# HG changeset patch # User iuc # Date 1583867258 14400 # Node ID 649c86cedcb442ed41cb5330c8f7ad77d17a7664 # Parent e59d83af97a19689c3a37538575f49f0c3713ad1 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/schicexplorer commit 72e1e90ac05a32dbd6fc675073429c0086048b18" diff -r e59d83af97a1 -r 649c86cedcb4 macros.xml --- a/macros.xml Thu Jan 23 15:59:42 2020 -0500 +++ b/macros.xml Tue Mar 10 15:07:38 2020 -0400 @@ -1,6 +1,6 @@ \${GALAXY_SLOTS:-4} - 1 + 4 @@ -9,8 +9,8 @@ @BINARY@ --version - - + @@ -18,7 +18,7 @@ label="Matricies to compute on" multiple="true"/> - + diff -r e59d83af97a1 -r 649c86cedcb4 scHicDemultiplex.xml --- a/scHicDemultiplex.xml Thu Jan 23 15:59:42 2020 -0500 +++ b/scHicDemultiplex.xml Tue Mar 10 15:07:38 2020 -0400 @@ -19,6 +19,12 @@ --bufferSize $bufferSize --threads @THREADS@ + && cd outputFiles + && mkdir forward + && mkdir reverse + && mv *R1.fastq.gz forward + && mv *R2.fastq.gz reverse + ]]> @@ -29,8 +35,11 @@ - - + + + + + @@ -39,57 +48,60 @@ + + + + + + + + + + + + + + + + + + + + + + + + + + + + - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - + + + + + + + + + + + + + + + + + + + + + + + + + @@ -99,7 +111,9 @@ ================================= scHicDemultiplex demultiplexes fastq files from Nagano 2017: "Cell-cycle dynamics of chromosomal organization at single-cell resolution" according their barcodes to -a seperated forward and reverse strand fastq files per cell. +a seperated forward and reverse strand fastq files per cell. For other datasets, a third-party demultiplexing strategy must be used. + +Afterwards, the demultiplexed mapped data can be used with HiCExplorer `hicBuildMatrix` to create single cell .cool matrices that must be stored in a .scool file using `scHicMergeToSCool`, in order to be used for downstream analyses in the scHiCExplorer suite. 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distance): 0 -12 samples passed the quality control. Please consider matrices with a low read coverage may be the matrices with a low density and overlap therefore. \ No newline at end of file +10 samples passed the quality control. Please consider matrices with a low read coverage may be the matrices with a low density and overlap therefore. \ No newline at end of file diff -r e59d83af97a1 -r 649c86cedcb4 test-data/test_matrix.mcool Binary file test-data/test_matrix.mcool has changed diff -r e59d83af97a1 -r 649c86cedcb4 test-data/test_matrix.scool Binary file test-data/test_matrix.scool has changed