Mercurial > repos > iuc > trinity_super_transcripts
diff super_transcripts.xml @ 0:64094e19077d draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit 9dcdb5348f7439d0ce1e44ed23ad226beb4ae6d4
author | iuc |
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date | Thu, 14 Jun 2018 03:12:34 -0400 |
parents | |
children | b34de8dc0f64 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/super_transcripts.xml Thu Jun 14 03:12:34 2018 -0400 @@ -0,0 +1,35 @@ +<tool id="trinity_super_transcripts" name="Generate SuperTranscripts" version="@WRAPPER_VERSION@"> + <description>from a Trinity assembly</description> + <macros> + <import>macros.xml</import> + </macros> + <expand macro="requirements"/> + <command detect_errors="aggressive"><![CDATA[ + Trinity_gene_splice_modeler.py --incl_malign --trinity_fasta '$assembly' + ]]></command> + <inputs> + <param format="fasta" name="assembly" type="data" label="Trinity assembly"/> + </inputs> + <outputs> + <data format="fasta" name="seqs" from_work_dir="trinity_genes.fasta" label="${tool.name} on ${on_string}: SuperTranscripts sequences"/> + <data format="gtf" name="annotation" from_work_dir="trinity_genes.gtf" label="${tool.name} on ${on_string}: SuperTranscripts strcture annotation"/> + <data format="clustal" name="malign" from_work_dir="trinity_genes.malign" label="${tool.name} on ${on_string}: multiple alignment of SuperTranscripts"/> + </outputs> + <tests> + <test> + <param name="assembly" value="raw/Trinity.fasta" ftype="fasta"/> + <output name="seqs" file="supertranscripts/trinity_genes.fasta" /> + <output name="annotation" file="supertranscripts/trinity_genes.gtf" /> + <output name="malign" file="supertranscripts/trinity_genes.malign" /> + </test> + </tests> + <help> + SuperTranscripts provide a gene-like view of the transcriptional complexity of a gene. SuperTranscripts were originally defined by Nadia Davidson, Anthony Hawkins, and Alicia Oshlack as described in their publication "SuperTranscripts: a data driven reference for analysis and visualisation of transcriptomes" Genome Biology, 2017. SuperTranscripts are useful in the context of genome-free de novo transcriptome assembly in that they provide a genome-like reference for studying aspects of the gene including differential transcript usage (aka. differential exon usage) and as a substrate for mapping reads and identifying allelic polymorphisms. + + A SuperTranscript is constructed by collapsing unique and common sequence regions among splicing isoforms into a single linear sequence. + + .. _Trinity: http://trinityrnaseq.github.io + </help> + + <expand macro="citation" /> +</tool>