Mercurial > repos > iuc > samtools_fixmate
diff samtools_fixmate.xml @ 0:bc0cc7bfbfe9 draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools/samtools_fixmate commit 0fe00966500158720fb63dc9b28f6a4ca0d1e1eb
author | iuc |
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date | Fri, 28 Sep 2018 04:28:45 -0400 |
parents | |
children | 595ae1ba934a |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/samtools_fixmate.xml Fri Sep 28 04:28:45 2018 -0400 @@ -0,0 +1,86 @@ +<tool id="samtools_fixmate" name="Samtools fixmate" version="@TOOL_VERSION@"> + <description>fill mate coordinates, ISIZE and mate related flags</description> + <macros> + <import>macros.xml</import> + </macros> + <expand macro="requirements"/> + <expand macro="stdio"/> + <expand macro="version_command"/> + <command><![CDATA[ + @ADDTHREADS@ + ## name sort input + #if not $bamfile.is_of_type('qname_sorted.bam', 'qnamed_input_sorted.bam'): + samtools sort + -@ \$addthreads -m \${GALAXY_MEMORY_MB:-768}M -T sorttemp + -n + -O BAM + -o namesorted.bam + '$bamfile' && + #else: + ln -s '$bamfile' namesorted.bam && + #end if + + samtools fixmate + -@ \$addthreads + $remsec + $noprop + $tempcigar + $addms + -O BAM + namesorted.bam + '$output' + ]]></command> + <inputs> + <param name="bamfile" type="data" format="sam,bam,cram" optional="false" label="Select alignment" help="Set of aligned reads." /> + <param name="remsec" type="boolean" argument="-r" truevalue="-r" falsevalue="" checked="false" label="Remove secondary and unmapped reads" /> + <param name="noprop" type="boolean" argument="-p" truevalue="-p" falsevalue="" checked="false" label="Disable FR proper pair check" /> + <param name="tempcigar" type="boolean" argument="-c" truevalue="-c" falsevalue="" checked="false" label="Add template cigar ct tag" /> + <param name="addms" type="boolean" argument="-m" truevalue="-m" falsevalue="" checked="false" label="Add ms (mate score) tags" help="These are used by markdup to select the best reads to keep." /> + </inputs> + <outputs> + <!--<data name="output" format="sam" />--> + <data name="output" format="qname_sorted.bam"/> + </outputs> + <tests> +<!-- from https://github.com/samtools/samtools/blob/6d79411685d8f0fbb34e123f52d72b63271f4dcb/test/test.pl#L2493--> +<!-- test_cmd($opts,out=>'fixmate/1_coord_sort.sam.expected', err=>'fixmate/1_coord_sort.sam.expected.err', cmd=>"$$opts{bin}/samtools fixmate${threads} -O sam $$opts{path}/fixmate/1_coord_sort.sam -", expect_fail=>1);--> +<test> + <param name="bamfile" value="2_isize_overflow.sam" /> + <output name="output" file="2_isize_overflow.bam.expected" /> +</test> +<!-- test_cmd($opts,out=>'fixmate/2_isize_overflow.bam.expected', cmd=>"$$opts{bin}/samtools fixmate${threads} -O sam $$opts{path}/fixmate/2_isize_overflow.sam -");--> +<test> + <param name="bamfile" value="3_reverse_read_pp_lt.sam" /> + <output name="output" file="3_reverse_read_pp_lt.bam.expected" /> +</test> +<!-- test_cmd($opts,out=>'fixmate/3_reverse_read_pp_lt.bam.expected', cmd=>"$$opts{bin}/samtools fixmate${threads} -O sam $$opts{path}/fixmate/3_reverse_read_pp_lt.sam -");--> +<test> + <param name="bamfile" value="4_reverse_read_pp_equal.sam" /> + <output name="output" file="4_reverse_read_pp_equal.bam.expected" /> +</test> +<!-- test_cmd($opts,out=>'fixmate/4_reverse_read_pp_equal.bam.expected', cmd=>"$$opts{bin}/samtools fixmate${threads} -O sam $$opts{path}/fixmate/4_reverse_read_pp_equal.sam -");--> +<test> + <param name="bamfile" value="5_ct.sam" /> + <param name="tempcigar" value="-c" /> + <output name="output" file="5_ct.bam.expected" /> +</test> +<!-- test_cmd($opts,out=>'fixmate/5_ct.bam.expected', cmd=>"$$opts{bin}/samtools fixmate${threads} -cO sam $$opts{path}/fixmate/5_ct.sam -");--> +<test> + <param name="bamfile" value="6_ct_replace.sam" /> + <param name="tempcigar" value="-c" /> + <output name="output" file="6_ct_replace.bam.expected" /> +</test> +<!-- test_cmd($opts,out=>'fixmate/6_ct_replace.bam.expected', cmd=>"$$opts{bin}/samtools fixmate${threads} -cO sam $$opts{path}/fixmate/6_ct_replace.sam -");--> +<test> + <param name="bamfile" value="7_two_read_mapped.sam" /> + <output name="output" file="7_two_read_mapped.bam.expected" /> +</test> +<!--test_cmd($opts,out=>'fixmate/7_two_read_mapped.bam.expected', cmd=>"$$opts{bin}/samtools fixmate${threads} -O sam $$opts{path}/fixmate/7_two_read_mapped.sam -");--> + </tests> + <help> +**What it does** + +Fill in mate coordinates, ISIZE and mate related flags from a name-sorted alignment. + </help> + <expand macro="citations"/> +</tool>