Mercurial > repos > iuc > stringtie
changeset 0:62d212192002 draft
Imported from capsule None
author | iuc |
---|---|
date | Thu, 03 Jul 2014 18:38:02 -0400 |
parents | |
children | 9f80c71f1779 |
files | stringtie.xml test-data/._stringtie_in1.bam test-data/._stringtie_out2.gtf test-data/stringtie_in1.bam test-data/stringtie_out1.gtf test-data/stringtie_out2.gtf tool_dependencies.xml |
diffstat | 7 files changed, 422 insertions(+), 0 deletions(-) [+] |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/stringtie.xml Thu Jul 03 18:38:02 2014 -0400 @@ -0,0 +1,117 @@ +<?xml version="1.0"?> +<tool name="StringTie" id="stringtie" version="1.0.0"> + <description>RNA-Seq assembler</description> + <requirements> + <requirement type="package" version="0.97">stringtie</requirement> + </requirements> + <command> +<![CDATA[ +stringtie "$input_bam" +-o "$output_gtf" +-p "\${GALAXY_SLOTS:-1}" +#if str($guide.use_guide) == 'yes': + -G "$guide.guide_gff" $guide.input_estimation + -C "$coverage" +#end if +#if str($option_set.options) == 'advanced': + -f "$option_set.fraction" + -m "$option_set.min_tlen" + -a "$option_set.min_anchor_len" + -j "$option_set.min_anchor_cov" + -c "$option_set.min_bundle_cov" + -s "$option_set.maxcov" + -g "$option_set.bdist" + -M "$option_set.bundle_fraction" $option_set.sensitive $option_set.disable_trimming +#end if +]]> +</command> + <inputs> + <param name="input_bam" type="data" format="bam" label="BAM file to assemble" /> + <conditional name="guide"> + <param name="use_guide" type="select" label="Use GFF file to guide assembly"> + <option value="yes">Use GFF</option> + <option value="no" selected="True">Do not use GFF</option> + </param> + <when value="no" /> + <when value="yes"> + <param name="guide_gff" type="data" format="gtf,gff3" label="Reference annotation to use for guiding the assembly process" /> + <param name="input_estimation" type="boolean" truevalue="-e" falsevalue="" label="Perform abundance estimation only of input transcripts" /> + </when> + </conditional> + <conditional name="option_set"> + <param name="options" type="select" label="Options"> + <option value="default" selected="True">Use defaults</option> + <option value="advanced">Specify advanced options</option> + </param> + <when value="default" /> + <when value="advanced"> + <param name="disable_trimming" type="boolean" falsevalue="" truevalue="-t" label="Disable trimming of predicted transcripts based on coverage" /> + <param name="sensitive" type="boolean" truevalue="-S" falsevalue="" label="Increase sensitivity" /> + <param name="label" type="text" value="STRG" label="Name prefix for output transcripts" /> + <param name="fraction" type="float" value="0.15" min="0.0" max="1.0" label="Minimum isoform fraction" /> + <param name="min_tlen" type="integer" value="200" label="Minimum assembled transcript length" /> + <param name="min_anchor_len" type="integer" value="10" label="Minimum anchor length for junctions" /> + <param name="min_anchor_cov" type="integer" value="1" label="Minimum junction coverage" /> + <param name="min_bundle_cov" type="integer" value="2" label="Minimum bundle reads per bp coverage to consider for assembly" /> + <param name="maxcov" type="integer" value="1000000" label="Coverage saturation threshold" /> + <param name="bdist" type="integer" value="50" label="Gap between read mappings triggering a new bundle" /> + <param name="bundle_fraction" type="float" value="0.95" label="Fraction of bundle allowed to be covered by multi-hit reads" /> + </when> + </conditional> + </inputs> + <outputs> + <data name="output_gtf" format="gtf" label="${tool.name} on ${on_string}: Assembled transcripts"/> + <data name="coverage" format="gff3" label="${tool.name} on ${on_string}: Coverage"> + <filter>guide['use_guide'] == "yes"</filter> + </data> + </outputs> + <tests> + <test> + <param name="input_bam" value="stringtie_in1.bam" ftype="bam" /> + <param name="use_guide" value="no" /> + <param name="options" value="default" /> + <output name="output_gtf" file="stringtie_out1.gtf" ftype="gtf" /> + </test> + <test> + <param name="input_bam" value="stringtie_in1.bam" ftype="bam" /> + <param name="use_guide" value="no" /> + <param name="options" value="advanced" /> + <param name="fraction" value="0.17" /> + <output name="output_gtf" file="stringtie_out2.gtf" ftype="gtf" /> + </test> + </tests> + <help> +<![CDATA[ +StringTie v0.97 usage:: + + stringtie <input.bam> [-G <guide_gff>] [-l <label>] [-o <out_gff>] [-p <cpus>] + [-v] [-a <min_anchor_len>] [-m <min_tlen>] [-j <min_anchor_cov>] [-n sens] + [-C <coverage_file_name>] [-s <maxcov>] [-c <min_bundle_cov>] [-g <bdist>] + + Assemble RNA-Seq alignments into potential transcripts. + + Options: + -G reference annotation to use for guiding the assembly process (GTF/GFF3) + -l name prefix for output transcripts (default: STRG) + -f minimum isoform fraction (default: 0.15) + -m minimum assembled transcript length to report (default 200bp) + -o output file with the assembled transcripts (default: stdout) + -a minimum anchor length for junctions (default: 10) + -j minimum junction coverage (default: 1) + -t disable trimming of predicted transcripts based on coverage (default: trimming enabled) + -c minimum bundle reads per bp coverage to consider for assembly (default: 2) + -s coverage saturation threshold; further read alignments will be + ignored in a region where a local coverage depth of <maxcov> + is reached (default: 1,000,000); + -v verbose (log bundle processing details) + -e abundance estimation only of input transcripts (for -G option) + -g gap between read mappings triggering a new bundle (default: 50) + -S more sensitive run (default: no) + -C output file with all transcripts in reference that are fully + covered by reads + -M fraction of bundle allowed to be covered by multi-hit reads (default:0.95) + -p number of threads (CPUs) to use (default: 1) + +]]> +</help> +</tool> \ No newline at end of file
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/stringtie_out1.gtf Thu Jul 03 18:38:02 2014 -0400 @@ -0,0 +1,154 @@ +chr19 StringTie transcript 3052907 3062360 1000 - . gene_id "STRG.1"; transcript_id "STRG.1.1"; cov "883.073547";FPKM "189664.171875"; +chr19 StringTie exon 3052907 3054038 1000 - . gene_id "STRG.1"; transcript_id "STRG.1.1"; exon_number "1"; cov "966.177673"; +chr19 StringTie exon 3054118 3054192 1000 - . gene_id "STRG.1"; transcript_id "STRG.1.1"; exon_number "2"; cov "1301.127930"; +chr19 StringTie exon 3055662 3055724 1000 - . gene_id "STRG.1"; transcript_id "STRG.1.1"; exon_number "3"; cov "1990.478271"; +chr19 StringTie exon 3056310 3056354 1000 - . gene_id "STRG.1"; transcript_id "STRG.1.1"; exon_number "4"; cov "942.796082"; +chr19 StringTie exon 3057677 3057740 1000 - . gene_id "STRG.1"; transcript_id "STRG.1.1"; exon_number "5"; cov "351.962616"; +chr19 StringTie exon 3061158 3061255 1000 - . gene_id "STRG.1"; transcript_id "STRG.1.1"; exon_number "6"; cov "1122.192017"; +chr19 StringTie exon 3062172 3062360 1000 - . gene_id "STRG.1"; transcript_id "STRG.1.1"; exon_number "7"; cov "214.494049"; +chr19 StringTie transcript 3052907 3063089 1000 - . gene_id "STRG.1"; transcript_id "STRG.1.2"; cov "168.800812";FPKM "36254.585938"; +chr19 StringTie exon 3052907 3054038 1000 - . gene_id "STRG.1"; transcript_id "STRG.1.2"; exon_number "1"; cov "220.181381"; +chr19 StringTie exon 3054118 3054192 1000 - . gene_id "STRG.1"; transcript_id "STRG.1.2"; exon_number "2"; cov "258.932983"; +chr19 StringTie exon 3055662 3055724 1000 - . gene_id "STRG.1"; transcript_id "STRG.1.2"; exon_number "3"; cov "176.962967"; +chr19 StringTie exon 3056310 3056354 1000 - . gene_id "STRG.1"; transcript_id "STRG.1.2"; exon_number "4"; cov "118.451096"; +chr19 StringTie exon 3057677 3057740 1000 - . gene_id "STRG.1"; transcript_id "STRG.1.2"; exon_number "5"; cov "254.885681"; +chr19 StringTie exon 3061158 3061255 1000 - . gene_id "STRG.1"; transcript_id "STRG.1.2"; exon_number "6"; cov "78.436821"; +chr19 StringTie exon 3062698 3063089 1000 - . gene_id "STRG.1"; transcript_id "STRG.1.2"; exon_number "7"; cov "16.186066"; +chr19 StringTie transcript 2997664 3015854 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.1"; cov "115.851471";FPKM "24882.267578"; +chr19 StringTie exon 2997664 2997953 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.1"; exon_number "1"; cov "110.703667"; +chr19 StringTie exon 3000645 3000721 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.1"; exon_number "2"; cov "207.771072"; +chr19 StringTie exon 3002351 3002501 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.1"; exon_number "3"; cov "114.276024"; +chr19 StringTie exon 3005435 3005582 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.1"; exon_number "4"; cov "120.278976"; +chr19 StringTie exon 3005719 3005966 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.1"; exon_number "5"; cov "78.667053"; +chr19 StringTie exon 3006418 3006667 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.1"; 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exon_number "1"; cov "67.409538"; +chr19 StringTie exon 3110147 3110331 1000 + . gene_id "STRG.3"; transcript_id "STRG.3.1"; exon_number "2"; cov "102.204170"; +chr19 StringTie exon 3113328 3113482 1000 + . gene_id "STRG.3"; transcript_id "STRG.3.1"; exon_number "3"; cov "108.144516"; +chr19 StringTie exon 3114942 3115070 1000 + . gene_id "STRG.3"; transcript_id "STRG.3.1"; exon_number "4"; cov "63.896484"; +chr19 StringTie exon 3118922 3119121 1000 + . gene_id "STRG.3"; transcript_id "STRG.3.1"; exon_number "5"; cov "61.832138"; +chr19 StringTie transcript 3119186 3123995 1000 + . gene_id "STRG.4"; transcript_id "STRG.4.1"; cov "69.395134";FPKM "14904.501953"; +chr19 StringTie exon 3119186 3119357 1000 + . gene_id "STRG.4"; transcript_id "STRG.4.1"; exon_number "1"; cov "71.938263"; +chr19 StringTie exon 3120987 3123995 1000 + . gene_id "STRG.4"; transcript_id "STRG.4.1"; exon_number "2"; cov "69.249763"; +chr19 StringTie transcript 3136149 3163782 1000 + . gene_id "STRG.5"; transcript_id "STRG.5.1"; 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transcript_id "STRG.12.1"; exon_number "4"; cov "37.911308"; +chr19 StringTie exon 3198815 3198895 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.1"; exon_number "5"; cov "51.996696"; +chr19 StringTie exon 3201521 3201624 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.1"; exon_number "6"; cov "31.073275"; +chr19 StringTie exon 3203754 3203842 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.1"; exon_number "7"; cov "55.852036"; +chr19 StringTie exon 3204003 3204142 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.1"; exon_number "8"; cov "30.771446"; +chr19 StringTie exon 3204571 3204749 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.1"; exon_number "9"; cov "34.595020"; +chr19 StringTie exon 3205937 3206024 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.1"; exon_number "10"; cov "43.030930"; +chr19 StringTie exon 3206150 3206188 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.1"; exon_number "11"; cov "59.276905"; +chr19 StringTie exon 3206260 3206423 1000 + . gene_id "STRG.12"; 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transcript_id "STRG.12.2"; exon_number "12"; cov "20.523384"; +chr19 StringTie transcript 3203443 3209570 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.3"; cov "7.056122";FPKM "1515.494995"; +chr19 StringTie exon 3203443 3203842 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.3"; exon_number "1"; cov "2.242009"; +chr19 StringTie exon 3204003 3204142 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.3"; exon_number "2"; cov "3.895410"; +chr19 StringTie exon 3204571 3204749 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.3"; exon_number "3"; cov "6.615313"; +chr19 StringTie exon 3205937 3206024 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.3"; exon_number "4"; cov "10.143439"; +chr19 StringTie exon 3206150 3206188 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.3"; exon_number "5"; cov "11.954673"; +chr19 StringTie exon 3206260 3207249 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.3"; exon_number "6"; cov "3.642324"; +chr19 StringTie exon 3207389 3207467 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.3"; exon_number "7"; cov "5.884272"; +chr19 StringTie exon 3207627 3209570 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.3"; exon_number "8"; cov "9.862993"; +chr19 StringTie transcript 3363620 3364229 1000 . . gene_id "STRG.13"; transcript_id "STRG.13.1"; cov "2.622951";FPKM "563.350403"; +chr19 StringTie exon 3363620 3364229 1000 . . gene_id "STRG.13"; transcript_id "STRG.13.1"; exon_number "1"; cov "2.622951"; +chr19 StringTie transcript 3366538 3469274 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.1"; cov "71.175430";FPKM "15286.868164"; +chr19 StringTie exon 3366538 3366664 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.1"; exon_number "1"; cov "11.686789"; +chr19 StringTie exon 3381710 3382241 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.1"; exon_number "2"; cov "56.353500"; +chr19 StringTie exon 3425104 3425175 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.1"; exon_number "3"; cov "74.173615"; +chr19 StringTie exon 3433516 3433590 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.1"; exon_number "4"; cov "65.354446"; +chr19 StringTie exon 3434275 3434398 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.1"; exon_number "5"; cov "80.448212"; +chr19 StringTie exon 3435081 3435205 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.1"; exon_number "6"; cov "71.471077"; +chr19 StringTie exon 3449012 3449137 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.1"; exon_number "7"; cov "107.203087"; +chr19 StringTie exon 3452480 3452664 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.1"; exon_number "8"; cov "71.914742"; +chr19 StringTie exon 3453761 3453914 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.1"; exon_number "9"; cov "30.357872"; +chr19 StringTie exon 3456548 3456633 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.1"; exon_number "10"; cov "40.561024"; +chr19 StringTie exon 3462750 3469274 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.1"; exon_number "11"; cov "74.051750"; +chr19 StringTie transcript 3366538 3469274 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.2"; cov "18.338432";FPKM "3938.679688"; +chr19 StringTie exon 3366538 3366664 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.2"; exon_number "1"; cov "3.412646"; +chr19 StringTie exon 3381710 3382241 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.2"; exon_number "2"; cov "15.545184"; +chr19 StringTie exon 3425104 3425175 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.2"; exon_number "3"; cov "21.998638"; +chr19 StringTie exon 3433516 3433590 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.2"; exon_number "4"; cov "17.916565"; +chr19 StringTie exon 3434275 3434398 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.2"; exon_number "5"; cov "24.749645"; +chr19 StringTie exon 3435081 3435205 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.2"; exon_number "6"; cov "20.192204"; +chr19 StringTie exon 3449012 3449137 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.2"; exon_number "7"; cov "19.043257"; +chr19 StringTie exon 3452480 3452664 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.2"; exon_number "8"; cov "32.998787"; +chr19 StringTie exon 3462750 3469274 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.2"; exon_number "9"; cov "18.234524"; +chr19 StringTie transcript 3472911 3473297 1000 . . gene_id "STRG.15"; transcript_id "STRG.15.1"; cov "2.842377";FPKM "610.478088"; +chr19 StringTie exon 3472911 3473297 1000 . . gene_id "STRG.15"; transcript_id "STRG.15.1"; exon_number "1"; cov "2.842377"; +chr19 StringTie transcript 3473383 3473585 1000 . . gene_id "STRG.16"; transcript_id "STRG.16.1"; cov "2.216749";FPKM "476.107422"; +chr19 StringTie exon 3473383 3473585 1000 . . gene_id "STRG.16"; transcript_id "STRG.16.1"; exon_number "1"; cov "2.216749"; +chr19 StringTie transcript 3473881 3474130 1000 . . gene_id "STRG.17"; transcript_id "STRG.17.1"; cov "5.800000";FPKM "1245.708496"; +chr19 StringTie exon 3473881 3474130 1000 . . gene_id "STRG.17"; transcript_id "STRG.17.1"; exon_number "1"; cov "5.800000"; +chr19 StringTie transcript 3474201 3474891 1000 . . gene_id "STRG.18"; transcript_id "STRG.18.1"; cov "6.512301";FPKM "1398.694580"; +chr19 StringTie exon 3474201 3474891 1000 . . gene_id "STRG.18"; transcript_id "STRG.18.1"; exon_number "1"; cov "6.512301"; +chr19 StringTie transcript 3475786 3476529 1000 . . gene_id "STRG.19"; transcript_id "STRG.19.1"; cov "4.166667";FPKM "894.905579"; +chr19 StringTie exon 3475786 3476529 1000 . . gene_id "STRG.19"; transcript_id "STRG.19.1"; exon_number "1"; cov "4.166667"; +chr19 StringTie transcript 3474957 3475188 1000 - . gene_id "STRG.20"; transcript_id "STRG.20.1"; cov "3.719828";FPKM "798.934692"; +chr19 StringTie exon 3474957 3475188 1000 - . gene_id "STRG.20"; transcript_id "STRG.20.1"; exon_number "1"; cov "3.719828"; +chr19 StringTie transcript 3490820 3500661 1000 - . gene_id "STRG.21"; transcript_id "STRG.21.1"; cov "38.885868";FPKM "8351.802734"; +chr19 StringTie exon 3490820 3491809 1000 - . gene_id "STRG.21"; transcript_id "STRG.21.1"; exon_number "1"; cov "49.893391"; +chr19 StringTie exon 3492260 3492497 1000 - . gene_id "STRG.21"; transcript_id "STRG.21.1"; exon_number "2"; cov "14.960925"; +chr19 StringTie exon 3494026 3494102 1000 - . gene_id "STRG.21"; transcript_id "STRG.21.1"; exon_number "3"; cov "22.457817"; +chr19 StringTie exon 3496539 3496884 1000 - . gene_id "STRG.21"; transcript_id "STRG.21.1"; exon_number "4"; cov "35.878857"; +chr19 StringTie exon 3500559 3500661 1000 - . gene_id "STRG.21"; transcript_id "STRG.21.1"; exon_number "5"; cov "10.650763";
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/stringtie_out2.gtf Thu Jul 03 18:38:02 2014 -0400 @@ -0,0 +1,145 @@ +chr19 StringTie transcript 3052907 3062360 1000 - . gene_id "STRG.1"; transcript_id "STRG.1.1"; cov "883.073547";FPKM "189664.171875"; +chr19 StringTie exon 3052907 3054038 1000 - . gene_id "STRG.1"; transcript_id "STRG.1.1"; exon_number "1"; cov "966.177673"; +chr19 StringTie exon 3054118 3054192 1000 - . gene_id "STRG.1"; transcript_id "STRG.1.1"; exon_number "2"; cov "1301.127930"; +chr19 StringTie exon 3055662 3055724 1000 - . gene_id "STRG.1"; transcript_id "STRG.1.1"; exon_number "3"; cov "1990.478271"; +chr19 StringTie exon 3056310 3056354 1000 - . gene_id "STRG.1"; transcript_id "STRG.1.1"; exon_number "4"; cov "942.796082"; +chr19 StringTie exon 3057677 3057740 1000 - . gene_id "STRG.1"; transcript_id "STRG.1.1"; exon_number "5"; cov "351.962616"; +chr19 StringTie exon 3061158 3061255 1000 - . gene_id "STRG.1"; transcript_id "STRG.1.1"; exon_number "6"; cov "1122.192017"; +chr19 StringTie exon 3062172 3062360 1000 - . gene_id "STRG.1"; transcript_id "STRG.1.1"; exon_number "7"; cov "214.494049"; +chr19 StringTie transcript 3052907 3063089 1000 - . gene_id "STRG.1"; transcript_id "STRG.1.2"; cov "168.800812";FPKM "36254.585938"; +chr19 StringTie exon 3052907 3054038 1000 - . gene_id "STRG.1"; transcript_id "STRG.1.2"; exon_number "1"; cov "220.181381"; +chr19 StringTie exon 3054118 3054192 1000 - . gene_id "STRG.1"; transcript_id "STRG.1.2"; exon_number "2"; cov "258.932983"; +chr19 StringTie exon 3055662 3055724 1000 - . gene_id "STRG.1"; transcript_id "STRG.1.2"; exon_number "3"; cov "176.962967"; +chr19 StringTie exon 3056310 3056354 1000 - . gene_id "STRG.1"; transcript_id "STRG.1.2"; exon_number "4"; cov "118.451096"; +chr19 StringTie exon 3057677 3057740 1000 - . gene_id "STRG.1"; transcript_id "STRG.1.2"; exon_number "5"; cov "254.885681"; +chr19 StringTie exon 3061158 3061255 1000 - . gene_id "STRG.1"; transcript_id "STRG.1.2"; exon_number "6"; cov "78.436821"; +chr19 StringTie exon 3062698 3063089 1000 - . gene_id "STRG.1"; transcript_id "STRG.1.2"; exon_number "7"; cov "16.186066"; +chr19 StringTie transcript 2997664 3015854 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.1"; cov "115.851471";FPKM "24882.267578"; +chr19 StringTie exon 2997664 2997953 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.1"; exon_number "1"; cov "110.703667"; +chr19 StringTie exon 3000645 3000721 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.1"; exon_number "2"; cov "207.771072"; +chr19 StringTie exon 3002351 3002501 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.1"; exon_number "3"; cov "114.276024"; +chr19 StringTie exon 3005435 3005582 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.1"; exon_number "4"; cov "120.278976"; +chr19 StringTie exon 3005719 3005966 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.1"; exon_number "5"; cov "78.667053"; +chr19 StringTie exon 3006418 3006667 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.1"; exon_number "6"; cov "48.566441"; +chr19 StringTie exon 3008867 3008943 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.1"; exon_number "7"; cov "66.379005"; +chr19 StringTie exon 3009540 3009700 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.1"; exon_number "8"; cov "62.793331"; +chr19 StringTie exon 3011020 3011158 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.1"; exon_number "9"; cov "219.519562"; +chr19 StringTie exon 3013667 3013816 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.1"; exon_number "10"; cov "172.405579"; +chr19 StringTie exon 3014568 3014612 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.1"; exon_number "11"; cov "342.860687"; +chr19 StringTie exon 3015651 3015854 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.1"; exon_number "12"; cov "119.223740"; +chr19 StringTie transcript 3018960 3029141 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.2"; cov "18.253860";FPKM "3920.515381"; +chr19 StringTie exon 3018960 3019461 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.2"; exon_number "1"; cov "10.802196"; +chr19 StringTie exon 3019697 3019771 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.2"; exon_number "2"; cov "23.808632"; +chr19 StringTie exon 3025018 3025080 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.2"; exon_number "3"; cov "33.401360"; +chr19 StringTie exon 3027827 3027871 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.2"; exon_number "4"; cov "41.172310"; +chr19 StringTie exon 3028317 3028380 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.2"; exon_number "5"; cov "42.650341"; +chr19 StringTie exon 3028704 3028801 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.2"; exon_number "6"; cov "32.359097"; +chr19 StringTie exon 3028879 3029141 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.2"; exon_number "7"; cov "12.150498"; +chr19 StringTie transcript 3018960 3029141 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.3"; cov "5.218285";FPKM "1120.769287"; +chr19 StringTie exon 3018960 3019464 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.3"; exon_number "1"; cov "4.017776"; +chr19 StringTie exon 3019697 3019771 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.3"; exon_number "2"; cov "15.457561"; +chr19 StringTie exon 3025018 3025080 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.3"; exon_number "3"; cov "1.183362"; +chr19 StringTie exon 3027827 3027871 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.3"; exon_number "4"; cov "3.434842"; +chr19 StringTie exon 3028317 3028380 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.3"; exon_number "5"; cov "20.437429"; +chr19 StringTie exon 3028704 3028801 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.3"; exon_number "6"; cov "6.825138"; +chr19 StringTie exon 3028879 3029141 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.3"; exon_number "7"; cov "1.572921"; +chr19 StringTie transcript 3094613 3119121 1000 + . gene_id "STRG.3"; transcript_id "STRG.3.1"; cov "80.540161";FPKM "17298.201172"; +chr19 StringTie exon 3094613 3094785 1000 + . gene_id "STRG.3"; transcript_id "STRG.3.1"; exon_number "1"; cov "67.409538"; +chr19 StringTie exon 3110147 3110331 1000 + . gene_id "STRG.3"; transcript_id "STRG.3.1"; exon_number "2"; cov "102.204170"; +chr19 StringTie exon 3113328 3113482 1000 + . gene_id "STRG.3"; transcript_id "STRG.3.1"; exon_number "3"; cov "108.144516"; +chr19 StringTie exon 3114942 3115070 1000 + . gene_id "STRG.3"; transcript_id "STRG.3.1"; exon_number "4"; cov "63.896484"; +chr19 StringTie exon 3118922 3119121 1000 + . gene_id "STRG.3"; transcript_id "STRG.3.1"; exon_number "5"; cov "61.832138"; +chr19 StringTie transcript 3119186 3123995 1000 + . gene_id "STRG.4"; transcript_id "STRG.4.1"; cov "69.395134";FPKM "14904.501953"; +chr19 StringTie exon 3119186 3119357 1000 + . gene_id "STRG.4"; transcript_id "STRG.4.1"; exon_number "1"; cov "71.938263"; +chr19 StringTie exon 3120987 3123995 1000 + . gene_id "STRG.4"; transcript_id "STRG.4.1"; exon_number "2"; cov "69.249763"; +chr19 StringTie transcript 3136149 3163782 1000 + . gene_id "STRG.5"; transcript_id "STRG.5.1"; cov "13.414832";FPKM "2881.201660"; +chr19 StringTie exon 3136149 3136593 1000 + . gene_id "STRG.5"; transcript_id "STRG.5.1"; exon_number "1"; cov "2.243525"; +chr19 StringTie exon 3148589 3148773 1000 + . gene_id "STRG.5"; transcript_id "STRG.5.1"; exon_number "2"; cov "6.817567"; +chr19 StringTie exon 3150129 3150283 1000 + . gene_id "STRG.5"; transcript_id "STRG.5.1"; exon_number "3"; cov "12.169776"; +chr19 StringTie exon 3151705 3151833 1000 + . gene_id "STRG.5"; transcript_id "STRG.5.1"; exon_number "4"; cov "5.409668"; +chr19 StringTie exon 3155821 3155950 1000 + . gene_id "STRG.5"; transcript_id "STRG.5.1"; exon_number "5"; cov "22.124674"; +chr19 StringTie exon 3157726 3157879 1000 + . gene_id "STRG.5"; transcript_id "STRG.5.1"; exon_number "6"; cov "16.131796"; +chr19 StringTie exon 3162791 3163782 1000 + . gene_id "STRG.5"; transcript_id "STRG.5.1"; exon_number "7"; cov "19.328829"; +chr19 StringTie transcript 3176651 3177191 1000 . . gene_id "STRG.6"; transcript_id "STRG.6.1"; cov "2.218115";FPKM "476.400818"; +chr19 StringTie exon 3176651 3177191 1000 . . gene_id "STRG.6"; transcript_id "STRG.6.1"; exon_number "1"; cov "2.218115"; +chr19 StringTie transcript 3178008 3178218 1000 . . gene_id "STRG.7"; transcript_id "STRG.7.1"; cov "2.132701";FPKM "458.055786"; +chr19 StringTie exon 3178008 3178218 1000 . . gene_id "STRG.7"; transcript_id "STRG.7.1"; exon_number "1"; cov "2.132701"; +chr19 StringTie transcript 3178290 3182254 1000 . . gene_id "STRG.8"; transcript_id "STRG.8.1"; cov "25.143421";FPKM "5400.236816"; +chr19 StringTie exon 3178290 3182254 1000 . . gene_id "STRG.8"; transcript_id "STRG.8.1"; exon_number "1"; cov "25.143421"; +chr19 StringTie transcript 3182964 3183191 1000 . . gene_id "STRG.9"; transcript_id "STRG.9.1"; cov "3.289474";FPKM "706.504456"; +chr19 StringTie exon 3182964 3183191 1000 . . gene_id "STRG.9"; transcript_id "STRG.9.1"; exon_number "1"; cov "3.289474"; +chr19 StringTie transcript 3183534 3184980 1000 . . gene_id "STRG.10"; transcript_id "STRG.10.1"; cov "10.234969";FPKM "2198.239258"; +chr19 StringTie exon 3183534 3184980 1000 . . gene_id "STRG.10"; transcript_id "STRG.10.1"; exon_number "1"; cov "10.234969"; +chr19 StringTie transcript 3185059 3185762 1000 . . gene_id "STRG.11"; transcript_id "STRG.11.1"; cov "4.474432";FPKM "961.006531"; +chr19 StringTie exon 3185059 3185762 1000 . . gene_id "STRG.11"; transcript_id "STRG.11.1"; exon_number "1"; cov "4.474432"; +chr19 StringTie transcript 3185993 3209570 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.1"; cov "46.485111";FPKM "9983.947266"; +chr19 StringTie exon 3185993 3186212 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.1"; exon_number "1"; cov "9.429298"; +chr19 StringTie exon 3192468 3192658 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.1"; exon_number "2"; cov "21.059887"; +chr19 StringTie exon 3193282 3193426 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.1"; exon_number "3"; cov "39.715225"; +chr19 StringTie exon 3196181 3196275 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.1"; exon_number "4"; cov "37.911308"; +chr19 StringTie exon 3198815 3198895 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.1"; exon_number "5"; cov "51.996696"; +chr19 StringTie exon 3201521 3201624 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.1"; exon_number "6"; cov "31.073275"; +chr19 StringTie exon 3203754 3203842 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.1"; exon_number "7"; cov "55.852036"; +chr19 StringTie exon 3204003 3204142 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.1"; exon_number "8"; cov "30.771446"; +chr19 StringTie exon 3204571 3204749 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.1"; exon_number "9"; cov "34.595020"; +chr19 StringTie exon 3205937 3206024 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.1"; exon_number "10"; cov "43.030930"; +chr19 StringTie exon 3206150 3206188 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.1"; exon_number "11"; cov "59.276905"; +chr19 StringTie exon 3206260 3206423 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.1"; exon_number "12"; cov "46.387093"; +chr19 StringTie exon 3207196 3207249 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.1"; exon_number "13"; cov "63.118416"; +chr19 StringTie exon 3207389 3207467 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.1"; exon_number "14"; cov "65.674583"; +chr19 StringTie exon 3207627 3209570 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.1"; exon_number "15"; cov "54.248878"; +chr19 StringTie transcript 3195437 3209570 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.2"; cov "17.133051";FPKM "3679.790771"; +chr19 StringTie exon 3195437 3196275 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.2"; exon_number "1"; cov "10.562823"; +chr19 StringTie exon 3198815 3198895 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.2"; exon_number "2"; cov "17.045162"; +chr19 StringTie exon 3201521 3201624 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.2"; exon_number "3"; cov "9.639905"; +chr19 StringTie exon 3203754 3203842 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.2"; exon_number "4"; cov "14.507616"; +chr19 StringTie exon 3204003 3204142 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.2"; exon_number "5"; cov "5.000354"; +chr19 StringTie exon 3204571 3204749 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.2"; exon_number "6"; cov "10.209009"; +chr19 StringTie exon 3205937 3206024 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.2"; exon_number "7"; cov "16.365309"; +chr19 StringTie exon 3206150 3206188 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.2"; exon_number "8"; cov "25.572968"; +chr19 StringTie exon 3206263 3206423 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.2"; exon_number "9"; cov "19.185726"; +chr19 StringTie exon 3207196 3207249 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.2"; exon_number "10"; cov "38.189373"; +chr19 StringTie exon 3207389 3207467 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.2"; exon_number "11"; cov "31.688845"; +chr19 StringTie exon 3207627 3209570 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.2"; exon_number "12"; cov "20.523384"; +chr19 StringTie transcript 3363620 3364229 1000 . . gene_id "STRG.13"; transcript_id "STRG.13.1"; cov "2.622951";FPKM "563.350403"; +chr19 StringTie exon 3363620 3364229 1000 . . gene_id "STRG.13"; transcript_id "STRG.13.1"; exon_number "1"; cov "2.622951"; +chr19 StringTie transcript 3366538 3469274 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.1"; cov "71.175430";FPKM "15286.868164"; +chr19 StringTie exon 3366538 3366664 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.1"; exon_number "1"; cov "11.686789"; +chr19 StringTie exon 3381710 3382241 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.1"; exon_number "2"; cov "56.353500"; +chr19 StringTie exon 3425104 3425175 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.1"; exon_number "3"; cov "74.173615"; +chr19 StringTie exon 3433516 3433590 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.1"; exon_number "4"; cov "65.354446"; +chr19 StringTie exon 3434275 3434398 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.1"; exon_number "5"; cov "80.448212"; +chr19 StringTie exon 3435081 3435205 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.1"; exon_number "6"; cov "71.471077"; +chr19 StringTie exon 3449012 3449137 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.1"; exon_number "7"; cov "107.203087"; +chr19 StringTie exon 3452480 3452664 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.1"; exon_number "8"; cov "71.914742"; +chr19 StringTie exon 3453761 3453914 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.1"; exon_number "9"; cov "30.357872"; +chr19 StringTie exon 3456548 3456633 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.1"; exon_number "10"; cov "40.561024"; +chr19 StringTie exon 3462750 3469274 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.1"; exon_number "11"; cov "74.051750"; +chr19 StringTie transcript 3366538 3469274 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.2"; cov "18.338432";FPKM "3938.679688"; +chr19 StringTie exon 3366538 3366664 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.2"; exon_number "1"; cov "3.412646"; +chr19 StringTie exon 3381710 3382241 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.2"; exon_number "2"; cov "15.545184"; +chr19 StringTie exon 3425104 3425175 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.2"; exon_number "3"; cov "21.998638"; +chr19 StringTie exon 3433516 3433590 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.2"; exon_number "4"; cov "17.916565"; +chr19 StringTie exon 3434275 3434398 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.2"; exon_number "5"; cov "24.749645"; +chr19 StringTie exon 3435081 3435205 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.2"; exon_number "6"; cov "20.192204"; +chr19 StringTie exon 3449012 3449137 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.2"; exon_number "7"; cov "19.043257"; +chr19 StringTie exon 3452480 3452664 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.2"; exon_number "8"; cov "32.998787"; +chr19 StringTie exon 3462750 3469274 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.2"; exon_number "9"; cov "18.234524"; +chr19 StringTie transcript 3472911 3473297 1000 . . gene_id "STRG.15"; transcript_id "STRG.15.1"; cov "2.842377";FPKM "610.478088"; +chr19 StringTie exon 3472911 3473297 1000 . . gene_id "STRG.15"; transcript_id "STRG.15.1"; exon_number "1"; cov "2.842377"; +chr19 StringTie transcript 3473383 3473585 1000 . . gene_id "STRG.16"; transcript_id "STRG.16.1"; cov "2.216749";FPKM "476.107422"; +chr19 StringTie exon 3473383 3473585 1000 . . gene_id "STRG.16"; transcript_id "STRG.16.1"; exon_number "1"; cov "2.216749"; +chr19 StringTie transcript 3473881 3474130 1000 . . gene_id "STRG.17"; transcript_id "STRG.17.1"; cov "5.800000";FPKM "1245.708496"; +chr19 StringTie exon 3473881 3474130 1000 . . gene_id "STRG.17"; transcript_id "STRG.17.1"; exon_number "1"; cov "5.800000"; +chr19 StringTie transcript 3474201 3474891 1000 . . gene_id "STRG.18"; transcript_id "STRG.18.1"; cov "6.512301";FPKM "1398.694580"; +chr19 StringTie exon 3474201 3474891 1000 . . gene_id "STRG.18"; transcript_id "STRG.18.1"; exon_number "1"; cov "6.512301"; +chr19 StringTie transcript 3475786 3476529 1000 . . gene_id "STRG.19"; transcript_id "STRG.19.1"; cov "4.166667";FPKM "894.905579"; +chr19 StringTie exon 3475786 3476529 1000 . . gene_id "STRG.19"; transcript_id "STRG.19.1"; exon_number "1"; cov "4.166667"; +chr19 StringTie transcript 3474957 3475188 1000 - . gene_id "STRG.20"; transcript_id "STRG.20.1"; cov "3.719828";FPKM "798.934692"; +chr19 StringTie exon 3474957 3475188 1000 - . gene_id "STRG.20"; transcript_id "STRG.20.1"; exon_number "1"; cov "3.719828"; +chr19 StringTie transcript 3490820 3500661 1000 - . gene_id "STRG.21"; transcript_id "STRG.21.1"; cov "38.885868";FPKM "8351.802734"; +chr19 StringTie exon 3490820 3491809 1000 - . gene_id "STRG.21"; transcript_id "STRG.21.1"; exon_number "1"; cov "49.893391"; +chr19 StringTie exon 3492260 3492497 1000 - . gene_id "STRG.21"; transcript_id "STRG.21.1"; exon_number "2"; cov "14.960925"; +chr19 StringTie exon 3494026 3494102 1000 - . gene_id "STRG.21"; transcript_id "STRG.21.1"; exon_number "3"; cov "22.457817"; +chr19 StringTie exon 3496539 3496884 1000 - . gene_id "STRG.21"; transcript_id "STRG.21.1"; exon_number "4"; cov "35.878857"; +chr19 StringTie exon 3500559 3500661 1000 - . gene_id "STRG.21"; transcript_id "STRG.21.1"; exon_number "5"; cov "10.650763";
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/tool_dependencies.xml Thu Jul 03 18:38:02 2014 -0400 @@ -0,0 +1,6 @@ +<?xml version="1.0"?> +<tool_dependency> + <package name="stringtie" version="0.97"> + <repository changeset_revision="bbcdfb50d3f0" name="package_stringtie_0_97" owner="iuc" toolshed="http://toolshed.g2.bx.psu.edu" /> + </package> +</tool_dependency>