view normalize_by_copynumber.xml @ 0:6273e2721fa3 draft

planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/picrust commit a933cd08ace9778a03e575e54f2b69bc5315e14c

<tool id="picrust_normalize_by_copy_number" name="Normalize" version="@WRAPPER_VERSION@.0">
    <description>OTUs by copy number</description>
    <macros>
        <import>macros.xml</import>
    </macros>
    <expand macro="requirements"/>
    <expand macro="version_command"/>
    <command detect_errors="aggressive"><![CDATA[
        normalize_by_copy_number.py
            -i '$input_data'
            -o tempbiom
            -c '$gg.precalc'

            @OUTPUT_CONVERSION_COMMANDS@
    ]]></command>
    <inputs>
        <param name="input_data" type="data" format="tabular,biom1" label="Input file" help="biom file or legacy QIIME OTU table"/>
        <expand macro="otu-reference-precalculated"/>
        <expand macro="biom_format_select"/>
    </inputs>
    <outputs>
        <expand macro="biom_output"/>
    </outputs>
    <tests>
        <test> <!-- test with biom input -->
            <param name="input_data" value="closed_picked_otus.biom"/>
            <param name="source" value="hist"/>
            <param name="precalc" value="16S_13_5_precalculated_minimal.tab"/>
            <param name="output_type" value="json"/>
            <output name="out_biom" ftype="json">
                <assert_contents>
                    <has_text text="Biological Observation Matrix"/>
                    <has_text text="generated_by"/>
                </assert_contents>
            </output>
        </test>
        <test> <!-- test with QIIME input and classic output -->
            <param name="input_data" value="closed_picked_otus.tab"/>
            <param name="source" value="hist"/>
            <param name="precalc" value="16S_13_5_precalculated_minimal.tab"/>
            <param name="output_type" value="tsv"/>
            <output name="out_biom" file="normalized_otus.classic" ftype="tabular"/>
        </test>
    </tests>
    <help>
<![CDATA[
@PICRUST_OVERVIEW@

**Command Documenation**

This module corrects the abundance of each OTU to better reflect the true organism abundance by normalizing by PICRUSt's prediction of 16S copy number for each OTU.

Please ensure that you have properly created your OTU table to be compatible with PICRUSt by following this guide_.
A sample file can be downloaded here_

.. _guide: http://picrust.github.com/picrust/methods/constructing_reference_otus.html
.. _here: https://raw.github.com/picrust/picrust/master/tutorials/hmp_mock_16S.tab
]]>
    </help>
    <expand macro="citations"/>
</tool>