view stacks_assembleperead.xml @ 12:6eaf0f9836c8 draft

"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks commit 5f2ec13ecca435abaac2b99ba21f1d6497ec7139"

<tool id="stacks_assembleperead" name="Stacks: assemble read pairs by locus" version="@WRAPPER_VERSION@.1">
    <description>run the STACKS sort_read_pairs.pl and exec_velvet.pl wrappers</description>
    <expand macro="bio_tools"/>
    <macros>
        <import>macros.xml</import>
    </macros>
    <expand macro="requirements" />
    <expand macro="stdio"/>
    <command><![CDATA[

        mkdir stacks_inputs reads stacks_outputs
        &&
        #for $input_file in $stacks_col
            #set $ext = ""
            #if not str($input_file.element_identifier).endswith('.tsv')
                #set $ext = ".tsv"
            #end if
            ln -s '${input_file}' 'stacks_inputs/${input_file.element_identifier}${ext}' &&
        #end for

        #for $input_file in $reads
            #set $name = str($input_file.element_identifier)
            ## sort_read_pairs is expecting strange fastq names: <sample_name>.fq_2
            #if $name.endswith('.1.fq')
                ## handle a common case
                #set $name = $name[:-5]+".fq_1"
            #else if $name.endswith('.2.fq')
                ## handle a common case
                #set $name = $name[:-5]+".fq_2"
            #else if not $name.endswith('.fq') and not $name.endswith('.fq_2')
                ## no extension, consider it's a fq_2 file
                #set $name = $name + ".fq_2"
            #end if
            ln -s '${input_file}' 'reads/${name}' &&
        #end for

        sort_read_pairs.pl
            -p stacks_inputs
            -s 'reads'

            #if $whitelist
                -w '$whitelist'
            #end if

            #if $threshold
                -r $threshold
            #end if

            -o stacks_outputs

        #if $velvet.use_velvet == "yes"
            ## remove possible empty files
            && find stacks_outputs -type f -size 0 -exec rm {} \;

            &&
            mkdir assembled
            &&
            velvet_path=`which velveth` && velvet_path=`dirname "\$velvet_path"`
            &&
            exec_velvet.pl -s stacks_outputs -o assembled -c -M ${velvet.contig_length} -e "\$velvet_path"
        #end if

    ]]></command>
    <inputs>
        <param name="stacks_col" argument="-p" format="tabular,txt" type="data_collection" collection_type="list" label="Output from previous Stacks pipeline steps (e.g. denovo_map or refmap)" />
        <param name="reads" argument="-s" format="fastqsanger" type="data" multiple="true" label="Files containing reads to assemble" help="only R2 reads" />

        <param name="whitelist" argument="-w" format="txt,tabular" type="data" optional="true" label="White list of catalog IDs to include" />
        <param name="threshold" argument="-r" type="integer" value="" optional="true" label="Minimum number of reads by locus"/>

       <conditional name="velvet">
            <param name="use_velvet" type="select" label="Perform assembly with Velvet" help="If not selected, the tool will only produce of collection of fasta files (one per locus) containing reads ready to assemble.">
                <option value="no" selected="true">No</option>
                <option value="yes">Yes</option>
            </param>
            <when value="no"/>
            <when value="yes">
                <param name="contig_length" type="integer" value="200" label="Minimum length for asssembled contigs"/>
            </when>
        </conditional>
    </inputs>
    <outputs>
        <collection name="collated" type="list" label="Collated FASTA files per locus on ${on_string}">
            <filter>velvet['use_velvet'] == "no"</filter>
            <discover_datasets pattern="(?P&lt;name&gt;.+)\.fa(sta)?$" ext="fasta" directory="stacks_outputs" />
        </collection>

        <data format="fasta" name="contigs" label="Assembled contigs on ${on_string}" from_work_dir="assembled/collated.fa">
            <filter>velvet['use_velvet'] == "yes"</filter>
        </data>
    </outputs>

    <tests>
        <test>
            <param name="stacks_col">
                <collection type="list">
                    <element name="batch_1.catalog.alleles.tsv" ftype="tabular" value="genotypes/batch_1.catalog.alleles.tsv" />
                    <element name="batch_1.catalog.snps.tsv" ftype="tabular" value="genotypes/batch_1.catalog.snps.tsv" />
                    <element name="batch_1.catalog.tags.tsv" ftype="tabular" value="genotypes/batch_1.catalog.tags.tsv" />
                    <element name="PopA_01.alleles.tsv" ftype="tabular" value="genotypes/PopA_01.alleles.tsv" />
                    <element name="PopA_01.matches.tsv" ftype="tabular" value="genotypes/PopA_01.matches.tsv" />
                    <element name="PopA_01.snps.tsv" ftype="tabular" value="genotypes/PopA_01.snps.tsv" />
                    <element name="PopA_01.tags.tsv" ftype="tabular" value="genotypes/PopA_01.tags.tsv" />
                    <element name="PopA_02.alleles.tsv" ftype="tabular" value="genotypes/PopA_02.alleles.tsv" />
                    <element name="PopA_02.matches.tsv" ftype="tabular" value="genotypes/PopA_02.matches.tsv" />
                    <element name="PopA_02.snps.tsv" ftype="tabular" value="genotypes/PopA_02.snps.tsv" />
                    <element name="PopA_02.tags.tsv" ftype="tabular" value="genotypes/PopA_02.tags.tsv" />
                </collection>
            </param>
            <param name="reads" value="demultiplexed/PopA_01.2.fq,demultiplexed/PopA_02.2.fq" ftype="fastqsanger" />

            <output_collection name="collated">
                <element name="1">
                    <assert_contents>
                        <has_text text="CCGATCAGCATCAGTAGTTTTCAACGAGCTGGCCCAATGGTGTATAACTATGTGGTAGAGAGAAACTGCTGCTATCACTCACGATATAAGCCCTCTGACG" />
                    </assert_contents>
                </element>
            </output_collection>
        </test>
        <test>
            <param name="stacks_col">
                <collection type="list">
                    <element name="batch_1.catalog.alleles.tsv" ftype="tabular" value="genotypes/batch_1.catalog.alleles.tsv" />
                    <element name="batch_1.catalog.snps.tsv" ftype="tabular" value="genotypes/batch_1.catalog.snps.tsv" />
                    <element name="batch_1.catalog.tags.tsv" ftype="tabular" value="genotypes/batch_1.catalog.tags.tsv" />
                    <element name="PopA_01.alleles.tsv" ftype="tabular" value="genotypes/PopA_01.alleles.tsv" />
                    <element name="PopA_01.matches.tsv" ftype="tabular" value="genotypes/PopA_01.matches.tsv" />
                    <element name="PopA_01.snps.tsv" ftype="tabular" value="genotypes/PopA_01.snps.tsv" />
                    <element name="PopA_01.tags.tsv" ftype="tabular" value="genotypes/PopA_01.tags.tsv" />
                    <element name="PopA_02.alleles.tsv" ftype="tabular" value="genotypes/PopA_02.alleles.tsv" />
                    <element name="PopA_02.matches.tsv" ftype="tabular" value="genotypes/PopA_02.matches.tsv" />
                    <element name="PopA_02.snps.tsv" ftype="tabular" value="genotypes/PopA_02.snps.tsv" />
                    <element name="PopA_02.tags.tsv" ftype="tabular" value="genotypes/PopA_02.tags.tsv" />
                </collection>
            </param>
            <param name="reads" value="demultiplexed/PopA_01.2.fq,demultiplexed/PopA_02.2.fq" ftype="fastqsanger" />
            <param name="velvet|use_velvet" value="yes" />
            <param name="velvet|contig_length" value="20" />

            <output name="contigs">
                <assert_contents>
                    <has_text text="|NODE_" />
                </assert_contents>
            </output>
        </test>
    </tests>

    <help>
<![CDATA[
.. class:: infomark

**What it does**

This program will run each of the Stacks sort_read_pairs.pl and exec_velvet.pl utilities to assemble pair-end reads from STACKS pipeline results

--------

**Input file**

Output from denovo_map or ref_map


**Output file**

A collated.fa file containing assembled contigs for each locus

@STACKS_INFOS@
]]>
    </help>
    <expand macro="citation" />
</tool>