Mercurial > repos > iuc > stacks2_ustacks
diff check_bcfile.py @ 2:d033e1ccb386 draft
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit f55e2407891a3c1f73f14a77b7ddadcd6f5eb1f8"
author | iuc |
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date | Thu, 16 Jul 2020 07:31:44 -0400 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/check_bcfile.py Thu Jul 16 07:31:44 2020 -0400 @@ -0,0 +1,45 @@ +#!/usr/bin/env python + +import argparse +import sys + +parser = argparse.ArgumentParser() +parser.add_argument('bcfile', help='barcode file') +args = parser.parse_args() + +barcodes = [] + +with open(args.bcfile, "r") as fh: + for line in fh: + if len(line) == 0: + continue + if line.startswith("#"): + continue + barcodes.append(line.split()) + +if len(barcodes) <= 1: + sys.exit("barcode file is empty") + +# check that all lines have the same number of columns +ncol = None +for bc in barcodes: + if ncol is None: + ncol = len(bc) + elif ncol != len(bc): + sys.exit("barcode file has inconsistent number of columns") + +isname = False +for bc in barcodes: + if len(bc[-1].strip("ATCGatcg")) > 0: + isname = True + break + +names = set() +for bc in barcodes: + if isname: + n = bc[-1] + else: + n = '-'.join(bc) + if n in names: + sys.exit("duplicate sample %s in barcode file" % n) + names.add(n)