Mercurial > repos > iuc > stringtie
changeset 1:9f80c71f1779 draft
Uploaded updated tool wrapper for stringtie 1.0.1
author | iuc |
---|---|
date | Thu, 05 Mar 2015 11:41:12 -0500 |
parents | 62d212192002 |
children | 520e0988ec1c |
files | stringtie.xml test-data/._stringtie_in1.bam test-data/._stringtie_out2.gtf test-data/ballgown/e2t.ctab test-data/ballgown/e_data.ctab test-data/ballgown/i2t.ctab test-data/ballgown/i_data.ctab test-data/ballgown/t_data.ctab test-data/stringtie_in.gtf test-data/stringtie_in1.bam test-data/stringtie_out1.gtf test-data/stringtie_out2.gtf test-data/stringtie_out3.gtf test-data/stringtie_out4.gtf test-data/stringtie_out5.gtf test-data/stringtie_out_coverage.gtf tool_dependencies.xml |
diffstat | 17 files changed, 161 insertions(+), 365 deletions(-) [+] |
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--- a/stringtie.xml Thu Jul 03 18:38:02 2014 -0400 +++ b/stringtie.xml Thu Mar 05 11:41:12 2015 -0500 @@ -1,19 +1,20 @@ -<?xml version="1.0"?> -<tool name="StringTie" id="stringtie" version="1.0.0"> +<tool id="stringtie" name="StringTie" version="1.0.1"> <description>RNA-Seq assembler</description> <requirements> - <requirement type="package" version="0.97">stringtie</requirement> + <requirement type="package" version="1.0.1">stringtie</requirement> </requirements> - <command> -<![CDATA[ + <command><![CDATA[ stringtie "$input_bam" -o "$output_gtf" -p "\${GALAXY_SLOTS:-1}" #if str($guide.use_guide) == 'yes': - -G "$guide.guide_gff" $guide.input_estimation - -C "$coverage" + -C "$coverage" -G "$guide.guide_gff" $guide.input_estimation + #if str($guide.output_ballgown) == '-b': + $guide.output_ballgown `pwd` + #end if #end if #if str($option_set.options) == 'advanced': + -l "$option_set.name_prefix" -f "$option_set.fraction" -m "$option_set.min_tlen" -a "$option_set.min_anchor_len" @@ -26,92 +27,142 @@ ]]> </command> <inputs> - <param name="input_bam" type="data" format="bam" label="BAM file to assemble" /> + <param format="bam" label="BAM file to assemble" name="input_bam" type="data" /> <conditional name="guide"> - <param name="use_guide" type="select" label="Use GFF file to guide assembly"> + <param label="Use GFF file to guide assembly" name="use_guide" type="select"> <option value="yes">Use GFF</option> - <option value="no" selected="True">Do not use GFF</option> + <option selected="True" value="no">Do not use GFF</option> </param> <when value="no" /> <when value="yes"> - <param name="guide_gff" type="data" format="gtf,gff3" label="Reference annotation to use for guiding the assembly process" /> - <param name="input_estimation" type="boolean" truevalue="-e" falsevalue="" label="Perform abundance estimation only of input transcripts" /> + <param format="gtf,gff3" help="(-G)" label="Reference annotation to use for guiding the assembly process" name="guide_gff" type="data" /> + <param falsevalue="" help="(-e)" label="Perform abundance estimation only of input transcripts" name="input_estimation" truevalue="-e" type="boolean" /> + <param falsevalue="" help="(-b)" label="Output additional files for use in Ballgown" name="output_ballgown" truevalue="-b" type="boolean" /> </when> </conditional> <conditional name="option_set"> - <param name="options" type="select" label="Options"> - <option value="default" selected="True">Use defaults</option> + <param label="Options" name="options" type="select"> + <option selected="True" value="default">Use defaults</option> <option value="advanced">Specify advanced options</option> </param> <when value="default" /> <when value="advanced"> - <param name="disable_trimming" type="boolean" falsevalue="" truevalue="-t" label="Disable trimming of predicted transcripts based on coverage" /> - <param name="sensitive" type="boolean" truevalue="-S" falsevalue="" label="Increase sensitivity" /> - <param name="label" type="text" value="STRG" label="Name prefix for output transcripts" /> - <param name="fraction" type="float" value="0.15" min="0.0" max="1.0" label="Minimum isoform fraction" /> - <param name="min_tlen" type="integer" value="200" label="Minimum assembled transcript length" /> - <param name="min_anchor_len" type="integer" value="10" label="Minimum anchor length for junctions" /> - <param name="min_anchor_cov" type="integer" value="1" label="Minimum junction coverage" /> - <param name="min_bundle_cov" type="integer" value="2" label="Minimum bundle reads per bp coverage to consider for assembly" /> - <param name="maxcov" type="integer" value="1000000" label="Coverage saturation threshold" /> - <param name="bdist" type="integer" value="50" label="Gap between read mappings triggering a new bundle" /> - <param name="bundle_fraction" type="float" value="0.95" label="Fraction of bundle allowed to be covered by multi-hit reads" /> - </when> + <param falsevalue="" help="(-t)" label="Disable trimming of predicted transcripts based on coverage" name="disable_trimming" truevalue="-t" type="boolean" /> + <param falsevalue="" help="(-S)" label="Increase sensitivity" name="sensitive" truevalue="-S" type="boolean" /> + <param help="(-l)" label="Name prefix for output transcripts" name="name_prefix" type="text" value="STRG" /> + <param help="(-f)" label="Minimum isoform fraction" max="1.0" min="0.0" name="fraction" type="float" value="0.15" /> + <param help="(-m)" label="Minimum assembled transcript length" name="min_tlen" type="integer" value="200" /> + <param help="(-a)" label="Minimum anchor length for junctions" name="min_anchor_len" type="integer" value="10" /> + <param help="(-j)" label="Minimum junction coverage" name="min_anchor_cov" type="integer" value="1" /> + <param help="(-c)" label="Minimum bundle reads per bp coverage to consider for assembly" name="min_bundle_cov" type="integer" value="2" /> + <param help="(-s)" label="Coverage saturation threshold" name="maxcov" type="integer" value="1000000" /> + <param help="(-g)" label="Gap between read mappings triggering a new bundle" name="bdist" type="integer" value="50" /> + <param help="(-M)" label="Fraction of bundle allowed to be covered by multi-hit reads" name="bundle_fraction" type="float" value="0.95" /> + </when> </conditional> </inputs> <outputs> - <data name="output_gtf" format="gtf" label="${tool.name} on ${on_string}: Assembled transcripts"/> - <data name="coverage" format="gff3" label="${tool.name} on ${on_string}: Coverage"> + <data format="gtf" label="${tool.name} on ${on_string}: Assembled transcripts" name="output_gtf" /> + <data format="gff3" label="${tool.name} on ${on_string}: Coverage" name="coverage"> <filter>guide['use_guide'] == "yes"</filter> </data> + <data format="tabular" from_work_dir="e_data.ctab" label="${tool.name} on ${on_string}: exon-level expression measurements" name="exon_expression"> + <filter>guide['output_ballgown']</filter> + </data> + <data format="tabular" from_work_dir="i_data.ctab" label="${tool.name} on ${on_string}: intron-level expression measurements" name="intron_expression"> + <filter>guide['output_ballgown']</filter> + </data> + <data format="tabular" from_work_dir="t_data.ctab" label="${tool.name} on ${on_string}: transcript-level expression measurements" name="transcript_expression"> + <filter>guide['output_ballgown']</filter> + </data> + <data format="tabular" from_work_dir="e2t.ctab" label="${tool.name} on ${on_string}: exon to transcript mapping" name="exon_transcript_mapping"> + <filter>guide['output_ballgown']</filter> + </data> + <data format="tabular" from_work_dir="i2t.ctab" label="${tool.name} on ${on_string}: intron to transcript mapping" name="intron_transcript_mapping"> + <filter>guide['output_ballgown']</filter> + </data> </outputs> <tests> <test> - <param name="input_bam" value="stringtie_in1.bam" ftype="bam" /> + <param ftype="bam" name="input_bam" value="stringtie_in1.bam" /> <param name="use_guide" value="no" /> <param name="options" value="default" /> - <output name="output_gtf" file="stringtie_out1.gtf" ftype="gtf" /> + <output file="stringtie_out1.gtf" ftype="gtf" name="output_gtf" /> </test> <test> - <param name="input_bam" value="stringtie_in1.bam" ftype="bam" /> + <param ftype="bam" name="input_bam" value="stringtie_in1.bam" /> <param name="use_guide" value="no" /> <param name="options" value="advanced" /> <param name="fraction" value="0.17" /> - <output name="output_gtf" file="stringtie_out2.gtf" ftype="gtf" /> + <output file="stringtie_out2.gtf" ftype="gtf" name="output_gtf" /> + </test> + <test> + <param ftype="bam" name="input_bam" value="stringtie_in1.bam" /> + <param name="use_guide" value="yes" /> + <param name="guide_gff" value="stringtie_in.gtf" /> + <param name="options" value="default" /> + <output file="stringtie_out3.gtf" ftype="gtf" name="output_gtf" /> + </test> + <test> + <param ftype="bam" name="input_bam" value="stringtie_in1.bam" /> + <param name="use_guide" value="yes" /> + <param name="guide_gff" value="stringtie_in.gtf" /> + <param name="options" value="advanced" /> + <param name="fraction" value="0.17" /> + <output file="stringtie_out4.gtf" ftype="gtf" name="output_gtf" /> + </test> + <test> + <param ftype="bam" name="input_bam" value="stringtie_in1.bam" /> + <param name="use_guide" value="yes" /> + <param name="output_ballgown" value="yes" /> + <param name="guide_gff" value="stringtie_in.gtf" /> + <param name="options" value="default" /> + <output file="ballgown/e_data.ctab" ftype="tabular" name="exon_expression" /> + <output file="ballgown/i_data.ctab" ftype="tabular" name="intron_expression" /> + <output file="ballgown/t_data.ctab" ftype="tabular" name="transcript_expression" /> + <output file="ballgown/e2t.ctab" ftype="tabular" name="exon_transcript_mapping" /> + <output file="ballgown/i2t.ctab" ftype="tabular" name="intron_transcript_mapping" /> + <output file="stringtie_out5.gtf" ftype="gtf" name="output_gtf" /> + <output file="stringtie_out_coverage.gtf" ftype="gff3" name="coverage" /> </test> </tests> <help> <![CDATA[ -StringTie v0.97 usage:: - - stringtie <input.bam> [-G <guide_gff>] [-l <label>] [-o <out_gff>] [-p <cpus>] - [-v] [-a <min_anchor_len>] [-m <min_tlen>] [-j <min_anchor_cov>] [-n sens] - [-C <coverage_file_name>] [-s <maxcov>] [-c <min_bundle_cov>] [-g <bdist>] - - Assemble RNA-Seq alignments into potential transcripts. +StringTie v1.0.1 usage: + stringtie <input.bam> [-G <guide_gff>] [-l <label>] [-o <out_gtf>] [-p <cpus>] + [-v] [-a <min_anchor_len>] [-m <min_tlen>] [-j <min_anchor_cov>] [-n sens] + [-C <coverage_file_name>] [-s <maxcov>] [-c <min_bundle_cov>] [-g <bdist>] + {-B | -b <dir_path>} [-e] - Options: - -G reference annotation to use for guiding the assembly process (GTF/GFF3) - -l name prefix for output transcripts (default: STRG) - -f minimum isoform fraction (default: 0.15) - -m minimum assembled transcript length to report (default 200bp) - -o output file with the assembled transcripts (default: stdout) - -a minimum anchor length for junctions (default: 10) - -j minimum junction coverage (default: 1) - -t disable trimming of predicted transcripts based on coverage (default: trimming enabled) - -c minimum bundle reads per bp coverage to consider for assembly (default: 2) - -s coverage saturation threshold; further read alignments will be - ignored in a region where a local coverage depth of <maxcov> - is reached (default: 1,000,000); - -v verbose (log bundle processing details) - -e abundance estimation only of input transcripts (for -G option) - -g gap between read mappings triggering a new bundle (default: 50) - -S more sensitive run (default: no) - -C output file with all transcripts in reference that are fully - covered by reads - -M fraction of bundle allowed to be covered by multi-hit reads (default:0.95) - -p number of threads (CPUs) to use (default: 1) - -]]> -</help> -</tool> \ No newline at end of file +Assemble RNA-Seq alignments into potential transcripts. + + Options: + -G reference annotation to use for guiding the assembly process (GTF/GFF3) + -l name prefix for output transcripts (default: STRG) + -f minimum isoform fraction (default: 0.1) + -m minimum assembled transcript length to report (default 200bp) + -o output path/file name for the assembled transcripts GTF (default: stdout) + -a minimum anchor length for junctions (default: 10) + -j minimum junction coverage (default: 1) + -t disable trimming of predicted transcripts based on coverage + (default: coverage trimming is enabled) + -c minimum reads per bp coverage to consider for transcript assembly (default: 2.5) + -s coverage saturation threshold; further read alignments will be + ignored in a region where a local coverage depth of <maxcov> + is reached (default: 1,000,000); + -v verbose (log bundle processing details) + -g gap between read mappings triggering a new bundle (default: 50) + -C output file with reference transcripts that are covered by reads + -M fraction of bundle allowed to be covered by multi-hit reads (default:0.95) + -p number of threads (CPUs) to use (default: 1) + -B enable output of Ballgown table files which will be created in the + same directory as the output GTF (requires -G, -o recommended) + -b enable output of Ballgown table files but these files will be + created under the directory path given as <dir_path> + -e only estimates the abundance of given reference transcripts (requires -G) + ]]> + </help> + <citations> + <citation type="doi">doi:10.1038/nbt.3122</citation> + </citations> +</tool>
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/ballgown/e2t.ctab Thu Mar 05 11:41:12 2015 -0500 @@ -0,0 +1,4 @@ +e_id t_id +1 1 +2 1 +3 1
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/ballgown/e_data.ctab Thu Mar 05 11:41:12 2015 -0500 @@ -0,0 +1,4 @@ +e_id chr strand start end rcount ucount mrcount cov cov_sd mcov mcov_sd +1 test_chromosome + 53 250 154 154 154.00 49.7778 22.0747 49.7778 22.0747 +2 test_chromosome + 351 400 73 73 73.00 54.1600 6.1753 54.1600 6.1753 +3 test_chromosome + 501 550 38 38 38.00 21.6400 12.4350 21.6400 12.4350
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/ballgown/i2t.ctab Thu Mar 05 11:41:12 2015 -0500 @@ -0,0 +1,3 @@ +i_id t_id +1 1 +2 1
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/ballgown/i_data.ctab Thu Mar 05 11:41:12 2015 -0500 @@ -0,0 +1,3 @@ +i_id chr strand start end rcount ucount mrcount +1 test_chromosome + 251 350 49 49 49.00 +2 test_chromosome + 401 500 38 38 38.00
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/ballgown/t_data.ctab Thu Mar 05 11:41:12 2015 -0500 @@ -0,0 +1,2 @@ +t_id chr strand start end t_name num_exons length gene_id gene_name cov FPKM +1 test_chromosome + 53 550 CUFF.1.1 3 298 CUFF.1 . 44.724823 3276543.750000
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/stringtie_in.gtf Thu Mar 05 11:41:12 2015 -0500 @@ -0,0 +1,4 @@ +test_chromosome Cufflinks transcript 53 550 1000 + . gene_id "CUFF.1"; transcript_id "CUFF.1.1"; FPKM "10679134.4063403048"; frac "1.000000"; conf_lo "8542701.791788"; conf_hi "12815567.020892"; cov "145.770185"; +test_chromosome Cufflinks exon 53 250 1000 + . gene_id "CUFF.1"; transcript_id "CUFF.1.1"; exon_number "1"; FPKM "10679134.4063403048"; frac "1.000000"; conf_lo "8542701.791788"; conf_hi "12815567.020892"; cov "145.770185"; +test_chromosome Cufflinks exon 351 400 1000 + . gene_id "CUFF.1"; transcript_id "CUFF.1.1"; exon_number "2"; FPKM "10679134.4063403048"; frac "1.000000"; conf_lo "8542701.791788"; conf_hi "12815567.020892"; cov "145.770185"; +test_chromosome Cufflinks exon 501 550 1000 + . gene_id "CUFF.1"; transcript_id "CUFF.1.1"; exon_number "3"; FPKM "10679134.4063403048"; frac "1.000000"; conf_lo "8542701.791788"; conf_hi "12815567.020892"; cov "145.770185";
--- a/test-data/stringtie_out1.gtf Thu Jul 03 18:38:02 2014 -0400 +++ b/test-data/stringtie_out1.gtf Thu Mar 05 11:41:12 2015 -0500 @@ -1,154 +1,4 @@ -chr19 StringTie transcript 3052907 3062360 1000 - . gene_id "STRG.1"; transcript_id "STRG.1.1"; cov "883.073547";FPKM "189664.171875"; -chr19 StringTie exon 3052907 3054038 1000 - . gene_id "STRG.1"; transcript_id "STRG.1.1"; exon_number "1"; cov "966.177673"; -chr19 StringTie exon 3054118 3054192 1000 - . gene_id "STRG.1"; transcript_id "STRG.1.1"; exon_number "2"; cov "1301.127930"; -chr19 StringTie exon 3055662 3055724 1000 - . gene_id "STRG.1"; transcript_id "STRG.1.1"; exon_number "3"; cov "1990.478271"; -chr19 StringTie exon 3056310 3056354 1000 - . gene_id "STRG.1"; transcript_id "STRG.1.1"; exon_number "4"; cov "942.796082"; -chr19 StringTie exon 3057677 3057740 1000 - . gene_id "STRG.1"; transcript_id "STRG.1.1"; exon_number "5"; cov "351.962616"; -chr19 StringTie exon 3061158 3061255 1000 - . gene_id "STRG.1"; transcript_id "STRG.1.1"; exon_number "6"; cov "1122.192017"; -chr19 StringTie exon 3062172 3062360 1000 - . gene_id "STRG.1"; transcript_id "STRG.1.1"; exon_number "7"; cov "214.494049"; -chr19 StringTie transcript 3052907 3063089 1000 - . gene_id "STRG.1"; transcript_id "STRG.1.2"; cov "168.800812";FPKM "36254.585938"; -chr19 StringTie exon 3052907 3054038 1000 - . gene_id "STRG.1"; transcript_id "STRG.1.2"; exon_number "1"; cov "220.181381"; -chr19 StringTie exon 3054118 3054192 1000 - . gene_id "STRG.1"; transcript_id "STRG.1.2"; exon_number "2"; cov "258.932983"; -chr19 StringTie exon 3055662 3055724 1000 - . gene_id "STRG.1"; transcript_id "STRG.1.2"; exon_number "3"; cov "176.962967"; -chr19 StringTie exon 3056310 3056354 1000 - . gene_id "STRG.1"; transcript_id "STRG.1.2"; exon_number "4"; cov "118.451096"; -chr19 StringTie exon 3057677 3057740 1000 - . gene_id "STRG.1"; transcript_id "STRG.1.2"; exon_number "5"; cov "254.885681"; -chr19 StringTie exon 3061158 3061255 1000 - . gene_id "STRG.1"; transcript_id "STRG.1.2"; exon_number "6"; cov "78.436821"; -chr19 StringTie exon 3062698 3063089 1000 - . gene_id "STRG.1"; transcript_id "STRG.1.2"; exon_number "7"; cov "16.186066"; -chr19 StringTie transcript 2997664 3015854 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.1"; cov "115.851471";FPKM "24882.267578"; -chr19 StringTie exon 2997664 2997953 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.1"; exon_number "1"; cov "110.703667"; -chr19 StringTie exon 3000645 3000721 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.1"; exon_number "2"; cov "207.771072"; -chr19 StringTie exon 3002351 3002501 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.1"; exon_number "3"; cov "114.276024"; -chr19 StringTie exon 3005435 3005582 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.1"; exon_number "4"; cov "120.278976"; -chr19 StringTie exon 3005719 3005966 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.1"; exon_number "5"; cov "78.667053"; -chr19 StringTie exon 3006418 3006667 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.1"; exon_number "6"; cov "48.566441"; -chr19 StringTie exon 3008867 3008943 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.1"; exon_number "7"; cov "66.379005"; -chr19 StringTie exon 3009540 3009700 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.1"; exon_number "8"; cov "62.793331"; -chr19 StringTie exon 3011020 3011158 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.1"; exon_number "9"; cov "219.519562"; -chr19 StringTie exon 3013667 3013816 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.1"; exon_number "10"; cov "172.405579"; -chr19 StringTie exon 3014568 3014612 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.1"; exon_number "11"; cov "342.860687"; -chr19 StringTie exon 3015651 3015854 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.1"; exon_number "12"; cov "119.223740"; -chr19 StringTie transcript 3018960 3029141 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.2"; cov "18.253860";FPKM "3920.515381"; -chr19 StringTie exon 3018960 3019461 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.2"; exon_number "1"; cov "10.802196"; -chr19 StringTie exon 3019697 3019771 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.2"; exon_number "2"; cov "23.808632"; -chr19 StringTie exon 3025018 3025080 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.2"; exon_number "3"; cov "33.401360"; -chr19 StringTie exon 3027827 3027871 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.2"; exon_number "4"; cov "41.172310"; -chr19 StringTie exon 3028317 3028380 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.2"; exon_number "5"; cov "42.650341"; -chr19 StringTie exon 3028704 3028801 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.2"; exon_number "6"; cov "32.359097"; -chr19 StringTie exon 3028879 3029141 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.2"; exon_number "7"; cov "12.150498"; -chr19 StringTie transcript 3018960 3029141 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.3"; cov "5.218285";FPKM "1120.769287"; -chr19 StringTie exon 3018960 3019464 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.3"; exon_number "1"; cov "4.017776"; -chr19 StringTie exon 3019697 3019771 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.3"; exon_number "2"; cov "15.457561"; -chr19 StringTie exon 3025018 3025080 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.3"; exon_number "3"; cov "1.183362"; -chr19 StringTie exon 3027827 3027871 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.3"; exon_number "4"; cov "3.434842"; -chr19 StringTie exon 3028317 3028380 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.3"; exon_number "5"; cov "20.437429"; -chr19 StringTie exon 3028704 3028801 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.3"; exon_number "6"; cov "6.825138"; -chr19 StringTie exon 3028879 3029141 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.3"; exon_number "7"; cov "1.572921"; -chr19 StringTie transcript 3094613 3119121 1000 + . gene_id "STRG.3"; transcript_id "STRG.3.1"; cov "80.540161";FPKM "17298.201172"; -chr19 StringTie exon 3094613 3094785 1000 + . gene_id "STRG.3"; transcript_id "STRG.3.1"; exon_number "1"; cov "67.409538"; -chr19 StringTie exon 3110147 3110331 1000 + . gene_id "STRG.3"; transcript_id "STRG.3.1"; exon_number "2"; cov "102.204170"; -chr19 StringTie exon 3113328 3113482 1000 + . gene_id "STRG.3"; transcript_id "STRG.3.1"; exon_number "3"; cov "108.144516"; -chr19 StringTie exon 3114942 3115070 1000 + . gene_id "STRG.3"; transcript_id "STRG.3.1"; exon_number "4"; cov "63.896484"; -chr19 StringTie exon 3118922 3119121 1000 + . gene_id "STRG.3"; transcript_id "STRG.3.1"; exon_number "5"; cov "61.832138"; -chr19 StringTie transcript 3119186 3123995 1000 + . gene_id "STRG.4"; transcript_id "STRG.4.1"; cov "69.395134";FPKM "14904.501953"; -chr19 StringTie exon 3119186 3119357 1000 + . gene_id "STRG.4"; transcript_id "STRG.4.1"; exon_number "1"; cov "71.938263"; -chr19 StringTie exon 3120987 3123995 1000 + . gene_id "STRG.4"; transcript_id "STRG.4.1"; exon_number "2"; cov "69.249763"; -chr19 StringTie transcript 3136149 3163782 1000 + . gene_id "STRG.5"; transcript_id "STRG.5.1"; cov "13.414832";FPKM "2881.201660"; -chr19 StringTie exon 3136149 3136593 1000 + . gene_id "STRG.5"; transcript_id "STRG.5.1"; exon_number "1"; cov "2.243525"; -chr19 StringTie exon 3148589 3148773 1000 + . gene_id "STRG.5"; transcript_id "STRG.5.1"; exon_number "2"; cov "6.817567"; -chr19 StringTie exon 3150129 3150283 1000 + . gene_id "STRG.5"; transcript_id "STRG.5.1"; exon_number "3"; cov "12.169776"; -chr19 StringTie exon 3151705 3151833 1000 + . gene_id "STRG.5"; transcript_id "STRG.5.1"; exon_number "4"; cov "5.409668"; -chr19 StringTie exon 3155821 3155950 1000 + . gene_id "STRG.5"; transcript_id "STRG.5.1"; exon_number "5"; cov "22.124674"; -chr19 StringTie exon 3157726 3157879 1000 + . gene_id "STRG.5"; transcript_id "STRG.5.1"; exon_number "6"; cov "16.131796"; -chr19 StringTie exon 3162791 3163782 1000 + . gene_id "STRG.5"; transcript_id "STRG.5.1"; exon_number "7"; cov "19.328829"; -chr19 StringTie transcript 3176651 3177191 1000 . . gene_id "STRG.6"; transcript_id "STRG.6.1"; cov "2.218115";FPKM "476.400818"; -chr19 StringTie exon 3176651 3177191 1000 . . gene_id "STRG.6"; transcript_id "STRG.6.1"; exon_number "1"; cov "2.218115"; -chr19 StringTie transcript 3178008 3178218 1000 . . gene_id "STRG.7"; transcript_id "STRG.7.1"; cov "2.132701";FPKM "458.055786"; -chr19 StringTie exon 3178008 3178218 1000 . . gene_id "STRG.7"; transcript_id "STRG.7.1"; exon_number "1"; cov "2.132701"; -chr19 StringTie transcript 3178290 3182254 1000 . . gene_id "STRG.8"; transcript_id "STRG.8.1"; cov "25.143421";FPKM "5400.236816"; -chr19 StringTie exon 3178290 3182254 1000 . . gene_id "STRG.8"; transcript_id "STRG.8.1"; exon_number "1"; cov "25.143421"; -chr19 StringTie transcript 3182964 3183191 1000 . . gene_id "STRG.9"; transcript_id "STRG.9.1"; cov "3.289474";FPKM "706.504456"; -chr19 StringTie exon 3182964 3183191 1000 . . gene_id "STRG.9"; transcript_id "STRG.9.1"; exon_number "1"; cov "3.289474"; -chr19 StringTie transcript 3183534 3184980 1000 . . gene_id "STRG.10"; transcript_id "STRG.10.1"; cov "10.234969";FPKM "2198.239258"; -chr19 StringTie exon 3183534 3184980 1000 . . gene_id "STRG.10"; transcript_id "STRG.10.1"; exon_number "1"; cov "10.234969"; -chr19 StringTie transcript 3185059 3185762 1000 . . gene_id "STRG.11"; transcript_id "STRG.11.1"; cov "4.474432";FPKM "961.006531"; -chr19 StringTie exon 3185059 3185762 1000 . . gene_id "STRG.11"; transcript_id "STRG.11.1"; exon_number "1"; cov "4.474432"; -chr19 StringTie transcript 3185993 3209570 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.1"; cov "46.485111";FPKM "9983.947266"; -chr19 StringTie exon 3185993 3186212 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.1"; exon_number "1"; cov "9.429298"; -chr19 StringTie exon 3192468 3192658 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.1"; exon_number "2"; cov "21.059887"; -chr19 StringTie exon 3193282 3193426 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.1"; exon_number "3"; cov "39.715225"; -chr19 StringTie exon 3196181 3196275 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.1"; exon_number "4"; cov "37.911308"; -chr19 StringTie exon 3198815 3198895 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.1"; exon_number "5"; cov "51.996696"; -chr19 StringTie exon 3201521 3201624 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.1"; exon_number "6"; cov "31.073275"; -chr19 StringTie exon 3203754 3203842 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.1"; exon_number "7"; cov "55.852036"; -chr19 StringTie exon 3204003 3204142 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.1"; exon_number "8"; cov "30.771446"; -chr19 StringTie exon 3204571 3204749 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.1"; exon_number "9"; cov "34.595020"; -chr19 StringTie exon 3205937 3206024 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.1"; exon_number "10"; cov "43.030930"; -chr19 StringTie exon 3206150 3206188 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.1"; exon_number "11"; cov "59.276905"; -chr19 StringTie exon 3206260 3206423 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.1"; exon_number "12"; cov "46.387093"; -chr19 StringTie exon 3207196 3207249 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.1"; exon_number "13"; cov "63.118416"; -chr19 StringTie exon 3207389 3207467 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.1"; exon_number "14"; cov "65.674583"; -chr19 StringTie exon 3207627 3209570 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.1"; exon_number "15"; cov "54.248878"; -chr19 StringTie transcript 3195437 3209570 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.2"; cov "17.133051";FPKM "3679.790771"; -chr19 StringTie exon 3195437 3196275 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.2"; exon_number "1"; cov "10.562823"; -chr19 StringTie exon 3198815 3198895 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.2"; exon_number "2"; cov "17.045162"; -chr19 StringTie exon 3201521 3201624 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.2"; exon_number "3"; cov "9.639905"; -chr19 StringTie exon 3203754 3203842 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.2"; exon_number "4"; cov "14.507616"; -chr19 StringTie exon 3204003 3204142 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.2"; exon_number "5"; cov "5.000354"; -chr19 StringTie exon 3204571 3204749 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.2"; exon_number "6"; cov "10.209009"; -chr19 StringTie exon 3205937 3206024 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.2"; exon_number "7"; cov "16.365309"; -chr19 StringTie exon 3206150 3206188 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.2"; exon_number "8"; cov "25.572968"; -chr19 StringTie exon 3206263 3206423 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.2"; exon_number "9"; cov "19.185726"; -chr19 StringTie exon 3207196 3207249 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.2"; exon_number "10"; cov "38.189373"; -chr19 StringTie exon 3207389 3207467 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.2"; exon_number "11"; cov "31.688845"; -chr19 StringTie exon 3207627 3209570 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.2"; exon_number "12"; cov "20.523384"; -chr19 StringTie transcript 3203443 3209570 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.3"; cov "7.056122";FPKM "1515.494995"; -chr19 StringTie exon 3203443 3203842 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.3"; exon_number "1"; cov "2.242009"; -chr19 StringTie exon 3204003 3204142 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.3"; exon_number "2"; cov "3.895410"; -chr19 StringTie exon 3204571 3204749 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.3"; exon_number "3"; cov "6.615313"; -chr19 StringTie exon 3205937 3206024 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.3"; exon_number "4"; cov "10.143439"; -chr19 StringTie exon 3206150 3206188 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.3"; exon_number "5"; cov "11.954673"; -chr19 StringTie exon 3206260 3207249 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.3"; exon_number "6"; cov "3.642324"; -chr19 StringTie exon 3207389 3207467 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.3"; exon_number "7"; cov "5.884272"; -chr19 StringTie exon 3207627 3209570 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.3"; exon_number "8"; cov "9.862993"; -chr19 StringTie transcript 3363620 3364229 1000 . . gene_id "STRG.13"; transcript_id "STRG.13.1"; cov "2.622951";FPKM "563.350403"; -chr19 StringTie exon 3363620 3364229 1000 . . gene_id "STRG.13"; transcript_id "STRG.13.1"; exon_number "1"; cov "2.622951"; -chr19 StringTie transcript 3366538 3469274 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.1"; cov "71.175430";FPKM "15286.868164"; -chr19 StringTie exon 3366538 3366664 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.1"; exon_number "1"; cov "11.686789"; -chr19 StringTie exon 3381710 3382241 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.1"; exon_number "2"; cov "56.353500"; -chr19 StringTie exon 3425104 3425175 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.1"; exon_number "3"; cov "74.173615"; -chr19 StringTie exon 3433516 3433590 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.1"; exon_number "4"; cov "65.354446"; -chr19 StringTie exon 3434275 3434398 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.1"; exon_number "5"; cov "80.448212"; -chr19 StringTie exon 3435081 3435205 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.1"; exon_number "6"; cov "71.471077"; -chr19 StringTie exon 3449012 3449137 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.1"; exon_number "7"; cov "107.203087"; -chr19 StringTie exon 3452480 3452664 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.1"; exon_number "8"; cov "71.914742"; -chr19 StringTie exon 3453761 3453914 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.1"; exon_number "9"; cov "30.357872"; -chr19 StringTie exon 3456548 3456633 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.1"; exon_number "10"; cov "40.561024"; -chr19 StringTie exon 3462750 3469274 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.1"; exon_number "11"; cov "74.051750"; -chr19 StringTie transcript 3366538 3469274 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.2"; cov "18.338432";FPKM "3938.679688"; -chr19 StringTie exon 3366538 3366664 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.2"; exon_number "1"; cov "3.412646"; -chr19 StringTie exon 3381710 3382241 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.2"; exon_number "2"; cov "15.545184"; -chr19 StringTie exon 3425104 3425175 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.2"; exon_number "3"; cov "21.998638"; -chr19 StringTie exon 3433516 3433590 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.2"; exon_number "4"; cov "17.916565"; -chr19 StringTie exon 3434275 3434398 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.2"; exon_number "5"; cov "24.749645"; -chr19 StringTie exon 3435081 3435205 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.2"; exon_number "6"; cov "20.192204"; -chr19 StringTie exon 3449012 3449137 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.2"; exon_number "7"; cov "19.043257"; -chr19 StringTie exon 3452480 3452664 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.2"; exon_number "8"; cov "32.998787"; -chr19 StringTie exon 3462750 3469274 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.2"; exon_number "9"; cov "18.234524"; -chr19 StringTie transcript 3472911 3473297 1000 . . gene_id "STRG.15"; transcript_id "STRG.15.1"; cov "2.842377";FPKM "610.478088"; -chr19 StringTie exon 3472911 3473297 1000 . . gene_id "STRG.15"; transcript_id "STRG.15.1"; exon_number "1"; cov "2.842377"; -chr19 StringTie transcript 3473383 3473585 1000 . . gene_id "STRG.16"; transcript_id "STRG.16.1"; cov "2.216749";FPKM "476.107422"; -chr19 StringTie exon 3473383 3473585 1000 . . gene_id "STRG.16"; transcript_id "STRG.16.1"; exon_number "1"; cov "2.216749"; -chr19 StringTie transcript 3473881 3474130 1000 . . gene_id "STRG.17"; transcript_id "STRG.17.1"; cov "5.800000";FPKM "1245.708496"; -chr19 StringTie exon 3473881 3474130 1000 . . gene_id "STRG.17"; transcript_id "STRG.17.1"; exon_number "1"; cov "5.800000"; -chr19 StringTie transcript 3474201 3474891 1000 . . gene_id "STRG.18"; transcript_id "STRG.18.1"; cov "6.512301";FPKM "1398.694580"; -chr19 StringTie exon 3474201 3474891 1000 . . gene_id "STRG.18"; transcript_id "STRG.18.1"; exon_number "1"; cov "6.512301"; -chr19 StringTie transcript 3475786 3476529 1000 . . gene_id "STRG.19"; transcript_id "STRG.19.1"; cov "4.166667";FPKM "894.905579"; -chr19 StringTie exon 3475786 3476529 1000 . . gene_id "STRG.19"; transcript_id "STRG.19.1"; exon_number "1"; cov "4.166667"; -chr19 StringTie transcript 3474957 3475188 1000 - . gene_id "STRG.20"; transcript_id "STRG.20.1"; cov "3.719828";FPKM "798.934692"; -chr19 StringTie exon 3474957 3475188 1000 - . gene_id "STRG.20"; transcript_id "STRG.20.1"; exon_number "1"; cov "3.719828"; -chr19 StringTie transcript 3490820 3500661 1000 - . gene_id "STRG.21"; transcript_id "STRG.21.1"; cov "38.885868";FPKM "8351.802734"; -chr19 StringTie exon 3490820 3491809 1000 - . gene_id "STRG.21"; transcript_id "STRG.21.1"; exon_number "1"; cov "49.893391"; -chr19 StringTie exon 3492260 3492497 1000 - . gene_id "STRG.21"; transcript_id "STRG.21.1"; exon_number "2"; cov "14.960925"; -chr19 StringTie exon 3494026 3494102 1000 - . gene_id "STRG.21"; transcript_id "STRG.21.1"; exon_number "3"; cov "22.457817"; -chr19 StringTie exon 3496539 3496884 1000 - . gene_id "STRG.21"; transcript_id "STRG.21.1"; exon_number "4"; cov "35.878857"; -chr19 StringTie exon 3500559 3500661 1000 - . gene_id "STRG.21"; transcript_id "STRG.21.1"; exon_number "5"; cov "10.650763"; +test_chromosome StringTie transcript 53 550 1000 + . gene_id "STRG.1"; transcript_id "STRG.1.1"; cov "44.724823";FPKM "3276543.750000"; +test_chromosome StringTie exon 53 250 1000 + . gene_id "STRG.1"; transcript_id "STRG.1.1"; exon_number "1"; cov "49.011967"; +test_chromosome StringTie exon 351 400 1000 + . gene_id "STRG.1"; transcript_id "STRG.1.1"; exon_number "2"; cov "51.382565"; +test_chromosome StringTie exon 501 550 1000 + . gene_id "STRG.1"; transcript_id "STRG.1.1"; exon_number "3"; cov "21.090000";
--- a/test-data/stringtie_out2.gtf Thu Jul 03 18:38:02 2014 -0400 +++ b/test-data/stringtie_out2.gtf Thu Mar 05 11:41:12 2015 -0500 @@ -1,145 +1,4 @@ -chr19 StringTie transcript 3052907 3062360 1000 - . gene_id "STRG.1"; transcript_id "STRG.1.1"; cov "883.073547";FPKM "189664.171875"; -chr19 StringTie exon 3052907 3054038 1000 - . gene_id "STRG.1"; transcript_id "STRG.1.1"; exon_number "1"; cov "966.177673"; -chr19 StringTie exon 3054118 3054192 1000 - . gene_id "STRG.1"; transcript_id "STRG.1.1"; exon_number "2"; cov "1301.127930"; -chr19 StringTie exon 3055662 3055724 1000 - . gene_id "STRG.1"; transcript_id "STRG.1.1"; exon_number "3"; cov "1990.478271"; -chr19 StringTie exon 3056310 3056354 1000 - . gene_id "STRG.1"; transcript_id "STRG.1.1"; exon_number "4"; cov "942.796082"; -chr19 StringTie exon 3057677 3057740 1000 - . gene_id "STRG.1"; transcript_id "STRG.1.1"; exon_number "5"; cov "351.962616"; -chr19 StringTie exon 3061158 3061255 1000 - . gene_id "STRG.1"; transcript_id "STRG.1.1"; exon_number "6"; cov "1122.192017"; -chr19 StringTie exon 3062172 3062360 1000 - . gene_id "STRG.1"; transcript_id "STRG.1.1"; exon_number "7"; cov "214.494049"; -chr19 StringTie transcript 3052907 3063089 1000 - . gene_id "STRG.1"; transcript_id "STRG.1.2"; cov "168.800812";FPKM "36254.585938"; -chr19 StringTie exon 3052907 3054038 1000 - . gene_id "STRG.1"; transcript_id "STRG.1.2"; exon_number "1"; cov "220.181381"; -chr19 StringTie exon 3054118 3054192 1000 - . gene_id "STRG.1"; transcript_id "STRG.1.2"; exon_number "2"; cov "258.932983"; -chr19 StringTie exon 3055662 3055724 1000 - . gene_id "STRG.1"; transcript_id "STRG.1.2"; exon_number "3"; cov "176.962967"; -chr19 StringTie exon 3056310 3056354 1000 - . gene_id "STRG.1"; transcript_id "STRG.1.2"; exon_number "4"; cov "118.451096"; -chr19 StringTie exon 3057677 3057740 1000 - . gene_id "STRG.1"; transcript_id "STRG.1.2"; exon_number "5"; cov "254.885681"; -chr19 StringTie exon 3061158 3061255 1000 - . gene_id "STRG.1"; transcript_id "STRG.1.2"; exon_number "6"; cov "78.436821"; -chr19 StringTie exon 3062698 3063089 1000 - . gene_id "STRG.1"; transcript_id "STRG.1.2"; exon_number "7"; cov "16.186066"; -chr19 StringTie transcript 2997664 3015854 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.1"; cov "115.851471";FPKM "24882.267578"; -chr19 StringTie exon 2997664 2997953 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.1"; exon_number "1"; cov "110.703667"; -chr19 StringTie exon 3000645 3000721 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.1"; exon_number "2"; cov "207.771072"; -chr19 StringTie exon 3002351 3002501 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.1"; exon_number "3"; cov "114.276024"; -chr19 StringTie exon 3005435 3005582 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.1"; exon_number "4"; cov "120.278976"; -chr19 StringTie exon 3005719 3005966 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.1"; exon_number "5"; cov "78.667053"; -chr19 StringTie exon 3006418 3006667 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.1"; exon_number "6"; cov "48.566441"; -chr19 StringTie exon 3008867 3008943 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.1"; exon_number "7"; cov "66.379005"; -chr19 StringTie exon 3009540 3009700 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.1"; exon_number "8"; cov "62.793331"; -chr19 StringTie exon 3011020 3011158 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.1"; exon_number "9"; cov "219.519562"; -chr19 StringTie exon 3013667 3013816 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.1"; exon_number "10"; cov "172.405579"; -chr19 StringTie exon 3014568 3014612 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.1"; exon_number "11"; cov "342.860687"; -chr19 StringTie exon 3015651 3015854 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.1"; exon_number "12"; cov "119.223740"; -chr19 StringTie transcript 3018960 3029141 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.2"; cov "18.253860";FPKM "3920.515381"; -chr19 StringTie exon 3018960 3019461 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.2"; exon_number "1"; cov "10.802196"; -chr19 StringTie exon 3019697 3019771 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.2"; exon_number "2"; cov "23.808632"; -chr19 StringTie exon 3025018 3025080 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.2"; exon_number "3"; cov "33.401360"; -chr19 StringTie exon 3027827 3027871 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.2"; exon_number "4"; cov "41.172310"; -chr19 StringTie exon 3028317 3028380 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.2"; exon_number "5"; cov "42.650341"; -chr19 StringTie exon 3028704 3028801 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.2"; exon_number "6"; cov "32.359097"; -chr19 StringTie exon 3028879 3029141 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.2"; exon_number "7"; cov "12.150498"; -chr19 StringTie transcript 3018960 3029141 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.3"; cov "5.218285";FPKM "1120.769287"; -chr19 StringTie exon 3018960 3019464 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.3"; exon_number "1"; cov "4.017776"; -chr19 StringTie exon 3019697 3019771 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.3"; exon_number "2"; cov "15.457561"; -chr19 StringTie exon 3025018 3025080 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.3"; exon_number "3"; cov "1.183362"; -chr19 StringTie exon 3027827 3027871 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.3"; exon_number "4"; cov "3.434842"; -chr19 StringTie exon 3028317 3028380 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.3"; exon_number "5"; cov "20.437429"; -chr19 StringTie exon 3028704 3028801 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.3"; exon_number "6"; cov "6.825138"; -chr19 StringTie exon 3028879 3029141 1000 - . gene_id "STRG.2"; transcript_id "STRG.2.3"; exon_number "7"; cov "1.572921"; -chr19 StringTie transcript 3094613 3119121 1000 + . gene_id "STRG.3"; transcript_id "STRG.3.1"; cov "80.540161";FPKM "17298.201172"; -chr19 StringTie exon 3094613 3094785 1000 + . gene_id "STRG.3"; transcript_id "STRG.3.1"; exon_number "1"; cov "67.409538"; -chr19 StringTie exon 3110147 3110331 1000 + . gene_id "STRG.3"; transcript_id "STRG.3.1"; exon_number "2"; cov "102.204170"; -chr19 StringTie exon 3113328 3113482 1000 + . gene_id "STRG.3"; transcript_id "STRG.3.1"; exon_number "3"; cov "108.144516"; -chr19 StringTie exon 3114942 3115070 1000 + . gene_id "STRG.3"; transcript_id "STRG.3.1"; exon_number "4"; cov "63.896484"; -chr19 StringTie exon 3118922 3119121 1000 + . gene_id "STRG.3"; transcript_id "STRG.3.1"; exon_number "5"; cov "61.832138"; -chr19 StringTie transcript 3119186 3123995 1000 + . gene_id "STRG.4"; transcript_id "STRG.4.1"; cov "69.395134";FPKM "14904.501953"; -chr19 StringTie exon 3119186 3119357 1000 + . gene_id "STRG.4"; transcript_id "STRG.4.1"; exon_number "1"; cov "71.938263"; -chr19 StringTie exon 3120987 3123995 1000 + . gene_id "STRG.4"; transcript_id "STRG.4.1"; exon_number "2"; cov "69.249763"; -chr19 StringTie transcript 3136149 3163782 1000 + . gene_id "STRG.5"; transcript_id "STRG.5.1"; cov "13.414832";FPKM "2881.201660"; -chr19 StringTie exon 3136149 3136593 1000 + . gene_id "STRG.5"; transcript_id "STRG.5.1"; exon_number "1"; cov "2.243525"; -chr19 StringTie exon 3148589 3148773 1000 + . gene_id "STRG.5"; transcript_id "STRG.5.1"; exon_number "2"; cov "6.817567"; -chr19 StringTie exon 3150129 3150283 1000 + . gene_id "STRG.5"; transcript_id "STRG.5.1"; exon_number "3"; cov "12.169776"; -chr19 StringTie exon 3151705 3151833 1000 + . gene_id "STRG.5"; transcript_id "STRG.5.1"; exon_number "4"; cov "5.409668"; -chr19 StringTie exon 3155821 3155950 1000 + . gene_id "STRG.5"; transcript_id "STRG.5.1"; exon_number "5"; cov "22.124674"; -chr19 StringTie exon 3157726 3157879 1000 + . gene_id "STRG.5"; transcript_id "STRG.5.1"; exon_number "6"; cov "16.131796"; -chr19 StringTie exon 3162791 3163782 1000 + . gene_id "STRG.5"; transcript_id "STRG.5.1"; exon_number "7"; cov "19.328829"; -chr19 StringTie transcript 3176651 3177191 1000 . . gene_id "STRG.6"; transcript_id "STRG.6.1"; cov "2.218115";FPKM "476.400818"; -chr19 StringTie exon 3176651 3177191 1000 . . gene_id "STRG.6"; transcript_id "STRG.6.1"; exon_number "1"; cov "2.218115"; -chr19 StringTie transcript 3178008 3178218 1000 . . gene_id "STRG.7"; transcript_id "STRG.7.1"; cov "2.132701";FPKM "458.055786"; -chr19 StringTie exon 3178008 3178218 1000 . . gene_id "STRG.7"; transcript_id "STRG.7.1"; exon_number "1"; cov "2.132701"; -chr19 StringTie transcript 3178290 3182254 1000 . . gene_id "STRG.8"; transcript_id "STRG.8.1"; cov "25.143421";FPKM "5400.236816"; -chr19 StringTie exon 3178290 3182254 1000 . . gene_id "STRG.8"; transcript_id "STRG.8.1"; exon_number "1"; cov "25.143421"; -chr19 StringTie transcript 3182964 3183191 1000 . . gene_id "STRG.9"; transcript_id "STRG.9.1"; cov "3.289474";FPKM "706.504456"; -chr19 StringTie exon 3182964 3183191 1000 . . gene_id "STRG.9"; transcript_id "STRG.9.1"; exon_number "1"; cov "3.289474"; -chr19 StringTie transcript 3183534 3184980 1000 . . gene_id "STRG.10"; transcript_id "STRG.10.1"; cov "10.234969";FPKM "2198.239258"; -chr19 StringTie exon 3183534 3184980 1000 . . gene_id "STRG.10"; transcript_id "STRG.10.1"; exon_number "1"; cov "10.234969"; -chr19 StringTie transcript 3185059 3185762 1000 . . gene_id "STRG.11"; transcript_id "STRG.11.1"; cov "4.474432";FPKM "961.006531"; -chr19 StringTie exon 3185059 3185762 1000 . . gene_id "STRG.11"; transcript_id "STRG.11.1"; exon_number "1"; cov "4.474432"; -chr19 StringTie transcript 3185993 3209570 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.1"; cov "46.485111";FPKM "9983.947266"; -chr19 StringTie exon 3185993 3186212 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.1"; exon_number "1"; cov "9.429298"; -chr19 StringTie exon 3192468 3192658 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.1"; exon_number "2"; cov "21.059887"; -chr19 StringTie exon 3193282 3193426 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.1"; exon_number "3"; cov "39.715225"; -chr19 StringTie exon 3196181 3196275 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.1"; exon_number "4"; cov "37.911308"; -chr19 StringTie exon 3198815 3198895 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.1"; exon_number "5"; cov "51.996696"; -chr19 StringTie exon 3201521 3201624 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.1"; exon_number "6"; cov "31.073275"; -chr19 StringTie exon 3203754 3203842 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.1"; exon_number "7"; cov "55.852036"; -chr19 StringTie exon 3204003 3204142 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.1"; exon_number "8"; cov "30.771446"; -chr19 StringTie exon 3204571 3204749 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.1"; exon_number "9"; cov "34.595020"; -chr19 StringTie exon 3205937 3206024 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.1"; exon_number "10"; cov "43.030930"; -chr19 StringTie exon 3206150 3206188 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.1"; exon_number "11"; cov "59.276905"; -chr19 StringTie exon 3206260 3206423 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.1"; exon_number "12"; cov "46.387093"; -chr19 StringTie exon 3207196 3207249 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.1"; exon_number "13"; cov "63.118416"; -chr19 StringTie exon 3207389 3207467 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.1"; exon_number "14"; cov "65.674583"; -chr19 StringTie exon 3207627 3209570 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.1"; exon_number "15"; cov "54.248878"; -chr19 StringTie transcript 3195437 3209570 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.2"; cov "17.133051";FPKM "3679.790771"; -chr19 StringTie exon 3195437 3196275 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.2"; exon_number "1"; cov "10.562823"; -chr19 StringTie exon 3198815 3198895 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.2"; exon_number "2"; cov "17.045162"; -chr19 StringTie exon 3201521 3201624 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.2"; exon_number "3"; cov "9.639905"; -chr19 StringTie exon 3203754 3203842 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.2"; exon_number "4"; cov "14.507616"; -chr19 StringTie exon 3204003 3204142 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.2"; exon_number "5"; cov "5.000354"; -chr19 StringTie exon 3204571 3204749 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.2"; exon_number "6"; cov "10.209009"; -chr19 StringTie exon 3205937 3206024 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.2"; exon_number "7"; cov "16.365309"; -chr19 StringTie exon 3206150 3206188 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.2"; exon_number "8"; cov "25.572968"; -chr19 StringTie exon 3206263 3206423 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.2"; exon_number "9"; cov "19.185726"; -chr19 StringTie exon 3207196 3207249 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.2"; exon_number "10"; cov "38.189373"; -chr19 StringTie exon 3207389 3207467 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.2"; exon_number "11"; cov "31.688845"; -chr19 StringTie exon 3207627 3209570 1000 + . gene_id "STRG.12"; transcript_id "STRG.12.2"; exon_number "12"; cov "20.523384"; -chr19 StringTie transcript 3363620 3364229 1000 . . gene_id "STRG.13"; transcript_id "STRG.13.1"; cov "2.622951";FPKM "563.350403"; -chr19 StringTie exon 3363620 3364229 1000 . . gene_id "STRG.13"; transcript_id "STRG.13.1"; exon_number "1"; cov "2.622951"; -chr19 StringTie transcript 3366538 3469274 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.1"; cov "71.175430";FPKM "15286.868164"; -chr19 StringTie exon 3366538 3366664 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.1"; exon_number "1"; cov "11.686789"; -chr19 StringTie exon 3381710 3382241 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.1"; exon_number "2"; cov "56.353500"; -chr19 StringTie exon 3425104 3425175 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.1"; exon_number "3"; cov "74.173615"; -chr19 StringTie exon 3433516 3433590 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.1"; exon_number "4"; cov "65.354446"; -chr19 StringTie exon 3434275 3434398 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.1"; exon_number "5"; cov "80.448212"; -chr19 StringTie exon 3435081 3435205 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.1"; exon_number "6"; cov "71.471077"; -chr19 StringTie exon 3449012 3449137 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.1"; exon_number "7"; cov "107.203087"; -chr19 StringTie exon 3452480 3452664 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.1"; exon_number "8"; cov "71.914742"; -chr19 StringTie exon 3453761 3453914 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.1"; exon_number "9"; cov "30.357872"; -chr19 StringTie exon 3456548 3456633 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.1"; exon_number "10"; cov "40.561024"; -chr19 StringTie exon 3462750 3469274 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.1"; exon_number "11"; cov "74.051750"; -chr19 StringTie transcript 3366538 3469274 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.2"; cov "18.338432";FPKM "3938.679688"; -chr19 StringTie exon 3366538 3366664 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.2"; exon_number "1"; cov "3.412646"; -chr19 StringTie exon 3381710 3382241 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.2"; exon_number "2"; cov "15.545184"; -chr19 StringTie exon 3425104 3425175 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.2"; exon_number "3"; cov "21.998638"; -chr19 StringTie exon 3433516 3433590 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.2"; exon_number "4"; cov "17.916565"; -chr19 StringTie exon 3434275 3434398 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.2"; exon_number "5"; cov "24.749645"; -chr19 StringTie exon 3435081 3435205 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.2"; exon_number "6"; cov "20.192204"; -chr19 StringTie exon 3449012 3449137 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.2"; exon_number "7"; cov "19.043257"; -chr19 StringTie exon 3452480 3452664 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.2"; exon_number "8"; cov "32.998787"; -chr19 StringTie exon 3462750 3469274 1000 + . gene_id "STRG.14"; transcript_id "STRG.14.2"; exon_number "9"; cov "18.234524"; -chr19 StringTie transcript 3472911 3473297 1000 . . gene_id "STRG.15"; transcript_id "STRG.15.1"; cov "2.842377";FPKM "610.478088"; -chr19 StringTie exon 3472911 3473297 1000 . . gene_id "STRG.15"; transcript_id "STRG.15.1"; exon_number "1"; cov "2.842377"; -chr19 StringTie transcript 3473383 3473585 1000 . . gene_id "STRG.16"; transcript_id "STRG.16.1"; cov "2.216749";FPKM "476.107422"; -chr19 StringTie exon 3473383 3473585 1000 . . gene_id "STRG.16"; transcript_id "STRG.16.1"; exon_number "1"; cov "2.216749"; -chr19 StringTie transcript 3473881 3474130 1000 . . gene_id "STRG.17"; transcript_id "STRG.17.1"; cov "5.800000";FPKM "1245.708496"; -chr19 StringTie exon 3473881 3474130 1000 . . gene_id "STRG.17"; transcript_id "STRG.17.1"; exon_number "1"; cov "5.800000"; -chr19 StringTie transcript 3474201 3474891 1000 . . gene_id "STRG.18"; transcript_id "STRG.18.1"; cov "6.512301";FPKM "1398.694580"; -chr19 StringTie exon 3474201 3474891 1000 . . gene_id "STRG.18"; transcript_id "STRG.18.1"; exon_number "1"; cov "6.512301"; -chr19 StringTie transcript 3475786 3476529 1000 . . gene_id "STRG.19"; transcript_id "STRG.19.1"; cov "4.166667";FPKM "894.905579"; -chr19 StringTie exon 3475786 3476529 1000 . . gene_id "STRG.19"; transcript_id "STRG.19.1"; exon_number "1"; cov "4.166667"; -chr19 StringTie transcript 3474957 3475188 1000 - . gene_id "STRG.20"; transcript_id "STRG.20.1"; cov "3.719828";FPKM "798.934692"; -chr19 StringTie exon 3474957 3475188 1000 - . gene_id "STRG.20"; transcript_id "STRG.20.1"; exon_number "1"; cov "3.719828"; -chr19 StringTie transcript 3490820 3500661 1000 - . gene_id "STRG.21"; transcript_id "STRG.21.1"; cov "38.885868";FPKM "8351.802734"; -chr19 StringTie exon 3490820 3491809 1000 - . gene_id "STRG.21"; transcript_id "STRG.21.1"; exon_number "1"; cov "49.893391"; -chr19 StringTie exon 3492260 3492497 1000 - . gene_id "STRG.21"; transcript_id "STRG.21.1"; exon_number "2"; cov "14.960925"; -chr19 StringTie exon 3494026 3494102 1000 - . gene_id "STRG.21"; transcript_id "STRG.21.1"; exon_number "3"; cov "22.457817"; -chr19 StringTie exon 3496539 3496884 1000 - . gene_id "STRG.21"; transcript_id "STRG.21.1"; exon_number "4"; cov "35.878857"; -chr19 StringTie exon 3500559 3500661 1000 - . gene_id "STRG.21"; transcript_id "STRG.21.1"; exon_number "5"; cov "10.650763"; +test_chromosome StringTie transcript 53 550 1000 + . gene_id "STRG.1"; transcript_id "STRG.1.1"; cov "44.724823";FPKM "3276543.750000"; +test_chromosome StringTie exon 53 250 1000 + . gene_id "STRG.1"; transcript_id "STRG.1.1"; exon_number "1"; cov "49.011967"; +test_chromosome StringTie exon 351 400 1000 + . gene_id "STRG.1"; transcript_id "STRG.1.1"; exon_number "2"; cov "51.382565"; +test_chromosome StringTie exon 501 550 1000 + . gene_id "STRG.1"; transcript_id "STRG.1.1"; exon_number "3"; cov "21.090000";
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/stringtie_out3.gtf Thu Mar 05 11:41:12 2015 -0500 @@ -0,0 +1,4 @@ +test_chromosome StringTie transcript 53 550 1000 + . gene_id "STRG.1"; transcript_id "STRG.1.1"; reference_id "CUFF.1.1"; cov "44.724823";FPKM "3276543.750000"; +test_chromosome StringTie exon 53 250 1000 + . gene_id "STRG.1"; transcript_id "STRG.1.1"; exon_number "1"; reference_id "CUFF.1.1"; cov "49.011967"; +test_chromosome StringTie exon 351 400 1000 + . gene_id "STRG.1"; transcript_id "STRG.1.1"; exon_number "2"; reference_id "CUFF.1.1"; cov "51.382565"; +test_chromosome StringTie exon 501 550 1000 + . gene_id "STRG.1"; transcript_id "STRG.1.1"; exon_number "3"; reference_id "CUFF.1.1"; cov "21.090000";
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/stringtie_out4.gtf Thu Mar 05 11:41:12 2015 -0500 @@ -0,0 +1,4 @@ +test_chromosome StringTie transcript 53 550 1000 + . gene_id "STRG.1"; transcript_id "STRG.1.1"; reference_id "CUFF.1.1"; cov "44.724823";FPKM "3276543.750000"; +test_chromosome StringTie exon 53 250 1000 + . gene_id "STRG.1"; transcript_id "STRG.1.1"; exon_number "1"; reference_id "CUFF.1.1"; cov "49.011967"; +test_chromosome StringTie exon 351 400 1000 + . gene_id "STRG.1"; transcript_id "STRG.1.1"; exon_number "2"; reference_id "CUFF.1.1"; cov "51.382565"; +test_chromosome StringTie exon 501 550 1000 + . gene_id "STRG.1"; transcript_id "STRG.1.1"; exon_number "3"; reference_id "CUFF.1.1"; cov "21.090000";
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/stringtie_out5.gtf Thu Mar 05 11:41:12 2015 -0500 @@ -0,0 +1,4 @@ +test_chromosome StringTie transcript 53 550 1000 + . gene_id "STRG.1"; transcript_id "STRG.1.1"; reference_id "CUFF.1.1"; cov "44.724823";FPKM "3276543.750000"; +test_chromosome StringTie exon 53 250 1000 + . gene_id "STRG.1"; transcript_id "STRG.1.1"; exon_number "1"; reference_id "CUFF.1.1"; cov "49.011967"; +test_chromosome StringTie exon 351 400 1000 + . gene_id "STRG.1"; transcript_id "STRG.1.1"; exon_number "2"; reference_id "CUFF.1.1"; cov "51.382565"; +test_chromosome StringTie exon 501 550 1000 + . gene_id "STRG.1"; transcript_id "STRG.1.1"; exon_number "3"; reference_id "CUFF.1.1"; cov "21.090000";
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/stringtie_out_coverage.gtf Thu Mar 05 11:41:12 2015 -0500 @@ -0,0 +1,4 @@ +test_chromosome Cufflinks transcript 53 550 1000.00 + . ID=CUFF.1.1;geneID=CUFF.1 +test_chromosome Cufflinks exon 53 250 1000.00 + . Parent=CUFF.1.1 +test_chromosome Cufflinks exon 351 400 1000.00 + . Parent=CUFF.1.1 +test_chromosome Cufflinks exon 501 550 1000.00 + . Parent=CUFF.1.1
--- a/tool_dependencies.xml Thu Jul 03 18:38:02 2014 -0400 +++ b/tool_dependencies.xml Thu Mar 05 11:41:12 2015 -0500 @@ -1,6 +1,6 @@ <?xml version="1.0"?> <tool_dependency> - <package name="stringtie" version="0.97"> - <repository changeset_revision="bbcdfb50d3f0" name="package_stringtie_0_97" owner="iuc" toolshed="http://toolshed.g2.bx.psu.edu" /> + <package name="stringtie" version="1.0.1"> + <repository changeset_revision="cd94bd10219b" name="package_stringtie_1_0_1" owner="iuc" toolshed="https://toolshed.g2.bx.psu.edu" /> </package> </tool_dependency>