Mercurial > repos > iuc > raceid_clustering
comparison scripts/trajectoryinspect.R @ 10:49776718ae90 draft default tip
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/raceid3 commit 0ffa71ef9f8d020fe7ba94502db8cec26fd8741f
author | iuc |
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date | Tue, 05 Nov 2024 16:33:40 +0000 |
parents | 0bff0ee0683a |
children |
comparison
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9:0bff0ee0683a | 10:49776718ae90 |
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2 VERSION <- "0.2" # nolint | 2 VERSION <- "0.2" # nolint |
3 | 3 |
4 args <- commandArgs(trailingOnly = TRUE) | 4 args <- commandArgs(trailingOnly = TRUE) |
5 | 5 |
6 if (length(args) != 1) { | 6 if (length(args) != 1) { |
7 message(paste("VERSION:", VERSION)) | 7 message(paste("VERSION:", VERSION)) |
8 stop("Please provide the config file") | 8 stop("Please provide the config file") |
9 } | 9 } |
10 | 10 |
11 suppressWarnings(suppressPackageStartupMessages(require(RaceID))) | 11 suppressWarnings(suppressPackageStartupMessages(require(RaceID))) |
12 suppressWarnings(suppressPackageStartupMessages(require(FateID))) | 12 suppressWarnings(suppressPackageStartupMessages(require(FateID))) |
13 source(args[1]) | 13 source(args[1]) |
32 ltr, | 32 ltr, |
33 do.call("makeBranchLink", as.list(trjsid.branchcells.ijk)) | 33 do.call("makeBranchLink", as.list(trjsid.branchcells.ijk)) |
34 ) | 34 ) |
35 write.table( | 35 write.table( |
36 head(bra$diffgenes$z, trjsid.numdiffgenes), | 36 head(bra$diffgenes$z, trjsid.numdiffgenes), |
37 file = out.diffgenes) | 37 file = out.diffgenes |
38 ) | |
38 | 39 |
39 par(mfrow = c(3, 2), cex = 0.5) | 40 par(mfrow = c(3, 2), cex = 0.5) |
40 print(do.call(plotmap, c(bra$scl, final = FALSE, fr = FALSE))) | 41 print(do.call(plotmap, c(bra$scl, final = FALSE, fr = FALSE))) |
41 print(do.call(mtext, c("Initial Clusters (tSNE)", test))) | 42 print(do.call(mtext, c("Initial Clusters (tSNE)", test))) |
42 print(do.call(plotmap, c(bra$scl, final = TRUE, fr = FALSE))) | 43 print(do.call(plotmap, c(bra$scl, final = TRUE, fr = FALSE))) |
61 trjfid_getsom$x <- fs | 62 trjfid_getsom$x <- fs |
62 s1d <- do.call(getsom, c(trjfid_getsom)) | 63 s1d <- do.call(getsom, c(trjfid_getsom)) |
63 trjfid_procsom$s1d <- s1d | 64 trjfid_procsom$s1d <- s1d |
64 ps <- do.call(procsom, c(trjfid_procsom)) | 65 ps <- do.call(procsom, c(trjfid_procsom)) |
65 | 66 |
66 y <- ltr@sc@cpart[n$f] | 67 y <- ltr@sc@cpart[n$f] |
67 fcol <- ltr@sc@fcol | 68 fcol <- ltr@sc@fcol |
68 | 69 |
69 trjfid_plotheat$xpart <- y | 70 trjfid_plotheat$xpart <- y |
70 trjfid_plotheat$xcol <- fcol | 71 trjfid_plotheat$xcol <- fcol |
71 | 72 |
72 test$side <- 3 | 73 test$side <- 3 |
73 test$line <- 3 | 74 test$line <- 3 |
74 | 75 |
75 ##Plot average z-score for all modules derived from the SOM: | 76 ## Plot average z-score for all modules derived from the SOM: |
76 trjfid_plotheat$x <- ps$nodes.z | 77 trjfid_plotheat$x <- ps$nodes.z |
77 trjfid_plotheat$ypart <- unique(ps$nodes) | 78 trjfid_plotheat$ypart <- unique(ps$nodes) |
78 print(do.call(plotheatmap, c(trjfid_plotheat))) | 79 print(do.call(plotheatmap, c(trjfid_plotheat))) |
79 print(do.call(mtext, c("Average z-score for all modules derived from SOM", | 80 print(do.call(mtext, c( |
80 test))) | 81 "Average z-score for all modules derived from SOM", |
81 ##Plot z-score profile of each gene ordered by SOM modules: | 82 test |
83 ))) | |
84 ## Plot z-score profile of each gene ordered by SOM modules: | |
82 trjfid_plotheat$x <- ps$all.z | 85 trjfid_plotheat$x <- ps$all.z |
83 trjfid_plotheat$ypart <- ps$nodes | 86 trjfid_plotheat$ypart <- ps$nodes |
84 print(do.call(plotheatmap, c(trjfid_plotheat))) | 87 print(do.call(plotheatmap, c(trjfid_plotheat))) |
85 print(do.call(mtext, c(paste0("z-score profile of each gene", | 88 print(do.call(mtext, c(paste0( |
86 "ordered by SOM modules"), test))) | 89 "z-score profile of each gene", |
87 ##Plot normalized expression profile of each gene ordered by SOM modules: | 90 "ordered by SOM modules" |
91 ), test))) | |
92 ## Plot normalized expression profile of each gene ordered by SOM modules: | |
88 trjfid_plotheat$x <- ps$all.e | 93 trjfid_plotheat$x <- ps$all.e |
89 trjfid_plotheat$ypart <- ps$nodes | 94 trjfid_plotheat$ypart <- ps$nodes |
90 print(do.call(plotheatmap, c(trjfid_plotheat))) | 95 print(do.call(plotheatmap, c(trjfid_plotheat))) |
91 print(do.call(mtext, c(paste0("Normalized expression profile of each", | 96 print(do.call(mtext, c(paste0( |
92 "gene ordered by SOM modules"), test))) | 97 "Normalized expression profile of each", |
93 ##Plot binarized expression profile of each gene | 98 "gene ordered by SOM modules" |
94 ##(z-score < -1, -1 < z-score < 1, z-score > 1) | 99 ), test))) |
100 ## Plot binarized expression profile of each gene | |
101 ## (z-score < -1, -1 < z-score < 1, z-score > 1) | |
95 trjfid_plotheat$x <- ps$all.b | 102 trjfid_plotheat$x <- ps$all.b |
96 trjfid_plotheat$ypart <- ps$nodes | 103 trjfid_plotheat$ypart <- ps$nodes |
97 print(do.call(plotheatmap, c(trjfid_plotheat))) | 104 print(do.call(plotheatmap, c(trjfid_plotheat))) |
98 print(do.call(mtext, c("Binarized expression profile of each gene", test))) | 105 print(do.call(mtext, c("Binarized expression profile of each gene", test))) |
99 ## This should be written out, and passed back into the tool | 106 ## This should be written out, and passed back into the tool |