Mercurial > repos > iuc > rna_starsolo
diff macros.xml @ 10:a6fba3d92531 draft
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/rgrnastar commit d0c9fa48df667ffad1abd71164e6bb1d9cb16bd9"
author | iuc |
---|---|
date | Mon, 15 Mar 2021 13:46:45 +0000 |
parents | ec9cbd6b9a49 |
children | 9ee34ba73ebf |
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--- a/macros.xml Fri Jan 15 17:39:11 2021 +0000 +++ b/macros.xml Mon Mar 15 13:46:45 2021 +0000 @@ -5,7 +5,7 @@ the index versions in sync, but you should manually adjust the +galaxy version number. --> <!-- STAR version to be used --> - <token name="@VERSION@">2.7.7a</token> + <token name="@VERSION@">2.7.8a</token> <!-- STAR index version compatible with this version of STAR This is the STAR version that introduced the index structure expected by the current version. @@ -163,10 +163,7 @@ ]]></token> <xml name="ref_selection"> <param argument="--genomeFastaFiles" type="data" format="fasta" label="Select a reference genome" /> - <!-- Currently, this parameter is not exposed in the wrapper, - but used only in the tests to avoid excessive index sizes for - the tiny test genomes. --> - <param name="genomeSAindexNbases" type="hidden" value="" /> + <param argument="--genomeSAindexNbases" type="integer" min="2" max="16" value="14" label="Length of the SA pre-indexing string" help="Typically between 10 and 15. Longer strings will use much more memory, but allow faster searches. For small genomes, the parameter --genomeSAindexNbases must be scaled down to min(14, log2(GenomeLength)/2 - 1)"/> </xml> <xml name="stdio" > <stdio> @@ -209,8 +206,9 @@ </conditional> </xml> <xml name="umidedup_options"> - <option value="1MM_All" selected="true">All</option> - <option value="1MM_Directional" >Directional</option> + <option value="1MM_All" selected="true">Collapse all UMIs with 1 mismatch distance to each other</option> + <option value="1MM_Directional_UMItools" >Directional method from the UMI-tool</option> + <option value="1MM_Directional" >Directional with stringent UMI deduplication</option> </xml> <xml name="anchor_types"> <option value="0">Read start</option> @@ -225,5 +223,26 @@ <xml name="cb_match_wl_cellranger"> <option value="1MM_multi" selected="true" >Multiple matches (CellRanger 2)</option> <option value="1MM_multi_pseudocounts" >Multiple matches (CellRanger 3)</option> + <option value="1MM_multi_Nbase_pseudocounts" >Multimatching to WL is allowed for CBs with N-bases (CellRanger 3)</option> + </xml> + <xml name="solo_adapter_params"> + <param argument="--soloAdapterSequence" type="text" value="-" label="Adapter sequence to anchor barcodes." > + <sanitizer> + <valid initial="string.digits"> + <add value="-"/> + <add value="A"/> + <add value="T"/> + <add value="C"/> + <add value="G"/> + <add value="N"/> + </valid> + </sanitizer> + </param> + <param argument="--soloAdapterMismatchesNmax" type="integer" min="1" value="1" label="Maximum number of mismatches allowed in adapter sequence" /> + <param argument="--clipAdapterType" type="select" > + <option value="Hamming" selected="true" >Adapter clipping based on Hamming distance</option> + <option value="CellRanger4" >5p and 3p adapter clipping similar to CellRanger4</option> + <option value="None" >No adapter clipping</option> + </param> </xml> </macros>