Mercurial > repos > iuc > raceid_inspecttrajectory
diff raceid_inspecttrajectories.xml @ 0:e0e9b24d76aa draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/raceid3 commit f880060c478d42202df5b78a81329f8af56b1138
author | iuc |
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date | Thu, 22 Nov 2018 04:42:18 -0500 |
parents | |
children | 86e2358cf273 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/raceid_inspecttrajectories.xml Thu Nov 22 04:42:18 2018 -0500 @@ -0,0 +1,57 @@ +<tool id="raceid_inspecttrajectory" name="Lineage Branch Analysis using StemID" version="@VERSION_RACEID@.@VERSION_PACKAGE@.1" > + <description>inspects branches of a lineage tree</description> + <macros> + <import>macros.xml</import> + <import>macros_inspecttrajectories.xml</import> + </macros> + <expand macro="requirements" /> + <version_command><![CDATA[ +Rscript '$__tool_directory__/scripts/trajectoryinspect.R' @GET_VERSION@ +]]></version_command> + + <command detect_errors="exit_code"><![CDATA[ +#set bin = 'trajectoryinspect.R' +Rscript '$__tool_directory__/scripts/$bin' '$userconf' 2>&1 > '$outlog' + ]]></command> + + <configfiles> + <configfile name="userconf" ><![CDATA[ +@STRING2VECTOR@ + +@INSPECTTRAJECTORIES_CHEETAH@ +]]> + </configfile> + </configfiles> + + <inputs> + <expand macro="inspecttrajectories_inputs" /> + </inputs> + + <outputs> + <data name="outpdf" format="pdf" label="${tool.name} on ${on_string}: PDF Report" /> + <data name="outdiffgenes" format="tabular" label="${tool.name} on ${on_string}: TrajectoryInspect - Differential Genes" > + <filter>trjsid['basic']['doit'] == "yes"</filter> + </data> + <data name="outlog" format="txt" label="${tool.name} on ${on_string}: Log" > + <filter>use_log</filter> + </data> + + </outputs> + <tests> + <expand macro="inspecttrajectories_tests" /> + </tests> + <help><![CDATA[ +StemID2 and FateID +===================== + +Given a previous lineage tree generated an RDS file generated from the previous Trajectory step, we can explore the variation of gene expression for all cells that lie on a given branch or trajectory. + +This will generate a PDF containing a heatmap of expression for all neighboring clusters that share links with cluster 5, as well as a plot of all cells along the branches between 1 to 3 and 3 to 10. A table of the most differentially expressed genes across these projection will also be output, which will provide a more qualitative assessment of how signficant our Apoa-expressing genes are along this projection. + +For more information on the different types cluster and trajectory inspection that can be performed, please consult the RaceID vignette_. + +.. _vignette: https://github.com/dgrun/RaceID3_StemID2_package/blob/master/vignettes/RaceID.Rmd + +]]></help> + <expand macro="citations" /> +</tool>