# HG changeset patch # User iuc # Date 1677961662 0 # Node ID 415cb5d91168cd979829fa96faba8edc9ff2db28 planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6 diff -r 000000000000 -r 415cb5d91168 macros.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/macros.xml Sat Mar 04 20:27:42 2023 +0000 @@ -0,0 +1,344 @@ + + + 2.5.1 + 0 + 22.01 + + + picrust2 + + + + + picrust2 + + + + +What it does +============ + +PICRUSt2 (Phylogenetic Investigation of Communities by Reconstruction of +Unobserved States) is a tool for predicting functional abundances based only on +marker gene sequences. + +Read more about the tool: https://github.com/picrust/picrust2/wiki + + + + 10.1038/s41587-020-0548-6 + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + &2 "unknown extension $_input.ext" + exit 1; +#end if +ln -s '$input' 'input.$ext' && + ]]> + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + \ No newline at end of file diff -r 000000000000 -r 415cb5d91168 metagenome_pipeline.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/metagenome_pipeline.xml Sat Mar 04 20:27:42 2023 +0000 @@ -0,0 +1,213 @@ + + to generate per-sample metagenome functional profiles based on the predicted functions for each study sequence + + macros.xml + + + + metagenome_pipeline.py -v + + + + + + + + + + + + + stratified_output['selector'] != '' and not stratified_output['wide_table'] + + + stratified_output['selector'] != '' and stratified_output['wide_table'] + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + 10.1038/s41587-020-0548-6 + + \ No newline at end of file diff -r 000000000000 -r 415cb5d91168 test-data/16S_predicted_and_nsti.tsv.gz Binary file test-data/16S_predicted_and_nsti.tsv.gz has changed diff -r 000000000000 -r 415cb5d91168 test-data/EC_predicted.tsv.gz Binary file test-data/EC_predicted.tsv.gz has changed diff -r 000000000000 -r 415cb5d91168 test-data/ec_unstrat_test.txt.gz Binary file test-data/ec_unstrat_test.txt.gz has changed diff -r 000000000000 -r 415cb5d91168 test-data/img_centroid_16S_aligned_head30.fna.gz Binary file test-data/img_centroid_16S_aligned_head30.fna.gz has changed diff -r 000000000000 -r 415cb5d91168 test-data/img_centroid_16S_aligned_head30.hmm --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/img_centroid_16S_aligned_head30.hmm Sat Mar 04 20:27:42 2023 +0000 @@ -0,0 +1,3680 @@ +HMMER3/f [3.1b2 | February 2015] +NAME img_centroid_16S_aligned_head30 +LENG 1219 +MAXL 1483 +ALPH DNA +RF no +MM no +CONS yes +CS no +MAP yes +DATE Wed Oct 10 17:19:17 2018 +NSEQ 15 +EFFN 1.680908 +CKSUM 4036506208 +STATS LOCAL MSV -13.3981 0.69577 +STATS LOCAL VITERBI -14.9113 0.69577 +STATS LOCAL FORWARD -6.1644 0.69577 +HMM A C G T + m->m m->i m->d i->m i->i d->m d->d + COMPO 1.37152 1.48531 1.23220 1.47755 + 1.26208 1.34388 1.26814 1.74440 + 0.30721 1.43238 3.65865 3.23874 0.04000 0.00000 * + 1 0.35395 2.43840 2.24483 2.25526 43 a - - - + 1.38629 1.38629 1.38629 1.38629 + 0.14543 3.65865 2.21110 1.46634 0.26236 1.09861 0.40547 + 2 2.12949 2.47053 0.36672 2.26761 44 g - - - + 1.38629 1.38629 1.38629 1.38629 + 0.05789 3.57112 3.57112 1.46634 0.26236 1.83302 0.17427 + 3 1.87475 1.35652 1.96136 0.80211 45 t - - - + 1.38629 1.38629 1.38629 1.38629 + 0.05789 3.57112 3.57112 1.46634 0.26236 1.83302 0.17427 + 4 1.83494 2.02013 0.70400 1.54583 46 g - - - + 1.38629 1.38629 1.38629 1.38629 + 0.05789 3.57112 3.57112 1.46634 0.26236 0.38546 1.13987 + 5 2.26480 2.65592 0.30114 2.45412 47 g - 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1.80636 1.16833 1234 t - - - + 1.38629 1.38629 1.38629 1.38629 + 0.08897 3.65865 2.82409 1.46634 0.26236 1.09861 0.40547 + 1189 1.17464 1.08425 1.85569 1.62674 1235 c - - - + 1.38629 1.38629 1.38629 1.38629 + 0.08830 3.62448 2.84990 1.46634 0.26236 1.45950 0.26442 + 1190 1.75151 2.29767 0.51046 2.07325 1236 g - - - + 1.38629 1.38629 1.38629 1.38629 + 0.05662 3.59280 3.59280 1.46634 0.26236 1.69992 0.20175 + 1191 1.71361 1.70728 1.01853 1.28265 1237 g - - - + 1.38629 1.38629 1.38629 1.38629 + 0.30229 3.59280 1.45520 1.46634 0.26236 0.45476 1.00677 + 1192 1.78585 1.04633 1.84861 1.12804 1238 c - - - + 1.38629 1.38629 1.38629 1.38629 + 0.06597 3.44458 3.44458 1.46634 0.26236 2.34938 0.10029 + 1193 1.53216 1.63964 1.03852 1.44434 1239 g - - - + 1.38629 1.38629 1.38629 1.38629 + 0.06597 3.44458 3.44458 1.46634 0.26236 2.34938 0.10029 + 1194 1.40755 1.31561 1.68318 1.20009 1240 t - - - + 1.57195 0.97530 1.57195 1.57195 + 0.39201 1.22969 3.44458 0.20563 1.68272 2.34938 0.10029 + 1195 1.64236 1.34649 1.30431 1.29111 1242 t - - - + 1.38629 1.38629 1.38629 1.38629 + 0.06597 3.44458 3.44458 1.46634 0.26236 2.34938 0.10029 + 1196 0.58838 2.05131 1.82480 1.86460 1243 a - - - + 1.38629 1.38629 1.38629 1.38629 + 0.06597 3.44458 3.44458 1.46634 0.26236 2.34938 0.10029 + 1197 1.13404 1.29969 1.67466 1.52197 1244 a - - - + 1.38629 1.38629 1.38629 1.38629 + 0.06597 3.44458 3.44458 1.46634 0.26236 2.34938 0.10029 + 1198 1.94221 2.21786 0.48684 2.01586 1245 g - - - + 1.38629 1.38629 1.38629 1.38629 + 0.06597 3.44458 3.44458 1.46634 0.26236 2.34938 0.10029 + 1199 1.94221 2.21786 0.48684 2.01586 1246 g - - - + 1.38629 1.38629 1.38629 1.38629 + 0.06597 3.44458 3.44458 1.46634 0.26236 2.34938 0.10029 + 1200 1.57314 1.71601 1.78160 0.81090 1247 t - - - + 1.38629 1.38629 1.38629 1.38629 + 0.06597 3.44458 3.44458 1.46634 0.26236 2.34938 0.10029 + 1201 1.59962 2.05950 0.67079 1.83756 1248 g - - - + 1.38629 1.38629 1.38629 1.38629 + 0.06597 3.44458 3.44458 1.46634 0.26236 2.34938 0.10029 + 1202 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+ 1209 1.88494 1.75948 1.92667 0.63413 1256 t - - - + 1.38629 1.38629 1.38629 1.38629 + 0.06597 3.44458 3.44458 1.46634 0.26236 2.34938 0.10029 + 1210 1.94221 2.21786 0.48684 2.01586 1257 g - - - + 1.38629 1.38629 1.38629 1.38629 + 0.06597 3.44458 3.44458 1.46634 0.26236 2.34938 0.10029 + 1211 0.58838 2.05131 1.82480 1.86460 1258 a - - - + 1.38629 1.38629 1.38629 1.38629 + 0.06597 3.44458 3.44458 1.46634 0.26236 2.34938 0.10029 + 1212 1.77113 1.21984 1.83225 0.98208 1259 t - - - + 1.38629 1.38629 1.38629 1.38629 + 0.06597 3.44458 3.44458 1.46634 0.26236 2.34938 0.10029 + 1213 1.88494 1.75948 1.92667 0.63413 1260 t - - - + 1.38629 1.38629 1.38629 1.38629 + 0.06597 3.44458 3.44458 1.46634 0.26236 2.34938 0.10029 + 1214 1.94221 2.21786 0.48684 2.01586 1261 g - - - + 1.38629 1.38629 1.38629 1.38629 + 0.06597 3.44458 3.44458 1.46634 0.26236 2.34938 0.10029 + 1215 1.94221 2.21786 0.48684 2.01586 1262 g - - - + 1.38629 1.38629 1.38629 1.38629 + 0.06597 3.44458 3.44458 1.46634 0.26236 2.34938 0.10029 + 1216 1.94221 2.21786 0.48684 2.01586 1263 g - - - + 1.38629 1.38629 1.38629 1.38629 + 0.06597 3.44458 3.44458 1.46634 0.26236 2.34938 0.10029 + 1217 1.94221 2.21786 0.48684 2.01586 1264 g - - - + 1.38629 1.38629 1.38629 1.38629 + 0.06597 3.44458 3.44458 1.46634 0.26236 2.34938 0.10029 + 1218 1.88494 1.75948 1.92667 0.63413 1265 t - - - + 1.38629 1.38629 1.38629 1.38629 + 0.06597 3.44458 3.44458 1.46634 0.26236 2.34938 0.10029 + 1219 1.94221 2.21786 0.48684 2.01586 1266 g - - - + 1.09892 1.67037 1.28581 1.59867 + 0.35693 1.20338 * 2.84032 0.06018 0.00000 * +// diff -r 000000000000 -r 415cb5d91168 test-data/img_centroid_16S_aligned_head30.model --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/img_centroid_16S_aligned_head30.model Sat Mar 04 20:27:42 2023 +0000 @@ -0,0 +1,1 @@ +GTR{1.00319/2.79077/1.5301/0.87441/3.83966/1}+FU{0.229585/0.22008/0.298596/0.251739}+G4m{0.453141}, noname = 1-1582 diff -r 000000000000 -r 415cb5d91168 test-data/img_centroid_16S_aligned_head30.tre --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/img_centroid_16S_aligned_head30.tre Sat Mar 04 20:27:42 2023 +0000 @@ -0,0 +1,1 @@ +(2511231175_test:0.00417761132822863299,(2545824660_test:0.00000100000050002909,((2519899794_test:0.01391402030675637121,2511231166_test:0.00665538545236680611):0.06273674610653526273,(2511231070_test:0.19422053808301012467,((2513237222_test:0.12148702101162445199,((2511231199_test:0.00850377323667460445,((2511231131_test:0.00170862468873008008,2519899561_test:0.00086957288189634858):0.00880326207108816233,(2511231164_test:0.00085719433669428577,2531839192_test:0.00000100000050002909):0.00346417887233879630):0.00395490298225046298):0.08865976442951280234,(2511231155_test:0.07140328680188372246,2501846300_test:0.05302326674590653044):0.00922416804481126715):0.01707145563721565798):0.06770060455925869247,2516143006_test:0.22117635610892677489):0.02999032625748145400):0.06716203994881037032):0.09307592027052699613):0.00082512996392334335,2511231196_test:0.00000100000050002909):0.0; diff -r 000000000000 -r 415cb5d91168 test-data/known_traits.tsv.gz Binary file test-data/known_traits.tsv.gz has changed diff -r 000000000000 -r 415cb5d91168 test-data/metadata.tsv --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/metadata.tsv Sat Mar 04 20:27:42 2023 +0000 @@ -0,0 +1,25 @@ +SampleID Facility Genotype +100CHE6KO PaloAlto KO +101CHE6WT PaloAlto WT +102CHE6WT PaloAlto WT +103CHE6KO PaloAlto KO +104CHE6KO PaloAlto KO +20CMK6KO Dalhousie KO +21CMK6WT Dalhousie WT +22CMK6KO Dalhousie KO +23CMK6WT Dalhousie WT +24CMK6KO Dalhousie KO +26CMK6WT Dalhousie WT +30CMK6KO Dalhousie KO +32CMK6KO Dalhousie KO +33CMK6WT Dalhousie WT +34CMK6KO Dalhousie KO +36CMK6WT Dalhousie WT +81CHE6WT PaloAlto WT +82CHE6WT PaloAlto WT +84CHE6KO PaloAlto KO +86CHE6WT PaloAlto WT +87CHE6KO PaloAlto KO +88CHE6KO PaloAlto KO +99CHE6KO PaloAlto KO +9CMK6KO Dalhousie KO diff -r 000000000000 -r 415cb5d91168 test-data/out_tree.zip Binary file test-data/out_tree.zip has changed diff -r 000000000000 -r 415cb5d91168 test-data/per_seq_func.tsv.gz Binary file test-data/per_seq_func.tsv.gz has changed diff -r 000000000000 -r 415cb5d91168 test-data/pred_metagenome_strat.tsv.gz Binary file test-data/pred_metagenome_strat.tsv.gz has changed diff -r 000000000000 -r 415cb5d91168 test-data/pred_metagenome_unstrat.tsv.gz Binary file test-data/pred_metagenome_unstrat.tsv.gz has changed diff -r 000000000000 -r 415cb5d91168 test-data/seqtab_norm.tsv.gz Binary file test-data/seqtab_norm.tsv.gz has changed diff -r 000000000000 -r 415cb5d91168 test-data/study_seqs_full.fasta --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/study_seqs_full.fasta Sat Mar 04 20:27:42 2023 +0000 @@ -0,0 +1,10 @@ +>02905cfb87861c837dde629596d9272b +TGGTCTTGACATCCCTCTGACGAGTGAGTAATGTCGCTTTCCCTTCGGGGCAGAGGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTAGTAGCCAGCAGTAAGATGGGAACTCTAGAGAGACTGCCGGGGATAACCCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCAGGGCTACACACGTGCTACAATGGCGTAAACAGAGGGAAGCGACCCTGTGAAGGTAAGCAAATCCCAAAAATAACGTCTCAGTTCGGATTGTAGTCTGCAACTCGACTACATGAAGCTGGAATCGCTAGTAATCGCGAATCAGAATGTCGCGGTGAATACGTTCCCGG +>03562a221b15ef37470e4567e112f35f +CGGGCTTGAAAGTTAGTGACCGGAGATGAAAGTCTCCTTTCTATAGCAATATAGACACGAAACTAGGTGCTGCATGGTTGTCGTCAGCTCGTGCCGTGAGGTGTCGGCTTAAGTGCCATAACGAGCGCAACCCTTTTCTTCAGTTACCATCATTAAGTTGGGGACTCTGAAGACACTGCCATCGTAAGATGTGAGGAAGGATGGGATGACGTCAAATCAGCACGGCCCTTACGTCCGGGGCGACACACGTGTTACAATGGCGTGGACAGCGGGGAACGAGGTGGCGACACCGAGGGAATCCCGAAACCACGTCCCAGTTCGGATTGGAGTCTGCAACTCGACTCCATGAAGCTGGATTCGCTAGTAATCGCGCATCAGCCATGGCGCGGTGAATACGTTC +>03af6a6644bc358ee6716cbb44a5a479 +AGGGCTTGACATATATCAGAATATACTAGAGATAGTATAGTCCTTCGGGACTGATATACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTGTCCTTAGTTGCCAGCACGTAAAGGTGGGAACTCTAAGGAGACTGCCGGTGATAAATCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCTTTATGTCCTGGGCTACACACGTACTACAATGGCCGTGACAGAGAGAAACGAAACAGTGATGTGGAGTAAAACTCTAAAAGCGGTCTCAGTTCGGATTGAAGGCTGAAATTCGCCTTCATGAAGCTGGAATTGCTAGTAATGGCAGGTCAGCATACTGCCGTGAATACGTTCCCGG +>03cb13abd3f1c5444360e489460bdfb0 +CGGGCTCAAACGGAAGGGGACGGATTGTGAAAGCAGTCTTTCCTTCGGGACCGCTTCCGAGGTGCTGCATGGTTGTCGTCAGCTCGTGCCGTGAGGTGTCGGCTTAAGTGCCATAACGAGCGCAACCCCTACCGACAGTTGCTAACAGATTAAGCTGAGGACTCTGTCGGGACTGCCGGCGCAAGCTGTGAGGAAGGCGGGGATGACGTCAAATCAGCACGGCCCTTACGTCCGGGGCGACACACGTGTTACAATGGCAGGTACAGCGGGAAGCCACCCGGCGACGGGGCGCGGAACCCGAAAACCTGTCTCAGTTCGGATTGGAGTCTGCAACCCGACTCCATGAAGCTGGATTCGCTAGTAATCGCGCATCAGCCACGGCGCGGTGAATACGTTCCCG +>05f9281835817fddd06162cd69497a2d +CGGGCTTAAATTGCATCTGAATGATTTGGAAACAGATCAGCCGCAAGGCAGATGTGAAGGTGCTGCATGGTTGTCGTCAGCTCGTGCCGTGAGGTGTCGGCTTAAGTGCCATAACGAGCGCAACCCTTGCTGTCAGTTACTAACAGGTCATGCTGAGGACTCTGACGGGACTGCCATCGTAAGATGTGAGGAAGGTGGGGATGACGTCAAATCAGCACGGCCCTTACGTCCGGGGCTACACACGTGTTACAATGGGGGGTACAGCAGGCAGCTACCTGGCGACAGGATGCTAATCCCGAAAGCCCCTCTCAGTTCGGATTGGAGTCTGCAACCCGACTCCATGAAGCTGGATTCGCTAGTAATCGCGCATCAGCCACGGCGCGGTGAATACGTTCCCGGG diff -r 000000000000 -r 415cb5d91168 test-data/study_seqs_test.fasta --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/study_seqs_test.fasta Sat Mar 04 20:27:42 2023 +0000 @@ -0,0 +1,10 @@ +>02905cfb87861c837dde629596d9272b +TGGTCTTGACATCCCTCTGACGAGTGAGTAATGTCGCTTTCCCTTCGGGGCAGAGGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTAGTAGCCAGCAGTAAGATGGGAACTCTAGAGAGACTGCCGGGGATAACCCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCAGGGCTACACACGTGCTACAATGGCGTAAACAGAGGGAAGCGACCCTGTGAAGGTAAGCAAATCCCAAAAATAACGTCTCAGTTCGGATTGTAGTCTGCAACTCGACTACATGAAGCTGGAATCGCTAGTAATCGCGAATCAGAATGTCGCGGTGAATACGTTCCCGG +>03562a221b15ef37470e4567e112f35f +CGGGCTTGAAAGTTAGTGACCGGAGATGAAAGTCTCCTTTCTATAGCAATATAGACACGAAACTAGGTGCTGCATGGTTGTCGTCAGCTCGTGCCGTGAGGTGTCGGCTTAAGTGCCATAACGAGCGCAACCCTTTTCTTCAGTTACCATCATTAAGTTGGGGACTCTGAAGACACTGCCATCGTAAGATGTGAGGAAGGATGGGATGACGTCAAATCAGCACGGCCCTTACGTCCGGGGCGACACACGTGTTACAATGGCGTGGACAGCGGGGAACGAGGTGGCGACACCGAGGGAATCCCGAAACCACGTCCCAGTTCGGATTGGAGTCTGCAACTCGACTCCATGAAGCTGGATTCGCTAGTAATCGCGCATCAGCCATGGCGCGGTGAATACGTTC +>03af6a6644bc358ee6716cbb44a5a479 +AGGGCTTGACATATATCAGAATATACTAGAGATAGTATAGTCCTTCGGGACTGATATACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTGTCCTTAGTTGCCAGCACGTAAAGGTGGGAACTCTAAGGAGACTGCCGGTGATAAATCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCTTTATGTCCTGGGCTACACACGTACTACAATGGCCGTGACAGAGAGAAACGAAACAGTGATGTGGAGTAAAACTCTAAAAGCGGTCTCAGTTCGGATTGAAGGCTGAAATTCGCCTTCATGAAGCTGGAATTGCTAGTAATGGCAGGTCAGCATACTGCCGTGAATACGTTCCCGG +>03cb13abd3f1c5444360e489460bdfb0 +CGGGCTCAAACGGAAGGGGACGGATTGTGAAAGCAGTCTTTCCTTCGGGACCGCTTCCGAGGTGCTGCATGGTTGTCGTCAGCTCGTGCCGTGAGGTGTCGGCTTAAGTGCCATAACGAGCGCAACCCCTACCGACAGTTGCTAACAGATTAAGCTGAGGACTCTGTCGGGACTGCCGGCGCAAGCTGTGAGGAAGGCGGGGATGACGTCAAATCAGCACGGCCCTTACGTCCGGGGCGACACACGTGTTACAATGGCAGGTACAGCGGGAAGCCACCCGGCGACGGGGCGCGGAACCCGAAAACCTGTCTCAGTTCGGATTGGAGTCTGCAACCCGACTCCATGAAGCTGGATTCGCTAGTAATCGCGCATCAGCCACGGCGCGGTGAATACGTTCCCG +>05f9281835817fddd06162cd69497a2d +CGGGCTTAAATTGCATCTGAATGATTTGGAAACAGATCAGCCGCAAGGCAGATGTGAAGGTGCTGCATGGTTGTCGTCAGCTCGTGCCGTGAGGTGTCGGCTTAAGTGCCATAACGAGCGCAACCCTTGCTGTCAGTTACTAACAGGTCATGCTGAGGACTCTGACGGGACTGCCATCGTAAGATGTGAGGAAGGTGGGGATGACGTCAAATCAGCACGGCCCTTACGTCCGGGGCTACACACGTGTTACAATGGGGGGTACAGCAGGCAGCTACCTGGCGACAGGATGCTAATCCCGAAAGCCCCTCTCAGTTCGGATTGGAGTCTGCAACCCGACTCCATGAAGCTGGATTCGCTAGTAATCGCGCATCAGCCACGGCGCGGTGAATACGTTCCCGGG diff -r 000000000000 -r 415cb5d91168 test-data/study_seqs_test2.fasta --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/study_seqs_test2.fasta Sat Mar 04 20:27:42 2023 +0000 @@ -0,0 +1,10 @@ +>02905cfb87861c837dde629596d9272b 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