Mercurial > repos > iuc > shasta
diff configs/Nanopore-UL-Jun2020.conf @ 0:60573349e9ae draft default tip
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/shasta commit 6d14992ea4fb1af09373d51b3a48166afcbd3a74"
author | iuc |
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date | Wed, 11 Nov 2020 21:54:51 +0000 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/configs/Nanopore-UL-Jun2020.conf Wed Nov 11 21:54:51 2020 +0000 @@ -0,0 +1,78 @@ +# DO NOT USE THIS FILE IF YOU HAVE READS CREATED BY A +# GUPPY VERSION OLDER THAN 3.6.0. + +# This file contains Shasta options that, as of June 2020, +# are known to work with Ultra-Long (UL) Oxford Nanopore reads +# under the following circumstances: + +# - Human genome assembly. +# - Coverage between 40x and 80x. If you have more coverage than that, +# you can use option "--Reads.minReadLength" to adjust coverage as desired. +# - Reads from Guppy 3.6.0 base caller. If you have reads +# from an older version of Guppy, use configuration file +# Nanopore-UL-Dec2019.conf instead. + +# To use this configuration file, specify Shasta option +# "--config AbsolutePathToThisFile". +# If you specify any conflicting values on the command line, +# the values specified on the command line take precedence. + +# In most cases, for best performance on a large assembly +# you will usually also want to use the following options, which +# cannot be specified in a configuration file: +# --memoryMode filesystem +# --memoryBacking 2M +# Using these options requires root access. + +# For detailed information on all available options see here: +# https://chanzuckerberg.github.io/shasta/CommandLineOptions.html + +# For information on running a small assembly for which +# performance is not essential see here: +# https://chanzuckerberg.github.io/shasta/QuickStart.html + +# For more information on running an assembly see here: +# https://chanzuckerberg.github.io/shasta/Running.html + +# For information on optimizing assembly performance see here: +# https://chanzuckerberg.github.io/shasta/Performance.html + + + +[Reads] +# If you have extra coverage, use this option to adjust coverage. +minReadLength = 50000 +noCache = True + +[Kmers] +# Due to the higher accuracy of Guppy 3.6.0 we use longer +# markers than usual. +k = 14 + +[MinHash] +minBucketSize = 10 +maxBucketSize = 30 +minFrequency = 5 + +[Align] +alignMethod = 3 +downsamplingFactor = 0.05 +matchScore = 6 +minAlignedFraction = 0.55 +minAlignedMarkerCount = 600 +sameChannelReadAlignment.suppressDeltaThreshold = 30 + +[ReadGraph] +maxAlignmentCount = 12 + + +[MarkerGraph] +simplifyMaxLength = 10,100,1000,10000,100000 +refineThreshold = 6 +crossEdgeCoverageThreshold = 3 + +[Assembly] +consensusCaller = Bayesian:guppy-3.6.0-a +detangleMethod = 1 + +