Mercurial > repos > iuc > seqprep
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/seqprep commit 06828a419d1bf8546382b7c687a76b65a577e736
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/README.md Thu Feb 15 14:23:13 2024 +0000 @@ -0,0 +1,15 @@ +# SeqPrep Tool Documentation + +## Overview + +SeqPrep is a bioinformatics tool primarily used for processing paired-end Illumina reads. It excels in merging overlapping reads and trimming adapter sequences, making it a valuable asset in genomic sequence analysis. Its efficient handling of paired-end data ensures high-quality preparation for downstream analysis. + +## Key Features + +- **Read Merging**: Seamlessly merges overlapping paired-end Illumina reads into longer, single reads. +- **Adapter Trimming**: Efficiently trims adapter sequences from read ends to improve data quality. +- **Quality Control**: Offers options for quality score cutoff and minimum read length, enhancing the integrity of output data. + +## Usage + +For a detailed tutorial on how to use SeqPrep, please refer to the [SeqPrep Tutorial](https://github.com/jstjohn/SeqPrep/tree/master).
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/macros.xml Thu Feb 15 14:23:13 2024 +0000 @@ -0,0 +1,35 @@ +<macros> + <token name="@TOOL_VERSION@">1.3.2</token> + <token name="@VERSION_SUFFIX@">0</token> + <xml name="requirements"> + <requirements> + <requirement type="package" version="@TOOL_VERSION@">seqprep</requirement> + </requirements> + </xml> + <xml name="biotools"> + <xrefs> + <xref type="bio.tools">seqprep</xref> + </xrefs> + </xml> + <xml name="creators"> + <creator> + <person givenName="John" familyName="St. John" url="https://github.com/jstjohn" /> + <person givenName="Lionel" familyName="Guy" /> + <person givenName="John" familyName="Chilton" url="https://github.com/jmchilton" /> + <person givenName="Albert" familyName="Ratschinski" url="https://github.com/Alby-Git" /> + </creator> + </xml> + <xml name="citations"> + <citations> + <citation type="bibtex"> + @online{seqpre, + author = {St John, John}, + title = {seqprep}, + year = 2016, + url = {https://github.com/jstjohn/SeqPrep}, + urldate = {2016-10-04} + } + </citation> + </citations> + </xml> +</macros>
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/seqprep.xml Thu Feb 15 14:23:13 2024 +0000 @@ -0,0 +1,325 @@ +<tool id="seqprep" name="Merging paired-end Illumina reads (SeqPrep)" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="22.05"> + <description>Merge and Trim Adapter Sequences from Paired-End Illumina Reads</description> + <macros> + <import>macros.xml</import> + </macros> + <expand macro="biotools"/> + <expand macro="requirements"/> + <expand macro="creators"/> + <command detect_errors="exit_code"><![CDATA[ + SeqPrep + -f '${input1}' + -r '${input2}' + -1 '${output1}' + -2 '${output2}' + + #if $merge_reads + -s '${merged}' + #end if + + ## General Arguments ## + #if $general_options.first_read_discarded + -3 '${general_options.first_read_discarded}' + #end if + #if $general_options.second_read_discarded + -4 '${general_options.second_read_discarded}' + #end if + #if $general_options.phred64 + -6 '${general_options.phred64}' + #end if + #if $general_options.quality_cutoff + -q '${general_options.quality_cutoff}' + #end if + #if $general_options.min_length + -L '${general_options.min_length}' + #end if + + ## Additional Adapter/Primer Trimming Arguments ## + #if $trimming_options.adapter_a + -A '${trimming_options.adapter_a}' + #end if + #if $trimming_options.adapter_b + -B '${trimming_options.adapter_b}' + #end if + #if $trimming_options.adapter_overlap + -O '${trimming_options.adapter_overlap}' + #end if + #if $trimming_options.max_mismatch_fraction + -M '${trimming_options.max_mismatch_fraction}' + #end if + #if $trimming_options.min_match_fraction + -N '${trimming_options.min_match_fraction}' + #end if + #if $trimming_options.adapter_bandwidth + -b '${trimming_options.adapter_bandwidth}' + #end if + #if $trimming_options.gap_open + -Q '${trimming_options.gap_open}' + #end if + #if $trimming_options.gap_extend + -t '${trimming_options.gap_extend}' + #end if + #if $trimming_options.gap_end + -e '${trimming_options.gap_end}' + #end if + #if $trimming_options.local_alignment_score + -Z '${trimming_options.local_alignment_score}' + #end if + #if $trimming_options.read_alignment_bandwidth + -w '${trimming_options.read_alignment_bandwidth}' + #end if + #if $trimming_options.read_alignment_gap_open + -W '${trimming_options.read_alignment_gap_open}' + #end if + #if $trimming_options.read_alignment_gap_extend + -p '${trimming_options.read_alignment_gap_extend}' + #end if + #if $trimming_options.read_alignment_gap_end + -P '${trimming_options.read_alignment_gap_end}' + #end if + #if $trimming_options.read_alignment_max_gap_fraction + -X '${trimming_options.read_alignment_max_gap_fraction}' + #end if + + ## Additional Arguments for Merging ## + #if $merging_options.maximum_quality_score + -y '${merging_options.maximum_quality_score}' + #end if + #if $merging_options.print_overhang + -g '${merging_options.print_overhang}' + #end if + #if $merging_options.min_base_pair_overlap + -o '${merging_options.min_base_pair_overlap}' + #end if + #if $merging_options.max_mismatch_fraction + -m '${merging_options.max_mismatch_fraction}' + #end if + #if $merging_options.min_match_fraction + -n '${merging_options.min_match_fraction}' + #end if + ]]></command> + <inputs> + <param name="input1" type="data" format="fastq" label="First Read Input" help="Select the FASTQ file containing the first set of paired-end reads." /> + <param name="input2" type="data" format="fastq" label="Second Read Input" help="Select the FASTQ file containing the second set of paired-end reads." /> + <param name="merge_reads" type="boolean" truevalue="true" falsevalue="false" checked="true" label="Merge Reads" help="Enable this to merge overlapping reads from the provided paired-end FASTQ files." /> + + <!-- Section for General Arguments --> + <section name="general_options" title="General Arguments (Optional)" expanded="false"> + <param name="first_read_discarded" argument="-3" type="text" optional="true" label="First Read Discarded FASTQ Filename" help="first read discarded fastq filename" /> + <param name="second_read_discarded" argument="-4" type="text" optional="true" label="Second Read Discarded FASTQ Filename" help="second read discarded fastq filename" /> + <param name="phred64" argument="-6" type="boolean" truevalue="-6" falsevalue="" checked="false" label="Input Sequence is in Phred+64 Format" help="Input sequence is in phred+64 rather than phred+33 format, the output will still be phred+33"/> + <param name="quality_cutoff" argument="-q" type="integer" optional="true" value="13" label="Quality Score Cutoff" help="Quality score cutoff for mismatches to be counted in overlap" /> + <param name="min_length" argument="-L" type="integer" optional="true" value="30" label="Minimum Length of Reads" help="Minimum length of a trimmed or merged read to print it" /> + </section> + + <!-- Section for Additional Adapter/Primer Trimming Arguments --> + <section name="trimming_options" title="Additional Adapter/Primer Trimming Arguments" expanded="false"> + <param name="adapter_a" argument="-A" label="Adapter Sequence A" type="text" optional="true" value="AGATCGGAAGAGCGGTTCAG" help="Forward read primer/adapter sequence to trim as it would appear at the end of a read" /> + <param name="adapter_b" argument="-B" label="Adapter Sequence B" type="text" optional="true" value="AGATCGGAAGAGCGTCGTGT" help="Reverse read primer/adapter sequence to trim as it would appear at the end of a read" /> + <param name="adapter_overlap" argument="-O" label="Minimum Overall Base Pair Overlap with Adapter" type="integer" value="10" optional="true" help="minimum overall base pair overlap with adapter sequence to trim" /> + <param name="max_mismatch_fraction" argument="-M" label="Maximum Fraction of Good Quality Mismatching Bases" type="float" value="0.02" optional="true" help="maximum fraction of good quality mismatching bases for primer/adapter overlap" /> + <param name="min_match_fraction" argument="-N" label="Minimum Fraction of Matching Bases" type="float" value="0.87" optional="true" help="minimum fraction of matching bases for primer/adapter overlap" /> + <param name="adapter_bandwidth" argument="-b" label="Adapter Alignment Band-width" type="integer" value="50" optional="true" /> + <param name="gap_open" argument="-Q" label="Adapter Alignment Gap-Open" type="integer" value="8" optional="true" /> + <param name="gap_extend" argument="-t" label="Adapter Alignment Gap-Extension" type="integer" value="2" optional="true" /> + <param name="gap_end" argument="-e" label="Adapter Alignment Gap-End" type="integer" value="2" optional="true" /> + <param name="local_alignment_score" argument="-Z" label="Minimum Local Alignment Score Cutoff" type="integer" value="26" optional="true" help="Adapter alignment minimum local alignment score cutoff [roughly (2*num_hits) - (num_gaps*gap_open) - (num_gaps*gap_close) - (gap_len*gap_extend) - (2*num_mismatches)]" /> + <param name="read_alignment_bandwidth" argument="-w" label="Read Alignment Band-width" type="integer" value="50" optional="true" /> + <param name="read_alignment_gap_open" argument="-W" label="Read Alignment Gap-Open" type="integer" value="26" optional="true" /> + <param name="read_alignment_gap_extend" argument="-p" label="Read Alignment Gap-Extension" type="integer" value="9" optional="true" /> + <param name="read_alignment_gap_end" argument="-P" label="Read Alignment Gap-End" type="integer" value="5" optional="true" help="read alignment maximum fraction gap cutoff" /> + <param name="read_alignment_max_gap_fraction" argument="-X" label="Read Alignment Maximum Fraction Gap Cutoff" type="float" value="0.125" optional="true" /> + </section> + + <!-- Section for Optional Arguments for Merging: --> + <section name="merging_options" title="Optional Arguments for Merging" expanded="false"> + <param name="maximum_quality_score" argument="-y" label="Maximum Quality Score in Output" type="text" optional="true" help="Maximum quality score in output (phred 33), default = ']'"/> + <param name="print_overhang" argument="-g" type="boolean" truevalue="-g" falsevalue="" checked="false" label="Print Overhang When Adapters Are Present and Stripped" help="Use this if reads are different lengths"/> + <param name="min_base_pair_overlap" argument="-o" type="integer" optional="true" value="15" label="Minimum Overall Base Pair Overlap" help="Minimum overall base pair overlap to merge two reads"/> + <param name="max_mismatch_fraction" argument="-m" type="float" optional="true" value="0.02" label="Maximum Fraction of Good Quality Mismatching Bases" help="Maximum fraction of good quality mismatching bases to overlap reads"/> + <param name="min_match_fraction" argument="-n" type="float" optional="true" value="0.9" label="Minimum Fraction of Matching Bases" help="Minimum fraction of matching bases to overlap reads"/> + </section> + </inputs> + <outputs> + <data format="fastq.gz" name="output1" label="${tool.name} on ${on_string}: First Read Output"> + <filter>output_all</filter> + </data> + <data format="fastq.gz" name="output2" label="${tool.name} on ${on_string}: Second Read Output"> + <filter>output_all</filter> + </data> + <data format="fastq.gz" name="merged" label="${tool.name} on ${on_string}: Merged Reads"> + <filter>merge_reads</filter> + </data> + </outputs> + <tests> + <!-- Test default inputs #1 --> + <test expect_num_outputs="3"> + <param name="input1" value="input1.fq" /> + <param name="input2" value="input2.fq" /> + <param name="merge_reads" value="true" /> + + <!-- Section for General Arguments --> + <section name="general_options" > + <param name="quality_cutoff" value="13" /> + <param name="min_length" value="30" /> + </section> + + <!-- Section for Additional Adapter/Primer Trimming Arguments --> + <section name="trimming_options"> + <param name="adapter_a" value="AGATCGGAAGAGCGGTTCAG" /> + <param name="adapter_b" value="AGATCGGAAGAGCGTCGTGT" /> + <param name="adapter_overlap" value="10" /> + <param name="max_mismatch_fraction" value="0.02" /> + <param name="min_match_fraction" value="0.87" /> + <param name="adapter_bandwidth" value="50" /> + <param name="gap_open" value="8" /> + <param name="gap_extend" value="2" /> + <param name="gap_end" value="2" /> + <param name="local_alignment_score" value="26" /> + <param name="read_alignment_bandwidth" value="50" /> + <param name="read_alignment_gap_open" value="26" /> + <param name="read_alignment_gap_extend" value="9" /> + <param name="read_alignment_gap_end" value="5" /> + <param name="read_alignment_max_gap_fraction" value="0.125" /> + </section> + <output name="output1" file="output1.fq.gz" /> + <output name="output2" file="output2.fq.gz" /> + <output name="merged" file="merged_output.fq.gz" /> + </test> + + <!-- Without Merging, Two Outputs #2 --> + <test expect_num_outputs="2"> + <param name="input1" value="input1.fq" /> + <param name="input2" value="input2.fq" /> + <param name="merge_reads" value="false" /> + + <!-- Section for General Arguments --> + <section name="general_options" > + <param name="quality_cutoff" value="13" /> + <param name="min_length" value="30" /> + </section> + + <!-- Section for Additional Adapter/Primer Trimming Arguments --> + <section name="trimming_options"> + <param name="adapter_a" value="AGATCGGAAGAGCGGTTCAG" /> + <param name="adapter_b" value="AGATCGGAAGAGCGTCGTGT" /> + <param name="adapter_overlap" value="10" /> + <param name="max_mismatch_fraction" value="0.02" /> + <param name="min_match_fraction" value="0.87" /> + <param name="adapter_bandwidth" value="50" /> + <param name="gap_open" value="8" /> + <param name="gap_extend" value="2" /> + <param name="gap_end" value="2" /> + <param name="local_alignment_score" value="26" /> + <param name="read_alignment_bandwidth" value="50" /> + <param name="read_alignment_gap_open" value="26" /> + <param name="read_alignment_gap_extend" value="9" /> + <param name="read_alignment_gap_end" value="5" /> + <param name="read_alignment_max_gap_fraction" value="0.125" /> + </section> + <output name="output1" file="outputNoMerge1.fq.gz" /> + <output name="output2" file="outputNoMerge2.fq.gz" /> + </test> + <!-- Test with Empty Input Files #3 --> + <test expect_num_outputs="3"> + <param name="input1" value="empty1.fq" /> + <param name="input2" value="empty2.fq" /> + <param name="merge_reads" value="true" /> + + <!-- Section for General Arguments --> + <section name="general_options" > + <param name="quality_cutoff" value="13" /> + <param name="min_length" value="30" /> + </section> + + <!-- Section for Additional Adapter/Primer Trimming Arguments --> + <section name="trimming_options"> + <param name="adapter_a" value="AGATCGGAAGAGCGGTTCAG" /> + <param name="adapter_b" value="AGATCGGAAGAGCGTCGTGT" /> + <param name="adapter_overlap" value="10" /> + <param name="max_mismatch_fraction" value="0.02" /> + <param name="min_match_fraction" value="0.87" /> + <param name="adapter_bandwidth" value="50" /> + <param name="gap_open" value="8" /> + <param name="gap_extend" value="2" /> + <param name="gap_end" value="2" /> + <param name="local_alignment_score" value="26" /> + <param name="read_alignment_bandwidth" value="50" /> + <param name="read_alignment_gap_open" value="26" /> + <param name="read_alignment_gap_extend" value="9" /> + <param name="read_alignment_gap_end" value="5" /> + <param name="read_alignment_max_gap_fraction" value="0.125" /> + </section> + <output name="output1" file="empty_output1.fq.gz" /> + <output name="output2" file="empty_output2.fq.gz" /> + <output name="merged" file="empty_merged_output.fq.gz" /> + </test> + + <!-- Advanced Functional Tests --> + <!-- General Arguments Test #4 --> + <test expect_num_outputs="2"> + <param name="input1" value="input1.fq" /> + <param name="input2" value="input2.fq" /> + <param name="merge_reads" value="false" /> + + <param name="quality_cutoff" value="15" /> + <param name="min_length" value="25" /> + <output name="output1" file="output1_general_args.fq.gz" /> + <output name="output2" file="output2_general_args.fq.gz" /> + </test> + + <!-- Adapter/Primer Trimming Arguements Test #5 --> + <test expect_num_outputs="2"> + <param name="input1" value="input1.fq" /> + <param name="input2" value="input2.fq" /> + <param name="merge_reads" value="false" /> + <section name="trimming_options"> + <param name="adapter_a" value="ACTGACTG" /> + <param name="adapter_b" value="GTGACTGA" /> + <param name="adapter_overlap" value="12" /> + <param name="max_mismatch_fraction" value="0.03" /> + <param name="min_match_fraction" value="0.85" /> + <param name="adapter_bandwidth" value="55" /> + <param name="gap_open" value="10" /> + <param name="gap_extend" value="3" /> + <param name="gap_end" value="3" /> + <param name="local_alignment_score" value="28" /> + </section> + <output name="output1" file="output1_adapter_trim.fq.gz" /> + <output name="output2" file="output2_adapter_trim.fq.gz" /> + </test> + <!-- Test with gzipped input files #6 --> + <test expect_num_outputs="3"> + <param name="input1" value="input1.fastq.gz" /> + <param name="input2" value="input2.fastq.gz" /> + <param name="merge_reads" value="true" /> + <output name="output1" file="output1_from_gzipped.fq.gz" /> + <output name="output2" file="output2_from_gzipped.fq.gz" /> + <output name="merged" file="merged_output_from_gzipped.fq.gz" /> + </test> + </tests> + <help><![CDATA[ + **SeqPrep** + + SeqPrep is a versatile tool designed for merging overlapping paired-end Illumina reads into a single, longer read. Additionally, it offers the functionality to trim adapter sequences from reads, making it + a needful tool for preprocessing Illumina sequencing data. + + **Usage** + + To utilize SeqPrep, start by selecting your input FASTQ files: one for the first set of reads and another for the second set. SeqPrep provides several options to customize your data processing: + + - **Adapter Sequences:** You can provide specific sequences for adapter trimming if they are known. SeqPrep will remove these sequences from the reads. + - **Quality Score Cutoff:** Set a threshold for the quality score. Reads with quality scores below this threshold can be discarded or trimmed. + - **Minimum Read Length:** Define the minimum length for reads to be retained after trimming. Reads shorter than this length will be discarded. + + If the merging feature is enabled, SeqPrep will combine overlapping reads into longer sequences, thereby enhancing the data quality for downstream analysis. + + **Outputs** + + SeqPrep generates outputs in gzipped FASTQ format. + + See more details on SeqPrep github repository: https://github.com/jstjohn/SeqPrep + + ]]></help> + <expand macro="citations"/> +</tool>
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/input1.fq Thu Feb 15 14:23:13 2024 +0000 @@ -0,0 +1,240 @@ +@SimSeq_1/229/1 +TAGGAGGCTTGGGGTTAGGAATTGGTCAAATAGGTTTATGTTCAAGGTCAGGGTCATGTTTTAATAGTTATGGCTTTGTAAGGGGTTGGGCTGCTTGTGA ++ +E=AGEFGGGFFEDDGGGECGDADF>DEF?EECAFFEBF:=B2EGGEGF#ABAF7BGFC:FC??:D:?FCD<A??EB5E:G##EEDAB;###.#@EE?BD# +@SimSeq_2/49/1 +AAAGAAATTAGGTTTAAATTCCAAGCCGAGGGCCTTCAAAGCCCTAAATAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAAAAAAAAAAAAAA ++ +F?A?BEEDAGAGFFGBFDE>E=G;FD=ED<CFGDGEGFG7DF5DFGBEGBCBEFCBCEDC+CEBE7D##?,=#5BD+D#:?EGC::#5,#E5=9+D#EF# +@SimSeq_3/149/1 +GAAAATTAATAGCACTATCGTCCATTGCCCAAATAGCATGAATTATCGTAATTATTAAATACGCACCATCACTAGCCATCCTAACCTTCTACATCTGCTC ++ +EDGBGFEEG>:FGD=GG5F?CFEEEFED,?:?D:GFGDEEFDEEDEDFFAEAE@?E>BGEFE3EA@1GED#=#-5:CEAC#?4DBGE?F#FAFD#C#C#G +@SimSeq_4/197/1 +CGGGGGCCTAACTGGGATTGTCCTAGCCAACTCCTCACTAGACATTGTCCTCCACGACACGTATTATGTAGTGGCCCACTTCCACTACGTACTCTCAATA ++ +GDEGGDEGFGGDEGCFE>F>E;G>F@CFGGGFBGEDGDEC@GGEFG>ACBEFGGEE?F?@(G7GEFCG#E#B@A?CE;FE?G:3#7:B?=,F;D@>A##D +@SimSeq_5/40/1 +ACCCCTCTCAGAGGAGGATGGAGCATGGTGGTATTGCGGCAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAAAAAAAAAAAAAAAAAAAAAGA ++ +CFGGD>AFGGGFGEEDGGE?EFDGEGBFEBED+FBG5GGFE:BEAADABEF@?EEEBBF;+DDEDD3=E#F2#)G*#C>>F3>E#@C#?4<G#-BE:?#7 +@SimSeq_6/190/1 +TAACTAAATATTTGTGAGATCGAAGCTCGCCTGTTTATTGAGGTTTTAGCTTAATTAAAGTTTCTGAGTTGCACTTTGAAGATGCCGGATAGAGTCCTGC ++ +DFD:DAE?GE0FDCAFF:GE>DFF;G6G@FGGEEGFD@EGAED=G7E1G:EFGEFD>BGD@:DE?#;(G3=8#:DDBD6#AG##E#@E:8BGG#/DG#<B +@SimSeq_7/178/1 +TAAATCCCATTCCACCCCTACTTCACCATAAAAGACGCCCTAGGAGCAGCACTAGCTGCCTCCTCACTACTCATCTTAGCTCTCTACCTACCAGCCCTAT ++ +:BFCBD@GGFEFGF>AGDDF5EGGFAGGD@DFFDD@G??GF>BEBDE4EGAEFADFE@G:A#GFD=C#BBG#BGF#CEC=F>8:#E<FG5E>E=5DE#:# +@SimSeq_8/18/1 +AATCCTCCCTCCCCTTATAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA ++ +@A8GG?DDFEFCBGGGADFE<7EEFDG>>EGGEDBGAEDG:G8DFF@FG5GECCCEF*=BC=,DC?C>CE#D:BD0#=3ED@F#E(AF5GAE4GGF@:,= +@SimSeq_9/103/1 +GTTGTCCGGTTACTTTAATAGTCAGGCAGGGGTTATTGGTTTCGTCTATGAGGTATAGGGTGCGTAGTGATGGTAGGGCAATTGAGATAAGGACAAAGGC ++ +F;GFDE@?;GF?:5E:CGCGD?@D=GFCEEDC7=EAD#F5G:EGACFDE:*C:FA=BA*<AF;EG=D=CEE7B54C@?C9:=FFB@AG:#E#AB-#DC#F +@SimSeq_a/334/1 +TTCAAGAAGTAGAAGTAATCTGAACCGTCCTACCAGCCATTGTCCTTATCTCAATTGCCCTACCAGCACTACGCACCCTATACCTCAGAGACGAAACCAA ++ +GG?>AGFGEEFFDGFGCFGDFEGC?F:DEBEGGAGEGEE>EEGAFB)F>EBD-FAA>GG?GFG3B#B6G:EG6;>DEG#2G?GC?D##)GB=#D###ED# +@SimSeq_b/247/1 +TAACAATAAGGGAGATTCAAACGATTAGGAAGGTTGTTAGTCAAGGTTCTGGGTTTAGTTGTGGCATGGTCACTAAGGGGGAGGGGGTAATACTAGTCCC ++ +G?DGG?B;GC?GEA?E7AACD@EEDE:GE:=GF??6EEGDFGFFBDFBG0EF.DB@D@EE5A@#E2#8ADA6DEE=@3EDEC#C:CCB#EF?FD=#??5# +@SimSeq_c/0/1 +AGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA ++ +DFBCGCG7B?BG=CEEEFGGEDBGFAEEGGEFADGFD#:ED>/GG:G:BDDFAD:=EG5@EGDBDE>>8D,BDB3F@BGB#BDD#BB:EE:AD:#DD##> +@SimSeq_d/127/1 +TTAAGGCTTAAGTAGATGGGGCAGGTTCCTATGCTGAGTCAATGTCAAGTGAATGAGAGGGGAACTATGTTGTTGTAAGTTAATAAAATAAGGGGAGGGA ++ +BFGCCG?E?EG2EBEGGGGCDGFED:FGDDGDGEEF+CGADDGG=G=ADG?=BE5=EGA:F3DAD>ADE@8DG#E#:A#29BF?AEFEED>=;E#DCC?: +@SimSeq_e/84/1 +CCCCTGTTGTATTTCAACTATAAAAACCCATGACCCACCAACTACGAAAATCCCACCCAATCATTAAACTCATCAACCGCTCCCAGATCGGAAGAGCGGT ++ +BEEFFCFGGGEBG?GE9G>GFEGGCGFEFGBE<EFG>GEGEG@FGBFDE?F-;GF#D#E;@BD?=:??G#DDCF?DGEDDAGBCAC6#F?C#F#475#B# +@SimSeq_f/64/1 +CTCAGGATTCTTTTCCAAAGACGCTATTATTGAAGCACTAAACACCTCTTATATTAACGCCTGTAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACC ++ +ADG9EGGFDC=DGGGF=DFBDGAGG;B5GGDFA=CCFFBGGF<B85DFGAFGAE#1:DCGE?#EB??584F*?BEDAGDFB#D#EG85?EE,E=E6#C:A +@SimSeq_10/154/1 +CACATTCGTAAGGGGATAATTTTTCTGGGTCGGGTGATGTTTTGGCAGTCAGTAGGTGTAGGGCGACTACGATTGAAACGGTGATTGAGGATATTGTGAG ++ +FFEGGCG?ABFBDFGFGGBBGFEG?#DA?9GDGBCCGFEF=DBF#FDG>FEGCD#EA'BF==DGD6#3A@B5?GC#F.G:,DFGCEFF?:5;:A7#C#F= +@SimSeq_11/66/1 +TGCTCGAACCCATAAGAAACTTATGGTTAGTAGGAGAGCCTTGCTTATTAGGGCAATGGTGAATAGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGA ++ +GG;EBGGEGEDCDGGFDED?,DDG?GDGEFGFFD@G;EA@EAEGG)GCDA#CEF7ADAGB=EBEGF#A?G?<ACCCFEDD?#F:B>E#FAA##9G###?> +@SimSeq_12/241/1 +ATGCCGGATAGAGTCCTGCAAACCTTAAATCAAGAAATGGGGGGTTGTTTACTCCCATTTAGGGCTTTGAAGGCCCTCGGCTTGGAATTTACACCTAATT ++ +DGDDE8EGGGGGDGGEEDDE4EDEDCDF?EDBGF?=-AGGB:GFCBGE=?AFBGFAD9B@DAE:F0@>D@C=B=CGC=G#D7??FADBCC#:#:#>DB3E +@SimSeq_13/221/1 +TACCTGCTTTTATCCTTTTACTATCTCTACTTCATATAACCGCCCACCCGGTCGGTTTTAAAAAAATGATAATTAAACTAGCATTTTTCACCAGCCTCCC ++ +G=DG;DGE<5A>GEGGDAEDFEGCBEEFGF?GF=CFCGAF=CFEEAG>@FFG?=GGEF7FCGE#A@B?E=FB@AB,C5AD@G,@BB@)F1D#F##GB#C# +@SimSeq_14/154/1 +CACTCAATATTTAACCGAGAGAGATCCGCGCAGGAACTGCTAATTCCTAGCCCCCAGGACTAACAGCCTGGCCCTCTCGTCGTGGGCTCGGTAAGTGAAG ++ +GDEBAFE=GBDG=G@GGGE?DE?FCGGDEG:GAC??BGDBEF=BGC:?FEEFAFB?-#CDFCDECEGA=E;FCEBEG#BEGDGDCEDAA###E5;:###E +@SimSeq_15/76/1 +GGTTGTTAGTCAAGGTTCTGGGTTTAGTTGTGGCATGGTCACTAAGGGGGAGGGGGTAATACTAGTCCCCTAATCCAGATCGGAAGAGCGGTTCAGCAGG ++ +GEGDGGEDA=GFG@=FGGGG=GDBFGGF@EAEGEEG:GFDGDBGCGDEEEDDGGG7CF@:@A=AA1@A?EAEECFEEDDCFDBEDEDBD###F>=BA@E# +@SimSeq_16/55/1 +TTTATGGTTCAGGCACGGATCCTAAGTCATTGGTGGTAGTCCAAACATTGATATTAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAAAAAAAA ++ +ADGFBFCCF?DGDEGDFDDFDGEGGE@CDFD=DEBFC-G:EGBF?B+EGBDAGEAE?DEFCBF'?=@E5E=FGBE;=AFECA15FDBA#EE,:5B=#DEB +@SimSeq_17/122/1 +CCCCAATAACAGGCCTCATCTCCTGAACAGACACCCTACTATGACTATCAATTCTACTAGCTTTCCGAGTAAAAATCCCCCTCTATGGTTTACATCTATG ++ +GGDGCEGGFGGDEGGCGCGDAGC7GFBDAC=GCEDD;AEGDG?2A>E=G*CE>D:GDG:EG?D->A#>DE1BBE2DEBDCDBEF8?F6??D#C:CE##FE +@SimSeq_18/188/1 +TTTGTAGTTGTATTCGGTAGTGGTTTGTTAATTATTGAGGGTGGAAGTTTTATGCCTTTAGTGGTGTTATTAGTTTATTTAGGGGGGTTATTGGTGGTGT ++ +FG=DFDFGGGGFGGE=DGD=BGDEGEFG;FB=C=EEAFGCEA5EBDGEGBDF?AEAEAFC>BAE:#EG=BDG##E=GG@#CEFA;?#DCE@-#BC6D### +@SimSeq_19/46/1 +ANACCACAAACGCCAATATGCGTAACAAACCCCAGCAAAGAACCCCAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAAAAAAAAAAAAAAAAA ++ +D#DGFEF>GDG3GEF?EEGCGFGGGGD?EGGG:GGGDGG:EECFFG-3ECDD@E5>?#F@@DA#F8@:;:GBG9CEE9C>DEDB7DCE:#?:EEF#55A# +@SimSeq_1a/16/1 +GTGATATAAAGGAGGTAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA ++ +DAGG>GGGAECFDEFBFDBE?GBDEFGCGGGE5CBCGBGEB?DDAG?A=BGA#0=E?GBDFF#CG>E??A=?;C=CAE=?#DE;?#DF>5EBF?B#E##< +@SimSeq_1b/116/1 +TGGAGGATACAGATGAAAATGCTAGAGTATCGCTTGATGAAAAGTGCATTATTAAGAAGAGTCTTGTTAGAATCTGAATTAATAGGGTTAGGCCTAGTAG ++ +7B9BG;6GDGGGFFGBGD==DGGGAEGEBGDG@@FFDFEGFE?;?EBA#?>FED4AB:DD?DE0B0F@B@FEE;EE?E??C:F5EA#=9ABE7C#GB-## +@SimSeq_1c/138/1 +TTGTGTTTGCTTTTTGTGTTGGGTTTACTGATGATCCATTTGGGGGGTTTTGAGGAGTGGGGGTGTCTAAGTGGGTCGTATTTTTTTGATGGGCGGGGTT ++ +E<GFCDGGFDG2@DGE;FGFFEE,:GGGDGGDGBGEFD?GG==@EFE??EA>ECFDED@?EDFB??FEBFAF>A?,:DGA:#,FC#G;#C??A##:E>#D +@SimSeq_1d/195/1 +TTGGGCAGTCAGTAGGTTTAGGGCGACTACGATTGAAACGGTGATTGAGGATATTGTGAGTATGATAAATAGGTTAATTGCTTTTCCACGGGTGGGATAA ++ +FC5GFGGCDG:EG6EFED=?:FCD@GGEDBF>DGEFDFDDFE?3AACCGCEF?DDDGADBE7CADB?BBB@B???E#BF@>#GFE:C#FAD@B#BDB#C# +@SimSeq_1e/0/1 +AGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAA ++ +AEEGD=GFBFG=FADFGDEGGEB8@CBGFEFBGGGEGAGAFGDD=DAFFBEDEBDF7EBGA?GGB@?EBA#DEF@D.B<ECDAC@B??###F-##@2#D? +@SimSeq_1f/145/1 +ACTGCCCTACACTTCCTCCTTCCATTCGCCCTATTGGCTTCACTCATCACCCACCTGATCTTCCTCCATGAACGAGGATCATTTAACCCCCTAGGAATTA ++ +?GE?6AGFDDF8A?GDGFDEDBBEEF;EEDCF?BFFDD7#EGGADF>GDBDDDFG:CFGC7C?DEG@E6@GBABF<3:EBE#EAC=#BCA##B#CG;##A +@SimSeq_20/104/1 +TTAGCATTGTAGCAGGTTTAGGTTTTTGAGGCTCGCCGTATTATGAGTTCGAGCAGAGGCGACTAATAAGGCAAGGCCAACTCCTGCTTGACAAGCGGAT ++ +DGCEBCD-GGGECEEF?DACFEEADGDGAG7AEFFGC?EDECG0A?:#-DG7GGDEE>??BFC==*GE@:>=AD#C@@F?GA;@CAE?A##BAFEB##:C +@SimSeq_21/50/1 +AGAAACTTATGGTTAGTAGGAGAGCCTTGCTTATTAGGGCAATGGTGAATAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAAAAAAAAAAAAA ++ +EE/DDGGFFEG=GG=EBCE9EB:EEABBD?FD;G::FC@#GE?GEG?D=GCA?FAECGE6AAE-D?A0DF:5>C<A#EACFBE#DDDD#D>E5>#DEB## +@SimSeq_22/0/1 +AGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA ++ +@GFGGDFDGGFD?EEGGGGBCEDDCCDFF@GB;EEGGG7DD5EGFAG6EDGEAFGAABCG.B:EBEGE:F5@-D?6C2=F:#GCC#6@5=E?=D=E#A## +@SimSeq_23/196/1 +TAAATTTTACCTTCTTCCCACAACACTTCCTAGGACTATCTGGGATACCTCGACGATACTCGGACTACCCAGACGCATACACCCTCTGAAACCGAACATC ++ +EEDG@CGGFGDGFDDAGGFDGDCGFDCAGE<AGGEGDGBG@G4BDEE-?EDG?E9C>E@?CDGG,BEBGFGC=#A@C>D#ADDG5C9E#,#BA#56@=:A +@SimSeq_24/0/1 +ATATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA ++ +F3GFGG;:CCGGGDAGE@AGD?AC>0E@FE@DFG=GBEDFFGEGFGGEEE#FBF@G3GEEDB#B:DE?A><5:D=EB=BFGDAG@GEBAG#5#ADDF#D5 +@SimSeq_25/224/1 +CTAAACAGAATAATATAGAAGATGTTATTCCATGGGCAATCATTAGGATTATTGACCCTATCGGGGTTAGTTGGTTTCGGGTGAGAATTGCAGCTGTTAC ++ +G?EGDEGGFDFDFFEGFDABFGBCF5G=G>FF<F5DD7BBGG?>FG<F?EFBEEAFDE#EGGCECDC)#DG#E@5D?E7F>#DE:>#DF##=BB?=#G@# +@SimSeq_26/97/1 +GATAACTGCAACCATGGAAATTAAGGATCCAATTGATGATGTTAGGTTTCAGAGGGTGTATGCGTCTGGGTAGTCCGAGTATCGTCGAGGTATCCCAAGA ++ +>GGAGFGBEDFGGGDFGFGA*GDFD=DGDEDCGG>FFEE5E=DDGCCCE?GDE3=CGDFGGC?>#DEFCE8DFEED#BFCBD?DA#D#A#AA#FB#9#?E +@SimSeq_27/144/1 +ATAGGTGATTAAAGAAGCTAATAATTTTTAGGTCTGAATCTATATATAATTCGGTGAATTCTATAATTGATCATGTGACAAACAGTGCTGTGGGGACGAA ++ +E;GGA?GDD?GEGFGDEGAGGAB:CE@FE:BEFEFFF8GGDGG;?G@D=G?G??6AF#AD#FB??=#=EF3;#E@E>:DB?B?;F#D#?:C#E#E??C#? +@SimSeq_28/124/1 +TAATTATGGAATTTGCTGGGGTGAAGTTTTCAGGGTCCCCTAATAGGGCTGGTAGGTAGAGAGCTAAGATGAGTAGTGAGGAGGCAGCTAGTGCTGCTCC ++ +D9AFG:D=GGGCG@AGGBGFCEGD?GEEEGFE@E?ECBGEECFDBFEF;DFE:AB@4?FEEEAFGAE::>5DCEEEA:D?C@#G@GCE#BCAD;E8=?D# +@SimSeq_29/224/1 +TGATGAGTGAAGCCAATAGGGCGAATGGAAGGAGGAAGTGTAGGGCAGTGAAGCGTGTAAGTGTTGCGTTGTCAACAGAGGGGCCGCCTCAAATTCATGG ++ +EGGGCGEGDDCGGGGDACDA;GFEG:FCGGDEC=:GFF>BAEDFEEGF;CE>E@CE>GCB=CA#:C>F(A;B85B7CCE=BA6##A>>E@:5@B#BDE#B +@SimSeq_2a/120/1 +CGAGCGGAGATGGTATGGGGAGGGGGGACCACATGGCGAGAGCAAGTATTAAGGCAGCTGTTGGGGCCAGGATGAAAAGGGTAGGGGTGGCAAGCAGGGG ++ +E7GFGDEGB;CDGGFFDEDFAGEDEG>BFDFGE=BEGGCG>?FDBCFADGD#9AB@EEDGFBAG:1DD:@BGAB>5BD=E5#=@#CCB#A?##7=?E### +@SimSeq_2b/140/1 +AAGTTGCTTTTTCTGCGAGGAGTTGGTTGATTGTTAATCATTTTGGTATAAAGCCGGCTAGGGGTGGGAGGCCGGATAGTGATAGGAGGGAAATTATCGG ++ +GE-GFGGGGGEFFEF=CG=FACEEEDFGE7EG@BC#EGDBAEA?FGCEC>4-C;G>ED@#9E@DG:DD@>CE??CEA:65@C#E##5AE)G<AE##:##= +@SimSeq_2c/0/1 +AGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAA ++ +DGF=D>DCGGGGDDFGG??GFCGGEE??FEB=DFG??EGDF>DDDA?.GFEEG3AEDC@GEC=?C:EE=?BC?F#1ED.DE=D695EGBFE;.#E;BA#> +@SimSeq_2d/200/1 +CAAGCCGTAGTTGCTGCTTGACTGCTATAGACTACGTGGGAGGTTCGGATTAGCAGGAAAATGCCTGCTACTACTATGGTGCTTGAGTGGAGTAGAGCTG ++ +G?F=EGBEEDDDGFF=GADDEGGFGEB=G9BGFDGCGFBDFG?BG=?DC=3:AGDG#.BD<DCBGG>9CEE@F>6EF>ADAFFC5D@F@ABCB>##<A:G +@SimSeq_2e/53/1 +CTCGCTGCTATATCATGAATGATCCTAAACAACATCAGTCTGGACATCCAAGAAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAAAAAAAAAA ++ +<GGG@GFABBDFGGFDGFFEEEEEG5ED=FBGDEDEGCG=?GD5EGD?G:ACF??@BAC:EFGF>F4>##CB5B:#2A?=;?=3B6C;@@#AD:>@5#B# +@SimSeq_2f/212/1 +ACTATCTGGGATACCTCGACGATACTCGGACTACCCAGACGCATACACCCTCTGAAACCTAACATCATCAATTGGATCCTTAATTTCCATGGTTGCAGTT ++ +G6DEEGGDFDD98GEGFF5?AFEF?CGDEGCB=EFFACCAAGGADFGEE1CFD=FFGEGBGDDC@:G#G:#BE##7#DG#CAD#?DC#G@@A6>B#BFC# +@SimSeq_30/65/1 +GCGCTGCGAGCTTGGCTGTGTTCGATATGGTTAGTTGGGGGGTGTTGGATGGTAATATAGAGGATAGATCGGAAGAGCGGTTCGGCAGGAATGCCGAGAC ++ +G)DGGEBEGGGGGGDEEEBGBGFGAECE7GEG'EFGG:5AAA>C5FD:?FD<AGFEG:EF=GEFF4=ECD@#AFA;#GDA#?6#DD?>DB>EE?=#F?## +@SimSeq_31/205/1 +ATTTCTTTTAGCCTAGAGAGTATTCTTGTTTAGTAATTGCTTTCATTAGTGTGGTTGATAGTAGGAGAGTGGTGGTCGCTATGGTGAGGAGGTTGATGGT ++ +DFFFFGEFDEA?CGFFGEF;EGFGGDEFAEEGDEG=FE9G=EGGEGF9=CCG=G?DF#ECEFD?CEE@35B#CG?AG=B5F<CE>=9#C:#F#>DFAAA# +@SimSeq_32/111/1 +TTCACTGTATTCTACATTAAATCCGGATACTAGTTCCGATTCTCCTTCTGTTAAGTCAAAGGGGGCGCGGTTTGTTTCTGCTAGTGTGGAAGTCTACCAT ++ +D7GGD@GGGFCFECGGGEBEE-GDACDBCF1EDGGGE<5G:E=?F)A?AGEED@BEABB=>8B==5:>C=,?@?E:BE-D2F?C6BDDF6F?C#:#BG?# +@SimSeq_33/123/1 +GTCCCCTCTGTTGACAACGCAACACTTACACGCTTCACTGCCCTACACTTCCTCCTTCCATTCGCCCTATTGGCTTCACTCATCACCCACCTGATCTTCC ++ +B8EGCDFDGFBEEGCEGBEDE>?EGBEFFDGF45?FAGA?G,G?G-DEECEF>=ECEDECGAD#GAEFF?5EDC;?=GC>?ABFB?E9#CF*#5D##G#B +@SimSeq_34/23/1 +TAATAGGGTTAGGCCTAGTAGTGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAAAAAT ++ +FEF3DDGEFEFDFDGDBDFCGFGBFEGDGGADFCDEEAGDD9GGGBEGB>CCFCEEG,BFG5GD#5BEDCFDADE=AD##G#FBF5AE@)E9B###-### +@SimSeq_35/203/1 +TCGTAGTCGCCCTAAACCTACTGACTGCCAAAACATCACCCGACCCAGAAAAATTATCCCCTTACGAATGTGGATTCGACCCCCTCGGCTCTGCTCGCTT ++ +F-,F6DFGDF7GEFGDF?D;GCEGCGBGG7B==D=G:FGGDE<GFFBAFEG?E?EEEFDBCAF#EGABEEAC<=*>G#D,DCFEEE##=A;=DEECCD## +@SimSeq_36/0/1 +AGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAATAAAAAAA ++ +FG:FGFEEGEGGGG??EGEFDEFDD?GFGF=G>GGFEEFFE-EBGEED5E<EAEEEEDG?E5FB8D5=B9=EA4DE#CC@DC#:#=>AA2?G#B###BE# +@SimSeq_37/282/1 +CTTCTGAGTACCAGAAGTTCTCCAAGGAATATCCACAACTGCCGCAAGCTTCCTATTAACCTGACAAAAACTAGGACCATTAATTATACTATTCCTAATT ++ +FD=E?F=G9GFGGFEEG=BC=GEEBDDAGBFDFG=GGDGCDABECGC*GCGG#B>D:CGDF>#EE5G4DBBC94CCE8FE?*#DAFEFC5/D<@#5BB## +@SimSeq_38/212/1 +GCCAGCGCTTTTCCTTTAGCCACGGGAGTGTGGTGAGGTTTGGTCTTGTTGGGATGGAGGGACTTAGGGGTAACATAGGTGGGTTCAACTATTTTGTTCT ++ +DGGEFA:GCGE:DGBGEDAFGEGE@?GDEG+>;BCEDFE>EGEBGDGACCEFGBF=6?@DBCFF=#EDCE1F::<62D>#2#?6EEE?EEA?AC#BB?DC +@SimSeq_39/149/1 +AGACCAAACCTCACCACCCTCCCGTGGCTAAAGGAAAAGCGCTGGCCTTGTAAGACCGAAGTGGACGGAACCAACCCTCCCGTGAGTACACCAGCACCCT ++ +FGEBEE==G?:GG>FGCFEDEFACGEDEEADFF5FE)D5F;GFDFEF,EGEBG@EE,AADGGE5B>GF:DEC::E?EC5G##36BGD@#7=?#@)#,+ED +@SimSeq_3a/195/1 +ATCGGAACTAGTATCCGGATTTAATGTAGAATACAGTGCAAGCCCTTTCGCACTTTTTTTCCTAGCTGAATATGCCAACATTAGATTAATAAATACACTC ++ +FCGG?D-DEFFCEG?EDEGG??FGFE>DGDGEDFBFG4:EEEGGGGGDGGGFEBECDDEF%DA9?GAGF=CB#:13?BEA;C#-ED=A##CC:#=D#A#1 +@SimSeq_3b/93/1 +TCCCTCGTATTTTACATAATCTTCGCCCTATTATTCCCTAAACTATACTACCCAAACACCTCAGACGTTCAAGAAGTAGAAGTAATCTGAACCAGATCGG ++ +FFF,FGGGFDE?FDGDAGCDAGCF1D?DCDGFEF;B8GBDBG?DBG=?FEEAGFC5:F'D?FBEBE.?:D-#EC??@?#=#GDD#:C?E?D?#A:#D#A# +@SimSeq_3c/122/1 +TTGATAGTTTTTGGCTCATTCGCAGGGTTGTGGTTGGGAAGAAGTGGTGGAATAGGTGGTTGAAGTAGGTTAGTTTAGTTAGGAAGGGCGGTTGGGTGTC ++ +GGGG@AD>CD<?:G?GGGEAGFGGG@FBE:GGBBED=GFEEGA>E?AD<@E>=CF?5#D?DF@B?#B>:='C?B#;EE:G#>B9AE#5AA;?EF5##@1C
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/input2.fq Thu Feb 15 14:23:13 2024 +0000 @@ -0,0 +1,240 @@ +@SimSeq_1/229/2 +GGACTAGTATTACCCCCTCCCCCTTAGTGACCATGCCAAAACTAAACCCAGAACCTTGACTAACAACCTTCCTAATCGTTTGAATCTCCCTTATTGTTAT ++ +BECAFFGCG#5AFGEBB5EBD@ED:=FF?=:GGCD:G?-:AEECECEGABF57F-FFEAFF#EEEAC=A5CEDDDCA#-A#@C=#@?##E#A=@E#>#BD +@SimSeq_2/49/2 +ATTTAGGGCTTTGAAGGCCCTCGGCTTGGAATTTAAACCTAATTTCTTTAGATCGGAAGAGCGTCGTGTAGGGACGGAGTGTAAAAAAAAAAAAAAAAAA ++ +=AFGG2=CFE6FG:?GEGGADBECGFEEFC>BB96E>5>DDB55=D?:-A=CDFGC4DDCAFA?G#C=#FCD8?#6AFF,EDA#BA##5;D6C#/#-A-A +@SimSeq_3/149/2 +GGTTGTTGATATTTTGTCTAGTGTTAACAGTGTAGCGGAGATGGTGGTGGAGTAGATGTAGAAGGTTAGGATGGCTAGTGATGGTGGGTATTTAATAACT ++ +GFGEBB=EB@FCDEDGFC#FGD5GGCGGF<BBFFAG?:ECD@BCE:BD9@??B-DADGG7DABADED###:#ABC#ECB?B#F#DF#E##69@8##-#,B +@SimSeq_4/197/2 +TTACAAATTGGATTTTAGTTCATGTTGGGTGAAGGGTAAATCCTGTAAAGAGTGGGAATCAGTGGGTGAATCCGCTCATGATAGCGAAGACTGCTCCTAT ++ +DFGGGDGF6EGDG;BFB?EEGGG>=E:GBGDGB=G.C?GEDCF#E:E5CB#DBGFDE>CFFC=F7D?8ED=DDCE#9BC@B;#B8#A2;#AECE??C@#E +@SimSeq_5/40/2 +GCCGCAATACCACCATGCTCCATCCTCCTCTGAGAGGGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTATAAAAAAAAAAAAAAAAAAAAAAAAAAA ++ +GBCEGF:CE0D5FGED7FCAFCBFCG;EAFA#A##FDDCG:8GFADCBCAEDFC#EDFGEDCEDGFDC;D?-D?B?FA7AD#C5#7CA#>###?A;;### +@SimSeq_6/190/2 +GCTAAAAGAAATTAGGTTTAAATTCCAAGCCGAGGGCCTTCAAAGACCTAAATGGGAGTAAACAACCCCCCATTTCTTGATTTAAGGTTTGCAGGACTCT ++ +GDF=E:EEB=;A>EAEGE@FCGGAGFDEGEGE#:DDGF?BC/DEC#F??=GDD#FDE9@1FGE?EFCDGAGA#EDAB=7FED59=AE+#9##C57>A#B# +@SimSeq_7/178/2 +ATAGGAAGTACCATTCGGGTTTGATGTGTGTTGGGGTAATTATGGAATTTGCTGGGGTGAAGTTTTCAGGGTCCCCTAATAGGGCTGGTAGGTAGAGGGC ++ +DDEGGGG?D>G@?:GEF>GCGEG=FE;D@GFFE=EC5>=A:GAB?GA?E@D?E7?BDDG:GEA#CGB@;7AEB>;DBCC#CEA2B@:CB#>C##BC###@ +@SimSeq_8/18/2 +ATAAGGGGAGGGAGGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAAAAAAAAAAAAAAACAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAA ++ +AFDG2D?EG?E;?FDG4EFGGD:EEABFEFG??GDEE3G0#DEFE?CDGBGCCFFF,#GBBEG:?C#AF;?GG?E5C>#AGD-3###4#E#A#>#DB?/: +@SimSeq_9/103/2 +CCAGCCATTGTCCTTATCTCAATTGCCCTACCATCACTACGCACCCTATACCTCATAGACGAAACCAATAACCCCTGCCTGACTATTAAAGTAAGCGGAC ++ +FEG?GGAG:GEAGEF;GDGEG?:EAGB#EAEEG?>#EC57C:F:4FGC-#<EFEAGGD#EEE8GGGFCA-9A:AG5FD'DAGB5A#@G4AEB#CA<#>#: +@SimSeq_a/334/2 +CCAATGGATGGGATTGCCCATGAGTGTAGTACATCTGCTGCTGTAATTAGTACTCGGATGGTTGAGTTAGTTGGGGTGATTAGGCGGTGGTCAACTTCGA ++ +F:CBBDG9?@AGDE<FEEG?GCAD#EDE5FF5FF#G&BDEGDGD>?C:>CCFF;FADGGEDC??B-CEBBDBBC#:#0DDB##+E#?5#AFB:D?##### +@SimSeq_b/247/2 +TCTGAGATCTGCGGGGCAAACCACAGCTTCATACCTATCACCATAGAAACCATCCCACTAAACCACTTCCAACTCTGATTAGAATATTCTATTCTCTCCT ++ +DGGGED6BGDGGB=FFAEDGFDGEG056AGGFECECGDG8FD:DFFG=FDBD-BDEB>E>AF;##GCC?GB5CDG7:E#A#;#A#@?@C###GA?G#?D# +@SimSeq_c/0/2 +ATATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA ++ +FFGFG=G:GE=C86GFB:D?GGF?=E-AGG:DE;EG*EEF?G.DGBCD6CGFCD2#=GECE=?F;AEE#5;GD5>8DE7#A;E,=5D5@###C#5#FF#C +@SimSeq_d/127/2 +TAATTAAACTAGCATTTTTCACAAGCCTCCCTCCCCTTATTTTATTAACTTACAACAACATAGTTGCCCTCTCATTCACTTGACATTGACTCTGCATAGG ++ +DBGFFBABGGDGEEAEE?FBADGC:DFGBCE?BEDAD@CF7EEBC=>A?F5:-5EBEBFDCGGE;#?:?CB=G=3'AD<::7AD?E7E:=*C###FDE## +@SimSeq_e/84/2 +GGGAGCGGTTGATGAGTTTAATGATTGGGTGGGATTTTCGTAGTTGGTGGGTCATGGGTTTTTATAGTTGAAATACAACAGGGGAGATCGGAAGAGCGTC ++ +CEFEEEGEFFDGEGE9GB,AFDE?C>@DDEDFGAFGDGGBED:G)E4E?C>D(EEF#F=GC9D#E>===ECD;###:GC#F<C#?<B######B###=B# +@SimSeq_f/64/2 +ACAGGCGTTAATATAAGAGGTGTTTAGTGCTTCAATAATAGCGTCTTTGGAAAAGAATCCTGAGAGATCGTAAGAGCGTCGTGTAGGGAAGGAGCGTAAA ++ +;GDGEGEGC9BGGEGEEGE-ADDAGEFCG=A9A;G;FFFDEE<AFABEFCG@:B=@7=EFC:A??DEGGD#:>=CAGE##EABF>##EBC##A4##C4;> +@SimSeq_10/154/2 +CACTAGACCCCCAATTATCCCACCAGGGGAAAAGCAATTAACCTATTTATCATACTCACAATATCCTCAATCACCGTTTCAATCGTAGTCGCCCTAAAGC ++ +DFGAFDGGE9=?;:BB>B>:BCCB?DEFD-G?FB@=E9GG:E;E5B)==DEC-GDG9GAG6F;CE:57#A?::DE,EB>BF<ABC=##;B#DC##?##+C +@SimSeq_11/66/2 +CTATTCACCATTGCCCTAATAAGCAAGGCTCTCCTACTAACCATAAGTTTCTTATGGGTTCGAGCAAGATCGGACGAGCGTCGTGTAGGGAAAGCGTTTA ++ +EEGAEFGFF*GGB;D;GA6B@GFCCEF?EDECGG4EB7?EED#EAAA?<B>2DBC=FA=@BDC9E=BG=E:DCB#GDEA?CD?##C#?5##E#:#7F#@= +@SimSeq_12/241/2 +ACACAACCCGCCTCTGACGAAAATCTACCCCCCAGAGTAACTTTACCATCAACCTCCTCACCATAGCGACCACCACTCTCCGACTATCAACGACACTACT ++ +FA=DEDGAGGGG=GCFGGGEDF:CG3GCEF@GBGDAG?G6=FEF=GBG?G5@#GEABDDD-@E;#GA9AF=A#F#?AE#:E*?BED8?DE#?#D=##A#C +@SimSeq_13/221/2 +GAAGAAGATTGAGAAGGTGTCGAGTTTAAGGCTTAAGTAGATGGGGCAGGTTCCTATGCTGAGTCAATGTCAAGTGAATGAGAGGGGAACTATGGTGTTG ++ +FBGGEGGEGDCAEADD?EE=EFGBEGGFFE@G?EGEDGEFGC3BDECBB#CD?FED=D55#F#FA6@<@BFDD@;ECB:?#D:###C#5A###=#@##A# +@SimSeq_14/154/2 +GTAAGGGGAAGTAGTCTCCTATTTTTAGGTTTACATTCTAATGTTTTGTTTAAACTATACTTACCGAGCCCACGACGAGAGGGCCAGGCTGGTAGTCCTG ++ +#<GGAD#DFGA>=GFGGGGAEGB5DEGEDE*6CDE'EADGG?CAGDF=D54G-<CDDCFECEFGGDA#EE#6=FGEG##-D#5G##A#>FA#B5###B?# +@SimSeq_15/76/2 +GGATTAGGGGACTAGTATTACCCCCTCCCCCTTAGTGACCATGCCACAACTAAACCCAGAACCTTGACTAACAACCAGATCGGAACTGCGTCGTGTAGGC ++ +DC?D@EGFDGGGCAGDAAGAD;DFGBABDBCDB>FCFFDAGB;FEB=F#,DFD5CF-ECEFF>?G#C#@GGEEGD#-#B?D?;<-#1BG7?59####D?# +@SimSeq_16/55/2 +AATATCAATGTTTGGACTACCACCAATGACTTAGGATCCGTGCCTGAACCATAAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAAAAAAAAACAA ++ +EFGGGGEC:GFGFG-BGAFDAGG=G>C9E@GEGAEABCECAAD76CFDAC:FC?5GE??FC=EBCG#ADDG=:FAFE=BBAB6A0;#D#B:F:#><D=#A +@SimSeq_17/122/2 +TTCTACATGAGCCTTGGGGAGTCATAGATGTAAACCATAGAGGGGGAGTTTTACTAGGAAAGCTAGTAGAATTGATAGCCATAGTAGGGTGTCTGTTCAG ++ +GGFEG3GE>DEBG;GDGCEGDFE=C?CG:;GABEAG?#BF<7=FG5.-9>DA?=AE<7FE###?=#EED:=GF#EGF##2>C:F4F?EE=B#E=#CB### +@SimSeq_18/188/2 +GCCCATCAAAAAAATACGACCCACTTAGACACCCCCACTCCTCAAAACCCCCCAAATGGATCATCAGTAAACCCAACACAAAAAGCAAACACCACCAAGA ++ +FGG2CDGC5GGGGEGF6?DGGG:8EEFE>4CBFFDE>3BFD=E=?B>@DC?E4;G(/F#@>G;:04D#E>:A>E=?#FFBDBB?-#8D#AE;#?#=###C +@SimSeq_19/46/2 +GTGGTTCTTTGCTGGGGTTTGTTACGCATATTGGCGTTTGTGGTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAAAAAAAAAAAAAAAAAAAAA ++ +>?G4GFD-B7EGGG=D>(=#FFG>E9BGDEFG?DCDEGCGECCD67@7EFEE-FE#?ECAA;:A9GFA#CAF:EA#?DCEAG55#?#.#BC#F5EC4@## +@SimSeq_1a/16/2 +ACCTCCTTTATATCACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACA ++ +@GEAEGECGFAGFG:1FAG=GF@FEBG:C5FGDEEBGFGB#?EEFDG5GCF-=GEC#*@GD75DD@BD?;2A7;G@-G#?GF5F?#BB#F#A7EG##B#? +@SimSeq_1b/116/2 +ATTAAACTTTGGATCACTACTAGGCCTAACCCTATTAATTCAGATTCTAACAGGATTCTTCTTAATAATGCACTTTTCCTCAAGCGATACTCTACCATTT ++ +;E2GFG;;FFGG?:EG<BGE;#C>D-6EEA/7GDD=EA=GDG4E=FF?=?EE:?7GBEE@DG:-E#GA=55>:?E:F5#<>):*#?C#=<#=>B##:5?: +@SimSeq_1c/138/2 +ACCACAAACGCCAATATGCGTAACAAACCCCAGCAAAGAACCCCGCCCATCAAAAAAATACGACCCACTTAGACACCCCCACTACTCAAAACCCCCTAAA ++ +AGGAFGDGGGGFG;EDGEGCCGDGA@E;=GGEGFB-G=G=DFG:GGE?A:@E:DEDDBD5G#AGF:GD:EBG#F@E>G1F#EF#F#A:>##>A#B#>4## +@SimSeq_1d/195/2 +TCGTCTGACTTTTCCTCTATATCTCGATCTACTGATGAGGATCGTGCTTTTCTAGTACAAACAATACAAGTGACTTCCAATCACTAGACCCCCAATTATC ++ +F?D8GGGEGFDFGDEF=FE.=EFCEEABC+?FGE=C?DFC'.GBF=GG7F*B#:>BEA5??A?:5#EC==#.2G?DA.A?#EF#1AC#B##=F?EA###A +@SimSeq_1e/0/2 +AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAAAAAAAAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA ++ +GGGG0DFGGGAEC=<E?DGAEGFAAED?=GBFFDBDDC-3G<F-=55<:ECF=5;D>@#AEDEB?DAED:D:-#DDE#AE:A:=E+8#DA-#.E@#?#8# +@SimSeq_1f/145/2 +TTATGGTGAAGTAGGGGTGGAATGGGATTTTGTCAGCATTTGGGCTAATTCCTAGGGGGTTAAATGATCCTCGTTCAGGGAGGAAGATCAGGTGGGTGCT ++ +GAED=EGGEFGG<1D=6FAGE;GED)?DGG#EGE<B:GCAEGEE:GB#=E;C5G?#ADBCB@D#BDA-AG5CE:GEF#?EB#/>#####DGC#E+##:#A +@SimSeq_20/104/2 +CCCTATCCGCTTGTGAAGCAGGAGTTGGCCTTGCCTTATTAGTCGCCTCTGCTCGAACTCATAATACGGCGAGCCTCACAAACCTACACCTGCTACCATG ++ +7FBGGGGFG@E5>GGGDEG5FAG>:DA5GFG?EAE;:GE>E>?F@CG6.D=B,A;#G#AG8A?CDADACCC?C;A#DD.AFBCAB##B-A#DF3#A##?? +@SimSeq_21/50/2 +ATTCACCATTGCCCTAATAAGCAAGGCTCTCCTACTAACCATAAGTTTCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAAAAAAAAAAAAAAAAC ++ +GD==GF=GGGADEEFG=D>@BGEBEC:E>D:GAFG#=GCGAF5FFG56G'=G?A=BE@DC#GCEFG?FG>>A:B@>.9#F2#AFFG##ECEB1-#?##3# +@SimSeq_22/0/2 +AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAAAA ++ +FFAFGCEF:EDDDD:<>CF?:B?FG:EC=<AAD#BF:FDG:5EE@ECECF1DF9BE?DE?GE5C@FE>BAEC5?;-=C@#E@##:?##?<=D##B##### +@SimSeq_23/196/2 +TCGAGTGCTGTCACTTTTCGTTTTGATGTGAATGCTTCTCAGATAATAAATATTAGAAGGATAACTGCAACCATGGAAATTAAGGATCCAATTGATGATG ++ +@GCEDDGFGEE?BGFG?DGADDGG<G#GBDB=>:E?BD:GFGGGDEEGAC#E5AEDEG-:AB5=EF>:2;?:=EDC#B-@CC?C@######-?#B?GE## +@SimSeq_24/0/2 +AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAAAAAAAAAAA ++ +FED=?GFBG>3ED=FDFFDGEGFAGC=:G@6FE5EF?EF@=AD#ADAEBFDE/FCE,?;DC-:CDACCD#DE>B5D4A#+.C+C###C>FC##A####D? +@SimSeq_25/224/2 +CACAATCTACCCCCCAATTTTAGGCCTAGCGCTCTGGGGGGCACTTATGACAAGCCTCATCTGCTTGCGACAAACGGACCTAAAATCCCTAATCGCTGAC ++ +DGD=GEB?EFGDEEGFEBGC<EFBGFGEAFDABDDGCG-D<G=5BGED:CCF:.DEGABFA:EF?D6.6GB75:FF.?>E)E-==:#5#C:A?/B#B#># +@SimSeq_26/97/2 +TGGGATACCTCGACGATACTCGGACTACCCAGACGCATACACCCTCTGAAACCTAACATCAGCAATTGGATCCTTACTTTCCATGGTTGCGGTTATCCGA ++ +GFF#@CFG=EGEFBCF5GCDGEEF?=EBAEGEECGGB,5EG#C:DF>FFCEFDCEDE:EGC&=@@*FB<BE7#>=<#:=4#8#=C@E#=##>###AC#FA +@SimSeq_27/144/2 +GCCCCATCTACTTAAGCCTTAAACTCGACACCTTCTCAATCTTCTTCGTCCCCACAGCACTGTTTGTCACATGATCAATTATAGAATTCACCGAATTAGA ++ +?GGAEGGGAEFGA;GDEGF;GCGDFG?E=D;FBDC8CBC=G:GEA)-?@DGAEAGDD#AG:EEDECEE=5B:#5,#6A=?DCF5E?=#=E#EA##:<##F +@SimSeq_28/124/2 +TACTCCACCATAAAAGACGCCCTAGGAGCAGCACTAGCTGCCTCCTCACTACTCATCTTAGCTCTCTACCTACCAGCCCTATTAGGGGACCCTGGAACCT ++ +-G6A:@F:?GEG?EDGF?FGAGG8DEEGG<GAAE4FADE9?F?D#GD4;:EGB5E?GGAG#E;DEE?@DDE5<B=C=C#BE#6D?C#=GG@F#G##A##B +@SimSeq_29/224/2 +TCTACTCCTAATAGCCACAGCATTTATAGGCTATGTTCTCCCATGAGGACAAATATCATTCTGAGGAGCAACCGTAATCACGAATCTACTTTCTGCCACA ++ +GGGGGC6,?@EFGDFF=G6DGBBGBDD-E#EBGFF#ECEGC35G@?DB=G@DGF@,BFAAGB5F-E=F:G=7DFBG=?A;ADA##DB:A#;###=E#C## +@SimSeq_2a/120/2 +TTACCAAAGAGTTAACCCTTCCCCTGCTTGCCACCCCTACCCTTTTCATCCTGGCCCCAACAGCTGCCTTAATACGTGCTCACGCCATGTGGTCCCCCCT ++ +GGGGGEEFGEACDF-DG:B=?G@DD?FG-DDGBDCFFC@E>B::D::AF/?A;GBB2E:FBAF=9#?F@F#E:>G#@E,##6:C#%:##E#D#E#?#E## +@SimSeq_2b/140/2 +CATTATTTCTTTTTCAAAATCCCCAATCACCACCACCATCCTGATAATTTCCCTCCTATCACTATCCGGCCTCCCACCCCTAGCCGGGTTTATACCAAAA ++ +GGGG=AFGFFDC?GGFGEGGCCF;=GCED=EEEG=DG?E#ECEGBEDE5?D9:EGF:D=:@GA0E*G2EGCBF@A@ECF?B##A:B5#C=DC#?DF;-## +@SimSeq_2c/0/2 +AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA ++ +FEEEGGGD>GEGGFGCGBE5DF5F7F>6GFG6D?FGGFEE@E>??FDA8=?ACE?;:,DGF?=FCBFA5?EBA:@E=#+:=#F65@C>#=###7E#:FA# +@SimSeq_2d/200/2 +ATCCCTGCCATTGGCCTCGTCCTAGCAGCAACTGGAAAATCGGCCCAATTCGGTTTCCACCCATGACTCCCGGCAGCAATAGAAGGCCAGACACCAGTCT ++ +GBG:ED:>=G?G:EEDFGDFFGDBA<EGBED?BE@E:@BFGGG;::9AFGG@=EEGEFGD#:4D2ADCDEEAAFEBGGEC?A==0CEE###A#AD###:# +@SimSeq_2e/53/2 +TCTTGGATGTCCAGACTGATGTTGTTTAGGATCATTCATGATATAGCAGCGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAAAAAAAAAAATA ++ +AFD;G5CBFDGG=F=DEAGFFFGDD6BE6E5E;GGG$B=EFGGFE4?F?G3?GE5GE;6BGG;ADE=:E#DGGG#D)9B@E?D<:#1##D@.##?7#=#D +@SimSeq_2f/212/2 +GATGAGATGGGGGGCAGTTGTTAAGTCACTCAATGTTGGTTATTGTTATTTCGAGTGCTGTCACTTTTCGTTTTGATGTGAATGCTTCTCAGATAATAAA ++ +G0G:GGFFEFCGGGF,GGF:>DE@?G56G2GE?>DDEBEEG=DEECECF<>CDGA6C6DFGFFE??F9:FC18AE?#E=A?##:#BCCDGE##G>B#B#@ +@SimSeq_30/65/2 +ATCCTCTATATTACCATCCAACACCCCCCAACTAACCATATCGAACACAGCCAAGCTCGCAGCCCAGATCGGCAGAGCGTCGTGTAGGGAAAGAGTGTAA ++ +EDFFGE?GGE:FEDF;=7GFFGBGGDE=FBGFGGB?GEEG4AG=G5>EB#.EFDB:ED:CE<E?=#GEDA:=+#>#:AGDGB-69##A@-F?EGBECE6; +@SimSeq_31/205/2 +CTATTCTTCTATCTCCGACTATGATACAACTCCTCATCAACTATGCCACCAAGCACTACCAACACAACCCGCCTCTGACGAAAATCTACCCCCCCAAGTA ++ +EDAE6GEFBG#EDDDEEGGEGA=FCCFEGAA=FDFA>EE5EA-EEG=FA?#>@C6CFEGA=?BEDF:8>DE/DE-:C#FBD>?BCED#DA-?AE##5C## +@SimSeq_32/111/2 +TCAATAACAATATGGTATACTTCCACACTAGCAGAAACAAACCGCGCCCCCTTTGACTTAACAGAAGGAGAATCGGAACTAGTATCCGGATTTAATGTAG ++ +GGFGBBF=C=7AGEA2FECG(BF>8D?(DFEE?:AECG=FAGACE:A7FGGE?>?'#C-FEF7DFEA?A#A6CAD@D#?#?=#:#DB5#CFG##E>#@#7 +@SimSeq_33/123/2 +GTTAAATGATCCCCGTTCATGGAGGAAGATCAGGTGGGTGATGAGTGAAGCCAATAGGGCGAATGGAAGGAGGAAGTGTAGGGCAGTGAAGCGTGTAAGT ++ +DDGFGEFG?G>GAFBAFF=?D:GFAAAF4GA?EEBEC=BGEFCFEAEFD@?E=GB=CDEG=#G=?#D-:?EF;#;@E*A5C@,56#C7?G?#D9####?# +@SimSeq_34/23/2 +CACTACTAGGCCTAACCCTATTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAAAAAAAAAAAAAAA ++ +?=EGF3EAGFFGFACEG=BFCGFADCFD7>EGA:EGBF@:>*CD:CC??-:EEDE:D=D:GF=D=EE#->C1BE1CG+:##D#:D)#E#C#*@C#?A### +@SimSeq_35/203/2 +GTTGAGGGTGTGGATGGCTCATGTGAGTGGTAGTAGGATGGCAATTTCAAGGTCAAAAAGCAGGAATAGGATGCCTACTAGAAAGAACCGGATTGATAGG ++ +DEAEEGA-CFDFEGFGFBDA>AFGC>;AGDE2:AEA>FADFE;E=#GE@FCD:C@A5GGGDFFACB5E:C?#FCF=;E5C&@FF9#:CF:###C>F#?#C +@SimSeq_36/0/2 +AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAAAAAAAAAAAAG ++ +DG5F::GBDGCE?DGGEGD9DGD=A6EDBC>:EGEF=ADCA8GAFF:?@DFE:?G5E>5-GD=B?F5E5?.D5-#G?:F,?F4#/#>FD#=-A#B#@### +@SimSeq_37/282/2 +TAGAAGGTTAGGATGGCTAGTGATGGTGCGTATTTAATAATTACGATAATTCATGCTATTTCGGCAATGGACGATAGTGCTATTAATTTTCGTACTTGAG ++ +EGEBGG?GBFDGGGACGF?F=6AACFC,GEG0EGDE#DGEFD:C=7D1E#DGD1FEGG=6GE#@@DCCCDCE@A2=##EF##C=A8E#A<#:##=#?#B# +@SimSeq_38/212/2 +ACATGAATCGGAGGCCAACCAGTAAACCCCCCATATATTTTAATTGGCCAAACTGCCTCCCTATTCTACTTCATCATCATCCTAATCCTCATACCAATAG ++ +GDDCEGB==E?DEDEEFAAEAA?EEGCA>FFD-5;D@EF::FC5AE6G?GC?E=AFFEDABEE5EDE#.EBACD#BBAD#F@-#F-###?##:##?EF15 +@SimSeq_39/149/2 +GGCTTTGGGGGCCGAAGGTGCAAAGTTTTGTTTGCTTTCTTGATACCGGAGGGTGCTGGTGTACTCACGGGAGGGTTGGTTGCGTCCACTGCTGTCTTAC ++ +GGEFGA:EFDFD=GBAACGDGGG=G5AD6ECG:G?GEA@CB?A:5EB?A>BEAA?-G:AD5A9=DDB?A=E5AE#FEA5A##EA:D@C??-:D#BA###C +@SimSeq_3a/195/2 +CTTATGGTTAGTAGGAGAGCCTTGCTTATTAGGGCAATGGTGAATAGTGCTGGGAGGAAGGTGGGGGTTGATGGGCTGAGACACAGGGTGGTGGTCAGTG ++ +G?GGGEGEGAGEGFG=-B8F?C=AEG>E8EA@E#GG=?FDG#D:=EBGE@EECCDEDFFB=;EFAB=:#>FAC##BCD##B#EDG#??G###@###GD># +@SimSeq_3b/93/2 +GGTTCAGATTACTTCTACTTCTTGAACGTCTGAGGTGTTTGGGTAGTATAGTTTAGGGAATAATAGGGCGAAGATTATGTAAAATACGAGGGAAGATCGG ++ +EFCFCDECEB=DDAGCE?GGACFGD:3EF?FED>F=AFCBDDEAFDG=GFA43=EF=BDEDGF?F#=EGC@B?#;-AC5=#AE6#?B#E#>#?:?####5 +@SimSeq_3c/122/2 +TAAATTCCCGCCCATTACCAACGACACCCAACCGCCCTTCCTAACTAAACTAACCTACTTCAACCCCCTATTCCACCACTTCTTGCCAACCACAACACTG ++ +-:E?DE>EG>BFFDDGGGBDEE=C:>DDG?FG8#8AA;5CAB?.F@B:AG==FA?C;D0-:=D##-BDEDEAC:F#:?C#GFGG-B>F#*#?C#E##>3#