comparison macros.xml @ 16:c5a2ee07c4e1 draft default tip

planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/umi_tools commit bc1b362f6783d3fc0ed0f42c14687001d7ff5f7a

15:04e09969d376

<macros>

    <!-- macros applying to all umi_tools -->

    <token name="@TOOL_VERSION@">1.1.5</token>
    <token name="@VERSION_SUFFIX@">0</token>
    <token name="@PROFILE@">21.01</token>
    <xml name="requirements">
        <requirements>
            <requirement type="package" version="@TOOL_VERSION@">umi_tools</requirement>
            <yield />
        </requirements>

            <when value="single">
                <param name="input_read1" type="data" format="@FASTQ_FORMATS@" label="Reads in FASTQ format" />
                <expand macro="barcode1_macro"/>
            </when>
            <when value="paired">
                <param name="input_read1" type="data" format="@FASTQ_FORMATS@" label="Reads in FASTQ format" />
                <param name="input_read2" type="data" format="@FASTQ_FORMATS@" label="Reads in FASTQ format" />
                <expand macro="barcode1_macro"/>
                <expand macro="barcode2_macro"/>
                <yield/>
            </when>
            <when value="paired_collection">

    <!-- macros for count, dedup, and group -->

    <token name="@LINK_SAM_BAM_INPUT@"><![CDATA[
        #if $input.is_of_type("sam"):
            ## TODO dedup has problems with SAM input in some cases
            ## https://github.com/CGATOxford/UMI-tools/issues/483
            ## so convert it to sorted BAM for now
            ## #set $input_file = $input
            samtools sort --no-PG '$input' > 'input.bam' &&
            samtools index -b 'input.bam' &&
            #set $input_file = 'input.bam'
        #else:
            ln -sf '${input}' 'input.bam' &&
            ln -sf '$input.metadata.bam_index' 'input.bam.bai' &&
            #set $input_file = 'input.bam'
        #end if
    ]]></token>
    <token name="@SET_INPUT_TYPE@"><![CDATA[
        ## TODO see comment in LINK_SAM_BAM_INPUT
        ## #if $input.is_of_type("sam"):
        ##     --in-sam
        ## #end if
    ]]></token>

                <expand macro="sanitize_tag" />
            </param>
            <param argument="--assigned-status-tag" type="text" optional="true" label="Bam tag describing whether read is assigned to a gene" help="By default, this is set as the same tag as --gene-tag">
                <expand macro="sanitize_tag" />
            </param>
            <param argument="--skip-tags-regex" name="skip_tags_regex" type="text" label="Skip any reads where the gene matches this tag" value="" >
                <expand macro="barcode_sanitizer" />
            </param>
            <param argument="--per-contig" type="boolean" truevalue="--per-contig" falsevalue="" label="Deduplicate per contig" help="Field 3 in BAM; RNAME. All reads with the same contig will be considered to have the same alignment position. This is useful if your library prep generates PCR duplicates with non identical alignment positions such as CEL-Seq. In this case, you would align to a reference transcriptome with one transcript per gene" />
            <param argument="--gene-transcript-map" type="data" format="tabular" optional="true" label="Tabular file mapping genes to transripts" />
            <param argument="--per-cell" name="per_cell" type="boolean" truevalue="--per-cell" falsevalue="" label="Group reads only if they have the same cell barcode" />
        </section>
    </xml>
    <token name="@SC_OPTIONS@"><![CDATA[
            #if str($sc.gene_tag) != "":
                --gene-tag '$sc.gene_tag'

16:c5a2ee07c4e1

<macros>

    <!-- macros applying to all umi_tools -->

    <token name="@TOOL_VERSION@">1.1.5</token>
    <token name="@VERSION_SUFFIX@">1</token>
    <token name="@PROFILE@">23.1</token>
    <xml name="requirements">
        <requirements>
            <requirement type="package" version="@TOOL_VERSION@">umi_tools</requirement>
            <yield />
        </requirements>

            <when value="single">
                <param name="input_read1" type="data" format="@FASTQ_FORMATS@" label="Reads in FASTQ format" />
                <expand macro="barcode1_macro"/>
            </when>
            <when value="paired">
                <param name="input_read1" type="data" format="@FASTQ_FORMATS@" label="Forward reads in FASTQ format" />
                <param name="input_read2" type="data" format="@FASTQ_FORMATS@" label="Reverse reads in FASTQ format" />
                <expand macro="barcode1_macro"/>
                <expand macro="barcode2_macro"/>
                <yield/>
            </when>
            <when value="paired_collection">

    <!-- macros for count, dedup, and group -->

    <token name="@LINK_SAM_BAM_INPUT@"><![CDATA[
        #if $input.is_of_type("sam"):
            ## sam input is not supported for paired data
            ## https://github.com/CGATOxford/UMI-tools/issues/483
            ## so convert it to sorted BAM
            ## #set $input_file = $input
            samtools sort --no-PG '$input' -O BAM > 'input.bam' &&
            samtools index -b 'input.bam' &&
            #set $input_file = 'input.bam'
        #else:
            ln -sf '${input}' 'input.bam' &&
            ln -sf '$input.metadata.bam_index' 'input.bam.bai' &&
            #set $input_file = 'input.bam'
        #end if
    ]]></token>
    <token name="@SET_INPUT_TYPE@"><![CDATA[
        ## see comment in LINK_SAM_BAM_INPUT
        ## #if $input.is_of_type("sam"):
        ##     --in-sam
        ## #end if
    ]]></token>

                <expand macro="sanitize_tag" />
            </param>
            <param argument="--assigned-status-tag" type="text" optional="true" label="Bam tag describing whether read is assigned to a gene" help="By default, this is set as the same tag as --gene-tag">
                <expand macro="sanitize_tag" />
            </param>
            <param argument="--skip-tags-regex" type="text" label="Skip any reads where the gene matches this tag" value="" >
                <expand macro="barcode_sanitizer" />
            </param>
            <param argument="--per-contig" type="boolean" truevalue="--per-contig" falsevalue="" label="Deduplicate per contig" help="Field 3 in BAM; RNAME. All reads with the same contig will be considered to have the same alignment position. This is useful if your library prep generates PCR duplicates with non identical alignment positions such as CEL-Seq. In this case, you would align to a reference transcriptome with one transcript per gene" />
            <param argument="--gene-transcript-map" type="data" format="tabular" optional="true" label="Tabular file mapping genes to transripts" />
            <param argument="--per-cell" type="boolean" truevalue="--per-cell" falsevalue="" label="Group reads only if they have the same cell barcode" />
        </section>
    </xml>
    <token name="@SC_OPTIONS@"><![CDATA[
            #if str($sc.gene_tag) != "":
                --gene-tag '$sc.gene_tag'