# HG changeset patch # User iuc # Date 1708944919 0 # Node ID ca3ec50bfd94a6b48ce8dcdbfefebdfdddb97969 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/peakzilla commit defa1857cbd66d5f90e3e6f98dc11e1d215b742a diff -r 000000000000 -r ca3ec50bfd94 peakzilla.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/peakzilla.xml Mon Feb 26 10:55:19 2024 +0000 @@ -0,0 +1,160 @@ + + Identify transcription factor binding sites from ChIP-seq and ChIP-exo experiments + + 1.0 + 0 + + + python + peakzilla + + + + + + + '$results' + ]]> + + + + +
+ + + + + + +
+
+ + +
+
+ + + + log + + + negative + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + input.bam + samtools view -bS chip.sam > chip.bam + + # convert to BED format + bamToBed -i input.bam > input.bed + bamToBed -i chip.bam > chip.bed + + # run peakzilla + python peakzilla.py chip.bed input.bed > chip_peaks.tsv + + # Comparison of 2 datasets + # Determine significant peaks with a score threshold of 10 + python peakzilla.py -s 10 chip1.bed input1.bed > chip1_s10_peaks.tsv + # Determine enriched regions with a score threshold of 2 + python peakzilla.py -s 2 chip2.bed input2.bed > chip2_s2_peaks.tsv + # Overlap significant peaks from chip1 with enriched regions from chip2 + intersectBed -a chip1_s10_peaks.tsv -b chip2_s2_peaks.tsv > intersect_peaks.tsv + + For example datasets as well as an example of a computational pipeline for the comparative analysis of ChIP-seq datasets, please refer to our + publication: Bardet AF et al. A computational pipeline for comparative ChIP-seq analyses. Nature Protocols (2011) 7(1):45-61 (http://www.starklab.org/data/bardet_natprotoc_2011/) + + *OPTIONS* + One of peakzilla's design goals is to learn all the necessary information + from the data. The usage of the options should therefore not be required. + + *OUTPUT FORMAT* + * Results are printed as a table of tab-delimited values to stdout + * Logs are appended to logs.txt in the current directory or a custom directory/filename specified by the -l option + * Enriched regions in the control sample are written to negative_peaks.tsv or a custom directory/filename specified by the -n option + * Columns represent Chromosome / Start / End / Name / Summit / Score / ChIP / Control / FoldEnrichment / DistributionScore / FDR (%) + ]]> + + 10.1038/nprot.2011.420 + +
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