCATGTTTATTGCAGCTCTGTTTACAATAGCAAAAAGCTGGAAGCAACCAAGGTGTCCATCAATGGA +A00126:224:H7HMWDSX3:3:1112:28664:21418 ! 0 Test_seq 165785 - - NR A00126:224:H7HMWDSX3:3:1112:28664:2141897Test_seq1653516023S128M=165635435ACGCTGCCCGGCCAAATCCGATCAATCCACCAGGTGCTCCTTGGAAACCCTATGGGGCAGTTCTACTCTGTCCTATAGGGTTGTTATGAGTTGAAATCGACTTGATGGCAATGGGGTTTTTTACGGTGGCTTTGTTTTTTTTTTTTTTTTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF,,FFFFFFFFFFFFFFFFFFFFFFF,:FFFFFFFFFFFFFFFFFFF,,,F:FFF:F,FFFFFFFFF:NM:i:0MD:Z:128MC:Z:151MAS:i:128XS:i:46Yt:Z:NR A00126:224:H7HMWDSX3:3:1112:28664:21418145Test_seq16563560151M=165351-435TTTTTTTTTTTAATTAATTTTTATTAAGCTTCAAGTGAACATTTACCATTCCAATCAGTCTGTCACATGTAGGTTTACATACATCATACTCCCTTCTCCCACTTGCTCTCGCCCTATTGAGTCAGCCCTTTCAGTCTCTCGTTTCGTGCCAFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:23S128MAS:i:151XS:i:0Yt:Z:NR 0 60 TTTTTTTTTTTAATTAATTTTTATTAAGCTTCAAGTGAACATTTACCATTCCAATCAGTCTGTCACATGTAGGTTTACATACATCATACTCCCTTCTCCCACTTGCTCTCGCCCTATTGAGTCAGCCCTTTCAGTCTCTCGTTTCGTGCCA +A00126:224:H7HMWDSX3:3:1112:10158:21809 Test_seq 169288 Test_seq 169678 + - UU A00126:224:H7HMWDSX3:3:1112:10158:2180997Test_seq16928860151M=169528391GTTATTTGTTTCCTTTCCTGTATTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTAFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF:FFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFNM:i:1MD:Z:21T129MC:Z:151MAS:i:146XS:i:106Yt:Z:UU A00126:224:H7HMWDSX3:3:1112:10158:21809145Test_seq16952860151M=169288-391CTTTCTTCTTTTTGTATGTGTGCATTTATTGATATAAATTGGCCTCTGAGCACTGCTTTTGCTGTGTCCCAGAGGTTTTGATAGGAAGTATTTTCATTCTCGTTGCTTTCTATGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCFFFFF:FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:4MD:Z:29C70T11A22G15MC:Z:151MAS:i:131XS:i:88Yt:Z:UU 60 60 GTTATTTGTTTCCTTTCCTGTATTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTA CTTTCTTCTTTTTGTATGTGTGCATTTATTGATATAAATTGGCCTCTGAGCACTGCTTTTGCTGTGTCCCAGAGGTTTTGATAGGAAGTATTTTCATTCTCGTTGCTTTCTATGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCC +A00126:224:H7HMWDSX3:3:1112:9896:22106 ! 0 Test_seq 170435 - - NR A00126:224:H7HMWDSX3:3:1112:9896:2210697Test_seq1701446023S128M=170285292TTTCTGTCAATGGAAATATATTAGATCAATTGTCCCTTTTGTCATTATATAGTGTCCTTCTTTATCCTTTGTGGTGGATTTAAGTCTAAAGTCTATTTTGTCAGAAATTAATATTGGTCCTCCTCTTCTTTTTTGCTTATTGTTTGCTTGAFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFFFFFFFFFFF::F:FFFFF:FF,FFFFFFF:FF:FFFFFFFFFFFFFFFF,FFFF:FFFFF,F,,FF::,::FF,:FFFF::FF:FFF:FF:::F:NM:i:6MD:Z:17A33C0A40C1A22A9MC:Z:151MAS:i:98XS:i:57Yt:Z:NR A00126:224:H7HMWDSX3:3:1112:9896:22106145Test_seq17028560151M=170144-292ATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGGGTAATTATAGAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:139C11MC:Z:23S128MAS:i:146XS:i:53Yt:Z:NR 0 60 ATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGGGTAATTATAGA +A00126:224:H7HMWDSX3:3:1112:14760:23954 Test_seq 168773 Test_seq 169117 + - UU A00126:224:H7HMWDSX3:3:1112:14760:2395497Test_seq1687736015S15M1D121M=168967345TATACACCACAGTTAGATCAGGGTGAATTATTTTTTGGTGTATTGTTGGATTCTATTGGCTAGAATTGTGTTGAGGATTTTTGCATCTATGTTCATGAAGGATATAGGTCTAAAATTTTCTTTTTTTGTAATGTCTTTACCTGGTTTTGGTF:F,FFFF,FFFFF:FFFFFFFF:FFF,FFF:::::FF:FF:,FFFFFFFFFFF,FFFFF::FFF,:,:FFFFFFFFFF,FFFFFFFFFFF,FFF:FF:FFFFFF,FFFFFFF:FFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFF,FFFFNM:i:5MD:Z:15^T7A3G25T30G52MC:Z:151MAS:i:109XS:i:62Yt:Z:UU A00126:224:H7HMWDSX3:3:1112:14760:23954145Test_seq16896760151M=168773-345CTTTGAAATACCTTCAGTAGTAGTGGTGTTAAGTCTTCTCTGAAGGTTTGGTAGAACTCTGCAATGAAGCCGTCTGGGCCAGGACTTTTTTTTGTTGGAAGTTTTTTGATTACCGTTTCAATCTCTTTTTTTGTTATGGGTCTATTTAGTTFFFFFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFF:F::FFFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:NM:i:1MD:Z:63G87MC:Z:15S15M1D121MAS:i:146XS:i:67Yt:Z:UU 60 60 TATACACCACAGTTAGATCAGGGTGAATTATTTTTTGGTGTATTGTTGGATTCTATTGGCTAGAATTGTGTTGAGGATTTTTGCATCTATGTTCATGAAGGATATAGGTCTAAAATTTTCTTTTTTTGTAATGTCTTTACCTGGTTTTGGT CTTTGAAATACCTTCAGTAGTAGTGGTGTTAAGTCTTCTCTGAAGGTTTGGTAGAACTCTGCAATGAAGCCGTCTGGGCCAGGACTTTTTTTTGTTGGAAGTTTTTTGATTACCGTTTCAATCTCTTTTTTTGTTATGGGTCTATTTAGTT +A00126:224:H7HMWDSX3:3:1112:2302:24424 Test_seq 168616 Test_seq 212800 - - UU A00126:224:H7HMWDSX3:3:1112:2302:24424113Test_seq16846660151M=21266844185GGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTFFFFFFFFFFF::FFFFFFFFFFF:FFFF,FF:,F:FF::FFFFF,::FFFF,FFFF:FFF:::FFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFF,:FFFFFFFFFFFF,:FFFFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFFNM:i:1MD:Z:29T121MC:Z:133M18SAS:i:146XS:i:91Yt:Z:UU A00126:224:H7HMWDSX3:3:1112:2302:24424177Test_seq21266827133M18S=168466-44185TATCACTATTTGCATATGACATGATTATATACACAGAAAACCCTAAGGAATCATCCAGAAAACTACTGAAACTAATAGAAGAGTTTGGCAGAGTCTCAGGTTATAAAATAAACATACAAAAATCACTTGGATTAGTAAAGAACAACTAGATFFFFF,FF:FFF:F,FFFF,FFFFFFFFFFFFF:FFFFFFF,,FFF,,FFF:,FFFFFFFFF:FFFF:FFFF:F:,F:FFFFFFFFF:FF:FFF,,F,F:F:FFFFFFFFFFFFFF:FFFFFF::,FFF,FFFFFF,,FFFF,:F,:::FFNM:i:7MD:Z:4T9G10C0T17T7C53G26MC:Z:151MAS:i:98XS:i:83XA:Z:Test_seq,+168238,18S133M,10;Yt:Z:UU 60 27 GGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTT TATCACTATTTGCATATGACATGATTATATACACAGAAAACCCTAAGGAATCATCCAGAAAACTACTGAAACTAATAGAAGAGTTTGGCAGAGTCTCAGGTTATAAAATAAACATACAAAAATCACTTGGATTAGTAAAGAACAACTAGAT +A00126:224:H7HMWDSX3:3:1112:28592:24580 Test_seq 169132 Test_seq 169431 + - UU A00126:224:H7HMWDSX3:3:1112:28592:2458097Test_seq16913254151M=169281300TGTGTTAGTTTAGGTAGGTAGTGTTTTTCCAAGAATTTATCCATTTCTTCTAAGTTTTCAAATTAGTTAGAGTACAATTTTTCGTAGTAATCTGAAATGATTCTTTTAATTTCATTTGGTTCTGTTTTGATGTGGTCCTTCTCGTTTCTTAFFFFFFFFFFF,:FFFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::F:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFNM:i:9MD:Z:29G7C14G11T4C11A37C6G9T14MC:Z:151MAS:i:106XS:i:74Yt:Z:UU A00126:224:H7HMWDSX3:3:1112:28592:24580145Test_seq16928160151M=169132-300TATTCGGGTTATTTGTTTCCTTTCCTGTATTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAFF:FFFFF:FFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:28T122MC:Z:151MAS:i:146XS:i:103Yt:Z:UU 54 60 TGTGTTAGTTTAGGTAGGTAGTGTTTTTCCAAGAATTTATCCATTTCTTCTAAGTTTTCAAATTAGTTAGAGTACAATTTTTCGTAGTAATCTGAAATGATTCTTTTAATTTCATTTGGTTCTGTTTTGATGTGGTCCTTCTCGTTTCTTA TATTCGGGTTATTTGTTTCCTTTCCTGTATTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTA +A00126:224:H7HMWDSX3:3:1112:30861:24721 Test_seq 341770 Test_seq 342069 + - UU A00126:224:H7HMWDSX3:3:1112:30861:24721161Test_seq34177060151M=341935300GCTGACAGGCTGTCCCTCCCAGGGTTGGGGTTTCCTGTTTGAAAGTGTTTAGTGTGTGCAGCCCGCTTCCAGCGTCTACTCATCTACCTTTCTGATGTAGAAGAGGGAGGGCGCCGGCAGCCTTTTTATGTCGGTAGACTTGCCCGGGGCT:FFFFF:FFFF:FFF:F:FFF:FFF:FFFFFFFFFF:F,FFFF:FF:F:FFFFFFF:FFFFFFFFFFFFFFFFF:FF:FFFF:FFFFF,FF::FF,FFFFFF:FF,FFFFFFFFFFFFFFFFF:FFFFFFFFFF,FFFF:FFFFFFFFFFFNM:i:0MD:Z:151MC:Z:135M16SAS:i:151XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1112:30861:2472181Test_seq34193560135M16S=341770-300CTGGCCCTGCCCACATCCCTAGAAGTCTTCCTTCTGCAACTCTGAAGTGGCTCTAGAGTTCTCCAATGCCCAGATAGGCAGAGCCAGACGCAGCTGGGAGTTGGAGCTAGGAGAAGAGGCAGACAAACGAGGAGTAGTCCTTAAATTCTATFFFF:FFFFF::F:FFF::FFFF,FFFFFFF:FF:::FFF,FFFF,FFFF,F,F,FF:F::FFFFFF::F:,:FF:,FF:FFFFF::F:::F:FF,F:F:FF,:FF:F::FFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFF::FF:FFNM:i:1MD:Z:40A94MC:Z:151MAS:i:130XS:i:0Yt:Z:UU 60 60 GCTGACAGGCTGTCCCTCCCAGGGTTGGGGTTTCCTGTTTGAAAGTGTTTAGTGTGTGCAGCCCGCTTCCAGCGTCTACTCATCTACCTTTCTGATGTAGAAGAGGGAGGGCGCCGGCAGCCTTTTTATGTCGGTAGACTTGCCCGGGGCT CTGGCCCTGCCCACATCCCTAGAAGTCTTCCTTCTGCAACTCTGAAGTGGCTCTAGAGTTCTCCAATGCCCAGATAGGCAGAGCCAGACGCAGCTGGGAGTTGGAGCTAGGAGAAGAGGCAGACAAACGAGGAGTAGTCCTTAAATTCTAT +A00126:224:H7HMWDSX3:3:1112:30120:25598 Test_seq 170533 Test_seq 170929 + - UU A00126:224:H7HMWDSX3:3:1112:30120:25598161Test_seq1705336061M12I78M=170779397TAGTAGCTTGTGAGATCCTCATTTTCATAATGTTTAACTTTATGTTAGTTGAGTCGTTACGTTTTTCTTGGCTTTTTTCTTGAGTTATTGAATTGATATTCCTTTTTGTGGTTAACTTATTATCTACCCCTAATTTTCTAAGTAAAAACCTFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF:F,F,FFFF:,F,:FF:FFFF,FFFF,FF:F,::F:FFFFF:FFF,FFFF:F,FFFF,F:FFFFFF:F,:FFF:F,FFFF:FFF,FFFFFF,:,NM:i:18MD:Z:6A39T29G25C8T8T18MC:Z:151MAS:i:91XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1112:30120:2559881Test_seq17077960151M=170533-397CCATGATTTCCTGTTATGTGTATTATTTTGTTTATTTATTTATTTTTTAGAATTAATCTTAATTTGTTTGTTTTTGTGCTTTCCCTATTTGAGTTGCGTTGATATCAGGACGTTCTGTTTTGTGACCTTGTATTGTGCTGGTACCTGATATFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFNM:i:1MD:Z:61C89MC:Z:61M12I78MAS:i:146XS:i:0Yt:Z:UU 60 60 TAGTAGCTTGTGAGATCCTCATTTTCATAATGTTTAACTTTATGTTAGTTGAGTCGTTACGTTTTTCTTGGCTTTTTTCTTGAGTTATTGAATTGATATTCCTTTTTGTGGTTAACTTATTATCTACCCCTAATTTTCTAAGTAAAAACCT CCATGATTTCCTGTTATGTGTATTATTTTGTTTATTTATTTATTTTTTAGAATTAATCTTAATTTGTTTGTTTTTGTGCTTTCCCTATTTGAGTTGCGTTGATATCAGGACGTTCTGTTTTGTGACCTTGTATTGTGCTGGTACCTGATAT +A00126:224:H7HMWDSX3:3:1112:14570:25755 ! 0 Test_seq 40993 - + MU A00126:224:H7HMWDSX3:3:1112:14570:25755129Test_seq167626089M=40993-126634TCGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATCATTTCCCCAATTGACTGACACTGGTCCTTTGTCAAATATFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFF,FFFFFFFFFFF,FFFFFFFFFFFFFFNM:i:1MD:Z:1T87MC:Z:89MAS:i:87XS:i:87XA:Z:Test_seq,-213324,89M,1;Test_seq,-40993,89M,3;Yt:Z:MU A00126:224:H7HMWDSX3:3:1112:14570:2575565Test_seq40993289M=167626126634ATATTTGACAAAGGCCCAGTGTCAGTTAATTGGGGAAAAGATAGTCTTTTTAACAAATGGTGCTGGCATAACTGCATATCCACTTGCAAFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF,FFFF,FNM:i:5MD:Z:14A23T35G7T0C5MC:Z:89MAS:i:67XS:i:64XA:Z:Test_seq,+213324,89M,5;Test_seq,-167626,89M,5;Yt:Z:MU 0 2 TCGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATCATTTCCCCAATTGACTGACACTGGTCCTTTGTCAAATAT ATATTTGACAAAGGCCCAGTGTCAGTTAATTGGGGAAAAGATAGTCTTTTTAACAAATGGTGCTGGCATAACTGCATATCCACTTGCAA +A00126:224:H7HMWDSX3:3:1112:5891:26256 Test_seq 214069 Test_seq 404397 + + RU A00126:224:H7HMWDSX3:3:1112:5891:26256129Test_seq21406960151M=404397190329ACTACCATACAACCCAGAAATCCCACTCCTCGGAATATACCCTAGAGAAATAAGAGCCTTCACACAAACAGATATATGCACACCCATGTTTATTGCAGCTCTGTTTACAATAGCAAAAAGCTGGAAGCAACCAAGGTGTCCATCAACGGATFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:146T4MC:Z:31M120SAS:i:146XS:i:103Yt:Z:RU A00126:224:H7HMWDSX3:3:1112:5891:2625665Test_seq4043972131M120S=214069-190329TTCTGCTCTGTCCTATAGGGTCACTATGAGTGATCTTATAATAGTGGTCTTATACAATATTTGTCCTTTTGCCTCTGACTAATTTCACTCAGCATAATGCCTTCCAGGTTCCTCCATGTTATGAAATGTTTCACAGATTCGTCACTGTTCTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:31MC:Z:151MAS:i:31XS:i:26SA:Z:Test_seq,214275,-,110M1I9M31S,12,2;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1112:5891:262562129Test_seq21427512110M1I9M31H=214069-325AGAACAGTGACGAATCTGTGAAACATTTCATAACATGGAGGAACCTGGAAGGCATTATGCTGAGTGAAATTAGTCAGAGGCAAAAGGACAAATATTGTATAAGACCACTATTATAAGATCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:2MD:Z:94C24MC:Z:151MAS:i:107XS:i:100SA:Z:Test_seq,404397,+,31M120S,21,0;XA:Z:Test_seq,+166640,31S120M,4;Test_seq,+400061,31S120M,5;Test_seq,+48610,36S115M,5;Yt:Z:RU 60 21 ACTACCATACAACCCAGAAATCCCACTCCTCGGAATATACCCTAGAGAAATAAGAGCCTTCACACAAACAGATATATGCACACCCATGTTTATTGCAGCTCTGTTTACAATAGCAAAAAGCTGGAAGCAACCAAGGTGTCCATCAACGGAT TTCTGCTCTGTCCTATAGGGTCACTATGAGTGATCTTATAATAGTGGTCTTATACAATATTTGTCCTTTTGCCTCTGACTAATTTCACTCAGCATAATGCCTTCCAGGTTCCTCCATGTTATGAAATGTTTCACAGATTCGTCACTGTTCT +A00126:224:H7HMWDSX3:3:1112:32380:26944 Test_seq 9121 Test_seq 9439 + - UU A00126:224:H7HMWDSX3:3:1112:32380:26944161Test_seq912160151M=9289319GATCCATCCGACATCAGCAAAGATATCCCGTGTTCCACCCCTTCTTCAGAATCTGGCTTGAATTCCTGGCAGTTCCCTGTCAAAATACTGCTGCAGCCACTTTTAAATGACCTTCAGCAAAATTTTACTTGGCAGTGGGTTATTTTATATTFFFFFFF::F:FF:F:FF,FFFFFFFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFFF:FFFFFFFF:FFFFFFFFF:FFFFFFFFFFFFFF:FFFFFFFFFFF,,F:,FFFF:FFFFFF,FFF:FF:FFFFFFFF,FFFFF,FFFF,FF,FNM:i:1MD:Z:141T9MC:Z:151MAS:i:146XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1112:32380:2694481Test_seq928960151M=9121-319TCTGCACTCGGTTGTACCACCTTTCTTGGAAATAGGCATACATATGGATCTCTTCCAGTCAGTTGGCTAGGTAGCTGACTTCCAAATGTCTTGGCACAGACCGGTGAGCACTTTCAGTGCTGCATCTATTTTTCTCAATTGGTATTCTCTCFF,::FFFF:F,F:FFFF::FF:FFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFF,:F:FF:F:FFFFF:F:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:F:FFFF:,FFFF,FFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU 60 60 GATCCATCCGACATCAGCAAAGATATCCCGTGTTCCACCCCTTCTTCAGAATCTGGCTTGAATTCCTGGCAGTTCCCTGTCAAAATACTGCTGCAGCCACTTTTAAATGACCTTCAGCAAAATTTTACTTGGCAGTGGGTTATTTTATATT TCTGCACTCGGTTGTACCACCTTTCTTGGAAATAGGCATACATATGGATCTCTTCCAGTCAGTTGGCTAGGTAGCTGACTTCCAAATGTCTTGGCACAGACCGGTGAGCACTTTCAGTGCTGCATCTATTTTTCTCAATTGGTATTCTCTC +A00126:224:H7HMWDSX3:3:1112:25012:28494 ! 0 Test_seq 339682 - + NR A00126:224:H7HMWDSX3:3:1112:25012:2849481Test_seq33992460126M25S=339682-368CACCCTTTGGAGGACATTCACATCAGCATTTATTTTAAACTGTTACAGCAACTCAGAAAAACAGGTTGGAAAATACAGAAACTTTCCATGAAAAATGACTAAAAGGAAGTCAATGTAAGAGTTTTATAAGAAAATAAAATTCAAACCGCAGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:126MC:Z:151MAS:i:126XS:i:0Yt:Z:NR A00126:224:H7HMWDSX3:3:1112:25012:28494161Test_seq33968260151M=339924368TCAGAGCTTGGAACCAAAAACCAAACCCACTGGCTTCCAGTCGATTCTGACTCATAGCGACCCTATAGGACAGAGTAGAACTGCCCCATAGAGTTTCCAAGGAGCGCCTGGTGGATTTGAACTGCCAACCTCTTGGTTAGCAGCCATAGCAFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFF,FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:126M25SAS:i:151XS:i:89Yt:Z:NR 0 60 TCAGAGCTTGGAACCAAAAACCAAACCCACTGGCTTCCAGTCGATTCTGACTCATAGCGACCCTATAGGACAGAGTAGAACTGCCCCATAGAGTTTCCAAGGAGCGCCTGGTGGATTTGAACTGCCAACCTCTTGGTTAGCAGCCATAGCA +A00126:224:H7HMWDSX3:3:1112:27941:31688 Test_seq 167567 Test_seq 213182 + + UU A00126:224:H7HMWDSX3:3:1112:27941:31688129Test_seq1675672619S132M=21318245616GAGGCTGGGCGGTGGGGAGTGATCCATTTGGAGTTAGTTTTTGTGCATAGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATCATTTCCCCAATTGACTGACACTGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFNM:i:2MD:Z:7C21G102MC:Z:151MAS:i:122XS:i:110XA:Z:Test_seq,-213340,120M31S,2;Yt:Z:UU A00126:224:H7HMWDSX3:3:1112:27941:3168865Test_seq2131827151M=167567-45616AGGCCTCACTCTACCTGATTTCAGAACCTATTATACAGCTACAGTAGTCAAAACAGCCTGGTACTGGTACAACAACAGGCACATAGACCAATGGAACAGAATTGAGAACCCAGATATAAATCCATCCATGTATGAGCAGCTGATATTTGACFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:130A20MC:Z:19S132MAS:i:146XS:i:141XA:Z:Test_seq,-167706,151M,2;Yt:Z:UU 26 7 GAGGCTGGGCGGTGGGGAGTGATCCATTTGGAGTTAGTTTTTGTGCATAGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATCATTTCCCCAATTGACTGACACTG AGGCCTCACTCTACCTGATTTCAGAACCTATTATACAGCTACAGTAGTCAAAACAGCCTGGTACTGGTACAACAACAGGCACATAGACCAATGGAACAGAATTGAGAACCCAGATATAAATCCATCCATGTATGAGCAGCTGATATTTGAC +A00126:224:H7HMWDSX3:3:1112:19931:32690 Test_seq 168171 Test_seq 168621 + - UU A00126:224:H7HMWDSX3:3:1112:19931:3269097Test_seq1681717151M=168471451GGGGGCTACTGTGAATGGTATTGATTTAGTTATTTCCTCTTCGGTGTTCTTTTTGTTGATGTAGAGGAATCCAAGTGATTTTTGTATGTTTATTTTATAACCTGAGACTCTGCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF,FFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:3MD:Z:27G72T10T39MC:Z:151MAS:i:136XS:i:131XA:Z:Test_seq,-212717,151M,4;Yt:Z:UU A00126:224:H7HMWDSX3:3:1112:19931:32690145Test_seq16847160151M=168171-451TAAAGGGCATCCTTGTCTGGTTCCTGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:91Yt:Z:UU 7 60 GGGGGCTACTGTGAATGGTATTGATTTAGTTATTTCCTCTTCGGTGTTCTTTTTGTTGATGTAGAGGAATCCAAGTGATTTTTGTATGTTTATTTTATAACCTGAGACTCTGCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGAT TAAAGGGCATCCTTGTCTGGTTCCTGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATC +A00126:224:H7HMWDSX3:3:1112:24551:34053 Test_seq 200001 Test_seq 200496 + - UU A00126:224:H7HMWDSX3:3:1112:24551:3405397Test_seq20000160151M=200346496GATCAAATGTTTTTGGAAACATTTTCATTATGAGCAGAGGTTTTTTTTGTTTGTTTTTGTTTTTTAATTTCAGCAATCCAGGGTTCAGATTGCTCTACTATTTGCTAACTTTAGGAAGTGTAAAAAGCATATTTTTCTTACCTGAAAAGGAFFFFF:::FFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF,FFFFFF::FF:FFFFF:::FFFFFFFFFF:FFF:FFFFFFF::FFFFF,FFFFFF,FFFFFFFF:FFFFFFFF:,:F,F:,F:FNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1112:24551:34053145Test_seq20034660151M=200001-496TTTGAACTGCCAGTCTTTTGGTTAGCAGCTGTAGCACTTAACTACTACGCCACCAGGGTTACCACTCAGCACAGAGGAAAAGCTTAATAAATGGTAACTATCATAATCGTCAAGGAGCCCTGGTGGCGTGGTGGTTAAAGCACTTGGCTGTFF:FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,:FFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU 60 60 GATCAAATGTTTTTGGAAACATTTTCATTATGAGCAGAGGTTTTTTTTGTTTGTTTTTGTTTTTTAATTTCAGCAATCCAGGGTTCAGATTGCTCTACTATTTGCTAACTTTAGGAAGTGTAAAAAGCATATTTTTCTTACCTGAAAAGGA TTTGAACTGCCAGTCTTTTGGTTAGCAGCTGTAGCACTTAACTACTACGCCACCAGGGTTACCACTCAGCACAGAGGAAAAGCTTAATAAATGGTAACTATCATAATCGTCAAGGAGCCCTGGTGGCGTGGTGGTTAAAGCACTTGGCTGT +A00126:224:H7HMWDSX3:3:1112:23285:36213 Test_seq 170144 Test_seq 170578 + - UU A00126:224:H7HMWDSX3:3:1112:23285:3621397Test_seq17014460151M=170428435GATCAATTGTCCCTTTTATCATTATGTAGTGTCCTTCTTTATCCTTTGTGGTGGATTTAAGTCTAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCCTCTTCTTTTTTGCTTATTGTTTGCTTGATATATTTTTTTCCATCCTTTGAGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFF:FFFFFFFFFF::FFF:,FF::::FFFFFFFFFFFFF,FFFFFFFFFFFNM:i:4MD:Z:25A25C0A65A32MC:Z:151MAS:i:131XS:i:59Yt:Z:UU A00126:224:H7HMWDSX3:3:1112:23285:36213145Test_seq17042860151M=170144-435ATTATAGATAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTTTTCATGTGTGTTGTTGGCCATTTCATTTTTCCACATACTTTTTTGTGCTGAGACGTTTTTCTTAGTAGATTGTGAGATCCTCATTTTCATAATGTTTAACTTTATGTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:62T88MC:Z:151MAS:i:146XS:i:25Yt:Z:UU 60 60 GATCAATTGTCCCTTTTATCATTATGTAGTGTCCTTCTTTATCCTTTGTGGTGGATTTAAGTCTAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCCTCTTCTTTTTTGCTTATTGTTTGCTTGATATATTTTTTTCCATCCTTTGAG ATTATAGATAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTTTTCATGTGTGTTGTTGGCCATTTCATTTTTCCACATACTTTTTTGTGCTGAGACGTTTTTCTTAGTAGATTGTGAGATCCTCATTTTCATAATGTTTAACTTTATGTT +A00126:224:H7HMWDSX3:3:1112:27986:36777 ! 0 Test_seq 213955 - - MU A00126:224:H7HMWDSX3:3:1112:27986:36777113Test_seq1673130151M=21380546493AGAGATCAGGCGCTGGTCGGAGATGTCATAGCTGAAAATTCTTTCCCAGTCTGTAAGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTCGTCATTTTTGFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:4MD:Z:10A7T17T104A9MC:Z:151MAS:i:131XS:i:131XA:Z:Test_seq,+213571,151M,4;Test_seq,-400734,126M25S,4;Yt:Z:MU A00126:224:H7HMWDSX3:3:1112:27986:36777177Test_seq21380544151M=167313-46493CTTCACTAAAGAAGATATTCAGGCAGCTAACAGATACATGAGAAAATGCTCTCGATCATTAGCCATTAGAGAAATGCAAATTAAAACTACGATGAGATTCCATCTCACTCCAACAAGGCTGGCATTAATCCAAAAAACACAAAATAATAAAFFFF:F:FFFFFFFFFFFFFF,FFFFFF:FFFFFFFFFFF:FFF:FFFFFF:FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:86G64MC:Z:151MAS:i:146XS:i:125XA:Z:Test_seq,+167079,151M,6;Yt:Z:MU 0 44 AGAGATCAGGCGCTGGTCGGAGATGTCATAGCTGAAAATTCTTTCCCAGTCTGTAAGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTCGTCATTTTTG CTTCACTAAAGAAGATATTCAGGCAGCTAACAGATACATGAGAAAATGCTCTCGATCATTAGCCATTAGAGAAATGCAAATTAAAACTACGATGAGATTCCATCTCACTCCAACAAGGCTGGCATTAATCCAAAAAACACAAAATAATAAA +A00126:224:H7HMWDSX3:3:1112:24695:36714 Test_seq 169001 Test_seq 169460 + - UU A00126:224:H7HMWDSX3:3:1112:24695:36714161Test_seq1690016017S134M=169310460CCAAAAGCGATCTAAGCCTTCTCTGAAGGTTTGGTAGAACTCTGCAGTGAAGTCATCTGGGCCAGGACTTTTTTTTGTTGGGAGTTTTTTGATTACCGTTTCAATCTCTTTTTGTGTTATGGGTCTATTTAGTTGTTCTACTTCTGAATGTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFF:FF:FFF:FFFFFFFFFFFFFFF,FFFFFF:FFFFFFF:FFFFFFFFFF:FFFFF:FF:FFFFF,FFFFFF,FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF:NM:i:4MD:Z:35C1G26A31T37MC:Z:151MAS:i:114XS:i:60Yt:Z:UU A00126:224:H7HMWDSX3:3:1112:24695:3671481Test_seq16931060151M=169001-460TTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:17S134MAS:i:151XS:i:110Yt:Z:UU 60 60 CCAAAAGCGATCTAAGCCTTCTCTGAAGGTTTGGTAGAACTCTGCAGTGAAGTCATCTGGGCCAGGACTTTTTTTTGTTGGGAGTTTTTTGATTACCGTTTCAATCTCTTTTTGTGTTATGGGTCTATTTAGTTGTTCTACTTCTGAATGT TTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCC +A00126:224:H7HMWDSX3:3:1112:22589:36886 ! 0 Test_seq 213149 - + NR A00126:224:H7HMWDSX3:3:1112:22589:36886129Test_seq1675324228S123M=21314945618CCTTTGGTGTTGGTTTTGTTTTTTGATCGATCTTTATCATTTTAGTCTTTATGTTTAGGTCTTTGATCCACCTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATCCTTTTGCAAATGGATATCCAGTTATGCCAGCAFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:FFFF:FF::F::FFFFFF,F,FF:FNM:i:3MD:Z:10G32T46T32MC:Z:151MAS:i:108XS:i:88XA:Z:Test_seq,+400995,28S118M5S,6;Yt:Z:NR A00126:224:H7HMWDSX3:3:1112:22589:3688665Test_seq2131496151M=167532-45618CAAAGCATTACTGAAAAAGAAGAAGAAAGTGGGAGGCCTCACTCTACCTGATTTCAGAACCTATTATACAGCTACAGTAGTCAAAACAGCCTGGTACTGGTACAACAACAGGCACATAGACCAATGGAACAGAATTGAGAACCCAGATATAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:0T150MC:Z:28S123MAS:i:150XS:i:146XA:Z:Test_seq,-167739,151M,1;Yt:Z:NR 0 6 CAAAGCATTACTGAAAAAGAAGAAGAAAGTGGGAGGCCTCACTCTACCTGATTTCAGAACCTATTATACAGCTACAGTAGTCAAAACAGCCTGGTACTGGTACAACAACAGGCACATAGACCAATGGAACAGAATTGAGAACCCAGATATA +A00126:224:H7HMWDSX3:3:1113:12011:1908 Test_seq 170321 Test_seq 170762 + - UU A00126:224:H7HMWDSX3:3:1113:12011:1908161Test_seq17032160151M=170612442TAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCATGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFF:FFFF,FFF:F::NM:i:1MD:Z:56G94MC:Z:151MAS:i:146XS:i:54Yt:Z:UU A00126:224:H7HMWDSX3:3:1113:12011:190881Test_seq17061260151M=170321-442ATTGATATTCCTTTTTGTGGTTACCTTATTATTTACCCCTATTTTTCTAAGTAAAAACCTAACTTGTATCGTTCTATATCGCCTTGTATCACTCTCCATCTGGCAGTTCAATGCCTCCTATGTTTAGTCCCCCTTTGTGATTATTGTGATCFFFFFFFFFFF,FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:6MD:Z:73G6A38G1A9T4T14MC:Z:151MAS:i:121XS:i:37Yt:Z:UU 60 60 TAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCATGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTTT ATTGATATTCCTTTTTGTGGTTACCTTATTATTTACCCCTATTTTTCTAAGTAAAAACCTAACTTGTATCGTTCTATATCGCCTTGTATCACTCTCCATCTGGCAGTTCAATGCCTCCTATGTTTAGTCCCCCTTTGTGATTATTGTGATC +A00126:224:H7HMWDSX3:3:1113:24659:2049 Test_seq 362109 Test_seq 362498 + - UU A00126:224:H7HMWDSX3:3:1113:24659:204997Test_seq36210960151M=362364390ATGCGCCCACGTGCACCATGGTTTCTTCAGCCATGCTTCTTACTACAGAAAGTGATTCCCAACTCTACATACTCAGGAAAGTTATTGGGTTGTCTTTTTCTGTTTAAAACTATTTGTGCTTATCGTGGACAATATTGAAAGTAATAAGAAAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:135M16SAS:i:151XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1113:24659:2049145Test_seq36236460135M16S=362109-390ATGAATACATATCAGAGATTTATTTGACTCATTAATGAGGGAACCAGTAAGATGGTAAAGCTAGTTCCAAAACGTATAGGGAAAACAGAAGTATTTTAGTTATTAAAAGAAAGTTGAAATAAAATTTCTGGATCACTCTTAAAACCCAGAGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:135MC:Z:151MAS:i:135XS:i:0Yt:Z:UU 60 60 ATGCGCCCACGTGCACCATGGTTTCTTCAGCCATGCTTCTTACTACAGAAAGTGATTCCCAACTCTACATACTCAGGAAAGTTATTGGGTTGTCTTTTTCTGTTTAAAACTATTTGTGCTTATCGTGGACAATATTGAAAGTAATAAGAAA ATGAATACATATCAGAGATTTATTTGACTCATTAATGAGGGAACCAGTAAGATGGTAAAGCTAGTTCCAAAACGTATAGGGAAAACAGAAGTATTTTAGTTATTAAAAGAAAGTTGAAATAAAATTTCTGGATCACTCTTAAAACCCAGAG +A00126:224:H7HMWDSX3:3:1113:13123:3364 Test_seq 168222 Test_seq 168674 + - UU A00126:224:H7HMWDSX3:3:1113:13123:3364161Test_seq16822243151M=168524453TTTGTTGATGTAGAGGAATCCAAGTGATTTTTGTATGTTTATTTTATAACCTGAGACTCTTCCAAACTCTTCTATTAGTTTCAGTAGCTTTCTGGAGGATTCCTTAGGGTTTTCCATGTATACGATCATGTCATCTGCAAATAGTGATAGCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFNM:i:4MD:Z:49T37T36G18A7MC:Z:151MAS:i:131XS:i:109XA:Z:Test_seq,-212673,7S144M,7;Yt:Z:UU A00126:224:H7HMWDSX3:3:1113:13123:336481Test_seq16852460151M=168222-453CTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU 43 60 TTTGTTGATGTAGAGGAATCCAAGTGATTTTTGTATGTTTATTTTATAACCTGAGACTCTTCCAAACTCTTCTATTAGTTTCAGTAGCTTTCTGGAGGATTCCTTAGGGTTTTCCATGTATACGATCATGTCATCTGCAAATAGTGATAGC CTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGAT +A00126:224:H7HMWDSX3:3:1113:4526:3975 Test_seq 168585 Test_seq 212883 - - UU A00126:224:H7HMWDSX3:3:1113:4526:3975113Test_seq16843560151M=21273344299TAGGACTCCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCTGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:7T143MC:Z:151MAS:i:146XS:i:78Yt:Z:UU A00126:224:H7HMWDSX3:3:1113:4526:3975177Test_seq21273320151M=168435-44299CTGAAACTAATAGAAGAGTTTGGCAGAGTCTCAGGTTATAAAATAAACATACAAAAATCACTTGGATTCCTCTACATCAACAAAAAGAACATCGAAGAGGAAATCACCAAATCAATACCATTCACAGTAGCCCCCAAGAAGATAAAATACTF:FF:FFFFFFF::FFFFFFF,:FFF:,F,FFF:FFFFFFFFFFFFF,FFFFFFFFFFFFFFF,:FFFF,F:FFFFFFFFFF:FF:FFFFFF:F,FFFFFFFFF:::FFFFF,FFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFF:FFFFFNM:i:3MD:Z:41G51A10A46MC:Z:151MAS:i:136XS:i:126XA:Z:Test_seq,+168155,151M,5;Yt:Z:UU 60 20 TAGGACTCCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCTGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAAT CTGAAACTAATAGAAGAGTTTGGCAGAGTCTCAGGTTATAAAATAAACATACAAAAATCACTTGGATTCCTCTACATCAACAAAAAGAACATCGAAGAGGAAATCACCAAATCAATACCATTCACAGTAGCCCCCAAGAAGATAAAATACT +A00126:224:H7HMWDSX3:3:1113:5565:7560 ! 0 Test_seq 166317 - + NR A00126:224:H7HMWDSX3:3:1113:5565:756081Test_seq16643460120M31S=166317-237AAGCTTTCATTTTATCCCGGAAACCTCTTTGCTTTTAGTCCAGTCCAATTAGGCTGACCTTCCTTGTATTGTGTGTTGTCTTTCCCTTCACCCAAAGCAGTTCTTATCTACTGATTGATCTAAAATTCAAGTCATATCTTGAAATTCACTA:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:120MC:Z:151MAS:i:120XS:i:30Yt:Z:NR A00126:224:H7HMWDSX3:3:1113:5565:7560161Test_seq16631760151M=166434237GCCACAATTCAAAGTGGGATGCAGAATGTTTTCATAATAGAATTATTTTCCCCGTTGACTTAGAAGTCTCCTCAAACCATGTTCCCCAGACCCCAGCCCCTGCTCCGCTGACCTTTGAAGCTTTCATTTTATCCCGGAAACCTCTTTGCTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFF:FFFFFF:FFFFNM:i:1MD:Z:49G101MC:Z:120M31SAS:i:146XS:i:32Yt:Z:NR 0 60 GCCACAATTCAAAGTGGGATGCAGAATGTTTTCATAATAGAATTATTTTCCCCGTTGACTTAGAAGTCTCCTCAAACCATGTTCCCCAGACCCCAGCCCCTGCTCCGCTGACCTTTGAAGCTTTCATTTTATCCCGGAAACCTCTTTGCTT +A00126:224:H7HMWDSX3:3:1113:12644:7701 Test_seq 424202 Test_seq 424540 + - UU A00126:224:H7HMWDSX3:3:1113:12644:770197Test_seq42420260151M=424407339TGGGGCAGAAGACTGCTGTTTTTCACACTTAGAGAATAAGACCTTTTCAAACTCAACATGTCCACTTGGATGTCTAATAGACATCTTAAACTTAATATATTTAAAACAATATTTGGCCCCAAAGCTGCCCTATTTTGTGTCAGCTGATGGCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFF,FFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:134M17SAS:i:151XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1113:12644:7701145Test_seq42440760134M17S=424202-339ATATCCAATCCTTCAGGAAATCCTTTAGAATATCTCCAGACTCTGACCATCTTGCGCTTCCTCTGCTGTCACCATCCTGGGTCAAGCCCATCATCTCTCACCTGAACTCCTGCTCATAATCTCCTCCCAGATCTAAACTACACAGTATCTAFF:FFFFFFFFFFFFFF:FFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF:FF:FFFFFFFFFNM:i:0MD:Z:134MC:Z:151MAS:i:134XS:i:0Yt:Z:UU 60 60 TGGGGCAGAAGACTGCTGTTTTTCACACTTAGAGAATAAGACCTTTTCAAACTCAACATGTCCACTTGGATGTCTAATAGACATCTTAAACTTAATATATTTAAAACAATATTTGGCCCCAAAGCTGCCCTATTTTGTGTCAGCTGATGGC ATATCCAATCCTTCAGGAAATCCTTTAGAATATCTCCAGACTCTGACCATCTTGCGCTTCCTCTGCTGTCACCATCCTGGGTCAAGCCCATCATCTCTCACCTGAACTCCTGCTCATAATCTCCTCCCAGATCTAAACTACACAGTATCTA +A00126:224:H7HMWDSX3:3:1113:13765:8735 Test_seq 167568 Test_seq 328153 + + RU A00126:224:H7HMWDSX3:3:1113:13765:8735129Test_seq16756832151M=328153160586GATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATCATTTCCCCAATTGACTGACACTGGTCCTTTGTCAAATATCAGCFFFFFFFFFFFFF,FFFF,FFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFF,FFFFFFF:FFFFFFFFFFFF::FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:2S25M124SAS:i:151XS:i:136XA:Z:Test_seq,-213320,140M2I9M,3;Yt:Z:RU A00126:224:H7HMWDSX3:3:1113:13765:87352129Test_seq16784960124M27H=167568-405TGAGGCCTCCCACTTTCTTCTTCTTTTTCAGTAATGCTTTGCTTATCCGGGGGTTCTTTCCCTTCCATATGAAATTAGTGATTTGTTTCTCTATCCCCTTAAAATATGACATTGGAATTTGGATFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFF,FFFFFFFF:FFFFFFFFFFFNM:i:1MD:Z:115T8MC:Z:151MAS:i:119XS:i:75SA:Z:Test_seq,328153,+,2S25M124S,19,0;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1113:13765:873565Test_seq328153192S25M124S=167568-160586AAGGAGCCCTGGTGGTGCAGTGGTTAGATCCAAATTCCAATGTCATATTTTAAGGGGATAGAGAAACAAATCACTAATTTCATATGGAAGGGAAAGAACCCCCGGATAAGCAAAGCATTACTGAAAAAGAAGAAGAAAGTGGGAGGCCTCAFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFFF,FFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFNM:i:0MD:Z:25MC:Z:151MAS:i:25XS:i:21SA:Z:Test_seq,167849,-,124M27S,60,1;XA:Z:Test_seq,+141607,26M125S,1;Test_seq,+432045,26M125S,1;Yt:Z:RU 32 19 GATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATCATTTCCCCAATTGACTGACACTGGTCCTTTGTCAAATATCAGC AAGGAGCCCTGGTGGTGCAGTGGTTAGATCCAAATTCCAATGTCATATTTTAAGGGGATAGAGAAACAAATCACTAATTTCATATGGAAGGGAAAGAACCCCCGGATAAGCAAAGCATTACTGAAAAAGAAGAAGAAAGTGGGAGGCCTCA +A00126:224:H7HMWDSX3:3:1113:8892:9878 ! 0 Test_seq 397096 - - NR A00126:224:H7HMWDSX3:3:1113:8892:9878161Test_seq3967016025S126M=396946396TTTTTTCTAGTATACCATTTTGATCAGTCTCTCCTGACATCCATTTTGATGTTTCTTTCCTGTCTTTTTAATGACCTTTTGCTTTGTTCATGTATGAGGTCCCTGATTTCATCCCACAGCTCATCTGGTCTTTGGTCATTAGTGTTCAGTGFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF,::FFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:126MC:Z:151MAS:i:126XS:i:0Yt:Z:NR A00126:224:H7HMWDSX3:3:1113:8892:987881Test_seq39694660151M=396701-396CATCTGATGGTCTATTCTGCAGTCGGCTCCTGGCCTTGTTCTGACTGATGATATTGAGCTTCTTCATTGTCTCTTTCCACAGATGTAGTTGATTCGATTCCTGTGTATTCTATCTGGTGAGGTGTGTATAGTCACAGTTTATTTTGTTGAAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:95A55MC:Z:25S126MAS:i:146XS:i:58Yt:Z:NR 0 60 CATCTGATGGTCTATTCTGCAGTCGGCTCCTGGCCTTGTTCTGACTGATGATATTGAGCTTCTTCATTGTCTCTTTCCACAGATGTAGTTGATTCGATTCCTGTGTATTCTATCTGGTGAGGTGTGTATAGTCACAGTTTATTTTGTTGAA +A00126:224:H7HMWDSX3:3:1113:7084:10441 ! 0 Test_seq 167640 - - NR A00126:224:H7HMWDSX3:3:1113:7084:10441161Test_seq167316721S130M=167490325ATCTGACACATTTAAGTGATCGATCAGGCGCTGGTCTGAGATGTCATAGCTGAATATTCTTTCCCAGTCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCGGGTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF,FFFFFFFFFFFFFFFFF:FFFFFFFF::FFFFFFFFFFFFFFFFFFFFF:FFFNM:i:3MD:Z:7A44A73T3MC:Z:151MAS:i:116XS:i:111XA:Z:Test_seq,-213589,130M21S,4;Test_seq,+400737,21S123M7S,5;Yt:Z:NR A00126:224:H7HMWDSX3:3:1113:7084:1044181Test_seq16749060151M=167316-325CTGTATTAGGGCTCCTAGGGTTGATCCTATTTTTTCTTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTACATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFNM:i:1MD:Z:102G48MC:Z:21S130MAS:i:146XS:i:96Yt:Z:NR 0 60 CTGTATTAGGGCTCCTAGGGTTGATCCTATTTTTTCTTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTACATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATC +A00126:224:H7HMWDSX3:3:1113:3025:10614 Test_seq 385792 Test_seq 386184 + - UU A00126:224:H7HMWDSX3:3:1113:3025:10614161Test_seq3857926015S136M=386034393GCCAGAAGGTTCCTTAATCCCTATCCCAAGGGATTTATCTCTAAAAAGAAATGCTGTTTTGTGCGTGGAATTCAGTCTGACTCATAGCACCCTGTATGACGAAGTAGAACTGCCCCACACGGTTTCCTAGGCTGTGATATTTACAAAAAAAFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FF:FF:FFFF,FFFFFFFFFFFFFFF,FFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:,FFFFFFFFFF:FFFFFFF,FFFNM:i:0MD:Z:136MC:Z:151MAS:i:136XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1113:3025:1061481Test_seq38603460151M=385792-393CGTTTCTTCTGTTTTTGCTCCTCATCTTCTTGCCTATTTGAGACATCACAATTTGTTTGTGCAATCCTGGAAATTCTCCTTGTTTCCACCCTCAACCCATCTCAGCCTGCTGCCTTCTTGTAGACCAGGGTCCCTGACTCTGGCCCATGCA:FFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF,F:FFF:FFFFFFFFFFFFFFFFFFFFFFF:::FFF:FFFFF:FFF:FFFFFF,FFFF,FFF:FFFF::F:FF:F:FFFFFFFFFNM:i:0MD:Z:151MC:Z:15S136MAS:i:151XS:i:0Yt:Z:UU 60 60 GCCAGAAGGTTCCTTAATCCCTATCCCAAGGGATTTATCTCTAAAAAGAAATGCTGTTTTGTGCGTGGAATTCAGTCTGACTCATAGCACCCTGTATGACGAAGTAGAACTGCCCCACACGGTTTCCTAGGCTGTGATATTTACAAAAAAA CGTTTCTTCTGTTTTTGCTCCTCATCTTCTTGCCTATTTGAGACATCACAATTTGTTTGTGCAATCCTGGAAATTCTCCTTGTTTCCACCCTCAACCCATCTCAGCCTGCTGCCTTCTTGTAGACCAGGGTCCCTGACTCTGGCCCATGCA +A00126:224:H7HMWDSX3:3:1113:6903:10942 ! 0 Test_seq 166317 - + NR A00126:224:H7HMWDSX3:3:1113:6903:1094281Test_seq16643460120M31S=166317-237AAGCTTTCATTTTATCCCGGAAACCTCTTTGCTTTTAGTCCAGTCCAATTAGGCTGACCTTCCTTGTATTGTGTGTTGTCTTTCCCTTCACCCAAAGCAGTTCTTATCTACTGATTGATCTAAAATTCAAGTCATATCTTGAAATTCACTAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFNM:i:0MD:Z:120MC:Z:151MAS:i:120XS:i:30Yt:Z:NR A00126:224:H7HMWDSX3:3:1113:6903:10942161Test_seq16631760151M=166434237GCCACAATTCAAAGTGGGATGCAGAATGTTTTCATAATAGAATTATTTTCCCCGTTGACTTAGAAGTCTCCTCAAACCATGTTCCCCAGACCCCAGCCCCTGCTCCGCTGACCTTTGAAGCTTTCATTTTATCCCGGAAACCTCTTTGCTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFNM:i:1MD:Z:49G101MC:Z:120M31SAS:i:146XS:i:32Yt:Z:NR 0 60 GCCACAATTCAAAGTGGGATGCAGAATGTTTTCATAATAGAATTATTTTCCCCGTTGACTTAGAAGTCTCCTCAAACCATGTTCCCCAGACCCCAGCCCCTGCTCCGCTGACCTTTGAAGCTTTCATTTTATCCCGGAAACCTCTTTGCTT +A00126:224:H7HMWDSX3:3:1113:32081:11365 Test_seq 168282 Test_seq 168607 + - UU A00126:224:H7HMWDSX3:3:1113:32081:1136597Test_seq16828260151M=168457326GCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGGTTTTCCCTGTATACAATCATGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCTTGCCAATCTGGATACCCTTTATTTCTTTGTCTAGCCTAATTGCCCTGFFFFFFFFF:FFF::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:,FFFFF,F,FFF:F:F:F:FFFFFFFFFFFFFFFFFFFFFFFNM:i:5MD:Z:0T54A7G0G18A67MC:Z:151MAS:i:130XS:i:92Yt:Z:UU A00126:224:H7HMWDSX3:3:1113:32081:11365145Test_seq16845760151M=168282-326AATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFFF::FFFFFFFFFFFNM:i:1MD:Z:38T112MC:Z:151MAS:i:146XS:i:91Yt:Z:UU 60 60 GCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGGTTTTCCCTGTATACAATCATGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCTTGCCAATCTGGATACCCTTTATTTCTTTGTCTAGCCTAATTGCCCTG AATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGG +A00126:224:H7HMWDSX3:3:1113:11822:11381 ! 0 Test_seq 168421 - + NR A00126:224:H7HMWDSX3:3:1113:11822:1138181Test_seq16865160127M24S=168421-357CTTTTCTGCATCAATTGATAAGATCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCGTACCTGGTATAAATCCCACTTGATCACTCTGTTCGGGCCTCTTCTTGCCTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFNM:i:3MD:Z:12T52T34A26MC:Z:151MAS:i:112XS:i:51Yt:Z:NR A00126:224:H7HMWDSX3:3:1113:11822:11381161Test_seq16842160151M=168651357CTAATTGCCCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCTGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:127M24SAS:i:151XS:i:69Yt:Z:NR 0 60 CTAATTGCCCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCTGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTA +A00126:224:H7HMWDSX3:3:1113:30861:11913 Test_seq 168282 Test_seq 168607 + - UU A00126:224:H7HMWDSX3:3:1113:30861:1191397Test_seq16828260151M=168457326GCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGGTTTTCCCTGTATACAATCATGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCTTGCCAATCTGGATACCCTTTATTTCTTTGTCTAGCCTAATTGCCCTGFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF,FF:FFFFFFNM:i:5MD:Z:0T54A7G0G18A67MC:Z:151MAS:i:130XS:i:92Yt:Z:UU A00126:224:H7HMWDSX3:3:1113:30861:11913145Test_seq16845760151M=168282-326AATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:38T112MC:Z:151MAS:i:146XS:i:91Yt:Z:UU 60 60 GCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGGTTTTCCCTGTATACAATCATGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCTTGCCAATCTGGATACCCTTTATTTCTTTGTCTAGCCTAATTGCCCTG AATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGG +A00126:224:H7HMWDSX3:3:1113:25021:12258 ! 0 Test_seq 384534 - - MU A00126:224:H7HMWDSX3:3:1113:25021:12258161Test_seq204882051M=384484179653GATAAACAGAGAAAAGATTGAAGTTGTCAAGGATTTCATTTGACTTGGATCFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFNM:i:2MD:Z:16T24T9MC:Z:51MAS:i:41XS:i:41XA:Z:Test_seq,+384484,51M,2;Test_seq,+449828,47M4S,2;Test_seq,-333028,51M,4;Yt:Z:MU A00126:224:H7HMWDSX3:3:1113:25021:1225881Test_seq3844841051M=204882-179653GATAAACGGAGAAAAAATTGAAGTTGTCAAGGATTTCATTTTACTTGGATCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:15G35MC:Z:51MAS:i:46XS:i:42XA:Z:Test_seq,-449828,47M4S,1;Test_seq,+136952,51M,3;Yt:Z:MU 0 10 GATAAACAGAGAAAAGATTGAAGTTGTCAAGGATTTCATTTGACTTGGATC GATAAACGGAGAAAAAATTGAAGTTGTCAAGGATTTCATTTTACTTGGATC +A00126:224:H7HMWDSX3:3:1113:30337:12696 Test_seq 169850 Test_seq 170349 + - UU A00126:224:H7HMWDSX3:3:1113:30337:1269697Test_seq16985060151M=170199500TTCTAGAGAATGTTCCATGTGCACTAGAAAAAAAAGTATACTTTGCAGCAGTTGGGTGGAGAGTTCTGTATAAGTCAATGAGGTCAAGTTGGTTGATTGTTGTAAGTAGGTCTTCCGTGTCTCTATTGAGCTTCTTACTGGATGTCCTGTCFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:4MD:Z:16C23T35T47G26MC:Z:151MAS:i:131XS:i:61Yt:Z:UU A00126:224:H7HMWDSX3:3:1113:30337:12696145Test_seq17019960151M=169850-500TTTAAGTCTAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCCTCTTCTTTTTTGCTTATTGTTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:63A87MC:Z:151MAS:i:146XS:i:75Yt:Z:UU 60 60 TTCTAGAGAATGTTCCATGTGCACTAGAAAAAAAAGTATACTTTGCAGCAGTTGGGTGGAGAGTTCTGTATAAGTCAATGAGGTCAAGTTGGTTGATTGTTGTAAGTAGGTCTTCCGTGTCTCTATTGAGCTTCTTACTGGATGTCCTGTC TTTAAGTCTAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCCTCTTCTTTTTTGCTTATTGTTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATA +A00126:224:H7HMWDSX3:3:1113:14434:14841 Test_seq 170093 Test_seq 170356 + - UU A00126:224:H7HMWDSX3:3:1113:14434:14841161Test_seq17009347151M=170206264TTGCAGCCCTGTCATTGGGTGCATAAATATTTAATATGGTTATGTCTTCCTGATCAATTGTCCCTTTTATCATTATATAGTGTCCTTCTTTATCCTTTGTGGTGGATTTAAGTCTAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:F:FFFFFFF:FFF,FFF::,NM:i:5MD:Z:14C7G20A58C0A47MC:Z:151MAS:i:126XS:i:101XA:Z:Test_seq,-38489,151M,10;Yt:Z:UU A00126:224:H7HMWDSX3:3:1113:14434:1484181Test_seq17020660151M=170093-264CTAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCCTCTTCTTTTTTGCTTATTGTTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGAT,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,F:FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:NM:i:1MD:Z:56A94MC:Z:151MAS:i:146XS:i:88Yt:Z:UU 47 60 TTGCAGCCCTGTCATTGGGTGCATAAATATTTAATATGGTTATGTCTTCCTGATCAATTGTCCCTTTTATCATTATATAGTGTCCTTCTTTATCCTTTGTGGTGGATTTAAGTCTAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCC CTAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCCTCTTCTTTTTTGCTTATTGTTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGAT +A00126:224:H7HMWDSX3:3:1113:14290:15405 ! 0 Test_seq 168462 - + NR A00126:224:H7HMWDSX3:3:1113:14290:15405145Test_seq16868860123M28S=168462-349TCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTTAAACAGATGGGAAAACCAAGGCCCAGAFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFF,FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:28T94MC:Z:151MAS:i:118XS:i:55Yt:Z:NR A00126:224:H7HMWDSX3:3:1113:14290:1540597Test_seq16846260151M=168688349GAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFF,FFFFFFFFFFFFFFFF:FF::,:FFFFF:FFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFF:FFFNM:i:1MD:Z:33T117MC:Z:123M28SAS:i:146XS:i:91Yt:Z:NR 0 60 GAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGA +A00126:224:H7HMWDSX3:3:1113:31087:16000 Test_seq 167317 Test_seq 167676 + - UU A00126:224:H7HMWDSX3:3:1113:31087:16000161Test_seq167317718S133M=167526360TGCTCTCTTTCTCCCTCAATCAGGCGCTGGTCTGAGATGTCATAGCTGAATATTCTTTCCCAGTCTGTAGGTGGTCTTTTTAGTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCTFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFF,F:FFFFFFFFF:F,FFFFFFF:FFF:F:FFFFFFFF::FFFFFFF::,FFFFFF:FFFF:FFFF:FFFF:F,FFFF,FFFF:F:FFFFFFFFFFFFFF:,F,F,FF:FFFFNM:i:3MD:Z:6A44A12C68MC:Z:151MAS:i:118XS:i:113XA:Z:Test_seq,-213585,133M18S,4;Yt:Z:UU A00126:224:H7HMWDSX3:3:1113:31087:1600081Test_seq16752652151M=167317-360TTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGGTATGCCAGCACCATTTGTTAAAAAGACTATCAFFFFF:FFFFFFF:FFFFFF:FFFFFF:FFFF::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF,FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFNM:i:1MD:Z:118T32MC:Z:18S133MAS:i:146XS:i:121XA:Z:Test_seq,+213362,98M2I23M2I26M,6;Yt:Z:UU 7 52 TGCTCTCTTTCTCCCTCAATCAGGCGCTGGTCTGAGATGTCATAGCTGAATATTCTTTCCCAGTCTGTAGGTGGTCTTTTTAGTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCT TTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGGTATGCCAGCACCATTTGTTAAAAAGACTATCA +A00126:224:H7HMWDSX3:3:1113:14027:16297 Test_seq 168274 Test_seq 168675 + - UU A00126:224:H7HMWDSX3:3:1113:14027:1629797Test_seq16827427151M=168525402GAGACTCTGCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGGTTTTCCGTGTACACGATCATGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCTTGCCAATCCGGATACCCTTTATTTCTTTGTCTAGCCTAAFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:FFFFFF,FFFFFNM:i:6MD:Z:8T54A4T3G18A28T30MC:Z:151MAS:i:121XS:i:106XA:Z:Test_seq,-212614,151M,9;Yt:Z:UU A00126:224:H7HMWDSX3:3:1113:14027:16297145Test_seq16852560151M=168274-402TCCATTTAGAGTGATATTGGCTGTTCGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATCFFF:FF,FFFFFFFFFF,FFFFFFFFF:F,FFFFFFF:F:FFFFFFF::F:FF:FFFFFFFF:FFF:FFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:25G125MC:Z:151MAS:i:146XS:i:0Yt:Z:UU 27 60 GAGACTCTGCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGGTTTTCCGTGTACACGATCATGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCTTGCCAATCCGGATACCCTTTATTTCTTTGTCTAGCCTAA TCCATTTAGAGTGATATTGGCTGTTCGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATC +A00126:224:H7HMWDSX3:3:1113:10493:17127 Test_seq 166938 Test_seq 167319 + - UU A00126:224:H7HMWDSX3:3:1113:10493:17127161Test_seq1669387151M=167169382GAGTGGGATTTCTGGGTTGTATGGTAGTTCTATTTCTAACTGTTTAAGATAACGCCAGATAGATTTCCAAAGTGGTTGTACCATTTTACATTCCCACCAGCAGTGTATGAGAGTTCCAATCTCTCCGCAGCCTCTCCAACATTTATTATTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFF:FFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFF:FF:FFFFFFFFFF:FFFFFF:FFF:FFFFF:FFF,FFFFNM:i:4MD:Z:60G29A27G7A24MC:Z:151MAS:i:131XS:i:126XA:Z:Test_seq,-213946,151M,5;Yt:Z:UU A00126:224:H7HMWDSX3:3:1113:10493:1712781Test_seq16716952151M=166938-382TAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAATCATGTAGATTTTAGAGATCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:74T76MC:Z:151MAS:i:146XS:i:121XA:Z:Test_seq,+213715,151M,6;Yt:Z:UU 7 52 GAGTGGGATTTCTGGGTTGTATGGTAGTTCTATTTCTAACTGTTTAAGATAACGCCAGATAGATTTCCAAAGTGGTTGTACCATTTTACATTCCCACCAGCAGTGTATGAGAGTTCCAATCTCTCCGCAGCCTCTCCAACATTTATTATTT TAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAATCATGTAGATTTTAGAGATC +A00126:224:H7HMWDSX3:3:1113:29857:18380 ! 0 Test_seq 248575 - - NR A00126:224:H7HMWDSX3:3:1113:29857:1838097Test_seq2482386037S114M=248425338GTCCACTGGCTCATGGAGCCCCTATGGACCTGGGATCTAAATGCTTTGCTACATGTACATGGAAACCCTGGTGGCGCAGTGGTTAAGAGCTACGTCTGCTAACCAAAAGGTCAGCAGTTCGAATCCCCCAGGTGCTTCCTTGGAAACTCTAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFNM:i:0MD:Z:114MC:Z:151MAS:i:114XS:i:39Yt:Z:NR A00126:224:H7HMWDSX3:3:1113:29857:18380145Test_seq24842560151M=248238-338TTTATATGTACAAAGCACAAGCTTTGGATTGAGATGGTTCCGAGTTCAAATTTCTTTACTAAATTTATGGTCTTAGGAAAGTTATTTAACATTTCTAAGGTTCAGTTTTCTCATTTATAAAATGATAATGATAATAGCTGTCTGTAGGCTTFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:37S114MAS:i:151XS:i:0Yt:Z:NR 0 60 TTTATATGTACAAAGCACAAGCTTTGGATTGAGATGGTTCCGAGTTCAAATTTCTTTACTAAATTTATGGTCTTAGGAAAGTTATTTAACATTTCTAAGGTTCAGTTTTCTCATTTATAAAATGATAATGATAATAGCTGTCTGTAGGCTT +A00126:224:H7HMWDSX3:3:1113:4444:18584 ! 0 Test_seq 167679 - - NR A00126:224:H7HMWDSX3:3:1113:4444:18584113Test_seq2136110126M25S=167529-46058AAAATCAAACACCTATGCTCATCTAAAGACTTCACCAAAAGAGTAAAAAGACCACCTACAGACTGGGAAAGAATTTTCAGCTATGACATCTCAGACCAGCGCCTGATCTCTAAAATCTACATGATTTAGATGAATGGTTGCTAGCATTTAAF:FF:FFFFF,FF,::FFFFFFFFFFF:FFFFFFFFFF:FFF,:F:F:FFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:3MD:Z:58T33C26T6MC:Z:151MAS:i:111XS:i:111XA:Z:Test_seq,+167298,25S126M,3;Test_seq,+400719,25S126M,5;Yt:Z:NR A00126:224:H7HMWDSX3:3:1113:4444:18584177Test_seq16752953151M=21361146058CATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATCTTTTFFFFFFFFFF,FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:147A3MC:Z:126M25SAS:i:147XS:i:122XA:Z:Test_seq,+213359,101M2I23M2I23M,6;Yt:Z:NR 0 53 CATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATCTTTT +A00126:224:H7HMWDSX3:3:1113:25536:18818 Test_seq 170292 Test_seq 170762 + - UU A00126:224:H7HMWDSX3:3:1113:25536:1881897Test_seq17029260151M=170625471GAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGTTCGTGTTTCTTTATCCAGTCCGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAATAAFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:63A87MC:Z:13S138MAS:i:146XS:i:76Yt:Z:UU A00126:224:H7HMWDSX3:3:1113:25536:18818145Test_seq1706256013S138M=170292-471GTTGTTATACCTCTTTGTGGTTACCTGAATATTTACCCCTATTTTTCTAAGTAAAAACCTAACTCGTATTGCTCTATATCGCCTTGTATCACTCTCCATATGGCAGTTCTATGCCACCTGTATTTAGTCCCTCTTTTTGATTATTGTGATCFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:10MD:Z:13T1T35T4C1T1G6A18C9A5T35MC:Z:151MAS:i:88XS:i:30Yt:Z:UU 60 60 GAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGTTCGTGTTTCTTTATCCAGTCCGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAATAA GTTGTTATACCTCTTTGTGGTTACCTGAATATTTACCCCTATTTTTCTAAGTAAAAACCTAACTCGTATTGCTCTATATCGCCTTGTATCACTCTCCATATGGCAGTTCTATGCCACCTGTATTTAGTCCCTCTTTTTGATTATTGTGATC +A00126:224:H7HMWDSX3:3:1113:26829:18897 Test_seq 168270 Test_seq 168611 + - UU A00126:224:H7HMWDSX3:3:1113:26829:18897161Test_seq16827060151M=168461342ACCTGAGACTCTTCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGGTTTTCCATGTATACGATCATGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCTTGCCAATCTGGGTACCCTTTATTTCTTTGTCTAGCFFFFFFFFFFFFFFFFFFFFFFFF:F,:FF:FFFFFFFFF,FFFFFFFFF,:FFFFFFFFFFFFFFF,:FFFFFFFF,FF:FFFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFF,FFFFFFFFFFFFF:F,FFFFFFFFFFFFFFNM:i:4MD:Z:1T74G18A31A23MC:Z:151MAS:i:134XS:i:91Yt:Z:UU A00126:224:H7HMWDSX3:3:1113:26829:1889781Test_seq16846160151M=168270-342AGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:34T116MC:Z:151MAS:i:146XS:i:91Yt:Z:UU 60 60 ACCTGAGACTCTTCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGGTTTTCCATGTATACGATCATGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCTTGCCAATCTGGGTACCCTTTATTTCTTTGTCTAGC AGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAG +A00126:224:H7HMWDSX3:3:1113:14055:19225 Test_seq 171575 Test_seq 171891 + - UU A00126:224:H7HMWDSX3:3:1113:14055:1922597Test_seq1715756014S137M=171741317TTGCCAATTGACTTAGTCTGCTCCTCTGACTTTCTATTGCGTTGTCAAATTCTGTAATTTTATTGTTAATCTTTTGGATTTCTACATGCTGTCTCTCTATGGATTCTTGCAACTTATTAATTTTTCCACTATGTTCTTGAATAATCTTTTTFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFF:F:FFNM:i:4MD:Z:8T23T83C13C6MC:Z:151MAS:i:117XS:i:78Yt:Z:UU A00126:224:H7HMWDSX3:3:1113:14055:19225145Test_seq17174160151M=171575-317CCTTGGCTTTTTCTGCAGTTATCCTAATTTCATTTGTGATATCTTTAAGCATTCTGTAAATTAGTTTTTTATATTCTGTATCTGATAATTCCAGGATTGTATCTTCATTTGGGAAAGATTTTGATTCTTTTGTTTGGGGGGTTGGAGAAGC:FFFFFFFF::FFFFFFF:FF,FFFFFFFFF,F:FFFFFFF:FFFFFFFFFFFFFFFFF,FFFFFFFFFF:FFFFF,FFFFFFFFFFF,FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:14S137MAS:i:151XS:i:25Yt:Z:UU 60 60 TTGCCAATTGACTTAGTCTGCTCCTCTGACTTTCTATTGCGTTGTCAAATTCTGTAATTTTATTGTTAATCTTTTGGATTTCTACATGCTGTCTCTCTATGGATTCTTGCAACTTATTAATTTTTCCACTATGTTCTTGAATAATCTTTTT CCTTGGCTTTTTCTGCAGTTATCCTAATTTCATTTGTGATATCTTTAAGCATTCTGTAAATTAGTTTTTTATATTCTGTATCTGATAATTCCAGGATTGTATCTTCATTTGGGAAAGATTTTGATTCTTTTGTTTGGGGGGTTGGAGAAGC +A00126:224:H7HMWDSX3:3:1113:15085:20322 ! 0 Test_seq 169286 - + NR A00126:224:H7HMWDSX3:3:1113:15085:20322145Test_seq16961960128M23S=169286-461TTTCATTCTCGTTGCTTTCTATGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTATTGTTCATTTTCCAAGTATTTGATTTCTTTTCCCTCGTTTTTCTGTTATTGATCGATCGTTTCTTTCCATGFFF,FF:FFFFF::FFFFF:FF:FF::FF:FFFF:FFFFFFFFFF,FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFF:FFFF:FFNM:i:4MD:Z:9T11A22G70A12MC:Z:151MAS:i:108XS:i:0Yt:Z:NR A00126:224:H7HMWDSX3:3:1113:15085:2032297Test_seq16928660151M=169619461GGGTTATTTGTTTCCTTTCCTGTATTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTFFF,FF,,FFFFFF:FFFFF,F::F,FFF:F:FFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:F,:FFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFF:FF,F:FFF:FF:FFFF,F:FFFFFF:::NM:i:1MD:Z:23T127MC:Z:128M23SAS:i:146XS:i:104Yt:Z:NR 0 60 GGGTTATTTGTTTCCTTTCCTGTATTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTT +A00126:224:H7HMWDSX3:3:1113:5945:21277 Test_seq 168437 Test_seq 168809 + - UU A00126:224:H7HMWDSX3:3:1113:5945:2127797Test_seq16843760151M=168659373GGACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTFF:FFFFFFFFFFFF,FFF:FFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:F,FFFFFFFFFFFFFFFF,FFFFFFFFFF,FFFNM:i:1MD:Z:58T92MC:Z:151MAS:i:146XS:i:85Yt:Z:UU A00126:224:H7HMWDSX3:3:1113:5945:21277145Test_seq16865960151M=168437-373CATCAATTGATAAGATCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGAT:F:F:FFFFFFFFFFF,FFF::F:FFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:FFFFF:FF,FFFFFFFFFFFFF:FFFFFF:FFFFFFFFFF:FFFFFFFFFFFNM:i:2MD:Z:4T52T93MC:Z:151MAS:i:141XS:i:55Yt:Z:UU 60 60 GGACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTT CATCAATTGATAAGATCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGAT +A00126:224:H7HMWDSX3:3:1113:8675:22780 ! 0 Test_seq 168669 - - NR A00126:224:H7HMWDSX3:3:1113:8675:2278097Test_seq1683476024S127M=168519323CATGAGAGTAAAGAAAATGATCGATCATGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCTTACCAATCTGGATACCCTTTACTTCTCTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGTACGATGTTGAGTAAGAGCGGTGATAAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFNM:i:5MD:Z:18A21G19T4T45A15MC:Z:151MAS:i:102XS:i:46Yt:Z:NR A00126:224:H7HMWDSX3:3:1113:8675:22780145Test_seq16851960151M=168347-323GTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTACATCTATTGATFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:139G11MC:Z:24S127MAS:i:146XS:i:84Yt:Z:NR 0 60 GTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTACATCTATTGAT +A00126:224:H7HMWDSX3:3:1113:5159:23359 ! 0 Test_seq 239109 - - NR A00126:224:H7HMWDSX3:3:1113:5159:2335997Test_seq2387196021S130M=238959391TAGTCCAATCCCTATTTGATCGATCAAAAGATATTGACCTCCTAGGGTTTTTAGCATGTATTTTCAAATCATCCTAAAGGAAAGTAATACAAACTTATAGTCTCACCTGTTGTGTGAAAAGACCTGTTTTTCAAGATGCTTGTCTACACAGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFNM:i:0MD:Z:130MC:Z:151MAS:i:130XS:i:0Yt:Z:NR A00126:224:H7HMWDSX3:3:1113:5159:23359145Test_seq23895960151M=238719-391GGTCTTTGTCCATTGTTCTACTGATATTAGTCTCTTTTAGTTGATTCACAGGACTTGAGAGGTTATTGCTATTAATCTCTATCACATATTTTGCAAATATATTCCTCAGTTTCTTTACTTGCCTTTTATTTATTTTTTCATTTTAAGGTTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFNM:i:0MD:Z:151MC:Z:21S130MAS:i:151XS:i:0Yt:Z:NR 0 60 GGTCTTTGTCCATTGTTCTACTGATATTAGTCTCTTTTAGTTGATTCACAGGACTTGAGAGGTTATTGCTATTAATCTCTATCACATATTTTGCAAATATATTCCTCAGTTTCTTTACTTGCCTTTTATTTATTTTTTCATTTTAAGGTTT +A00126:224:H7HMWDSX3:3:1113:32551:24267 Test_seq 258024 Test_seq 258280 + - UU A00126:224:H7HMWDSX3:3:1113:32551:2426797Test_seq2580246016S135M=258130257CTCCTTCAAACTGATCGATCACCAACTTCATATGGAAAGGGAAGCATCTCTGGATAAGTAAATCATTACTGAAAAAGAAGAACAAAGTGAAAGGCCTCACACTGTCTGATTTTAGAACCTATTATAATGCCACAGTAGTCAAAACAGCTTGFFFFFFFF:FFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:135MC:Z:151MAS:i:135XS:i:32Yt:Z:UU A00126:224:H7HMWDSX3:3:1113:32551:24267145Test_seq25813060151M=258024-257TATAATGCCACAGTAGTCAAAACAGCTTGGTACTGTTACAGCAACAGATACATAGACCAATGGAACAGAATTGAGAATCCAGACATAAATTCGTCCACTTAAGAGCAGCTGATATTTGACAAAGGCCCAAAGTCCATTAATTGGGGAAAAGFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFNM:i:0MD:Z:151MC:Z:16S135MAS:i:151XS:i:51Yt:Z:UU 60 60 CTCCTTCAAACTGATCGATCACCAACTTCATATGGAAAGGGAAGCATCTCTGGATAAGTAAATCATTACTGAAAAAGAAGAACAAAGTGAAAGGCCTCACACTGTCTGATTTTAGAACCTATTATAATGCCACAGTAGTCAAAACAGCTTG TATAATGCCACAGTAGTCAAAACAGCTTGGTACTGTTACAGCAACAGATACATAGACCAATGGAACAGAATTGAGAATCCAGACATAAATTCGTCCACTTAAGAGCAGCTGATATTTGACAAAGGCCCAAAGTCCATTAATTGGGGAAAAG +A00126:224:H7HMWDSX3:3:1113:27046:26068 Test_seq 168498 Test_seq 168675 + - UU A00126:224:H7HMWDSX3:3:1113:27046:2606897Test_seq16849860151M=168543178TCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGGACTTTGTCAAATFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:138A12MC:Z:133M18SAS:i:146XS:i:91Yt:Z:UU A00126:224:H7HMWDSX3:3:1113:27046:26068145Test_seq16854360133M18S=168498-178GGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGGACTTTGTCAAATGCCTTTTCTGCATCAATTGATAAGATCGATCCCAGTGGTCATGGCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:2MD:Z:93A26T12MC:Z:151MAS:i:123XS:i:75Yt:Z:UU 60 60 TCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGGACTTTGTCAAAT GGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGGACTTTGTCAAATGCCTTTTCTGCATCAATTGATAAGATCGATCCCAGTGGTCATGGC +A00126:224:H7HMWDSX3:3:1113:24804:26537 Test_seq 170240 Test_seq 170549 + - UU A00126:224:H7HMWDSX3:3:1113:24804:2653797Test_seq17024060151M=170399310CTCCTCTTCTTTTTTGCTTATTGTTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCGTGACTCTCTGTCTFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF:FFFFF,FFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFF:FFF,F:FFFFFFF,FFFFFFFF,FFFFFFF,F::F:F::FF:::FFFFFF,FFFNM:i:1MD:Z:22A128MC:Z:151MAS:i:146XS:i:78Yt:Z:UU A00126:224:H7HMWDSX3:3:1113:24804:26537145Test_seq17039960151M=170240-310GTGCATTTAGTCCATTTACATTCAGTGTAATTATAGCTAAATAAGTTTTTAGTGTTGTCATTTTGATGCCTTTTCATGTGTGTTGTTGGCAATTTCATTTTTCCACATACTTTTTTGTGCTGAGGCGTTTTTCTTAGTAAATTGTGAGATCFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:7MD:Z:25C10A17C35C0T32A14G11MC:Z:151MAS:i:116XS:i:29Yt:Z:UU 60 60 CTCCTCTTCTTTTTTGCTTATTGTTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCGTGACTCTCTGTCT GTGCATTTAGTCCATTTACATTCAGTGTAATTATAGCTAAATAAGTTTTTAGTGTTGTCATTTTGATGCCTTTTCATGTGTGTTGTTGGCAATTTCATTTTTCCACATACTTTTTTGTGCTGAGGCGTTTTTCTTAGTAAATTGTGAGATC +A00126:224:H7HMWDSX3:3:1113:21576:28995 Test_seq 221041 Test_seq 221448 + - UU A00126:224:H7HMWDSX3:3:1113:21576:28995161Test_seq22104160151M=221298408TCAGCCCAGTAATTTGGTCCCTCAGGGAGTTTAGATGTGTCTGTGGACCTTCCATGACCTTGCCTTGTATAAGCTTTTCTGGCTTCCCCAGTATTGTGGACTGTCTTACCCTTCACCTAAGTTACCATTTATCTATTGTCTAGTGTTTTTCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:43Yt:Z:UU A00126:224:H7HMWDSX3:3:1113:21576:2899581Test_seq22129860151M=221041-408CTGTGATTGATTTATTTCACTCAGCATAATGCCCTCCAGATTCATCTATGTTGTGAGCTGCTTTGCAGATTCGTCATTGTTCTTTATCGTCACATAGTACTCCATTGTGTGTATGTACCACAATTTGTTTATCCATTCATCTGTTGATAGGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:42Yt:Z:UU 60 60 TCAGCCCAGTAATTTGGTCCCTCAGGGAGTTTAGATGTGTCTGTGGACCTTCCATGACCTTGCCTTGTATAAGCTTTTCTGGCTTCCCCAGTATTGTGGACTGTCTTACCCTTCACCTAAGTTACCATTTATCTATTGTCTAGTGTTTTTC CTGTGATTGATTTATTTCACTCAGCATAATGCCCTCCAGATTCATCTATGTTGTGAGCTGCTTTGCAGATTCGTCATTGTTCTTTATCGTCACATAGTACTCCATTGTGTGTATGTACCACAATTTGTTTATCCATTCATCTGTTGATAGG +A00126:224:H7HMWDSX3:3:1113:32172:31751 Test_seq 167149 Test_seq 167515 + - UU A00126:224:H7HMWDSX3:3:1113:32172:3175197Test_seq16714942151M=167384367ATTTGCATTTCTCTAATGGCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FF:F:FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF,F:FFFFF:FFF:FFFFFFFF,FF,F:,:FFF,FF:FFF:FF:::,FFFFFFFFF,:FFFFFFFF:NM:i:1MD:Z:94T56MC:Z:132M19SAS:i:146XS:i:126XA:Z:Test_seq,-213735,151M,5;Yt:Z:UU A00126:224:H7HMWDSX3:3:1113:32172:31751145Test_seq16738431132M19S=167149-367TTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTGATCTGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATGCCCTGTATTAGGGCTCCTAGGGTTGATCACTCAGATAAGAGAAGGTCF:FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFF,FFFFFF:FF:FF:,FFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF::FFFF:FFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFFFNM:i:1MD:Z:54T77MC:Z:151MAS:i:127XS:i:113XA:Z:Test_seq,+213528,28S123M,2;Yt:Z:UU 42 31 ATTTGCATTTCTCTAATGGCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAA TTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTGATCTGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATGCCCTGTATTAGGGCTCCTAGGGTTGATCACTCAGATAAGAGAAGGTC +A00126:224:H7HMWDSX3:3:1113:25174:32252 Test_seq 167567 Test_seq 167973 + - UU A00126:224:H7HMWDSX3:3:1113:25174:3225297Test_seq1675673214S137M=167823407TATGGGATGTTGAGTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATTATTTCCCCGATTGACTGACACTGGTCCTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFNM:i:2MD:Z:108C8A19MC:Z:151MAS:i:127XS:i:112XA:Z:Test_seq,-213335,125M2I10M14S,5;Yt:Z:UU A00126:224:H7HMWDSX3:3:1113:25174:32252145Test_seq16782360151M=167567-407ATAATAGGTTCTGAAATCAGGTAGAGTGAGGCCTCCCACTTTCTTCTTCTTTTTCAGTAATGCTTTGCTTATCCGGGGGTTCTTTCCCTTCCATATGAAATTAGTGATTTGTTTCTCTATCCCCTTAAAATATGACATTGGTATTTGGATCFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:150T0MC:Z:14S137MAS:i:150XS:i:96Yt:Z:UU 32 60 TATGGGATGTTGAGTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATTATTTCCCCGATTGACTGACACTGGTCCT ATAATAGGTTCTGAAATCAGGTAGAGTGAGGCCTCCCACTTTCTTCTTCTTTTTCAGTAATGCTTTGCTTATCCGGGGGTTCTTTCCCTTCCATATGAAATTAGTGATTTGTTTCTCTATCCCCTTAAAATATGACATTGGTATTTGGATC +A00126:224:H7HMWDSX3:3:1113:29921:33176 ! 0 Test_seq 74365 - - NR A00126:224:H7HMWDSX3:3:1113:29921:33176161Test_seq740976027S124M=74215269CCTGATGGCACAGTGGCTTAGCTCTCAACTCTCTCCTAGGCACTGATGATTGAGTAATGAACACTCTAGACATGTTCCCTGTCTTTACTTACAATCTATTGGGGAAATGGACATCTAATCAAGCAGTTATAACACAACATGCTATGAAGGAFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:F:FFFF,F:FFFFF:F:FFF:FFFFFFFFFFFFFFFF,FFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFF:FFFF:FFFFFFFFFFFFFNM:i:0MD:Z:124MC:Z:151MAS:i:124XS:i:0Yt:Z:NR A00126:224:H7HMWDSX3:3:1113:29921:3317681Test_seq7421560151M=74097-269GAAGGATCATAGGAAAGGTATAGGTTACTGCTGGAAGGAGCATCTTAACAAGAGTTGGGAGTCCAGGGAAGCCTTCTGGAGACCAAATCTGTTACCATCCGACTCATAGTGACCCTATAGGACAGAATAGAACTCCCCCATAGGGTTTCCAFFFFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFF,FFFFFFFFFFF,FF:FFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:27S124MAS:i:151XS:i:0Yt:Z:NR 0 60 GAAGGATCATAGGAAAGGTATAGGTTACTGCTGGAAGGAGCATCTTAACAAGAGTTGGGAGTCCAGGGAAGCCTTCTGGAGACCAAATCTGTTACCATCCGACTCATAGTGACCCTATAGGACAGAATAGAACTCCCCCATAGGGTTTCCA +A00126:224:H7HMWDSX3:3:1113:23393:35117 Test_seq 170266 Test_seq 170325 + - UU A00126:224:H7HMWDSX3:3:1113:23393:3511797Test_seq1702663960M=17026660GCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:60MC:Z:60MAS:i:60XS:i:48Yt:Z:UU A00126:224:H7HMWDSX3:3:1113:23393:35117145Test_seq1702663960M=170266-60GCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:60MC:Z:60MAS:i:60XS:i:48Yt:Z:UU 39 39 GCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGG GCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGG +A00126:224:H7HMWDSX3:3:1113:23086:35149 Test_seq 170266 Test_seq 170325 + - UU A00126:224:H7HMWDSX3:3:1113:23086:3514997Test_seq1702663960M=17026660GCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:60MC:Z:60MAS:i:60XS:i:48Yt:Z:UU A00126:224:H7HMWDSX3:3:1113:23086:35149145Test_seq1702663960M=170266-60GCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:60MC:Z:60MAS:i:60XS:i:48Yt:Z:UU 39 39 GCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGG GCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGG +A00126:224:H7HMWDSX3:3:1113:25057:36714 Test_seq 168238 Test_seq 168557 + - UU A00126:224:H7HMWDSX3:3:1113:25057:36714161Test_seq1682382415S136M=168407320CCCCAAGGTGTATTTAATCCAAGTGATTTTTGTATGTTTATTTTATAACCTGACACTCTGCCAAACTCTTCTATTAGTGTCAGTAGTTTTCTGGAGGATTCCTTAGGGTTTTCCATGTATACGATCATGTCATCTGCAAATAGTGATAGCTFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:F::FFFFFF:FFFFFFFFFFF:FFFFFFFF:FFFFFFFFF:F,FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:6MD:Z:33T4G5T18T44G18A8MC:Z:151MAS:i:106XS:i:93XA:Z:Test_seq,-212673,8S128M15S,7;Yt:Z:UU A00126:224:H7HMWDSX3:3:1113:25057:3671481Test_seq16840760151M=168238-320TTTCTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGC,FFFF:FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF,:FFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:88T62MC:Z:15S136MAS:i:146XS:i:78Yt:Z:UU 24 60 CCCCAAGGTGTATTTAATCCAAGTGATTTTTGTATGTTTATTTTATAACCTGACACTCTGCCAAACTCTTCTATTAGTGTCAGTAGTTTTCTGGAGGATTCCTTAGGGTTTTCCATGTATACGATCATGTCATCTGCAAATAGTGATAGCT TTTCTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGC +A00126:224:H7HMWDSX3:3:1114:11785:1485 Test_seq 170257 Test_seq 170704 + - UU A00126:224:H7HMWDSX3:3:1114:11785:148597Test_seq17025760151M=170566448TTATTGTTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCGTGACTCTCTGTCTCTTTATTGGTGCATTTAFFFFFFFF:FFFFFFFFFFF:F:FFFF:FFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFF:FFFFFFF:F:FFFFFFFF:FFFFFFFFF,F:FFF,FFF:FFFFFFFF:FF:F:FFF:FFFFFFFFFFF:FFFFFFFFFF,FF:FFF,FNM:i:1MD:Z:5A145MC:Z:32M12I107MAS:i:146XS:i:80Yt:Z:UU A00126:224:H7HMWDSX3:3:1114:11785:1485145Test_seq1705666032M12I107M=170257-448TTGACTTTATGTTAGTTGAGTCGTTACGTTTTTCTTGGCTTTTATCTTGAGTTATGGAGTTGTTATACCTTTTTGTGGTTACCTTATTATTTACCCCTATTTTTCTAAGTAAAAACCTAACTTGTATCGTTCTATATCGCCTTGTATGACTFFFFF:FFFFFFFFFFFFFFFF:FFFFF:FFF:FFF::F,FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:20MD:Z:2A10T32A3A3T64G6A8C3MC:Z:151MAS:i:84XS:i:37Yt:Z:UU 60 60 TTATTGTTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCGTGACTCTCTGTCTCTTTATTGGTGCATTTA TTGACTTTATGTTAGTTGAGTCGTTACGTTTTTCTTGGCTTTTATCTTGAGTTATGGAGTTGTTATACCTTTTTGTGGTTACCTTATTATTTACCCCTATTTTTCTAAGTAAAAACCTAACTTGTATCGTTCTATATCGCCTTGTATGACT +A00126:224:H7HMWDSX3:3:1114:14986:3239 ! 0 Test_seq 333153 - + NR A00126:224:H7HMWDSX3:3:1114:14986:323981Test_seq33337760124M27S=333153-348CTGCAGTATTCCCTTGTTCTGTCTGACCAACTGCCTCTTGATCTATGTAAAGGTTCCTTATGAGCACAATTAAGTGTTCTGGAATTCCCATTCTTCGCAGTGTTATCCAGAGTTTGTTATGATCGATCCAGGCAGGTAAACCTTCAGGCCA,:FFFFFF,:FFF:FF:,FFFFF:F,:F:,FFF,:FF,F:,FFFFFF:F,FFF::FFFFFFFFFFFFF:FF,FFFFFFFFFF:F,:FFFFFFF:FFFF:FFFF:F:FFFFFFFFF::FF:F:FFFFFFF,F:FFFF,FFFFFFFFFF:FFFNM:i:1MD:Z:0A123MC:Z:151MAS:i:123XS:i:62Yt:Z:NR A00126:224:H7HMWDSX3:3:1114:14986:3239161Test_seq33315332151M=333377348GTGGTCTTTGATCTTCATTAGTAAGTGCTTCAAGTCCTCTTCACTTTCAGCAAGCAAGGTTGTGTCATCTGCATAACGCAGGTTGTTAAAGAGTCTTCCTCCAAACCTGATGCCCCGTTCTTCTTCATATAGTCCAGCTTCTCGTATTATTF:FF,FFF:FFFFFFFFFF:FFFFF:FFFFFFFFFFFFFF,FFFFFFFFF:FFF:F:FFFFFF:FFFF:FFFFFFF:F:FFFFFFFFFF:FFFFF:F,::::FFFF:FF:F,,F:FF,FFFFFF:,FFFFF:FFFFF,,:FF:FFFF,,F:NM:i:0MD:Z:151MC:Z:124M27SAS:i:151XS:i:136XA:Z:Test_seq,+315878,151M,3;Yt:Z:NR 0 32 GTGGTCTTTGATCTTCATTAGTAAGTGCTTCAAGTCCTCTTCACTTTCAGCAAGCAAGGTTGTGTCATCTGCATAACGCAGGTTGTTAAAGAGTCTTCCTCCAAACCTGATGCCCCGTTCTTCTTCATATAGTCCAGCTTCTCGTATTATT +A00126:224:H7HMWDSX3:3:1114:26268:3333 Test_seq 435684 Test_seq 436086 + - UU A00126:224:H7HMWDSX3:3:1114:26268:3333161Test_seq43568460151M=435936403CTGAGCAATAATCACCGCCCATGGGGCAGTTCTGCTCTGCCCTATAGGGTTGCTATGAGTCGGAATCAACTCCAGGGCAATAGGGTTTTTTTGGTTTTACCATCACCTTGAATCAGAAACAACTAGACAGCAATAGCAAATAGCTAAAAGTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:31Yt:Z:UU A00126:224:H7HMWDSX3:3:1114:26268:333381Test_seq43593660151M=435684-403TTATCGCAGTTGTAAATACCTTTTCTCTCTTTTTCAGGACAGACTGGCAAATCTTTGAATGTCTAATGTACACCTATGTATGATAATCTCTTTTCCTCCAGGAAAAAATTTCTGCCTTGAAAAAGCAAGCGAATCATATTGTCCAGCAGGCFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU 60 60 CTGAGCAATAATCACCGCCCATGGGGCAGTTCTGCTCTGCCCTATAGGGTTGCTATGAGTCGGAATCAACTCCAGGGCAATAGGGTTTTTTTGGTTTTACCATCACCTTGAATCAGAAACAACTAGACAGCAATAGCAAATAGCTAAAAGT TTATCGCAGTTGTAAATACCTTTTCTCTCTTTTTCAGGACAGACTGGCAAATCTTTGAATGTCTAATGTACACCTATGTATGATAATCTCTTTTCCTCCAGGAAAAAATTTCTGCCTTGAAAAAGCAAGCGAATCATATTGTCCAGCAGGC +A00126:224:H7HMWDSX3:3:1114:5791:5008 Test_seq 70329 Test_seq 71380 - - UR A00126:224:H7HMWDSX3:3:1114:5791:50082145Test_seq712266020H131M=71230155GATCCTAGAATGGGCTGCTTCCACCTGCATGCTGCCCTCTCGCCATGCTTTTGCGGCTGAGGATTTGGTGTCTGTGACGTGATCATTGGACGTGACCTTGACTGAATTGGGCCAAGTTGGCTCCACGTCTCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF,FFFFFFFFFFFFF:FFFNM:i:0MD:Z:131MC:Z:151MAS:i:131XS:i:0SA:Z:Test_seq,70310,-,131S20M,6,0;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1114:5791:5008113Test_seq703106131S20M=712301052GAGACGTGGAGCCAACTTGGCCCAATTCAGTCAAGGTCACGTCCAATGATCACGTCACAGACACCAAATCCTCAGCCGCAAAAGCATGGCGAGAGGGCAGCATGCAGGTGGAAGCAGCCCATTCTAGGATCAATCCAGGTTCTGCCCCTCCFFF:FFFFFFFFFFFFF,FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:20MC:Z:151MAS:i:20XS:i:0SA:Z:Test_seq,71226,+,20S131M,60,0;Yt:Z:UR A00126:224:H7HMWDSX3:3:1114:5791:5008177Test_seq7123060151M=70310-1052CTAGAATGGGCTGCTTCCACCTGCATGCTGCCCTCTCGCCATGCTTTTGCGGCTGAGGATTTGGTGTCTGTGACGTGATCATTGGACGTGACCTTGACTGAATTGGGCCAAGTTGGCTCCACGTCTCTCCCAGCCTCTAGGAGCTTATGAGFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:131S20MAS:i:151XS:i:0Yt:Z:UR 6 60 GAGACGTGGAGCCAACTTGGCCCAATTCAGTCAAGGTCACGTCCAATGATCACGTCACAGACACCAAATCCTCAGCCGCAAAAGCATGGCGAGAGGGCAGCATGCAGGTGGAAGCAGCCCATTCTAGGATCAATCCAGGTTCTGCCCCTCC CTAGAATGGGCTGCTTCCACCTGCATGCTGCCCTCTCGCCATGCTTTTGCGGCTGAGGATTTGGTGTCTGTGACGTGATCATTGGACGTGACCTTGACTGAATTGGGCCAAGTTGGCTCCACGTCTCTCCCAGCCTCTAGGAGCTTATGAG +A00126:224:H7HMWDSX3:3:1114:24153:6778 Test_seq 166090 Test_seq 166553 + - UU A00126:224:H7HMWDSX3:3:1114:24153:677897Test_seq16609060151M=166418464GTTGTCTGTTGTGCTCCCTGACAGGGCAGACATCGATTGTGGCCGGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCTTAGTTGTCGTGTGACCTTGGTGTTCTTCCTFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF,F:F:FFFFF,FF::FFFFFFFFFFFF:FFFFFFFFFF:FF,:NM:i:1MD:Z:134G16MC:Z:136M15SAS:i:146XS:i:85Yt:Z:UU A00126:224:H7HMWDSX3:3:1114:24153:6778145Test_seq16641860136M15S=166090-464GCTCCGCTGACCTTTGAAGCTTTCATTTTATCCCGGAAATCTCTTTGCTTTTAGTCCAGTCCAGTTGGGCTGACCTTCCTTGTATTGAGTGTTGTCTTTCCCTTCACCCAAAGCAGTTCTTATCTACTGATTGATCGATCCACCGTCAATCFFFFFFF,FFFFFFFFFFFFFFFF:F:FF:FFFFFFFF,FFF:FFFFFF,FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FF:FFFF,FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFNM:i:4MD:Z:39C23A2A20T48MC:Z:151MAS:i:116XS:i:40Yt:Z:UU 60 60 GTTGTCTGTTGTGCTCCCTGACAGGGCAGACATCGATTGTGGCCGGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCTTAGTTGTCGTGTGACCTTGGTGTTCTTCCT GCTCCGCTGACCTTTGAAGCTTTCATTTTATCCCGGAAATCTCTTTGCTTTTAGTCCAGTCCAGTTGGGCTGACCTTCCTTGTATTGAGTGTTGTCTTTCCCTTCACCCAAAGCAGTTCTTATCTACTGATTGATCGATCCACCGTCAATC +A00126:224:H7HMWDSX3:3:1114:29423:7827 Test_seq 15892 Test_seq 168477 - + UR A00126:224:H7HMWDSX3:3:1114:29423:78272193Test_seq16868560126M25H=168477-334TTGTCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:2MD:Z:2A28T94MC:Z:151MAS:i:118XS:i:58SA:Z:Test_seq,15873,-,131S20M,6,0;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1114:29423:7827145Test_seq158736131S20M=168477152586TTGTCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTAGTCACCCTTTGAAATCTTCTGTTCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFNM:i:0MD:Z:20MC:Z:151MAS:i:20XS:i:0SA:Z:Test_seq,168685,-,126M25S,60,2;Yt:Z:UR A00126:224:H7HMWDSX3:3:1114:29423:782797Test_seq16847760151M=15873-152586GCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:18T132MC:Z:131S20MAS:i:146XS:i:92Yt:Z:UR 6 60 TTGTCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTAGTCACCCTTTGAAATCTTCTGTTC GCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAAT +A00126:224:H7HMWDSX3:3:1114:22453:9032 Test_seq 414176 Test_seq 414453 + - UU A00126:224:H7HMWDSX3:3:1114:22453:903297Test_seq41417660151M=414303278TGCCTCTTTGTCCACCTAGAAGCTTAGACAAGAAACCTGGAAGTCAGTCTTGATCCTTCTTCGTCCATCACCCCCACATCCAGTCGGTTATCAAAATACTTCTCAATTACTTCAACCTCTTCGCATCTCCACTGACAGCATTCTCCCCTCAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF,FF:FFFFF::FF:FF,FFFFFFFFFFF:F:FFFF,FFFFFFFFFFFFFF,,FF,FFFF:FFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1114:22453:9032145Test_seq41430360151M=414176-278TCCACTGACAGCATTCTCCCCTCAATCACCATCGACTCGCATTAAGCCCCATTTAGTTCCATTTCCCTTTCAATCCATCTCTTCACAGCAGCCAGGGTGATTTAAAAAAATATATAAATTACAGCACATCACTCTGGTGTGTGGCCCACTGFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU 60 60 TGCCTCTTTGTCCACCTAGAAGCTTAGACAAGAAACCTGGAAGTCAGTCTTGATCCTTCTTCGTCCATCACCCCCACATCCAGTCGGTTATCAAAATACTTCTCAATTACTTCAACCTCTTCGCATCTCCACTGACAGCATTCTCCCCTCA TCCACTGACAGCATTCTCCCCTCAATCACCATCGACTCGCATTAAGCCCCATTTAGTTCCATTTCCCTTTCAATCCATCTCTTCACAGCAGCCAGGGTGATTTAAAAAAATATATAAATTACAGCACATCACTCTGGTGTGTGGCCCACTG +A00126:224:H7HMWDSX3:3:1114:27959:9392 Test_seq 171239 Test_seq 171657 + - UU A00126:224:H7HMWDSX3:3:1114:27959:939297Test_seq17123960151M=171507419GTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATGGGATTCGATGAGCATCTTGGATAGATATCCTTTCGTCTTTCATGATGTCAGGGAAGTTTTGTGTCAGGAGTTCTTCAACTATTTTCTCTGF,,FF:FFFFFFF,:FF:FF:FFFFFFF:FFF,FFFFFFFFFFFFFFFFFFFF,F,FFFFFF,FFFFFFFFFFFFF,FFFFFFFFF:FF:FFFFFFFF:FFF,FFFF:FFFFFFFFFFFFF:FFFFF,,:,:FFFFFFFFFF,FFFFFFFFNM:i:1MD:Z:63G87MC:Z:151MAS:i:146XS:i:65Yt:Z:UU A00126:224:H7HMWDSX3:3:1114:27959:9392145Test_seq17150760151M=171239-419TTTTTTCAGCTATGTTGCAGTTGATTCCCTGGTCCTCCAGAAGTCCCAGTCTACATTCTAATTGGTCGAGTCTGCTCCTCTGACTTTCTATTGCGTTGTCTAATTCTGTAATTTTATTGTTAATCTTTTGGATTTCTACATGCTGTCTCTCFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:3MD:Z:17G46C11T74MC:Z:151MAS:i:136XS:i:47Yt:Z:UU 60 60 GTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATGGGATTCGATGAGCATCTTGGATAGATATCCTTTCGTCTTTCATGATGTCAGGGAAGTTTTGTGTCAGGAGTTCTTCAACTATTTTCTCTG TTTTTTCAGCTATGTTGCAGTTGATTCCCTGGTCCTCCAGAAGTCCCAGTCTACATTCTAATTGGTCGAGTCTGCTCCTCTGACTTTCTATTGCGTTGTCTAATTCTGTAATTTTATTGTTAATCTTTTGGATTTCTACATGCTGTCTCTC +A00126:224:H7HMWDSX3:3:1114:13259:9518 Test_seq 305947 Test_seq 306239 + - UU A00126:224:H7HMWDSX3:3:1114:13259:9518161Test_seq30594760151M=306104293GAGTGTTTTTCTAAGTAGTCAAGTGACTGTTCACTTAGATTTAAGACATTTCTCTTCTCCTGTCATCAGCATTAGAGCCCAGATACTAAAGAGGCAAAAGTCATGGAAACCACTGGAGGGCCATTATCTTTCCTCATCTCCCCCATGTTGCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:F:FFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:136M15SAS:i:151XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1114:13259:951881Test_seq30610460136M15S=305947-293TCATCACAAAGGTCTGCGGTTGGTAAAGAGAGGAACTGGATGAAAGGGTTTTGATGGGTTGTTCAAATCTACCACCAAGGCTGTAAACAACTCCCTGAATGAAAGCATCATACTTACTCTTCTTCTAGCCTGGATTTAAAAAAGGGGGGAAFFF:FFFFF,FFFFFFFFFF:FFFFFFFFFFF:FFF,FFFF:FF:FFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFF,FF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:136MC:Z:151MAS:i:136XS:i:0Yt:Z:UU 60 60 GAGTGTTTTTCTAAGTAGTCAAGTGACTGTTCACTTAGATTTAAGACATTTCTCTTCTCCTGTCATCAGCATTAGAGCCCAGATACTAAAGAGGCAAAAGTCATGGAAACCACTGGAGGGCCATTATCTTTCCTCATCTCCCCCATGTTGC TCATCACAAAGGTCTGCGGTTGGTAAAGAGAGGAACTGGATGAAAGGGTTTTGATGGGTTGTTCAAATCTACCACCAAGGCTGTAAACAACTCCCTGAATGAAAGCATCATACTTACTCTTCTTCTAGCCTGGATTTAAAAAAGGGGGGAA +A00126:224:H7HMWDSX3:3:1114:27199:9643 Test_seq 171239 Test_seq 171657 + - UU A00126:224:H7HMWDSX3:3:1114:27199:964397Test_seq17123960151M=171507419GTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATGGGATTCGATGAGCATCTTGGATAGATATCCTTTCGTCTTTCATGATGTCAGGGAAGTTTTGTGTCAGGAGTTCTTCAACTATTTTCTCTGF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFF:FFFFNM:i:1MD:Z:63G87MC:Z:151MAS:i:146XS:i:65Yt:Z:UU A00126:224:H7HMWDSX3:3:1114:27199:9643145Test_seq17150760151M=171239-419TTTTTTCAGCTATGTTGCAGTTGATTCCCTGGTCCTCCAGAAGTCCCAGTCTACATTCTAATTGGTCGAGTCTGCTCCTCTGACTTTCTATTGCGTTGTCTAATTCTGTAATTTTATTGTTAATCTTTTGGATTTCTACATGCTGTCTCTCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:3MD:Z:17G46C11T74MC:Z:151MAS:i:136XS:i:47Yt:Z:UU 60 60 GTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATGGGATTCGATGAGCATCTTGGATAGATATCCTTTCGTCTTTCATGATGTCAGGGAAGTTTTGTGTCAGGAGTTCTTCAACTATTTTCTCTG TTTTTTCAGCTATGTTGCAGTTGATTCCCTGGTCCTCCAGAAGTCCCAGTCTACATTCTAATTGGTCGAGTCTGCTCCTCTGACTTTCTATTGCGTTGTCTAATTCTGTAATTTTATTGTTAATCTTTTGGATTTCTACATGCTGTCTCTC +A00126:224:H7HMWDSX3:3:1114:26341:10097 ! 0 Test_seq 169294 - + NR A00126:224:H7HMWDSX3:3:1114:26341:10097145Test_seq16962060127M24S=169294-453TTCATTCTCGTTGCTTTCTATGAATTTCCTTATTCCCTCCTTAATGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTATTGTTCATTTTCCAAGTATTTGATTTCTTTTCCCTAGTTTTTCTGTTATTGATCAATCCTCTTTTCTTACTCFF:FFFFFFFFF:FFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:F:FFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFNM:i:4MD:Z:8T11A21G0G83MC:Z:151MAS:i:107XS:i:72Yt:Z:NR A00126:224:H7HMWDSX3:3:1114:26341:1009797Test_seq16929460151M=169620453TGTTTCCTTTCCTGTATTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFF:FFFFFFFFFF,FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,F,FFFFFFFFNM:i:1MD:Z:15T135MC:Z:127M24SAS:i:146XS:i:107Yt:Z:NR 0 60 TGTTTCCTTTCCTGTATTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTT +A00126:224:H7HMWDSX3:3:1114:18647:10394 ! 0 Test_seq 171664 - - NR A00126:224:H7HMWDSX3:3:1114:18647:10394161Test_seq1712866030S121M=171514379TGTGCAACCATTCTTACCCTCTTTTTCTAAATCAATCTTAAATGGGGTTCGATGAGCATCTTGGATAGATATCCTTTCGTCTTTCATGATGTCAGGGAAGTTTTGTGTCAGGAGTTCTTCAACTATTTTCTCTGTGTTTTCTGTCCCCCCTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFF:FFFF,F,FFFNM:i:1MD:Z:114G6MC:Z:151MAS:i:116XS:i:43Yt:Z:NR A00126:224:H7HMWDSX3:3:1114:18647:1039481Test_seq17151460151M=171286-379AGCTATGTTGGAGTTGATTCCCTGGTCCTCCAGAAGTCCCAGTCTACATTCTAATTGCTCGAGTCTGCTCCTCTGACTTTCTATTGCGTTGTCTAATTCTGTAATTTTATTGTTAATCTTTTGGATTTCTACATGCTGTCTCTCTATGGATFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:69T81MC:Z:30S121MAS:i:146XS:i:56Yt:Z:NR 0 60 AGCTATGTTGGAGTTGATTCCCTGGTCCTCCAGAAGTCCCAGTCTACATTCTAATTGCTCGAGTCTGCTCCTCTGACTTTCTATTGCGTTGTCTAATTCTGTAATTTTATTGTTAATCTTTTGGATTTCTACATGCTGTCTCTCTATGGAT +A00126:224:H7HMWDSX3:3:1114:8006:11130 Test_seq 168738 Test_seq 169111 + - UU A00126:224:H7HMWDSX3:3:1114:8006:1113097Test_seq16873860151M=168961374AAAGCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTCTATTGGCTAGAATTTTGTTGAGGATTTTTGCATCTATGTTCATGAGGGATATAGGTCTAAAATTTTCTTTTTTTGTAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:,FFFF:F::FFF,F:FF,F:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF,FF:FFFFFFF:FFFFFFFF,F,FFFFFFFFFFFFFF,NM:i:1MD:Z:3C147MC:Z:151MAS:i:147XS:i:81Yt:Z:UU A00126:224:H7HMWDSX3:3:1114:8006:11130145Test_seq16896160151M=168738-374TCTATGCTTTGAAATACCTTCAGTAGTAATGGTGTTAAGTCTTCTCTGAAGGTTTGGTAGAACTCTGCAGTGAAGCTGTCTGGGCCAGGGCCTTTTTTTGTTGGAAGTTTTTTGATTACCGTTTCAATCTCTTTTTTTGTTATGGGTCTATFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:4MD:Z:28G47C12A1T59MC:Z:151MAS:i:131XS:i:81Yt:Z:UU 60 60 AAAGCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTCTATTGGCTAGAATTTTGTTGAGGATTTTTGCATCTATGTTCATGAGGGATATAGGTCTAAAATTTTCTTTTTTTGTA TCTATGCTTTGAAATACCTTCAGTAGTAATGGTGTTAAGTCTTCTCTGAAGGTTTGGTAGAACTCTGCAGTGAAGCTGTCTGGGCCAGGGCCTTTTTTTGTTGGAAGTTTTTTGATTACCGTTTCAATCTCTTTTTTTGTTATGGGTCTAT +A00126:224:H7HMWDSX3:3:1114:8223:11193 Test_seq 168738 Test_seq 169111 + - UU A00126:224:H7HMWDSX3:3:1114:8223:1119397Test_seq16873860151M=168961374AAAGCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTCTATTGGCTAGAATTTTGTTGAGGATTTTTGCATCTATGTTCATGAGGGATATAGGTCTAAAATTTTCTTTTTTTGTAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF:FFF:F:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF,FFFF,FFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFNM:i:1MD:Z:3C147MC:Z:151MAS:i:147XS:i:81Yt:Z:UU A00126:224:H7HMWDSX3:3:1114:8223:11193145Test_seq16896160151M=168738-374TCTATGCTTTGAAATACCTTCAGTAGTAATGGTGTTAAGTCTTCTCTGAAGGTTTGGTAGAACTCTGCAGTGAAGCTGTCTGGGCCAGGGCCTTTTTTTGTTGGAAGTTTTTTGATTACCGTTTCAATCTCTTTTTTTGTTATGGGTCTATF,FFFFF:FFFFF:FF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFNM:i:4MD:Z:28G47C12A1T59MC:Z:151MAS:i:131XS:i:81Yt:Z:UU 60 60 AAAGCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTCTATTGGCTAGAATTTTGTTGAGGATTTTTGCATCTATGTTCATGAGGGATATAGGTCTAAAATTTTCTTTTTTTGTA TCTATGCTTTGAAATACCTTCAGTAGTAATGGTGTTAAGTCTTCTCTGAAGGTTTGGTAGAACTCTGCAGTGAAGCTGTCTGGGCCAGGGCCTTTTTTTGTTGGAAGTTTTTTGATTACCGTTTCAATCTCTTTTTTTGTTATGGGTCTAT +A00126:224:H7HMWDSX3:3:1114:21658:14043 ! 0 Test_seq 167949 - + NR A00126:224:H7HMWDSX3:3:1114:21658:14043145Test_seq168149710M1D120M21S=167949-331TTCTTAAGTATTTATCTTCTTGGGGGCTACTGTGAATGGTATTGATTTGGTTATTTCCTCTTCGGTGTTCTTTTTGTTGATGTAGAGGAATCCAAGTGATTTTTGTATGTTTATTTTATAACCTGAGACTGATCTCCTTTTTGATGTCTGGF:FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:4MD:Z:3C6^T9C101T8MC:Z:151MAS:i:110XS:i:105XA:Z:Test_seq,+212759,21S117M1D13M,5;Yt:Z:NR A00126:224:H7HMWDSX3:3:1114:21658:1404397Test_seq16794960151M=168149331AAAATATGACATTGGTATTTGGATTGGAAGTGCGTTATATGTATAGATGGCTTTTGGTAGAATAGACATTTTTACTATGTTAAGTCTTCCTATCCATGAGCAGGGTATGTTTTTCCACTTAAGTATGTCCTTTTGAATTTCTTGTAGCAGAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:111C39MC:Z:10M1D120M21SAS:i:146XS:i:80Yt:Z:NR 0 60 AAAATATGACATTGGTATTTGGATTGGAAGTGCGTTATATGTATAGATGGCTTTTGGTAGAATAGACATTTTTACTATGTTAAGTCTTCCTATCCATGAGCAGGGTATGTTTTTCCACTTAAGTATGTCCTTTTGAATTTCTTGTAGCAGA +A00126:224:H7HMWDSX3:3:1114:9028:14058 Test_seq 169008 Test_seq 169481 + - UU A00126:224:H7HMWDSX3:3:1114:9028:1405897Test_seq16900860146M5S=169331474AAAGGTTTGGTAGAACTCTGCAGTGAAGCCGTCTGGGCCAGGACTTTTTTTTGTTGGAAGTTTTTTGATTACCGTTTCAATCTCTTTTTTTGTTATGGGTCTATTTAGTTGTTCTAGTTCTGAATGTGTTAGTTTAGGTAGGTAGTATTGTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFF,FFFFFFFFF,FFFFFF:FFF,,FFFF,F:F,F,F:F,FFFF,FF:,:,FF,FF:F:FFF:F,FFF,,F,F,F,F,F,:FFF:FFF,FFFFFFFNM:i:2MD:Z:0G115C29MC:Z:151MAS:i:140XS:i:67Yt:Z:UU A00126:224:H7HMWDSX3:3:1114:9028:14058145Test_seq16933160151M=169008-474ATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGGTTCTTTTGTTGCTCF:FFFFFFFF:FFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFF:FF:FFFFFFFFFF,:FFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:F:FFFNM:i:0MD:Z:151MC:Z:146M5SAS:i:151XS:i:111Yt:Z:UU 60 60 AAAGGTTTGGTAGAACTCTGCAGTGAAGCCGTCTGGGCCAGGACTTTTTTTTGTTGGAAGTTTTTTGATTACCGTTTCAATCTCTTTTTTTGTTATGGGTCTATTTAGTTGTTCTAGTTCTGAATGTGTTAGTTTAGGTAGGTAGTATTGT ATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGGTTCTTTTGTTGCTC +A00126:224:H7HMWDSX3:3:1114:20925:15186 Test_seq 168989 Test_seq 169469 + - UU A00126:224:H7HMWDSX3:3:1114:20925:1518697Test_seq16898960151M=169319481GTGGTGTTAACTCTTCTCTGAAAGTTTGGTAGAACTCTGCAGTGAAGCCGTCTGGGCCAGGGCTTTTTTTTGTTGGGAGTTTTTTGATTACCGTTTCAATCTCTTTTTTTGTTATGGGTCTATTTAGTTGTTCTACTTCTGAATGTGTTAGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,,FFFFF,FFFFFFFFFFFFFFFF::::FFFFF,F:FFF,:F:FFFFF,:F,F,FFFF,FFFFFFFFFFFF,F,F,F:FNM:i:4MD:Z:10G11G38A14A74MC:Z:151MAS:i:131XS:i:85Yt:Z:UU A00126:224:H7HMWDSX3:3:1114:20925:15186145Test_seq16931960151M=168989-481TCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGATT:FFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:148G2MC:Z:151MAS:i:148XS:i:112Yt:Z:UU 60 60 GTGGTGTTAACTCTTCTCTGAAAGTTTGGTAGAACTCTGCAGTGAAGCCGTCTGGGCCAGGGCTTTTTTTTGTTGGGAGTTTTTTGATTACCGTTTCAATCTCTTTTTTTGTTATGGGTCTATTTAGTTGTTCTACTTCTGAATGTGTTAG TCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGATT +A00126:224:H7HMWDSX3:3:1114:13684:16986 ! 0 Test_seq 209433 - + NU A00126:224:H7HMWDSX3:3:1114:13684:16986133Test_seq2094330*=2094330GCAAGGATGGTGAGACCGCATCTTACATACTTTGGTCATGTTGTCAGGAGGGAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFMC:Z:53MAS:i:0XS:i:0Yt:Z:NU A00126:224:H7HMWDSX3:3:1114:13684:1698673Test_seq2094331153M=2094330TCCCTCCTGACAACATGTCCAAAGTATGTAAGACGCAGCCTCGCCATCCTTGCFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFF,FF,F,FFF,FFFFFFFFFFNM:i:1MD:Z:38T14AS:i:48XS:i:43XA:Z:Test_seq,-240774,53M,2;Test_seq,+332659,53M,2;Yt:Z:NU 0 11 GCAAGGATGGTGAGACCGCATCTTACATACTTTGGTCATGTTGTCAGGAGGGA TCCCTCCTGACAACATGTCCAAAGTATGTAAGACGCAGCCTCGCCATCCTTGC +A00126:224:H7HMWDSX3:3:1114:4372:17425 Test_seq 168659 Test_seq 212761 - - UU A00126:224:H7HMWDSX3:3:1114:4372:17425113Test_seq16850960151M=21262544103ATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTGAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCFFF:FFFFF:FFFFFFF:FFFF:FFFFFFFFF:FFFFFFFFFF,,FFFFFFFFFFFF:FFFFFFFF::FFFFFFFFFFFFFFFFFFF,FFF::FFFFFFFF:FFFFFFFFFFFFFF,FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF:NM:i:1MD:Z:100A50MC:Z:137M14SAS:i:146XS:i:83Yt:Z:UU A00126:224:H7HMWDSX3:3:1114:4372:17425177Test_seq21262529137M14S=168509-44103AAAGAAATAAAGGGTATCCGGATTGGCAAGGAGGAAGTAAAGTTATCACTATTTGCAGATGACATGATCTTATACACAGAAAACCCTAAGGAATCCTCCAGAAAACTACTGAAACTAATAGAAGAGTTTGGCAGAGTGATCACATGACAGAFF,FFF:FFFFFF:F:FFFFFF:FFFFF,FFF:FFFFFFFF:FF,FFF:FF:FFF:,FFFF:FFFFF:FFFFFFFFF:FFF::,FFF:F,,FFFFFF,FF,FF:FF::FFFF,,FFFFFFF:FFFFFF:FFFFFFFFFFF:F:,F:F:,FFNM:i:5MD:Z:14C3T22A5T39T49MC:Z:151MAS:i:112XS:i:97XA:Z:Test_seq,+168277,14S137M,8;Yt:Z:UU 60 29 ATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTGAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGC AAAGAAATAAAGGGTATCCGGATTGGCAAGGAGGAAGTAAAGTTATCACTATTTGCAGATGACATGATCTTATACACAGAAAACCCTAAGGAATCCTCCAGAAAACTACTGAAACTAATAGAAGAGTTTGGCAGAGTGATCACATGACAGA +A00126:224:H7HMWDSX3:3:1114:6126:18145 ! 0 Test_seq 383221 - + NR A00126:224:H7HMWDSX3:3:1114:6126:1814581Test_seq38336760126M25S=383221-272TAAAGTATTATGACATGTGCAAAGAGCTGGAGATGGAAAACCAAAAGGGAAGAACATGCTTGGCATTTCTCCAGCTGAAAGAACTGAAGAAAAAGTTGAAGCTTCGAGTTGCAATAGTGAAGGATTTAAAACATTACCTGCAAAGGTAAAGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:126MC:Z:151MAS:i:126XS:i:0Yt:Z:NR A00126:224:H7HMWDSX3:3:1114:6126:18145161Test_seq38322160151M=383367272ACAGGAAAGAATGAAAAGACCAAGATGGATGTCAGAGGAGACTCTGAAACTTGCTCTCTAATGTTGAGCCGCTAAAGCAAAAGGAAGAATTGATGAAGTAAAGGAACTGAACAGAAGATTTCAAAAGGCAGCTCGAGAAGACAAAGTAAAGFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:,FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:126M25SAS:i:151XS:i:79Yt:Z:NR 0 60 ACAGGAAAGAATGAAAAGACCAAGATGGATGTCAGAGGAGACTCTGAAACTTGCTCTCTAATGTTGAGCCGCTAAAGCAAAAGGAAGAATTGATGAAGTAAAGGAACTGAACAGAAGATTTCAAAAGGCAGCTCGAGAAGACAAAGTAAAG +A00126:224:H7HMWDSX3:3:1114:31195:18411 ! 0 Test_seq 168601 - - NR A00126:224:H7HMWDSX3:3:1114:31195:1841197Test_seq1682381722S129M=168451364CTAGCAGTGGTGTCCCTTGATCAATCCAAGTGATTTTTGTATGGTTATTTTATAACCTGAGACTCTTCCAGACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGGTTTTCCGTGTATACGATCATGTCATCTGCAAATAGTFFFFFFFFFFFFFFFF,,FFFFFFFFFFFFFFFF:FFFF:FFF,FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFNM:i:6MD:Z:21T11T14A50A8G18A1MC:Z:151MAS:i:102XS:i:93XA:Z:Test_seq,-212672,129M22S,8;Yt:Z:NR A00126:224:H7HMWDSX3:3:1114:31195:18411145Test_seq16845160151M=168238-364ATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCTGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTA:FFFFFFFF,FFFFFFFFFFFFF,FFFFFFFF,FFFFFFFF,FFFF,FFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FF:FF:FFFFFFFFFFFFFFFFF,FFFFFNM:i:0MD:Z:151MC:Z:22S129MAS:i:151XS:i:87Yt:Z:NR 0 60 ATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCTGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTA +A00126:224:H7HMWDSX3:3:1114:30834:18724 Test_seq 169313 Test_seq 169608 + - UU A00126:224:H7HMWDSX3:3:1114:30834:18724161Test_seq16931360151M=169458296CTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACAAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGAF:FFFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:F,FFFFFFFFNM:i:1MD:Z:53C97MC:Z:151MAS:i:146XS:i:107Yt:Z:UU A00126:224:H7HMWDSX3:3:1114:30834:1872481Test_seq16945860151M=169313-296GCCTGATGGGTTCTTTTGTTGCTCACTTTCTATTTGTTCAAGTTGTAGGGACAGTTCTCTGATTTTGGCTCTTTCTTCTTTTTGTATGTGTACATTTATCAATATAAATTGGCCTCTGAGCACTGCTTTTGCTGTGTCCCAGAGGTTTTGAFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:6MD:Z:46C0A13C0C28G8G50MC:Z:151MAS:i:121XS:i:71Yt:Z:UU 60 60 CTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACAAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGA GCCTGATGGGTTCTTTTGTTGCTCACTTTCTATTTGTTCAAGTTGTAGGGACAGTTCTCTGATTTTGGCTCTTTCTTCTTTTTGTATGTGTACATTTATCAATATAAATTGGCCTCTGAGCACTGCTTTTGCTGTGTCCCAGAGGTTTTGA +A00126:224:H7HMWDSX3:3:1114:23339:18771 Test_seq 167331 Test_seq 167571 + - UU A00126:224:H7HMWDSX3:3:1114:23339:18771161Test_seq16733123151M=167439241TGAGATGTCATAGCTGAATATTCTTTCCCAGTCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFF:FFFFFF:FFFFFFFFFFFFFF:F,FFFFFFFFF:FFFFFFFFFFF:FFFFF::F,F,FFFFF,:F:FFNM:i:1MD:Z:37A113MC:Z:133M18SAS:i:146XS:i:135XA:Z:Test_seq,-213553,151M,4;Yt:Z:UU A00126:224:H7HMWDSX3:3:1114:23339:1877181Test_seq16743960133M18S=167331-241ATCTGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATGCCCTGTATTAGGGCTCCTAGGGTAGATCCTATTTTTTCTTCCATGATTTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCGATCCAAGTAAAATGAAAFFF,FFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:2MD:Z:72T23C36MC:Z:151MAS:i:123XS:i:85Yt:Z:UU 23 60 TGAGATGTCATAGCTGAATATTCTTTCCCAGTCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTG ATCTGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATGCCCTGTATTAGGGCTCCTAGGGTAGATCCTATTTTTTCTTCCATGATTTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCGATCCAAGTAAAATGAAA +A00126:224:H7HMWDSX3:3:1114:22806:19194 Test_seq 165892 Test_seq 166206 + - UU A00126:224:H7HMWDSX3:3:1114:22806:19194161Test_seq1658926010M2D141M=166056315GCATCTCTCTCCCCTCCACTGACCAGTCCTCTCCATGCCTGATGATTTGTCTTCGGGGATGGTTCCTGTCTTGTGCCATCAGAAGTTCTGGGGAGCATTGTCTCTAGGATTCCTCTAGTCGCAGTCATACCATTATGTATGGTCTTTTCATFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFF:FFFFFFFF:FFFFFF:FFFFFFFFFFFFF:FFFF,FFFFFF:FFFF,:FFFFFFFFFFFFF,,FFFFFFFFFFFFFFFFFFF:FFFFFFFF:FFFFF,FF:FFFFFFFFF:FFF,NM:i:15MD:Z:10^CC4C2G12T1T4T7G24C34G14A2T5T5G12T2MC:Z:151MAS:i:80XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1114:22806:1919481Test_seq16605660151M=165892-315GTCTGTATCCCATTGGTCTCCTGCTCCCTCAGGAGTTGTCTGTTGTGCTCCCTGACAGGGCAGACATCGATTGTGGCCAGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGFFFFFFFFFFFFFFFFFF:FFFFFF,FFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFFFFFF:F,FFF:FFFFFFFFFFFFFF:::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFNM:i:1MD:Z:78G72MC:Z:10M2D141MAS:i:146XS:i:76Yt:Z:UU 60 60 GCATCTCTCTCCCCTCCACTGACCAGTCCTCTCCATGCCTGATGATTTGTCTTCGGGGATGGTTCCTGTCTTGTGCCATCAGAAGTTCTGGGGAGCATTGTCTCTAGGATTCCTCTAGTCGCAGTCATACCATTATGTATGGTCTTTTCAT GTCTGTATCCCATTGGTCTCCTGCTCCCTCAGGAGTTGTCTGTTGTGCTCCCTGACAGGGCAGACATCGATTGTGGCCAGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGG +A00126:224:H7HMWDSX3:3:1114:24243:19304 Test_seq 73194 Test_seq 92375 + - UR A00126:224:H7HMWDSX3:3:1114:24243:1930497Test_seq731946032M119S=9222519182AAAGGAAGAGCATTCATTCAGAGGACATTTAAGTCACCCTTAACGTCCATAATTCTACCAACTGCCTCTTCGAGGCAGTCTAGGCTTTGACTATCGAGTGCATCGAACCCCTCCAGCCTCTACCCATTATCCAATTCCAAAACCACTTCCAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFNM:i:0MD:Z:32MC:Z:151MAS:i:32XS:i:0SA:Z:Test_seq,92047,+,31S120M,60,0;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1114:24243:193042145Test_seq920476031H120M=92225329AGTCACCCTTAACGTCCATAATTCTACCAACTGCCTCTTCGAGGCAGTCTAGGCTTTGACTATCGAGTGCATCGAACCCCTCCAGCCTCTACCCATTATCCAATTCCAAAACCACTTCCAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFNM:i:0MD:Z:120MC:Z:151MAS:i:120XS:i:0SA:Z:Test_seq,73194,+,32M119S,60,0;Yt:Z:UR A00126:224:H7HMWDSX3:3:1114:24243:19304145Test_seq9222560151M=73194-19182CCACCTAGTGCTGCCATAACAAAAGATACCACAAGGGTGTAGCTTTAAAGAACAGAAATTGGTTTTCTCACAGGCCAGGCCAAGCCCGTTGCCATCAAGCTGATGCCCACACGTGGTGACCCTGCGTGTGTCGGAGGAGAACTGTGCCCCCFFFFFFFFFFF:FFFF,,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:32M119SAS:i:151XS:i:0Yt:Z:UR 60 60 AAAGGAAGAGCATTCATTCAGAGGACATTTAAGTCACCCTTAACGTCCATAATTCTACCAACTGCCTCTTCGAGGCAGTCTAGGCTTTGACTATCGAGTGCATCGAACCCCTCCAGCCTCTACCCATTATCCAATTCCAAAACCACTTCCA CCACCTAGTGCTGCCATAACAAAAGATACCACAAGGGTGTAGCTTTAAAGAACAGAAATTGGTTTTCTCACAGGCCAGGCCAAGCCCGTTGCCATCAAGCTGATGCCCACACGTGGTGACCCTGCGTGTGTCGGAGGAGAACTGTGCCCCC +A00126:224:H7HMWDSX3:3:1114:19633:20400 Test_seq 167895 Test_seq 168166 + - UU A00126:224:H7HMWDSX3:3:1114:19633:20400161Test_seq16789560151M=168016272CCGGGGCTTCTTTCCCTTCCATATGAAATTAGTGATTTGTTTCTCTATCCCCTTAAAATATGACATTGGTATTTGGATTGGAAGTGCGTTATATGTATAGATGGCTTTTGGTAGAATAGACATTTTTACTATGTTAAGTCTTCCTATCCATFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFNM:i:1MD:Z:6G144MC:Z:151MAS:i:146XS:i:67Yt:Z:UU A00126:224:H7HMWDSX3:3:1114:19633:2040081Test_seq16801656151M=167895-272ATTTTTACTATGTTAAGTCTTCCTATCCATGAGCAGGGTATGTTTTTCCACTTAAGTATGTCCTTTTGAATTTCTTGTAGCAGAGTTTTTTAGTTTTCTTTGTATAGGTCTTTTACATCCTTGGTAAGATTTATTCCTAAGTATTTTATCT::FF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:5MD:Z:44C44A13G12C5C28MC:Z:151MAS:i:126XS:i:96Yt:Z:UU 60 56 CCGGGGCTTCTTTCCCTTCCATATGAAATTAGTGATTTGTTTCTCTATCCCCTTAAAATATGACATTGGTATTTGGATTGGAAGTGCGTTATATGTATAGATGGCTTTTGGTAGAATAGACATTTTTACTATGTTAAGTCTTCCTATCCAT ATTTTTACTATGTTAAGTCTTCCTATCCATGAGCAGGGTATGTTTTTCCACTTAAGTATGTCCTTTTGAATTTCTTGTAGCAGAGTTTTTTAGTTTTCTTTGTATAGGTCTTTTACATCCTTGGTAAGATTTATTCCTAAGTATTTTATCT +A00126:224:H7HMWDSX3:3:1114:26802:21355 Test_seq 170234 Test_seq 170573 + - UU A00126:224:H7HMWDSX3:3:1114:26802:21355161Test_seq17023460151M=170423340TTGCTACTCCTCTTCTTTTTTGCTTATTGTTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCGTGACTCTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFF:FFFFFF:FFF:F:FFFFFFFFNM:i:1MD:Z:28A122MC:Z:151MAS:i:146XS:i:78Yt:Z:UU A00126:224:H7HMWDSX3:3:1114:26802:2135581Test_seq17042360151M=170234-340GCGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTTTTCATGTGTGTTGTTGACAATTTCATTTTTCCACATACTTTTTTGTGCTGAGGCGTTTTTCTTAGTAAATTGTGAGATCCTCATTTTCATAGTGTTTGACTTTFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:7MD:Z:64G1C0T32A14G23A5A5MC:Z:151MAS:i:116XS:i:48Yt:Z:UU 60 60 TTGCTACTCCTCTTCTTTTTTGCTTATTGTTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCGTGACTCT GCGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTTTTCATGTGTGTTGTTGACAATTTCATTTTTCCACATACTTTTTTGTGCTGAGGCGTTTTTCTTAGTAAATTGTGAGATCCTCATTTTCATAGTGTTTGACTTT +A00126:224:H7HMWDSX3:3:1114:9480:22169 Test_seq 212718 Test_seq 213006 + - UU A00126:224:H7HMWDSX3:3:1114:9480:22169161Test_seq21271852151M=212860289TCCTCCAGAAAACTACTGAAACTAATAGAAGAGTTTGGCAGAGTCTCAGGTTATAAGATAAACATACAAAAATCACTTGGATTCCTCTACATCAACAAAAAGAACATCAAAGAGGAAATCACCAAATCAATACCATTCACAGTAGCCCCCAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:119A31MC:Z:16M1I9M3I122MAS:i:146XS:i:121XA:Z:Test_seq,-168170,151M,6;Yt:Z:UU A00126:224:H7HMWDSX3:3:1114:9480:2216981Test_seq2128606016M1I9M3I122M=212718-289TAGCCCCCAAGAAGATAAAAATACTTAGGAGGAATAAATCTTACCAAAGACATAAAAGACCTATACGAAGAAAACTACAATGTACTACTGCAAGAAACTAAAAAGGACCTACTTAAGTGGAAAAACATACCTTGCTCATGGATAGGAAGAC:FFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFF:FFFFF:FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFNM:i:10MD:Z:46T0G14A7T5A8A61MC:Z:151MAS:i:101XS:i:44Yt:Z:UU 52 60 TCCTCCAGAAAACTACTGAAACTAATAGAAGAGTTTGGCAGAGTCTCAGGTTATAAGATAAACATACAAAAATCACTTGGATTCCTCTACATCAACAAAAAGAACATCAAAGAGGAAATCACCAAATCAATACCATTCACAGTAGCCCCCA TAGCCCCCAAGAAGATAAAAATACTTAGGAGGAATAAATCTTACCAAAGACATAAAAGACCTATACGAAGAAAACTACAATGTACTACTGCAAGAAACTAAAAAGGACCTACTTAAGTGGAAAAACATACCTTGCTCATGGATAGGAAGAC +A00126:224:H7HMWDSX3:3:1114:8594:22639 Test_seq 214119 Test_seq 214168 + - UU A00126:224:H7HMWDSX3:3:1114:8594:22639161Test_seq2141192450M=21411950CAAGAGCCTTCATACAAACAGATATATGCACACCCATGTTTATGGCAGCTFFF::FFFFFFFFFF:FFFFFFFFFFF:FFFFFF:FF:FFFFF:FFF::FNM:i:3MD:Z:0T11C30T6MC:Z:50MAS:i:39XS:i:31Yt:Z:UU A00126:224:H7HMWDSX3:3:1114:8594:2263981Test_seq2141191150M=214119-50CAAGAGCCTTCACACAAACAGATATATGCACACCCATGTTTATTGCAGCT:,FFFFF,FFFF,F,:FF,F,,FF:FF:FFFF:,FF,FFF:F:F:FFF,FNM:i:1MD:Z:0T49MC:Z:50MAS:i:49XS:i:45XA:Z:Test_seq,+166866,50M,1;Yt:Z:UU 24 11 CAAGAGCCTTCATACAAACAGATATATGCACACCCATGTTTATGGCAGCT CAAGAGCCTTCACACAAACAGATATATGCACACCCATGTTTATTGCAGCT +A00126:224:H7HMWDSX3:3:1114:9335:22952 ! 0 Test_seq 171244 - + NR A00126:224:H7HMWDSX3:3:1114:9335:2295281Test_seq17153960127M24S=171244-422CCCTCCGGAAGTCCCAGTCTACATTCTAATTGCTCGAGTCTGCTCCTCTGGCTTTCTATTGCGTTGTCAAATTCTGTAATTTTATTGTTAATCTTTTGGATTTCTACATGCTGTCTCTCTATGGATTACTCAGGCTAAGATTTTTGGTATTFFF:FFF,FFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:5MD:Z:0T5A37T5A17T58MC:Z:151MAS:i:106XS:i:47Yt:Z:NR A00126:224:H7HMWDSX3:3:1114:9335:22952161Test_seq17124460151M=171539422GGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATGGGGTTCGATGAGCATCTTGGATAGATATCCTTTCGTCTTTCATGATGTCAGGGAAGTTTTGTGTCAGGAGTTCTTCAACTATTTTCTCTGTGTTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:FF,FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,F:FFFF,FFFFFFFFF:FNM:i:0MD:Z:151MC:Z:127M24SAS:i:151XS:i:66Yt:Z:NR 0 60 GGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATGGGGTTCGATGAGCATCTTGGATAGATATCCTTTCGTCTTTCATGATGTCAGGGAAGTTTTGTGTCAGGAGTTCTTCAACTATTTTCTCTGTGTTT +A00126:224:H7HMWDSX3:3:1114:30264:23156 Test_seq 166091 Test_seq 166563 + - UU A00126:224:H7HMWDSX3:3:1114:30264:2315697Test_seq16609160151M=166413473TTGTCTGTTGTGTTCCCTGACAGGGCAGACATCGATTGTGGCCGGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCAAGTGGCCCTTTCTGTCTCTTGGGTTCTTAGTTGTCGTGTGACCTTGGTGTTCTTCCTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FF,:FFF:F:,FFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFNM:i:3MD:Z:12C80T39G17MC:Z:151MAS:i:136XS:i:75Yt:Z:UU A00126:224:H7HMWDSX3:3:1114:30264:23156145Test_seq16641360151M=166091-473CCCCTGCTCCGCTGACCTTTGAAGCTTTCATTTTATCCCGGAAACCTCTTTGCTTTTAGTCCAGTCCAATTAGGCTGACCTTCCTTGTATTGTGTGTTGTCTTTCCCTTCACCCAAAGCAGTTCTTATCTACTGATTGATCAATAAAAAACFFFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:30Yt:Z:UU 60 60 TTGTCTGTTGTGTTCCCTGACAGGGCAGACATCGATTGTGGCCGGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCAAGTGGCCCTTTCTGTCTCTTGGGTTCTTAGTTGTCGTGTGACCTTGGTGTTCTTCCTT CCCCTGCTCCGCTGACCTTTGAAGCTTTCATTTTATCCCGGAAACCTCTTTGCTTTTAGTCCAGTCCAATTAGGCTGACCTTCCTTGTATTGTGTGTTGTCTTTCCCTTCACCCAAAGCAGTTCTTATCTACTGATTGATCAATAAAAAAC +A00126:224:H7HMWDSX3:3:1114:26386:26052 Test_seq 336841 Test_seq 337219 + - UU A00126:224:H7HMWDSX3:3:1114:26386:2605297Test_seq33684160151M=337069379GTTACACTCTGTAACACATGGGGTTGCCATGAGTCAGAATCTACTCAATGGCAATGGGTTTTTTGTTTTTGGTTTACCTCTTAGGGTTGTTATCAGGATTAAACAAACTAATACTATATTTGGAAGAGTACCTGGTGTGTATAATAACTGTFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1114:26386:26052145Test_seq33706960151M=336841-379TTGTTAGTATGAATGTATGCACCTTAGACCCTCAAAAGAGAGAACGACTGTCTCATAGATGTCTACTTAGTCAAGTGTATTGCTAGTGTTGTCCTGGGCGATGTTTGAAACCATAGTGCTTCAGGTCGAGGCTGTTAATTCATTCTTGATCFFFFF,:FFF:FFFFFF:FFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU 60 60 GTTACACTCTGTAACACATGGGGTTGCCATGAGTCAGAATCTACTCAATGGCAATGGGTTTTTTGTTTTTGGTTTACCTCTTAGGGTTGTTATCAGGATTAAACAAACTAATACTATATTTGGAAGAGTACCTGGTGTGTATAATAACTGT TTGTTAGTATGAATGTATGCACCTTAGACCCTCAAAAGAGAGAACGACTGTCTCATAGATGTCTACTTAGTCAAGTGTATTGCTAGTGTTGTCCTGGGCGATGTTTGAAACCATAGTGCTTCAGGTCGAGGCTGTTAATTCATTCTTGATC +A00126:224:H7HMWDSX3:3:1114:6424:26209 Test_seq 166060 Test_seq 166536 + - UU A00126:224:H7HMWDSX3:3:1114:6424:2620997Test_seq16606060151M=166386477GTATCCCATTGGTCTCCTGCTCCCTCAGGAGTTGTCTGTTGTGCTCCCTGACAGGGCAGACATCGATTGTGGCCGGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF,F:FFFF,FFFFFFFFF:FNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:57Yt:Z:UU A00126:224:H7HMWDSX3:3:1114:6424:26209145Test_seq16638660151M=166060-477CCTCAAACCAAGGTCCCCGGACCTCAGCCCCTGCTCCGCTGACCTTTGAAGCTTTCATTTTATCCCGGAAACCTCTTTGCCTTTAATCCAGTCCAATTAGGCTGACCTTCCTTGTATTGAGTGTTGTCTTTCCCTTCACCCAAAGCAGTTCFFFFFFFFF:F:,FFFFF:FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:7MD:Z:10T1T5A4C56T4G33T31MC:Z:151MAS:i:116XS:i:32Yt:Z:UU 60 60 GTATCCCATTGGTCTCCTGCTCCCTCAGGAGTTGTCTGTTGTGCTCCCTGACAGGGCAGACATCGATTGTGGCCGGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCT CCTCAAACCAAGGTCCCCGGACCTCAGCCCCTGCTCCGCTGACCTTTGAAGCTTTCATTTTATCCCGGAAACCTCTTTGCCTTTAATCCAGTCCAATTAGGCTGACCTTCCTTGTATTGAGTGTTGTCTTTCCCTTCACCCAAAGCAGTTC +A00126:224:H7HMWDSX3:3:1114:14534:27164 Test_seq 153478 Test_seq 153531 + - UU A00126:224:H7HMWDSX3:3:1114:14534:27164161Test_seq1534781354M=15347854TCTAGGTTTTCAAATTTGGTAGAGTACAATTTTTCATAGTAATCTGATATGATTFFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:18T35MC:Z:54MAS:i:49XS:i:44XA:Z:Test_seq,+51125,54M,2;Yt:Z:UU A00126:224:H7HMWDSX3:3:1114:14534:2716481Test_seq1534781254M=153478-54TCTAGGTTTTCAAATTTGTTAGAGTACAATTTTTCATAATAATCTGAAATGATTFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:2MD:Z:38G8T6MC:Z:54MAS:i:44XS:i:39XA:Z:Test_seq,-51125,54M,3;Yt:Z:UU 13 12 TCTAGGTTTTCAAATTTGGTAGAGTACAATTTTTCATAGTAATCTGATATGATT TCTAGGTTTTCAAATTTGTTAGAGTACAATTTTTCATAATAATCTGAAATGATT +A00126:224:H7HMWDSX3:3:1114:23936:27602 Test_seq 169575 Test_seq 169995 + - UU A00126:224:H7HMWDSX3:3:1114:23936:27602161Test_seq16957560151M=169843421GAGCACTGCTTTTGCTGTGTCCCAGAGGTTTTGATAGGAAGTATTTTCATTCTTGTTGCTTTCTAAGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTATTGTTCATTTTCCAAGTATTTGATTTCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:,FFFFFFFFFFFFFFFFFFFFFFFFF:NM:i:1MD:Z:88G62MC:Z:34M1D35M1D82MAS:i:146XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1114:23936:2760281Test_seq1698436034M1D35M1D82M=169575-421TGGTCTATTCTAGAGAATGTTCCATGTGCACTAGAAAAAAAGTATATTTTGCAGCAGTTGGGTGGAGAGTCTGTATAAGTCAATGAGGTCAAGTTGGTTGATTGTTGTAAGTAGGTCTTCCGTGTCTCTATTGAGCTTCTTACTGGATGTCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFNM:i:5MD:Z:23C10^A35^T12T47G21MC:Z:151MAS:i:122XS:i:47Yt:Z:UU 60 60 GAGCACTGCTTTTGCTGTGTCCCAGAGGTTTTGATAGGAAGTATTTTCATTCTTGTTGCTTTCTAAGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTATTGTTCATTTTCCAAGTATTTGATTTC TGGTCTATTCTAGAGAATGTTCCATGTGCACTAGAAAAAAAGTATATTTTGCAGCAGTTGGGTGGAGAGTCTGTATAAGTCAATGAGGTCAAGTTGGTTGATTGTTGTAAGTAGGTCTTCCGTGTCTCTATTGAGCTTCTTACTGGATGTC +A00126:224:H7HMWDSX3:3:1114:5864:28119 ! 0 Test_seq 167568 - + NR A00126:224:H7HMWDSX3:3:1114:5864:28119145Test_seq16790260128M23S=167568-462TTCTTTCCCTTCCATATGAAATTAGTGATTTGTTTCTCTATCCCCTTAAAATATGACATTGGTATTTGGATTGGAAGTGCGTTATATGTATAGATGGCTTTTGGTAGAATAGACATTTTTACTATGTTGATCATTCAAAAGTGGTTGCAGCFFFFFFFF::FFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:128MC:Z:151MAS:i:128XS:i:0Yt:Z:NR A00126:224:H7HMWDSX3:3:1114:5864:2811997Test_seq16756831151M=167902462GATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATCATTTCCCCAATTGACTGACACTGGTCCTTTGTCAAAAATCAGCFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:FFFFFF::F:FFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFF::NM:i:1MD:Z:144T6MC:Z:128M23SAS:i:146XS:i:131XA:Z:Test_seq,-213320,140M2I9M,4;Yt:Z:NR 0 31 GATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATCATTTCCCCAATTGACTGACACTGGTCCTTTGTCAAAAATCAGC +A00126:224:H7HMWDSX3:3:1114:28212:28808 Test_seq 168997 Test_seq 169468 + - UU A00126:224:H7HMWDSX3:3:1114:28212:2880897Test_seq16899760151M=169318472AACTCTTCTCTGAAAATTTGGTAGAACTCTGCAGTGAAGCCGTCCGGGCCAGGACTTTTTTTTGTTGGGAGTTTTTTGATTACCGTTTCAATCTCTTTTTTTGTTATGGGTCTATTTAGTTGTTCTGCTTCTGAATGTGTTAGTTTAGGTAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:F:FFFF:FFFFF::FFFFFFF:FFF,FFFFFF,FFFF:FFFFFFFFF:FFFFF,FF::FFF:FFFF:FFFF::FF:FFFFFFFFF::FFFF:FFFFFFFF:FF,,FF:FFF:NM:i:6MD:Z:2G11G0G28T23A57A24MC:Z:151MAS:i:123XS:i:65Yt:Z:UU A00126:224:H7HMWDSX3:3:1114:28212:28808145Test_seq16931860151M=168997-472GTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGATFFFFFFFFFFFF,FF:FFFFFFFF:FFFFFF:FFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFF,FFFFFFF:FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:149G1MC:Z:151MAS:i:149XS:i:112Yt:Z:UU 60 60 AACTCTTCTCTGAAAATTTGGTAGAACTCTGCAGTGAAGCCGTCCGGGCCAGGACTTTTTTTTGTTGGGAGTTTTTTGATTACCGTTTCAATCTCTTTTTTTGTTATGGGTCTATTTAGTTGTTCTGCTTCTGAATGTGTTAGTTTAGGTA GTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGAT +A00126:224:H7HMWDSX3:3:1114:10176:29168 Test_seq 161012 Test_seq 161354 + - UU A00126:224:H7HMWDSX3:3:1114:10176:29168161Test_seq16101260151M=161204343TTGGGAGCCCCTCTGTTCCCTGTCTTACTGAGTCCCTAGGTTCTATACCCAACCCCCTGTTACCTCCTTGATAGTATAGCCTATCTCTCTTTTACTTGCTTCTCCTCTAACCACACCAGTCTCTTTGCTGTTCCTCAACAGGTGCCCTCCGFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFF:FF:FFFFFF,F::FFFFFFFFFF:FF:FFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:51Yt:Z:UU A00126:224:H7HMWDSX3:3:1114:10176:2916881Test_seq16120460151M=161012-343ACATACAGCTAACTTACCACACACCAGAGAGGCCCGTGGGCCACCTCAGGGGCTGGAACCCCCTGCAGTGACACACGGCATCTTAAATGCATGTGCGTGTTTTCTGTAAAGGATCCACAGCATTCGTCTGATTCTCAAAGGAGCCTGTGAT:,FF::F:FFFFF:FFFF:FFF:FFFFFFFFFFFFFFF:,FF::FF:FFFFFF:,FFFFF:FFFFF,F:FFFFFFFFF:FFFFFFFFF:FF::F:FFFFFFFFFFFFFF:FFFFFFFF,,:FFF,FFFFFFFFFFFFFFF::FFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU 60 60 TTGGGAGCCCCTCTGTTCCCTGTCTTACTGAGTCCCTAGGTTCTATACCCAACCCCCTGTTACCTCCTTGATAGTATAGCCTATCTCTCTTTTACTTGCTTCTCCTCTAACCACACCAGTCTCTTTGCTGTTCCTCAACAGGTGCCCTCCG ACATACAGCTAACTTACCACACACCAGAGAGGCCCGTGGGCCACCTCAGGGGCTGGAACCCCCTGCAGTGACACACGGCATCTTAAATGCATGTGCGTGTTTTCTGTAAAGGATCCACAGCATTCGTCTGATTCTCAAAGGAGCCTGTGAT +A00126:224:H7HMWDSX3:3:1114:28266:30436 Test_seq 168757 Test_seq 169067 + - UU A00126:224:H7HMWDSX3:3:1114:28266:3043697Test_seq16875760151M=168917311GGTATAAATCCCACTTGATCGGGGTGAATTATTTTTTTGATGTGTTGTTGGATTCTATTGGCTAGAATTTTGTTGAGGATTTTTGCATCTATGTTCATGAGGGATATAGGTCTAAAATTTTCTTTTTTTGTAATGTCTTTACCTGGTTTTGFF:FFFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFF,FFF,:FFFFFFFFFF,FFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:FFFNM:i:1MD:Z:20A130MC:Z:151MAS:i:146XS:i:82Yt:Z:UU A00126:224:H7HMWDSX3:3:1114:28266:30436145Test_seq16891760151M=168757-311AGATGGTAGCTTCATAGAAGGAGTTGGGTAGTATTCCGTCTTTTTCTATGCTTTGAAATACCTTCAGTAGTAGTGGTGTTAAGTCTTCTCTAAAGGTTTGGTAGAACTCTGCAGTGAAGCCATCTGGGCCAGGACTTTTTTTTGTTGGAAGFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:5MD:Z:7G11T22G48G29G29MC:Z:151MAS:i:126XS:i:67Yt:Z:UU 60 60 GGTATAAATCCCACTTGATCGGGGTGAATTATTTTTTTGATGTGTTGTTGGATTCTATTGGCTAGAATTTTGTTGAGGATTTTTGCATCTATGTTCATGAGGGATATAGGTCTAAAATTTTCTTTTTTTGTAATGTCTTTACCTGGTTTTG AGATGGTAGCTTCATAGAAGGAGTTGGGTAGTATTCCGTCTTTTTCTATGCTTTGAAATACCTTCAGTAGTAGTGGTGTTAAGTCTTCTCTAAAGGTTTGGTAGAACTCTGCAGTGAAGCCATCTGGGCCAGGACTTTTTTTTGTTGGAAG +A00126:224:H7HMWDSX3:3:1114:7075:30906 Test_seq 168547 Test_seq 168898 + - UU A00126:224:H7HMWDSX3:3:1114:7075:30906161Test_seq16854760151M=168748352GTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATCATGTGGTTTTTGTCTTTTGTTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFF:FFFNM:i:1MD:Z:140A10MC:Z:151MAS:i:146XS:i:86Yt:Z:UU A00126:224:H7HMWDSX3:3:1114:7075:3090681Test_seq16874860151M=168547-352TGCATACCTGGTACAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTCTATTGGCTAGAATTTTGTTGAGGATTTTTGCATCTATGTTCATGAGGGATATAGGTCTAAAATTTTCTTTTTTTGTAATGTCTTTACFFFFFFFFFFFF:FFFFFF::FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFNM:i:1MD:Z:13T137MC:Z:151MAS:i:146XS:i:81Yt:Z:UU 60 60 GTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATCATGTGGTTTTTGTCTTTTGTTT TGCATACCTGGTACAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTCTATTGGCTAGAATTTTGTTGAGGATTTTTGCATCTATGTTCATGAGGGATATAGGTCTAAAATTTTCTTTTTTTGTAATGTCTTTAC +A00126:224:H7HMWDSX3:3:1114:21838:31955 Test_seq 165871 Test_seq 166282 + - UU A00126:224:H7HMWDSX3:3:1114:21838:3195597Test_seq1658716031M3D105M15S=166132412TAATTAGCTCACTCTTCATCAGCATCTCTCTCCCCCCACTGACCAGTCCTTTTCATGCCTGATGATTTGTCTTCGGGGATGGTTCCTGTCCTGTGCCATCAGAAGTTCTGGGGAGCATTGTCTCTGGGATTCCTCTGGTCGCAGTCATACCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:7MD:Z:5G25^CCC6G19T7G70MC:Z:151MAS:i:107XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1114:21838:31955145Test_seq16613260151M=165871-412CCGGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCTTAGTTGTCGTGTGACCTTGGTGTTCTTCCTTTGCCTTTGCTCCAGGTGGGTTGAGACCAATTGATGTATCTTFFFFFFFFFF,F:FF:FF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF,FFFFFFFFFFFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:92G58MC:Z:31M3D105M15SAS:i:146XS:i:91Yt:Z:UU 60 60 TAATTAGCTCACTCTTCATCAGCATCTCTCTCCCCCCACTGACCAGTCCTTTTCATGCCTGATGATTTGTCTTCGGGGATGGTTCCTGTCCTGTGCCATCAGAAGTTCTGGGGAGCATTGTCTCTGGGATTCCTCTGGTCGCAGTCATACC CCGGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCTTAGTTGTCGTGTGACCTTGGTGTTCTTCCTTTGCCTTTGCTCCAGGTGGGTTGAGACCAATTGATGTATCTT +A00126:224:H7HMWDSX3:3:1114:27760:32002 Test_seq 196937 Test_seq 197403 + - UU A00126:224:H7HMWDSX3:3:1114:27760:32002161Test_seq19693760151M=197256467CACCATTAACTCCTGAAAGCACTCACACACATCCTACCCAACACAACTAAAATAATTCCACCATCTGAGACTCTTATGGAAGAAATGTTACCAAATACAAAGTGCAAGGTAAAATAGCATGGTCTTCTGGACATTTTTCCCTCCCTTGTGCFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:FF:FFFFFFFFF:FFFFFFFFFFF,FFFFFFF:,FFFFFFFFF,FFNM:i:0MD:Z:151MC:Z:148M3SAS:i:151XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1114:27760:3200281Test_seq19725660148M3S=196937-467CTCAACTTCCAGATGGTAAGAAATTTTGTGAGAGTGATGAAATTAAGATACTTGATGCGTCACAGGCCAGGGCAGCAGCACTGCTCAATTCTAGGGGGAATTGCTTCCATGACAGTGTATTGAACCTGTGCTCCAAGGGGTGCCTTTATAGFFFFFFFFFFFFFFF:F:FFF,FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFF:FFFFF:FFFFFFFFFFFFF::FFFFFFFFNM:i:0MD:Z:148MC:Z:151MAS:i:148XS:i:0Yt:Z:UU 60 60 CACCATTAACTCCTGAAAGCACTCACACACATCCTACCCAACACAACTAAAATAATTCCACCATCTGAGACTCTTATGGAAGAAATGTTACCAAATACAAAGTGCAAGGTAAAATAGCATGGTCTTCTGGACATTTTTCCCTCCCTTGTGC CTCAACTTCCAGATGGTAAGAAATTTTGTGAGAGTGATGAAATTAAGATACTTGATGCGTCACAGGCCAGGGCAGCAGCACTGCTCAATTCTAGGGGGAATTGCTTCCATGACAGTGTATTGAACCTGTGCTCCAAGGGGTGCCTTTATAG +A00126:224:H7HMWDSX3:3:1114:28763:32550 Test_seq 169079 Test_seq 169464 + - UU A00126:224:H7HMWDSX3:3:1114:28763:3255097Test_seq16907946151M=169314386CGGTTTCAATCTCTTTTTTTGTTCTGGGTCTATTTAGTTGTTCTACTTCTGAATGTGTTAGTTTAGGTAGGTAGTGTTTTTCTAGGAATTCATCCATTTCTTCTAGGTTTGCAAATTTGTTAGAGTACAATTTTTCATAAGAATCTGAAATFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFNM:i:10MD:Z:1C21A58G1A25T11C11A1G2G0T10MC:Z:151MAS:i:107XS:i:80Yt:Z:UU A00126:224:H7HMWDSX3:3:1114:28763:32550145Test_seq16931460151M=169079-386TTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:NM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:112Yt:Z:UU 46 60 CGGTTTCAATCTCTTTTTTTGTTCTGGGTCTATTTAGTTGTTCTACTTCTGAATGTGTTAGTTTAGGTAGGTAGTGTTTTTCTAGGAATTCATCCATTTCTTCTAGGTTTGCAAATTTGTTAGAGTACAATTTTTCATAAGAATCTGAAAT TTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGAT +A00126:224:H7HMWDSX3:3:1114:30436:32628 Test_seq 167332 Test_seq 400859 + - UU A00126:224:H7HMWDSX3:3:1114:30436:3262897Test_seq1673329109M=400753233528GAGATGTCATAGCTGAATATTCTTTCCCAGTCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:36A72MC:Z:107M2SAS:i:104XS:i:99XA:Z:Test_seq,-213594,109M,2;Test_seq,+400753,107M2S,3;Yt:Z:UU A00126:224:H7HMWDSX3:3:1114:30436:32628145Test_seq4007533107M2S=167332-233528GAGATGTCATAGCTGCAAATTTTTTCCCAATCTGTAGGTGGTCTTTTTACTCTTTTGGAGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATFFFFF:FFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:4MD:Z:15A2G10G28T48MC:Z:109MAS:i:87XS:i:84XA:Z:Test_seq,+213594,109M,5;Test_seq,-167332,109M,6;Yt:Z:UU 9 3 GAGATGTCATAGCTGAATATTCTTTCCCAGTCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTAT GAGATGTCATAGCTGCAAATTTTTTCCCAATCTGTAGGTGGTCTTTTTACTCTTTTGGAGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTAT +A00126:224:H7HMWDSX3:3:1114:28393:32878 Test_seq 168326 Test_seq 212764 - - UU A00126:224:H7HMWDSX3:3:1114:28393:32878177Test_seq1682741353M=21271244439GAGACTCTTCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTTCTTFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:49C3MC:Z:53MAS:i:49XS:i:44XA:Z:Test_seq,+212716,4S49M,1;Yt:Z:UU A00126:224:H7HMWDSX3:3:1114:28393:32878113Test_seq2127121153M=168274-44439AAGGAATCCTCCAGAAAACTACTGAAACTAATAGAAGAGTTTGGCAGAGTCTCFFF,FFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFF::FFFFFFFFFFFFFFFNM:i:1MD:Z:0T52MC:Z:53MAS:i:52XS:i:48XA:Z:Test_seq,+168274,53M,1;Yt:Z:UU 13 11 GAGACTCTTCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTTCTT AAGGAATCCTCCAGAAAACTACTGAAACTAATAGAAGAGTTTGGCAGAGTCTC +A00126:224:H7HMWDSX3:3:1114:16586:33223 Test_seq 166961 Test_seq 167304 + - UU A00126:224:H7HMWDSX3:3:1114:16586:33223161Test_seq1669616151M=167154344GTAGTTCTATTTCTAACTGTTTAAGATAATGCCAGATAGATTTCCAAAGTGGTTATACCATTTTACATTCCCACCAGCAGTGTATGAGAGTTCCAATCTCTCTGCAGCCTCTCCAACATTTATTATTTTGTGTTTTTTGGATTAATGCCAGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:F:FFFFFFFFFFFFFFFFFFFFFFF:FFNM:i:7MD:Z:29C7G16G12A27G6C0A47MC:Z:151MAS:i:116XS:i:111XA:Z:Test_seq,-213923,151M,8;Yt:Z:UU A00126:224:H7HMWDSX3:3:1114:16586:3322381Test_seq16715444151M=166961-344CATTTCTCTAATGGCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAATCATGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFNM:i:1MD:Z:89T61MC:Z:151MAS:i:146XS:i:125XA:Z:Test_seq,+213730,151M,6;Yt:Z:UU 6 44 GTAGTTCTATTTCTAACTGTTTAAGATAATGCCAGATAGATTTCCAAAGTGGTTATACCATTTTACATTCCCACCAGCAGTGTATGAGAGTTCCAATCTCTCTGCAGCCTCTCCAACATTTATTATTTTGTGTTTTTTGGATTAATGCCAG CATTTCTCTAATGGCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAATCATG +A00126:224:H7HMWDSX3:3:1114:18105:33536 ! 0 Test_seq 167562 - - NR A00126:224:H7HMWDSX3:3:1114:18105:3353697Test_seq1671024426S125M=167412461TTAATGTTTCTGTGGCTGAAGTTGAGTTAATGCCAGTCTAGTTGGTGTGAGATGGAATCTCATCGTAGTTTTAATTTGCATTTCTCTAATGGCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCCTTAGTGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFNM:i:2MD:Z:42C75T6MC:Z:151MAS:i:115XS:i:95XA:Z:Test_seq,-213808,125M26S,6;Yt:Z:NR A00126:224:H7HMWDSX3:3:1114:18105:33536145Test_seq16741260151M=167102-461GTGTTTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATGCCCTGTATTAGGGCTCCTAGGGTAGATCCTATTTTTTCTTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:99T51MC:Z:26S125MAS:i:146XS:i:107Yt:Z:NR 0 60 GTGTTTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATGCCCTGTATTAGGGCTCCTAGGGTAGATCCTATTTTTTCTTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGG +A00126:224:H7HMWDSX3:3:1114:13801:36354 Test_seq 191422 Test_seq 191816 + - UU A00126:224:H7HMWDSX3:3:1114:13801:36354161Test_seq19142260151M=191666395TCTCTTGTGGCTGTGTAGCAAGACTGTTAAAGTGCTCAGTGCCTGACAGAAATTTAAACTCCCAATGAGAGATTTTGCTGCAATGGAGTAGAAAGTTGAAATTTAAGAAAAGAAGACTAAGCTGCCTCTCAGAGAATAGCTCCAGAAGGCTFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1114:13801:3635481Test_seq19166660151M=191422-395CTTAAAACTAGCCCAAGCCCTCAGCAAACCACAGAAACGTAAACTACCACAGAGGGGATAATCTGGCTGGAGCAGGGTGGAGCGGGTGACCTAACCCGTTTCTCCACCGTGCACCACTGCGAGGGCTCCGGTTCTGCATGCCTCTTGCTTGFFFFF,FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU 60 60 TCTCTTGTGGCTGTGTAGCAAGACTGTTAAAGTGCTCAGTGCCTGACAGAAATTTAAACTCCCAATGAGAGATTTTGCTGCAATGGAGTAGAAAGTTGAAATTTAAGAAAAGAAGACTAAGCTGCCTCTCAGAGAATAGCTCCAGAAGGCT CTTAAAACTAGCCCAAGCCCTCAGCAAACCACAGAAACGTAAACTACCACAGAGGGGATAATCTGGCTGGAGCAGGGTGGAGCGGGTGACCTAACCCGTTTCTCCACCGTGCACCACTGCGAGGGCTCCGGTTCTGCATGCCTCTTGCTTG +A00126:224:H7HMWDSX3:3:1115:10854:1626 ! 0 Test_seq 168697 - - NR A00126:224:H7HMWDSX3:3:1115:10854:162697Test_seq1683476029S122M=168547351AAGAAGGGCTACATGTATTTACAGATCGATCATGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCTTGCCAATCCGGATACCCTTTATTTCTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGCGGTFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFNM:i:2MD:Z:18A28T74MC:Z:151MAS:i:112XS:i:66Yt:Z:NR A00126:224:H7HMWDSX3:3:1115:10854:1626145Test_seq16854760151M=168347-351GTTGGCTTTGCATAGGTGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATCATGTGGTTTTTATCTTTTGTTTFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFNM:i:1MD:Z:15A135MC:Z:29S122MAS:i:146XS:i:76Yt:Z:NR 0 60 GTTGGCTTTGCATAGGTGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATCATGTGGTTTTTATCTTTTGTTT +A00126:224:H7HMWDSX3:3:1115:12454:2425 Test_seq 169083 Test_seq 169467 + - UU A00126:224:H7HMWDSX3:3:1115:12454:242597Test_seq16908360151M=169317385TTCAATCTCTTTTTTTGTTATGGGTCTATTTAGTTGTTCTACTTCGGAATGTGTTAGTTTAGGTAGGTAGTGTTTTTCCAGGAATTCATCCATTTCTTCTAGGTTTTCAAATTTGTTGGAGTACAATTTTTCATAATAATCTGAAATGATTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:FFFFFFFFFNM:i:8MD:Z:45T32G1A36A0C11A1G2G15MC:Z:151MAS:i:111XS:i:76Yt:Z:UU A00126:224:H7HMWDSX3:3:1115:12454:2425145Test_seq16931760151M=169083-385AGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGAFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:150G0MC:Z:151MAS:i:150XS:i:112Yt:Z:UU 60 60 TTCAATCTCTTTTTTTGTTATGGGTCTATTTAGTTGTTCTACTTCGGAATGTGTTAGTTTAGGTAGGTAGTGTTTTTCCAGGAATTCATCCATTTCTTCTAGGTTTTCAAATTTGTTGGAGTACAATTTTTCATAATAATCTGAAATGATT AGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGA +A00126:224:H7HMWDSX3:3:1115:19262:2409 Test_seq 166958 Test_seq 167295 + - UU A00126:224:H7HMWDSX3:3:1115:19262:2409161Test_seq1669587151M=167145338ATGGTAGTTCTATTTCTAACTGTTTAAGATAACGCCAGATAGATTTCCAAAGTGGTTGTACCATTTTACATTCCCACCAGCAGTGTATGAGAGTTCCAATCTCTCCGCAGCCTCTCCAACATTTATTATTTTGTGTTTTTTGGATTAATGCFF:FFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF,FFFFFFFFFFFFFFF:FFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:,:FFFF:,:FFF::FF,FFFFFF:FFFF:FNM:i:4MD:Z:40G29A27G7A44MC:Z:151MAS:i:131XS:i:126XA:Z:Test_seq,-213926,151M,5;Yt:Z:UU A00126:224:H7HMWDSX3:3:1115:19262:240981Test_seq16714542151M=166958-338TTTAATTTGCATTTCTCTAATGGCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:F:FFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFF:FFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:98T52MC:Z:151MAS:i:146XS:i:126XA:Z:Test_seq,+213739,151M,5;Yt:Z:UU 7 42 ATGGTAGTTCTATTTCTAACTGTTTAAGATAACGCCAGATAGATTTCCAAAGTGGTTGTACCATTTTACATTCCCACCAGCAGTGTATGAGAGTTCCAATCTCTCCGCAGCCTCTCCAACATTTATTATTTTGTGTTTTTTGGATTAATGC TTTAATTTGCATTTCTCTAATGGCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGAC +A00126:224:H7HMWDSX3:3:1115:21224:2581 Test_seq 169351 Test_seq 169734 + - UU A00126:224:H7HMWDSX3:3:1115:21224:258197Test_seq16935160151M=169584384ATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGATTCTTTTGTTGCTCACTTTCTATTTGTTCAAGTTFFFFFFFFFF:FFFFFFFF,:,FFF::FFFFF:F:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF:F,FFFFFF:FF,FFFFF:F:,F:FFFFFFFFFFFFFFFF,FF,FF:,F,FFFFFFFF::FFF:FFFFFFFFFFFF,F,FFFNM:i:1MD:Z:116G34MC:Z:151MAS:i:146XS:i:101Yt:Z:UU A00126:224:H7HMWDSX3:3:1115:21224:2581145Test_seq16958460151M=169351-384TTTTGCTGTGTCCCAGAGGTTTTGATAGGAAGTATTTTCATTCTCGTTGCTTTCTATGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTATTGTTCATTTTCCAAGTATTTGATTTCTTTTCCCTCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFNM:i:4MD:Z:44T11A22G70A0MC:Z:151MAS:i:135XS:i:22Yt:Z:UU 60 60 ATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGATTCTTTTGTTGCTCACTTTCTATTTGTTCAAGTT TTTTGCTGTGTCCCAGAGGTTTTGATAGGAAGTATTTTCATTCTCGTTGCTTTCTATGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTATTGTTCATTTTCCAAGTATTTGATTTCTTTTCCCTC +A00126:224:H7HMWDSX3:3:1115:19804:3380 Test_seq 169004 Test_seq 169468 + - UU A00126:224:H7HMWDSX3:3:1115:19804:3380161Test_seq16900460151M=169318465CTCTGAAAGTTTGGTAGAACTCTGCAGTGAAGCCGTCTGGGCCAGGGCTTTTTTTTGTTGGGAGTTTTTTGATTACCGTTTCAATCTCTTTTTTTGTTATGGGTCTACTTAGTTGTTCTACTTCTGAATGTGTTAGGTTAGGTAGGTAGTGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFF:FFFFFFFFFFF:FFFFFF:F:FFFFF:F,FFFFFF:,FF,FFFNM:i:5MD:Z:7G38A14A45T28T14MC:Z:151MAS:i:126XS:i:78Yt:Z:UU A00126:224:H7HMWDSX3:3:1115:19804:338081Test_seq16931860151M=169004-465GTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGAT:FFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:149G1MC:Z:151MAS:i:149XS:i:112Yt:Z:UU 60 60 CTCTGAAAGTTTGGTAGAACTCTGCAGTGAAGCCGTCTGGGCCAGGGCTTTTTTTTGTTGGGAGTTTTTTGATTACCGTTTCAATCTCTTTTTTTGTTATGGGTCTACTTAGTTGTTCTACTTCTGAATGTGTTAGGTTAGGTAGGTAGTG GTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGAT +A00126:224:H7HMWDSX3:3:1115:20320:5431 Test_seq 169004 Test_seq 169468 + - UU A00126:224:H7HMWDSX3:3:1115:20320:5431161Test_seq16900460151M=169318465CTCTGAAAGTTTGGTAGAACTCTGCAGTGAAGCCGTCTGGGCCAGGGCTTTTTTTTGTTGGGAGTTTTTTGATTACCGTTTCAATCTCTTTTTTTGTTATGGGTCTACTTAGTTGTTCTACTTCTGAATGTGTTAGTTTAGGTAGGTAGTGFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF,F:FFFFFFFFFFFFF,F:FFFFF:FFF:FFFFFFFFFFFFFF,:F:FFF::FF:FF,FFFF:FFFFFNM:i:4MD:Z:7G38A14A45T43MC:Z:151MAS:i:131XS:i:83Yt:Z:UU A00126:224:H7HMWDSX3:3:1115:20320:543181Test_seq16931860151M=169004-465GTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGATFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FF,FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:149G1MC:Z:151MAS:i:149XS:i:112Yt:Z:UU 60 60 CTCTGAAAGTTTGGTAGAACTCTGCAGTGAAGCCGTCTGGGCCAGGGCTTTTTTTTGTTGGGAGTTTTTTGATTACCGTTTCAATCTCTTTTTTTGTTATGGGTCTACTTAGTTGTTCTACTTCTGAATGTGTTAGTTTAGGTAGGTAGTG GTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGAT +A00126:224:H7HMWDSX3:3:1115:8187:6903 Test_seq 290254 Test_seq 290583 + - UU A00126:224:H7HMWDSX3:3:1115:8187:6903161Test_seq29025460151M=290434330ATGTGAAGAATGTAACCAATGGCATTGATCTGTATGTGTAGAAATTGTTGAATGGGTGTATGTTATGTATATTTTCAACCAAAATTAAAAAAAAAAAAAAAGAGAAAGAAGAGGATGGGACCAGAAGGGCAAGGCCACAGGGGCATGACCAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FF,FF:FFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:32M1I118MAS:i:151XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1115:8187:690381Test_seq2904346032M1I118M=290254-330GAGCAGGGGCACGTTTGCTCAAGTATAAGGGTGGTAGTGTAGTTTACCATCACCAAGAATTATTAGGATATTAACTCACTTTAGACTTAAGATAGCAGTATGTCACTTAAAGAAATTCCAGACCTAGGCAAGATTTTTGCCTTCAGACCTCFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:150MC:Z:151MAS:i:143XS:i:0Yt:Z:UU 60 60 ATGTGAAGAATGTAACCAATGGCATTGATCTGTATGTGTAGAAATTGTTGAATGGGTGTATGTTATGTATATTTTCAACCAAAATTAAAAAAAAAAAAAAAGAGAAAGAAGAGGATGGGACCAGAAGGGCAAGGCCACAGGGGCATGACCA GAGCAGGGGCACGTTTGCTCAAGTATAAGGGTGGTAGTGTAGTTTACCATCACCAAGAATTATTAGGATATTAACTCACTTTAGACTTAAGATAGCAGTATGTCACTTAAAGAAATTCCAGACCTAGGCAAGATTTTTGCCTTCAGACCTC +A00126:224:H7HMWDSX3:3:1115:12274:7623 Test_seq 213781 Test_seq 214225 + - UU A00126:224:H7HMWDSX3:3:1115:12274:762397Test_seq21378160151M=214075445GTGGGCAAAGGATATGAACACGCTCTTCACTAAAGAAGATAGTCAGGCGGCTAACAGATACATGAGAAAATGCTCTCGATTATTAGCCATTAGAGAAATGCAAATTAAAACTACGATGAGATTCCATCTCACTCCAACAAGGCTGGCATTAF:F::F:F,:FFF:,:F:FFF,::FFFFF:FFF::FFFFFFFFFF:F,FFFFFFFFFF:FFFF:FFFFFFF,FFFFFFFFFFFFFF,FFFFF,FFF,:FFF,FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF::FFF,:FFFFFFF:NM:i:5MD:Z:23A17T6A31C29G40MC:Z:151MAS:i:126XS:i:91Yt:Z:UU A00126:224:H7HMWDSX3:3:1115:12274:7623145Test_seq21407560151M=213781-445ATACAACCCAGAAATCCCACTCCTCGGAATATACCCTAGAGAAATAAGAGCCTTCACACAAACAGATATATGCACACCCATGTTTATTGCAGCTCTGTTTACAATAGCAAAAAGCTGGAAGCAACCAAGGTGTCCATCAAAGGATGAATGGFFF,FFF:FFFFFFF,F:FFFFF::,FFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFF,FFF:FF,FF::FFFFF::,FFF:FFFF:FF,F:FFF:FFFFFFF:F,FFFFFFF,F::FFF,FFFFFFFFFFFF:FFF:FFFFFF:F:FFFNM:i:1MD:Z:140T10MC:Z:151MAS:i:146XS:i:103Yt:Z:UU 60 60 GTGGGCAAAGGATATGAACACGCTCTTCACTAAAGAAGATAGTCAGGCGGCTAACAGATACATGAGAAAATGCTCTCGATTATTAGCCATTAGAGAAATGCAAATTAAAACTACGATGAGATTCCATCTCACTCCAACAAGGCTGGCATTA ATACAACCCAGAAATCCCACTCCTCGGAATATACCCTAGAGAAATAAGAGCCTTCACACAAACAGATATATGCACACCCATGTTTATTGCAGCTCTGTTTACAATAGCAAAAAGCTGGAAGCAACCAAGGTGTCCATCAAAGGATGAATGG +A00126:224:H7HMWDSX3:3:1115:13892:9987 ! 0 Test_seq 169352 - + NR A00126:224:H7HMWDSX3:3:1115:13892:998781Test_seq16961760130M21S=169352-395ATTTTCATTCTCGTTGCTTTCTATGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTATTGTTCATTTTCCAAGTATTTGATTTCTTTTCCCTCGTTCTTCTGTTATTGATCGATCAGAAAGGTGCCFFFFFFF,FFFFF,FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:5MD:Z:11T11A22G70A3T8MC:Z:151MAS:i:105XS:i:0Yt:Z:NR A00126:224:H7HMWDSX3:3:1115:13892:9987161Test_seq16935260151M=169617395TTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGATTCTTTTGTTGCTCACTTTCTATTTGTTCAAGTTGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFF:FFFFF,FFF:FFFFFFFFFFFFFFF:FFFF:FF,FFF:,FFF:F:FFFFFF:FFFFFFFF,,FFFFFFFFF:F:F:,FF:FFFFFFFFF,F,FF,NM:i:1MD:Z:115G35MC:Z:130M21SAS:i:146XS:i:101Yt:Z:NR 0 60 TTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGATTCTTTTGTTGCTCACTTTCTATTTGTTCAAGTTG +A00126:224:H7HMWDSX3:3:1115:16803:13808 Test_seq 168997 Test_seq 169469 + - UU A00126:224:H7HMWDSX3:3:1115:16803:13808161Test_seq16899760151M=169319473AACTCTTCTCTGAAAATTTGGTAGAACTCTGCAGTGAAGCCGTCCGGGCCAGGACTTTTTTTTGTTGGGAGTTTTTTGATTACCGTTTCAATCTCTTTTTTTGTTATGGGTCTATTTAGTTGTTCTGCTTCTGAATGTGTTAGTTTAGGTAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFF::F:FFFF:F:FF,FFF,F,:FFF:FFFFFFFFFFFFFF:FF::FFFFFF::::FFFF,,FFFFFF,FFFFFFFFFFFFFF,F:FFFFF,,:FF:F,NM:i:6MD:Z:2G11G0G28T23A57A24MC:Z:151MAS:i:123XS:i:65Yt:Z:UU A00126:224:H7HMWDSX3:3:1115:16803:1380881Test_seq16931960151M=168997-473TCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGATTFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFNM:i:1MD:Z:148G2MC:Z:151MAS:i:148XS:i:112Yt:Z:UU 60 60 AACTCTTCTCTGAAAATTTGGTAGAACTCTGCAGTGAAGCCGTCCGGGCCAGGACTTTTTTTTGTTGGGAGTTTTTTGATTACCGTTTCAATCTCTTTTTTTGTTATGGGTCTATTTAGTTGTTCTGCTTCTGAATGTGTTAGTTTAGGTA TCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGATT +A00126:224:H7HMWDSX3:3:1115:4915:16673 ! 0 Test_seq 401000 - - MU A00126:224:H7HMWDSX3:3:1115:4915:1667397Test_seq151752055M=400946249249TTATGCCATGTGTTAGGGCTCCTAGCATTGTCCCTATTTTTTCTTCCATGATCTAFFFFF:F,:F,:FFF:FFFFFFFF,FFFFF,,:F,FFFF,FFF:F,FFFF,,F:FNM:i:4MD:Z:11A11C0A29T0MC:Z:55MAS:i:39XS:i:39XA:Z:Test_seq,-213497,55M,4;Test_seq,+49445,53M2S,3;Test_seq,+19104,36M1D19M,4;Test_seq,-41170,55M,5;Test_seq,+400946,55M,5;Yt:Z:MU A00126:224:H7HMWDSX3:3:1115:4915:16673145Test_seq4009466055M=151752-249249TTATGCCTTGTATTAGGGCTCCTAACGTTGTCCCTATTTTTTCTTCCATGATGTTFFFF::FFFFF:FFF,FF:FFFF,,:FF::F:F:F:FFFF:FFFF:FFFFFF,FFNM:i:1MD:Z:52C2MC:Z:55MAS:i:52XS:i:0Yt:Z:MU 0 60 TTATGCCATGTGTTAGGGCTCCTAGCATTGTCCCTATTTTTTCTTCCATGATCTA TTATGCCTTGTATTAGGGCTCCTAACGTTGTCCCTATTTTTTCTTCCATGATGTT +A00126:224:H7HMWDSX3:3:1115:12599:18677 Test_seq 219279 Test_seq 219579 + - UU A00126:224:H7HMWDSX3:3:1115:12599:1867797Test_seq21927960151M=219429301GAAATTCAGGAAGCAGAGCTTCCTCAAGTCTTAAAGGGTAGGCTCACAAACTCTGGAGTCTCAAAGAGCGGAACCATAGCCTGGAGCCTCAAAGAGTGGAACCACAGCCTCAAAGGGTGAGACCACAGCGTCCTAGGTATCAAAGAGTCAGFFFF::FFFFFFFFF:F:FF:FFFF:FFFFFFFFFFFF:F:,FFFFFFFFF::FFFFF:FFF:F,F:FF::FFFFFFFFFF:FFFFFFFFFF,FFFFFFFFFFFFFFF:FFFFFFF,,FFFFFFFF,::FFF::,FFFFFFFFF,FF,FF:NM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1115:12599:18677145Test_seq21942960151M=219279-301GATCTTCAGTAAATCTGTCCTGTTGTGTGGGGCTGCCTTTCACGTGTGCTGGGGAAATGGCCCCAGATCCACTGTCCAAAGCCGTGGGGGTGGGACTGCCATGGCCAAGGGACAGAAGGACAGAGACTGCCGGAGCAGAGGGGGTGGGATC:FFF,FFF,,F,,FFF,,:,FF,,,:FFFFFF,FFFFF:FF,F,,FF,:,FF:,,FF:F,,F,F,:F:FF:F,F:FFFF:F:FF,FFFF:FFF,FFFFF,FFFFFF,,F:FF:F,F,FFFF,:F,FFF::,F:FFFF,,:F,FF,:FFFFFNM:i:5MD:Z:8C13G0A20A39A66MC:Z:151MAS:i:126XS:i:0Yt:Z:UU 60 60 GAAATTCAGGAAGCAGAGCTTCCTCAAGTCTTAAAGGGTAGGCTCACAAACTCTGGAGTCTCAAAGAGCGGAACCATAGCCTGGAGCCTCAAAGAGTGGAACCACAGCCTCAAAGGGTGAGACCACAGCGTCCTAGGTATCAAAGAGTCAG GATCTTCAGTAAATCTGTCCTGTTGTGTGGGGCTGCCTTTCACGTGTGCTGGGGAAATGGCCCCAGATCCACTGTCCAAAGCCGTGGGGGTGGGACTGCCATGGCCAAGGGACAGAAGGACAGAGACTGCCGGAGCAGAGGGGGTGGGATC +A00126:224:H7HMWDSX3:3:1115:4652:19038 Test_seq 214124 Test_seq 261704 + + UU A00126:224:H7HMWDSX3:3:1115:4652:19038129Test_seq2141243260M=26170447581GCCTTCACACAAACAGATATATGCACACCCATGTTTATTGCAGCTCTGTTTACAATAGCAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:60MC:Z:60MAS:i:60XS:i:50XA:Z:Test_seq,-166851,60M,2;Yt:Z:UU A00126:224:H7HMWDSX3:3:1115:4652:1903865Test_seq2617042660M=214124-47581TGCTCTTGTAAACAGAGCTGCAATAAACATGGGCGTGCATGTATCTGTTCGTGTAAAGGCFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:FFFFFFFFNM:i:3MD:Z:4A21G6T26MC:Z:60MAS:i:45XS:i:35Yt:Z:UU 32 26 GCCTTCACACAAACAGATATATGCACACCCATGTTTATTGCAGCTCTGTTTACAATAGCA TGCTCTTGTAAACAGAGCTGCAATAAACATGGGCGTGCATGTATCTGTTCGTGTAAAGGC +A00126:224:H7HMWDSX3:3:1115:14696:21057 Test_seq 168933 Test_seq 169233 + - UU A00126:224:H7HMWDSX3:3:1115:14696:21057161Test_seq16893360151M=169097301GAATGAGTTGGGTAGTATTCCGTCTTTTTCTATGCTTTGAAATACCTTCAGTAGTAGTGGTGTTAAGTCTTCTCTGAAGGTTTGGTAGAACTCTGCAGTGAAGCCGTCTGGGCCAGGACTTTTTTTTGTTGGAAGTTTTTTGATTACCGTTFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFF:FFF:FF:FFFFFFFFFFF:F,FFFF:FFFFFFFFFFFF,:::FFFFFFFF,FFF:FFFFFFFF:FFFFNM:i:1MD:Z:26G124MC:Z:137M14SAS:i:146XS:i:75Yt:Z:UU A00126:224:H7HMWDSX3:3:1115:14696:2105781Test_seq16909760137M14S=168933-301TTGTTATGGGTCTATTTAGTTGTTCTACTTCTGAATGTGTTCGTTTAGGTAGGTAGTATTTTTCCAAGAATTTATCCATTTCTTCTAGGTTTTCAAATTTGTTAGAGTACAATTTTACATAGTAATCTGAAATGATTTAAGCAGATGTCTT:FFFF,FFFFFF,FFFFFFFFFFFFF::,FFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFF,FFF:FFFFFFFFFFFF:FF:FFFFFF:FFFFFFF,FFF:FNM:i:6MD:Z:41A15G6G7C31C13G18MC:Z:151MAS:i:107XS:i:71Yt:Z:UU 60 60 GAATGAGTTGGGTAGTATTCCGTCTTTTTCTATGCTTTGAAATACCTTCAGTAGTAGTGGTGTTAAGTCTTCTCTGAAGGTTTGGTAGAACTCTGCAGTGAAGCCGTCTGGGCCAGGACTTTTTTTTGTTGGAAGTTTTTTGATTACCGTT TTGTTATGGGTCTATTTAGTTGTTCTACTTCTGAATGTGTTCGTTTAGGTAGGTAGTATTTTTCCAAGAATTTATCCATTTCTTCTAGGTTTTCAAATTTGTTAGAGTACAATTTTACATAGTAATCTGAAATGATTTAAGCAGATGTCTT +A00126:224:H7HMWDSX3:3:1115:17580:21605 Test_seq 167749 Test_seq 168156 + - UU A00126:224:H7HMWDSX3:3:1115:17580:21605161Test_seq1677491151M=168019408TTCTCAATTCTGTTCCATTGGTCTATGTGCCTGTTGTTGTACCAGTACCAGGCTGTTTTGACTACTGTAGCTGTATAATAGGTTCTGAAATCAGGTAGAGTGAGGCCTCCCACTTTCTTCTTCTTTTTCAGTAATGCTTTGCTTATCCGGG:FFFFF,,:FFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF,FFFFFFF:FFFFFFF:FFFFFFFFFF,FFFFFFFFFFFFFFFF,FF:F:,FFF,FFF:FFFF:FFFF:FFFFF:FF:F:FNM:i:1MD:Z:69C81MC:Z:138M13SAS:i:146XS:i:144XA:Z:Test_seq,-213139,151M,2;Yt:Z:UU A00126:224:H7HMWDSX3:3:1115:17580:2160581Test_seq16801960138M13S=167749-408TTTACTATGTTAAGTCTTCCTATCCATGAGCAGGGTATGTTTTTCCACTTAAGTATGTCCTTTTGAATTTCTTGTAGCAGAGTTTTATAGTTTTCTTTGTATAGGTCTTTTACATTCTTGGTAAGATTTATTCCTAAGAGCATTGGCATTGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFFF,FFFF,FFFFF,FFFF::FFFFFFFFFFFFF:FFFFF,FFFFFFFFF:FFF:FFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFF::FFFFFFFFFFFFNM:i:5MD:Z:41C58G12C1C3C18MC:Z:151MAS:i:113XS:i:78Yt:Z:UU 1 60 TTCTCAATTCTGTTCCATTGGTCTATGTGCCTGTTGTTGTACCAGTACCAGGCTGTTTTGACTACTGTAGCTGTATAATAGGTTCTGAAATCAGGTAGAGTGAGGCCTCCCACTTTCTTCTTCTTTTTCAGTAATGCTTTGCTTATCCGGG TTTACTATGTTAAGTCTTCCTATCCATGAGCAGGGTATGTTTTTCCACTTAAGTATGTCCTTTTGAATTTCTTGTAGCAGAGTTTTATAGTTTTCTTTGTATAGGTCTTTTACATTCTTGGTAAGATTTATTCCTAAGAGCATTGGCATTG +A00126:224:H7HMWDSX3:3:1115:28266:23422 Test_seq 167524 Test_seq 167949 + - UU A00126:224:H7HMWDSX3:3:1115:28266:23422161Test_seq16752452151M=167814426TCTTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGGTATGCCAGCACCATTTGTTAAAAAGACTATFFFFFFFFFFFFFFFFFFF::FFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:,FFFFFFFFFFFFFF,FNM:i:1MD:Z:120T30MC:Z:136M15SAS:i:146XS:i:121XA:Z:Test_seq,-213364,96M2I23M2I28M,6;Yt:Z:UU A00126:224:H7HMWDSX3:3:1115:28266:2342281Test_seq16781460136M15S=167524-426TGTAGCTATATAATAGGTTCTGAAATCAGGTAGGGTGAGGCCTCCCACTTTCTTCTTCTTTTTCAGTAATGTTTTGCTTATCCGGGGGTTCTTTCCCTTCCATATGAAATTAGTGATTTGTTTCTCTATCCCCTTACTCATAACCATTCATFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FF,FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:4MD:Z:4C2G25A37C64MC:Z:151MAS:i:118XS:i:81Yt:Z:UU 52 60 TCTTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGGTATGCCAGCACCATTTGTTAAAAAGACTAT TGTAGCTATATAATAGGTTCTGAAATCAGGTAGGGTGAGGCCTCCCACTTTCTTCTTCTTTTTCAGTAATGTTTTGCTTATCCGGGGGTTCTTTCCCTTCCATATGAAATTAGTGATTTGTTTCTCTATCCCCTTACTCATAACCATTCAT +A00126:224:H7HMWDSX3:3:1115:28583:23719 ! 0 Test_seq 166075 - + NR A00126:224:H7HMWDSX3:3:1115:28583:2371981Test_seq16642960125M26S=166075-479CTTTGAAGCTTTCATTTTATCCCGGAAACCTTTTTGCTTTTAGTCCAGTCCAATTAGGCTGACCTTCCTTGTATTGAGTGTTGTCTTTCCCTTCACCCAAAGCAGTTCTTATCTACTGACTGATCGATCAGGGGAGAGCTAATACACTTATFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:3MD:Z:31C44T42T5MC:Z:151MAS:i:110XS:i:40Yt:Z:NR A00126:224:H7HMWDSX3:3:1115:28583:23719161Test_seq16607560151M=166429479CCTGCTCCCTCAGGAGTTGTCTGTTGTGCTCCCTGACAGGGCAGACATCGATTGTGGCCGGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCTTAGTTGTCGTGTGACFFFFFFFFF,FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFF:F:FF:FFFFFFFFNM:i:1MD:Z:149G1MC:Z:125M26SAS:i:149XS:i:46Yt:Z:NR 0 60 CCTGCTCCCTCAGGAGTTGTCTGTTGTGCTCCCTGACAGGGCAGACATCGATTGTGGCCGGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCTTAGTTGTCGTGTGAC +A00126:224:H7HMWDSX3:3:1115:22878:24486 Test_seq 167180 Test_seq 167664 + - UU A00126:224:H7HMWDSX3:3:1115:22878:24486161Test_seq16718060151M=167514485AGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAATCATGTAGATTTTAGAGATCAGGAGCTGGTCFF:FFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFF,FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFF:F:FFFFFFFFFF:FFFF:FFFFFFFFF,FF:F::,FFFFFF:FFFFF:FFFF,FFNM:i:1MD:Z:63T87MC:Z:151MAS:i:146XS:i:116Yt:Z:UU A00126:224:H7HMWDSX3:3:1115:22878:2448681Test_seq16751449151M=167180-485CCCTATTTTTTCTTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGGTATGCCGGCACCATTTGTTAFFFFFF:FFFFFFFFF:FFFFFFF,FFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:3MD:Z:0T129T6A13MC:Z:151MAS:i:140XS:i:116XA:Z:Test_seq,+213374,86M2I23M2I38M,7;Yt:Z:UU 60 49 AGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAATCATGTAGATTTTAGAGATCAGGAGCTGGTC CCCTATTTTTTCTTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGGTATGCCGGCACCATTTGTTA +A00126:224:H7HMWDSX3:3:1115:11966:24846 Test_seq 162525 Test_seq 362348 + - UR A00126:224:H7HMWDSX3:3:1115:11966:2484697Test_seq1625252423M128S=362198199824CTCCTGGGTAGTTAGATGGGATCACTCAGCAGGAATTATGCGGTGCATTTTTCTGTAGGTTAAGTCGTCTAGGGAGCTGAAGGATAGTCTCATTAGTTCTCAACCTCTTCTCCATACCTTTTGTGGGAGTGCACACACATGCGCCCACGTGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:23MC:Z:151MAS:i:23XS:i:0SA:Z:Test_seq,361994,+,23S128M,60,0;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1115:11966:248462145Test_seq3619946023H128M=362198355ACTCAGCAGGAATTATGCGGTGCATTTTTCTGTAGGTTAAGTCGTCTAGGGAGCTGAAGGATAGTCTCATTAGTTCTCAACCTCTTCTCCATACCTTTTGTGGGAGTGCACACACATGCGCCCACGTGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:128MC:Z:151MAS:i:128XS:i:0SA:Z:Test_seq,162525,+,23M128S,24,0;Yt:Z:UR A00126:224:H7HMWDSX3:3:1115:11966:24846145Test_seq36219860151M=162525-199824TTGTCTTTTTCTGTTTAAAACTATTTGTGCTTATCGTGGACAATATTGAAAGTAATAAGAAAGGGTAAAACATCCTTTGAAAGTCTGGTAAATGAGAACACACTACTGCATTTTCCTTTCCTTTTTCTTGTAAGCATAGTTTTTGTCTGTTFFF:F,FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFNM:i:0MD:Z:151MC:Z:23M128SAS:i:151XS:i:0Yt:Z:UR 24 60 CTCCTGGGTAGTTAGATGGGATCACTCAGCAGGAATTATGCGGTGCATTTTTCTGTAGGTTAAGTCGTCTAGGGAGCTGAAGGATAGTCTCATTAGTTCTCAACCTCTTCTCCATACCTTTTGTGGGAGTGCACACACATGCGCCCACGTG TTGTCTTTTTCTGTTTAAAACTATTTGTGCTTATCGTGGACAATATTGAAAGTAATAAGAAAGGGTAAAACATCCTTTGAAAGTCTGGTAAATGAGAACACACTACTGCATTTTCCTTTCCTTTTTCTTGTAAGCATAGTTTTTGTCTGTT +A00126:224:H7HMWDSX3:3:1115:1967:25974 Test_seq 167150 Test_seq 167599 + - UU A00126:224:H7HMWDSX3:3:1115:1967:2597497Test_seq16715018151M=167449450TTTGCATTTCTCTAATGGCTAATGATCGGGAGCATTTTCTCATGTATCTGTTAGCTGCCTGAATATCTTCTTTAGCGAAATGTGTGTTCATGTCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTCACAGAATFFFFFFFFFFFFFF:F,,FFFFF:FFFFFFFFFFFFFFFF:FFFFFFFF,FFFFFFFF:FFF,FFF:FF,FFFFFFFFFFF,FFFFFFFFF,F,,FFFF:::FFF:FFFF,FFFF,F:FFFF:F,,FFFF,FFFFFFFFF:FF:F:FFF:FNM:i:6MD:Z:28A23G22T15A1T49G7MC:Z:151MAS:i:121XS:i:111XA:Z:Test_seq,-213734,151M,8;Yt:Z:UU A00126:224:H7HMWDSX3:3:1115:1967:25974145Test_seq16744960151M=167150-450TCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATGCCCTGTATTAGGGCTCCTAGGGTTGATCCTATTTTTTCTTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTG:FFF:FFF,:FFFFF:FFFFF:FFFFF,FFFFF:FFFFFF:FFFF:FFFF:FFF,FFFFFFFFFFF,FFFFFFFFFFFF:FFFFFFFFFFFF,F,FFFFF:::FFFFFFFF:F:F,FFFFFFF:FF:FFFFFFFFFFFF:FFFFFFFFFF,NM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:101Yt:Z:UU 18 60 TTTGCATTTCTCTAATGGCTAATGATCGGGAGCATTTTCTCATGTATCTGTTAGCTGCCTGAATATCTTCTTTAGCGAAATGTGTGTTCATGTCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTCACAGAAT TCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATGCCCTGTATTAGGGCTCCTAGGGTTGATCCTATTTTTTCTTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTG +A00126:224:H7HMWDSX3:3:1115:7789:25974 ! 0 Test_seq 167947 - - NR A00126:224:H7HMWDSX3:3:1115:7789:25974161Test_seq1675683432S119M=167797380CATATTTATGCCTATTACCAAGAAAGGTGATCGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGCTATGCCAGCACCATTTGTTAAAAAGACTATCATTTCCCCAATFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FNM:i:1MD:Z:76T42MC:Z:151MAS:i:114XS:i:99XA:Z:Test_seq,-213352,108M2I9M32S,4;Yt:Z:NR A00126:224:H7HMWDSX3:3:1115:7789:2597481Test_seq16779760151M=167568-380CAGGCTGTTTTGACTACTGTAGCTGTATAATAGGTTCTGAAATCAGGTAGAGTGAGGCCTCCCACTTTCTTCTTCTTTTTCAGTAATGCTTTGCTTATCCGGGGGTTCTTTCCCTTCCATATGAAATTAGTGATTTGTTTCTCTATCCCCTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:21C129MC:Z:32S119MAS:i:146XS:i:113Yt:Z:NR 0 60 CAGGCTGTTTTGACTACTGTAGCTGTATAATAGGTTCTGAAATCAGGTAGAGTGAGGCCTCCCACTTTCTTCTTCTTTTTCAGTAATGCTTTGCTTATCCGGGGGTTCTTTCCCTTCCATATGAAATTAGTGATTTGTTTCTCTATCCCCT +A00126:224:H7HMWDSX3:3:1115:18032:26678 Test_seq 170112 Test_seq 170470 + - UU A00126:224:H7HMWDSX3:3:1115:18032:26678161Test_seq17011260151M=170320359TGCATAAATATTTAATATGGTTATGTCTTCCTGATCAATTGTCCCTTTTATCATTATATAGTGTCCTTCTTTATCCTTTGTGGCGGATTTAAGTCTAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCCTCTTCTTTTTTGCTTATTAFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFF,FFFFFFFFFFFFFFFFFFFFF:F,:FFFFFFFFFFFFFFFFFFF:,FFFFFFFFFFFF:FFFFFFFFF:FF:FF:FFF::FFFFFFFFFFF,FFF:FFNM:i:3MD:Z:3G20A59A66MC:Z:151MAS:i:137XS:i:53Yt:Z:UU A00126:224:H7HMWDSX3:3:1115:18032:2667881Test_seq17032060151M=170112-359CTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCTGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTTFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:56C94MC:Z:151MAS:i:146XS:i:48Yt:Z:UU 60 60 TGCATAAATATTTAATATGGTTATGTCTTCCTGATCAATTGTCCCTTTTATCATTATATAGTGTCCTTCTTTATCCTTTGTGGCGGATTTAAGTCTAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCCTCTTCTTTTTTGCTTATTA CTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCTGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTT +A00126:224:H7HMWDSX3:3:1115:6686:27383 Test_seq 166310 Test_seq 166674 + - RU A00126:224:H7HMWDSX3:3:1115:6686:2738397Test_seq16631060151M=166550365CCCAGGCGCCACAATTCAAAGTGGGATGCAGAATGTTTTCATAATAGAATTATTTTGCCCGTTGACTTAGAAGTCTCCTCAAACCATGTTCCCCAGACCCCAGCCCCTGCTCCGCTGACCTTTGAAGCTTTCATTTTATCCCGGAAACCTCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFF:FFFFFFF:F,FF,FNM:i:1MD:Z:6T144MC:Z:26S125MAS:i:146XS:i:27Yt:Z:RU A00126:224:H7HMWDSX3:3:1115:6686:273832193Test_seq1664454827M124H=166310-162TTATCCCGGAAACCTCTTTGCTTTTAGFFFFFFFFFFFFFFF::,FFFFFFFFFNM:i:0MD:Z:27MC:Z:151MAS:i:27XS:i:0SA:Z:Test_seq,166550,-,26S125M,60,3;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1115:6686:27383145Test_seq1665506026S125M=166310-365TTATCCCGGAAACCTCTTTGCTTTTAGATCAATAAAAAACCCTCTCCCTCCCTTCCTCCCTCCCCCCTTTGTAACCACAAAAGTATGTGTTCTTCTCCGTTTTTACTATTTCTCAAGATCTTATAATAGTGGTCTTATACAATATTTGTCCFFFFFFFFFFFFFFF::,FFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFFFFFFF:FFFFFFNM:i:3MD:Z:27C50T9C36MC:Z:151MAS:i:110XS:i:63SA:Z:Test_seq,166445,-,27M124S,48,0;Yt:Z:RU 60 60 CCCAGGCGCCACAATTCAAAGTGGGATGCAGAATGTTTTCATAATAGAATTATTTTGCCCGTTGACTTAGAAGTCTCCTCAAACCATGTTCCCCAGACCCCAGCCCCTGCTCCGCTGACCTTTGAAGCTTTCATTTTATCCCGGAAACCTC TTATCCCGGAAACCTCTTTGCTTTTAGATCAATAAAAAACCCTCTCCCTCCCTTCCTCCCTCCCCCCTTTGTAACCACAAAAGTATGTGTTCTTCTCCGTTTTTACTATTTCTCAAGATCTTATAATAGTGGTCTTATACAATATTTGTCC +A00126:224:H7HMWDSX3:3:1115:27516:27915 Test_seq 169316 Test_seq 169778 + - UU A00126:224:H7HMWDSX3:3:1115:27516:27915161Test_seq16931660151M=169628463TAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATGTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFFFF:F,FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF,F:FFFFF,FFFF,FFFF::FFFFFF,FFF,FFNM:i:1MD:Z:37T113MC:Z:151MAS:i:146XS:i:107Yt:Z:UU A00126:224:H7HMWDSX3:3:1115:27516:2791581Test_seq16962860151M=169316-463CGTTGCATTCTATGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTATTGTTCATTTTCCAAGTATTTGATTTCTTTTCCCTAGTTTTTCTGTTATTGATCTCTAGTTTTATTGCCTTGTGGTCTGAFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFNM:i:8MD:Z:0T5T5A22G83C4T5C2C17MC:Z:151MAS:i:115XS:i:0Yt:Z:UU 60 60 TAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATGTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGG CGTTGCATTCTATGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTATTGTTCATTTTCCAAGTATTTGATTTCTTTTCCCTAGTTTTTCTGTTATTGATCTCTAGTTTTATTGCCTTGTGGTCTGA +A00126:224:H7HMWDSX3:3:1115:31584:28040 ! 0 Test_seq 167925 - + NR A00126:224:H7HMWDSX3:3:1115:31584:2804081Test_seq16821860128M23S=167925-421TCTTTTTGTTGATGTAGAGGAATCCAGGTGATTTTTGTATGTTTATTTTATAACCTGAGACTCTTCCAAACTCTTCTATTACTTTCAGTAGTTCTCTGGAGGATTCCTTAGGGTTTTCCATGTATACGATCAATCATTTCTTCATTTGCTC:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:4MD:Z:26A26T27G11T34MC:Z:151MAS:i:108XS:i:31Yt:Z:NR A00126:224:H7HMWDSX3:3:1115:31584:28040161Test_seq16792560151M=168218421AGTGATTTGTTTCTCTATCCCCTTAAAATATGACATTGGTATTTGGATTGGAAGTGCGTTATATGTATAGATGGCTTTTGGTAGAATAGACATTTTTACTATGTTAAGTCTTCCTATCCATGAGCAGGGTATGTTTTTCCACTTAAGTATGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FF,FFFFFFFFFFFFFFFFF:FFNM:i:1MD:Z:135C15MC:Z:128M23SAS:i:146XS:i:73Yt:Z:NR 0 60 AGTGATTTGTTTCTCTATCCCCTTAAAATATGACATTGGTATTTGGATTGGAAGTGCGTTATATGTATAGATGGCTTTTGGTAGAATAGACATTTTTACTATGTTAAGTCTTCCTATCCATGAGCAGGGTATGTTTTTCCACTTAAGTATG +A00126:224:H7HMWDSX3:3:1115:15944:29731 Test_seq 166867 Test_seq 167319 + - UU A00126:224:H7HMWDSX3:3:1115:15944:2973197Test_seq16686738151M=167169453GCTGCAATAAACATGGGTGTGCATATATCTGTTTGTATGAAGGCTCTTGTATCTCTAGGGTCTATTCCTAGGAGTGGGATTTCTGGGTTGTATGGTAGTTCTATTTCTAACTGTTTAAGATAACGCCAGATGGATTTCCAAAGTGGTTGTAFFFFF,,FFFFFF:FFF:FFFFF:FFFFFFFF,FFF:FFF:FFF:F::FFFFFFF::FFF,,::FFFFFFFFFFFFFF:,::F:FFF,FFF,,FF,,FFFFF,FFFFFFFFFFF,FFFFFFF,F:FF:FFFFFFF:FFF::FFFFFFFFF,NM:i:5MD:Z:26G9G13T10A8T80MC:Z:151MAS:i:126XS:i:106XA:Z:Test_seq,-214017,151M,9;Yt:Z:UU A00126:224:H7HMWDSX3:3:1115:15944:29731145Test_seq16716952151M=166867-453TAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAATCATGTAGATTTTAGAGATC:FF,FF,F:FFF:FFFF,FFFFF,FF:FF:FFFFFFFFFF::FF:FFF:FFF:F,FFFFFF,F:FFFFFFF:FFFFFFFFFFFFF,FFFFFF:FFFF:FFF,FFFFFFFFFFFFFFFF:FFFFF:FFFFFFFF:,FFFFFFFF:FFFFFFFNM:i:1MD:Z:74T76MC:Z:151MAS:i:146XS:i:121XA:Z:Test_seq,+213715,151M,6;Yt:Z:UU 38 52 GCTGCAATAAACATGGGTGTGCATATATCTGTTTGTATGAAGGCTCTTGTATCTCTAGGGTCTATTCCTAGGAGTGGGATTTCTGGGTTGTATGGTAGTTCTATTTCTAACTGTTTAAGATAACGCCAGATGGATTTCCAAAGTGGTTGTA TAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAATCATGTAGATTTTAGAGATC +A00126:224:H7HMWDSX3:3:1115:19054:30639 ! 0 Test_seq 168458 - + NR A00126:224:H7HMWDSX3:3:1115:19054:30639145Test_seq16859060126M25S=168458-258CTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGGACTTTGTCAAATGCCTTTTCTGCATCAATTGATAAGATCATGTGGTTTTTGTCTTTTGTTTTATTTATGTGATGGATTACATTAGATCTGCTGTGAGTAGCCAAFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:3MD:Z:46A26T23A28MC:Z:151MAS:i:111XS:i:81Yt:Z:NR A00126:224:H7HMWDSX3:3:1115:19054:3063997Test_seq16845860151M=168590258ATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF,FF:FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:37T113MC:Z:126M25SAS:i:146XS:i:91Yt:Z:NR 0 60 ATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGT +A00126:224:H7HMWDSX3:3:1115:17933:33301 ! 0 Test_seq 171866 - - NR A00126:224:H7HMWDSX3:3:1115:17933:3330197Test_seq1714676029S122M=171716400AATGAAGAAACCTTAAGAATCATGTGGGACTTAGGGTTTCTTCATTTTTTTAAATTCTTTTATCTGATTTTTTTTCAGCTATGTTGGAGTTGATTCCCTGGTCCTCCAGAAGTCCCAGTCTACATTCTAATTGCTCGAGTCAGCTCCTCTGFFF:FFF:FFFFFFF:F,F,FFFFFFF,FF,:FF::F::FFFFF,:FF:,FF:FF::,,FF:,F::FFFFFF::F,F::FF::FFF,F,FFF:FF,,F,,FF,F,,:F,FFFFFF:F:F,FFF:,F,,FFF,,,,FFFFFF,:FF,,,F:,NM:i:3MD:Z:22T89T3T5MC:Z:151MAS:i:107XS:i:43Yt:Z:NR A00126:224:H7HMWDSX3:3:1115:17933:33301145Test_seq17171660151M=171467-400TCTTCAACAGTTTTATCAGTGTGTTCCTTGGCTTTTTCTGCAGTTATCCTAATTTCATTTGTGATATCTTTAAGCATTCTGTAAATTAGTTTTTTATATTCTGTATCTGATAATTCCAGGATTGTATCTTCATTTGGGAAAGATTTTGATTFFF:FFFFFFFFFFFFFFFFFFFFFFF::FFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:1T149MC:Z:29S122MAS:i:149XS:i:30Yt:Z:NR 0 60 TCTTCAACAGTTTTATCAGTGTGTTCCTTGGCTTTTTCTGCAGTTATCCTAATTTCATTTGTGATATCTTTAAGCATTCTGTAAATTAGTTTTTTATATTCTGTATCTGATAATTCCAGGATTGTATCTTCATTTGGGAAAGATTTTGATT +A00126:224:H7HMWDSX3:3:1115:4517:33583 ! 0 Test_seq 116372 - + NR A00126:224:H7HMWDSX3:3:1115:4517:3358381Test_seq11672460127M24S=116372-479CTCTGCTTCCAGCCAAGATCCATCTGACATCAGCAATGATATGCCTTGTTCCACTTCCTCTTCTGAATCTGGCTTGGATTTCTGGCAGTTCCCTGTCAGTGTACTGCTGCAACATTTTTGAATGATCGATCCACAAATCGGCATATCATAGFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFF,FFFFFFFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF,F,F:FFFFFFFFFFFFFFNM:i:1MD:Z:19T107MC:Z:151MAS:i:122XS:i:45Yt:Z:NR A00126:224:H7HMWDSX3:3:1115:4517:33583161Test_seq11637260151M=116724479GCTTACATTTGTAAGAATATAGTAGTAAGTAGGAGAGAGAGAACAGCACACCTGATGCTGAGCTCTTCTTCATGTAGTCCAGCTTCTTGGAGTATTTGCTCAGTGTACAAATTGACTAAGTATGGTGAAAGGATACAACCCTGACACACACFFFFF,FFFFFFFFFF:FFFFFFFFFFF:FFF:F:FFFFFFFFFF:FFFFFFFF,FFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF:FF,FF:FFFFFF,F:FFFFF,FFFFFFFFFFFFFFFF:FFF:FFFFFFFNM:i:1MD:Z:7C143MC:Z:127M24SAS:i:146XS:i:42Yt:Z:NR 0 60 GCTTACATTTGTAAGAATATAGTAGTAAGTAGGAGAGAGAGAACAGCACACCTGATGCTGAGCTCTTCTTCATGTAGTCCAGCTTCTTGGAGTATTTGCTCAGTGTACAAATTGACTAAGTATGGTGAAAGGATACAACCCTGACACACAC +A00126:224:H7HMWDSX3:3:1115:31313:33771 ! 0 Test_seq 38503 - + NR A00126:224:H7HMWDSX3:3:1115:31313:3377181Test_seq3876660124M27S=38503-387AACTGCTACAATCAACCAAATTGACCTCATAGACTTATACAGAACACTCCACCCAACAGCTGCAAAGTATAAGTTTTTTCTAGTACACATGGAACATTCTCTAGAATAGATCACATATTAGATCGATCAACCTCCACCACTTCATTCTGAA,F:FFF,F:F:FF:FFF:,F,FFFF:FF,FFFFFF:F,FFFFFFF:FFFFFFF:,,::FFFF:,,,FFFF:FFFFFFFFFFFFFFF:FF,FFF,F::FFF:FFFFFF:FFFFF:FFFFFFF:FFFFFFF,FFFFF:FF,FF:FFFFFFFFFNM:i:0MD:Z:124MC:Z:151MAS:i:124XS:i:29Yt:Z:NR A00126:224:H7HMWDSX3:3:1115:31313:33771161Test_seq3850360151M=38766387AATTTCTGACAGAATAGACTTTAAAGTTAAATCCACCACAAAGGATAAAGAAGGACACTACATAATGATTAAAGGGACAACTGGTCAGGAAGATATAACCATATTAAGTATTTATGCACCCAATGACAGGGCTGCAAGATTCATAAAACAAF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFF:FFFFFFF:F:FF:FF:FFFF,FFFFF:FFF,F:FF:FF,FF:FF:FFFFFFFFFF::FFFFFFFFFFFFFFFFFFF:FF::FF:FF:NM:i:0MD:Z:151MC:Z:124M27SAS:i:151XS:i:31Yt:Z:NR 0 60 AATTTCTGACAGAATAGACTTTAAAGTTAAATCCACCACAAAGGATAAAGAAGGACACTACATAATGATTAAAGGGACAACTGGTCAGGAAGATATAACCATATTAAGTATTTATGCACCCAATGACAGGGCTGCAAGATTCATAAAACAA +A00126:224:H7HMWDSX3:3:1115:23113:33974 Test_seq 171813 Test_seq 172171 + - UU A00126:224:H7HMWDSX3:3:1115:23113:33974161Test_seq17181360151M=172038359ATTCTGTATCTGATAATTCCAGGATTGTATCTTCATTTGGGAAAGATTTTGATTCTTTTGTTTGGGGGGTTGGAGAAGCTGTCATGGTCTGTTTCTTTATGTGGTTTGATACGGACTGCTGTCTCCGAGCCATCACTGGGAAACTAGATTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFNM:i:1MD:Z:111T39MC:Z:4S71M1I63M12SAS:i:146XS:i:40Yt:Z:UU A00126:224:H7HMWDSX3:3:1115:23113:3397481Test_seq172038604S71M1I63M12S=171813-359TTCCGTCTCTAAGTCTGTGTTTGTTGTTCAGAGTTCGTAGATTGTTATGTATGTGATCGATTCACTTGTTTTTCCTGAGTCTTTGTTGCAAGAGGGATCCGCGGTAGCGTCCACCTAGTCCGCCATCTTGGCCCCGCCTACAGTACTAGCAFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF,FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFNM:i:3MD:Z:44A74G14MC:Z:151MAS:i:117XS:i:0Yt:Z:UU 60 60 ATTCTGTATCTGATAATTCCAGGATTGTATCTTCATTTGGGAAAGATTTTGATTCTTTTGTTTGGGGGGTTGGAGAAGCTGTCATGGTCTGTTTCTTTATGTGGTTTGATACGGACTGCTGTCTCCGAGCCATCACTGGGAAACTAGATTT TTCCGTCTCTAAGTCTGTGTTTGTTGTTCAGAGTTCGTAGATTGTTATGTATGTGATCGATTCACTTGTTTTTCCTGAGTCTTTGTTGCAAGAGGGATCCGCGGTAGCGTCCACCTAGTCCGCCATCTTGGCCCCGCCTACAGTACTAGCA +A00126:224:H7HMWDSX3:3:1115:12762:34491 Test_seq 224240 Test_seq 380554 - + RU A00126:224:H7HMWDSX3:3:1115:12762:34491145Test_seq22409060151M=380554156315TGTATGCATCTATTGTTGTAAATAGACCTCTGAGCACTGCCTTTGCTGTATCCCAATGGTTTTAGTATGATGTGTTTTCATTCTCGGAATTTTTTTTATTCCATCTTTGATTTCTTTTATTACGCAGTGGTTTTCAAGCAGGGTGTTACTCFFFFFF:F:F,::F:,FFFFFFFFFF:FFFFFFFF:FFFFFFFFF:FF,FFFFF,FF,,F:FF:F::FF::FFFFFF:FFFFFF,FFFF,FFFFFF:F,,FFFFFFF:FFFFFFF:FFFFFFF::FFFFFFFFFF,:FFFFFFF:FFFFFFNM:i:0MD:Z:151MC:Z:23M128SAS:i:151XS:i:37Yt:Z:RU A00126:224:H7HMWDSX3:3:1115:12762:3449197Test_seq3805542423M128S=224090-156315TTGGTTAGCAGCTTGAGCTCTTAGATCCTTTTTATTTCAGTTGGGTCTCTAGTAATGGCCCCCATTTCATTTCTTATTTGGGTTGGTTGTGTCCTCTACTTTTTTCTTTTATCAGTTTTGTCAGTGGTTTGTTGTTTTTGTTGATCCTTTCFFFF,F,FF:FF:,,FF:F,::F::FF:FFFFF:FF,FF:F:,F:FFF:F,F:FFF,,F:FF,:,F,F:::F::F:FF,:,,:F,,FF:F:F,,,,F,,,F:F,::F,,FFF,,F,FFFF::F,F,,,::FF,,,FFFFFF,,FF,,F,:FNM:i:0MD:Z:23MC:Z:151MAS:i:23XS:i:0SA:Z:Test_seq,223789,+,23S128M,60,5;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1115:12762:344912145Test_seq2237896023H128M=224090452GATCCTTTTTATTTCAGTTGGGTCTCTAGTAATGGCCCCCATTTCATTTCTTATTTGGGTTGGTTGTGTCCTCTACTTTTTTCTTTTATCAGTTTTGTCAGTGGTTTGTTGTTTTTGTTGATCCTTTC::FF:FFFFF:FF,FF:F:,F:FFF:F,F:FFF,,F:FF,:,F,F:::F::F:FF,:,,:F,,FF:F:F,,,,F,,,F:F,::F,,FFF,,F,FFFF::F,F,,,::FF,,,FFFFFF,,FF,,F,:FNM:i:5MD:Z:27T33A0T11C36A16MC:Z:151MAS:i:103XS:i:0SA:Z:Test_seq,380554,+,23M128S,24,0;Yt:Z:RU 60 24 TGTATGCATCTATTGTTGTAAATAGACCTCTGAGCACTGCCTTTGCTGTATCCCAATGGTTTTAGTATGATGTGTTTTCATTCTCGGAATTTTTTTTATTCCATCTTTGATTTCTTTTATTACGCAGTGGTTTTCAAGCAGGGTGTTACTC TTGGTTAGCAGCTTGAGCTCTTAGATCCTTTTTATTTCAGTTGGGTCTCTAGTAATGGCCCCCATTTCATTTCTTATTTGGGTTGGTTGTGTCCTCTACTTTTTTCTTTTATCAGTTTTGTCAGTGGTTTGTTGTTTTTGTTGATCCTTTC +A00126:224:H7HMWDSX3:3:1115:29568:35258 Test_seq 166063 Test_seq 166378 + - UU A00126:224:H7HMWDSX3:3:1115:29568:35258161Test_seq16606360151M=166228316TCCCATTGGTCTCCTGCTCCCTCAGGAGTTGTCTGTTGTGCTCCCTGACAGGGCAGACATCGATTGTGGCCGGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCTTAGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:FNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:57Yt:Z:UU A00126:224:H7HMWDSX3:3:1115:29568:3525881Test_seq16622860151M=166063-316TGGTGTTCTTCCTTTGCCTTTGCTCCAGGTGGGTTGAGACCAATTGATGTATCTTAGATGGCCGCTTGTTGGCATTTAGGACCCCAGGTGCCACAATTCAAAGTGGGATGCAGAGTGTTTTCATAATAGAATTGTTTTGCCCATTGACTTA:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFNM:i:3MD:Z:114A18A8G8MC:Z:151MAS:i:136XS:i:76Yt:Z:UU 60 60 TCCCATTGGTCTCCTGCTCCCTCAGGAGTTGTCTGTTGTGCTCCCTGACAGGGCAGACATCGATTGTGGCCGGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCTTAG TGGTGTTCTTCCTTTGCCTTTGCTCCAGGTGGGTTGAGACCAATTGATGTATCTTAGATGGCCGCTTGTTGGCATTTAGGACCCCAGGTGCCACAATTCAAAGTGGGATGCAGAGTGTTTTCATAATAGAATTGTTTTGCCCATTGACTTA +A00126:224:H7HMWDSX3:3:1115:16432:35305 ! 0 Test_seq 167861 - + NR A00126:224:H7HMWDSX3:3:1115:16432:35305145Test_seq1681528118M33S=167861-409CTAAGTATTTTATCTTCTTGGGGGCTACTGTGAATGGTATTGATTTGGTTATGTCCTCTTCGGTGTTCTTTTTGTTGATGTAGAGGAATCCAAGTGATTTTTGTATGTTTATTTTATATCCTGAGACTACTCATGCCCAAAGAATGCCGAGFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:,F,FFFFFFFFFF:FFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFNM:i:2MD:Z:17C34T65MC:Z:151MAS:i:108XS:i:103XA:Z:Test_seq,+212759,23S128M,5;Yt:Z:NR A00126:224:H7HMWDSX3:3:1115:16432:3530597Test_seq16786160151M=168152409CTTTCTTCTTCTTTTTCAGTAATGCTTTGCTTATCCGGGGGTTCTTTCCCTTCCATATGAAATTAGTGATTAGTTTCTCTATCCCCTTAAAATATGACATTGGTATTTGGATTGGAAGTGCGTTATATGTATAGATGGCTTTTGGTAGAATFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFF:FFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFF:F:F:FFNM:i:1MD:Z:71T79MC:Z:118M33SAS:i:146XS:i:52Yt:Z:NR 0 60 CTTTCTTCTTCTTTTTCAGTAATGCTTTGCTTATCCGGGGGTTCTTTCCCTTCCATATGAAATTAGTGATTAGTTTCTCTATCCCCTTAAAATATGACATTGGTATTTGGATTGGAAGTGCGTTATATGTATAGATGGCTTTTGGTAGAAT +A00126:224:H7HMWDSX3:3:1115:2672:36401 Test_seq 169299 Test_seq 169663 + - UU A00126:224:H7HMWDSX3:3:1115:2672:3640197Test_seq16929960151M=169513365CCTTTCCTGTTTTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTT:FFFFF,,,FFFFF,FFFFFFFFFFFFFF:FFFF,:FFFFF:FFFFFFFFFFFFFFFFFFFF::FF:,F::FFFF,,,FFFFFF::FF,F:F:F,FF:FFFFF:FFFF,F:FF,FF,FFFF,FFF,,:,F:FF,FF:FF:FFFFFF:FFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:101Yt:Z:UU A00126:224:H7HMWDSX3:3:1115:2672:36401145Test_seq16951360151M=169299-365TCTCTGATTTTGGCTCTTTCTTCTTTTTGTATGTGTGCATTTATCGATATAAATTGGCCTCTGAGCACTGCCTTTGCTGTGTCCCAGAGGTTTTGATAGGAAGTATTTTCATTCTCGTTGCTTTCTAAGAATTTCCTTATTCCCTCCTTGAFFFFFFFFFFFFF,FFFFFFFFF:FFFFFFFFFFFFFF::FFFFF:FFF:FFFFFFF,FFF::FFF,FFFFF,FFFFFFFFF:FFFF:FF::F:FFFF,::FFFF:FFFFFF::F:FFFFFFFF,FFF:FFFFFFFFFFFFFF:F,F,,:FNM:i:5MD:Z:6C0C63T43T34G0MC:Z:151MAS:i:132XS:i:33Yt:Z:UU 60 60 CCTTTCCTGTTTTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTT TCTCTGATTTTGGCTCTTTCTTCTTTTTGTATGTGTGCATTTATCGATATAAATTGGCCTCTGAGCACTGCCTTTGCTGTGTCCCAGAGGTTTTGATAGGAAGTATTTTCATTCTCGTTGCTTTCTAAGAATTTCCTTATTCCCTCCTTGA +A00126:224:H7HMWDSX3:3:1116:21097:1329 Test_seq 167966 Test_seq 212718 + + UU A00126:224:H7HMWDSX3:3:1116:21097:132965Test_seq16796621151M=21271844753TTTGGATCGGAATTGCATTGTATGTATAGATGGCTTTTGGTAGAATAGACATTTTGACTATGTTAAGTCTTCCTATCCATGAGCAAGGTATGTTTTTCCACTTAAGTATGTCCTTTTGAATTTCTTGTACTAGAGCTTTGTAGTTTTCTTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFNM:i:11MD:Z:7T4G3G2A35T29G8C34G0C4T3A11MC:Z:151MAS:i:96XS:i:83XA:Z:Test_seq,-212922,151M,14;Yt:Z:UU A00126:224:H7HMWDSX3:3:1116:21097:1329129Test_seq21271831151M=167966-44753TCCTCCAGAAAACTACTGAAACTAATAGAAGAGTTTGGCAGAGTCTCAGGTTATAAGATAAACATACAAAAATCACTTGGATTCCTCTACATCAACAAAAAGAACATCGAAGAGGAAATAACCAAATCAATACCATTCACAGTAGCCCCCAFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:108A42MC:Z:151MAS:i:146XS:i:131XA:Z:Test_seq,-168170,151M,4;Yt:Z:UU 21 31 TTTGGATCGGAATTGCATTGTATGTATAGATGGCTTTTGGTAGAATAGACATTTTGACTATGTTAAGTCTTCCTATCCATGAGCAAGGTATGTTTTTCCACTTAAGTATGTCCTTTTGAATTTCTTGTACTAGAGCTTTGTAGTTTTCTTT TCCTCCAGAAAACTACTGAAACTAATAGAAGAGTTTGGCAGAGTCTCAGGTTATAAGATAAACATACAAAAATCACTTGGATTCCTCTACATCAACAAAAAGAACATCGAAGAGGAAATAACCAAATCAATACCATTCACAGTAGCCCCCA +A00126:224:H7HMWDSX3:3:1116:9525:1548 Test_seq 165792 Test_seq 166199 + - UU A00126:224:H7HMWDSX3:3:1116:9525:1548161Test_seq16579260110M2D41M=166049408CCGTCTTCCCTCTCTCTCCATCTTCCCATCCCTCCTCCAGTCAAGAGTTGCCAACACACTCTCCCGTGTCCACCTGATTTAATTAGCTCACTCTTCCTCAGCATCTCTCTCCCCCCCACTGACCAGTCCTTTTCATGCCTGATGAGTTGTCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:12MD:Z:2A5T9T13C31A10A8G11A13^CC7G19T13MC:Z:151MAS:i:95XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1116:9525:154881Test_seq16604960151M=165792-408ATTTGGGGTCTGTATCCCATTGGTCTCCTGCTCCCTCAGGAGTTGTCTGTTGTACTCCCTGACAGGGCAGACATCGATTGTGGCCGGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:53G97MC:Z:110M2D41MAS:i:146XS:i:50Yt:Z:UU 60 60 CCGTCTTCCCTCTCTCTCCATCTTCCCATCCCTCCTCCAGTCAAGAGTTGCCAACACACTCTCCCGTGTCCACCTGATTTAATTAGCTCACTCTTCCTCAGCATCTCTCTCCCCCCCACTGACCAGTCCTTTTCATGCCTGATGAGTTGTC ATTTGGGGTCTGTATCCCATTGGTCTCCTGCTCCCTCAGGAGTTGTCTGTTGTACTCCCTGACAGGGCAGACATCGATTGTGGCCGGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTC +A00126:224:H7HMWDSX3:3:1116:28917:2190 Test_seq 74270 Test_seq 74579 + - UU A00126:224:H7HMWDSX3:3:1116:28917:219097Test_seq7427060151M=74429310TGGGAGTCCAGGGAAGCCTTCTGGAGACCAAATCTGTTACCATCCGACTCATAGTGACCCTATAGGACAGAATAGAACTCCCCCATAGGGTTTCCAAGGCTGTAAACCTTTACAGAAGCAGATTGCCATGTCTTTCTCCCACGAAGCAGCTFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:41Yt:Z:UU A00126:224:H7HMWDSX3:3:1116:28917:2190145Test_seq7442960151M=74270-310CGAACCGCGGACCTTTCAGTCAGCAGCCGAGTGCTTCACCACCGTACCACCAGGGCTCCCCTCCTGCGGAAAGTGATGCATAAACTGAAATCTGACCAATACATAGACTCAGCCCCCAGGATAAGAGGAACAAGAGCAGGAGTGTTCTAGGFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:F:,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU 60 60 TGGGAGTCCAGGGAAGCCTTCTGGAGACCAAATCTGTTACCATCCGACTCATAGTGACCCTATAGGACAGAATAGAACTCCCCCATAGGGTTTCCAAGGCTGTAAACCTTTACAGAAGCAGATTGCCATGTCTTTCTCCCACGAAGCAGCT CGAACCGCGGACCTTTCAGTCAGCAGCCGAGTGCTTCACCACCGTACCACCAGGGCTCCCCTCCTGCGGAAAGTGATGCATAAACTGAAATCTGACCAATACATAGACTCAGCCCCCAGGATAAGAGGAACAAGAGCAGGAGTGTTCTAGG +A00126:224:H7HMWDSX3:3:1116:3495:3724 Test_seq 335700 Test_seq 436771 - - UR A00126:224:H7HMWDSX3:3:1116:3495:37242209Test_seq4364016032H119M=436621371GATCTTTTTTCCACATTGTGAAAAGTGAAACTATGAAAAATCTATACTTTTTCTTATTTTCCTCCTTTAGAAAATCATATTTTTCTTCCAAAAGGTAAAACACATTTGAACACCAGTTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFF:FFFFFFF:FFF,F:FF:FFF:FFF,FFFF:FF,FFFF,FFFFF:FFFFFFFFFFFFF,FFFFF:FF:FFNM:i:0MD:Z:119MC:Z:151MAS:i:119XS:i:0SA:Z:Test_seq,335669,-,119S32M,60,0;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1116:3495:3724177Test_seq33566960119S32M=436621101072AAACTGGTGTTCAAATGTGTTTTACCTTTTGGAAGAAAAATATGATTTTCTAAAGGAGGAAAATAAGAAAAAGTATAGATTTTTCATAGTTTCACTTTTCACAATGTGGAAAAAAGATCGATCTATCCTGAAGTACCTTAAGGTTAGTGGAFF:FF:FFFFF,FFFFFFFFFFFFF:FFFFF,FFFF,FF:FFFF,FFF:FFF:FF:F,FFF:FFFFFFF:FFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:32MC:Z:151MAS:i:32XS:i:0SA:Z:Test_seq,436401,+,32S119M,60,0;Yt:Z:UR A00126:224:H7HMWDSX3:3:1116:3495:3724113Test_seq43662160151M=335669-101072AAAAATGCCACGTTGGATTAACCTTGACTCAACTGGACATGTAGATACCCCATGTGGATGGTTAGAGGGGTGGCAAGGTCCATCCTCCCCAAAGAGACTGTATCCCTACCTGCCCGAGGACCTGAAGTGGTTACGTGTCCAACCAGTTGCCFFFFFFFFFFFFF,FFFFFFFFFFFFFFFF:FFFFFFF:FFFFFF:FFFFFFFFFFFF:FFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF::FFFFFFFFFFF:FFFFFFFFFNM:i:0MD:Z:151MC:Z:119S32MAS:i:151XS:i:0Yt:Z:UR 60 60 AAACTGGTGTTCAAATGTGTTTTACCTTTTGGAAGAAAAATATGATTTTCTAAAGGAGGAAAATAAGAAAAAGTATAGATTTTTCATAGTTTCACTTTTCACAATGTGGAAAAAAGATCGATCTATCCTGAAGTACCTTAAGGTTAGTGGA AAAAATGCCACGTTGGATTAACCTTGACTCAACTGGACATGTAGATACCCCATGTGGATGGTTAGAGGGGTGGCAAGGTCCATCCTCCCCAAAGAGACTGTATCCCTACCTGCCCGAGGACCTGAAGTGGTTACGTGTCCAACCAGTTGCC +A00126:224:H7HMWDSX3:3:1116:1886:5885 Test_seq 133235 Test_seq 133657 + - UU A00126:224:H7HMWDSX3:3:1116:1886:588597Test_seq13323560151M=133507423AATCATGGTCCAGCCAAGTTGGCACATAAGGTTAACAATCATAGCCAGCGTTACTCTTGCCTTGCACCTCGGTGTTCGTTACTGCCAGATGTACGTCGTTAATGTGCGTAACAGTCAGATACGAGATTTTTTACTATTGTCATGAACGTAAFFFFFFF:FFFF,FFFFFFF,FFFFF:FF,FFFF::,FFFFFFFFFF,F:FFFFFFFFF,FFFFFF,FFF,FFFFFF,FFFFF,FFFFFFFFFF:FFFFFFFFFF:::FFF,FFF:F,FFFF,F:FFFFFF:F,FFFF,F,FF::F,FFFFNM:i:1MD:Z:36C114MC:Z:151MAS:i:146XS:i:0Yt:Z:UU A00126:224:H7HMWDSX3:3:1116:1886:5885145Test_seq13350760151M=133235-423GGGGTTTCACTGTCTGGTTTTTAGAAGCAGCATGCCAGGTCTCTGTTCCCTAGTCCACCTTAGTCTGGACACTCCGCTGAAACCTGTCCAGTGTCATAGCGACACACAGGCCTACACTGAGAGACAAGTGGTGGATGAATTTGAAGTACGT:F::FFFFFFFF,FF,FFF,,F:FFF,FFFFFF,FFF,:F:F::FFFF,FFF:F,:FFFFFFFF:,,F,:FF:FFFFFFFFFFFFFF:FFFF:F:FFFFF::FFFFFFFFF,F,FFFFFFFFFF,FFFFFFFFF,FF,F:FFFFFFFFF,FNM:i:5MD:Z:12G29T70C20C2C13MC:Z:151MAS:i:126XS:i:0Yt:Z:UU 60 60 AATCATGGTCCAGCCAAGTTGGCACATAAGGTTAACAATCATAGCCAGCGTTACTCTTGCCTTGCACCTCGGTGTTCGTTACTGCCAGATGTACGTCGTTAATGTGCGTAACAGTCAGATACGAGATTTTTTACTATTGTCATGAACGTAA GGGGTTTCACTGTCTGGTTTTTAGAAGCAGCATGCCAGGTCTCTGTTCCCTAGTCCACCTTAGTCTGGACACTCCGCTGAAACCTGTCCAGTGTCATAGCGACACACAGGCCTACACTGAGAGACAAGTGGTGGATGAATTTGAAGTACGT +A00126:224:H7HMWDSX3:3:1116:5041:6715 Test_seq 168462 Test_seq 168810 + - UU A00126:224:H7HMWDSX3:3:1116:5041:671597Test_seq16846260151M=168674349GAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFFFFF::F:F:F,FFFFFFFFFFFF:F:F:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF,FFF:FFFFFF:FF:FFFF,FFFFFFFF:FFF,F:,FFFFF:FFFNM:i:1MD:Z:33T117MC:Z:137M14SAS:i:146XS:i:91Yt:Z:UU A00126:224:H7HMWDSX3:3:1116:5041:6715145Test_seq16867460137M14S=168462-349TCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTCTTTTGATATTGAACCAGCCTTGCATACCTGATATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTGATCTCCGGGACTTF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FF:F:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:5MD:Z:42T10T2C7A19G52MC:Z:151MAS:i:112XS:i:30Yt:Z:UU 60 60 GAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGA TCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTCTTTTGATATTGAACCAGCCTTGCATACCTGATATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTGATCTCCGGGACTT +A00126:224:H7HMWDSX3:3:1116:31720:7545 Test_seq 303522 Test_seq 305328 + + RU A00126:224:H7HMWDSX3:3:1116:31720:7545129Test_seq30352260151M=3053281807ATCTTTTATTTGGGAGAAACAAGTCAGAATTAATTCCTGTTGATTGCACTGAATTATAAATGATGAAGAATCAGAAATCAGCTACTGGCCCACAGGCATGTTGCAGAAACTCAATAAATGTTAGCCATCATCTCCTTTTTCATCATCATAAFFFFFFF:FFFF:F:FFFFFFFFFF:FFF:FFFF:FFFF,FFFFFFFFF,FFFFF::FFF:FFFFFFFFFFFFFFF,F:FFFFFFFFFF:FF:FF:FFFF,FFF:FFF:FFFFFF:FFFFFF,F:F,FFFFF:FFF:FFF:FFFF,FFF:FNM:i:0MD:Z:151MC:Z:20M131SAS:i:151XS:i:0Yt:Z:RU A00126:224:H7HMWDSX3:3:1116:31720:754565Test_seq305328620M131S=303522-1807CCATTGCTATCAAGTGGATTGATCACTCCCTGGAGAAGGACATCATGCTTGGTAAAGTAGAGGGCCAACATGGATTGACACAGTGGCTGCAACAATGGGCTCAAGCATAACAACAACTGTGAGGATGGCGCAGGGCTGGGCAGTGTTTCATFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFF:FFFFFFFFFFF:FF:FFF:FFFFF:F:FFFFFFFFF,F::FFFFFF:FFFFFFFFFFFFFFF,F:FFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFNM:i:0MD:Z:20MC:Z:151MAS:i:20XS:i:0SA:Z:Test_seq,303836,-,131M20S,60,0;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1116:31720:75452129Test_seq30383660131M20H=303522-445ATGAAACACTGCCCAGCCCTGCGCCATCCTCACAGTTGTTGTTATGCTTGAGCCCATTGTTGCAGCCACTGTGTCAATCCATGTTGGCCCTCTACTTTACCAAGCATGATGTCCTTCTCCAGGGAGTGATCFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFF:F,FFFFFFFFFFFFFFF:FFFFFF::F,FFFFFFFFF:F:FFFFF:FFF:FF:FFFFFFFFFFF:FFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:131MC:Z:151MAS:i:131XS:i:43SA:Z:Test_seq,305328,+,20M131S,6,0;Yt:Z:RU 60 6 ATCTTTTATTTGGGAGAAACAAGTCAGAATTAATTCCTGTTGATTGCACTGAATTATAAATGATGAAGAATCAGAAATCAGCTACTGGCCCACAGGCATGTTGCAGAAACTCAATAAATGTTAGCCATCATCTCCTTTTTCATCATCATAA CCATTGCTATCAAGTGGATTGATCACTCCCTGGAGAAGGACATCATGCTTGGTAAAGTAGAGGGCCAACATGGATTGACACAGTGGCTGCAACAATGGGCTCAAGCATAACAACAACTGTGAGGATGGCGCAGGGCTGGGCAGTGTTTCAT +A00126:224:H7HMWDSX3:3:1116:30870:8296 Test_seq 169295 Test_seq 169732 + - UU A00126:224:H7HMWDSX3:3:1116:30870:829697Test_seq16929560151M=169582438GTTTCCTTTCCTGTATTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFF:FFFFFFF:FFF:F:FFFFFFFF:F:F:FFFFFFFFFFF:FFFFFFF,FFFFF:FFFF:F,FFFFFFFFFFFFNM:i:1MD:Z:14T136MC:Z:151MAS:i:146XS:i:107Yt:Z:UU A00126:224:H7HMWDSX3:3:1116:30870:8296145Test_seq16958260151M=169295-438GCTTTTGCTGTGTCCCAGAGGTTTTGATAGGAAGTATTTTCATTCTCGTTGCTTTCTATGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTATTGTTCATTTTCCAAGTATTTGATTTCTTTTCCC:FFF:FFFFFFFFF,:FFF,FFFFF:FFFFFFF:FFFFFFFFFFFF,FFFF:FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:NM:i:3MD:Z:46T11A22G69MC:Z:151MAS:i:136XS:i:22Yt:Z:UU 60 60 GTTTCCTTTCCTGTATTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTG GCTTTTGCTGTGTCCCAGAGGTTTTGATAGGAAGTATTTTCATTCTCGTTGCTTTCTATGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTATTGTTCATTTTCCAAGTATTTGATTTCTTTTCCC +A00126:224:H7HMWDSX3:3:1116:5186:8719 ! 0 Test_seq 169344 - + NR A00126:224:H7HMWDSX3:3:1116:5186:871981Test_seq16961860129M22S=169344-403TTTTCATTCTCGTTGCTTTCTATGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTATTGTTCATTTTCCAAGTATTTGATTTCTTTTCCCTCGTTCTTCTGTTATTGATCGATCTGTCATACTCTGF:FFFFFFFFFFFF:FFFF,FFFFFFFFFFFFFF:FFFFF,FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFF:FFF:FFFFFFFFFFFNM:i:5MD:Z:10T11A22G70A3T8MC:Z:151MAS:i:104XS:i:0Yt:Z:NR A00126:224:H7HMWDSX3:3:1116:5186:8719161Test_seq16934460151M=169618403TTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGATTCTTTTGTTGCTCACTTTCTATTTGTFFFFFFFF:FFFFFFFFFFFFFFFFFFF:FFFFF,FFFFF:FFFF:FFFF,FF:FFFFFFFFFFFFFFF:FFFF,FFFFFFFFFFF,FFFFFFFF,FFF:FFFFFF,FFFFFFFFFFFFF:FFFFFFF,FFFF,F:FFFFF,FFFF,F:F,NM:i:1MD:Z:123G27MC:Z:129M22SAS:i:146XS:i:101Yt:Z:NR 0 60 TTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGATTCTTTTGTTGCTCACTTTCTATTTGT +A00126:224:H7HMWDSX3:3:1116:11080:9690 Test_seq 171531 Test_seq 171917 + - UU A00126:224:H7HMWDSX3:3:1116:11080:969097Test_seq17153160151M=171767387TTCCCTGGTCCTCCAGAAGTCCTAGTCTACATTCTAATTGCTCGAGTCTGCTCCTCTGACTTTCTATTGTGTTGTCAAATTCTATAATTTTATTGTTAATCTTTTGGATTTCTACATGCTGTCTCTCTATGTATTCTTGCAACTTATTAATFFFFFFF:F,FFFFFFFFFF::FFFFFFFFFF:FFFFFFF:FF:F:F,FFFFFFFF:F:::,FFFF:FFFFFFFFFFF,FF:FFF:F,:FFFFFFF:,FFFFFFF::FFF:FF:F,:F,,F:::F,:FFFFF,FF,FFF,,F,:FFFFF:FNM:i:6MD:Z:22C29T16C6T6G47G19MC:Z:151MAS:i:121XS:i:53Yt:Z:UU A00126:224:H7HMWDSX3:3:1116:11080:9690145Test_seq17176760151M=171531-387ATTTCATTTGTGATATCTTTAAGCATTCTGTAAATTAGTTTTTTATATTCTGTATCTGATAATTCCAGGATTGTATCTTCATTTGGGAAAGATTTTGATTCTTTTGTTTGGGGGGTTGGAGAAGCTGTCATGGTCTGTTTCTTTATGTGGTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFF,F:FFFFF::FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU 60 60 TTCCCTGGTCCTCCAGAAGTCCTAGTCTACATTCTAATTGCTCGAGTCTGCTCCTCTGACTTTCTATTGTGTTGTCAAATTCTATAATTTTATTGTTAATCTTTTGGATTTCTACATGCTGTCTCTCTATGTATTCTTGCAACTTATTAAT ATTTCATTTGTGATATCTTTAAGCATTCTGTAAATTAGTTTTTTATATTCTGTATCTGATAATTCCAGGATTGTATCTTCATTTGGGAAAGATTTTGATTCTTTTGTTTGGGGGGTTGGAGAAGCTGTCATGGTCTGTTTCTTTATGTGGT +A00126:224:H7HMWDSX3:3:1116:31367:10222 Test_seq 168346 Test_seq 168601 + - UU A00126:224:H7HMWDSX3:3:1116:31367:1022297Test_seq16834660130M=168472256ATCATGTCATCTGCAAATAGTGATAGCTTTACTTTGTCCTTACCAATCTGGATACCCTTTATTTTTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGTGGTGATAAAG*NM:i:7MD:Z:0G18A14C0C5G22C54C10MC:Z:130MAS:i:99XS:i:40Yt:Z:UU A00126:224:H7HMWDSX3:3:1116:31367:10222145Test_seq16847260130M=168346-256AAAGGGCATCCTTGTCTGGTTCCTGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTA*NM:i:0MD:Z:130MC:Z:130MAS:i:130XS:i:75Yt:Z:UU 60 60 ATCATGTCATCTGCAAATAGTGATAGCTTTACTTTGTCCTTACCAATCTGGATACCCTTTATTTTTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGTGGTGATAAAG AAAGGGCATCCTTGTCTGGTTCCTGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTA