Mercurial > repos > iuc > pycoqc
changeset 0:6fd0573c0e93 draft
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/pycoqc commit 230281ee52ab6fc5bf75bfca7cee0e9964057985"
author | iuc |
---|---|
date | Tue, 02 Mar 2021 21:09:07 +0000 |
parents | |
children | 3a2f2e24c37e |
files | macros.xml pycoqc.xml test-data/test.json test-data/test.txt |
diffstat | 4 files changed, 2388 insertions(+), 0 deletions(-) [+] |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/macros.xml Tue Mar 02 21:09:07 2021 +0000 @@ -0,0 +1,30 @@ +<?xml version="1.0"?> +<macros> + <token name="@TOOL_VERSION@">2.5.2</token> + <token name="@PROFILE@">20.01</token> + <xml name="requirements"> + <requirements> + <requirement type="package" version="@TOOL_VERSION@">pycoqc</requirement> + </requirements> + </xml> + <xml name="version_command"> + <version_command>pycoQC --version</version_command> + </xml> + <xml name="citations"> + <citations> + <citation type="bibtex">@online{, + author = {Tommaso Leonardi and Adrien Leger}, + title = {pycoQC}, + year = 2021, + url = {https://github.com/tleonardi/pycoQC}, + urldate = {2021-03-02} + }</citation> + </citations> + </xml> + <token name="@WID@"><![CDATA[ +*pycoqc* computes metrics and generates interactive QC plots for Oxford Nanopore technologies sequencing data. + ]]></token> + <token name="@REFERENCES@"><![CDATA[ +More information are available on the `website <https://tleonardi.github.io/pycoQC/>`_. + ]]></token> +</macros>
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/pycoqc.xml Tue Mar 02 21:09:07 2021 +0000 @@ -0,0 +1,97 @@ +<?xml version="1.0"?> +<tool id="pycoqc" name="Pycoqc" version="@TOOL_VERSION@+galaxy0" profile="@PROFILE@"> + <description></description> + <macros> + <import>macros.xml</import> + </macros> + <expand macro="requirements"/> + + <expand macro="version_command"/> + + <command detect_errors="exit_code"><![CDATA[ +pycoQC +--min_pass_qual '$min_pass_qual' +--min_pass_len '$min_pass_len' +#if $filter_calibration: + '$filter_calibration' +#end if +#if $filter_duplicated: + '$filter_duplicated' +#end if +--min_barcode_percent '$min_barcode_percent' +--report_title '$report_title' +## TODO in future - --template_file +## TODO in future - --config_file +#if $skip_coverage_plot + '$skip_coverage_plot' +#end if +--sample '$sample' +## TODO in future --default_config +--summary_file '$summary_file' +## TODO in future --barcode_file +#if $bam_file: + --bam_file '$bam_file' +#end if +--html_outfile '$out_html' +#if $out_json: + --json_outfile '$out_json' +#end if + ]]></command> + <inputs> + <param argument="--summary_file" type="data" format="txt,tabular" label="A sequencing_summary file" help="Must be generated by Albacore 1.0.0 + (read_fast5_basecaller.py) / Guppy 2.1.3+ (guppy_basecaller)" /> + <!-- <param argument="barcode_file" type="data" format="txt" label="" /> --> + <param argument="--bam_file" type="data" format="bam" label="An alignment file generated by aligning the reads described in the sequencing_summary file" optional="true"/> + <param name="output_json_bool" type="boolean" checked="true" label="Output JSON Summary File?"/> + <param argument="--min_pass_qual" type="integer" value="7" min="0" label="Minimum quality to consider a read as 'pass'" /> + <param argument="--min_pass_len" type="integer" value="0" min="0" label="Minimum read length to consider a read as 'pass'"/> + <param argument="--filter_calibration" type="boolean" checked="false" truevalue="--filter_calibration" falsevalue="" label="Filter Calibration?" help="If yes, reads flagged as calibration strand by the basecaller are removed" /> + <param argument="--filter_duplicated" type="boolean" checked="false" truevalue="--filter_duplicated" falsevalue="" label="Filter Duplicated?" help="If yes, duplicated read_ids are removed but the first occurence is kept (Guppy sometimes outputs the same read multiple times)" /> + <param argument="--min_barcode_percent" type="float" value="0.1" label="Minimal percent of total reads to retain barcode label" help="If below, the barcode value is set as unclassified" /> + <param argument="--report_title" type="text" value="PycoQC report" label="Title to use in the html report" /> + <param argument="--skip_coverage_plot" type="boolean" checked="false" truevalue="--skip_coverage_plot" falsevalue="" label="Skip the coverage plot in HTML report?" help="Useful when using a reference file containing many sequences, i.e. transcriptome" /> + <param argument="--sample" type="integer" label="Number of reads will be randomly selected instead of the entire dataset for ploting function" help="(deterministic sampling)" value="100000"/> + </inputs> + <outputs> + <!-- standard --> + <data name="out_html" format="html" label="${tool.name} on ${on_string}: HTML Report"/> + <data name="out_json" format="json" label="${tool.name} on ${on_string}: JSON Summary"> + <filter>output_json_bool</filter> + </data> + </outputs> + <tests> + <!-- #1 default --> + <test expect_num_outputs="2"> + <param name="summary_file" value="test.txt"/> + <output name="out_html"> + <assert_contents> + <has_line line="<!doctype html>"/> + <has_n_lines n="300"/> + </assert_contents> + </output> + <output name="out_json" file="test.json"/> + </test> + </tests> + <help><![CDATA[ +.. class:: infomark + +**What it does** + +@WID@ + +**Input** + +- Guppy Sequencing Summary Output (tsv) +- Aligned Reads (bam) (Optional) + +**Output** + +- Output QC Report (HTML) +- Output QC Metrics (JSON) + + +**References** + +@REFERENCES@ + ]]></help> + <expand macro="citations"/> +</tool>
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325 10.213778 -0.00093 80.476799 10.082108 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 6810c9bd-f867-4583-b39c-ee05a152d6f1 c4981b897c2bb47fed99916c19c9bd1bd43267a2 431 26335.119141 7.865 6292 True 26335.183594 6240 7.8 2699 11.667892 -0.001389 75.255714 10.262138 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 9094a054-3bcd-459b-9160-da810013ae9a c4981b897c2bb47fed99916c19c9bd1bd43267a2 390 26341.449219 4.15075 3320 True 26341.544922 3245 4.057 1454 10.388807 -0.001843 71.654961 9.001881 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 e3162616-3531-48da-a7a5-1fcb525ba972 c4981b897c2bb47fed99916c19c9bd1bd43267a2 61 26344.464844 2.3375 1870 True 26344.556641 1797 2.24625 674 12.127285 -0.001235 72.555145 10.442184 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 13aa4059-8bd4-4882-9fc5-b595b0f75f89 c4981b897c2bb47fed99916c19c9bd1bd43267a2 460 26349.818359 0.81275 650 True 26350.037109 474 0.59275 226 12.878458 -0.002709 77.236122 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26399.716797 1286 1.60825 557 11.564957000000001 -0.001567 76.876053 9.361953999999999 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 3612aad9-a8a9-4e07-8cb3-d4adb265e8cf c4981b897c2bb47fed99916c19c9bd1bd43267a2 330 26404.916016 16.523251000000002 13218 True 26405.003906 13148 16.435748999999998 4852 9.097014 -0.001557 77.956276 10.442177000000001 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 ac3145fa-2346-4146-9164-22368e064a5b c4981b897c2bb47fed99916c19c9bd1bd43267a2 378 26394.230469 5.49175 4393 True 26394.302734 4336 5.4205 1924 10.073883 -0.001628 78.316353 9.0919 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 aa0c6688-2a99-4383-9055-1625428c5f9b c4981b897c2bb47fed99916c19c9bd1bd43267a2 42 26348.083984 2.582 2065 True 26348.474609 1754 2.19325 718 10.079737 -0.002166 73.275299 10.082108 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 3696843a-241d-405f-83eb-c6d440a03acc c4981b897c2bb47fed99916c19c9bd1bd43267a2 282 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9.541992 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 752e8a98-9bf7-429b-b22d-5ca3977f17eb c4981b897c2bb47fed99916c19c9bd1bd43267a2 146 26338.947266 5.07725 4061 False 26338.964844 4048 5.061 1776 6.581678 -0.002549 77.236122 10.082108 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 f10c084d-b273-412b-92ff-79ff9660741e c4981b897c2bb47fed99916c19c9bd1bd43267a2 238 26389.580078 1.69475 1355 True 26389.732422 1233 1.54225 560 9.928896 -0.001771 75.795822 9.541992 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 16ec8560-0360-4bb1-ac55-47bd101a1869 c4981b897c2bb47fed99916c19c9bd1bd43267a2 512 26370.908203 1.2555 1004 True 26371.197266 772 0.9655 271 9.895582000000001 -0.002455 73.99544499999999 8.641808000000001 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 fba4159f-1ffe-4148-abf4-5715beb5bf2d c4981b897c2bb47fed99916c19c9bd1bd43267a2 457 26416.351562 0.9615 769 True 26416.398438 732 0.91525 310 10.214889 -0.002113 82.45721400000001 10.442177000000001 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 2720447a-5967-4bdf-ad48-28e542237c5b c4981b897c2bb47fed99916c19c9bd1bd43267a2 131 26403.546875 6.354 5083 False 26403.648438 5001 6.2515 2229 4.437297 -0.0016300000000000002 79.216537 9.541992 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 7d1ccba4-ab34-4dde-9a52-ba36fc7b4ca4 c4981b897c2bb47fed99916c19c9bd1bd43267a2 212 26408.207031 8.56775 6854 True 26408.570312 6564 8.20525 2648 11.378326 -0.001259 72.015038 9.902061 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 a649c902-43e2-410c-8ac2-9fcfe1061928 c4981b897c2bb47fed99916c19c9bd1bd43267a2 484 26411.943359 1.73775 1390 True 26412.119141 1250 1.56275 512 11.230636 -0.0007740000000000001 72.375107 9.902069000000001 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 68c113ee-3a18-4286-9585-21949fa57f72 c4981b897c2bb47fed99916c19c9bd1bd43267a2 453 26366.716797 8.39425 6715 True 26366.716797 6715 8.39425 3288 12.12471 -0.001567 76.155899 10.442177000000001 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 ba463cd9-c11f-451a-bc52-9b3f4a4d1e89 c4981b897c2bb47fed99916c19c9bd1bd43267a2 263 26408.056641 15.26325 12210 True 26408.136719 12147 15.1845 5016 11.314627 -0.001458 76.605995 9.81205 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 e7083085-cee3-404e-ab7e-f25f4b285ca8 c4981b897c2bb47fed99916c19c9bd1bd43267a2 236 26380.935547 0.72475 579 True 26381.001953 526 0.6585 214 10.068353 -0.0046170000000000004 76.696014 10.622223 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 fe4f55b0-504c-4078-893a-ad4f80a228ef c4981b897c2bb47fed99916c19c9bd1bd43267a2 154 26352.123047 0.83625 669 True 26352.162109 638 0.7975 289 9.956733999999999 -0.002187 78.856461 9.361953999999999 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 480699bf-4aed-4aea-a7eb-d0bc01b53ef0 c4981b897c2bb47fed99916c19c9bd1bd43267a2 131 26390.242188 8.3315 6665 False 26390.373047 6559 8.199 2578 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12.462242999999999 -0.000951 74.895638 9.722031 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 10dbed68-a818-413a-9dce-52ce2da54e09 c4981b897c2bb47fed99916c19c9bd1bd43267a2 394 26310.148438 62.397251000000004 49917 True 26310.384766 49728 62.160999 18831 7.484535 -0.001398 78.856461 9.361953999999999 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 19ce46e3-ae63-4849-9772-9fdfdd7bd7f8 c4981b897c2bb47fed99916c19c9bd1bd43267a2 308 26384.623047 15.58825 12470 True 26384.710938 12402 15.50325 4938 10.037623 -0.001557 81.55703000000001 10.0821 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 20786338-1b33-451b-ba7f-cba110be3649 c4981b897c2bb47fed99916c19c9bd1bd43267a2 333 26371.640625 2.1775 1742 True 26371.796875 1617 2.02125 771 11.898175 -0.0020570000000000002 78.316353 9.722031 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 b2a0b3e6-cd78-4130-a12b-be7289facac8 c4981b897c2bb47fed99916c19c9bd1bd43267a2 428 26343.695312 0.8445 675 True 26343.816406 579 0.7245 201 8.576042999999999 -0.004 73.095261 10.982292 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 5956702e-ce3e-4e7a-83d4-dfac9c1c3b90 c4981b897c2bb47fed99916c19c9bd1bd43267a2 240 26383.267578 1.64225 1313 True 26383.357422 1241 1.55225 559 14.650373000000002 -0.001745 76.696014 9.541992 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 dc6c7b8f-40fa-4905-bb10-1b084ef8ca3c c4981b897c2bb47fed99916c19c9bd1bd43267a2 160 26398.804688 5.14 4112 True 26398.939453 4002 5.0025 1732 12.989658 -0.001583 75.075676 10.262146000000001 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 3df58999-de48-41b3-a654-fac38409ad6e c4981b897c2bb47fed99916c19c9bd1bd43267a2 327 26407.894531 2.923 2338 True 26408.054688 2210 2.763 1041 11.915230000000001 -0.0022600000000000003 77.776237 10.262146000000001 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 31bfcdf8-6f0b-4d89-bb7f-5ac30416e3d2 c4981b897c2bb47fed99916c19c9bd1bd43267a2 435 26356.859375 7.90375 6323 True 26357.011719 6202 7.7525 2950 12.810169 -0.001245 79.936691 9.902069000000001 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 1ea77e5d-0e07-4576-bcab-509a96a5e945 c4981b897c2bb47fed99916c19c9bd1bd43267a2 82 26343.238281 1.375 1100 True 26343.464844 920 1.15 441 11.922711 -0.001715 75.975861 10.622223 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 99c00e3c-4ea3-4dd0-94fb-9f509654f871 c4981b897c2bb47fed99916c19c9bd1bd43267a2 351 26403.734375 10.21125 8169 True 26403.787109 8126 10.1575 3668 11.732319 -0.00126 74.895638 9.722031 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 2df0eac2-6298-4452-b98b-b04291417928 c4981b897c2bb47fed99916c19c9bd1bd43267a2 369 26317.042969 35.474499 28379 True 26317.087891 28342 35.428249 12798 12.20998 -0.001492 73.99544499999999 10.0821 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 e02aabb2-f952-4894-b8ab-9f707fd4a03c c4981b897c2bb47fed99916c19c9bd1bd43267a2 24 26364.498047 2.0925 1674 True 26364.732422 1486 1.8575 727 10.702371000000001 -0.001732 77.956276 9.001877 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 9219d9dd-b432-4710-9c7d-5def90e50c49 c4981b897c2bb47fed99916c19c9bd1bd43267a2 25 26352.966797 1.18175 945 True 26353.089844 846 1.058 385 11.540395 -0.0007700000000000001 81.55703000000001 9.361953999999999 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 63ae3002-e9b0-4e18-9dea-8997d0265af4 c4981b897c2bb47fed99916c19c9bd1bd43267a2 297 26419.556641 1.88375 1507 True 26419.628906 1449 1.81125 397 7.273964 -0.0034289999999999998 76.155899 9.541992 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 f890de94-7a92-4dfe-afb4-f21804a8dfd0 c4981b897c2bb47fed99916c19c9bd1bd43267a2 88 26356.021484 12.2725 9818 True 26356.048828 9796 12.245 4872 10.148813 -0.001531 78.316353 9.181915 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 dc776f5a-1556-4134-ada8-d682891870d8 c4981b897c2bb47fed99916c19c9bd1bd43267a2 71 26398.970703 9.454 7563 True 26399.349609 7259 9.074 3192 9.798524 -0.001936 79.396576 8.101692 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 85428441-c8c2-4258-9a88-145e913f98d9 c4981b897c2bb47fed99916c19c9bd1bd43267a2 124 26367.28125 6.94575 5556 True 26367.583984 5315 6.6445 2869 11.671432000000001 -0.001501 75.075676 9.902069000000001 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 70d8e249-4d86-4174-8e0e-4f3ffb837d08 c4981b897c2bb47fed99916c19c9bd1bd43267a2 41 26357.1875 4.45925 3567 True 26357.556641 3273 4.09175 2074 10.364952 -0.001452 80.296761 10.262138 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 85f67218-9784-46a9-ad34-4660dd8e93cd c4981b897c2bb47fed99916c19c9bd1bd43267a2 188 26344.775391 1.8585 1486 True 26345.232422 1122 1.4035 437 10.76446 -0.002787 73.63537600000001 9.992085000000001 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 6f45e62a-c70d-44ec-aba7-30a927f83943 c4981b897c2bb47fed99916c19c9bd1bd43267a2 186 26361.884766 6.058 4846 True 26361.990234 4762 5.953 1944 10.502931 -0.001791 75.435753 9.902061 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 cd8c437f-167e-48ac-ba77-5bffc54c34e9 c4981b897c2bb47fed99916c19c9bd1bd43267a2 308 26372.695312 1.43825 1150 True 26372.853516 1025 1.282 466 9.058945 -0.002388 83.357407 10.262138 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 931f48db-791b-48e4-96af-cfcfc846fd3e c4981b897c2bb47fed99916c19c9bd1bd43267a2 448 26374.933594 1.51075 1208 True 26375.076172 1095 1.3695 506 13.558441 -0.002861 79.486595 9.451972999999999 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 93355adf-8e71-474e-a646-99d20cdc0d0a c4981b897c2bb47fed99916c19c9bd1bd43267a2 178 26343.005859 11.03025 8824 True 26343.005859 8824 11.03025 4393 11.332175 -0.001362 76.155899 10.082108 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 08f6278c-297b-45ba-bc79-c1848e2f4646 c4981b897c2bb47fed99916c19c9bd1bd43267a2 244 26380.472656 6.9335 5546 True 26380.505859 5519 6.89975 2654 11.171 -0.001399 77.776237 9.541992 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 9cd938ed-98e2-47f8-9c7e-2e5b57546eb9 c4981b897c2bb47fed99916c19c9bd1bd43267a2 263 26323.029297 20.21575 16172 True 26323.136719 16087 20.109501 6852 12.671194999999999 -0.001256 75.61578399999999 10.442177000000001 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 93790dc8-cade-426b-9f66-189d6d425e64 c4981b897c2bb47fed99916c19c9bd1bd43267a2 87 26372.099609 0.9615 769 True 26372.203125 687 0.859 256 11.069284 -0.00262 71.11484499999999 10.082108 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 31b79f8f-a766-4cc8-81e3-01dbf43375b3 c4981b897c2bb47fed99916c19c9bd1bd43267a2 489 26422.992188 1.77225 1417 True 26423.117188 1317 1.64725 606 12.527446000000001 -0.0018510000000000002 77.956276 9.541992 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 fa1c873f-5978-41a9-af01-f181314eff73 c4981b897c2bb47fed99916c19c9bd1bd43267a2 446 26388.439453 3.85575 3084 True 26388.632812 2931 3.6645 1367 12.720956 -0.001532 75.435753 9.541992 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 c8f39065-f9f2-4493-80bb-1af1d3a0a4f8 c4981b897c2bb47fed99916c19c9bd1bd43267a2 204 26390.773438 5.59775 4478 True 26390.865234 4405 5.5065 2144 10.954533 -0.001786 81.55703000000001 8.461769 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 df9384e4-1aaa-4f8e-91ed-50f78b3e0353 c4981b897c2bb47fed99916c19c9bd1bd43267a2 13 26390.564453 33.407749 26726 True 26390.626953 26677 33.3465 12377 8.624769 -0.001609 78.496391 10.262138 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 23b027ed-4471-4ddd-a89e-e23720c99e62 c4981b897c2bb47fed99916c19c9bd1bd43267a2 463 26352.242188 2.82725 2261 False 26352.390625 2142 2.6785 928 6.021628 -0.0023350000000000003 67.334061 9.541992 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 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74.535561 9.361953999999999 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 9f7f7c60-16c9-49bd-bf22-d97989c9e6d0 c4981b897c2bb47fed99916c19c9bd1bd43267a2 332 26409.148438 1.90775 1526 True 26409.185547 1496 1.87025 599 11.275158 -0.0014939999999999999 79.936691 9.541992 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 233b5fe4-430a-41b4-8ade-258d1b5fbb04 c4981b897c2bb47fed99916c19c9bd1bd43267a2 424 26411.376953 2.86675 2293 True 26411.556641 2150 2.688 969 11.677593 -0.001534 77.956276 9.541992 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 1d7aa252-a0ec-4ed2-99f5-fc295dd948c3 c4981b897c2bb47fed99916c19c9bd1bd43267a2 185 26392.21875 2.46675 1973 True 26392.234375 1961 2.45175 897 10.161137 -0.00197 76.696014 9.001884 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 e5737850-ee1f-4f0c-b399-a2292f45ee2e c4981b897c2bb47fed99916c19c9bd1bd43267a2 389 26329.912109 17.613001 14090 True 26329.943359 14064 17.5805 7075 12.144423 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-0.002165 70.034622 9.902069000000001 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 d04ebf22-c08e-4c15-b13e-37191370435e c4981b897c2bb47fed99916c19c9bd1bd43267a2 427 26341.130859 0.898 718 True 26341.173828 683 0.85425 382 8.151000999999999 -0.0030269999999999997 80.296761 8.821838 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 9c03e77e-cb0c-449d-9392-246fea5b521b c4981b897c2bb47fed99916c19c9bd1bd43267a2 266 26321.982422 76.537498 61230 True 26321.982422 61230 76.537498 27423 12.608644 -0.001408 75.795822 10.262146000000001 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 a1823e8a-acef-4e14-acd2-1772312326e1 c4981b897c2bb47fed99916c19c9bd1bd43267a2 503 26364.527344 4.6375 3710 True 26364.691406 3579 4.47375 1946 12.57206 -0.001126 70.574738 10.082108 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 d4fc1082-86cb-4f2c-bcb8-51d7d5c9ce2f c4981b897c2bb47fed99916c19c9bd1bd43267a2 118 26407.423828 5.3325 4266 True 26407.597656 4126 5.1575 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75.975861 8.821846 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 948bd431-3a15-42c2-9447-b3466c138d0e c4981b897c2bb47fed99916c19c9bd1bd43267a2 116 26405.417969 18.70225 14961 True 26405.496094 14899 18.62475 6811 11.580996 -0.00121 71.47492199999999 9.361953999999999 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 3dc28069-22e3-4f7c-bc10-74373f132f3e c4981b897c2bb47fed99916c19c9bd1bd43267a2 54 26393.277344 2.466 1972 True 26393.460938 1826 2.2835 923 9.710075999999999 -0.001757 70.21466099999999 9.541992 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 f415b0de-9c3c-44ba-9bcd-f51f58f054fa c4981b897c2bb47fed99916c19c9bd1bd43267a2 143 26397.763672 2.27375 1819 True 26398.007812 1624 2.03 735 11.780375 -0.001797 78.496391 9.722023 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 c325677c-2618-4691-850d-f4e448d9be69 c4981b897c2bb47fed99916c19c9bd1bd43267a2 319 26378.070312 4.15675 3325 True 26378.130859 3276 4.0955 1467 11.022216 -0.0018210000000000001 73.095261 9.541992 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 1fdb56c2-878c-484d-978f-77ef41b3c8ce c4981b897c2bb47fed99916c19c9bd1bd43267a2 410 26386.667969 6.9855 5588 True 26387.574219 4864 6.0805 2105 11.305429 -0.001507 83.897514 10.442177000000001 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 5801e27d-3ddd-486e-85cb-9d7762191804 c4981b897c2bb47fed99916c19c9bd1bd43267a2 129 26405.677734 1.128 902 True 26405.677734 902 1.128 349 8.195438000000001 -0.001983 70.93480699999999 9.181915 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 b8b9ba4c-dacb-48c4-b6cd-1aebd3d835e4 c4981b897c2bb47fed99916c19c9bd1bd43267a2 493 26379.412109 11.1655 8932 True 26379.427734 8918 11.148 3806 11.47044 -0.001718 77.596199 9.541992 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 a290e95b-cd7b-45d0-a333-2ed9f3782d2e c4981b897c2bb47fed99916c19c9bd1bd43267a2 378 26385.9375 5.609 4487 True 26386.033203 4410 5.51275 1955 10.562033999999999 -0.001385 77.596199 9.902069000000001 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 183036a2-eb22-4f7e-b0e3-1e860b0796fb c4981b897c2bb47fed99916c19c9bd1bd43267a2 448 26336.53125 37.89925 30319 True 26336.667969 30211 37.764252 12839 13.260437 -0.001276 78.136314 10.082108 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 4f2793db-b88c-4127-9958-31af7179f0c0 c4981b897c2bb47fed99916c19c9bd1bd43267a2 267 26369.166016 3.316 2652 True 26369.238281 2594 3.2435 1006 11.318565 -0.001562 77.05609100000001 9.722023 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 2dba62d3-e20a-4b17-b90d-debc251c1139 c4981b897c2bb47fed99916c19c9bd1bd43267a2 416 26404.839844 1.01875 815 True 26405.009766 679 0.84875 260 12.141273 -0.001802 76.876053 10.262138 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 ff046b69-f90f-46fd-9630-aeac9780d8f7 c4981b897c2bb47fed99916c19c9bd1bd43267a2 128 26368.283203 7.026 5620 True 26368.507812 5440 6.801 2452 12.195428999999999 -0.001702 67.87416800000001 9.361953999999999 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 1cddc367-24d6-4ef3-bf2c-cba8d4c17afb c4981b897c2bb47fed99916c19c9bd1bd43267a2 398 26358.044922 1.539 1231 True 26358.119141 1169 1.4615 517 10.923638 -0.002424 76.155899 9.902069000000001 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 d2f89a68-97cb-41e7-a5c6-e2805c258159 c4981b897c2bb47fed99916c19c9bd1bd43267a2 380 26359.154297 8.39075 6712 True 26359.199219 6676 8.34575 3103 8.84262 -0.002195 77.05609100000001 9.722031 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 0b9a6fb1-2cb9-4d75-9ac1-1d1c5badf063 c4981b897c2bb47fed99916c19c9bd1bd43267a2 470 26414.964844 8.0335 6426 True 26415.058594 6351 7.93975 2719 12.785998 -0.001604 75.075676 10.442184 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 7df4e095-dbe5-48ce-b2c1-752807e2daff c4981b897c2bb47fed99916c19c9bd1bd43267a2 88 26415.6875 2.58275 2066 True 26415.888672 1907 2.384 791 11.832934 -0.003054 78.316353 11.162331 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 9759c253-f3c8-40d9-9495-1f23fbc6279e c4981b897c2bb47fed99916c19c9bd1bd43267a2 129 26342.785156 2.86575 2292 True 26343.052734 2080 2.60075 920 10.239228 -0.0021260000000000003 73.095261 10.442177000000001 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 6458f8da-42d7-415b-922f-ee306e93c7b4 c4981b897c2bb47fed99916c19c9bd1bd43267a2 306 26380.085938 1.146 916 True 26380.150391 865 1.08225 376 11.436308 -0.0018789999999999998 77.596199 10.262146000000001 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 86588264-cc1d-43fe-9e54-7ef01b84a09f c4981b897c2bb47fed99916c19c9bd1bd43267a2 164 26337.714844 5.81425 4651 True 26337.748047 4624 5.7805 2165 11.806991 -0.001491 77.416161 10.622223 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 9768c947-2c72-4afb-837b-69e103d2f6d5 c4981b897c2bb47fed99916c19c9bd1bd43267a2 398 26378.871094 14.42225 11537 True 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26359.613281 45030 56.28775 18615 10.168434 -0.0015689999999999999 77.956276 9.902069000000001 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 a7e37c49-9c40-41bc-8a54-f713974ba686 c4981b897c2bb47fed99916c19c9bd1bd43267a2 19 26333.65625 19.2705 15416 True 26333.820312 15284 19.105499 6664 12.278858999999999 -0.00144 77.956276 10.262138 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 f2fc3aed-9f5c-4c86-82e5-fb881d1588df c4981b897c2bb47fed99916c19c9bd1bd43267a2 251 26421.279297 10.40075 8320 True 26421.302734 8302 10.37825 3843 11.293560000000001 -0.0016460000000000001 76.696014 9.902069000000001 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 dfbb7507-d2ed-4159-a018-fa3ae0dee825 c4981b897c2bb47fed99916c19c9bd1bd43267a2 357 26367.285156 2.431 1944 True 26367.349609 1893 2.36725 932 9.773023 -0.0015300000000000001 76.155899 10.982292 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 cc9430cb-1d7f-48a6-971d-a7e80fb615da 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26384.460938 992 1.241 475 8.521074 -0.0008849999999999999 78.316353 9.001884 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 36223c79-4d36-40df-9922-909a101203e0 c4981b897c2bb47fed99916c19c9bd1bd43267a2 366 26396.484375 2.08475 1667 False 26396.632812 1548 1.936 635 4.115908999999999 -0.0022600000000000003 66.973984 9.181919 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 4ec50ed2-93d9-4d62-bbb1-23b02f20fb59 c4981b897c2bb47fed99916c19c9bd1bd43267a2 199 26398.1875 3.50525 2804 True 26398.234375 2768 3.46025 1159 12.033625 -0.001538 77.956276 9.722031 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 3a518460-29e6-4a61-ae13-311cc98d5c91 c4981b897c2bb47fed99916c19c9bd1bd43267a2 253 26391.978516 26.1905 20952 True 26392.357422 20648 25.810499 9233 12.867681 -0.001259 72.375107 9.722027 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 0a0606f0-af7c-4ed1-968c-3952e67cb0fc c4981b897c2bb47fed99916c19c9bd1bd43267a2 56 26342.916016 7.514 6011 True 26343.015625 5931 7.414 2355 11.338392 -0.001789 76.696014 9.541992 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 de36b491-b401-4377-ad88-f14f7d983365 c4981b897c2bb47fed99916c19c9bd1bd43267a2 99 26419.455078 1.088 870 True 26419.509766 827 1.03425 480 12.818291 -0.00281 67.87416800000001 9.361953999999999 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 92f63776-e893-4f2a-97b4-52661cd9f60e c4981b897c2bb47fed99916c19c9bd1bd43267a2 285 26362.947266 5.73025 4584 False 26362.982422 4557 5.6965 1672 4.659231 -0.001584 53.291126 9.361953999999999 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 4516388c-b9c6-4a5d-82e3-9dcf3e6b3dda c4981b897c2bb47fed99916c19c9bd1bd43267a2 375 26420.582031 2.78175 2225 True 26420.664062 2160 2.7005 982 12.639355 -0.001577 75.435753 9.902069000000001 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 31a7965b-2cff-458b-994a-c72e54804c25 c4981b897c2bb47fed99916c19c9bd1bd43267a2 471 26423.171875 1.8275 1462 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26410.806641 1389 1.73625 619 13.085728999999999 -0.00125 77.596199 9.541992 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 db575289-ef16-4e8d-a326-d06c54a9e3fd c4981b897c2bb47fed99916c19c9bd1bd43267a2 27 26376.816406 3.57 2856 True 26376.873047 2809 3.51125 1137 10.780906 -0.001271 77.416161 10.802254 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 dabf5638-561d-4ff5-9810-9d4eef46327b c4981b897c2bb47fed99916c19c9bd1bd43267a2 85 26400.21875 1.049 839 True 26400.232422 827 1.034 363 11.93522 -0.002003 75.075676 9.361953999999999 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 5edfabbe-9a60-41fc-a2fd-faba254f68db c4981b897c2bb47fed99916c19c9bd1bd43267a2 293 26340.855469 1.789 1431 True 26341.005859 1311 1.639 637 12.032103 -0.00256 71.47492199999999 8.281731 +FAK29099_c4981b897c2bb47fed99916c19c9bd1bd43267a2_317.fast5 77b2bfc8-b6d7-4674-91b4-9157de9eb7f2 c4981b897c2bb47fed99916c19c9bd1bd43267a2 364 26392.556641 37.18325 29746 True 26393.083984 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