view sam_dump.xml @ 3:4732693f4661 draft

planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sra-tools commit f30369bef4e2cef17395cdd62ce81768c93f5edd

<tool id="sam_dump" name="Extract reads" version="@VERSION@">
    <description>in SAM or BAM format from NCBI SRA.</description>
    <macros>
        <import>sra_macros.xml</import>
    </macros>
    <expand macro="requirements"/>
    <version_command>sam-dump --version</version_command>
    <command>
        <![CDATA[
    #if $input.input_select=="file_list":
      for acc in `cat $input.file_list` ;
      do
    #elif $input.input_select=="accession_number":
      acc="$input.accession" &&
    #end if

    #if $input.input_select=="file_list" or $input.input_select=="accession_number":
          [ ""\$acc" =~ ^[E|S|D]RR[0-9]{1,}$" ] && (
    #end if



        ## Need to set the home directory to the current working directory,
        ## else the tool tries to write to home/.ncbi and fails when used
        ## with a cluster manager.
        export HOME=\$PWD &&
        vdb-config --restore-defaults &&
        vdb-config -s "/repository/user/main/public/root=\$PWD" &&
        ## Do not use prefetch if region is specified, to avoid downloading
        ## the complete sra file.
        #if ( str( $adv.region ) == "" ):
            ASCP_PATH=`command -v ascp` &&
            ASCP_KEY=`dirname \$ASCP_PATH`/asperaweb_id_dsa.openssh || true &&
            prefetch -X 200G --ascp-path "\$ASCP_PATH|\$ASCP_KEY" "\$acc" &&
            ## Duplicate vdb-config, in case settings changed between prefetch and
            ## dump command.
            vdb-config -s "/repository/user/main/public/root=\$PWD" &&
        #end if
        sam-dump --log-level fatal --disable-multithreading
        #if str( $adv.region ) != "":
            --aligned-region "$adv.region"
        #end if
        #if str( $adv.matepairDist ) != "":
            --matepair-distance "$adv.matepairDist"
        #end if
        #if str( $adv.minMapq ) != "":
            --min-mapq "$adv.minMapq"
        #end if
        #if str( $adv.header ) == "yes":
            --header
        #else:
            --no-header
        #end if
        #if str( $adv.alignments ) == "both":
            --unaligned
        #end if
        #if str( $adv.alignments ) == "unaligned":
            --unaligned-spots-only
        #end if
        #if (str( $adv.primary ) == "yes") and (str ( $adv.alignments != "unaligned") ):
            --primary
        #end if
        #if $input.input_select == "file":
            "$input.file"
        #elif $input.input_select == "accession_number":
            "\$acc"
        #elif $input.input_select=="file_list":
            "\$acc"
        #end if

        #if str( $outputformat ) == "bam":
            | samtools view -Sb - 2> /dev/null
        #end if
        #if $input.input_select == "file":
            > "$output_file"
        #elif $input.input_select == "accession_number":
            > "$output_accession" )
        #end if

        #if $input.input_select=="file_list":
                 #if str( $outputformat ) == "bam":
                      > "\$acc.bam"
                 #elif str( $outputformat ) == "sam":
                      > "\$acc.sam"
                 #end if
        ) ; done
        #end if


        ]]>
    </command>
    <inputs>
        <expand macro="input_conditional"/>
        <param name="outputformat" type="select" label="select output format">
            <option value="bam">bam</option>
            <option value="sam">sam</option>
        </param>
        <section name="adv" title="Advanced Options" expanded="False">
            <param name="header" type="select" value="yes">
                <label>output header</label>
                <option value="yes">Yes</option>
                <option value="no">No</option>
            </param>
            <expand macro="alignments"/>
            <expand macro="region"/>
            <expand macro="matepairDist"/>
            <param name="primary" type="select" value="no">
                <label>only primary aligments</label>
                <option value="no">No</option>
                <option value="yes">Yes</option>
            </param>
            <expand macro="minMapq"></expand>
        </section>
    </inputs>
    <outputs>
        <collection name="output_collection" type='list'>
          <filter>input['input_select'] == "file_list"</filter>
          <discover_datasets pattern="(?P&lt;designation&gt;.+)\.bam" directory="." ext='bam'/>
          <discover_datasets pattern="(?P&lt;designation&gt;.+)\.sam" directory="." ext='sam'/>
        </collection>
        <data name="output_accession" format="bam" label="${input.accession}.${outputformat}">
            <filter>input['input_select'] == "accession_number"</filter>
            <change_format>
                <when input="outputformat" value="sam" format="sam"/>
            </change_format>
        </data>
        <data name="output_file" format="bam" label="${input.file.name}.${outputformat}">
            <filter>input['input_select'] == "file"</filter>
            <change_format>
                <when input="outputformat" value="sam" format="sam"/>
            </change_format>
        </data>
    </outputs>
    <tests>
        <test>
            <param name="input_select" value="accession_number"/>
            <param name="accession" value="SRR925743"/>
            <param name="outputformat" value="sam"/>
            <param name="region" value="17:41243452-41277500"/>
            <output name="output_accession" file="sam_dump_result.sam" compare="contains" ftype="sam"/>
        </test>
    </tests>
    <help>
        This tool extracts reads from sra archives using sam-dump.
        The sam-dump program is developed at NCBI, and is available at
        http://www.ncbi.nlm.nih.gov/Traces/sra/sra.cgi?view=software.
        @SRATOOLS_ATTRRIBUTION@
    </help>
    <expand macro="citation"/>
</tool>