view phyloseq_plot_ordination.R @ 2:87064cb77a52 draft default tip

planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/phyloseq commit 5ec9f9e81bb9a42dec5c331dd23215ca0b027b2b
author iuc
date Sat, 16 Mar 2024 07:55:48 +0000
parents b85ba18a8f36
children
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#!/usr/bin/env Rscript

suppressPackageStartupMessages(library("optparse"))
suppressPackageStartupMessages(library("phyloseq"))

option_list <- list(
    make_option(c("--input"), action = "store", dest = "input", help = "Input file containing a phyloseq object"),
    make_option(c("--method"), action = "store", dest = "method", help = "Ordination method"),
    make_option(c("--distance"), action = "store", dest = "distance", help = "Distance method"),
    make_option(c("--type"), action = "store", dest = "type", help = "Plot type"),
    make_option(c("--output"), action = "store", dest = "output", help = "Output")
)

parser <- OptionParser(usage = "%prog [options] file", option_list = option_list)
args <- parse_args(parser, positional_arguments = TRUE)
opt <- args$options
# Construct a phyloseq object.
phyloseq_obj <- readRDS(opt$input)
# Transform data to proportions as appropriate for
# Bray-Curtis distances.
proportions_obj <- transform_sample_counts(phyloseq_obj, function(otu) otu / sum(otu))
ordination_obj <- ordinate(proportions_obj, method = opt$method, distance = opt$distance)
# Start PDF device driver and generate the plot.
dev.new()
pdf(file = opt$output)
plot_ordination(proportions_obj, ordination_obj, type = opt$type)
dev.off()