Mercurial > repos > iuc > seurat_preprocessing
diff normalize_select_features_scale.xml @ 1:6bccf5f85f92 draft default tip
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/seurat_v5 commit 566984b588e88225f0b3f2dae88c6fd084315e7c
author | iuc |
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date | Tue, 05 Nov 2024 11:54:52 +0000 |
parents | c3170652bd98 |
children |
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--- a/normalize_select_features_scale.xml Wed Sep 11 10:20:38 2024 +0000 +++ b/normalize_select_features_scale.xml Tue Nov 05 11:54:52 2024 +0000 @@ -3,6 +3,7 @@ <macros> <import>macros.xml</import> </macros> + <expand macro="bio_tools"/> <expand macro="requirements"/> <expand macro="version_command"/> <command detect_errors="exit_code"><![CDATA[ @@ -404,7 +405,7 @@ Seurat ====== -Seurat is an R package designed for QC, analysis, and exploration of single-cell RNA-seq data. +Seurat is an R package designed for QC, analysis, and exploration of single-cell RNA-seq data. Seurat aims to enable users to identify and interpret sources of heterogeneity from single-cell transcriptomic measurements, and to integrate diverse types of single-cell data. @@ -444,7 +445,7 @@ Scale and regress the data with ScaleData ========================================= -Scale and center features in the dataset. +Scale and center features in the dataset. If variables are provided in vars.to.regress, they are individually regressed against each feature, and the resulting residuals are then scaled and centered. @@ -454,7 +455,7 @@ SCTransform =========== -Use this function as an alternative to the NormalizeData, FindVariableFeatures, ScaleData workflow. +Use this function as an alternative to the NormalizeData, FindVariableFeatures, ScaleData workflow. Results are saved in a new assay (named SCT by default) with counts being (corrected) counts, data being log1p(counts), scale.data being pearson residuals; sctransform::vst intermediate results are saved in misc slot of new assay.