Mercurial > repos > iuc > rgrnastar
comparison macros.xml @ 10:d82339e37e8e draft
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/rgrnastar commit 686574b0392e554b75035a9b79bc919dfda9ab97"
author | iuc |
---|---|
date | Thu, 15 Aug 2019 01:54:13 -0400 |
parents | d5659efd66aa |
children | 41929aa1e7f3 |
comparison
equal
deleted
inserted
replaced
9:0a563fd2f22e | 10:d82339e37e8e |
---|---|
1 <macros> | 1 <macros> |
2 <!-- REMEMBER to bump the version of rna_star_index_builder_data_manager | |
3 whenever you make changes to the following two version tokens! | |
4 The data manager uses a symlink to this macro file to keep the versions in | |
5 sync. --> | |
6 <!-- STAR version to be used --> | |
7 <token name="@VERSION@">2.7.2a</token> | |
8 <!-- STAR index version compatible with this version of STAR | |
9 This is the STAR version that introduced the index structure expected | |
10 by the current version. | |
11 It can be found for any specific version of STAR with: | |
12 STAR -h | grep versionGenome | |
13 or by looking for the versionGenome parameter in source/parametersDefault | |
14 of STAR's source code --> | |
15 <token name="@IDX_VERSION@">2.7.1a</token> | |
16 | |
2 <xml name="requirements"> | 17 <xml name="requirements"> |
3 <requirements> | 18 <requirements> |
4 <requirement type="package" version="2.6.0b">star</requirement> | 19 <requirement type="package" version="@VERSION@">star</requirement> |
5 <requirement type="package" version="1.8">samtools</requirement> | 20 <requirement type="package" version="1.9">samtools</requirement> |
6 </requirements> | 21 </requirements> |
7 </xml> | 22 </xml> |
23 | |
24 <xml name="index_selection" token_with_gene_model="1"> | |
25 <param argument="--genomeDir" name="genomeDir" type="select" | |
26 label="Select reference genome" | |
27 help="If your genome of interest is not listed, contact the Galaxy team"> | |
28 <options from_data_table="rnastar_index2_versioned"> | |
29 <filter type="static_value" column="4" value="@WITH_GENE_MODEL@" /> | |
30 <filter type="static_value" column="5" value="@IDX_VERSION@" /> | |
31 <filter type="sort_by" column="2" /> | |
32 <validator type="no_options" message="No indexes are available for the selected input dataset" /> | |
33 </options> | |
34 </param> | |
35 </xml> | |
36 | |
8 <token name="@FASTQ_GZ_OPTION@"> | 37 <token name="@FASTQ_GZ_OPTION@"> |
9 --readFilesCommand zcat | 38 --readFilesCommand zcat |
10 </token> | 39 </token> |
11 <xml name="citations"> | 40 <xml name="citations"> |
12 <citations> | 41 <citations> |
13 <citation type="doi">10.1093/bioinformatics/bts635</citation> | 42 <citation type="doi">10.1093/bioinformatics/bts635</citation> |
14 </citations> | 43 </citations> |
15 </xml> | 44 </xml> |
16 <xml name="@SJDBOPTIONS@"> | 45 <xml name="@SJDBOPTIONS@" token_optional="true"> |
17 <param argument="--sjdbGTFfile" type="data" format="gff3,gtf" label="Gene model (gff3,gtf) file for splice junctions" optional="true" help="Exon junction information for mapping splices"/> | 46 <param argument="--sjdbGTFfile" type="data" format="gff3,gtf" label="Gene model (gff3,gtf) file for splice junctions" optional="@OPTIONAL@" help="Exon junction information for mapping splices"/> |
18 <param argument="--sjdbOverhang" type="integer" min="1" value="100" label="Length of the genomic sequence around annotated junctions" help="Used in constructing the splice junctions database. Ideal value is ReadLength-1"/> | 47 <param argument="--sjdbOverhang" type="integer" min="1" value="100" label="Length of the genomic sequence around annotated junctions" help="Used in constructing the splice junctions database. Ideal value is ReadLength-1"/> |
19 </xml> | 48 </xml> |
20 <xml name="dbKeyActions"> | 49 <xml name="dbKeyActions"> |
21 <actions> | 50 <actions> |
22 <conditional name="refGenomeSource.geneSource"> | 51 <conditional name="refGenomeSource.geneSource"> |
23 <when value="indexed"> | 52 <when value="indexed"> |
24 <action type="metadata" name="dbkey"> | 53 <action type="metadata" name="dbkey"> |
25 <option type="from_data_table" name="rnastar_index2" column="1" offset="0"> | 54 <option type="from_data_table" name="rnastar_index2_versioned" column="1" offset="0"> |
26 <filter type="param_value" column="0" value="#" compare="startswith" keep="False"/> | 55 <filter type="param_value" column="0" value="#" compare="startswith" keep="False"/> |
27 <filter type="param_value" ref="refGenomeSource.GTFconditional.genomeDir" column="0"/> | 56 <filter type="param_value" ref="refGenomeSource.GTFconditional.genomeDir" column="0"/> |
28 </option> | 57 </option> |
29 </action> | 58 </action> |
30 </when> | 59 </when> |
34 </action> | 63 </action> |
35 </when> | 64 </when> |
36 </conditional> | 65 </conditional> |
37 </actions> | 66 </actions> |
38 </xml> | 67 </xml> |
68 <token name="@TEMPINDEX@"><![CDATA[ | |
69 ## Create temporary index for custom reference | |
70 #if str($refGenomeSource.geneSource) == 'history': | |
71 mkdir -p tempstargenomedir && | |
72 STAR | |
73 --runMode genomeGenerate | |
74 --genomeDir 'tempstargenomedir' | |
75 --genomeFastaFiles '${refGenomeSource.genomeFastaFiles}' | |
76 ## Handle difference between indices with/without annotations | |
77 #if str($refGenomeSource.GTFconditional.GTFselect) == 'with-gtf': | |
78 --sjdbOverhang '${refGenomeSource.GTFconditional.sjdbOverhang}' | |
79 --sjdbGTFfile '${refGenomeSource.GTFconditional.sjdbGTFfile}' | |
80 #if str($refGenomeSource.GTFconditional.sjdbGTFfile.ext) == 'gff3': | |
81 --sjdbGTFtagExonParentTranscript Parent | |
82 #end if | |
83 #end if | |
84 #if str($refGenomeSource.genomeSAindexNbases): | |
85 --genomeSAindexNbases ${refGenomeSource.genomeSAindexNbases} | |
86 #end if | |
87 --runThreadN \${GALAXY_SLOTS:-4} | |
88 && | |
89 #end if | |
90 ]]></token> | |
91 <token name="@REFGENOMEHANDLING" ><![CDATA[ | |
92 --runThreadN \${GALAXY_SLOTS:-4} | |
93 --genomeLoad NoSharedMemory | |
94 --genomeDir | |
95 #if str($refGenomeSource.geneSource) == 'history': | |
96 tempstargenomedir | |
97 #else: | |
98 '${refGenomeSource.GTFconditional.genomeDir.fields.path}' | |
99 ## Handle difference between indices with/without annotations | |
100 #if str($refGenomeSource.GTFconditional.GTFselect) == 'with-gtf': | |
101 #if $refGenomeSource.GTFconditional.sjdbGTFfile: | |
102 --sjdbOverhang $refGenomeSource.GTFconditional.sjdbOverhang | |
103 --sjdbGTFfile '${refGenomeSource.GTFconditional.sjdbGTFfile}' | |
104 #if str($refGenomeSource.GTFconditional.sjdbGTFfile.ext) == 'gff3': | |
105 --sjdbGTFtagExonParentTranscript Parent | |
106 #end if | |
107 #end if | |
108 #end if | |
109 #end if | |
110 ]]></token> | |
111 <xml name="stdio" > | |
112 <stdio> | |
113 <regex match="FATAL error" source="both" level="fatal"/> | |
114 <regex match="EXITING: FATAL INPUT ERROR:" source="both" level="fatal"/> | |
115 <regex match="EXITING: fatal error trying to allocate genome arrays, exception thrown: std::bad_alloc" source="both" level="fatal"/> | |
116 <regex match="\[sam_read1\] missing header\? Abort!" source="both" level="fatal"/> | |
117 </stdio> | |
118 </xml> | |
119 <xml name="refgenomehandling" > | |
120 <conditional name="refGenomeSource"> | |
121 <param name="geneSource" type="select" label="Custom or built-in reference genome" help="Built-ins were indexed using default options"> | |
122 <option value="indexed" selected="true">Use a built-in index</option> | |
123 <option value="history">Use reference genome from history and create temporary index</option> | |
124 </param> | |
125 <when value="indexed"> | |
126 <conditional name="GTFconditional"> | |
127 <param name="GTFselect" type="select" | |
128 label="Reference genome with or without an annotation" | |
129 help="Select the '... with builtin gene-model' option to select from the list of available indexes that were built with splice junction information. Select the '... without builtin gene-model' option to select from the list of available indexes without annotated splice junctions."> | |
130 <option value="without-gtf">use genome reference with builtin gene-model</option> | |
131 <option value="with-gtf">use genome reference without builtin gene-model</option> | |
132 </param> | |
133 <when value="with-gtf"> | |
134 <expand macro="index_selection" with_gene_model="0" /> | |
135 <expand macro="@SJDBOPTIONS@" /> | |
136 </when> | |
137 <when value="without-gtf"> | |
138 <expand macro="index_selection" with_gene_model="1" /> | |
139 </when> | |
140 </conditional> | |
141 </when> | |
142 <when value="history"> | |
143 <param argument="--genomeFastaFiles" type="data" format="fasta" label="Select a reference genome" /> | |
144 <!-- Currently, this parameter is not exposed in the wrapper, | |
145 but used only in the tests to avoid excessive index sizes for | |
146 the tiny test genomes. --> | |
147 <param name="genomeSAindexNbases" type="hidden" value="" /> | |
148 <conditional name="GTFconditional"> | |
149 <param name="GTFselect" type="select" | |
150 label="Build index with our without known splice junctions annotation" | |
151 help="To build an index with known splice junctions annotated, you will have to provide a GTF or GFF3 dataset that describes the gene models (the location of genes, transcripts and exons) known for the reference genome."> | |
152 <option value="without-gtf">build index without gene-model</option> | |
153 <option value="with-gtf">build index with gene-model</option> | |
154 </param> | |
155 <when value="with-gtf"> | |
156 <expand macro="@SJDBOPTIONS@" optional="false"/> | |
157 </when> | |
158 <when value="without-gtf" /> | |
159 </conditional> | |
160 </when> | |
161 </conditional> | |
162 </xml> | |
39 </macros> | 163 </macros> |