Mercurial > repos > iuc > rna_starsolo
annotate tool-data/rnastar_index2x_versioned.loc.sample @ 22:a00cceb45700 draft default tip
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/rgrnastar commit e5d0d8e40525840311dea1d212af911a6eade256
| author | iuc | 
|---|---|
| date | Sat, 31 May 2025 19:53:27 +0000 | 
| parents | eec9494fdafa | 
| children | 
| rev | line source | 
|---|---|
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7
 
e403d27e8f24
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/rgrnastar commit a8e319862d723654c372a6d71e5de76e052586a9"
 
iuc 
parents:  
diff
changeset
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1 #This is a sample file distributed with Galaxy that enables tools | 
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e403d27e8f24
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/rgrnastar commit a8e319862d723654c372a6d71e5de76e052586a9"
 
iuc 
parents:  
diff
changeset
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2 #to use a directory of rna-star indexed sequences data files. | 
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e403d27e8f24
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/rgrnastar commit a8e319862d723654c372a6d71e5de76e052586a9"
 
iuc 
parents:  
diff
changeset
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3 #You will need to create these data files and then create a | 
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e403d27e8f24
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/rgrnastar commit a8e319862d723654c372a6d71e5de76e052586a9"
 
iuc 
parents:  
diff
changeset
 | 
4 #rnastar_index2x_versioned.loc file similar to this one (store it in this | 
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11
 
eec9494fdafa
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/rgrnastar commit 1f8b01701c5b81d155617267a179ba42a0d4a307"
 
iuc 
parents: 
7 
diff
changeset
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5 #directory) that points to the directories in which those files are stored. | 
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7
 
e403d27e8f24
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/rgrnastar commit a8e319862d723654c372a6d71e5de76e052586a9"
 
iuc 
parents:  
diff
changeset
 | 
6 #The rnastar_index2x_versioned.loc file has this format (longer white space | 
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e403d27e8f24
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/rgrnastar commit a8e319862d723654c372a6d71e5de76e052586a9"
 
iuc 
parents:  
diff
changeset
 | 
7 #characters are TAB characters): | 
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e403d27e8f24
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/rgrnastar commit a8e319862d723654c372a6d71e5de76e052586a9"
 
iuc 
parents:  
diff
changeset
 | 
8 # | 
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e403d27e8f24
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/rgrnastar commit a8e319862d723654c372a6d71e5de76e052586a9"
 
iuc 
parents:  
diff
changeset
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9 #<unique_build_id> <dbkey> <display_name> <file_base_path> <with_gene_model> <version> | 
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e403d27e8f24
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/rgrnastar commit a8e319862d723654c372a6d71e5de76e052586a9"
 
iuc 
parents:  
diff
changeset
 | 
10 # | 
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e403d27e8f24
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/rgrnastar commit a8e319862d723654c372a6d71e5de76e052586a9"
 
iuc 
parents:  
diff
changeset
 | 
11 #The <with_gene_model> column should be 1 or 0, indicating whether the index | 
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e403d27e8f24
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/rgrnastar commit a8e319862d723654c372a6d71e5de76e052586a9"
 
iuc 
parents:  
diff
changeset
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12 #was built with annotations (i.e., --sjdbGTFfile and --sjdbOverhang were used) | 
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e403d27e8f24
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/rgrnastar commit a8e319862d723654c372a6d71e5de76e052586a9"
 
iuc 
parents:  
diff
changeset
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13 #or not. | 
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e403d27e8f24
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/rgrnastar commit a8e319862d723654c372a6d71e5de76e052586a9"
 
iuc 
parents:  
diff
changeset
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14 # | 
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e403d27e8f24
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/rgrnastar commit a8e319862d723654c372a6d71e5de76e052586a9"
 
iuc 
parents:  
diff
changeset
 | 
15 #The <version> column indicates the STAR version that introduced the format of | 
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e403d27e8f24
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/rgrnastar commit a8e319862d723654c372a6d71e5de76e052586a9"
 
iuc 
parents:  
diff
changeset
 | 
16 #the index, i.e., the oldest STAR version that could make use of the index. | 
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e403d27e8f24
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/rgrnastar commit a8e319862d723654c372a6d71e5de76e052586a9"
 
iuc 
parents:  
diff
changeset
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17 # | 
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e403d27e8f24
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/rgrnastar commit a8e319862d723654c372a6d71e5de76e052586a9"
 
iuc 
parents:  
diff
changeset
 | 
18 #Note that STAR indices can become quite large. Consequently, it is only | 
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e403d27e8f24
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/rgrnastar commit a8e319862d723654c372a6d71e5de76e052586a9"
 
iuc 
parents:  
diff
changeset
 | 
19 #advisable to create indices with annotations if it's known ahead of time that | 
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e403d27e8f24
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/rgrnastar commit a8e319862d723654c372a6d71e5de76e052586a9"
 
iuc 
parents:  
diff
changeset
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20 #(A) the annotations won't be frequently updated and (B) the read lengths used | 
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e403d27e8f24
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/rgrnastar commit a8e319862d723654c372a6d71e5de76e052586a9"
 
iuc 
parents:  
diff
changeset
 | 
21 #will also rarely vary. If either of these is not the case, it's advisable to | 
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e403d27e8f24
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/rgrnastar commit a8e319862d723654c372a6d71e5de76e052586a9"
 
iuc 
parents:  
diff
changeset
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22 #create indices without annotations and then specify an annotation file and | 
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e403d27e8f24
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/rgrnastar commit a8e319862d723654c372a6d71e5de76e052586a9"
 
iuc 
parents:  
diff
changeset
 | 
23 #maximum read length (minus 1) when running STAR. | 
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e403d27e8f24
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/rgrnastar commit a8e319862d723654c372a6d71e5de76e052586a9"
 
iuc 
parents:  
diff
changeset
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24 # | 
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e403d27e8f24
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/rgrnastar commit a8e319862d723654c372a6d71e5de76e052586a9"
 
iuc 
parents:  
diff
changeset
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25 #hg19 hg19 hg19 full /mnt/galaxyIndices/genomes/hg19/rnastar 0 2.7.1a | 
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e403d27e8f24
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/rgrnastar commit a8e319862d723654c372a6d71e5de76e052586a9"
 
iuc 
parents:  
diff
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26 #hg19Ensembl hg19Ensembl hg19 full with Ensembl annotation /mnt/galaxyIndices/genomes/hg19Ensembl/rnastar 1 2.7.1a | 
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e403d27e8f24
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/rgrnastar commit a8e319862d723654c372a6d71e5de76e052586a9"
 
iuc 
parents:  
diff
changeset
 | 
27 | 
