Mercurial > repos > iuc > snpfreqplot
annotate heatmap_for_variants.R @ 1:e362b3143cde draft
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1bde09fccd1a5412240ebd5c1f34a45ad73cebe2"
author | iuc |
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date | Thu, 10 Dec 2020 13:41:29 +0000 |
parents | 1062d6ad6503 |
children | dc51db22310c |
rev | line source |
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1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
iuc
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1 #!/usr/bin/env R |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
iuc
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2 |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
iuc
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3 suppressPackageStartupMessages(library(pheatmap)) |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
iuc
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4 suppressPackageStartupMessages(library(RColorBrewer)) |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
iuc
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5 suppressPackageStartupMessages(library(tidyverse)) |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
iuc
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6 |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
iuc
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7 fapply <- function(vect_ids, func) { |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
iuc
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8 #' List apply but preserve the names |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
iuc
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9 res <- lapply(vect_ids, func) |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
iuc
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10 names(res) <- vect_ids |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
iuc
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11 return(res) |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
iuc
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12 } |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
iuc
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13 |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
iuc
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14 # M A I N |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
iuc
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15 stopifnot(exists("samples")) |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
iuc
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16 variant_files <- fapply(samples$ids, read_and_process) # nolint |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
iuc
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17 |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
iuc
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18 extractall_data <- function(id) { |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
iuc
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19 variants <- variant_files[[id]] |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
iuc
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20 tmp <- variants %>% |
1
e362b3143cde
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1bde09fccd1a5412240ebd5c1f34a45ad73cebe2"
iuc
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21 mutate(unique_selectors = group_select) %>% |
e362b3143cde
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1bde09fccd1a5412240ebd5c1f34a45ad73cebe2"
iuc
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22 select(unique_selectors, AF) |
0
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
iuc
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23 colnames(tmp) <- c("Mutation", id) |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
iuc
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24 return(tmp) |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
iuc
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25 } |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
iuc
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26 |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
iuc
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27 extractall_annots <- function(id) { |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
iuc
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28 variants <- variant_files[[id]] |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
iuc
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29 tmp <- variants %>% |
1
e362b3143cde
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1bde09fccd1a5412240ebd5c1f34a45ad73cebe2"
iuc
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30 mutate(unique_selectors = group_select, |
0
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
iuc
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31 effect = EFF....EFFECT, gene = EFF....GENE) %>% |
1
e362b3143cde
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1bde09fccd1a5412240ebd5c1f34a45ad73cebe2"
iuc
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32 select(unique_selectors, effect, gene) |
e362b3143cde
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1bde09fccd1a5412240ebd5c1f34a45ad73cebe2"
iuc
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33 # allow "." as an alternative missing value in EFF.EFFECT and EFF.GENE |
e362b3143cde
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1bde09fccd1a5412240ebd5c1f34a45ad73cebe2"
iuc
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34 tmp$effect <- sub("^\\.$", "", tmp$effect) |
e362b3143cde
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1bde09fccd1a5412240ebd5c1f34a45ad73cebe2"
iuc
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35 tmp$gene <- sub("^\\.$", "", tmp$gene) |
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1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
iuc
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36 return(tmp) |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
iuc
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37 } |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
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38 |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
iuc
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39 # process allele frequencies |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
iuc
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40 processed_files <- fapply(samples$ids, extractall_data) |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
iuc
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41 final <- as_tibble( |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
iuc
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42 processed_files %>% |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
iuc
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43 reduce(full_join, by = "Mutation", copy = T)) |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
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44 |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
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45 final <- final[str_order(final$Mutation, numeric = T), ] %>% |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
iuc
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46 column_to_rownames("Mutation") ## sort and set rownames |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
iuc
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47 final[final < variant_frequency] <- NA ## adjust the variant frequency: |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
iuc
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48 final <- final[rowSums(is.na(final)) != ncol(final), ] |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
iuc
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49 final <- t(final) |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
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50 final[is.na(final)] <- 0 |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
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51 class(final) <- "numeric" |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
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52 |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
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53 # add annotations |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
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54 ## readout annotations |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
iuc
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55 processed_annots <- fapply(samples$ids, extractall_annots) |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
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56 ann_final <- processed_annots %>% |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
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57 reduce(function(x, y) { |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
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58 unique(rbind(x, y))}) %>% |
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e362b3143cde
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1bde09fccd1a5412240ebd5c1f34a45ad73cebe2"
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59 ## apply frequency filter |
e362b3143cde
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1bde09fccd1a5412240ebd5c1f34a45ad73cebe2"
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60 filter(unique_selectors %in% colnames(final)) |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
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61 ann_final <- as_tibble(ann_final[str_order( |
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e362b3143cde
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1bde09fccd1a5412240ebd5c1f34a45ad73cebe2"
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62 ann_final$unique_selectors, numeric = T), ]) %>% |
e362b3143cde
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1bde09fccd1a5412240ebd5c1f34a45ad73cebe2"
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63 column_to_rownames("unique_selectors") ## sort |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
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64 |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
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65 # rename annotations |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
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66 trans <- function(x, mapping, replace_missing=NULL) { |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
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67 # helper function for translating effects |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
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68 mapped <- mapping[[x]] |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
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69 if (is.null(mapped)) { |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
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70 if (is.null(replace_missing)) x else replace_missing |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
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71 } else { |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
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72 mapped |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
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73 } |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
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74 } |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
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75 |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
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76 # handle translation of classic SnpEff effects to sequence ontology terms |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
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77 # The following list defines the complete mapping between classic and So effect |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
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78 # terms even if not all of these are likely to appear in viral variant data. |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
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79 classic_snpeff_effects_to_so <- list( |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
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80 "coding_sequence_variant", "coding_sequence_variant", "disruptive_inframe_deletion", "disruptive_inframe_insertion", "inframe_deletion", "inframe_insertion", "downstream_gene_variant", "exon_variant", "exon_loss_variant", "frameshift_variant", "gene_variant", "intergenic_variant", "intergenic_region", "conserved_intergenic_variant", "intragenic_variant", "intron_variant", "conserved_intron_variant", "missense_variant", "rare_amino_acid_variant", "splice_acceptor_variant", "splice_donor_variant", "splice_region_variant", "5_prime_UTR_premature_start_codon_variant", "start_lost", "stop_gained", "stop_lost", "synonymous_variant", "start_retained_variant", "stop_retained_variant", "transcript_variant", "upstream_gene_variant", "3_prime_UTR_truncation_+_exon_loss_variant", "3_prime_UTR_variant", "5_prime_UTR_truncation_+_exon_loss_variant", "5_prime_UTR_variant", "initiator_codon_variant", "None", "chromosomal_deletion" |
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81 ) |
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82 names(classic_snpeff_effects_to_so) <- c( |
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83 "CDS", "CODON_CHANGE", "CODON_CHANGE_PLUS_CODON_DELETION", "CODON_CHANGE_PLUS_CODON_INSERTION", "CODON_DELETION", "CODON_INSERTION", "DOWNSTREAM", "EXON", "EXON_DELETED", "FRAME_SHIFT", "GENE", "INTERGENIC", "INTERGENIC_REGION", "INTERGENIC_CONSERVED", "INTRAGENIC", "INTRON", "INTRON_CONSERVED", "NON_SYNONYMOUS_CODING", "RARE_AMINO_ACID", "SPLICE_SITE_ACCEPTOR", "SPLICE_SITE_DONOR", "SPLICE_SITE_REGION", "START_GAINED", "START_LOST", "STOP_GAINED", "STOP_LOST", "SYNONYMOUS_CODING", "SYNONYMOUS_START", "SYNONYMOUS_STOP", "TRANSCRIPT", "UPSTREAM", "UTR_3_DELETED", "UTR_3_PRIME", "UTR_5_DELETED", "UTR_5_PRIME", "NON_SYNONYMOUS_START", "NONE", "CHROMOSOME_LARGE_DELETION" |
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84 ) |
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85 # translate classic effects into SO terms leaving unknown terms (possibly SO already) as is |
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86 so_effects <- sapply(ann_final$effect, function(x) trans(x, classic_snpeff_effects_to_so)) |
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87 |
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88 # handle further translation of effects we care about |
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89 so_effects_translation <- list( |
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90 "non-syn", "syn", |
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91 "deletion", "deletion", "deletion", |
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92 "insertion", "insertion", "frame shift", |
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93 "stop gained", "stop lost" |
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94 ) |
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95 names(so_effects_translation) <- c( |
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96 "missense_variant", "synonymous_variant", |
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97 "disruptive_inframe_deletion", "inframe_deletion", "chromosomal_deletion", |
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98 "disruptive_inframe_insertion", "inframe_insertion", "frameshift_variant", |
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99 "stop_gained", "stop_lost" |
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100 ) |
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101 # translate to our simple terms turning undefined terms into '?' |
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102 simple_effects <- sapply(so_effects, function(x) trans(x, so_effects_translation, replace_missing = "?")) |
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103 # complex variant effects (those that do more than one thing) are concatenated |
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104 # with either '+' (for classic terms) or '&' (for SO terms) |
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105 simple_effects[grepl("+", so_effects, fixed = TRUE)] <- "complex" |
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106 simple_effects[grepl("&", so_effects, fixed = TRUE)] <- "complex" |
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107 simple_effects[so_effects == ""] <- "non-coding" |
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108 |
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109 ann_final$effect <- simple_effects |
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110 ann_final$gene <- sub("^$", "NCR", ann_final$gene) |
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111 |
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112 ## automatically determine gaps for the heatmap |
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113 gap_vector <- which(!(ann_final$gene[1:length(ann_final$gene) - 1] == # nolint |
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114 ann_final$gene[2:length(ann_final$gene)])) |
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115 |
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116 # colormanagement |
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117 my_colors <- colorRampPalette(c("grey93", "brown", "black")) #heatmap |
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118 count <- length(unique(ann_final$gene)) #annotations (genes) |
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119 gene_color <- c(brewer.pal(brewer_color_gene_annotation, n = count)) |
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120 names(gene_color) <- unique(ann_final$gene) |
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121 |
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122 # colormanagement annotations (effect) |
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123 ## Define the full set of colors for each effect that we can encounter |
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124 ## This is not bulletproof. The effect names given here were swapped into the |
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125 ## data (see above substitutions in ann_final$effect) and so are hard-coded, |
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126 ## as well as their preferred colors. |
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127 |
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128 all_colors <- data.frame( |
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129 color = c("white", "green", "orange", "red", |
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130 "black", "grey", "yellow", "blue", "purple", "brown"), |
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131 name = c("non-coding", "syn", "non-syn", "deletion", |
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132 "frame shift", "stop gained", "stop lost", "insertion", |
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133 "complex", "?")) |
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134 ## Reduce the full set to just those that we want |
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135 detected_effects <- unique(ann_final$effect) |
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136 subset_colors <- subset(all_colors, name %in% detected_effects) |
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137 effect_color <- subset_colors$color |
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138 names(effect_color) <- subset_colors$name |
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139 color_list <- list(gene_color = gene_color, effect_color = effect_color) |
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140 names(color_list) <- c("gene", "effect") |
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141 |
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142 # visualize heatmap |
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143 if (pheat_number_of_clusters > length(samples$ids)) { |
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144 print(paste0("[INFO] Number of clusters: User-specified clusters (", |
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145 pheat_number_of_clusters, |
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146 ") is greater than the number of samples (", |
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147 length(samples$ids), ")")) |
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148 pheat_number_of_clusters <- length(samples$ids) |
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149 print(paste0("[INFO] Number of clusters: now set to ", |
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150 pheat_number_of_clusters)) |
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151 } |
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152 |
1
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153 |
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154 # Fix Labels |
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155 ## Prettify names, check for label parity between final and ann_final |
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156 fix_label <- function(name) { |
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157 ##' Reduce: 424 AGTAGAAGTTGAAAAAGGCGTTTTGCCTCAACTT A |
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158 ##' to: 424 AGT… > A |
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159 cols <- unlist(str_split(name, " ")) |
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160 ## first 3 are POS REF ALT, and the rest are optional differences |
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161 pos_ref_alt <- cols[1:3] |
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162 rest <- "" |
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163 if (length(cols) > 3) { |
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164 rest <- paste0(" :: ", paste(cols[4:length(cols)], sep = " ")) |
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165 } |
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166 ## Trim the REF or ALT if too long |
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167 if (str_length(pos_ref_alt[2]) > 3) { |
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168 pos_ref_alt[2] <- paste0(substring(pos_ref_alt[2], 1, 3), "…") |
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169 } |
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170 if (str_length(pos_ref_alt[3]) > 3) { |
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171 pos_ref_alt[3] <- paste0(substring(pos_ref_alt[3], 1, 3), "…") |
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172 } |
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173 ## Join required |
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174 new_name <- paste0(pos_ref_alt[1], " ", |
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175 pos_ref_alt[2], " > ", |
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176 pos_ref_alt[3]) |
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177 ## Join rest |
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178 new_name <- paste0(new_name, " ", paste(rest)) |
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179 } |
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180 |
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181 colnames(final) <- sapply(colnames(final), fix_label) |
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182 rownames(ann_final) <- sapply(rownames(ann_final), fix_label) |
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183 ## sanity test |
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184 stopifnot(all(colnames(final) %in% rownames(ann_final))) |
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185 |
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186 |
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187 # Perform Plotting |
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188 get_plot_dims <- function(heat_map) { |
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189 ## get the dimensions of a pheatmap object |
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190 ## useful for plot formats that can't be written to a file directly, but |
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191 ## for which we need to set up a plotting device |
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192 ## source: https://stackoverflow.com/a/61876386 |
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193 plot_height <- sum(sapply(heat_map$gtable$heights, |
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194 grid::convertHeight, "in")) |
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195 plot_width <- sum(sapply(heat_map$gtable$widths, |
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196 grid::convertWidth, "in")) |
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197 return(list(height = plot_height, width = plot_width)) |
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198 } |
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199 |
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200 height <- round(max(c(max(c( |
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201 16 * (length(unique(ann_final$effect)) + |
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202 length(unique(ann_final$gene))), 160)) / |
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203 nrow(final), 15))) |
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204 width <- round(ratio * height) |
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205 |
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206 |
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207 if (!(out_ext %in% c("svg", "jpeg", "png", "pdf"))) { |
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208 stop("Unknown extension: ", ext, ", aborting.") |
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209 } |
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210 plot_device <- get(out_ext) |
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211 |
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212 |
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213 ## A constant scaling factor based on the calculated dimensions |
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214 ## above does not work for PNG, so we resort to feeding pheatmap |
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215 ## with a direct filename |
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216 plot_filename <- NA |
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217 if (out_ext %in% c("jpeg", "png")) { |
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218 plot_filename <- out_file |
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219 } |
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220 |
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221 ## SVG is not a format pheatmap knows how to write to a file directly. |
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222 ## As a workaround we |
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223 ## 1. create the plot object |
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224 ## 2. get its dimensions |
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225 ## 3. set up a svg plotting device with these dimensions |
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226 ## 4. print the heatmap object to the device |
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227 hm <- pheatmap( |
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228 final, |
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229 color = my_colors(100), |
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230 cellwidth = width, |
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231 cellheight = height, |
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232 fontsize_col = round(1 / 3 * width), |
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233 fontsize_row = round(1 / 3 * min(c(height, width))), |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
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234 clustering_method = pheat_clustering_method, |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
iuc
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235 cluster_rows = pheat_clustering, |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
iuc
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236 cluster_cols = F, |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
iuc
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237 cutree_rows = pheat_number_of_clusters, |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
iuc
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238 annotation_col = ann_final, |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
iuc
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239 annotation_colors = color_list, |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
iuc
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240 filename = plot_filename, |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
iuc
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241 gaps_col = gap_vector |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
iuc
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242 ) |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
iuc
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243 |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
iuc
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244 if (out_ext %in% c("pdf", "svg")) { |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
iuc
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245 plot_dims <- get_plot_dims(hm) |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
iuc
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246 plot_device(out_file, |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
iuc
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247 width = plot_dims$width, |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
iuc
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248 height = plot_dims$height) |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
iuc
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249 print(hm) |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
iuc
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250 dev.off() |
1062d6ad6503
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/snpfreqplot/ commit 1f35303af979c16d9a3126dbc882a59f686ace5d"
iuc
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251 } |