# HG changeset patch
# User iuc
# Date 1634048466 0
# Node ID 6ec40b104f9d2230e0cc9b32a612ea0e2f305296

"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/tracy commit 8a1c82789c6ef97008ecf8f55e060422fd72f217"

diff -r 000000000000 -r 6ec40b104f9d macros.xml
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/macros.xml	Tue Oct 12 14:21:06 2021 +0000
@@ -0,0 +1,79 @@
+<macros>
+    <token name="@TOOL_VERSION@">0.6.1</token>
+    <xml name="requirements">
+        <requirements>
+            <requirement type="package" version="@TOOL_VERSION@">tracy</requirement>
+            <yield/>
+        </requirements>
+    </xml>
+    <xml name="citations">
+        <citations>
+            <citation type="doi">10.1186/s12864-020-6635-8</citation>
+            <yield />
+        </citations>
+    </xml>
+    <xml name="version_command">
+        <version_command>tracy --version</version_command>
+    </xml>
+    <xml name="common_options">
+        <param argument="--pratio" type="float" value="0.33" label="Peak ratio to call base" />
+        <param argument="--maxindel" type="integer" value="1000" label="Maximum indel size in Sanger trace" min="1" />
+        <param argument="--linelimit" type="integer" value="60" label="Alignment line length" min="1" />
+    </xml>
+    <xml name="kmer_options">
+        <section name="kmer" title="Kmer options">
+            <param argument="--kmer" type="integer" value="15" label="Kmer size to anchor trace" min="1" />
+            <param argument="--support" type="integer" value="3" label="Minimum kmer support" min="1" />
+        </section>
+    </xml>
+    <xml name="trim_options">
+        <section name="trim" title="Trimming options">
+            <param argument="--trim" type="integer" value="0" label="Trimming stringency (0: use trimLeft and trimRight)" />
+            <param argument="--trimLeft" type="integer" value="50" label="Trim size left" min="0" />
+            <param argument="--trimRight" type="integer" value="50" label="Trim size right" min="0" />
+        </section>
+    </xml>
+    <xml name="alignment_options">
+        <section name="alignment" title="Alignment options">
+            <param argument="--gapopen" type="integer" value="-10" max="0" label="Gap open penalty" />
+            <param argument="--gapext" type="integer" value="-4" max="0" label="Gap extension penalty" />
+            <param argument="--match" type="integer" value="3" min="0" label="Match score" />
+            <param argument="--mismatch" type="integer" value="-5" max="0" label="Mismatch penalty" />
+        </section>
+    </xml>
+    <token name="@pratio@"><![CDATA[
+The *pratio* parameter is a floating point value expressing the minimum proportion of the maximum trace value that will still be
+called as a base. For example, if the maximum value found in the trace file is 10, and pratio is 0.3, a peak needs to be
+higher than 3 to be called as a base.        
+    ]]></token>
+    <token name="@trim_options@"><![CDATA[
+Prior to alignment the trace file is trimmed. Either a fixed number of bases can be trimmed from the left or right sides of
+the trace, or trimming points (on the left or right or the trace) can be automatically found using an algorithm that works
+outwards from the best 10% of the trace and uses a sliding window (of size 10 bases) to find the point where the trace
+quality falls below a computed threshold. This automated trimming algorithm can be tuned using a *trimming stringency* (higher
+values mean stricter trimming).
+    ]]></token>
+    <token name="@alignment@"><![CDATA[
+The dynamic programming step of the alignment uses the `Gotoh's algorithm <http://rna.informatik.uni-freiburg.de/Teaching/index.jsp?toolName=Gotoh>`_ 
+which can be tuned using scores for nucleotide matches, mismatches, gap opening and gap extension.
+    ]]></token>
+    <xml name="optional_outputs">
+        <param name="optional_outputs" type="select" multiple="true" label="Optional outputs">
+            <option value="json">JSON</option>
+            <option value="tabular">tabular statistics report</option>
+        </param>
+    </xml>
+    <xml name="json_output" token_toolname="">
+        <data name="out_json" format="json" from_work_dir="out.json" label="tracy @TOOLNAME@ JSON on ${on_string}">
+            <filter>optional_outputs and 'json' in optional_outputs</filter>
+        </data>    
+    </xml>
+    <xml name="tabular_output" token_toolname="">
+        <data name="out_stats" format="tabular" from_work_dir="out.abif" label="tracy @TOOLNAME@ stats on ${on_string}">
+            <actions>
+                <action name="column_names" type="metadata" default="pos,peakA,peakC,peakG,peakT,basenum,primary,secondary,consensus,qual,trim" />
+            </actions>
+            <filter>optional_outputs and 'tabular' in optional_outputs</filter>
+        </data>
+    </xml>
+</macros>
diff -r 000000000000 -r 6ec40b104f9d test-data/input1.ab1
Binary file test-data/input1.ab1 has changed
diff -r 000000000000 -r 6ec40b104f9d test-data/input2.scf
Binary file test-data/input2.scf has changed
diff -r 000000000000 -r 6ec40b104f9d test-data/out1.align1.fasta
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/out1.align1.fasta	Tue Oct 12 14:21:06 2021 +0000
@@ -0,0 +1,106 @@
+>Alt1 (Estimated allelic Fraction: 0.52)
+TCNGCNGGNGTNNTCCGTATTTTNNTTGGATCCNCATTCATAGCAAACACCANAAATCCATATTGGGACCACAA
+TCCCAACAAAGACCACTGGACAGAAGCCAACAAGGTAGGAGTGGGAGCATTCGGGCCTGGGTTCACTCCCCCAC
+ACGGAGGCCTTTTGGGGTGGAGCCCTCAGGCTCAAGGCATGCTGACAACATTACCAGCAAATCCGCCTCCTGCC
+TCCACCAATCGACAGTCAGGAAGGCAGCCTACCCCAATCACTCCACCTTTGAGAGACACTCATCCTCAGGCCAT
+GCAGTGGAATTCCACAACATTCCACCAAGCTCTGCAGGATCCCAGAGTAAATCCTGCTGGTGGCTCCAGTTCCG
+GAACAGTGAACCCTGTTCCGACTACTGCCTCACTCATCTCGTCAATCTTCTCGAGGATTGGGGACCCTGCACCG
+AACATGGAAAGCATCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACAGGCGGGGTTTTTCTTGTTGACAAA
+AATCCTCACAATACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCTCCCGTGTGTC
+TTGGCCAAAATTCGCAGTCCCAAACCTCCAGTCACTCACCAACCTCTTGTCCTCCAATTTGTCCTGCCTATCGC
+TGGATGTGTCTGCGGCGGTTTATCATCTTCCTCTTCATCGTGCTGCTATGCCTCATCATCCTGTTGGGTTCGTC
+TGCACTATCAAAGGTATGTTGCCCCGGTTTGTCNTTNNTAATTCCAAGGATCATGAAAGCCACCCACTACGGGA
+ACCNTTGCCGCAAGCTGCACGACTCATTTCTCAAAGGAAANTCNANGNTTTTCCCTCATNGTTGCTGTTCAAAA
+GCTTCGGACGGAAAATGCACATTGTAGTTNCNNATCCCATCATCATGGGNTTTGGGAAATNCCATATGGTANNG
+GGCNNNAGCCCGCNTNNCCT
+>Ref X75657.1 Human hepatitis virus genotype E Bas preS1 preS2 S C X antigens core antigen X protein and polymerase:1-718 forward
+TTCCACAACATTCCACCAAGCTCTGCAGGATCCCAGAGTAAGAGGCCTGTATTTTCCTGCTGGTGGCTCCAGTT
+CCGGAACAGTGAACCCTGTTCCGACTACTGCCTCACTCATCTCGTCAATCTTCTCGAGGATTGGGGACCCTGCA
+CCGAACATGGAAAGCATCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACAGGCGGGGTTTTTCTTGTTGAC
+AAAAATCCTCACAATACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCTCCCGTGT
+GTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAGTCACTCACCAACCTCTTGTCCTCCAATTTGTCCTGGCTAT
+CGCTGGATGTGTCTGCGGCGTTTTATCATCTTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT
+TCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACCACCAGTACGGGACCCTGCC
+GAACCTGCACGACTCTTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTTCAAAACCTTCGGACGGAAAT
+TGCACTTGTATTCCCATCCCATCATCATGGGCTTTCGGAAAATTCCTATGGGAGTGGGCCTCAGCCCGTTTCTC
+CTGGCTCAGTTTACTAGTGCCATTTGTTCAGTGGTTCGCCGGGCTTTCCCCC
+
+Alignment score: 118
+#-------------------------------------------------------------------------
+
+Alt         1 TCNGCNGGNGTNNTCCGTATTTTNNTTGGATCCNCATTCATAGCAAACACCANAAATCCA
+              |                                                           
+Ref         1 T-----------------------------------------------------------
+
+Alt        61 TATTGGGACCACAATCCCAACAAAGACCACTGGACAGAAGCCAACAAGGTAGGAGTGGGA
+                                                                          
+Ref         2 ------------------------------------------------------------
+
+Alt       121 GCATTCGGGCCTGGGTTCACTCCCCCACACGGAGGCCTTTTGGGGTGGAGCCCTCAGGCT
+                                                                          
+Ref         2 ------------------------------------------------------------
+
+Alt       181 CAAGGCATGCTGACAACATTACCAGCAAATCCGCCTCCTGCCTCCACCAATCGACAGTCA
+                                                                          
+Ref         2 ------------------------------------------------------------
+
+Alt       241 GGAAGGCAGCCTACCCCAATCACTCCACCTTTGAGAGACACTCATCCTCAGGCCATGCAG
+                                                                          
+Ref         2 ------------------------------------------------------------
+
+Alt       301 TGGAATTCCACAACATTCCACCAAGCTCTGCAGGATCCCAGAGTAAA------------T
+                    ||||||||||||||||||||||||||||||||||||||||             |
+Ref         2 ------TCCACAACATTCCACCAAGCTCTGCAGGATCCCAGAGTAAGAGGCCTGTATTTT
+
+Alt       349 CCTGCTGGTGGCTCCAGTTCCGGAACAGTGAACCCTGTTCCGACTACTGCCTCACTCATC
+              ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
+Ref        56 CCTGCTGGTGGCTCCAGTTCCGGAACAGTGAACCCTGTTCCGACTACTGCCTCACTCATC
+
+Alt       409 TCGTCAATCTTCTCGAGGATTGGGGACCCTGCACCGAACATGGAAAGCATCACATCAGGA
+              ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
+Ref       116 TCGTCAATCTTCTCGAGGATTGGGGACCCTGCACCGAACATGGAAAGCATCACATCAGGA
+
+Alt       469 TTCCTAGGACCCCTGCTCGTGTTACAGGCGGGGTTTTTCTTGTTGACAAAAATCCTCACA
+              ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
+Ref       176 TTCCTAGGACCCCTGCTCGTGTTACAGGCGGGGTTTTTCTTGTTGACAAAAATCCTCACA
+
+Alt       529 ATACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCTCCCGTG
+              ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
+Ref       236 ATACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCTCCCGTG
+
+Alt       589 TGTCTTGGCCAAAATTCGCAGTCCCAAACCTCCAGTCACTCACCAACCTCTTGTCCTCCA
+              ||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||
+Ref       296 TGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAGTCACTCACCAACCTCTTGTCCTCCA
+
+Alt       649 ATTTGTCCTGCCTATCGCTGGATGTGTCTGCGGCGGTTTATCATCTTCCTCTTCATCGTG
+              |||||||||| |||||||||||||||||||||||| ||||||||||||||||||||| ||
+Ref       356 ATTTGTCCTGGCTATCGCTGGATGTGTCTGCGGCGTTTTATCATCTTCCTCTTCATCCTG
+
+Alt       709 CTGCTATGCCTCATCATCCTGTTGGGTTCGTCTGCACTATCAAAGGTATGTTGCCCCGGT
+              ||||||||||||||| || |||||| ||| |||| |||||||| ||||||||||||  ||
+Ref       416 CTGCTATGCCTCATCTTCTTGTTGG-TTCTTCTGGACTATCAA-GGTATGTTGCCC--GT
+
+Alt       769 TTGTCNTTNNTAATTCCAAGGATCATGAAAGCCACCCACTACGGGAACCNTTGCCGCAAG
+              ||||| |   |||||||| ||||||| ||  ||||| | ||||||| ||  ||||| || 
+Ref       472 TTGTCCTC--TAATTCCA-GGATCATCAA--CCACC-AGTACGGGACCC--TGCCG-AAC
+
+Alt       829 CTGCACGACTCATTTCTCAAAGGAAANTCNANGNTTTTCCCTCATNGTTGCTGTTCAAAA
+              ||||||||||| || ||||| ||||  || | |  |||||||||| ||||||||||||||
+Ref       523 CTGCACGACTC-TTGCTCAA-GGAACCTCTATG--TTTCCCTCAT-GTTGCTGTTCAAAA
+
+Alt       889 GCTTCGGACGGAAAATGCACATTGTAGTTNCNNATCCCATCATCATGGGNTTTGGGAAAT
+               ||||||||||||| ||||| ||||| |  |  |||||||||||||||| ||| ||||| 
+Ref       578 CCTTCGGACGGAAATTGCAC-TTGTATTCCC--ATCCCATCATCATGGGCTTTCGGAAAA
+
+Alt       949 NCCATATGGTANNGGGCNNNAGCCCGCNTNNCCT--------------------------
+                | ||||| |  ||||   ||||||  |  |||                          
+Ref       635 TTCCTATGGGAGTGGGCCTCAGCCCGTTTCTCCTGGCTCAGTTTACTAGTGCCATTTGTT
+
+Alt       983 ------------------------
+                                      
+Ref       695 CAGTGGTTCGCCGGGCTTTCCCCC
+
+#-------------------------------------------------------------------------
+#-------------------------------------------------------------------------
+
+
diff -r 000000000000 -r 6ec40b104f9d test-data/out1.align2.fasta
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/out1.align2.fasta	Tue Oct 12 14:21:06 2021 +0000
@@ -0,0 +1,106 @@
+>Alt2 (Estimated allelic Fraction: 0.28)
+TANTTNTTNGGNNGTCACTCGCCNNGGGGATCANAGTTATGTGGGGACATTANTCATTTTGTTTGGGATTTCCC
+TCTCTTCTTAGAACACTGGACATAAGCCAACAAGGTAGGAGTGGGAGCATTCGGGCCTGGGTTCACTCCCCCAC
+ACGGAGGCCTTTTGGGGTGGAGCACTCAGGCTCAAGGCATGCTGACAACATTACCAGCAAATCCGCCTCCTGCC
+TCCACCAATCGACAGTCAGGAAGGCAGCCTACCCAAATTACACCACCTTTGAGAGACACTCATCCTCAGGCCAT
+GCAGTGGAATTCCACAACATGCCACCAACCTCTGCAGGATCCCAAAGTAAGTCCTGCTGGTGGCTCCAGTTCCG
+GAACAGTGAACCCTGTTCCGACTACAGCCTCACTCATCTCGTCAACCTTCTCGAGGATTGGGGACCCTGCACCG
+AACATGGACAGCATCACATCAGGATTCCGATGACCCCCGCTCATGTTACAAGCGGGGTTTTTCTTGTTGACAAA
+AATAAACAGGATACCGCGTAGACAACAGGCGTGGTGGACTTCTCTCTCTTTTGGAAGGGGAGGACCTTTGTGTG
+ATGGTGGGAAATCTTAGTCCCTCGCCTCCAATCACTCTCCACCCTCTTGTGGGCCTAATTGAGGTGAGTCCCGC
+TGAAAGAAGTGGCAGCGGGGTATCACATACCTCTTCATCTTGCGGGAGAGCCGCCACGTCCTGTGTGGGTGAGC
+TCCCCCAGAACAAAAGGGTGTTGCGGGGGGGGGNGGNNACAAACCGCGGGGGGAAGGAGGGAACCCACTAAAAG
+AACNGGTGCGGTTTCAAAAAAGGGACTGTTTAGTTGAGAANGGNCNGNCATTTTTCATANTTTCTGTGGGTGAG
+TAAAAAACACGGGGGACGCAGATGGGAGGNTNNTCCAAACACCAAAAAANGAGGGAGGGGNAATTTATTCTNNA
+AGGNNNGCGCAAGNCNNTTC
+>Ref X75657.1 Human hepatitis virus genotype E Bas preS1 preS2 S C X antigens core antigen X protein and polymerase:1-714 forward
+TTCCACAACATTCCACCAAGCTCTGCAGGATCCCAGAGTAAGAGGCCTGTATTTTCCTGCTGGTGGCTCCAGTT
+CCGGAACAGTGAACCCTGTTCCGACTACTGCCTCACTCATCTCGTCAATCTTCTCGAGGATTGGGGACCCTGCA
+CCGAACATGGAAAGCATCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACAGGCGGGGTTTTTCTTGTTGAC
+AAAAATCCTCACAATACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCTCCCGTGT
+GTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAGTCACTCACCAACCTCTTGTCCTCCAATTTGTCCTGGCTAT
+CGCTGGATGTGTCTGCGGCGTTTTATCATCTTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT
+TCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACCACCAGTACGGGACCCTGCC
+GAACCTGCACGACTCTTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTTCAAAACCTTCGGACGGAAAT
+TGCACTTGTATTCCCATCCCATCATCATGGGCTTTCGGAAAATTCCTATGGGAGTGGGCCTCAGCCCGTTTCTC
+CTGGCTCAGTTTACTAGTGCCATTTGTTCAGTGGTTCGCCGGGCTTTC
+
+Alignment score: -1120
+#-------------------------------------------------------------------------
+
+Alt         1 TANTTNTTNGGNNGTCACTCGCCNNGGGGATCANAGTTATGTGGGGACATTANTCATTTT
+              |                                                           
+Ref         1 T-----------------------------------------------------------
+
+Alt        61 GTTTGGGATTTCCCTCTCTTCTTAGAACACTGGACATAAGCCAACAAGGTAGGAGTGGGA
+                        |||                                               
+Ref         2 ----------TCC-----------------------------------------------
+
+Alt       121 GCATTCGGGCCTGGGTTCACTCCCCCACACGGAGGCCTTTTGGGGTGGAGCACTCAGGCT
+                                                                          
+Ref         5 ------------------------------------------------------------
+
+Alt       181 CAAGGCATGCTGACAACATTACCAGCAAATCCGCCTCCTGCCTCCACCAATCGACAGTCA
+                          ||||||||                       ||||||| |  | | ||
+Ref         5 ------------ACAACATT-----------------------CCACCAAGCT-CTG-CA
+
+Alt       241 GGAAGGCAGCCTACCCAAATTACACCACCTTTGAGAGACACTCATCCTCAGGCCATGCAG
+              |||          |||| | ||           ||||              |||      
+Ref        28 GGAT---------CCCAGAGTA-----------AGAG--------------GCC------
+
+Alt       301 TGGAATTCCACAACATGCCACCAACCTCTGCAGGATCCCAAAGTAAGTCCTGCTGGTGGC
+              || | ||                                        |||||||||||||
+Ref        48 TGTATTT----------------------------------------TCCTGCTGGTGGC
+
+Alt       361 TCCAGTTCCGGAACAGTGAACCCTGTTCCGACTACAGCCTCACTCATCTCGTCAACCTTC
+              ||||||||||||||||||||||||||||||||||| ||||||||||||||||||| ||||
+Ref        68 TCCAGTTCCGGAACAGTGAACCCTGTTCCGACTACTGCCTCACTCATCTCGTCAATCTTC
+
+Alt       421 TCGAGGATTGGGGACCCTGCACCGAACATGGACAGCATCACATCAGGATTCCGATGACCC
+              |||||||||||||||||||||||||||||||| ||||||||||||||||||| | |||||
+Ref       128 TCGAGGATTGGGGACCCTGCACCGAACATGGAAAGCATCACATCAGGATTCCTAGGACCC
+
+Alt       481 CCGCTCATGTTACAAGCGGGGTTTTTCTTGTTGACAAAAATAAACAGGATACCGCGTAGA
+              | |||| ||||||| ||||||||||||||||||||||||||   ||  |||||||  || 
+Ref       188 CTGCTCGTGTTACAGGCGGGGTTTTTCTTGTTGACAAAAATCCTCACAATACCGCAGAGT
+
+Alt       541 CAACAGGCGTGGTGGACTTCTCTCTCTTTTGGAAGGGGAGGACCTTTGTGTGATGGTGGG
+              | | |  |||||||||||||||||  ||||  | ||||||  ||  |||||  |||    
+Ref       248 CTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCTCCCGTGTGTCTTGGCCAA
+
+Alt       601 AAATCTTAGTCCCTCGCCTCCAATCACTCTCCACCCTCTTGTGGGCCTAATTGAGGTGAG
+              || ||  ||||||   |||||| |||||| ||| ||||||||   || | |||   ||  
+Ref       308 AATTCGCAGTCCCCAACCTCCAGTCACTCACCAACCTCTTGTCCTCCAATTTGTCCTGGC
+
+Alt       661 TCCCGCTGAAAGAAGTGGCAGCGGGGT---ATCACATACCTCTTCATCTTGCGGGAGAGC
+              |  |||||   || ||| | |||| ||   ||||  | |||||||||| ||| |    ||
+Ref       368 TATCGCTG---GATGTGTCTGCGGCGTTTTATCATCTTCCTCTTCATCCTGCTGCTATGC
+
+Alt       718 CGCCACGTCCTGTGTGGGTGAGCTCCCCCAGAACAAAAGGGTGTTGCGGGGGGGGGNGGN
+              | |  | || ||| ||| |   || |   |  |  || |  ||||||  |   |      
+Ref       425 CTCATCTTCTTGT-TGGTT---CTTCTGGACTATCAAGGTATGTTGCCCGTTTGT-----
+
+Alt       778 NACAAACCGCGGGGGGAAGGAGGGAACCCACTAAAAGAACNGGTGCGGTTTCAAAAAAGG
+                    || |       ||||    | | || |  || || ||  |   | |  ||   |
+Ref       476 ------CCTCTAATTCCAGGATC--ATCAACCACCAGTACGGGACCC--TGCCGAACCTG
+
+Alt       838 GACTGTTTAGTTGAGAANGGNCNGNCATTTTTCATANTTTCTGTGGGTGAGTAAAAAACA
+               || |  |  |    || |  |    || ||||     |  ||| | ||  |  ||||| 
+Ref       526 CAC-GACTCTTGCTCAAGGAACCTCTATGTTTCCC---TCATGTTGCTG--TTCAAAAC-
+
+Alt       898 CGGGGGACGCAGATGGGAGGN-TNNTCCAAACACCAAAAAANGAGGGAGGGGNAATTTAT
+              |   ||||| | || | |    |  ||| | | |   |  | | |     || || ||  
+Ref       579 CTTCGGACGGAAATTGCACTTGTATTCCCATCCCATCATCATGGGCTTTCGGAAAATTCC
+
+Alt       957 TCTNNAAGGNNNGCGCAAGNCNNTTC----------------------------------
+              | |   ||     | ||   |  |||                                  
+Ref       639 TATGGGAGTGGGCCTCAGCCCGTTTCTCCTGGCTCAGTTTACTAGTGCCATTTGTTCAGT
+
+Alt       983 ----------------
+                              
+Ref       699 GGTTCGCCGGGCTTTC
+
+#-------------------------------------------------------------------------
+#-------------------------------------------------------------------------
+
+
diff -r 000000000000 -r 6ec40b104f9d test-data/out1.align3.fasta
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/out1.align3.fasta	Tue Oct 12 14:21:06 2021 +0000
@@ -0,0 +1,106 @@
+>Alt1 (Estimated allelic Fraction: 0.52)
+TCNGCNGGNGTNNTCCGTATTTTNNTTGGATCCNCATTCATAGCAAACACCANAAATCCATATTGGGACCACAA
+TCCCAACAAAGACCACTGGACAGAAGCCAACAAGGTAGGAGTGGGAGCATTCGGGCCTGGGTTCACTCCCCCAC
+ACGGAGGCCTTTTGGGGTGGAGCCCTCAGGCTCAAGGCATGCTGACAACATTACCAGCAAATCCGCCTCCTGCC
+TCCACCAATCGACAGTCAGGAAGGCAGCCTACCCCAATCACTCCACCTTTGAGAGACACTCATCCTCAGGCCAT
+GCAGTGGAATTCCACAACATTCCACCAAGCTCTGCAGGATCCCAGAGTAAATCCTGCTGGTGGCTCCAGTTCCG
+GAACAGTGAACCCTGTTCCGACTACTGCCTCACTCATCTCGTCAATCTTCTCGAGGATTGGGGACCCTGCACCG
+AACATGGAAAGCATCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACAGGCGGGGTTTTTCTTGTTGACAAA
+AATCCTCACAATACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCTCCCGTGTGTC
+TTGGCCAAAATTCGCAGTCCCAAACCTCCAGTCACTCACCAACCTCTTGTCCTCCAATTTGTCCTGCCTATCGC
+TGGATGTGTCTGCGGCGGTTTATCATCTTCCTCTTCATCGTGCTGCTATGCCTCATCATCCTGTTGGGTTCGTC
+TGCACTATCAAAGGTATGTTGCCCCGGTTTGTCNTTNNTAATTCCAAGGATCATGAAAGCCACCCACTACGGGA
+ACCNTTGCCGCAAGCTGCACGACTCATTTCTCAAAGGAAANTCNANGNTTTTCCCTCATNGTTGCTGTTCAAAA
+GCTTCGGACGGAAAATGCACATTGTAGTTNCNNATCCCATCATCATGGGNTTTGGGAAATNCCATATGGTANNG
+GGCNNNAGCCCGCNTNNCCT
+>Alt2 (Estimated allelic Fraction: 0.28)
+TANTTNTTNGGNNGTCACTCGCCNNGGGGATCANAGTTATGTGGGGACATTANTCATTTTGTTTGGGATTTCCC
+TCTCTTCTTAGAACACTGGACATAAGCCAACAAGGTAGGAGTGGGAGCATTCGGGCCTGGGTTCACTCCCCCAC
+ACGGAGGCCTTTTGGGGTGGAGCACTCAGGCTCAAGGCATGCTGACAACATTACCAGCAAATCCGCCTCCTGCC
+TCCACCAATCGACAGTCAGGAAGGCAGCCTACCCAAATTACACCACCTTTGAGAGACACTCATCCTCAGGCCAT
+GCAGTGGAATTCCACAACATGCCACCAACCTCTGCAGGATCCCAAAGTAAGTCCTGCTGGTGGCTCCAGTTCCG
+GAACAGTGAACCCTGTTCCGACTACAGCCTCACTCATCTCGTCAACCTTCTCGAGGATTGGGGACCCTGCACCG
+AACATGGACAGCATCACATCAGGATTCCGATGACCCCCGCTCATGTTACAAGCGGGGTTTTTCTTGTTGACAAA
+AATAAACAGGATACCGCGTAGACAACAGGCGTGGTGGACTTCTCTCTCTTTTGGAAGGGGAGGACCTTTGTGTG
+ATGGTGGGAAATCTTAGTCCCTCGCCTCCAATCACTCTCCACCCTCTTGTGGGCCTAATTGAGGTGAGTCCCGC
+TGAAAGAAGTGGCAGCGGGGTATCACATACCTCTTCATCTTGCGGGAGAGCCGCCACGTCCTGTGTGGGTGAGC
+TCCCCCAGAACAAAAGGGTGTTGCGGGGGGGGGNGGNNACAAACCGCGGGGGGAAGGAGGGAACCCACTAAAAG
+AACNGGTGCGGTTTCAAAAAAGGGACTGTTTAGTTGAGAANGGNCNGNCATTTTTCATANTTTCTGTGGGTGAG
+TAAAAAACACGGGGGACGCAGATGGGAGGNTNNTCCAAACACCAAAAAANGAGGGAGGGGNAATTTATTCTNNA
+AGGNNNGCGCAAGNCNNTTC
+
+Alignment score: 730
+#-------------------------------------------------------------------------
+
+Alt1        1 TCNGCNGGNGTNNTCCGTATTTTNNTTGGATCCNCATTCATAGCAAACACCANAAATCCA
+              | |  |  || ||  |       ||  ||||| |  ||    |   |||  ||  ||   
+Alt2        1 TANTTNTTNGGNNGTCACTCGCCNNGGGGATCANAGTTATGTGGGGACATTANTCATTTT
+
+Alt1       61 TATTGGGACCACAATCCCAACAAAGACCACTGGACAGAAGCCAACAAGGTAGGAGTGGGA
+                ||||||   |  || |  |  ||| ||||||||| |||||||||||||||||||||||
+Alt2       61 GTTTGGGATTTCCCTCTCTTCTTAGAACACTGGACATAAGCCAACAAGGTAGGAGTGGGA
+
+Alt1      121 GCATTCGGGCCTGGGTTCACTCCCCCACACGGAGGCCTTTTGGGGTGGAGCCCTCAGGCT
+              ||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||
+Alt2      121 GCATTCGGGCCTGGGTTCACTCCCCCACACGGAGGCCTTTTGGGGTGGAGCACTCAGGCT
+
+Alt1      181 CAAGGCATGCTGACAACATTACCAGCAAATCCGCCTCCTGCCTCCACCAATCGACAGTCA
+              ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
+Alt2      181 CAAGGCATGCTGACAACATTACCAGCAAATCCGCCTCCTGCCTCCACCAATCGACAGTCA
+
+Alt1      241 GGAAGGCAGCCTACCCCAATCACTCCACCTTTGAGAGACACTCATCCTCAGGCCATGCAG
+              |||||||||||||||| ||| || ||||||||||||||||||||||||||||||||||||
+Alt2      241 GGAAGGCAGCCTACCCAAATTACACCACCTTTGAGAGACACTCATCCTCAGGCCATGCAG
+
+Alt1      301 TGGAATTCCACAACATTCCACCAAGCTCTGCAGGATCCCAGAGTAAATCCTGCTGGTGGC
+              |||||||||||||||| ||||||| ||||||||||||||| ||||| |||||||||||||
+Alt2      301 TGGAATTCCACAACATGCCACCAACCTCTGCAGGATCCCAAAGTAAGTCCTGCTGGTGGC
+
+Alt1      361 TCCAGTTCCGGAACAGTGAACCCTGTTCCGACTACTGCCTCACTCATCTCGTCAATCTTC
+              ||||||||||||||||||||||||||||||||||| ||||||||||||||||||| ||||
+Alt2      361 TCCAGTTCCGGAACAGTGAACCCTGTTCCGACTACAGCCTCACTCATCTCGTCAACCTTC
+
+Alt1      421 TCGAGGATTGGGGACCCTGCACCGAACATGGAAAGCATCACATCAGGATTCCTAGGACCC
+              |||||||||||||||||||||||||||||||| ||||||||||||||||||| | |||||
+Alt2      421 TCGAGGATTGGGGACCCTGCACCGAACATGGACAGCATCACATCAGGATTCCGATGACCC
+
+Alt1      481 CTGCTCGTGTTACAGGCGGGGTTTTTCTTGTTGACAAAAATCCTCACAATACCGCAGAGT
+              | |||| ||||||| ||||||||||||||||||||||||||   ||  |||||||  || 
+Alt2      481 CCGCTCATGTTACAAGCGGGGTTTTTCTTGTTGACAAAAATAAACAGGATACCGCGTAGA
+
+Alt1      541 CTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCTCCCGTGTGTCTTGGCCAA
+              | | |  |||||||||||||||||  ||||  | ||||||  ||  |||||  |||    
+Alt2      541 CAACAGGCGTGGTGGACTTCTCTCTCTTTTGGAAGGGGAGGACCTTTGTGTGATGGTGGG
+
+Alt1      601 AATTCGCAGTCCCAAACCTCCAGTCACTCACCAACCTCTTGTCCTCCAATTTGTCCTGCC
+              || ||  ||||||   |||||| |||||| ||| ||||||||   || | |||   ||  
+Alt2      601 AAATCTTAGTCCCTCGCCTCCAATCACTCTCCACCCTCTTGTGGGCCTAATTGAGGTGAG
+
+Alt1      661 TATCGCTGGATGTGTCTGCGGCGGTTTATCATCTTCCTCTTCATCGTGCTGCTATGCCTC
+              |  ||||| | |     || ||||  |||||  | |||||||||| ||| |    ||| |
+Alt2      661 TCCCGCTGAAAGAAGTGGCAGCGGGGTATCACATACCTCTTCATCTTGCGGGAGAGCCGC
+
+Alt1      721 ATCATCCTGT-TGGGTTCG-TCTGCACTATCAAAGGTATGTTGCCCCGGTTTGTCNTTNN
+                | |||||| |||||  | ||  |   | |||| |  ||||||   ||   |  |  ||
+Alt2      721 CACGTCCTGTGTGGGTGAGCTCCCCCAGAACAAAAGGGTGTTGCGGGGGGGGG--NGGNN
+
+Alt1      779 TAATTCCAAGGATC-ATGAAAGCCACCCACTACGGGAACCNTTGCCGC-----AAGCTGC
+                |  ||  ||    | | | |  ||||||||   ||||   ||| |      ||   | 
+Alt2      779 ACAAACCGCGGGGGGAAGGAGGGAACCCACTAAAAGAACNGGTGCGGTTTCAAAAAAGGG
+
+Alt1      833 ACGACTCATTTCTCAAAGGAAANTCNANGNTTTTCCCTCATNGTTGCTGT-------TCA
+              ||   | | ||   || ||     || |  ||||   ||||  || ||||       | |
+Alt2      839 ACTGTTTAGTTGAGAANGGN----CNGNCATTTT---TCATANTTTCTGTGGGTGAGTAA
+
+Alt1      886 AAAGCTTCGGACGGAAAATGCACATTGTAGTTNCNNATCCCATCATCAT-----GGGNTT
+              ||| |     ||||   | ||| || | ||  | || ||| | || ||      | |   
+Alt2      892 AAAAC-----ACGGGGGACGCAGATGGGAGG-NTNN-TCCAAACACCAAAAAANGAGGGA
+
+Alt1      941 TGGGAAATNCCATATGGTANNGGGCNNNAGCCCGCNTNNCCT
+               ||| |||    |||  | |  || ||  ||  | | ||   
+Alt2      945 GGGGNAATT---TATTCTNNAAGGNNNGCGCAAG-NCNNTTC
+
+#-------------------------------------------------------------------------
+#-------------------------------------------------------------------------
+
+
diff -r 000000000000 -r 6ec40b104f9d test-data/out1.alignment.fasta
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/out1.alignment.fasta	Tue Oct 12 14:21:06 2021 +0000
@@ -0,0 +1,4 @@
+>input1 (forward)
+TATTGGGACCACAATCCCAACAAAGACCACTGGACAGAAGCCAACAAGGTAGGAGTGGGAGCATTCGGGCCTGGGTTCACTCCCCCACACGGAGGCCTTTTGGGGTGGAGCCCTCAGGCTCAAGGCATGCTGACAACATTACCAGCAAATCCGCCTCCTGCCTCCACCAATCGACAGTCAGGAAGGCAGCCTACCCCAATCACTCCACCTTTGAGAGACACTCATCCTCAGGCCATGCAGTGGAATTCCACAACATTCCACCAAGCTCTGCAGGATCCCAGAGTAAATCCTGCTGGTGGCTCCAGTTCCGGAACAGTGAACCCTGTTCCGACTACTGCCTCACTCATCTCGTCAATCTTCTCGAGGATTGGGGACCCTGCACCGAACATGGAAAGCATCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACAGGCGGGGTTTTTCTTGTTGACAAAAATCCTCACAATACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCTCCCGTGTGTCTTGGCCAAAATTCTCAGTCCCAAACCTCCAGTCACTCACCAACCTCTTGTCCTCCAATTTGTC
+>input1 (forward)
+TATTGGGACCACAATCCCAACAAAGACCACTGGACAGAAGCCAACAAGGTAGGAGTGGGAGCATTCGGGCCTGGGTTCACTCCCCCACACGGAGGCCTTTTGGGGTGGAGCCCTCAGGCTCAAGGCATGCTGACAACATTACCAGCAAATCCGCCTCCTGCCTCCACCAATCGACAGTCAGGAAGGCAGCCTACCCCAATCACTCCACCTTTGAGAGACACTCATCCTCAGGCCATGCAGTGGAATTCCACAACATTCCACCAAGCTCTGCAGGATCCCAGAGTAAATCCTGCTGGTGGCTCCAGTTCCGGAACAGTGAACCCTGTTCCGACTACTGCCTCACTCATCTCGTCAATCTTCTCGAGGATTGGGGACCCTGCACCGAACATGGAAAGCATCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACAGGCGGGGTTTTTCTTGTTGACAAAAATCCTCACAATACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCTCCCGTGTGTCTTGGCCAAAATTCTCAGTCCCAAACCTCCAGTCACTCACCAACCTCTTGTCCTCCAATTTGTC
diff -r 000000000000 -r 6ec40b104f9d test-data/out1.bcf
Binary file test-data/out1.bcf has changed
diff -r 000000000000 -r 6ec40b104f9d test-data/out1.consensus.fasta
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/out1.consensus.fasta	Tue Oct 12 14:21:06 2021 +0000
@@ -0,0 +1,2 @@
+>Consensus
+TATTGGGACCACAATCCCAACAAAGACCACTGGACAGAAGCCAACAAGGTAGGAGTGGGAGCATTCGGGCCTGGGTTCACTCCCCCACACGGAGGCCTTTTGGGGTGGAGCCCTCAGGCTCAAGGCATGCTGACAACATTACCAGCAAATCCGCCTCCTGCCTCCACCAATCGACAGTCAGGAAGGCAGCCTACCCCAATCACTCCACCTTTGAGAGACACTCATCCTCAGGCCATGCAGTGGAATTCCACAACATTCCACCAAGCTCTGCAGGATCCCAGAGTAAATCCTGCTGGTGGCTCCAGTTCCGGAACAGTGAACCCTGTTCCGACTACTGCCTCACTCATCTCGTCAATCTTCTCGAGGATTGGGGACCCTGCACCGAACATGGAAAGCATCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACAGGCGGGGTTTTTCTTGTTGACAAAAATCCTCACAATACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCTCCCGTGTGTCTTGGCCAAAATTCTCAGTCCCAAACCTCCAGTCACTCACCAACCTCTTGTCCTCCAATTTGTC
diff -r 000000000000 -r 6ec40b104f9d test-data/out1.fasta
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/out1.fasta	Tue Oct 12 14:21:06 2021 +0000
@@ -0,0 +1,4 @@
+>input1
+TTTTTTTTTGAGCGGGTCGAACCGTCACGAAAAGAAAAGGGGAAGAACCATCAGCAGGAGTAATCCGTATTTTAATTGGATCCACATTCATAGCAAACACCAAAAATCCATATTGGGACCACAATCCCAACAAAGACCACTGGACAGAAGCCAACAAGGTAGGAGTGGGAGCATTCGGGCCTGGGTTCACTCCCCCACACGGAGGCCTTTTGGGGTGGAGCCCTCAGGCTCAAGGCATGCTGACAACATTACCAGCAAATCCGCCTCCTGCCTCCACCAATCGACAGTCAGGAAGGCAGCCTACCCCAATCACTCCACCTTTGAGAGACACTCATCCTCAGGCCATGCAGTGGAATTCCACAACATTCCACCAAGCTCTGCAGGATCCCAGAGTAAA------------TCCTGCTGGTGGCTCCAGTTCCGGAACAGTGAACCCTGTTCCGACTACTGCCTCACTCATCTCGTCAATCTTCTCGAGGATTGGGGACCCTGCACCGAACATGGAAAGCATCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACAGGCGGGGTTTTTCTTGTTGACAAAAATCCTCACAATACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCTCCCGTGTGTCTTGGCCAAAATTCTCAGTCCCAAACCTCCAGTCACTCACCAACCTCTTGTCCTCCAATTTGTCCTGCCTATCGCTGGATGTGTCTGCGGCGGTTTATCATATTCCTCTTCATCGTGCTGCTATGCCTCATCATCCTGTTGGGTTCGTCTGCACCATCAAAAGAATGTTGCCCCGGGTTGTGATTAAAAATTCCAAGGAGCAAGAAAGCCACCCACTACGGGAACCAGGGCCGGAAGCTGAACAAGTCATTTTTCAAAGGAAAATGAAAGATTTTCTTTCTTATTTGTGGGGGAAAAGCAAAAAAAGGAAAAAGGAAATTGGGGTTACAAACCCCACCCCCAAGGGATTTGGGAAATACCATATTTTAAAGGGGAAAGGGCCGCATAACCCATTAAAAATTGCATATTTTA-AATTTTTTTTTTTTGAGAAAGAGGGGGGAC--------
+>X75657.1 Human hepatitis virus genotype E Bas preS1 preS2 S C X antigens core antigen X protein and polymerase (forward)
+-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TTCCACAACATTCCACCAAGCTCTGCAGGATCCCAGAGTAAGAGGCCTGTATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTGAACCCTGTTCCGACTACTGCCTCACTCATCTCGTCAATCTTCTCGAGGATTGGGGACCCTGCACCGAACATGGAAAGCATCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACAGGCGGGGTTTTTCTTGTTGACAAAAATCCTCACAATACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCTCCCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAGTCACTCACCAACCTCTTGTCCTCCAATTTGTCCTGGCTATCGCTGGATGTGTCTGCGGCGTTTTATCATCTTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTT-GGTTCTTCTGGACTATCA-AGGTATGTTGCCC--GTTTGTCCT--CTAATTCCA-GGATCATCAA--CCA-CCAGTACGGGACCC--TGCCG-AACCTGCACGACTC-TTGCTCAA-GGAACCTCTATG--TTTCCCTCAT-GTTGCTGTTCAAAACCTTCGGACGGAAATTGCAC-TTGTA--TTCCCATCCCATCATCATGGGCTTTCGGAAAATTCCTATGGGAGTGGGCCTCAGCCCGTTTCTCC---------TGGCTCAGTTTACTAGTGCCATTTGTTCAGTGGTTCGCCGGGCTTTCCCCC
diff -r 000000000000 -r 6ec40b104f9d test-data/out1.txt
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/out1.txt	Tue Oct 12 14:21:06 2021 +0000
@@ -0,0 +1,111 @@
+>Alt
+TTTTTTTTTGAGCGGGTCGAACCGTCACGAAAAGAAAAGGGGAAGAACCATCAGCAGGAGTAATCCGTATTTTA
+ATTGGATCCACATTCATAGCAAACACCAAAAATCCATATTGGGACCACAATCCCAACAAAGACCACTGGACAGA
+AGCCAACAAGGTAGGAGTGGGAGCATTCGGGCCTGGGTTCACTCCCCCACACGGAGGCCTTTTGGGGTGGAGCC
+CTCAGGCTCAAGGCATGCTGACAACATTACCAGCAAATCCGCCTCCTGCCTCCACCAATCGACAGTCAGGAAGG
+CAGCCTACCCCAATCACTCCACCTTTGAGAGACACTCATCCTCAGGCCATGCAGTGGAATTCCACAACATTCCA
+CCAAGCTCTGCAGGATCCCAGAGTAAATCCTGCTGGTGGCTCCAGTTCCGGAACAGTGAACCCTGTTCCGACTA
+CTGCCTCACTCATCTCGTCAATCTTCTCGAGGATTGGGGACCCTGCACCGAACATGGAAAGCATCACATCAGGA
+TTCCTAGGACCCCTGCTCGTGTTACAGGCGGGGTTTTTCTTGTTGACAAAAATCCTCACAATACCGCAGAGTCT
+AGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCTCCCGTGTGTCTTGGCCAAAATTCTCAGTCCCAAA
+CCTCCAGTCACTCACCAACCTCTTGTCCTCCAATTTGTCCTGCCTATCGCTGGATGTGTCTGCGGCGGTTTATC
+ATATTCCTCTTCATCGTGCTGCTATGCCTCATCATCCTGTTGGGTTCGTCTGCACCATCAAAAGAATGTTGCCC
+CGGGTTGTGATTAAAAATTCCAAGGAGCAAGAAAGCCACCCACTACGGGAACCAGGGCCGGAAGCTGAACAAGT
+CATTTTTCAAAGGAAAATGAAAGATTTTCTTTCTTATTTGTGGGGGAAAAGCAAAAAAAGGAAAAAGGAAATTG
+GGGTTACAAACCCCACCCCCAAGGGATTTGGGAAATACCATATTTTAAAGGGGAAAGGGCCGCATAACCCATTA
+AAAATTGCATATTTTAAATTTTTTTTTTTTGAGAAAGAGGGGGGAC
+>Ref X75657.1 Human hepatitis virus genotype E Bas preS1 preS2 S C X antigens core antigen X protein and polymerase:1-718 forward
+TTCCACAACATTCCACCAAGCTCTGCAGGATCCCAGAGTAAGAGGCCTGTATTTTCCTGCTGGTGGCTCCAGTT
+CCGGAACAGTGAACCCTGTTCCGACTACTGCCTCACTCATCTCGTCAATCTTCTCGAGGATTGGGGACCCTGCA
+CCGAACATGGAAAGCATCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACAGGCGGGGTTTTTCTTGTTGAC
+AAAAATCCTCACAATACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCTCCCGTGT
+GTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAGTCACTCACCAACCTCTTGTCCTCCAATTTGTCCTGGCTAT
+CGCTGGATGTGTCTGCGGCGTTTTATCATCTTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT
+TCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACCACCAGTACGGGACCCTGCC
+GAACCTGCACGACTCTTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTTCAAAACCTTCGGACGGAAAT
+TGCACTTGTATTCCCATCCCATCATCATGGGCTTTCGGAAAATTCCTATGGGAGTGGGCCTCAGCCCGTTTCTC
+CTGGCTCAGTTTACTAGTGCCATTTGTTCAGTGGTTCGCCGGGCTTTCCCCC
+
+Alignment score: -906
+#-------------------------------------------------------------------------
+
+Alt         1 TTTTTTTTTGAGCGGGTCGAACCGTCACGAAAAGAAAAGGGGAAGAACCATCAGCAGGAG
+                                                                          
+Ref         1 ------------------------------------------------------------
+
+Alt        61 TAATCCGTATTTTAATTGGATCCACATTCATAGCAAACACCAAAAATCCATATTGGGACC
+                                                                          
+Ref         1 ------------------------------------------------------------
+
+Alt       121 ACAATCCCAACAAAGACCACTGGACAGAAGCCAACAAGGTAGGAGTGGGAGCATTCGGGC
+                                                                          
+Ref         1 ------------------------------------------------------------
+
+Alt       181 CTGGGTTCACTCCCCCACACGGAGGCCTTTTGGGGTGGAGCCCTCAGGCTCAAGGCATGC
+                                                                          
+Ref         1 ------------------------------------------------------------
+
+Alt       241 TGACAACATTACCAGCAAATCCGCCTCCTGCCTCCACCAATCGACAGTCAGGAAGGCAGC
+                                                                          
+Ref         1 ------------------------------------------------------------
+
+Alt       301 CTACCCCAATCACTCCACCTTTGAGAGACACTCATCCTCAGGCCATGCAGTGGAATTCCA
+                                                                     |||||
+Ref         1 -------------------------------------------------------TTCCA
+
+Alt       361 CAACATTCCACCAAGCTCTGCAGGATCCCAGAGTAAA------------TCCTGCTGGTG
+              ||||||||||||||||||||||||||||||||||||             |||||||||||
+Ref         6 CAACATTCCACCAAGCTCTGCAGGATCCCAGAGTAAGAGGCCTGTATTTTCCTGCTGGTG
+
+Alt       409 GCTCCAGTTCCGGAACAGTGAACCCTGTTCCGACTACTGCCTCACTCATCTCGTCAATCT
+              ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
+Ref        66 GCTCCAGTTCCGGAACAGTGAACCCTGTTCCGACTACTGCCTCACTCATCTCGTCAATCT
+
+Alt       469 TCTCGAGGATTGGGGACCCTGCACCGAACATGGAAAGCATCACATCAGGATTCCTAGGAC
+              ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
+Ref       126 TCTCGAGGATTGGGGACCCTGCACCGAACATGGAAAGCATCACATCAGGATTCCTAGGAC
+
+Alt       529 CCCTGCTCGTGTTACAGGCGGGGTTTTTCTTGTTGACAAAAATCCTCACAATACCGCAGA
+              ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
+Ref       186 CCCTGCTCGTGTTACAGGCGGGGTTTTTCTTGTTGACAAAAATCCTCACAATACCGCAGA
+
+Alt       589 GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCTCCCGTGTGTCTTGGCC
+              ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
+Ref       246 GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCTCCCGTGTGTCTTGGCC
+
+Alt       649 AAAATTCTCAGTCCCAAACCTCCAGTCACTCACCAACCTCTTGTCCTCCAATTTGTCCTG
+              ||||||| ||||||| ||||||||||||||||||||||||||||||||||||||||||||
+Ref       306 AAAATTCGCAGTCCCCAACCTCCAGTCACTCACCAACCTCTTGTCCTCCAATTTGTCCTG
+
+Alt       709 CCTATCGCTGGATGTGTCTGCGGCGGTTTATCATATTCCTCTTCATCGTGCTGCTATGCC
+               |||||||||||||||||||||||| |||||||| |||||||||||| ||||||||||||
+Ref       366 GCTATCGCTGGATGTGTCTGCGGCGTTTTATCATCTTCCTCTTCATCCTGCTGCTATGCC
+
+Alt       769 TCATCATCCTGTTGGGTTCGTCTGCACCATCAAAAGAATGTTGCCCCGGGTTGTGATTAA
+              ||||| || |||| ||||| |||| || |||| | | |||||||||  | ||||  |   
+Ref       426 TCATCTTCTTGTT-GGTTCTTCTGGACTATCA-AGGTATGTTGCCC--GTTTGTCCT--C
+
+Alt       829 AAATTCCAAGGAGCAAGAAAGCCACCCACTACGGGAACCAGGGCCGGAAGCTGAACAAGT
+               ||||||| ||| ||  ||  ||| ||| ||||||| ||   |||| || ||| || | |
+Ref       480 TAATTCCA-GGATCATCAA--CCA-CCAGTACGGGACCC--TGCCG-AACCTGCACGACT
+
+Alt       889 CATTTTTCAAAGGAAAATGAAAGATTTTCTTTCTTATTTGTGGGGGAAAAGCAAAAAAAG
+              | ||  |||| ||||  |  | |  ||||  || |  |||  |   |||| |     | |
+Ref       533 C-TTGCTCAA-GGAACCTCTATG--TTTCCCTCAT-GTTGCTGTTCAAAACCTTCGGACG
+
+Alt       949 GAAAAAGGAAATTGGGGTTACAAACCCCACCCCCAAGGGATTTGGGAAATACCATATTTT
+              ||||  | |  |||    | |  | |||| |  || ||| ||| |||||   | |||   
+Ref       588 GAAATTGCAC-TTGTA--TTCCCATCCCATCATCATGGGCTTTCGGAAAATTCCTATGGG
+
+Alt      1009 AAAGGGGAAAGGGCCGCATAACCCATTAAAAATTGCATATTTTA-AATTTTTTTTTTTTG
+              |  |||     | |||  |  ||         | ||  | ||||  | |    ||| || 
+Ref       645 AGTGGGCCTCAGCCCGTTTCTCC---------TGGCTCAGTTTACTAGTGCCATTTGTTC
+
+Alt      1068 AGAAAGAGGGGGGAC--------
+              ||      |  || |        
+Ref       696 AGTGGTTCGCCGGGCTTTCCCCC
+
+#-------------------------------------------------------------------------
+#-------------------------------------------------------------------------
+
+
diff -r 000000000000 -r 6ec40b104f9d test-data/out2.alignment.fasta
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/out2.alignment.fasta	Tue Oct 12 14:21:06 2021 +0000
@@ -0,0 +1,6 @@
+>input1 (forward)
+TATTGGGACCACAATCCCAACAAAGACCACTGGACAGAAGCCAACAAGGTAGGAGTGGGAGCATTCGGGCCTGGGTTCACTCCCCCACACGGAGGCCTTTTGGGGTGGAGCCCTCAGGCTCAAGGCATGCTGACAACATTACCAGCAAATCCGCCTCCTGCCTCCACCAATCGACAGTCAGGAAGGCAGCCTACCCCAATCACTCCACCTTTGAGAGACACTCATCCTCAGGCCATGCAGTGGAATTCCACAACATTCCACCAAGCTCTGCAGGATCCCAGAGTAAA------------TCCTGCTGGTGGCTCCAGTTCCGGAACAGTGAACCCTGTTCCGACTACTGCCTCACTCATCTCGTCAATCTTCTCGAGGATTGGGGACCCTGCACCGAACATGGAAAGCATCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACAGGCGGGGTTTTTCTTGTTGACAAAAATCCTCACAATACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCTCCCGTGTGTCTTGGCCAAAATTCTCAGTCCCAAACCTCCAGTCACTCACCAACCTCTTGTCCTCCAATTTGTC------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
+>input1 (forward)
+TATTGGGACCACAATCCCAACAAAGACCACTGGACAGAAGCCAACAAGGTAGGAGTGGGAGCATTCGGGCCTGGGTTCACTCCCCCACACGGAGGCCTTTTGGGGTGGAGCCCTCAGGCTCAAGGCATGCTGACAACATTACCAGCAAATCCGCCTCCTGCCTCCACCAATCGACAGTCAGGAAGGCAGCCTACCCCAATCACTCCACCTTTGAGAGACACTCATCCTCAGGCCATGCAGTGGAATTCCACAACATTCCACCAAGCTCTGCAGGATCCCAGAGTAAA------------TCCTGCTGGTGGCTCCAGTTCCGGAACAGTGAACCCTGTTCCGACTACTGCCTCACTCATCTCGTCAATCTTCTCGAGGATTGGGGACCCTGCACCGAACATGGAAAGCATCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACAGGCGGGGTTTTTCTTGTTGACAAAAATCCTCACAATACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCTCCCGTGTGTCTTGGCCAAAATTCTCAGTCCCAAACCTCCAGTCACTCACCAACCTCTTGTCCTCCAATTTGTC------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
+>Reference
+-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TTCCACAACATTCCACCAAGCTCTGCAGGATCCCAGAGTAAGAGGCCTGTATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTGAACCCTGTTCCGACTACTGCCTCACTCATCTCGTCAATCTTCTCGAGGATTGGGGACCCTGCACCGAACATGGAAAGCATCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACAGGCGGGGTTTTTCTTGTTGACAAAAATCCTCACAATACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCTCCCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAGTCACTCACCAACCTCTTGTCCTCCAATTTGTCCTGGCTATCGCTGGATGTGTCTGCGGCGTTTTATCATCTTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACCACCAGTACGGGACCCTGCCGAACCTGCACGACTCTTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTTCAAAACCTTCGGACGGAAATTGCACTTGTATTCCCATCCCATCATCATGGGCTTTCGGAAAATTCCTATGGGAGTGGGCCTCAGCCCGTTTCTCCTGGCTCAGTTTACTAGTGCCATTTGTTCAGTGGTTCGCCGGGCTTTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAATCCCAACAAAACAAAAAGATGGGGCTATTCCCTTAATTTCATGGGTTATGTAATTGGAAGTTGGGGCTCATTACCACAGGAACACATCATACAAAAAATCAAAGACTGTTTTAGAAAACTCCCTGTTAACCGGCCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGGGCTTTGCTGCCCCCTTTACACAATGTGGATATCCTGCTTTAATGCCTCTGTATGCATGTACTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTGATGCAACCCCCACTGGCTGGGGCTTGGCCATAGGCATTCAGCGCATGCGCGGAACCTTTGTGGCTCCTCTGCCGATCCATACTGCGGAACTCCTAGCCGCTTGTTTTGCTCGCAGCAGGTCTGGAGCAAAACTTATCGGGACCGATAATTCTGTCGTTCTCTCCCGGAAGTATACATCCTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGCGAGGGACGTCCTTTGTCTACGTCCCGTCAGCGCTGAATCCTGCGGACGACCCGTCTCGGGGTCGCTTGGGGATCTATCGTCCCCTTCTCCGTCTGCCGTTCCAGCCGACCACGGGGCGCACCTCTCTTTACGCGGTCTCCCCGTCTGTTCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGGAGACCACCGTGAACGCCCACCAAATATTGCCCAAGGTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTTAAAGACTGGGAGGAGTCGGGGGAGGAGATTAGATTAAAGGTCTTTGTACTAGGAGGCTGTAGGCATAAATTGGTCTGCGCACCAGCACCATGCAACTTTTTCACCTCTGCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTAAGAGATCTTCTAGATACCGCCTCAGCTCTGTATCGGGATGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCACACTGCACTCAGGCAAGCCATTCTTTGCTGGGGAGAACTAATGACTCTAGCTACCTGGGTGGGTGTAAATTTGGAAGATCCAGCATCCAGGGACCTAGTAGTCAGTTATGTCAATACTAATATGGGCCTAAAGTTCAGGCAATTATTGTGGTTTCACATTTCTTGTCTCACTTTTGGAAGAGAAACCGTCATAGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAGAATACTGTTGTTAGACGAAGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGATCTCAATCGCCGCGTCGCAGAAGATCTCAATCTCCAGCTTCCCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAATTTTACGGGGCTTTACTCTTCTACTATACCTGTCTTTAATCCTAACTGGAAAACTCCATCTTTTCCTGATATTCATTTGCACCAGGACATTATTAACAAATGTGAACAATTTGTAGGTCCTCTAACTGTAAATGAAAAACGAAGATTAAACTTAGTCATGCCTGCTAGATTTTTTCCCATCTCTACGAAATATTTGCCCCTAGAGAAAGGTATAAAACCTTATTATCCAGATAATGTAGTTAATCATTACTTCCAAACCAGACACTATTTACATACCCTATGGAAGGCGGGCATCTTATATAAAAGAGAAACTACACGTAGCGCCTCATTTTGTGGGTCACCTTATTCTTGGGAACAAGAGCTACATCATGGGGCTTTCTTGGACGGTCCCTCTCGAATGGGGGAAGAATATTTCCACCACCAATCCTCTGGGATTTTTTCCCGACCACCAGTTGGATCCAGCATTCAGAGCAAACACCAGAAATCCAGATTGGGACCACAATCCCAACAAAGACCACTGGACAGAAGCCAACAAGGTAGGAGTGGGAGCATTCGGGCCTGGGTTCACTCCCCCACACGGAGGCCTTTTGGGGTGGAGCCCTCAGGCTCAAGGCATGCTAAAAACATTGCCAGCAGATCCGCCTCCTGCCTCCACCAATCGGCAGTCAGGAAGGCAGCCTACCCCAATCACTCCACCTTTGAGAGACACTCATCCTCAGGCCATGCAGTGGAA
diff -r 000000000000 -r 6ec40b104f9d test-data/out2.fasta
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/out2.fasta	Tue Oct 12 14:21:06 2021 +0000
@@ -0,0 +1,4 @@
+>input1
+--------TTTTTTTTTGAGCGGGTCGAACCGTCACGAAAAGAAAAGGGGAAGAACCATCAGCAGGAGTAATCCGTATTTTAATTGGATCCACAT-TCATAGCAAACACCAAAAATCCATATTGGGACCACAATCCCAACAAAGACCACTGGAC-AGAAGCCAACAAGGTAGGAGTGGGAG-CATTCGG-GCCTGGGTTC--ACTCCCCCACACGGAGGCCTTTTGGGGTGGAGCCCTCAGGCTCAAGGCATGCTGACAACATTACCAGCAAATCCGCCTCCTGCCTCCACCAATCGACAGTCAGGAAGGCAGCCTACCCCAATCACTCCACCTTTGAG-AGACACTCATCCTCAGGCCATGCAGTGGAATTCCACAACATTCCACCAAGCTCTGCAGGATCCCAGAGTAAATCCTGCTGGTGGCTCCAGTTCCGGAACAGTGAACCCTG-TTCCGACTACTGCCTCACTCATCTCGTCAATCTTCTCGAGGATTGGGGACCCTGCACCGAACATGGAAAGCATCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACAGGCGGGGTTTTTCTTGTTGACAAAAATCCTCACAATACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCT--CCCGTGTGTCTTGGCCAAAATTCTCAGT--CCCAAACCTCCAGTCACTCACCAACCTCTTGTCCTCCAATTTGTCCTGCCTATCGCTGGATGTGTCTGCGGCGGTTTATCATATTCCT-CTTCATCGTGCTGCT------ATGCCTCATCATCCTGTTGGGTTCGTCTGCACCATCAAAAGAATGTTGCCCCGGGTTGTGATTAAAAATTCCAAGGAGCAAGAAAGCCACCCACTACGGGAACCAGGGCCGGAAGCTGAACAAGTCATTTTTCAAAGGAAAATGAAAGATTTTCTTTCTTATTTGTGGGGGAAAAGCAAAAAAAGGAAAAAGGAAATTGGGGTTACAAACCCCACCCCCAAGGGATTTGGG--AAATACCATATTTTAAAGGGGAAAGGGCCGCATAACCCATTAAAAATTGCATATTTTAAATTTTTTTTTTTTGAGAAAGAGGGGGGAC-------------------
+>X75657.1 (forward)
+AACTAATGACTCTAGCTACCTGGGTGG----GTGTAAATTTGGA-AGATCCAGCATCCAGGGACCTAGTAGTCAGT----TATGTCAATACTAATATGGGCCTAAAGTTCAGGCAATTATTGTGGTTTCAC-ATTTCTTGTCTCACTTTTGGAAGAGAAACCGTCATAG-AGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTCCA-------GCTTAT--------AGACCAC-----CAA---ATGCCCCTATC-TTATCAACACTTCCGGAGAATACTGTTGTTAGACGA-AG--AGGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGATC-TCAATCGCCGC-GTCG-----CAGAAGATCTCAA-----TCTCCAGCTTCCCAATGTTAGTATTCCTTG-GACTCATAAGGTGGGAAATTTTACGGGGCTTTACTCTTCTACTATACCTGTCT--TTAATCCTAACTGGAAAACTCCATCTTTTCCTGATATTCATTTGCACCAGGACATTATT---AACAAATGTGAACAATTTGTA---GGTCCTCTAACTGTAAATGAAAAACG--AAGATTAAACTTAGTC---ATGCCTGCTAGATTTTT-TCCCATCTCTACGAAATATTTGCCCCTAGAGAAAGGTATAAAACCTTATTATCCAGATAATGTAGTTAATCATTACTTCCAAACCAGACACTATTTACATAC--CCTATGGAAG-GCGGGCATCT-TATATAAAAGAGAAACTACACGTAGCGCCTCATTTTGTGGGTCACCTTATTCTTGGGAACAAGAGCTACATCATGGG---GCTTTCTTGGACGGTCCCTC-----TCGAATGGGGGAAGAATATTTCCACCACC--AATCCTCTGGG---------------ATTTTTTCCCG---ACCACCAG--TTGGATCCAGCATTCAG-------AGCAAACACCAGAAA-TCCAGATTGGGACCACAATCCCAACA---AAGACCACTGGACAGAAGCCAACAAGGTAGGAGTGGGAGCATTCGGGCCTGGGTTCACTCCCCCACACGGAGGCCTTTTGGGGTGGAGCCCTCAGGCTCAAGGCATGCTAAAAACATTGCC
diff -r 000000000000 -r 6ec40b104f9d test-data/out2.txt
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/out2.txt	Tue Oct 12 14:21:06 2021 +0000
@@ -0,0 +1,115 @@
+>Alt
+TTTTTTTTTGAGCGGGTCGAACCGTCACGAAAAGAAAAGGGGAAGAACCATCAGCAGGAGTAATCCGTATTTTA
+ATTGGATCCACATTCATAGCAAACACCAAAAATCCATATTGGGACCACAATCCCAACAAAGACCACTGGACAGA
+AGCCAACAAGGTAGGAGTGGGAGCATTCGGGCCTGGGTTCACTCCCCCACACGGAGGCCTTTTGGGGTGGAGCC
+CTCAGGCTCAAGGCATGCTGACAACATTACCAGCAAATCCGCCTCCTGCCTCCACCAATCGACAGTCAGGAAGG
+CAGCCTACCCCAATCACTCCACCTTTGAGAGACACTCATCCTCAGGCCATGCAGTGGAATTCCACAACATTCCA
+CCAAGCTCTGCAGGATCCCAGAGTAAATCCTGCTGGTGGCTCCAGTTCCGGAACAGTGAACCCTGTTCCGACTA
+CTGCCTCACTCATCTCGTCAATCTTCTCGAGGATTGGGGACCCTGCACCGAACATGGAAAGCATCACATCAGGA
+TTCCTAGGACCCCTGCTCGTGTTACAGGCGGGGTTTTTCTTGTTGACAAAAATCCTCACAATACCGCAGAGTCT
+AGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCTCCCGTGTGTCTTGGCCAAAATTCTCAGTCCCAAA
+CCTCCAGTCACTCACCAACCTCTTGTCCTCCAATTTGTCCTGCCTATCGCTGGATGTGTCTGCGGCGGTTTATC
+ATATTCCTCTTCATCGTGCTGCTATGCCTCATCATCCTGTTGGGTTCGTCTGCACCATCAAAAGAATGTTGCCC
+CGGGTTGTGATTAAAAATTCCAAGGAGCAAGAAAGCCACCCACTACGGGAACCAGGGCCGGAAGCTGAACAAGT
+CATTTTTCAAAGGAAAATGAAAGATTTTCTTTCTTATTTGTGGGGGAAAAGCAAAAAAAGGAAAAAGGAAATTG
+GGGTTACAAACCCCACCCCCAAGGGATTTGGGAAATACCATATTTTAAAGGGGAAAGGGCCGCATAACCCATTA
+AAAATTGCATATTTTAAATTTTTTTTTTTTGAGAAAGAGGGGGGAC
+>Ref X75657.1:2091-3110 forward
+AACTAATGACTCTAGCTACCTGGGTGGGTGTAAATTTGGAAGATCCAGCATCCAGGGACCTAGTAGTCAGTTAT
+GTCAATACTAATATGGGCCTAAAGTTCAGGCAATTATTGTGGTTTCACATTTCTTGTCTCACTTTTGGAAGAGA
+AACCGTCATAGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTCCAGCTTATAGACCACCAAATGCCC
+CTATCTTATCAACACTTCCGGAGAATACTGTTGTTAGACGAAGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCG
+CCTCGCAGACGAAGATCTCAATCGCCGCGTCGCAGAAGATCTCAATCTCCAGCTTCCCAATGTTAGTATTCCTT
+GGACTCATAAGGTGGGAAATTTTACGGGGCTTTACTCTTCTACTATACCTGTCTTTAATCCTAACTGGAAAACT
+CCATCTTTTCCTGATATTCATTTGCACCAGGACATTATTAACAAATGTGAACAATTTGTAGGTCCTCTAACTGT
+AAATGAAAAACGAAGATTAAACTTAGTCATGCCTGCTAGATTTTTTCCCATCTCTACGAAATATTTGCCCCTAG
+AGAAAGGTATAAAACCTTATTATCCAGATAATGTAGTTAATCATTACTTCCAAACCAGACACTATTTACATACC
+CTATGGAAGGCGGGCATCTTATATAAAAGAGAAACTACACGTAGCGCCTCATTTTGTGGGTCACCTTATTCTTG
+GGAACAAGAGCTACATCATGGGGCTTTCTTGGACGGTCCCTCTCGAATGGGGGAAGAATATTTCCACCACCAAT
+CCTCTGGGATTTTTTCCCGACCACCAGTTGGATCCAGCATTCAGAGCAAACACCAGAAATCCAGATTGGGACCA
+CAATCCCAACAAAGACCACTGGACAGAAGCCAACAAGGTAGGAGTGGGAGCATTCGGGCCTGGGTTCACTCCCC
+CACACGGAGGCCTTTTGGGGTGGAGCCCTCAGGCTCAAGGCATGCTAAAAACATTGCC
+
+Alignment score: -2113
+#-------------------------------------------------------------------------
+
+Alt         1 --------TTTTTTTTTGAGCGGGTCGAACCGTCACGAAAAGAAAAGGGGAAGAACCATC
+                        | |   |    |||| |    ||    |   | | ||    || | |   
+Ref      2091 AACTAATGACTCTAGCTACCTGGGTGG----GTGTAAATTTGGA-AGATCCAGCATCCAG
+
+Alt        53 AGCAGGAGTAATCCGTATTTTAATTGGATCCACAT-TCATAGCAAACACCAAAAATCCAT
+               |    |||| || ||    ||  |  || |  || |      |||   ||   |   ||
+Ref      2146 GGACCTAGTAGTCAGT----TATGTCAATACTAATATGGGCCTAAAGTTCAGGCAATTAT
+
+Alt       112 ATTGGGACCACAATCCCAACAAAGACCACTGGAC-AGAAGCCAACAAGGTAGGAGTGGGA
+                |||   ||| ||  |       ||   ||||  |||| ||  ||  | || | | || 
+Ref      2202 TGTGGTTTCAC-ATTTCTTGTCTCACTTTTGGAAGAGAAACCGTCATAG-AGTATTTGGT
+
+Alt       171 G-CATTCGG-GCCTGGGTTC--ACTCCCCCACACGGAGGCCTTTTGGGGTGGAGCCCTCA
+              | | || || |  ||| |||  ||||| |||       || | |        || || | 
+Ref      2260 GTCTTTTGGAGTGTGGATTCGCACTCCTCCA-------GCTTAT--------AGACCAC-
+
+Alt       227 GGCTCAAGGCATGCTGACAACATTACCAGCAAATCCGCCTCCTGCCTCCACCAATCGACA
+                  |||   ||||    | | ||| || ||  ||||     | |       |  ||| |
+Ref      2304 ----CAA---ATGCCCCTATC-TTATCAACACTTCCGGAGAATACTGTTGTTAGACGA-A
+
+Alt       287 GTCAGGAAGGCAGCCTACCCCAATCACTCCACCTTTGAG-AGACACTCATCCTCAGGCCA
+              |  ||| |||   ||||    ||  |||||  |     | ||||    ||| |||  |  
+Ref      2355 G--AGGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGATC-TCAATCGC
+
+Alt       346 TGCAGTGGAATTCCACAACATTCCACCAAGCTCTGCAGGATCCCAGAGTAAATCCTGCTG
+               || || |     || || ||  ||      ||| |||  |||||  || | |  | || 
+Ref      2412 CGC-GTCG-----CAGAAGATCTCAA-----TCTCCAGCTTCCCAATGTTAGTATTCCTT
+
+Alt       406 GTGGCTCCAGTTCCGGAACAGTGAACCCTG-TTCCGACTACTGCCTCACTCATCTCGTCA
+              | | |||       || | | |  ||   | ||    || || |   ||   |||  | |
+Ref      2461 G-GACTCATAAGGTGGGAAATTTTACGGGGCTTTACTCTTCTACTATACCTGTCT--TTA
+
+Alt       465 ATCTTCTCGAGGATTGGGGACCCTGCACCGAACATGGAAAGCATCACATCAGGATTCCTA
+              ||| |  |  | |      | | |   | ||   |     ||| ||   ||  |||   |
+Ref      2518 ATCCTAACTGGAAAACTCCATCTTTTCCTGATATTCATTTGCACCAGGACATTATT---A
+
+Alt       525 GGACCCCTGCTCGTGTTACAGGCGGGGTTTTTCTTGTTGACAAAAATCCTCACAATACCG
+                |    ||  |   ||  |   ||   | |   |||  |  |||| |   |  ||    
+Ref      2575 ACAAATGTGAACAATTTGTA---GGTCCTCTAACTGTAAATGAAAAACG--AAGATTAAA
+
+Alt       585 CAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCT--CCCGTGTGTC
+              |  ||||   |  | || | || || | ||   | |||| |      |  ||| |     
+Ref      2630 CTTAGTC---ATGCCTGCTAGATTTTT-TCCCATCTCTACGAAATATTTGCCCCTAGAGA
+
+Alt       643 TTGGCCAAAATTCTCAGT--CCCAAACCTCCAGTCACTCACCAACCTCTTGTCCTCCAAT
+                ||   |||  || | |  ||  |   |  ||| | |||  |    |    |   || |
+Ref      2686 AAGGTATAAAACCTTATTATCCAGATAATGTAGTTAATCATTACTTCCAAACCAGACACT
+
+Alt       701 TTGTCCTGCCTATCGCTGGATGTGTCTGCGGCGGTTTATCATATTCCT-CTTCATCGTGC
+               | | |   |   |  |||| | |   ||  |  | ||| | |      || ||    ||
+Ref      2746 ATTTACATAC--CCTATGGAAG-GCGGGCATCT-TATATAAAAGAGAAACTACACGTAGC
+
+Alt       760 TGCT------ATGCCTCATCATCCTGTTGGGTTCGTCTGCACCATCAAAAGAATGTTGCC
+                ||       ||  ||| | |  | |||||  |    ||  |||||   |   | |  |
+Ref      2802 GCCTCATTTTGTGGGTCACCTTATTCTTGGGAACAAGAGCTACATCATGGG---GCTTTC
+
+Alt       814 CCGGGTTGTGATTAAAAATTCCAAGGAGCAAGAAAGCCACCCACTACGGGAACCAGGGCC
+                ||   ||   |      || || | |  |  ||     |||| ||   || |      
+Ref      2859 TTGGACGGTCCCTC-----TCGAATGGGGGAAGAATATTTCCACCACC--AATCCTCTGG
+
+Alt       874 GGAAGCTGAACAAGTCATTTTTCAAAGGAAAATGAAAGATTTTCTTTCTTATTTGTGGGG
+              |               ||||||    |   |     ||  ||   | |    ||  |   
+Ref      2912 G---------------ATTTTTTCCCG---ACCACCAG--TTGGATCCAGCATTCAG---
+
+Alt       934 GAAAAGCAAAAAAAGGAAAAAGGAAATTGGGGTTACAAACCCCACCCCCAAGGGATTTGG
+                  |||||| |   ||||    | ||||||   |||| ||| ||    |||     |||
+Ref      2949 ----AGCAAACACCAGAAA-TCCAGATTGGGACCACAATCCCAACA---AAGACCACTGG
+
+Alt       994 G--AAATACCATATTTTAAAGGGGAAAGGGCCGCATAACCCATTAAAAATTGCATATTTT
+                  ||  | | |   ||   | |  ||              || |      || |    
+Ref      3001 ACAGAAGCCAACAAGGTAGGAGTGGGAGCATTCGGGCCTGGGTTCACTCCCCCACACGGA
+
+Alt      1052 AAATTTTTTTTTTTTGAGAAAGAGGGGGGAC-------------------
+                  ||||    |         |||    |                    
+Ref      3061 GGCCTTTTGGGGTGGAGCCCTCAGGCTCAAGGCATGCTAAAAACATTGCC
+
+#-------------------------------------------------------------------------
+#-------------------------------------------------------------------------
+
+
diff -r 000000000000 -r 6ec40b104f9d test-data/output1.fasta
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/output1.fasta	Tue Oct 12 14:21:06 2021 +0000
@@ -0,0 +1,2 @@
+>primary
+TTTTTTTTTGAGCGGGTCGNACCGTCACGAANAGNAAAGGGGAAGAACCATCNGCNGGNGTNNTCCGTATTTTNNTTGGATCCNCATTCATAGCAAACACCANAAATCCATATTGGGACCACAATCCCAACAAAGACCACTGGACAGAAGCCAACAAGGTAGGAGTGGGAGCATTCGGGCCTGGGTTCACTCCCCCACACGGAGGCCTTTTGGGGTGGAGCCCTCAGGCTCAAGGCATGCTGACAACATTACCAGCAAATCCGCCTCCTGCCTCCACCAATCGACAGTCAGGAAGGCAGCCTACCCCAATCACTCCACCTTTGAGAGACACTCATCCTCAGGCCATGCAGTGGAATTCCACAACATTCCACCAAGCTCTGCAGGATCCCAGAGTAAATCCTGCTGGTGGCTCCAGTTCCGGAACAGTGAACCCTGTTCCGACTACTGCCTCACTCATCTCGTCAATCTTCTCGAGGATTGGGGACCCTGCACCGAACATGGAAAGCATCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACAGGCGGGGTTTTTCTTGTTGACAAAAATCCTCACAATACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCTCCCGTGTGTCTTGGCCAAAATTCTCAGTCCCAAACCTCCAGTCACTCACCAACCTCTTGTCCTCCAATTTGTCCTGCCTATCGCTGGATGTGTCTGCGGCGGTTTATCATATTCCTCTTCATCGTGCTGCTATGCCTCATCATCCTGTTGGGTTCGTCTGCACCATCAAAAGAATGTTGCCCCGGGTTGTGNTTNNAAATTCCAAGGAGCAAGAAAGCCACCCACTACGGGAACCNTGGCCGCAAGCTGCACAAGTCATTTTTCAAAGGAAANTGNANGNTTTTCTTTCTTNTTTGTGGGGGAAAAGCAAAAAAAGGAAAAAGGAAATTGGGGTTNCNNACCCCACCCCCAAGGGNTTTGGGAAATNCCATATTTTANNGGGGNNNGGGCCGCNTNNCCCNTTNNNNNTTGCNTATTTTAAATTTTTTTTTTTTGNGAAAGAGGGGGGAC
diff -r 000000000000 -r 6ec40b104f9d test-data/output1.json
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/output1.json	Tue Oct 12 14:21:06 2021 +0000
@@ -0,0 +1,12 @@
+{
+"pos": [1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 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"9839":"793:C", "9856":"794:A|C", "9867":"795:C", "9879":"796:C|G|T", "9893":"797:A", "9906":"798:T|G", "9919":"799:C|A", "9928":"800:A", "9938":"801:A|C", "9949":"802:A", "9957":"803:A|G", "9970":"804:G|A", "9988":"805:A|T", "9998":"806:A|G", "10008":"807:T|G", "10026":"808:G", "10039":"809:T", "10048":"810:T|G", "10065":"811:G|T", "10077":"812:C|T", "10086":"813:C|G", "10093":"814:C", "10102":"815:C|G", "10117":"816:G", "10124":"817:G", "10140":"818:G|T", "10150":"819:T|G", "10157":"820:T|G", "10175":"821:G", "10191":"822:T|G", "10201":"823:G|C|T", "10207":"824:N", "10218":"825:T|A|G", "10229":"826:T|A|G", "10240":"827:N", "10253":"828:N", "10267":"829:A|C|T", "10276":"830:A|C", "10290":"831:A", "10297":"832:T|A", "10305":"833:T|A", "10318":"834:C", "10327":"835:C", "10339":"836:A|G", "10348":"837:A|C", "10362":"838:G", "10371":"839:G", "10388":"840:A|G", "10398":"841:G|T", "10414":"842:C|A|G", "10424":"843:A|G", "10431":"844:A|G|T", "10442":"845:G|A", "10450":"846:A|C|G", "10466":"847:A|G", "10476":"848:A", "10487":"849:G", "10500":"850:C|G", "10506":"851:C|G", "10521":"852:A", "10528":"853:C|A", "10539":"854:C", "10550":"855:C", "10565":"856:A|C", "10572":"857:C|A|T", "10590":"858:T|A|C", "10604":"859:A|T", "10615":"860:C|A", "10626":"861:G|A", "10636":"862:G|A", "10647":"863:G|A", "10667":"864:A|G", "10676":"865:A", "10684":"866:C|A|G", "10694":"867:C", "10714":"868:N", "10726":"869:T|A|G", "10732":"870:G|T", "10737":"871:G|T", "10757":"872:C|G|T", "10765":"873:C", "10785":"874:G", "10790":"875:C|G|T", "10802":"876:A|T", "10812":"877:A|C|T", "10826":"878:G|A|T", "10835":"879:C", "10854":"880:T|A|G", "10870":"881:G|A", "10884":"882:C|A|T", "10887":"883:A", "10908":"884:C|A", "10923":"885:A|G", "10937":"886:A|G", "10947":"887:G|C|T", "10966":"888:T|A|G", "10980":"889:C|A|G", "10993":"890:A|C", "11001":"891:T", "11013":"892:T|G", "11029":"893:T", "11039":"894:T|C", "11047":"895:T", "11064":"896:C|A", "11069":"897:A|G", "11078":"898:A|G|T", "11093":"899:A|T", "11108":"900:G", "11119":"901:G|A", "11133":"902:A|G", "11144":"903:A", "11157":"904:A", "11165":"905:N", "11183":"906:T|A|G", "11201":"907:G|A|C", "11208":"908:N", "11229":"909:A|C", "11242":"910:N", "11254":"911:G", "11257":"912:N", "11270":"913:T|A|C", "11282":"914:T|A|C", "11290":"915:T", "11296":"916:T", "11318":"917:C|A|T", "11333":"918:T|A|C", "11343":"919:T|C", "11353":"920:T|C", "11367":"921:C|A", "11390":"922:T|A", "11399":"923:T|A|C", "11411":"924:N", "11423":"925:T|C|G", "11432":"926:T", "11440":"927:T", "11459":"928:G|C", "11468":"929:T|C", "11491":"930:G|T", "11500":"931:G|T", "11507":"932:G|T", "11516":"933:G|T", "11547":"934:G|C|T", "11567":"935:A|G|T", "11577":"936:A|G|T", "11595":"937:A", "11609":"938:A|G", "11614":"939:G|T", "11622":"940:C|A", "11644":"941:A|C|T", "11651":"942:A|C|T", "11668":"943:A|C|T", "11681":"944:A|C|G", "11705":"945:A|C|G", "11715":"946:A|C", "11725":"947:A|C", "11739":"948:G|C", "11754":"949:G", "11777":"950:A|C|G", "11786":"951:A|G", "11804":"952:A|G", "11812":"953:A|G", "11827":"954:A|G|T", "11849":"955:G|A|C", "11858":"956:G|A|C", "11873":"957:A|C|G", "11883":"958:A|C", "11897":"959:A|C|G", "11907":"960:T|A|G", "11923":"961:T", "11931":"962:G", "11957":"963:G|T", "11967":"964:G|A|T", "11978":"965:G|A", "11987":"966:T|G", "11998":"967:T|C|G", "12021":"968:N", "12030":"969:C|G|T", "12043":"970:N", "12053":"971:N", "12066":"972:A|G|T", "12077":"973:C|A|T", "12090":"974:C", "12106":"975:C|A", "12114":"976:C|A", "12133":"977:A", "12137":"978:C|A|T", "12155":"979:C|A|T", "12164":"980:C|A|T", "12193":"981:C|A|T", "12202":"982:C|A", "12222":"983:A", "12229":"984:A|C|T", "12247":"985:G|A", "12263":"986:G|A", "12278":"987:G|A", "12298":"988:N", "12325":"989:T|A|G", "12334":"990:T|A", "12343":"991:T|G", "12362":"992:G", "12380":"993:G", "12400":"994:G|A", "12420":"995:A|G|T", "12436":"996:A|G|T", "12451":"997:A|G|T", "12464":"998:T|G", "12490":"999:N", "12498":"1000:C|A", "12507":"1001:C|A|G", "12531":"1002:A|T", "12534":"1003:T", "12547":"1004:A|T", "12565":"1005:T|A|G", "12582":"1006:T|A|G", "12595":"1007:T|G", "12604":"1008:T|A|C", "12622":"1009:A|C|T", "12636":"1010:N", "12645":"1011:N", "12663":"1012:G|A|T", "12678":"1013:G|A|C", "12707":"1014:G", "12722":"1015:G|A|C", "12740":"1016:N", "12756":"1017:N", "12768":"1018:N", "12786":"1019:G|A|C", "12803":"1020:G|C", "12817":"1021:G|A|C", "12823":"1022:C", "12844":"1023:C|A|G", "12848":"1024:G|A", "12856":"1025:C|A|G", "12873":"1026:N", "12890":"1027:T|A|C", "12914":"1028:N", "12924":"1029:N", "12944":"1030:C|T", "12962":"1031:C|T", "12972":"1032:C|G|T", "12992":"1033:N", "13009":"1034:T|C|G", "13024":"1035:T|A|G", "13042":"1036:N", "13053":"1037:N", "13084":"1038:N", "13096":"1039:N", "13109":"1040:N", "13140":"1041:T|C|G", "13152":"1042:T|C|G", "13167":"1043:G|A|T", "13176":"1044:C|A", "13197":"1045:N", "13218":"1046:T|A|C", "13237":"1047:A|T", "13249":"1048:T|G", "13267":"1049:T|A|G", "13277":"1050:T|A|G", "13301":"1051:T|A", "13311":"1052:A|T", "13323":"1053:A|C|T", "13345":"1054:A|G", "13362":"1055:T|A", "13372":"1056:T|A|G", "13389":"1057:T|G", "13417":"1058:T|G", "13428":"1059:T|G", "13438":"1060:T|C|G", "13468":"1061:T|C|G", "13478":"1062:T|G", "13512":"1063:T|G", "13525":"1064:T|C|G", "13535":"1065:T|C|G", "13552":"1066:T|C|G", "13566":"1067:G|A|C", "13581":"1068:N", "13594":"1069:G|A|T", "13624":"1070:A|G", "13641":"1071:A|G", "13657":"1072:A|G|T", "13670":"1073:G|A|T", "13694":"1074:A|T", "13701":"1075:G", "13711":"1076:G|T", "13732":"1077:G|C|T", "13765":"1078:G|C", "13778":"1079:G|C", "13790":"1080:G|A|C", "13820":"1081:A|C|G", "13826":"1082:C|A"},
+"primarySeq": "TTTTTTTTTGAGCGGGTCGNACCGTCACGAANAGNAAAGGGGAAGAACCATCNGCNGGNGTNNTCCGTATTTTNNTTGGATCCNCATTCATAGCAAACACCANAAATCCATATTGGGACCACAATCCCAACAAAGACCACTGGACAGAAGCCAACAAGGTAGGAGTGGGAGCATTCGGGCCTGGGTTCACTCCCCCACACGGAGGCCTTTTGGGGTGGAGCCCTCAGGCTCAAGGCATGCTGACAACATTACCAGCAAATCCGCCTCCTGCCTCCACCAATCGACAGTCAGGAAGGCAGCCTACCCCAATCACTCCACCTTTGAGAGACACTCATCCTCAGGCCATGCAGTGGAATTCCACAACATTCCACCAAGCTCTGCAGGATCCCAGAGTAAATCCTGCTGGTGGCTCCAGTTCCGGAACAGTGAACCCTGTTCCGACTACTGCCTCACTCATCTCGTCAATCTTCTCGAGGATTGGGGACCCTGCACCGAACATGGAAAGCATCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACAGGCGGGGTTTTTCTTGTTGACAAAAATCCTCACAATACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCTCCCGTGTGTCTTGGCCAAAATTCTCAGTCCCAAACCTCCAGTCACTCACCAACCTCTTGTCCTCCAATTTGTCCTGCCTATCGCTGGATGTGTCTGCGGCGGTTTATCATATTCCTCTTCATCGTGCTGCTATGCCTCATCATCCTGTTGGGTTCGTCTGCACCATCAAAAGAATGTTGCCCCGGGTTGTGNTTNNAAATTCCAAGGAGCAAGAAAGCCACCCACTACGGGAACCNTGGCCGCAAGCTGCACAAGTCATTTTTCAAAGGAAANTGNANGNTTTTCTTTCTTNTTTGTGGGGGAAAAGCAAAAAAAGGAAAAAGGAAATTGGGGTTNCNNACCCCACCCCCAAGGGNTTTGGGAAATNCCATATTTTANNGGGGNNNGGGCCGCNTNNCCCNTTNNNNNTTGCNTATTTTAAATTTTTTTTTTTTGNGAAAGAGGGGGGAC",
+"secondarySeq": "MGTTTTTTTCATAWGGTTGNTWAGAGTAGTSNSMNACAMMTASCYSGKTCTANYWNTTNGGNNRTCWCYSRCCNNGSGGATCANAGTTATGTGRGGACATTANTYATTTKGTTTGGGATTTCYCTCTCTTCTTAGAACACTGGACATAAGCCAACAAGGTAGGAGTGGGAGCATTCGGGCCTGGGTTCACTCCCCCACACGGAGGCCTTTTGGGGTGGAGCACTCAGGCTCAAGGCATGCTGACAACATTACCAGCAAATCCGCCTCCTGCCTCCACCAATCGACAGTCAGGAAGGCAGCCTACCCAAATTACACCACCTTTGAGAGACACTCATCCTCAGGCCATGCAGTGGAATTCCACAACATGCCACCAACCTCTGCAGGATCCCAAAGTAAGTCCTGCTGGTGGCTCCAGTTCCGGAACAGTGAACCCTGTTCCGACTACAGCCTCACTCATCTCGTCAACCTTCTCGAGGATTGGGGACCCTGCACCGAACATGGACAGCATCACATCAGGATTCCGATGACCCCCGCTCATGTTACAAGCGGGGTTTTTCTTGTTGACAAAAATAAACAGGATACCGCGYAGACRACAGGCGTGGTGGACTTCTCTCTCTTTTGGAAGGGGAGRACCTTTGTGTGRTGGTGGGAAATCGTAGTCCCTCGCCTCCAMTCACTCKCCACCCTCTTGTGGGCCTAATTGAGGTGAGTSCCGCTGAAAGAAGKGGCWGCGGGGTATCACCTACCTCTTCATCWTGCGGGRGAGCCGCCACGTCCTGTGTGGGTRAGCTCCCCKAGAACAGATGGGTGTTGCGGGTGGGGYNRRNNYCAAACCGCGGGTRGKASGAGGGAACCCWMTAAAAGARCNRTTKCGKTYWCRAWAAGGYRRCTGTCTAGKTGAGAANRMNCNGNMMTTWMCCAAMNSTTCCTTTTYKKAGTAYYYSSCCCGSGGGKMMSCSRTGTWAGSNKNNKWCAAAWWWWAAYAAANRAGGGAKKKGNARTTTRRGMYNNWMGMNNNMCMCRARNMNNTTKNSRNNNNNSSWANMTGRRATYGARGGGSSGGSSSMNWGGKWTGTYCCMSA"
+
+}
diff -r 000000000000 -r 6ec40b104f9d test-data/output2.fasta
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/output2.fasta	Tue Oct 12 14:21:06 2021 +0000
@@ -0,0 +1,2 @@
+>primary
+TTTTTTTTTGAGCGGGTCGNACCGTCACGAANAGNANANGGCAAGAACCATCNGCNGGNGTNNTCCGTANTTTNNNTGGATCCNNATTCATAGCAAACACCANAAATCCATATTGGGACCACAATCCCAACAAAGACCACTGGACAGAAGCCAACAAGGTAGGAGTGGGAGCATTCGGGCCTGGGTTCACTCCCCCACACGGAGGCCTTTTGGGGTGGAGCCCTCAGGCTCAAGGCATGCTGACAACATTACCAGCAAATCCGCCTCCTGCCTCCACCAATCGACAGTCAGGAAGGCAGCCTACCCCAATCACTCCACCTTTGAGAGACACTCATCCTCAGGCCATGCAGTGGAATTCCACAACATTCCACCAAGCTCTGCAGGATCCCAGAGTAAATCCTGCTGGTGGCTCCAGTTCCGGAACAGTGAACCCTGTTCCGACTACTGCCTCACTCATCTCGTCAATCTTCTCGAGGATTGGGGACCCTGCACCGAACATGGAAAGCATCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACAGGCGGGGTTTTTCTTGTTGACAAAAATCCTCACAATACCGCAGAGTCTAGACTCGTGATGGACTTCTCTCAATTTTCTAGGGGGAGCTCCCGTGTGTCTTGGCCAAAATTCTCAGTCGCAAACCTCCAGTCACTCACCAACCTCTTGTCCTCCNATTTGTCCTGCCTATCGCTGGATGTGTNTGCGGCGGTTTATCATATTCATCTTCATCGTGCTGCTATGCCTCATCATCCTGTTGGGTTCGTNTGCACCATCAAAAGAATGTTGCCCCGGGTTGTGNTTNNNAATTCCAAAGAGCAAGAAAGCCACCCANTACGNGAANCNTGGCCGCANGCTGCACAAGTCATNTTTCAAAGGAAANTGNANGNTTATNNTTCTNNTTTGTGGGGGNNAAGCAAAAAAAGGAAAANGGANANTGGNGTNNCNNACCCCACNCCCAANGGNTTTGGGAAATNCNATATTNNNNNGGGGNNNNGGCCGCNTNNCCCNNTNNNNNNNGCNTNTTTNANATNNTTTTTTNTTGNNAAAGAGGGGNGAC
diff -r 000000000000 -r 6ec40b104f9d test-data/output3.fasta
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/output3.fasta	Tue Oct 12 14:21:06 2021 +0000
@@ -0,0 +1,2 @@
+>primary
+GAAATTCTCTTGTTCTACTTCAGCAGTAGTTGTAAGGCTTGCATGAAAGTTATTTGTTCGAAACTATTCTCTGTTACTTTCAATCTGTGCCGTACTTCTTCAATCAACCACCTTATTTCCTCAAATTTCTGTCCCAATTGCTCTCGCCACTTTTCATTCCTGTTCTGGAGGTAGTGAAGATCACCCATCCTCATTACGGTTTCTCCAAGCGAATCTCTGTAGATTTTCAGAGCCTCGAACCGTGTTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTTGACATCCTCATTAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACGATTGCTCCCTCTTCAGTGAAAGCCCTTAGTAGTATCAAAGTCTCCAATCGACTAAAGACCACACTGAAGGG
diff -r 000000000000 -r 6ec40b104f9d test-data/reference1.fasta
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/reference1.fasta	Tue Oct 12 14:21:06 2021 +0000
@@ -0,0 +1,48 @@
+>X75657.1 Human hepatitis virus (genotype E, Bas) preS1, preS2, S, C, X, antigens, core antigen, X protein and polymerase
+TTCCACAACATTCCACCAAGCTCTGCAGGATCCCAGAGTAAGAGGCCTGTATTTTCCTGCTGGTGGCTCC
+AGTTCCGGAACAGTGAACCCTGTTCCGACTACTGCCTCACTCATCTCGTCAATCTTCTCGAGGATTGGGG
+ACCCTGCACCGAACATGGAAAGCATCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACAGGCGGGGTT
+TTTCTTGTTGACAAAAATCCTCACAATACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTA
+GGGGGAGCTCCCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAGTCACTCACCAACCTCTTGTC
+CTCCAATTTGTCCTGGCTATCGCTGGATGTGTCTGCGGCGTTTTATCATCTTCCTCTTCATCCTGCTGCT
+ATGCCTCATCTTCTTGTTGGTTCTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGA
+TCATCAACCACCAGTACGGGACCCTGCCGAACCTGCACGACTCTTGCTCAAGGAACCTCTATGTTTCCCT
+CATGTTGCTGTTCAAAACCTTCGGACGGAAATTGCACTTGTATTCCCATCCCATCATCATGGGCTTTCGG
+AAAATTCCTATGGGAGTGGGCCTCAGCCCGTTTCTCCTGGCTCAGTTTACTAGTGCCATTTGTTCAGTGG
+TTCGCCGGGCTTTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGT
+ACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAATCCC
+AACAAAACAAAAAGATGGGGCTATTCCCTTAATTTCATGGGTTATGTAATTGGAAGTTGGGGCTCATTAC
+CACAGGAACACATCATACAAAAAATCAAAGACTGTTTTAGAAAACTCCCTGTTAACCGGCCTATTGATTG
+GAAAGTATGTCAAAGAATTGTGGGTCTTTTGGGCTTTGCTGCCCCCTTTACACAATGTGGATATCCTGCT
+TTAATGCCTCTGTATGCATGTACTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTC
+TGTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTGA
+TGCAACCCCCACTGGCTGGGGCTTGGCCATAGGCATTCAGCGCATGCGCGGAACCTTTGTGGCTCCTCTG
+CCGATCCATACTGCGGAACTCCTAGCCGCTTGTTTTGCTCGCAGCAGGTCTGGAGCAAAACTTATCGGGA
+CCGATAATTCTGTCGTTCTCTCCCGGAAGTATACATCCTTTCCATGGCTGCTAGGCTGTGCTGCCAACTG
+GATCCTGCGAGGGACGTCCTTTGTCTACGTCCCGTCAGCGCTGAATCCTGCGGACGACCCGTCTCGGGGT
+CGCTTGGGGATCTATCGTCCCCTTCTCCGTCTGCCGTTCCAGCCGACCACGGGGCGCACCTCTCTTTACG
+CGGTCTCCCCGTCTGTTCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATG
+GAGACCACCGTGAACGCCCACCAAATATTGCCCAAGGTCTTACATAAGAGGACTCTTGGACTCTCTGCAA
+TGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTTAAAGACTGGGAGGAGTCGGGGGAGGA
+GATTAGATTAAAGGTCTTTGTACTAGGAGGCTGTAGGCATAAATTGGTCTGCGCACCAGCACCATGCAAC
+TTTTTCACCTCTGCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTG
+GCTTTGGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTTTTGCCT
+TCTGACTTCTTTCCTTCAGTAAGAGATCTTCTAGATACCGCCTCAGCTCTGTATCGGGATGCCTTAGAGT
+CTCCTGAGCATTGTTCACCTCACCACACTGCACTCAGGCAAGCCATTCTTTGCTGGGGAGAACTAATGAC
+TCTAGCTACCTGGGTGGGTGTAAATTTGGAAGATCCAGCATCCAGGGACCTAGTAGTCAGTTATGTCAAT
+ACTAATATGGGCCTAAAGTTCAGGCAATTATTGTGGTTTCACATTTCTTGTCTCACTTTTGGAAGAGAAA
+CCGTCATAGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTCCAGCTTATAGACCACCAAATGC
+CCCTATCTTATCAACACTTCCGGAGAATACTGTTGTTAGACGAAGAGGCAGGTCCCCTAGAAGAAGAACT
+CCCTCGCCTCGCAGACGAAGATCTCAATCGCCGCGTCGCAGAAGATCTCAATCTCCAGCTTCCCAATGTT
+AGTATTCCTTGGACTCATAAGGTGGGAAATTTTACGGGGCTTTACTCTTCTACTATACCTGTCTTTAATC
+CTAACTGGAAAACTCCATCTTTTCCTGATATTCATTTGCACCAGGACATTATTAACAAATGTGAACAATT
+TGTAGGTCCTCTAACTGTAAATGAAAAACGAAGATTAAACTTAGTCATGCCTGCTAGATTTTTTCCCATC
+TCTACGAAATATTTGCCCCTAGAGAAAGGTATAAAACCTTATTATCCAGATAATGTAGTTAATCATTACT
+TCCAAACCAGACACTATTTACATACCCTATGGAAGGCGGGCATCTTATATAAAAGAGAAACTACACGTAG
+CGCCTCATTTTGTGGGTCACCTTATTCTTGGGAACAAGAGCTACATCATGGGGCTTTCTTGGACGGTCCC
+TCTCGAATGGGGGAAGAATATTTCCACCACCAATCCTCTGGGATTTTTTCCCGACCACCAGTTGGATCCA
+GCATTCAGAGCAAACACCAGAAATCCAGATTGGGACCACAATCCCAACAAAGACCACTGGACAGAAGCCA
+ACAAGGTAGGAGTGGGAGCATTCGGGCCTGGGTTCACTCCCCCACACGGAGGCCTTTTGGGGTGGAGCCC
+TCAGGCTCAAGGCATGCTAAAAACATTGCCAGCAGATCCGCCTCCTGCCTCCACCAATCGGCAGTCAGGA
+AGGCAGCCTACCCCAATCACTCCACCTTTGAGAGACACTCATCCTCAGGCCATGCAGTGGAA
+
diff -r 000000000000 -r 6ec40b104f9d tracy_decompose.xml
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/tracy_decompose.xml	Tue Oct 12 14:21:06 2021 +0000
@@ -0,0 +1,124 @@
+<tool id="tracy_decompose" name="tracy Decompose" version="@TOOL_VERSION@+galaxy0" profile="20.09">
+    <description>heterozygous mutations (and call variants)</description>
+    <macros>
+        <import>macros.xml</import>
+    </macros>    
+    <expand macro="requirements" /> 
+    <expand macro="version_command" />
+    <command detect_errors="exit_code"><![CDATA[
+        #if $index_genome == True
+            bgzip -c '$genome' > genome.fasta.gz ;
+            tracy index -o genome.fasta.fm9 genome.fasta.gz &&
+            #set refgenome = "genome.fasta.gz"
+        #else
+            #set refgenome = $genome
+        #end if
+        tracy decompose 
+          --genome '$refgenome'
+          $callVariants
+          --pratio $pratio 
+          --kmer $kmer.kmer 
+          --support $kmer.support 
+          --maxindel $maxindel 
+          --trim $trim.trim 
+          --trimLeft $trim.trimLeft 
+          --trimRight $trim.trimRight 
+          --linelimit $linelimit 
+          --gapopen $alignment.gapopen
+          --gapext $alignment.gapext
+          --match $alignment.match
+          --mismatch $alignment.mismatch
+          '$tracefile'
+    ]]></command>
+    <inputs>
+        <param argument="--genome" type="data" format="fasta,ab1,scf" label="FASTA, ABI or SCF format genome" />
+        <param name="tracefile" type="data" format="ab1,scf" label="Sanger chromatogram tracefile to align" />
+        <param name="index_genome" type="boolean" label="Pre-index the reference" help="Pre-indexing builds a FM index of the reference. This is required for sequences larger than 50 kilobases" />
+        <param argument="--callVariants" type="boolean" truevalue="--callVariants" falsevalue="" label="Call variants in chromatogram" />
+        <!-- annotate option is not yet supported -->
+        <expand macro="common_options" />
+        <expand macro="kmer_options" />
+        <expand macro="alignment_options" />
+        <expand macro="trim_options" />
+        <expand macro="optional_outputs" />
+    </inputs>
+    <outputs>
+        <expand macro="json_output" toolname="decompose" />
+        <data name="out_fasta1" format="fasta" from_work_dir="out.align1" label="tracy decompose allele1 on ${on_string}" />
+        <data name="out_fasta2" format="fasta" from_work_dir="out.align2" label="tracy decompose allele2 on ${on_string}" />
+        <data name="out_fasta3" format="fasta" from_work_dir="out.align3" label="tracy decompose both alleles on ${on_string}" />
+        <expand macro="tabular_output" toolname="decompose" />
+        <data name="out_bcf" format="bcf" from_work_dir="out.bcf" label="tracy decompose variants on ${on_string}">
+            <filter>callVariants</filter>
+        </data>
+    </outputs>
+    <tests>
+        <test expect_num_outputs="3">
+            <param name="genome" value="reference1.fasta" ftype="fasta" />
+            <param name="tracefile" value="input1.ab1" ftype="ab1" />
+            <output name="out_fasta1" value="out1.align1.fasta" ftype="fasta" />    
+            <output name="out_fasta2" value="out1.align2.fasta" ftype="fasta" />    
+            <output name="out_fasta3" value="out1.align3.fasta" ftype="fasta" />    
+        </test>
+        <test expect_num_outputs="4">
+            <param name="genome" value="reference1.fasta" ftype="fasta" />
+            <param name="tracefile" value="input1.ab1" ftype="ab1" />
+            <param name="callVariants" value="true" />
+            <output name="out_fasta1" value="out1.align1.fasta" ftype="fasta" />    
+            <output name="out_fasta2" value="out1.align2.fasta" ftype="fasta" />    
+            <output name="out_fasta3" value="out1.align3.fasta" ftype="fasta" />    
+            <output name="out_bcf" compare="sim_size" value="out1.bcf" ftype="bcf" />
+        </test>
+        <test expect_num_outputs="5">
+            <param name="genome" value="reference1.fasta" ftype="fasta" />
+            <param name="tracefile" value="input1.ab1" ftype="ab1" />
+            <param name="callVariants" value="true" />
+            <param name="optional_outputs" value="json" />
+            <output name="out_json" ftype="json">
+                <assert_contents>
+                    <has_text text='"peakA": [5, 5, 5, 5, 5, 4' />
+                </assert_contents>
+            </output>
+            <output name="out_fasta1" value="out1.align1.fasta" ftype="fasta" />    
+            <output name="out_fasta2" value="out1.align2.fasta" ftype="fasta" />    
+            <output name="out_fasta3" value="out1.align3.fasta" ftype="fasta" />    
+            <output name="out_bcf" compare="sim_size" value="out1.bcf" ftype="bcf" />
+        </test>
+        <test expect_num_outputs="5">
+            <param name="genome" value="reference1.fasta" ftype="fasta" />
+            <param name="tracefile" value="input1.ab1" ftype="ab1" />
+            <param name="callVariants" value="true" />
+            <param name="optional_outputs" value="tabular" />
+            <output name="out_stats" ftype="tabular">
+                <assert_contents>
+                    <has_text_matching expression="13\s1\s2\s2\s11\s2\sT\sG\sN\s5\sY" />
+                </assert_contents>
+            </output>
+            <output name="out_fasta1" value="out1.align1.fasta" ftype="fasta" />    
+            <output name="out_fasta2" value="out1.align2.fasta" ftype="fasta" />    
+            <output name="out_fasta3" value="out1.align3.fasta" ftype="fasta" />    
+            <output name="out_bcf" compare="sim_size" value="out1.bcf" ftype="bcf" />
+        </test>
+    </tests>
+    <help><![CDATA[
+**What this does**
+
+Double-peaks in a trace file can cause alignment issues. This tool uses tracy_ to decompose 
+heterozygous variants in a trace file into two separate alleles. Each allele is then aligned
+separately to the reference sequence.
+
+Optionally, variants between the trace file and the reference are also output in BCF format.
+
+@pratio@
+
+@trim_options@
+
+@alignment@
+
+Read more here_.
+
+.. _tracy: https://github.com/gear-genomics/tracy
+.. _here: https://www.gear-genomics.com/docs/tracy/cli/#deconvolution-of-heterozygous-mutations
+    ]]></help>
+    <expand macro="citations" />
+</tool>
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