annotate README.md @ 0:397d2c97af05 draft

planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
author iuc
date Mon, 04 Mar 2019 10:14:25 -0500
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397d2c97af05 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
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1 Scanpy
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2 ======
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4 ## Classification of methods into steps
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6 Steps:
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8 1. Filtering
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10 Methods | Description
397d2c97af05 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
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11 --- | ---
397d2c97af05 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
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12 `pp.filter_cells` | Filter cell outliers based on counts and numbers of genes expressed.
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13 `pp.filter_genes` | Filter genes based on number of cells or counts.
397d2c97af05 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
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14 `pp.filter_genes_dispersion` | Extract highly variable genes
397d2c97af05 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
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15 `pp.highly_variable_genes` | Extract highly variable genes
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16 `pp.subsample` | Subsample to a fraction of the number of observations
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17 `queries.gene_coordinates` | (Could not find...)
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18 `queries.mitochondrial_genes` | Retrieves Mitochondrial gene symbols for specific organism through BioMart for filtering
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20 2. Quality Plots
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22 These are in-between stages used to measure the effectiveness of a Filtering/Normalisation/Conf.Removal stage either after processing or prior to.
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24 Methods | Description | Notes
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25 --- | --- | ---
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26 `pp.calculate_qc_metrics` | Calculate quality control metrics
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27 `pl.violin` | violin plot of features, lib. size, or subsets of.
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28 `pl.stacked_violin` | Same as above but for multiple series of features or cells
397d2c97af05 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
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30 3. Normalization
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32 Methods | Description
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33 --- | ---
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34 `pp.normalize_per_cell` | Normalize total counts per cell
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35 `pp.recipe_zheng17` | Normalization and filtering as of [Zheng17]
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36 `pp.recipe_weinreb17` | Normalization and filtering as of [Weinreb17]
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37 `pp.recipe_seurat` | Normalization and filtering as of Seurat [Satija15]
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38 `pp.log1p` | Logarithmize the data matrix.
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39 `pp.scale` | Scale data to unit variance and zero mean
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40 `pp.sqrt` |
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41 `pp.downsample_counts` | Downsample counts so that each cell has no more than target_counts
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43 4. Conf. removal
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45 Methods | Description
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46 --- | ---
397d2c97af05 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
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47 `pp.regress_out` | Regress out unwanted sources of variation
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48 `pp.mnn_correct` | Correct batch effects by matching mutual nearest neighbors
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49 `pp.dca` | Deep count autoencoder to denoise the data
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50 `pp.magic` | Markov Affinity-based Graph Imputation of Cells (MAGIC) API to denoise
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51 `tl.sim` | Simulate dynamic gene expression data [Wittman09]
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52 `pp.calculate_qc_metrics` | Calculate quality control metrics
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53 `tl.score_genes` | Score a set of genes
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54 `tl.score_genes_cell_cycle` | Score cell cycle genes
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55 `tl.cyclone` | Assigns scores and predicted class to observations based on cell-cycle genes [Scialdone15]
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56 `tl.sandbag` | Calculates pairs of genes serving as markers for each cell-cycle phase [Scialdone15]
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57
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58 5. Clustering and Heatmaps
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59
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60 Methods | Description
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61 --- | ---
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62 `tl.leiden` | Cluster cells into subgroups [Traag18] [Levine15]
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63 `tl.louvain` | Cluster cells into subgroups [Blondel08] [Levine15] [Traag17]
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64 `tl.pca` | Principal component analysis
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65 `pp.pca` | Principal component analysis (appears to be the same func...)
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66 `tl.diffmap` | Diffusion Maps
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67 `tl.tsne` | t-SNE
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68 `tl.umap` | Embed the neighborhood graph using UMAP
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69 `tl.phate` | PHATE
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70 `pp.neighbors` | Compute a neighborhood graph of observations
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71 `tl.rank_genes_groups` | Rank genes for characterizing groups
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72 `pl.rank_genes_groups` |
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73 `pl.rank_genes_groups_dotplot` |
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74 `pl.rank_genes_groups_heatmap` |
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75 `pl.rank_genes_groups_matrixplot` |
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76 `pl.rank_genes_groups_stacked_violin` |
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77 `pl.rank_genes_groups_violin` |
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78 `pl.matrix_plot` |
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79 `pl.heatmap` |
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80 `pl.highest_expr_genes` |
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81 `pl.diffmap` |
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82
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83 6. Cluster Inspection and plotting
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84
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85 Methods that draw out the clusters computed in the previous stage, not heatmap or pseudotime related.
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86
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87 Methods | Description
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88 --- | ---
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89 `pl.clustermap` |
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90 `pl.phate` |
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91 `pl.dotplot` |
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92 `pl.draw_graph` | (really general purpose, would not implement directly)
397d2c97af05 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
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93 `pl.filter_genes_dispersion` | (depreciated for 'highly_variable_genes')
397d2c97af05 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
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94 `pl.matrix` | (could not find in API)
397d2c97af05 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
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95 `pl.pca` |
397d2c97af05 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
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96 `pl.pca_loadings` |
397d2c97af05 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
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97 `pl.pca_overview` |
397d2c97af05 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
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98 `pl.pca_variance_ratio` |
397d2c97af05 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
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99 `pl.ranking` | (not sure what this does...)
397d2c97af05 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
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100 `pl.scatter` | ([very general purpose](https://icb-scanpy.readthedocs-hosted.com/en/latest/api/scanpy.api.pl.scatter.html), would not implement directly)
397d2c97af05 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
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101 `pl.set_rcParams_defaults` |
397d2c97af05 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
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102 `pl.set_rcParams_scanpy` |
397d2c97af05 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
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103 `pl.sim` |
397d2c97af05 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
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104 `pl.tsne` |
397d2c97af05 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
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105 `pl.umap` |
397d2c97af05 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
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106
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107 7. Branch/Between-Cluster Inspection
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108
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109 Pseudotime analysis, relies on initial clustering.
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110
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111 Methods | Description
397d2c97af05 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
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112 --- | ---
397d2c97af05 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
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113 `tl.dpt` | Infer progression of cells through geodesic distance along the graph [Haghverdi16] [Wolf17i]
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114 `pl.dpt_groups_pseudotime` |
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115 `pl.dpt_timeseries` |
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116 `tl.paga_compare_paths` |
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117 `tl.paga_degrees` |
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118 `tl.paga_expression_entropies` |
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119 `tl.paga` | Generate cellular maps of differentiation manifolds with complex topologies [Wolf17i]
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120 `pl.paga` |
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121 `pl.paga_adjacency` |
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122 `pl.paga_compare` |
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123 `pl.paga_path` |
397d2c97af05 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
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124 `pl.timeseries` |
397d2c97af05 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
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125 `pl.timeseries_as_heatmap` |
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126 `pl.timeseries_subplot` |
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127
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128
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129 Methods to sort | Description
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130 --- | ---
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131 `tl.ROC_AUC_analysis` | (could not find in API)
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132 `tl.correlation_matrix` | (could not find in API)
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133 `rtools.mnn_concatenate` | (could not find in API)
397d2c97af05 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
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134 `utils.compute_association_matrix_of_groups` | (could not find in API)
397d2c97af05 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
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135 `utils.cross_entropy_neighbors_in_rep` | (could not find in API)
397d2c97af05 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
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136 `utils.merge_groups` | (could not find in API)
397d2c97af05 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
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137 `utils.plot_category_association` | (could not find in API)
397d2c97af05 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
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138 `utils.select_groups` | (could not find in API)