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author | iuc |
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date | Mon, 04 Nov 2024 22:10:45 +0000 |
parents | 55b7b257e12f |
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<tool id="pbmm2" name="pbmm2" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="@PROFILE@"> <description>map PacBio reads to a reference genome.</description> <macros> <import>macros.xml</import> </macros> <expand macro="xrefs"/> <expand macro="requirements"/> <command detect_errors="exit_code"><![CDATA[ ## set up files #set reads_fn = 'reads.' + $reads.ext ln -s '$reads' '$reads_fn' && #if $reference_source.reference_source_selector == 'history': #set ref_fn = 'reference.fa' ln -f -s '$reference_source.ref_file' '$ref_fn' && #else: #set ref_fn = $reference_source.ref_file.fields.path #end if ## run pbmm2 pbmm2 align -j \${GALAXY_SLOTS:-4} #if str($output_options.l): -l $output_options.l #end if #if str($output_options.N): -N $output_options.N #end if --preset $preset '$reads_fn' '$ref_fn' out.aligned.bam --sort ]]></command> <inputs> <!-- from tools-iuc minimap2 wrapper --> <conditional name="reference_source"> <param name="reference_source_selector" type="select" label="Will you select a reference genome from your history or use a built-in index?"> <option value="cached">Use a built-in genome index</option> <option value="history">Use a genome from history and build index</option> </param> <when value="cached"> <param name="ref_file" type="select" label="Using reference genome" help="Select genome from the list"> <options from_data_table="all_fasta"> <filter type="sort_by" column="2" /> <validator type="no_options" message="No reference genomes are available" /> </options> <validator type="no_options" message="A built-in reference genome is not available for the build associated with the selected input file"/> </param> </when> <when value="history"> <param name="ref_file" type="data" format="fasta" label="Use the following dataset as the reference sequence" help="You can upload a FASTA sequence to the history and use it as reference" /> </when> </conditional> <param type="data" name="reads" format="fastq,fastq.gz,fasta,fasta.gz,bam" label="reads" help="PacBio reads in BAM or [gzipped] fasta or fastq format"/> <param argument="--preset" type="select" label="Set alignment mode"> <option value="CCS">PacBio CCS or HiFi reads</option> <option value="SUBREAD">PacBio CLR or raw subreads</option> <option value="ISOSEQ">PacBio IsoSeq transcripts</option> <option value="UNROLLED">Align entire raw ZMW</option> </param> <section name="output_options" title="Output Options" help="Sets -l, -N, --strip, --split-by-sample, --unmapped, --short-sa-cigar" expanded="False"> <param argument="-l" type="integer" min="0" label="Minimum mapped read length in basepairs" value="50" optional="true"/> <param argument="-N" type="integer" min="0" label="Output at maximum N alignments for each read, 0 means no maximum" value="0" optional="true"/> </section> </inputs> <outputs> <data name="bam" format="bam" from_work_dir="out.aligned.bam" label="${tool.name} on ${on_string} (BAM file)"/> </outputs> <tests> <!-- test1: basic test --> <test expect_num_outputs="1"> <param name="reference_source_selector" value="history" /> <param name="ref_file" ftype="fasta" value="bnd-ref.fasta"/> <param name="reads" value="bnd.bam"/> <output name="bam"> <assert_contents> <has_size value="2778" delta="200" /> </assert_contents> </output> </test> <!-- test2: map some reads for use with pbgcpp --> <test expect_num_outputs="1"> <param name="reference_source_selector" value="history" /> <param name="ref_file" ftype="fasta" value="All4mer.V2.01_Insert.fa"/> <param name="reads" value="out.aligned_subreads.bam"/> <param name="preset" value="SUBREAD"/> <output name="bam"> <assert_contents> <has_size value="538329" delta="50000" /> </assert_contents> </output> </test> <!-- test3: cached genome --> <test> <param name="reference_source_selector" value="cached" /> <param name="ref_file" value="bnd-ref"/> <param name="reads" value="bnd.bam"/> <output name="bam"> <assert_contents> <has_size value="2778" delta="200" /> </assert_contents> </output> </test> </tests> <help><![CDATA[ **What it does** A minimap2 wrapper for PacBio data: native PacBio data in ⇨ native PacBio BAM out. -------------- pbmm2 is Pacific Biosciences’ wrapper for `minimap2 <https://github.com/lh3/minimap2>`__\ ’s C API. It supports native PacBio input. Output can be used directly for polishing using `pbgcpp <root?tool_id=pbgcpp>`__. **Input**: PacBio reads in bam or [gzipped] fasta or fastq format. **NOTE**: to use the output of pbmm2 for pbgcpp (GenomicConsensus), you have to provide PacBio CLR reads in bam format. You can use pbmm2 to align other types of PacBio reads in [gzipped] fasta or fastq format, but you can’t use the output for pbgcpp (GenomicConsensus). **Output**: A **sorted** PacBio BAM file, suitable for use with pbgcpp (previously known as GenomicConsensus). ]]></help> <citations> <citation type="bibtex"> @misc{githubpbmm2, author = {PacBio}, year = {2023}, title = {pbmm2}, publisher = {GitHub}, journal = {GitHub repository}, url = {https://github.com/PacificBiosciences/pbmm2}, }</citation> </citations> <expand macro="creator"/> </tool>