changeset 0:a6477bafd522 draft

planemo upload commit eea727c3bdfe36d9d16036d5ab79fb8b27c4e82e
author iuc
date Wed, 10 Jan 2018 19:09:42 -0500
parents
children c0691d1977dd
files macros.xml test-data/dedup_out1.bam test-data/dedup_out2.bam test-data/dedup_out3.bam test-data/dedup_out4.bam test-data/dedup_out5.bam test-data/dedup_out6.bam test-data/group_in1.sam test-data/group_in2.bam test-data/group_in3.bam test-data/group_in4.bam test-data/group_in5.bam test-data/group_in6.bam test-data/group_out2.bam test-data/group_out3.bam test-data/group_out3.tab test-data/group_out4.bam test-data/group_out4.tab test-data/group_out5.bam test-data/group_out6.bam test-data/out_R1.fastq.gz test-data/out_R2.fastq.gz test-data/out_SE.fastq test-data/out_paired.log test-data/out_single.log test-data/t_R1.fastq test-data/t_R1.fastq.gz test-data/t_R2.fastq.gz umi-tools_dedup.xml
diffstat 28 files changed, 2242 insertions(+), 0 deletions(-) [+]
line wrap: on
line diff
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/macros.xml	Wed Jan 10 19:09:42 2018 -0500
@@ -0,0 +1,23 @@
+<?xml version="1.0"?>
+<macros>
+    <xml name="citations">
+        <citations>
+            <citation type="doi">10.1101/gr.209601.116</citation>
+            <citation type="bibtex">
+                @misc{githubUMI-tools,
+                title = {UMI-tools},
+                publisher = {GitHub},
+                journal = {GitHub repository},
+                url = {https://github.com/CGATOxford/UMI-tools},
+                }
+            </citation>
+        </citations>
+    </xml>
+    <xml name="requirements">
+        <requirements>
+            <requirement type="package" version="@VERSION@">umi_tools</requirement>
+            <yield />
+        </requirements>
+    </xml>
+    <token name="@VERSION@">0.5.3</token>
+</macros>
Binary file test-data/dedup_out1.bam has changed
Binary file test-data/dedup_out2.bam has changed
Binary file test-data/dedup_out3.bam has changed
Binary file test-data/dedup_out4.bam has changed
Binary file test-data/dedup_out5.bam has changed
Binary file test-data/dedup_out6.bam has changed
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/group_in1.sam	Wed Jan 10 19:09:42 2018 -0500
@@ -0,0 +1,666 @@
+@HD	VN:1.0	SO:coordinate
+@PG	ID:Bowtie	VN:1.1.2	CL:"bowtie --wrapper basic-0 --threads 4 -v 2 -m 10 -a /ifs/mirror/genomes/bowtie/mm9 /dev/fd/63 --sam"
+@SQ	SN:chr10	LN:129993255
+@SQ	SN:chr11	LN:121843856
+@SQ	SN:chr12	LN:121257530
+@SQ	SN:chr13	LN:120284312
+@SQ	SN:chr14	LN:125194864
+@SQ	SN:chr15	LN:103494974
+@SQ	SN:chr16	LN:98319150
+@SQ	SN:chr17	LN:95272651
+@SQ	SN:chr18	LN:90772031
+@SQ	SN:chr19	LN:61342430
+@SQ	SN:chr1	LN:197195432
+@SQ	SN:chr2	LN:181748087
+@SQ	SN:chr3	LN:159599783
+@SQ	SN:chr4	LN:155630120
+@SQ	SN:chr5	LN:152537259
+@SQ	SN:chr6	LN:149517037
+@SQ	SN:chr7	LN:152524553
+@SQ	SN:chr8	LN:131738871
+@SQ	SN:chr9	LN:124076172
+@SQ	SN:chrM	LN:16299
+@SQ	SN:chrX	LN:166650296
+@SQ	SN:chrY	LN:15902555
+SRR2057595.10000392_AAGTA	16	chr19	31326011	255	57M	*	0	0	*	*	XA:i:1	MD:Z:23A33	NM:i:1	UG:i:512	BX:Z:AAGTA
+SRR2057595.10000468_GCTAT	16	chr19	61240267	255	28M	*	0	0	*	*	XA:i:1	MD:Z:11C16	NM:i:1	UG:i:706	BX:Z:GCTAT
+SRR2057595.10000704_TGATG	16	chr19	3544146	255	39M	*	0	0	*	*	XA:i:0	MD:Z:39	NM:i:0	UG:i:1	BX:Z:TGATG
+SRR2057595.10000838_TAGTA	0	chr19	10274608	255	34M	*	0	0	*	*	XA:i:0	MD:Z:34	NM:i:0	UG:i:324	BX:Z:TAGTA
+SRR2057595.10001118_TCTCA	16	chr19	4078298	255	50M	*	0	0	*	*	XA:i:1	MD:Z:43A6	NM:i:1	UG:i:36	BX:Z:TCTCA
+SRR2057595.10001329_CGGTC	16	chr19	28083193	255	60M	*	0	0	*	*	XA:i:0	MD:Z:60	NM:i:0	UG:i:497	BX:Z:CGGTC
+SRR2057595.10001382_CTGCC	16	chr19	3957282	255	22M	*	0	0	*	*	XA:i:2	MD:Z:17C1G2	NM:i:2	UG:i:9	BX:Z:CTGCC
+SRR2057595.10001382_CTGCC	16	chr19	3970897	255	22M	*	0	0	*	*	XA:i:2	MD:Z:17C1G2	NM:i:2	UG:i:10	BX:Z:CTGCC
+SRR2057595.10002047_ATTAT	16	chr19	7546213	255	27M	*	0	0	*	*	XA:i:0	MD:Z:27	NM:i:0	UG:i:278	BX:Z:ATTAT
+SRR2057595.10002219_ATCAG	16	chr19	5796783	255	34M	*	0	0	*	*	XA:i:0	MD:Z:34	NM:i:0	UG:i:234	BX:Z:ATCAG
+SRR2057595.10002597_AATTA	16	chr19	50733887	255	64M	*	0	0	*	*	XA:i:2	MD:Z:58C0C4	NM:i:2	UG:i:645	BX:Z:AATTA
+SRR2057595.10003084_GTCTA	16	chr19	4078420	255	67M	*	0	0	*	*	XA:i:2	MD:Z:2T19A44	NM:i:2	UG:i:124	BX:Z:GTCTA
+SRR2057595.10003230_ACATT	0	chr19	8799370	255	64M	*	0	0	*	*	XA:i:0	MD:Z:64	NM:i:0	UG:i:297	BX:Z:ACATT
+SRR2057595.10003317_CGGCC	16	chr19	52641725	255	20M	*	0	0	*	*	XA:i:2	MD:Z:1C13A4	NM:i:2	UG:i:648	BX:Z:CGGCC
+SRR2057595.10003567_ATGTC	0	chr19	37052811	255	64M	*	0	0	*	*	XA:i:1	MD:Z:11C52	NM:i:1	UG:i:542	BX:Z:ATGTC
+SRR2057595.10003576_AATCG	16	chr19	4078303	255	67M	*	0	0	*	*	XA:i:2	MD:Z:38A21A6	NM:i:2	UG:i:88	BX:Z:AATCG
+SRR2057595.10003630_GTGTT	16	chr19	4078298	255	52M	*	0	0	*	*	XA:i:2	MD:Z:42G0A8	NM:i:2	UG:i:45	BX:Z:GTGTT
+SRR2057595.10003869_TCTTG	16	chr19	4078423	255	67M	*	0	0	*	*	XA:i:1	MD:Z:19A47	NM:i:1	UG:i:111	BX:Z:TCTTG
+SRR2057595.1000404_AACTT	16	chr19	4078420	255	64M	*	0	0	*	*	XA:i:2	MD:Z:2T19A41	NM:i:2	UG:i:55	BX:Z:AACTT
+SRR2057595.10004161_TTCGC	16	chr19	4078428	255	67M	*	0	0	*	*	XA:i:2	MD:Z:14A24T27	NM:i:2	UG:i:74	BX:Z:TTCGC
+SRR2057595.1000475_TCTGG	0	chr19	13129234	255	28M	*	0	0	*	*	XA:i:1	MD:Z:22T5	NM:i:1	UG:i:383	BX:Z:TCTGG
+SRR2057595.10004845_GGATA	0	chr19	24394180	255	29M	*	0	0	*	*	XA:i:2	MD:Z:11C15T1	NM:i:2	UG:i:481	BX:Z:GGATA
+SRR2057595.10005100_TTAAA	16	chr19	4785463	255	21M	*	0	0	*	*	XA:i:0	MD:Z:21	NM:i:0	UG:i:196	BX:Z:TTAAA
+SRR2057595.10005761_CTAGA	16	chr19	4078299	255	50M	*	0	0	*	*	XA:i:2	MD:Z:41G0A7	NM:i:2	UG:i:43	BX:Z:CTAGA
+SRR2057595.10005808_CAGTA	16	chr19	7545872	255	24M	*	0	0	*	*	XA:i:0	MD:Z:24	NM:i:0	UG:i:276	BX:Z:CAGTA
+SRR2057595.10005955_GACAT	16	chr19	4078302	255	67M	*	0	0	*	*	XA:i:2	MD:Z:39A21A5	NM:i:2	UG:i:82	BX:Z:GACAT
+SRR2057595.10006011_TCTCA	16	chr19	4078298	255	50M	*	0	0	*	*	XA:i:1	MD:Z:43A6	NM:i:1	UG:i:36	BX:Z:TCTCA
+SRR2057595.10006546_GCTAC	16	chr19	4078446	255	67M	*	0	0	*	*	XA:i:0	MD:Z:67	NM:i:0	UG:i:97	BX:Z:GCTAC
+SRR2057595.1000663_ACGCA	16	chr19	51217013	255	67M	*	0	0	*	*	XA:i:2	MD:Z:3A46A16	NM:i:2	UG:i:647	BX:Z:ACGCA
+SRR2057595.10006870_GTCCA	16	chr19	4078431	255	67M	*	0	0	*	*	XA:i:1	MD:Z:11A55	NM:i:1	UG:i:107	BX:Z:GTCCA
+SRR2057595.10006926_GGATA	0	chr19	24394180	255	29M	*	0	0	*	*	XA:i:2	MD:Z:11C15T1	NM:i:2	UG:i:481	BX:Z:GGATA
+SRR2057595.10006986_GCGGA	16	chr19	4078298	255	48M	*	0	0	*	*	XA:i:1	MD:Z:43A4	NM:i:1	UG:i:19	BX:Z:GCGGA
+SRR2057595.10007554_TATTG	0	chr19	13129322	255	28M	*	0	0	*	*	XA:i:0	MD:Z:28	NM:i:0	UG:i:404	BX:Z:TATTG
+SRR2057595.10008217_GCTAT	0	chr19	57701750	255	67M	*	0	0	*	*	XA:i:2	MD:Z:34T1T30	NM:i:2	UG:i:677	BX:Z:GCTAT
+SRR2057595.10008433_AGAGT	16	chr19	24180225	255	55M	*	0	0	*	*	XA:i:1	MD:Z:41C13	NM:i:1	UG:i:474	BX:Z:AGAGT
+SRR2057595.10008484_GGATC	16	chr19	4078298	255	61M	*	0	0	*	*	XA:i:2	MD:Z:42G0A17	NM:i:2	UG:i:61	BX:Z:GGATC
+SRR2057595.10008523_TATGG	0	chr19	13129322	255	56M	*	0	0	*	*	XA:i:2	MD:Z:33C5A16	NM:i:2	UG:i:405	BX:Z:TATGG
+SRR2057595.10008761_AGCTG	16	chr19	4806926	255	39M	*	0	0	*	*	XA:i:0	MD:Z:39	NM:i:0	UG:i:201	BX:Z:AGCTG
+SRR2057595.10009130_CGGCC	16	chr19	52641725	255	20M	*	0	0	*	*	XA:i:2	MD:Z:1C13A4	NM:i:2	UG:i:648	BX:Z:CGGCC
+SRR2057595.10009213_CCCCG	16	chr19	4078298	255	38M	*	0	0	*	*	XA:i:1	MD:Z:35C2	NM:i:1	UG:i:184	BX:Z:CCCCG
+SRR2057595.10009394_TAGAT	16	chr19	5797239	255	48M	*	0	0	*	*	XA:i:0	MD:Z:48	NM:i:0	UG:i:229	BX:Z:TAGAT
+SRR2057595.10009607_TTGCA	0	chr19	41640185	255	61M	*	0	0	*	*	XA:i:0	MD:Z:61	NM:i:0	UG:i:581	BX:Z:TTGCA
+SRR2057595.1001008_TATCA	16	chr19	60837460	255	67M	*	0	0	*	*	XA:i:0	MD:Z:67	NM:i:0	UG:i:691	BX:Z:TATCA
+SRR2057595.10010444_AGTGC	0	chr19	8798525	255	27M	*	0	0	*	*	XA:i:0	MD:Z:27	NM:i:0	UG:i:296	BX:Z:AGTGC
+SRR2057595.10011106_CGGTC	16	chr19	28083193	255	60M	*	0	0	*	*	XA:i:0	MD:Z:60	NM:i:0	UG:i:497	BX:Z:CGGTC
+SRR2057595.10011603_ATGAA	0	chr19	56177103	255	20M	*	0	0	*	*	XA:i:2	MD:Z:12G0T6	NM:i:2	UG:i:667	BX:Z:ATGAA
+SRR2057595.10011612_GTTTC	0	chr19	58015463	255	56M	*	0	0	*	*	XA:i:0	MD:Z:56	NM:i:0	UG:i:683	BX:Z:GTTTC
+SRR2057595.10011924_CCCCG	16	chr19	4078298	255	38M	*	0	0	*	*	XA:i:0	MD:Z:38	NM:i:0	UG:i:184	BX:Z:CCCCG
+SRR2057595.10012143_ATGAA	0	chr19	56007800	255	67M	*	0	0	*	*	XA:i:0	MD:Z:67	NM:i:0	UG:i:666	BX:Z:ATGAA
+SRR2057595.10012487_TGGGT	16	chr19	4917801	255	27M	*	0	0	*	*	XA:i:0	MD:Z:27	NM:i:0	UG:i:202	BX:Z:TGGGT
+SRR2057595.1001256_TGATG	16	chr19	3544146	255	39M	*	0	0	*	*	XA:i:0	MD:Z:39	NM:i:0	UG:i:1	BX:Z:TGATG
+SRR2057595.10013018_GGGGC	16	chr19	4078446	255	67M	*	0	0	*	*	XA:i:1	MD:Z:66T0	NM:i:1	UG:i:98	BX:Z:GGGGC
+SRR2057595.10013156_GTCAA	16	chr19	4078298	255	57M	*	0	0	*	*	XA:i:1	MD:Z:43A13	NM:i:1	UG:i:52	BX:Z:GTCAA
+SRR2057595.10013642_AATTC	0	chr19	13129322	255	27M	*	0	0	*	*	XA:i:0	MD:Z:27	NM:i:0	UG:i:403	BX:Z:AATTC
+SRR2057595.10013703_ATGTA	0	chr19	39042685	255	39M	*	0	0	*	*	XA:i:0	MD:Z:39	NM:i:0	UG:i:558	BX:Z:ATGTA
+SRR2057595.10014121_ATACC	16	chr19	35673152	255	21M	*	0	0	*	*	XA:i:2	MD:Z:7T2A10	NM:i:2	UG:i:534	BX:Z:ATACC
+SRR2057595.1001419_CAGTA	16	chr19	7545872	255	24M	*	0	0	*	*	XA:i:0	MD:Z:24	NM:i:0	UG:i:276	BX:Z:CAGTA
+SRR2057595.10014588_AAGGT	0	chr19	8798514	255	39M	*	0	0	*	*	XA:i:0	MD:Z:39	NM:i:0	UG:i:294	BX:Z:AAGGT
+SRR2057595.10014904_GCGAC	0	chr19	13129553	255	26M	*	0	0	*	*	XA:i:2	MD:Z:3A0T21	NM:i:2	UG:i:399	BX:Z:GCGAC
+SRR2057595.10015153_ATTAT	16	chr19	7546213	255	27M	*	0	0	*	*	XA:i:0	MD:Z:27	NM:i:0	UG:i:278	BX:Z:ATTAT
+SRR2057595.10015867_ATCCG	16	chr19	37369602	255	35M	*	0	0	*	*	XA:i:0	MD:Z:35	NM:i:0	UG:i:544	BX:Z:ATCCG
+SRR2057595.10015949_CTTTC	16	chr19	4078298	255	53M	*	0	0	*	*	XA:i:2	MD:Z:42G0A9	NM:i:2	UG:i:47	BX:Z:CTTTC
+SRR2057595.10016080_TATCA	16	chr19	60837460	255	67M	*	0	0	*	*	XA:i:0	MD:Z:67	NM:i:0	UG:i:691	BX:Z:TATCA
+SRR2057595.10016534_ACGCA	16	chr19	51217013	255	67M	*	0	0	*	*	XA:i:2	MD:Z:3A46A16	NM:i:2	UG:i:647	BX:Z:ACGCA
+SRR2057595.10016692_TGGAC	16	chr19	4078420	255	67M	*	0	0	*	*	XA:i:2	MD:Z:2T19A44	NM:i:2	UG:i:127	BX:Z:TGGAC
+SRR2057595.10016737_GTTGT	16	chr19	12564367	255	21M	*	0	0	*	*	XA:i:2	MD:Z:0A12A7	NM:i:2	UG:i:380	BX:Z:GTTGT
+SRR2057595.10016874_TTGCA	0	chr19	44434953	255	43M	*	0	0	*	*	XA:i:0	MD:Z:43	NM:i:0	UG:i:602	BX:Z:TTGCA
+SRR2057595.10017358_AGATC	16	chr19	24352786	255	67M	*	0	0	*	*	XA:i:1	MD:Z:25C41	NM:i:1	UG:i:479	BX:Z:AGATC
+SRR2057595.10017433_GGCAG	0	chr19	13129322	255	22M	*	0	0	*	*	XA:i:0	MD:Z:22	NM:i:0	UG:i:409	BX:Z:GGCAG
+SRR2057595.10017742_TAGCG	0	chr19	25429456	255	20M	*	0	0	*	*	XA:i:2	MD:Z:5A9A4	NM:i:2	UG:i:485	BX:Z:TAGCG
+SRR2057595.10018091_TAACA	0	chr19	8799023	255	67M	*	0	0	*	*	XA:i:0	MD:Z:67	NM:i:0	UG:i:299	BX:Z:TAACA
+SRR2057595.10018143_CCATC	0	chr19	5008676	255	30M	*	0	0	*	*	XA:i:2	MD:Z:1A23G4	NM:i:2	UG:i:203	BX:Z:CCATC
+SRR2057595.10018237_GCTTG	16	chr19	5797471	255	50M	*	0	0	*	*	XA:i:0	MD:Z:50	NM:i:0	UG:i:226	BX:Z:GCTTG
+SRR2057595.10018343_AATTC	0	chr19	13129322	255	27M	*	0	0	*	*	XA:i:0	MD:Z:27	NM:i:0	UG:i:403	BX:Z:AATTC
+SRR2057595.10018510_TTCGT	0	chr19	47167097	255	67M	*	0	0	*	*	XA:i:0	MD:Z:67	NM:i:0	UG:i:623	BX:Z:TTCGT
+SRR2057595.10018668_GAGAG	16	chr19	4078298	255	50M	*	0	0	*	*	XA:i:1	MD:Z:43A6	NM:i:1	UG:i:35	BX:Z:GAGAG
+SRR2057595.10018780_CTAGA	16	chr19	4078299	255	50M	*	0	0	*	*	XA:i:2	MD:Z:41G0A7	NM:i:2	UG:i:43	BX:Z:CTAGA
+SRR2057595.10018930_TGATT	16	chr19	4078299	255	50M	*	0	0	*	*	XA:i:2	MD:Z:41G0A7	NM:i:2	UG:i:41	BX:Z:TGATT
+SRR2057595.10019300_TTATA	16	chr19	4078298	255	49M	*	0	0	*	*	XA:i:2	MD:Z:42G0A5	NM:i:2	UG:i:22	BX:Z:TTATA
+SRR2057595.10019327_TTATA	16	chr19	4078298	255	49M	*	0	0	*	*	XA:i:2	MD:Z:42G0A5	NM:i:2	UG:i:22	BX:Z:TTATA
+SRR2057595.10019367_AGTAT	0	chr19	10600420	255	29M	*	0	0	*	*	XA:i:0	MD:Z:29	NM:i:0	UG:i:328	BX:Z:AGTAT
+SRR2057595.10019428_CTAAT	0	chr19	40194243	255	44M	*	0	0	*	*	XA:i:2	MD:Z:5T20A17	NM:i:2	UG:i:562	BX:Z:CTAAT
+SRR2057595.10019544_CCCCG	16	chr19	4078298	255	38M	*	0	0	*	*	XA:i:0	MD:Z:38	NM:i:0	UG:i:184	BX:Z:CCCCG
+SRR2057595.100195_TTCGC	16	chr19	4078428	255	67M	*	0	0	*	*	XA:i:2	MD:Z:14A24T27	NM:i:2	UG:i:74	BX:Z:TTCGC
+SRR2057595.10019604_TCCAG	0	chr19	29662201	255	26M	*	0	0	*	*	XA:i:1	MD:Z:0C25	NM:i:1	UG:i:509	BX:Z:TCCAG
+SRR2057595.10019935_ACCGT	16	chr19	15980484	255	29M	*	0	0	*	*	XA:i:1	MD:Z:12C16	NM:i:1	UG:i:431	BX:Z:ACCGT
+SRR2057595.10020101_CTAGA	16	chr19	4078299	255	50M	*	0	0	*	*	XA:i:2	MD:Z:41G0A7	NM:i:2	UG:i:43	BX:Z:CTAGA
+SRR2057595.10020134_CTTTC	16	chr19	4078298	255	45M	*	0	0	*	*	XA:i:1	MD:Z:43A1	NM:i:1	UG:i:15	BX:Z:CTTTC
+SRR2057595.10020463_GTCCT	16	chr19	59694502	255	21M	*	0	0	*	*	XA:i:2	MD:Z:8T0A11	NM:i:2	UG:i:688	BX:Z:GTCCT
+SRR2057595.10020843_TTAAA	16	chr19	4785463	255	21M	*	0	0	*	*	XA:i:0	MD:Z:21	NM:i:0	UG:i:196	BX:Z:TTAAA
+SRR2057595.10020890_AGTAT	0	chr19	10600420	255	29M	*	0	0	*	*	XA:i:0	MD:Z:29	NM:i:0	UG:i:328	BX:Z:AGTAT
+SRR2057595.10021356_CAGTA	16	chr19	61240266	255	26M	*	0	0	*	*	XA:i:1	MD:Z:12C13	NM:i:1	UG:i:697	BX:Z:CAGTA
+SRR2057595.10021429_CACTG	16	chr19	5798673	255	42M	*	0	0	*	*	XA:i:0	MD:Z:42	NM:i:0	UG:i:249	BX:Z:CACTG
+SRR2057595.10021850_AGTCT	0	chr19	13129558	255	37M	*	0	0	*	*	XA:i:1	MD:Z:21A15	NM:i:1	UG:i:398	BX:Z:AGTCT
+SRR2057595.10022647_TTGTT	16	chr19	61240268	255	24M	*	0	0	*	*	XA:i:1	MD:Z:10C13	NM:i:1	UG:i:696	BX:Z:TTGTT
+SRR2057595.10022675_GTGGA	16	chr19	35902240	255	30M	*	0	0	*	*	XA:i:0	MD:Z:30	NM:i:0	UG:i:535	BX:Z:GTGGA
+SRR2057595.10023366_AGCTT	16	chr19	5799262	255	20M	*	0	0	*	*	XA:i:0	MD:Z:20	NM:i:0	UG:i:245	BX:Z:AGCTT
+SRR2057595.10023594_ATGAA	0	chr19	56007800	255	67M	*	0	0	*	*	XA:i:0	MD:Z:67	NM:i:0	UG:i:666	BX:Z:ATGAA
+SRR2057595.10023941_TACGG	16	chr19	4078296	255	67M	*	0	0	*	*	XA:i:2	MD:Z:1T43A21	NM:i:2	UG:i:71	BX:Z:TACGG
+SRR2057595.10023975_TCGTC	16	chr19	61240267	255	28M	*	0	0	*	*	XA:i:1	MD:Z:11C16	NM:i:1	UG:i:704	BX:Z:TCGTC
+SRR2057595.10024016_TTTTC	16	chr19	4078296	255	67M	*	0	0	*	*	XA:i:2	MD:Z:0T44A21	NM:i:2	UG:i:69	BX:Z:TTTTC
+SRR2057595.10024824_ACCGG	16	chr19	10924297	255	49M	*	0	0	*	*	XA:i:0	MD:Z:49	NM:i:0	UG:i:336	BX:Z:ACCGG
+SRR2057595.10025477_AGTAT	0	chr19	10600420	255	29M	*	0	0	*	*	XA:i:0	MD:Z:29	NM:i:0	UG:i:328	BX:Z:AGTAT
+SRR2057595.10025641_CGAAC	0	chr19	13129273	255	36M	*	0	0	*	*	XA:i:2	MD:Z:25C1T8	NM:i:2	UG:i:386	BX:Z:CGAAC
+SRR2057595.10025783_GCATA	16	chr19	4078298	255	57M	*	0	0	*	*	XA:i:2	MD:Z:42G0A13	NM:i:2	UG:i:53	BX:Z:GCATA
+SRR2057595.10025842_GATCT	16	chr19	4078433	255	67M	*	0	0	*	*	XA:i:1	MD:Z:9A57	NM:i:1	UG:i:90	BX:Z:GATCT
+SRR2057595.10026676_GGATC	16	chr19	4078298	255	61M	*	0	0	*	*	XA:i:2	MD:Z:42G0A17	NM:i:2	UG:i:61	BX:Z:GGATC
+SRR2057595.1002669_TATTC	16	chr19	5038295	255	24M	*	0	0	*	*	XA:i:0	MD:Z:24	NM:i:0	UG:i:206	BX:Z:TATTC
+SRR2057595.10027010_TGTTT	16	chr19	50526830	255	51M	*	0	0	*	*	XA:i:0	MD:Z:51	NM:i:0	UG:i:643	BX:Z:TGTTT
+SRR2057595.10027016_GACGT	0	chr19	40194275	255	30M	*	0	0	*	*	XA:i:0	MD:Z:30	NM:i:0	UG:i:565	BX:Z:GACGT
+SRR2057595.10027043_TATGG	0	chr19	13129322	255	56M	*	0	0	*	*	XA:i:2	MD:Z:33C5A16	NM:i:2	UG:i:405	BX:Z:TATGG
+SRR2057595.10027109_GGGGC	16	chr19	4078446	255	67M	*	0	0	*	*	XA:i:1	MD:Z:66T0	NM:i:1	UG:i:98	BX:Z:GGGGC
+SRR2057595.10027286_ATGAA	0	chr19	56177103	255	20M	*	0	0	*	*	XA:i:2	MD:Z:12G0T6	NM:i:2	UG:i:667	BX:Z:ATGAA
+SRR2057595.10027736_CCGAT	16	chr19	4078300	255	49M	*	0	0	*	*	XA:i:2	MD:Z:40G0A7	NM:i:2	UG:i:44	BX:Z:CCGAT
+SRR2057595.10027811_AAAGG	16	chr19	23347776	255	34M	*	0	0	*	*	XA:i:1	MD:Z:9T24	NM:i:1	UG:i:467	BX:Z:AAAGG
+SRR2057595.10028205_GCTAT	0	chr19	57701750	255	67M	*	0	0	*	*	XA:i:2	MD:Z:34T1T30	NM:i:2	UG:i:677	BX:Z:GCTAT
+SRR2057595.10028311_GCTAT	0	chr19	57701750	255	67M	*	0	0	*	*	XA:i:2	MD:Z:34T1T30	NM:i:2	UG:i:677	BX:Z:GCTAT
+SRR2057595.10029121_AGCCG	0	chr19	8799400	255	33M	*	0	0	*	*	XA:i:0	MD:Z:33	NM:i:0	UG:i:302	BX:Z:AGCCG
+SRR2057595.10029169_GGTTC	16	chr19	56896659	255	23M	*	0	0	*	*	XA:i:2	MD:Z:0C0A21	NM:i:2	UG:i:674	BX:Z:GGTTC
+SRR2057595.10029364_GCTAT	0	chr19	57701750	255	67M	*	0	0	*	*	XA:i:2	MD:Z:34T1T30	NM:i:2	UG:i:677	BX:Z:GCTAT
+SRR2057595.10029373_TTTTC	16	chr19	4078296	255	67M	*	0	0	*	*	XA:i:2	MD:Z:0T44A21	NM:i:2	UG:i:69	BX:Z:TTTTC
+SRR2057595.10029475_ACAAA	16	chr19	5688438	255	28M	*	0	0	*	*	XA:i:0	MD:Z:28	NM:i:0	UG:i:222	BX:Z:ACAAA
+SRR2057595.10029622_TCAGG	0	chr19	13129322	255	35M	*	0	0	*	*	XA:i:1	MD:Z:33C1	NM:i:1	UG:i:407	BX:Z:TCAGG
+SRR2057595.10029643_TATGG	0	chr19	13129322	255	56M	*	0	0	*	*	XA:i:2	MD:Z:33C5A16	NM:i:2	UG:i:405	BX:Z:TATGG
+SRR2057595.10030278_TATTG	0	chr19	13129322	255	28M	*	0	0	*	*	XA:i:0	MD:Z:28	NM:i:0	UG:i:404	BX:Z:TATTG
+SRR2057595.10030560_ATACC	16	chr19	35673152	255	21M	*	0	0	*	*	XA:i:2	MD:Z:7T2A10	NM:i:2	UG:i:534	BX:Z:ATACC
+SRR2057595.10030639_GCGAC	0	chr19	13129553	255	26M	*	0	0	*	*	XA:i:2	MD:Z:3A0T21	NM:i:2	UG:i:399	BX:Z:GCGAC
+SRR2057595.10031002_AAGGT	0	chr19	8798514	255	39M	*	0	0	*	*	XA:i:0	MD:Z:39	NM:i:0	UG:i:294	BX:Z:AAGGT
+SRR2057595.10031358_TAGTA	16	chr19	61240268	255	27M	*	0	0	*	*	XA:i:1	MD:Z:10C16	NM:i:1	UG:i:702	BX:Z:TAGTA
+SRR2057595.10031368_TAACA	16	chr19	4078298	255	50M	*	0	0	*	*	XA:i:2	MD:Z:42G0A6	NM:i:2	UG:i:32	BX:Z:TAACA
+SRR2057595.10032378_CAGCA	16	chr19	5801892	255	47M	*	0	0	*	*	XA:i:0	MD:Z:47	NM:i:0	UG:i:258	BX:Z:CAGCA
+SRR2057595.10032451_ACCGT	16	chr19	15980484	255	29M	*	0	0	*	*	XA:i:1	MD:Z:12C16	NM:i:1	UG:i:431	BX:Z:ACCGT
+SRR2057595.10032626_CATAA	0	chr19	13129322	255	35M	*	0	0	*	*	XA:i:1	MD:Z:33C1	NM:i:1	UG:i:401	BX:Z:CATAA
+SRR2057595.10033730_AACTG	16	chr19	40362914	255	38M	*	0	0	*	*	XA:i:0	MD:Z:38	NM:i:0	UG:i:571	BX:Z:AACTG
+SRR2057595.10034125_CTCTT	16	chr19	10761035	255	32M	*	0	0	*	*	XA:i:0	MD:Z:32	NM:i:0	UG:i:332	BX:Z:CTCTT
+SRR2057595.1003424_GGATA	0	chr19	24394180	255	29M	*	0	0	*	*	XA:i:2	MD:Z:11C15T1	NM:i:2	UG:i:481	BX:Z:GGATA
+SRR2057595.10034498_ATGAA	0	chr19	56177103	255	20M	*	0	0	*	*	XA:i:2	MD:Z:12G0T6	NM:i:2	UG:i:667	BX:Z:ATGAA
+SRR2057595.10034756_ATGAA	0	chr19	56007800	255	67M	*	0	0	*	*	XA:i:0	MD:Z:67	NM:i:0	UG:i:666	BX:Z:ATGAA
+SRR2057595.10034981_TTCGT	0	chr19	47167097	255	67M	*	0	0	*	*	XA:i:0	MD:Z:67	NM:i:0	UG:i:623	BX:Z:TTCGT
+SRR2057595.10035130_GGATG	0	chr19	13129322	255	27M	*	0	0	*	*	XA:i:0	MD:Z:27	NM:i:0	UG:i:402	BX:Z:GGATG
+SRR2057595.10035284_TACGT	0	chr19	40194308	255	34M	*	0	0	*	*	XA:i:1	MD:Z:3A30	NM:i:1	UG:i:563	BX:Z:TACGT
+SRR2057595.1003559_CCATA	0	chr19	13129322	255	30M	*	0	0	*	*	XA:i:0	MD:Z:30	NM:i:0	UG:i:408	BX:Z:CCATA
+SRR2057595.10035727_TTTTC	16	chr19	4078296	255	67M	*	0	0	*	*	XA:i:2	MD:Z:0T44A21	NM:i:2	UG:i:69	BX:Z:TTTTC
+SRR2057595.1003581_TAACA	16	chr19	4078298	255	50M	*	0	0	*	*	XA:i:2	MD:Z:42G0A6	NM:i:2	UG:i:32	BX:Z:TAACA
+SRR2057595.10036346_AGTCG	0	chr19	17767763	255	67M	*	0	0	*	*	XA:i:2	MD:Z:10A4A51	NM:i:2	UG:i:440	BX:Z:AGTCG
+SRR2057595.10036388_CCAAG	16	chr19	4078298	255	50M	*	0	0	*	*	XA:i:2	MD:Z:42G0A6	NM:i:2	UG:i:37	BX:Z:CCAAG
+SRR2057595.10037180_GGTAG	16	chr19	4078298	255	37M	*	0	0	*	*	XA:i:0	MD:Z:37	NM:i:0	UG:i:177	BX:Z:GGTAG
+SRR2057595.10037421_GCCGA	16	chr19	7389994	255	48M	*	0	0	*	*	XA:i:1	MD:Z:47C0	NM:i:1	UG:i:274	BX:Z:GCCGA
+SRR2057595.10037481_CATAA	0	chr19	13129322	255	35M	*	0	0	*	*	XA:i:1	MD:Z:33C1	NM:i:1	UG:i:401	BX:Z:CATAA
+SRR2057595.10037635_TTCGT	0	chr19	47167097	255	67M	*	0	0	*	*	XA:i:0	MD:Z:67	NM:i:0	UG:i:623	BX:Z:TTCGT
+SRR2057595.10037650_GATGA	0	chr19	48194137	255	59M	*	0	0	*	*	XA:i:0	MD:Z:59	NM:i:0	UG:i:635	BX:Z:GATGA
+SRR2057595.10037690_TCCCG	16	chr19	56004832	255	67M	*	0	0	*	*	XA:i:0	MD:Z:67	NM:i:0	UG:i:663	BX:Z:TCCCG
+SRR2057595.10038156_AAACA	16	chr19	4078299	255	49M	*	0	0	*	*	XA:i:1	MD:Z:42A6	NM:i:1	UG:i:33	BX:Z:AAACA
+SRR2057595.10038276_TAGTA	0	chr19	10274608	255	34M	*	0	0	*	*	XA:i:0	MD:Z:34	NM:i:0	UG:i:324	BX:Z:TAGTA
+SRR2057595.10038572_TGTTT	0	chr19	41595518	255	38M	*	0	0	*	*	XA:i:1	MD:Z:25G12	NM:i:1	UG:i:579	BX:Z:TGTTT
+SRR2057595.10038660_CAGAT	0	chr19	13129299	255	32M	*	0	0	*	*	XA:i:2	MD:Z:1T18G11	NM:i:2	UG:i:396	BX:Z:CAGAT
+SRR2057595.1003901_AGCCG	16	chr19	4078299	255	36M	*	0	0	*	*	XA:i:0	MD:Z:36	NM:i:0	UG:i:173	BX:Z:AGCCG
+SRR2057595.1003905_CCTCA	0	chr19	8798505	255	48M	*	0	0	*	*	XA:i:0	MD:Z:48	NM:i:0	UG:i:292	BX:Z:CCTCA
+SRR2057595.10039161_CTTAC	16	chr19	4078298	255	54M	*	0	0	*	*	XA:i:2	MD:Z:42G0A10	NM:i:2	UG:i:49	BX:Z:CTTAC
+SRR2057595.10039249_TCTGT	16	chr19	45885126	255	37M	*	0	0	*	*	XA:i:0	MD:Z:37	NM:i:0	UG:i:608	BX:Z:TCTGT
+SRR2057595.10039623_CGCCG	16	chr19	4078299	255	59M	*	0	0	*	*	XA:i:1	MD:Z:42A16	NM:i:1	UG:i:58	BX:Z:CGCCG
+SRR2057595.1004011_GTCGC	0	chr19	12080548	255	51M	*	0	0	*	*	XA:i:2	MD:Z:28C20G1	NM:i:2	UG:i:345	BX:Z:GTCGC
+SRR2057595.1004011_GTCGC	16	chr19	12084189	255	51M	*	0	0	*	*	XA:i:2	MD:Z:1C20G28	NM:i:2	UG:i:352	BX:Z:GTCGC
+SRR2057595.10040530_CCTTG	16	chr19	4078298	255	49M	*	0	0	*	*	XA:i:1	MD:Z:43A5	NM:i:1	UG:i:24	BX:Z:CCTTG
+SRR2057595.10041125_GACGC	16	chr19	32419974	255	67M	*	0	0	*	*	XA:i:0	MD:Z:67	NM:i:0	UG:i:514	BX:Z:GACGC
+SRR2057595.10041133_ACAGA	16	chr19	4078299	255	22M	*	0	0	*	*	XA:i:2	MD:Z:0C0C20	NM:i:2	UG:i:136	BX:Z:ACAGA
+SRR2057595.10041495_TGTTT	0	chr19	41595518	255	38M	*	0	0	*	*	XA:i:1	MD:Z:25G12	NM:i:1	UG:i:579	BX:Z:TGTTT
+SRR2057595.10041520_TGACA	16	chr19	51217013	255	43M	*	0	0	*	*	XA:i:2	MD:Z:3A1C37	NM:i:2	UG:i:646	BX:Z:TGACA
+SRR2057595.10041577_GTCAA	16	chr19	4078298	255	57M	*	0	0	*	*	XA:i:1	MD:Z:43A13	NM:i:1	UG:i:52	BX:Z:GTCAA
+SRR2057595.10041629_GATGA	0	chr19	48194137	255	59M	*	0	0	*	*	XA:i:0	MD:Z:59	NM:i:0	UG:i:635	BX:Z:GATGA
+SRR2057595.10041662_GGGGC	16	chr19	4078446	255	67M	*	0	0	*	*	XA:i:1	MD:Z:66T0	NM:i:1	UG:i:98	BX:Z:GGGGC
+SRR2057595.10042115_GACAT	16	chr19	4078302	255	67M	*	0	0	*	*	XA:i:2	MD:Z:39A21A5	NM:i:2	UG:i:82	BX:Z:GACAT
+SRR2057595.10042422_TCTGG	0	chr19	13129234	255	28M	*	0	0	*	*	XA:i:1	MD:Z:22T5	NM:i:1	UG:i:383	BX:Z:TCTGG
+SRR2057595.1004285_CCAAC	0	chr19	8820047	255	33M	*	0	0	*	*	XA:i:0	MD:Z:33	NM:i:0	UG:i:315	BX:Z:CCAAC
+SRR2057595.10042887_AAAGG	16	chr19	23347776	255	34M	*	0	0	*	*	XA:i:1	MD:Z:9T24	NM:i:1	UG:i:467	BX:Z:AAAGG
+SRR2057595.10043446_CAGAC	0	chr19	5493782	255	41M	*	0	0	*	*	XA:i:0	MD:Z:41	NM:i:0	UG:i:218	BX:Z:CAGAC
+SRR2057595.10043768_GGCGT	16	chr19	5797126	255	67M	*	0	0	*	*	XA:i:0	MD:Z:67	NM:i:0	UG:i:233	BX:Z:GGCGT
+SRR2057595.10043877_TGTTC	16	chr19	44470840	255	67M	*	0	0	*	*	XA:i:0	MD:Z:67	NM:i:0	UG:i:605	BX:Z:TGTTC
+SRR2057595.10043959_GACGT	0	chr19	40194275	255	30M	*	0	0	*	*	XA:i:0	MD:Z:30	NM:i:0	UG:i:565	BX:Z:GACGT
+SRR2057595.10044281_GCCGA	16	chr19	7389994	255	48M	*	0	0	*	*	XA:i:1	MD:Z:47C0	NM:i:1	UG:i:274	BX:Z:GCCGA
+SRR2057595.10044428_TCTAC	0	chr19	8798544	255	51M	*	0	0	*	*	XA:i:0	MD:Z:51	NM:i:0	UG:i:288	BX:Z:TCTAC
+SRR2057595.10044882_TTAAA	16	chr19	4785463	255	21M	*	0	0	*	*	XA:i:0	MD:Z:21	NM:i:0	UG:i:196	BX:Z:TTAAA
+SRR2057595.10044970_GTGGA	16	chr19	35902240	255	30M	*	0	0	*	*	XA:i:0	MD:Z:30	NM:i:0	UG:i:535	BX:Z:GTGGA
+SRR2057595.10045235_CGGCC	16	chr19	52641725	255	20M	*	0	0	*	*	XA:i:2	MD:Z:1C13A4	NM:i:2	UG:i:648	BX:Z:CGGCC
+SRR2057595.10045410_TGGTC	16	chr19	4078419	255	67M	*	0	0	*	*	XA:i:2	MD:Z:3T19A43	NM:i:2	UG:i:119	BX:Z:TGGTC
+SRR2057595.10045875_TTGGC	16	chr19	36085526	255	67M	*	0	0	*	*	XA:i:0	MD:Z:67	NM:i:0	UG:i:536	BX:Z:TTGGC
+SRR2057595.10045996_TAACA	16	chr19	4078298	255	50M	*	0	0	*	*	XA:i:2	MD:Z:42G0A6	NM:i:2	UG:i:32	BX:Z:TAACA
+SRR2057595.10046273_TCCAG	0	chr19	29662201	255	26M	*	0	0	*	*	XA:i:1	MD:Z:0C25	NM:i:1	UG:i:509	BX:Z:TCCAG
+SRR2057595.10047157_GTGGA	16	chr19	35902240	255	30M	*	0	0	*	*	XA:i:0	MD:Z:30	NM:i:0	UG:i:535	BX:Z:GTGGA
+SRR2057595.10047180_CCTCA	0	chr19	8798505	255	48M	*	0	0	*	*	XA:i:0	MD:Z:48	NM:i:0	UG:i:292	BX:Z:CCTCA
+SRR2057595.10047439_AATTA	16	chr19	50733890	255	61M	*	0	0	*	*	XA:i:2	MD:Z:55C0C4	NM:i:2	UG:i:645	BX:Z:AATTA
+SRR2057595.10047481_ACGCA	16	chr19	5796074	255	67M	*	0	0	*	*	XA:i:0	MD:Z:67	NM:i:0	UG:i:230	BX:Z:ACGCA
+SRR2057595.10047803_TTCGT	16	chr19	40658723	255	67M	*	0	0	*	*	XA:i:1	MD:Z:53T13	NM:i:1	UG:i:576	BX:Z:TTCGT
+SRR2057595.10047915_TCACA	0	chr19	13129273	255	45M	*	0	0	*	*	XA:i:2	MD:Z:25C1T17	NM:i:2	UG:i:388	BX:Z:TCACA
+SRR2057595.10048372_GTCCC	0	chr19	12347657	255	32M	*	0	0	*	*	XA:i:0	MD:Z:32	NM:i:0	UG:i:372	BX:Z:GTCCC
+SRR2057595.10048372_GTCCC	0	chr19	12348043	255	32M	*	0	0	*	*	XA:i:0	MD:Z:32	NM:i:0	UG:i:374	BX:Z:GTCCC
+SRR2057595.10048372_GTCCC	16	chr19	15035071	255	32M	*	0	0	*	*	XA:i:1	MD:Z:29T2	NM:i:1	UG:i:428	BX:Z:GTCCC
+SRR2057595.10048721_CTAAT	0	chr19	40194243	255	44M	*	0	0	*	*	XA:i:2	MD:Z:5T20A17	NM:i:2	UG:i:562	BX:Z:CTAAT
+SRR2057595.10048861_CTAGA	16	chr19	4078299	255	50M	*	0	0	*	*	XA:i:2	MD:Z:41G0A7	NM:i:2	UG:i:43	BX:Z:CTAGA
+SRR2057595.10048933_GTCGC	0	chr19	12080548	255	51M	*	0	0	*	*	XA:i:2	MD:Z:28C20G1	NM:i:2	UG:i:345	BX:Z:GTCGC
+SRR2057595.10048933_GTCGC	16	chr19	12084189	255	51M	*	0	0	*	*	XA:i:2	MD:Z:1C20G28	NM:i:2	UG:i:352	BX:Z:GTCGC
+SRR2057595.10049852_AAAGG	16	chr19	23347776	255	34M	*	0	0	*	*	XA:i:1	MD:Z:9T24	NM:i:1	UG:i:467	BX:Z:AAAGG
+SRR2057595.10049896_TTCGC	16	chr19	4078428	255	67M	*	0	0	*	*	XA:i:2	MD:Z:14A24T27	NM:i:2	UG:i:74	BX:Z:TTCGC
+SRR2057595.10050290_AAAGA	0	chr19	42921257	255	44M	*	0	0	*	*	XA:i:2	MD:Z:4G9G29	NM:i:2	UG:i:590	BX:Z:AAAGA
+SRR2057595.10050342_TGAAC	16	chr19	5798677	255	38M	*	0	0	*	*	XA:i:0	MD:Z:38	NM:i:0	UG:i:250	BX:Z:TGAAC
+SRR2057595.10050663_GACGT	0	chr19	40194275	255	30M	*	0	0	*	*	XA:i:0	MD:Z:30	NM:i:0	UG:i:565	BX:Z:GACGT
+SRR2057595.10051008_TTCGT	16	chr19	40658723	255	67M	*	0	0	*	*	XA:i:1	MD:Z:53T13	NM:i:1	UG:i:576	BX:Z:TTCGT
+SRR2057595.10051043_GCCGA	16	chr19	4078298	255	64M	*	0	0	*	*	XA:i:1	MD:Z:43A20	NM:i:1	UG:i:66	BX:Z:GCCGA
+SRR2057595.1005223_TTCGC	16	chr19	4078428	255	67M	*	0	0	*	*	XA:i:2	MD:Z:14A24T27	NM:i:2	UG:i:74	BX:Z:TTCGC
+SRR2057595.10052510_ATACG	0	chr19	13129421	255	31M	*	0	0	*	*	XA:i:1	MD:Z:11A19	NM:i:1	UG:i:393	BX:Z:ATACG
+SRR2057595.10052666_ATCCG	16	chr19	37369602	255	35M	*	0	0	*	*	XA:i:0	MD:Z:35	NM:i:0	UG:i:544	BX:Z:ATCCG
+SRR2057595.10053188_CTTTC	16	chr19	4078298	255	45M	*	0	0	*	*	XA:i:1	MD:Z:43A1	NM:i:1	UG:i:15	BX:Z:CTTTC
+SRR2057595.10053341_TTGCA	0	chr19	41640185	255	61M	*	0	0	*	*	XA:i:0	MD:Z:61	NM:i:0	UG:i:581	BX:Z:TTGCA
+SRR2057595.10053375_ATGAA	0	chr19	56007800	255	67M	*	0	0	*	*	XA:i:0	MD:Z:67	NM:i:0	UG:i:666	BX:Z:ATGAA
+SRR2057595.10053481_CTCCG	0	chr19	11195966	255	44M	*	0	0	*	*	XA:i:1	MD:Z:0T43	NM:i:1	UG:i:338	BX:Z:CTCCG
+SRR2057595.10053518_AATCG	16	chr19	4078303	255	67M	*	0	0	*	*	XA:i:2	MD:Z:38A21A6	NM:i:2	UG:i:88	BX:Z:AATCG
+SRR2057595.1005354_GAGAG	16	chr19	4078298	255	50M	*	0	0	*	*	XA:i:1	MD:Z:43A6	NM:i:1	UG:i:35	BX:Z:GAGAG
+SRR2057595.1005379_CCAAC	0	chr19	8820047	255	33M	*	0	0	*	*	XA:i:0	MD:Z:33	NM:i:0	UG:i:315	BX:Z:CCAAC
+SRR2057595.10053918_AGTAT	0	chr19	10600420	255	29M	*	0	0	*	*	XA:i:0	MD:Z:29	NM:i:0	UG:i:328	BX:Z:AGTAT
+SRR2057595.10053970_TATGG	0	chr19	13129322	255	56M	*	0	0	*	*	XA:i:2	MD:Z:33C5A16	NM:i:2	UG:i:405	BX:Z:TATGG
+SRR2057595.10054046_ATAAA	16	chr19	4078298	255	37M	*	0	0	*	*	XA:i:0	MD:Z:37	NM:i:0	UG:i:175	BX:Z:ATAAA
+SRR2057595.10054497_AAAGA	0	chr19	42921257	255	44M	*	0	0	*	*	XA:i:2	MD:Z:4G9G29	NM:i:2	UG:i:590	BX:Z:AAAGA
+SRR2057595.10055409_TAGAC	0	chr19	23806079	255	67M	*	0	0	*	*	XA:i:1	MD:Z:41T25	NM:i:1	UG:i:468	BX:Z:TAGAC
+SRR2057595.10055838_CAGAC	0	chr19	5493782	255	41M	*	0	0	*	*	XA:i:0	MD:Z:41	NM:i:0	UG:i:218	BX:Z:CAGAC
+SRR2057595.10055912_TCTCA	16	chr19	4078298	255	50M	*	0	0	*	*	XA:i:1	MD:Z:43A6	NM:i:1	UG:i:36	BX:Z:TCTCA
+SRR2057595.10055955_AGGAT	16	chr19	4078296	255	67M	*	0	0	*	*	XA:i:2	MD:Z:0T44A21	NM:i:2	UG:i:70	BX:Z:AGGAT
+SRR2057595.10056203_TGCAA	16	chr19	61240267	255	28M	*	0	0	*	*	XA:i:1	MD:Z:11C16	NM:i:1	UG:i:703	BX:Z:TGCAA
+SRR2057595.10056249_TCAGG	0	chr19	13129322	255	35M	*	0	0	*	*	XA:i:1	MD:Z:33C1	NM:i:1	UG:i:407	BX:Z:TCAGG
+SRR2057595.10057756_CGAAC	0	chr19	13129273	255	36M	*	0	0	*	*	XA:i:2	MD:Z:25C1T8	NM:i:2	UG:i:386	BX:Z:CGAAC
+SRR2057595.10058241_TTAAA	16	chr19	4785463	255	21M	*	0	0	*	*	XA:i:0	MD:Z:21	NM:i:0	UG:i:196	BX:Z:TTAAA
+SRR2057595.10058572_TCTTA	16	chr19	61240267	255	28M	*	0	0	*	*	XA:i:1	MD:Z:11C16	NM:i:1	UG:i:705	BX:Z:TCTTA
+SRR2057595.10058802_TAGTA	0	chr19	10274608	255	34M	*	0	0	*	*	XA:i:1	MD:Z:9C24	NM:i:1	UG:i:324	BX:Z:TAGTA
+SRR2057595.10058946_AGCCA	0	chr19	43786861	255	67M	*	0	0	*	*	XA:i:0	MD:Z:67	NM:i:0	UG:i:594	BX:Z:AGCCA
+SRR2057595.10059254_GGTTC	16	chr19	56896659	255	23M	*	0	0	*	*	XA:i:2	MD:Z:0C0A21	NM:i:2	UG:i:674	BX:Z:GGTTC
+SRR2057595.10059642_CTAGC	16	chr19	4078298	255	62M	*	0	0	*	*	XA:i:1	MD:Z:43A18	NM:i:1	UG:i:63	BX:Z:CTAGC
+SRR2057595.10060193_ATAGA	0	chr19	38795805	255	62M	*	0	0	*	*	XA:i:2	MD:Z:44C1A15	NM:i:2	UG:i:553	BX:Z:ATAGA
+SRR2057595.10060270_ATGGT	16	chr19	12086074	255	45M	*	0	0	*	*	XA:i:1	MD:Z:19T25	NM:i:1	UG:i:361	BX:Z:ATGGT
+SRR2057595.10060437_TATAT	0	chr19	40194298	255	25M	*	0	0	*	*	XA:i:1	MD:Z:13A11	NM:i:1	UG:i:567	BX:Z:TATAT
+SRR2057595.10060447_CTTAC	16	chr19	4078298	255	54M	*	0	0	*	*	XA:i:2	MD:Z:42G0A10	NM:i:2	UG:i:49	BX:Z:CTTAC
+SRR2057595.10060568_GACGC	16	chr19	32419974	255	67M	*	0	0	*	*	XA:i:0	MD:Z:67	NM:i:0	UG:i:514	BX:Z:GACGC
+SRR2057595.10060757_AGCCG	16	chr19	4078299	255	38M	*	0	0	*	*	XA:i:0	MD:Z:38	NM:i:0	UG:i:11	BX:Z:AGCCG
+SRR2057595.10060776_TCTTG	16	chr19	4078423	255	67M	*	0	0	*	*	XA:i:1	MD:Z:19A47	NM:i:1	UG:i:111	BX:Z:TCTTG
+SRR2057595.10061005_AGCCG	16	chr19	4078299	255	36M	*	0	0	*	*	XA:i:0	MD:Z:36	NM:i:0	UG:i:173	BX:Z:AGCCG
+SRR2057595.10061018_CACTG	16	chr19	5798673	255	42M	*	0	0	*	*	XA:i:0	MD:Z:42	NM:i:0	UG:i:249	BX:Z:CACTG
+SRR2057595.10061192_ATCCG	16	chr19	37369602	255	35M	*	0	0	*	*	XA:i:0	MD:Z:35	NM:i:0	UG:i:544	BX:Z:ATCCG
+SRR2057595.10061371_GCCTG	16	chr19	4078301	255	67M	*	0	0	*	*	XA:i:2	MD:Z:40A21A4	NM:i:2	UG:i:75	BX:Z:GCCTG
+SRR2057595.10061403_AAGTA	16	chr19	31326011	255	57M	*	0	0	*	*	XA:i:1	MD:Z:23A33	NM:i:1	UG:i:512	BX:Z:AAGTA
+SRR2057595.10061424_GCTAC	16	chr19	4078446	255	67M	*	0	0	*	*	XA:i:0	MD:Z:67	NM:i:0	UG:i:97	BX:Z:GCTAC
+SRR2057595.10061535_ATCAA	0	chr19	20547357	255	23M	*	0	0	*	*	XA:i:0	MD:Z:23	NM:i:0	UG:i:452	BX:Z:ATCAA
+SRR2057595.10061661_ACAAA	16	chr19	5688438	255	28M	*	0	0	*	*	XA:i:0	MD:Z:28	NM:i:0	UG:i:222	BX:Z:ACAAA
+SRR2057595.10061745_ATATA	0	chr19	13129330	255	22M	*	0	0	*	*	XA:i:1	MD:Z:0T21	NM:i:1	UG:i:422	BX:Z:ATATA
+SRR2057595.10062673_ATACC	16	chr19	35673152	255	21M	*	0	0	*	*	XA:i:2	MD:Z:7T2A10	NM:i:2	UG:i:534	BX:Z:ATACC
+SRR2057595.10062948_TTTTC	16	chr19	4078296	255	67M	*	0	0	*	*	XA:i:2	MD:Z:0T44A21	NM:i:2	UG:i:69	BX:Z:TTTTC
+SRR2057595.10063079_GTCTA	16	chr19	4078420	255	67M	*	0	0	*	*	XA:i:2	MD:Z:2T19A44	NM:i:2	UG:i:124	BX:Z:GTCTA
+SRR2057595.10063521_AGATG	16	chr19	61240273	255	22M	*	0	0	*	*	XA:i:1	MD:Z:5C16	NM:i:1	UG:i:707	BX:Z:AGATG
+SRR2057595.10064036_CAGCA	16	chr19	5801892	255	47M	*	0	0	*	*	XA:i:0	MD:Z:47	NM:i:0	UG:i:258	BX:Z:CAGCA
+SRR2057595.1006417_GATCA	16	chr19	4078298	255	50M	*	0	0	*	*	XA:i:2	MD:Z:42G0A6	NM:i:2	UG:i:34	BX:Z:GATCA
+SRR2057595.10065256_AGGGG	0	chr19	13129331	255	37M	*	0	0	*	*	XA:i:2	MD:Z:24C5A6	NM:i:2	UG:i:423	BX:Z:AGGGG
+SRR2057595.10065460_ATGGT	0	chr19	8798504	255	50M	*	0	0	*	*	XA:i:0	MD:Z:50	NM:i:0	UG:i:291	BX:Z:ATGGT
+SRR2057595.10065656_GATGA	0	chr19	48194137	255	59M	*	0	0	*	*	XA:i:0	MD:Z:59	NM:i:0	UG:i:635	BX:Z:GATGA
+SRR2057595.10065671_ATGAT	16	chr19	61240306	255	36M	*	0	0	*	*	XA:i:2	MD:Z:16A4T14	NM:i:2	UG:i:716	BX:Z:ATGAT
+SRR2057595.10065713_ATGTC	0	chr19	37052811	255	64M	*	0	0	*	*	XA:i:1	MD:Z:11C52	NM:i:1	UG:i:542	BX:Z:ATGTC
+SRR2057595.10065896_GCTAT	0	chr19	57701750	255	67M	*	0	0	*	*	XA:i:2	MD:Z:34T1T30	NM:i:2	UG:i:677	BX:Z:GCTAT
+SRR2057595.10066051_AACTT	16	chr19	4078420	255	64M	*	0	0	*	*	XA:i:2	MD:Z:2T19A41	NM:i:2	UG:i:55	BX:Z:AACTT
+SRR2057595.10066334_AGCCA	0	chr19	47324044	255	46M	*	0	0	*	*	XA:i:0	MD:Z:46	NM:i:0	UG:i:627	BX:Z:AGCCA
+SRR2057595.10066646_GGTTC	16	chr19	56896659	255	23M	*	0	0	*	*	XA:i:2	MD:Z:0C0A21	NM:i:2	UG:i:674	BX:Z:GGTTC
+SRR2057595.10066775_GTCAA	16	chr19	4078298	255	57M	*	0	0	*	*	XA:i:1	MD:Z:43A13	NM:i:1	UG:i:52	BX:Z:GTCAA
+SRR2057595.10066849_GGTCG	0	chr19	53686815	255	67M	*	0	0	*	*	XA:i:0	MD:Z:67	NM:i:0	UG:i:651	BX:Z:GGTCG
+SRR2057595.10066970_TAACA	16	chr19	4078298	255	50M	*	0	0	*	*	XA:i:2	MD:Z:42G0A6	NM:i:2	UG:i:32	BX:Z:TAACA
+SRR2057595.10067118_TGATT	16	chr19	4078299	255	50M	*	0	0	*	*	XA:i:2	MD:Z:41G0A7	NM:i:2	UG:i:41	BX:Z:TGATT
+SRR2057595.10067277_TAGAC	0	chr19	23806079	255	67M	*	0	0	*	*	XA:i:1	MD:Z:41T25	NM:i:1	UG:i:468	BX:Z:TAGAC
+SRR2057595.10067458_TACGG	16	chr19	4078296	255	67M	*	0	0	*	*	XA:i:2	MD:Z:1T43A21	NM:i:2	UG:i:71	BX:Z:TACGG
+SRR2057595.10067669_GCTTG	16	chr19	5797471	255	50M	*	0	0	*	*	XA:i:0	MD:Z:50	NM:i:0	UG:i:226	BX:Z:GCTTG
+SRR2057595.10067834_GACGT	0	chr19	40194275	255	30M	*	0	0	*	*	XA:i:0	MD:Z:30	NM:i:0	UG:i:565	BX:Z:GACGT
+SRR2057595.1006784_GTCCA	16	chr19	4078431	255	67M	*	0	0	*	*	XA:i:1	MD:Z:11A55	NM:i:1	UG:i:107	BX:Z:GTCCA
+SRR2057595.10067875_GCCGA	16	chr19	4078298	255	64M	*	0	0	*	*	XA:i:1	MD:Z:43A20	NM:i:1	UG:i:66	BX:Z:GCCGA
+SRR2057595.1006816_GATCA	16	chr19	4078298	255	50M	*	0	0	*	*	XA:i:2	MD:Z:42G0A6	NM:i:2	UG:i:34	BX:Z:GATCA
+SRR2057595.10068282_TAACA	0	chr19	8799023	255	67M	*	0	0	*	*	XA:i:0	MD:Z:67	NM:i:0	UG:i:299	BX:Z:TAACA
+SRR2057595.1006840_TTAAA	16	chr19	4785463	255	21M	*	0	0	*	*	XA:i:0	MD:Z:21	NM:i:0	UG:i:196	BX:Z:TTAAA
+SRR2057595.10068743_TTGGC	16	chr19	36085526	255	67M	*	0	0	*	*	XA:i:0	MD:Z:67	NM:i:0	UG:i:536	BX:Z:TTGGC
+SRR2057595.10068873_CTTTT	0	chr19	56656380	255	54M	*	0	0	*	*	XA:i:0	MD:Z:54	NM:i:0	UG:i:669	BX:Z:CTTTT
+SRR2057595.10068999_AAGTA	16	chr19	31326011	255	57M	*	0	0	*	*	XA:i:1	MD:Z:23A33	NM:i:1	UG:i:512	BX:Z:AAGTA
+SRR2057595.10069428_AACGC	16	chr19	4078298	255	51M	*	0	0	*	*	XA:i:1	MD:Z:43A7	NM:i:1	UG:i:42	BX:Z:AACGC
+SRR2057595.10070094_TCTTA	16	chr19	61240267	255	28M	*	0	0	*	*	XA:i:1	MD:Z:11C16	NM:i:1	UG:i:705	BX:Z:TCTTA
+SRR2057595.10070448_GGATC	16	chr19	4078298	255	61M	*	0	0	*	*	XA:i:2	MD:Z:42G0A17	NM:i:2	UG:i:61	BX:Z:GGATC
+SRR2057595.1007050_TATAC	0	chr19	8799026	255	60M	*	0	0	*	*	XA:i:0	MD:Z:60	NM:i:0	UG:i:301	BX:Z:TATAC
+SRR2057595.10070871_GAAGT	16	chr19	27988575	255	52M	*	0	0	*	*	XA:i:0	MD:Z:52	NM:i:0	UG:i:494	BX:Z:GAAGT
+SRR2057595.10071095_TGCAA	16	chr19	61240267	255	28M	*	0	0	*	*	XA:i:1	MD:Z:11C16	NM:i:1	UG:i:703	BX:Z:TGCAA
+SRR2057595.10071228_CTAGC	16	chr19	4078298	255	62M	*	0	0	*	*	XA:i:1	MD:Z:43A18	NM:i:1	UG:i:63	BX:Z:CTAGC
+SRR2057595.10071424_CATTT	0	chr19	49059158	255	49M	*	0	0	*	*	XA:i:2	MD:Z:30A7A10	NM:i:2	UG:i:639	BX:Z:CATTT
+SRR2057595.10071487_CACTG	16	chr19	5798673	255	42M	*	0	0	*	*	XA:i:0	MD:Z:42	NM:i:0	UG:i:249	BX:Z:CACTG
+SRR2057595.10072063_CAGCA	16	chr19	5801892	255	47M	*	0	0	*	*	XA:i:0	MD:Z:47	NM:i:0	UG:i:258	BX:Z:CAGCA
+SRR2057595.10072068_GCCGA	16	chr19	4078298	255	64M	*	0	0	*	*	XA:i:1	MD:Z:43A20	NM:i:1	UG:i:66	BX:Z:GCCGA
+SRR2057595.10072172_TCTGC	16	chr19	4078299	255	36M	*	0	0	*	*	XA:i:0	MD:Z:36	NM:i:0	UG:i:178	BX:Z:TCTGC
+SRR2057595.10072478_AGCTT	16	chr19	5799262	255	20M	*	0	0	*	*	XA:i:0	MD:Z:20	NM:i:0	UG:i:245	BX:Z:AGCTT
+SRR2057595.10072601_TTGGC	16	chr19	4078302	255	67M	*	0	0	*	*	XA:i:2	MD:Z:39A21A5	NM:i:2	UG:i:79	BX:Z:TTGGC
+SRR2057595.10072627_AATAC	0	chr19	12046308	255	33M	*	0	0	*	*	XA:i:1	MD:Z:23A9	NM:i:1	UG:i:343	BX:Z:AATAC
+SRR2057595.10072909_TCCAG	0	chr19	29662201	255	26M	*	0	0	*	*	XA:i:1	MD:Z:0C25	NM:i:1	UG:i:509	BX:Z:TCCAG
+SRR2057595.10072922_TGATT	16	chr19	4078299	255	50M	*	0	0	*	*	XA:i:2	MD:Z:41G0A7	NM:i:2	UG:i:41	BX:Z:TGATT
+SRR2057595.10072936_TAGTA	16	chr19	61240268	255	27M	*	0	0	*	*	XA:i:1	MD:Z:10C16	NM:i:1	UG:i:702	BX:Z:TAGTA
+SRR2057595.10073020_TGTTT	0	chr19	41595518	255	38M	*	0	0	*	*	XA:i:1	MD:Z:25G12	NM:i:1	UG:i:579	BX:Z:TGTTT
+SRR2057595.10073067_CGTCT	0	chr19	13129329	255	35M	*	0	0	*	*	XA:i:2	MD:Z:26C5A2	NM:i:2	UG:i:421	BX:Z:CGTCT
+SRR2057595.10073092_TTTTC	16	chr19	4078296	255	67M	*	0	0	*	*	XA:i:2	MD:Z:0T44A21	NM:i:2	UG:i:69	BX:Z:TTTTC
+SRR2057595.1007313_ACATT	0	chr19	8799370	255	64M	*	0	0	*	*	XA:i:0	MD:Z:64	NM:i:0	UG:i:297	BX:Z:ACATT
+SRR2057595.10073348_CCTTG	16	chr19	4078298	255	49M	*	0	0	*	*	XA:i:1	MD:Z:43A5	NM:i:1	UG:i:24	BX:Z:CCTTG
+SRR2057595.10073515_GGATC	16	chr19	4078298	255	61M	*	0	0	*	*	XA:i:2	MD:Z:42G0A17	NM:i:2	UG:i:61	BX:Z:GGATC
+SRR2057595.10073784_TGATG	16	chr19	3544146	255	39M	*	0	0	*	*	XA:i:0	MD:Z:39	NM:i:0	UG:i:1	BX:Z:TGATG
+SRR2057595.10073896_TTCGT	0	chr19	36922761	255	57M	*	0	0	*	*	XA:i:1	MD:Z:5C51	NM:i:1	UG:i:538	BX:Z:TTCGT
+SRR2057595.10073986_ATCTC	0	chr19	21910875	255	27M	*	0	0	*	*	XA:i:0	MD:Z:27	NM:i:0	UG:i:458	BX:Z:ATCTC
+SRR2057595.10074218_AGTGC	0	chr19	8798525	255	27M	*	0	0	*	*	XA:i:0	MD:Z:27	NM:i:0	UG:i:296	BX:Z:AGTGC
+SRR2057595.10074241_AAACA	16	chr19	4078299	255	49M	*	0	0	*	*	XA:i:1	MD:Z:42A6	NM:i:1	UG:i:33	BX:Z:AAACA
+SRR2057595.10074267_GCTAC	0	chr19	13129281	255	31M	*	0	0	*	*	XA:i:2	MD:Z:17C1T11	NM:i:2	UG:i:391	BX:Z:GCTAC
+SRR2057595.10074447_ATTAT	16	chr19	7546213	255	27M	*	0	0	*	*	XA:i:0	MD:Z:27	NM:i:0	UG:i:278	BX:Z:ATTAT
+SRR2057595.10074845_GTTGT	16	chr19	12564367	255	21M	*	0	0	*	*	XA:i:2	MD:Z:0A12A7	NM:i:2	UG:i:380	BX:Z:GTTGT
+SRR2057595.10075349_ATGAT	16	chr19	61240306	255	36M	*	0	0	*	*	XA:i:2	MD:Z:16A4T14	NM:i:2	UG:i:716	BX:Z:ATGAT
+SRR2057595.10075433_GGGGC	16	chr19	4078446	255	67M	*	0	0	*	*	XA:i:1	MD:Z:66T0	NM:i:1	UG:i:98	BX:Z:GGGGC
+SRR2057595.10075536_GCATA	16	chr19	4078298	255	57M	*	0	0	*	*	XA:i:2	MD:Z:42G0A13	NM:i:2	UG:i:53	BX:Z:GCATA
+SRR2057595.10075745_GTATT	0	chr19	47323072	255	47M	*	0	0	*	*	XA:i:1	MD:Z:0C46	NM:i:1	UG:i:628	BX:Z:GTATT
+SRR2057595.10075769_ATTAT	16	chr19	7546213	255	27M	*	0	0	*	*	XA:i:0	MD:Z:27	NM:i:0	UG:i:278	BX:Z:ATTAT
+SRR2057595.10075776_GCTAC	16	chr19	4078446	255	67M	*	0	0	*	*	XA:i:0	MD:Z:67	NM:i:0	UG:i:97	BX:Z:GCTAC
+SRR2057595.10075922_AATAC	0	chr19	12046308	255	33M	*	0	0	*	*	XA:i:1	MD:Z:23A9	NM:i:1	UG:i:343	BX:Z:AATAC
+SRR2057595.10075965_GCATA	16	chr19	4078298	255	57M	*	0	0	*	*	XA:i:2	MD:Z:42G0A13	NM:i:2	UG:i:53	BX:Z:GCATA
+SRR2057595.10076063_AAATC	16	chr19	47891298	255	21M	*	0	0	*	*	XA:i:2	MD:Z:4C11C4	NM:i:2	UG:i:633	BX:Z:AAATC
+SRR2057595.10076706_TTGCA	0	chr19	44434953	255	43M	*	0	0	*	*	XA:i:0	MD:Z:43	NM:i:0	UG:i:602	BX:Z:TTGCA
+SRR2057595.1007711_TCCAG	0	chr19	29662201	255	26M	*	0	0	*	*	XA:i:1	MD:Z:0C25	NM:i:1	UG:i:509	BX:Z:TCCAG
+SRR2057595.10077136_ATGTC	0	chr19	37052811	255	64M	*	0	0	*	*	XA:i:1	MD:Z:11C52	NM:i:1	UG:i:542	BX:Z:ATGTC
+SRR2057595.10077390_GATCA	16	chr19	4078298	255	50M	*	0	0	*	*	XA:i:2	MD:Z:42G0A6	NM:i:2	UG:i:34	BX:Z:GATCA
+SRR2057595.10077550_GATCA	16	chr19	4078298	255	50M	*	0	0	*	*	XA:i:2	MD:Z:42G0A6	NM:i:2	UG:i:34	BX:Z:GATCA
+SRR2057595.10078118_AGCGC	16	chr19	27464368	255	52M	*	0	0	*	*	XA:i:0	MD:Z:52	NM:i:0	UG:i:493	BX:Z:AGCGC
+SRR2057595.10078219_GCATA	16	chr19	4078298	255	57M	*	0	0	*	*	XA:i:2	MD:Z:42G0A13	NM:i:2	UG:i:53	BX:Z:GCATA
+SRR2057595.10078231_ATGAT	16	chr19	61240306	255	36M	*	0	0	*	*	XA:i:2	MD:Z:16A4T14	NM:i:2	UG:i:716	BX:Z:ATGAT
+SRR2057595.10078601_AATGC	0	chr19	8798773	255	42M	*	0	0	*	*	XA:i:0	MD:Z:42	NM:i:0	UG:i:306	BX:Z:AATGC
+SRR2057595.10079068_TTGGC	16	chr19	4078302	255	67M	*	0	0	*	*	XA:i:2	MD:Z:39A21A5	NM:i:2	UG:i:79	BX:Z:TTGGC
+SRR2057595.10079368_GAGTG	0	chr19	8798502	255	51M	*	0	0	*	*	XA:i:0	MD:Z:51	NM:i:0	UG:i:289	BX:Z:GAGTG
+SRR2057595.10079546_CGGCC	16	chr19	52641725	255	20M	*	0	0	*	*	XA:i:2	MD:Z:1C13A4	NM:i:2	UG:i:648	BX:Z:CGGCC
+SRR2057595.10079549_TCTTA	16	chr19	61240267	255	28M	*	0	0	*	*	XA:i:1	MD:Z:11C16	NM:i:1	UG:i:705	BX:Z:TCTTA
+SRR2057595.10079658_CGAAC	0	chr19	13129273	255	36M	*	0	0	*	*	XA:i:2	MD:Z:25C1T8	NM:i:2	UG:i:386	BX:Z:CGAAC
+SRR2057595.10080019_TATGG	0	chr19	13129322	255	56M	*	0	0	*	*	XA:i:2	MD:Z:33C5A16	NM:i:2	UG:i:405	BX:Z:TATGG
+SRR2057595.10080347_GCAGA	0	chr19	8799844	255	53M	*	0	0	*	*	XA:i:0	MD:Z:53	NM:i:0	UG:i:309	BX:Z:GCAGA
+SRR2057595.10080699_TTTTC	16	chr19	4078296	255	67M	*	0	0	*	*	XA:i:2	MD:Z:0T44A21	NM:i:2	UG:i:69	BX:Z:TTTTC
+SRR2057595.10080920_GCTAT	16	chr19	61240267	255	28M	*	0	0	*	*	XA:i:1	MD:Z:11C16	NM:i:1	UG:i:706	BX:Z:GCTAT
+SRR2057595.10080946_TATAT	0	chr19	40194298	255	25M	*	0	0	*	*	XA:i:1	MD:Z:13A11	NM:i:1	UG:i:567	BX:Z:TATAT
+SRR2057595.10081070_CGCCG	16	chr19	4078299	255	59M	*	0	0	*	*	XA:i:1	MD:Z:42A16	NM:i:1	UG:i:58	BX:Z:CGCCG
+SRR2057595.10081274_AACGC	16	chr19	4078298	255	51M	*	0	0	*	*	XA:i:1	MD:Z:43A7	NM:i:1	UG:i:42	BX:Z:AACGC
+SRR2057595.10081914_AATAC	16	chr19	4078420	255	67M	*	0	0	*	*	XA:i:2	MD:Z:2T19A44	NM:i:2	UG:i:125	BX:Z:AATAC
+SRR2057595.10082001_TCTTG	16	chr19	61240267	255	28M	*	0	0	*	*	XA:i:1	MD:Z:11C16	NM:i:1	UG:i:705	BX:Z:TCTTA
+SRR2057595.1008200_TTCGC	16	chr19	4078428	255	67M	*	0	0	*	*	XA:i:2	MD:Z:14A24T27	NM:i:2	UG:i:74	BX:Z:TTCGC
+SRR2057595.1008230_GCTAT	0	chr19	57701750	255	67M	*	0	0	*	*	XA:i:2	MD:Z:34T1T30	NM:i:2	UG:i:677	BX:Z:GCTAT
+SRR2057595.10082591_ACAGA	16	chr19	4078299	255	22M	*	0	0	*	*	XA:i:2	MD:Z:0C0C20	NM:i:2	UG:i:136	BX:Z:ACAGA
+SRR2057595.10082733_CCTTT	16	chr19	4078298	255	37M	*	0	0	*	*	XA:i:0	MD:Z:37	NM:i:0	UG:i:180	BX:Z:CCTTT
+SRR2057595.1008274_ATCAA	0	chr19	20547357	255	23M	*	0	0	*	*	XA:i:0	MD:Z:23	NM:i:0	UG:i:452	BX:Z:ATCAA
+SRR2057595.100827_ACAGA	16	chr19	4078299	255	22M	*	0	0	*	*	XA:i:2	MD:Z:0C0C20	NM:i:2	UG:i:136	BX:Z:ACAGA
+SRR2057595.10082802_GCCGT	0	chr19	30861987	255	20M	*	0	0	*	*	XA:i:2	MD:Z:3C2T13	NM:i:2	UG:i:511	BX:Z:GCCGT
+SRR2057595.10082815_CCTCA	0	chr19	8798505	255	48M	*	0	0	*	*	XA:i:0	MD:Z:48	NM:i:0	UG:i:292	BX:Z:CCTCA
+SRR2057595.1008318_CTATT	16	chr19	56874049	255	67M	*	0	0	*	*	XA:i:0	MD:Z:67	NM:i:0	UG:i:672	BX:Z:CTATT
+SRR2057595.10084177_ATCAA	0	chr19	20547357	255	23M	*	0	0	*	*	XA:i:0	MD:Z:23	NM:i:0	UG:i:452	BX:Z:ATCAA
+SRR2057595.1008424_GATAA	16	chr19	47057431	255	22M	*	0	0	*	*	XA:i:2	MD:Z:6T10T4	NM:i:2	UG:i:622	BX:Z:GATAA
+SRR2057595.10084297_CTTCA	0	chr19	13129483	255	30M	*	0	0	*	*	XA:i:2	MD:Z:6G16A6	NM:i:2	UG:i:382	BX:Z:CTTCA
+SRR2057595.10084300_AGCCG	16	chr19	4078299	255	36M	*	0	0	*	*	XA:i:0	MD:Z:36	NM:i:0	UG:i:173	BX:Z:AGCCG
+SRR2057595.10084403_GACGT	0	chr19	40194275	255	30M	*	0	0	*	*	XA:i:0	MD:Z:30	NM:i:0	UG:i:565	BX:Z:GACGT
+SRR2057595.10084641_ACGCA	16	chr19	51217013	255	67M	*	0	0	*	*	XA:i:2	MD:Z:3A46A16	NM:i:2	UG:i:647	BX:Z:ACGCA
+SRR2057595.10084680_CCCCG	16	chr19	4078298	255	38M	*	0	0	*	*	XA:i:0	MD:Z:38	NM:i:0	UG:i:184	BX:Z:CCCCG
+SRR2057595.1008532_CCGCG	0	chr19	8991126	255	28M	*	0	0	*	*	XA:i:0	MD:Z:28	NM:i:0	UG:i:318	BX:Z:CCGCG
+SRR2057595.1008533_AAGGT	0	chr19	8798514	255	39M	*	0	0	*	*	XA:i:0	MD:Z:39	NM:i:0	UG:i:294	BX:Z:AAGGT
+SRR2057595.10085421_CGAGA	0	chr19	6375345	255	21M	*	0	0	*	*	XA:i:2	MD:Z:10C5G4	NM:i:2	UG:i:265	BX:Z:CGAGA
+SRR2057595.10085567_TATTC	16	chr19	5038295	255	24M	*	0	0	*	*	XA:i:0	MD:Z:24	NM:i:0	UG:i:206	BX:Z:TATTC
+SRR2057595.10085614_AATTA	16	chr19	50733889	255	62M	*	0	0	*	*	XA:i:2	MD:Z:56C0C4	NM:i:2	UG:i:645	BX:Z:AATTA
+SRR2057595.10085673_GAAGT	16	chr19	8360277	255	67M	*	0	0	*	*	XA:i:2	MD:Z:10A52A3	NM:i:2	UG:i:285	BX:Z:GAAGT
+SRR2057595.10086547_TTCGT	0	chr19	47167097	255	67M	*	0	0	*	*	XA:i:0	MD:Z:67	NM:i:0	UG:i:623	BX:Z:TTCGT
+SRR2057595.10086746_TCTTC	16	chr19	4078299	255	36M	*	0	0	*	*	XA:i:1	MD:Z:27A8	NM:i:1	UG:i:178	BX:Z:TCTGC
+SRR2057595.1008697_TTGCA	0	chr19	44434953	255	43M	*	0	0	*	*	XA:i:0	MD:Z:43	NM:i:0	UG:i:602	BX:Z:TTGCA
+SRR2057595.10087261_TTTTC	16	chr19	4078296	255	67M	*	0	0	*	*	XA:i:2	MD:Z:0T44A21	NM:i:2	UG:i:69	BX:Z:TTTTC
+SRR2057595.10087496_CACTG	16	chr19	5798673	255	42M	*	0	0	*	*	XA:i:0	MD:Z:42	NM:i:0	UG:i:249	BX:Z:CACTG
+SRR2057595.10087607_ATACG	0	chr19	13129421	255	31M	*	0	0	*	*	XA:i:1	MD:Z:11A19	NM:i:1	UG:i:393	BX:Z:ATACG
+SRR2057595.10087628_GGCGT	16	chr19	5797126	255	67M	*	0	0	*	*	XA:i:0	MD:Z:67	NM:i:0	UG:i:233	BX:Z:GGCGT
+SRR2057595.10087735_TCTGC	16	chr19	4078299	255	36M	*	0	0	*	*	XA:i:0	MD:Z:36	NM:i:0	UG:i:178	BX:Z:TCTGC
+SRR2057595.10088373_AAAGG	16	chr19	23347776	255	34M	*	0	0	*	*	XA:i:1	MD:Z:9T24	NM:i:1	UG:i:467	BX:Z:AAAGG
+SRR2057595.1008837_GCGGA	16	chr19	4078298	255	48M	*	0	0	*	*	XA:i:1	MD:Z:43A4	NM:i:1	UG:i:19	BX:Z:GCGGA
+SRR2057595.10088477_AGTCG	0	chr19	17767763	255	67M	*	0	0	*	*	XA:i:2	MD:Z:10A4A51	NM:i:2	UG:i:440	BX:Z:AGTCG
+SRR2057595.1008854_TTCGT	0	chr19	47167097	255	67M	*	0	0	*	*	XA:i:0	MD:Z:67	NM:i:0	UG:i:623	BX:Z:TTCGT
+SRR2057595.10088899_CATAA	0	chr19	13129322	255	35M	*	0	0	*	*	XA:i:1	MD:Z:33C1	NM:i:1	UG:i:401	BX:Z:CATAA
+SRR2057595.10088953_ATACC	16	chr19	35673152	255	21M	*	0	0	*	*	XA:i:2	MD:Z:7T2A10	NM:i:2	UG:i:534	BX:Z:ATACC
+SRR2057595.10088992_TATTC	16	chr19	5038295	255	24M	*	0	0	*	*	XA:i:0	MD:Z:24	NM:i:0	UG:i:206	BX:Z:TATTC
+SRR2057595.10089226_AAACA	16	chr19	4078299	255	49M	*	0	0	*	*	XA:i:1	MD:Z:42A6	NM:i:1	UG:i:33	BX:Z:AAACA
+SRR2057595.10089360_ACCTG	16	chr19	4078420	255	67M	*	0	0	*	*	XA:i:2	MD:Z:2T19A44	NM:i:2	UG:i:126	BX:Z:ACCTG
+SRR2057595.10089372_CAAAC	0	chr19	34304620	255	34M	*	0	0	*	*	XA:i:0	MD:Z:34	NM:i:0	UG:i:531	BX:Z:CAAAC
+SRR2057595.10089645_CATTT	0	chr19	49059158	255	49M	*	0	0	*	*	XA:i:2	MD:Z:30A7A10	NM:i:2	UG:i:639	BX:Z:CATTT
+SRR2057595.10089847_GCCGA	16	chr19	7389994	255	48M	*	0	0	*	*	XA:i:1	MD:Z:47C0	NM:i:1	UG:i:274	BX:Z:GCCGA
+SRR2057595.10090471_ATTCT	0	chr19	3576314	255	23M	*	0	0	*	*	XA:i:2	MD:Z:16T5T0	NM:i:2	UG:i:5	BX:Z:ATTCT
+SRR2057595.10090859_TTGCA	0	chr19	44434953	255	43M	*	0	0	*	*	XA:i:0	MD:Z:43	NM:i:0	UG:i:602	BX:Z:TTGCA
+SRR2057595.10090930_CCTCG	0	chr19	13129273	255	46M	*	0	0	*	*	XA:i:2	MD:Z:25C1T18	NM:i:2	UG:i:387	BX:Z:CCTCG
+SRR2057595.10090939_GATGG	0	chr19	44417644	255	23M	*	0	0	*	*	XA:i:2	MD:Z:15T4C2	NM:i:2	UG:i:601	BX:Z:GATGG
+SRR2057595.10091088_TCGCT	16	chr19	4078298	255	64M	*	0	0	*	*	XA:i:2	MD:Z:42G0A20	NM:i:2	UG:i:67	BX:Z:TCGCT
+SRR2057595.10091189_AGCAT	0	chr19	36976220	255	56M	*	0	0	*	*	XA:i:0	MD:Z:56	NM:i:0	UG:i:539	BX:Z:AGCAT
+SRR2057595.10091303_GTCCA	16	chr19	4078431	255	67M	*	0	0	*	*	XA:i:1	MD:Z:11A55	NM:i:1	UG:i:107	BX:Z:GTCCA
+SRR2057595.10091425_AGCTT	16	chr19	5799262	255	20M	*	0	0	*	*	XA:i:0	MD:Z:20	NM:i:0	UG:i:245	BX:Z:AGCTT
+SRR2057595.10091775_TAGTA	0	chr19	10274608	255	34M	*	0	0	*	*	XA:i:0	MD:Z:34	NM:i:0	UG:i:324	BX:Z:TAGTA
+SRR2057595.10091818_TCTGC	16	chr19	4078299	255	36M	*	0	0	*	*	XA:i:0	MD:Z:36	NM:i:0	UG:i:178	BX:Z:TCTGC
+SRR2057595.10092607_CATAA	0	chr19	13129322	255	35M	*	0	0	*	*	XA:i:1	MD:Z:33C1	NM:i:1	UG:i:401	BX:Z:CATAA
+SRR2057595.10092724_CTGAT	0	chr19	42626015	255	67M	*	0	0	*	*	XA:i:0	MD:Z:67	NM:i:0	UG:i:587	BX:Z:CTGAT
+SRR2057595.10092926_GCTAC	16	chr19	4078446	255	67M	*	0	0	*	*	XA:i:0	MD:Z:67	NM:i:0	UG:i:97	BX:Z:GCTAC
+SRR2057595.1009294_CCAAC	0	chr19	8820047	255	33M	*	0	0	*	*	XA:i:0	MD:Z:33	NM:i:0	UG:i:315	BX:Z:CCAAC
+SRR2057595.10093528_ATACG	0	chr19	13129421	255	31M	*	0	0	*	*	XA:i:1	MD:Z:11A19	NM:i:1	UG:i:393	BX:Z:ATACG
+SRR2057595.1009371_GCGGA	16	chr19	4078298	255	48M	*	0	0	*	*	XA:i:1	MD:Z:43A4	NM:i:1	UG:i:19	BX:Z:GCGGA
+SRR2057595.10094012_GTGGA	16	chr19	35902240	255	30M	*	0	0	*	*	XA:i:0	MD:Z:30	NM:i:0	UG:i:535	BX:Z:GTGGA
+SRR2057595.1009415_TTAAA	16	chr19	4785463	255	21M	*	0	0	*	*	XA:i:0	MD:Z:21	NM:i:0	UG:i:196	BX:Z:TTAAA
+SRR2057595.10094858_ATTAT	0	chr19	4755116	255	67M	*	0	0	*	*	XA:i:2	MD:Z:42C11T12	NM:i:2	UG:i:195	BX:Z:ATTAT
+SRR2057595.10094990_TCCAG	0	chr19	29662201	255	26M	*	0	0	*	*	XA:i:1	MD:Z:0C25	NM:i:1	UG:i:509	BX:Z:TCCAG
+SRR2057595.10095705_AACAG	16	chr19	4078298	255	37M	*	0	0	*	*	XA:i:0	MD:Z:37	NM:i:0	UG:i:174	BX:Z:AACAG
+SRR2057595.10095797_CGTCT	0	chr19	13129329	255	35M	*	0	0	*	*	XA:i:2	MD:Z:26C5A2	NM:i:2	UG:i:421	BX:Z:CGTCT
+SRR2057595.10095837_GCTAT	0	chr19	57701750	255	67M	*	0	0	*	*	XA:i:2	MD:Z:34T1T30	NM:i:2	UG:i:677	BX:Z:GCTAT
+SRR2057595.10096002_AGCCG	16	chr19	4078299	255	36M	*	0	0	*	*	XA:i:0	MD:Z:36	NM:i:0	UG:i:173	BX:Z:AGCCG
+SRR2057595.10096480_TTCGT	16	chr19	40658723	255	67M	*	0	0	*	*	XA:i:1	MD:Z:53T13	NM:i:1	UG:i:576	BX:Z:TTCGT
+SRR2057595.10096536_GCCGA	16	chr19	7389994	255	48M	*	0	0	*	*	XA:i:1	MD:Z:47C0	NM:i:1	UG:i:274	BX:Z:GCCGA
+SRR2057595.10096738_CTAGA	16	chr19	4078299	255	50M	*	0	0	*	*	XA:i:2	MD:Z:41G0A7	NM:i:2	UG:i:43	BX:Z:CTAGA
+SRR2057595.10096905_AATTA	16	chr19	50733890	255	61M	*	0	0	*	*	XA:i:2	MD:Z:55C0C4	NM:i:2	UG:i:645	BX:Z:AATTA
+SRR2057595.10096962_TGTTT	0	chr19	41595518	255	38M	*	0	0	*	*	XA:i:1	MD:Z:25G12	NM:i:1	UG:i:579	BX:Z:TGTTT
+SRR2057595.10096976_AGATC	16	chr19	24352786	255	67M	*	0	0	*	*	XA:i:1	MD:Z:25C41	NM:i:1	UG:i:479	BX:Z:AGATC
+SRR2057595.10097042_CACTG	16	chr19	5798673	255	42M	*	0	0	*	*	XA:i:0	MD:Z:42	NM:i:0	UG:i:249	BX:Z:CACTG
+SRR2057595.10097390_CCATC	0	chr19	5008676	255	30M	*	0	0	*	*	XA:i:2	MD:Z:1A23G4	NM:i:2	UG:i:203	BX:Z:CCATC
+SRR2057595.10097856_TATGG	0	chr19	13129322	255	56M	*	0	0	*	*	XA:i:2	MD:Z:33C5A16	NM:i:2	UG:i:405	BX:Z:TATGG
+SRR2057595.10097927_GCATA	16	chr19	4078298	255	57M	*	0	0	*	*	XA:i:2	MD:Z:42G0A13	NM:i:2	UG:i:53	BX:Z:GCATA
+SRR2057595.10098678_CTCCG	0	chr19	11195966	255	43M	*	0	0	*	*	XA:i:1	MD:Z:0T42	NM:i:1	UG:i:338	BX:Z:CTCCG
+SRR2057595.10098703_TAGTA	0	chr19	10274608	255	34M	*	0	0	*	*	XA:i:0	MD:Z:34	NM:i:0	UG:i:324	BX:Z:TAGTA
+SRR2057595.10098714_GCAGG	0	chr19	47903921	255	21M	*	0	0	*	*	XA:i:2	MD:Z:2T0T17	NM:i:2	UG:i:634	BX:Z:GCAGG
+SRR2057595.10098837_AATTA	16	chr19	50733888	255	63M	*	0	0	*	*	XA:i:2	MD:Z:57C0C4	NM:i:2	UG:i:645	BX:Z:AATTA
+SRR2057595.10098952_AAATC	16	chr19	47891298	255	21M	*	0	0	*	*	XA:i:2	MD:Z:4C11C4	NM:i:2	UG:i:633	BX:Z:AAATC
+SRR2057595.10098_GCCGA	16	chr19	7389994	255	48M	*	0	0	*	*	XA:i:1	MD:Z:47C0	NM:i:1	UG:i:274	BX:Z:GCCGA
+SRR2057595.10099454_GCTAT	0	chr19	57701750	255	67M	*	0	0	*	*	XA:i:2	MD:Z:34T1T30	NM:i:2	UG:i:677	BX:Z:GCTAT
+SRR2057595.10099862_TGATT	16	chr19	4078299	255	50M	*	0	0	*	*	XA:i:2	MD:Z:41G0A7	NM:i:2	UG:i:41	BX:Z:TGATT
+SRR2057595.10099876_AGATC	16	chr19	24352786	255	67M	*	0	0	*	*	XA:i:1	MD:Z:25C41	NM:i:1	UG:i:479	BX:Z:AGATC
+SRR2057595.10100225_GAAGT	16	chr19	8360277	255	67M	*	0	0	*	*	XA:i:2	MD:Z:10A52A3	NM:i:2	UG:i:285	BX:Z:GAAGT
+SRR2057595.10100273_CCTCG	0	chr19	13129273	255	46M	*	0	0	*	*	XA:i:2	MD:Z:25C1T18	NM:i:2	UG:i:387	BX:Z:CCTCG
+SRR2057595.10100361_AGAAT	16	chr19	24246487	255	44M	*	0	0	*	*	XA:i:0	MD:Z:44	NM:i:0	UG:i:476	BX:Z:AGAAT
+SRR2057595.10100419_TCTTG	16	chr19	4078423	255	67M	*	0	0	*	*	XA:i:1	MD:Z:19A47	NM:i:1	UG:i:111	BX:Z:TCTTG
+SRR2057595.10100887_ATACC	16	chr19	35673152	255	21M	*	0	0	*	*	XA:i:2	MD:Z:7T2A10	NM:i:2	UG:i:534	BX:Z:ATACC
+SRR2057595.10101063_ATGAA	0	chr19	56007800	255	67M	*	0	0	*	*	XA:i:0	MD:Z:67	NM:i:0	UG:i:666	BX:Z:ATGAA
+SRR2057595.10101443_TATAT	0	chr19	40194298	255	25M	*	0	0	*	*	XA:i:1	MD:Z:13A11	NM:i:1	UG:i:567	BX:Z:TATAT
+SRR2057595.10101541_TCTAC	0	chr19	8798544	255	51M	*	0	0	*	*	XA:i:0	MD:Z:51	NM:i:0	UG:i:288	BX:Z:TCTAC
+SRR2057595.10101549_AAAGA	0	chr19	42921257	255	44M	*	0	0	*	*	XA:i:2	MD:Z:4G9G29	NM:i:2	UG:i:590	BX:Z:AAAGA
+SRR2057595.10102419_GGGGC	16	chr19	4078446	255	67M	*	0	0	*	*	XA:i:1	MD:Z:66T0	NM:i:1	UG:i:98	BX:Z:GGGGC
+SRR2057595.10102630_CTATT	16	chr19	56874049	255	67M	*	0	0	*	*	XA:i:0	MD:Z:67	NM:i:0	UG:i:672	BX:Z:CTATT
+SRR2057595.10102792_GCTAA	16	chr19	32596026	255	56M	*	0	0	*	*	XA:i:0	MD:Z:56	NM:i:0	UG:i:517	BX:Z:GCTAA
+SRR2057595.10102901_AACTT	16	chr19	4078420	255	64M	*	0	0	*	*	XA:i:2	MD:Z:2T19A41	NM:i:2	UG:i:55	BX:Z:AACTT
+SRR2057595.10102918_ATTAT	16	chr19	7546213	255	27M	*	0	0	*	*	XA:i:0	MD:Z:27	NM:i:0	UG:i:278	BX:Z:ATTAT
+SRR2057595.10103451_GATCA	16	chr19	4078298	255	50M	*	0	0	*	*	XA:i:2	MD:Z:42G0A6	NM:i:2	UG:i:34	BX:Z:GATCA
+SRR2057595.10103512_TCTTG	16	chr19	61240267	255	28M	*	0	0	*	*	XA:i:1	MD:Z:11C16	NM:i:1	UG:i:705	BX:Z:TCTTA
+SRR2057595.10103749_ATCAA	0	chr19	20547357	255	23M	*	0	0	*	*	XA:i:0	MD:Z:23	NM:i:0	UG:i:452	BX:Z:ATCAA
+SRR2057595.10103791_ACGAC	16	chr19	4078298	255	38M	*	0	0	*	*	XA:i:0	MD:Z:38	NM:i:0	UG:i:186	BX:Z:ACGAC
+SRR2057595.10104373_ACAGA	16	chr19	4078299	255	22M	*	0	0	*	*	XA:i:2	MD:Z:0C0C20	NM:i:2	UG:i:136	BX:Z:ACAGA
+SRR2057595.10104410_GCAGG	16	chr19	8963035	255	30M	*	0	0	*	*	XA:i:0	MD:Z:30	NM:i:0	UG:i:317	BX:Z:GCAGG
+SRR2057595.10104500_GTATT	0	chr19	47323072	255	47M	*	0	0	*	*	XA:i:1	MD:Z:0C46	NM:i:1	UG:i:628	BX:Z:GTATT
+SRR2057595.10105258_CGCCG	16	chr19	4078299	255	59M	*	0	0	*	*	XA:i:1	MD:Z:42A16	NM:i:1	UG:i:58	BX:Z:CGCCG
+SRR2057595.10105817_GCGGA	16	chr19	4078298	255	48M	*	0	0	*	*	XA:i:1	MD:Z:43A4	NM:i:1	UG:i:19	BX:Z:GCGGA
+SRR2057595.10105818_AGCCG	16	chr19	4078299	255	36M	*	0	0	*	*	XA:i:1	MD:Z:0C35	NM:i:1	UG:i:173	BX:Z:AGCCG
+SRR2057595.10106002_TTCGC	16	chr19	4078428	255	67M	*	0	0	*	*	XA:i:2	MD:Z:14A24T27	NM:i:2	UG:i:74	BX:Z:TTCGC
+SRR2057595.10106174_CTGCC	16	chr19	3957282	255	22M	*	0	0	*	*	XA:i:2	MD:Z:17C1G2	NM:i:2	UG:i:9	BX:Z:CTGCC
+SRR2057595.10106174_CTGCC	16	chr19	3970897	255	22M	*	0	0	*	*	XA:i:2	MD:Z:17C1G2	NM:i:2	UG:i:10	BX:Z:CTGCC
+SRR2057595.10106212_AGCGC	16	chr19	27464368	255	52M	*	0	0	*	*	XA:i:0	MD:Z:52	NM:i:0	UG:i:493	BX:Z:AGCGC
+SRR2057595.10106528_GCTAT	0	chr19	57701750	255	67M	*	0	0	*	*	XA:i:2	MD:Z:34T1T30	NM:i:2	UG:i:677	BX:Z:GCTAT
+SRR2057595.1010661_ATTAT	16	chr19	7546213	255	27M	*	0	0	*	*	XA:i:0	MD:Z:27	NM:i:0	UG:i:278	BX:Z:ATTAT
+SRR2057595.10106731_CCTCA	0	chr19	8798505	255	48M	*	0	0	*	*	XA:i:0	MD:Z:48	NM:i:0	UG:i:292	BX:Z:CCTCA
+SRR2057595.10106761_CCTCG	0	chr19	13129273	255	46M	*	0	0	*	*	XA:i:2	MD:Z:25C1T18	NM:i:2	UG:i:387	BX:Z:CCTCG
+SRR2057595.10106812_CCAAG	16	chr19	4078298	255	50M	*	0	0	*	*	XA:i:2	MD:Z:42G0A6	NM:i:2	UG:i:37	BX:Z:CCAAG
+SRR2057595.10106892_AGCCG	16	chr19	4078299	255	36M	*	0	0	*	*	XA:i:1	MD:Z:25T10	NM:i:1	UG:i:173	BX:Z:AGCCG
+SRR2057595.10107056_GGCAG	0	chr19	13129322	255	22M	*	0	0	*	*	XA:i:0	MD:Z:22	NM:i:0	UG:i:409	BX:Z:GGCAG
+SRR2057595.1010759_TCTAA	0	chr19	12086447	255	46M	*	0	0	*	*	XA:i:0	MD:Z:46	NM:i:0	UG:i:364	BX:Z:TCTAA
+SRR2057595.1010759_TCTAA	0	chr19	12086782	255	46M	*	0	0	*	*	XA:i:0	MD:Z:46	NM:i:0	UG:i:369	BX:Z:TCTAA
+SRR2057595.10108058_TCTCG	16	chr19	6446235	255	26M	*	0	0	*	*	XA:i:0	MD:Z:26	NM:i:0	UG:i:266	BX:Z:TCTCG
+SRR2057595.10108108_TGTTT	0	chr19	41595519	255	37M	*	0	0	*	*	XA:i:1	MD:Z:24G12	NM:i:1	UG:i:580	BX:Z:TGTTT
+SRR2057595.10108134_TCTTG	16	chr19	4078423	255	67M	*	0	0	*	*	XA:i:1	MD:Z:19A47	NM:i:1	UG:i:111	BX:Z:TCTTG
+SRR2057595.10108322_TATTG	0	chr19	13129322	255	28M	*	0	0	*	*	XA:i:0	MD:Z:28	NM:i:0	UG:i:404	BX:Z:TATTG
+SRR2057595.10108355_TAACA	16	chr19	4078298	255	50M	*	0	0	*	*	XA:i:2	MD:Z:42G0A6	NM:i:2	UG:i:32	BX:Z:TAACA
+SRR2057595.10108853_GGCGT	16	chr19	5797126	255	67M	*	0	0	*	*	XA:i:0	MD:Z:67	NM:i:0	UG:i:233	BX:Z:GGCGT
+SRR2057595.10108990_TTGGC	16	chr19	36085526	255	67M	*	0	0	*	*	XA:i:0	MD:Z:67	NM:i:0	UG:i:536	BX:Z:TTGGC
+SRR2057595.10109290_TATGA	16	chr19	4797429	255	49M	*	0	0	*	*	XA:i:0	MD:Z:49	NM:i:0	UG:i:197	BX:Z:TATGA
+SRR2057595.10109512_CTCTT	16	chr19	10761035	255	32M	*	0	0	*	*	XA:i:0	MD:Z:32	NM:i:0	UG:i:332	BX:Z:CTCTT
+SRR2057595.10109901_ATGAA	16	chr19	4078298	255	37M	*	0	0	*	*	XA:i:0	MD:Z:37	NM:i:0	UG:i:175	BX:Z:ATAAA
+SRR2057595.10110057_TTGTT	16	chr19	61240268	255	24M	*	0	0	*	*	XA:i:1	MD:Z:10C13	NM:i:1	UG:i:696	BX:Z:TTGTT
+SRR2057595.10110159_GCCGA	16	chr19	7389994	255	48M	*	0	0	*	*	XA:i:1	MD:Z:47C0	NM:i:1	UG:i:274	BX:Z:GCCGA
+SRR2057595.10110319_TAGTA	16	chr19	61240268	255	27M	*	0	0	*	*	XA:i:1	MD:Z:10C16	NM:i:1	UG:i:702	BX:Z:TAGTA
+SRR2057595.10110664_TATTG	0	chr19	13129322	255	28M	*	0	0	*	*	XA:i:0	MD:Z:28	NM:i:0	UG:i:404	BX:Z:TATTG
+SRR2057595.10110928_ATCCG	16	chr19	37369602	255	35M	*	0	0	*	*	XA:i:0	MD:Z:35	NM:i:0	UG:i:544	BX:Z:ATCCG
+SRR2057595.10111198_TTCGC	16	chr19	4078428	255	67M	*	0	0	*	*	XA:i:2	MD:Z:14A24T27	NM:i:2	UG:i:74	BX:Z:TTCGC
+SRR2057595.10111819_CCTTT	16	chr19	4078298	255	37M	*	0	0	*	*	XA:i:0	MD:Z:37	NM:i:0	UG:i:180	BX:Z:CCTTT
+SRR2057595.1011210_GCTAC	16	chr19	4078446	255	67M	*	0	0	*	*	XA:i:0	MD:Z:67	NM:i:0	UG:i:97	BX:Z:GCTAC
+SRR2057595.10112142_GATCT	16	chr19	4078433	255	67M	*	0	0	*	*	XA:i:1	MD:Z:9A57	NM:i:1	UG:i:90	BX:Z:GATCT
+SRR2057595.10112493_TCTGT	16	chr19	45885126	255	37M	*	0	0	*	*	XA:i:0	MD:Z:37	NM:i:0	UG:i:608	BX:Z:TCTGT
+SRR2057595.10112719_GATTG	0	chr19	8799830	255	67M	*	0	0	*	*	XA:i:0	MD:Z:67	NM:i:0	UG:i:314	BX:Z:GATTG
+SRR2057595.10113412_TGTTT	0	chr19	41595518	255	38M	*	0	0	*	*	XA:i:1	MD:Z:25G12	NM:i:1	UG:i:579	BX:Z:TGTTT
+SRR2057595.1011350_CATGC	16	chr19	42000773	255	38M	*	0	0	*	*	XA:i:0	MD:Z:38	NM:i:0	UG:i:584	BX:Z:CATGC
+SRR2057595.10113554_TGACC	0	chr19	12086441	255	52M	*	0	0	*	*	XA:i:0	MD:Z:52	NM:i:0	UG:i:362	BX:Z:TGACC
+SRR2057595.10113554_TGACC	0	chr19	12086776	255	52M	*	0	0	*	*	XA:i:0	MD:Z:52	NM:i:0	UG:i:367	BX:Z:TGACC
+SRR2057595.10113832_CTAGA	0	chr19	7609388	255	42M	*	0	0	*	*	XA:i:0	MD:Z:42	NM:i:0	UG:i:279	BX:Z:CTAGA
+SRR2057595.10113868_ATGAA	0	chr19	56007800	255	67M	*	0	0	*	*	XA:i:0	MD:Z:67	NM:i:0	UG:i:666	BX:Z:ATGAA
+SRR2057595.10113897_CATGC	16	chr19	42000773	255	38M	*	0	0	*	*	XA:i:0	MD:Z:38	NM:i:0	UG:i:584	BX:Z:CATGC
+SRR2057595.10114178_TTAAA	16	chr19	4785463	255	21M	*	0	0	*	*	XA:i:0	MD:Z:21	NM:i:0	UG:i:196	BX:Z:TTAAA
+SRR2057595.10114345_GCCGA	16	chr19	7389994	255	48M	*	0	0	*	*	XA:i:1	MD:Z:47C0	NM:i:1	UG:i:274	BX:Z:GCCGA
+SRR2057595.10114548_GATGA	16	chr19	55763541	255	22M	*	0	0	*	*	XA:i:2	MD:Z:2C14G4	NM:i:2	UG:i:662	BX:Z:GATGA
+SRR2057595.10114848_GTTGT	16	chr19	12564367	255	21M	*	0	0	*	*	XA:i:2	MD:Z:0A12A7	NM:i:2	UG:i:380	BX:Z:GTTGT
+SRR2057595.10115148_CTATT	16	chr19	56874049	255	67M	*	0	0	*	*	XA:i:0	MD:Z:67	NM:i:0	UG:i:672	BX:Z:CTATT
+SRR2057595.10115906_TGGAC	16	chr19	4078420	255	67M	*	0	0	*	*	XA:i:2	MD:Z:2T19A44	NM:i:2	UG:i:127	BX:Z:TGGAC
+SRR2057595.10116129_GACTC	16	chr19	25016726	255	31M	*	0	0	*	*	XA:i:0	MD:Z:31	NM:i:0	UG:i:483	BX:Z:GACTC
+SRR2057595.10116129_GACTC	16	chr19	60850233	255	31M	*	0	0	*	*	XA:i:0	MD:Z:31	NM:i:0	UG:i:693	BX:Z:GACTC
+SRR2057595.10116527_CTTAC	16	chr19	4078298	255	54M	*	0	0	*	*	XA:i:2	MD:Z:42G0A10	NM:i:2	UG:i:49	BX:Z:CTTAC
+SRR2057595.10116529_GTCCT	16	chr19	59694502	255	21M	*	0	0	*	*	XA:i:2	MD:Z:8T0A11	NM:i:2	UG:i:688	BX:Z:GTCCT
+SRR2057595.1011687_TAACA	16	chr19	4078298	255	50M	*	0	0	*	*	XA:i:2	MD:Z:42G0A6	NM:i:2	UG:i:32	BX:Z:TAACA
+SRR2057595.10116904_TTTTC	16	chr19	4078296	255	67M	*	0	0	*	*	XA:i:2	MD:Z:0T44A21	NM:i:2	UG:i:69	BX:Z:TTTTC
+SRR2057595.10117105_CGCTT	0	chr19	13129322	255	26M	*	0	0	*	*	XA:i:0	MD:Z:26	NM:i:0	UG:i:406	BX:Z:CGCTT
+SRR2057595.10117768_TATCA	16	chr19	60837460	255	67M	*	0	0	*	*	XA:i:0	MD:Z:67	NM:i:0	UG:i:691	BX:Z:TATCA
+SRR2057595.1011778_TGTTT	0	chr19	41595518	255	38M	*	0	0	*	*	XA:i:1	MD:Z:25G12	NM:i:1	UG:i:579	BX:Z:TGTTT
+SRR2057595.10117819_CTAGC	16	chr19	4078298	255	62M	*	0	0	*	*	XA:i:2	MD:Z:21C21A18	NM:i:2	UG:i:63	BX:Z:CTAGC
+SRR2057595.10117947_AATCG	16	chr19	4078303	255	67M	*	0	0	*	*	XA:i:2	MD:Z:38A21A6	NM:i:2	UG:i:88	BX:Z:AATCG
+SRR2057595.10118173_TTCGT	0	chr19	36922761	255	57M	*	0	0	*	*	XA:i:1	MD:Z:5C51	NM:i:1	UG:i:538	BX:Z:TTCGT
+SRR2057595.10118916_AGCCG	16	chr19	4078299	255	36M	*	0	0	*	*	XA:i:0	MD:Z:36	NM:i:0	UG:i:173	BX:Z:AGCCG
+SRR2057595.10119454_TGTTT	0	chr19	41595518	255	38M	*	0	0	*	*	XA:i:1	MD:Z:25G12	NM:i:1	UG:i:579	BX:Z:TGTTT
+SRR2057595.10119776_GTCAA	16	chr19	4078298	255	57M	*	0	0	*	*	XA:i:1	MD:Z:43A13	NM:i:1	UG:i:52	BX:Z:GTCAA
+SRR2057595.10119820_CAGAC	0	chr19	5493782	255	41M	*	0	0	*	*	XA:i:0	MD:Z:41	NM:i:0	UG:i:218	BX:Z:CAGAC
+SRR2057595.10119919_GAATG	16	chr19	33191616	255	28M	*	0	0	*	*	XA:i:2	MD:Z:1G17G8	NM:i:2	UG:i:526	BX:Z:GAATG
+SRR2057595.10120218_TGGTA	16	chr19	28085954	255	38M	*	0	0	*	*	XA:i:0	MD:Z:38	NM:i:0	UG:i:498	BX:Z:TGGTA
+SRR2057595.10120257_TCAGG	0	chr19	13129322	255	35M	*	0	0	*	*	XA:i:1	MD:Z:33C1	NM:i:1	UG:i:407	BX:Z:TCAGG
+SRR2057595.10120271_GTCCC	0	chr19	12347657	255	32M	*	0	0	*	*	XA:i:0	MD:Z:32	NM:i:0	UG:i:372	BX:Z:GTCCC
+SRR2057595.10120271_GTCCC	0	chr19	12348043	255	32M	*	0	0	*	*	XA:i:0	MD:Z:32	NM:i:0	UG:i:374	BX:Z:GTCCC
+SRR2057595.10120271_GTCCC	16	chr19	15035071	255	32M	*	0	0	*	*	XA:i:1	MD:Z:29T2	NM:i:1	UG:i:428	BX:Z:GTCCC
+SRR2057595.10120377_TATTC	16	chr19	5038295	255	24M	*	0	0	*	*	XA:i:0	MD:Z:24	NM:i:0	UG:i:206	BX:Z:TATTC
+SRR2057595.10120569_GCATA	16	chr19	4078298	255	57M	*	0	0	*	*	XA:i:2	MD:Z:42G0A13	NM:i:2	UG:i:53	BX:Z:GCATA
+SRR2057595.10120734_AGTAA	16	chr19	4078423	255	67M	*	0	0	*	*	XA:i:2	MD:Z:19A24T22	NM:i:2	UG:i:110	BX:Z:AGTAA
+SRR2057595.10120758_AACGC	16	chr19	4078298	255	51M	*	0	0	*	*	XA:i:1	MD:Z:43A7	NM:i:1	UG:i:42	BX:Z:AACGC
+SRR2057595.10120905_ACCTG	16	chr19	4078420	255	67M	*	0	0	*	*	XA:i:2	MD:Z:2T19A44	NM:i:2	UG:i:126	BX:Z:ACCTG
+SRR2057595.10121117_TGTTC	0	chr19	61241963	255	27M	*	0	0	*	*	XA:i:2	MD:Z:20A5G0	NM:i:2	UG:i:719	BX:Z:TGTTC
+SRR2057595.10121638_AATTA	16	chr19	50733888	255	63M	*	0	0	*	*	XA:i:2	MD:Z:57C0C4	NM:i:2	UG:i:645	BX:Z:AATTA
+SRR2057595.10122214_TATTG	0	chr19	13129322	255	28M	*	0	0	*	*	XA:i:0	MD:Z:28	NM:i:0	UG:i:404	BX:Z:TATTG
+SRR2057595.10122328_TAAAG	16	chr19	61240268	255	28M	*	0	0	*	*	XA:i:1	MD:Z:10C17	NM:i:1	UG:i:710	BX:Z:TAAAG
+SRR2057595.10122369_AGCTT	16	chr19	5799262	255	20M	*	0	0	*	*	XA:i:0	MD:Z:20	NM:i:0	UG:i:245	BX:Z:AGCTT
+SRR2057595.10122580_AAGGT	0	chr19	8798514	255	39M	*	0	0	*	*	XA:i:0	MD:Z:39	NM:i:0	UG:i:294	BX:Z:AAGGT
+SRR2057595.101226_AACTT	16	chr19	4078420	255	64M	*	0	0	*	*	XA:i:2	MD:Z:2T19A41	NM:i:2	UG:i:55	BX:Z:AACTT
+SRR2057595.10123331_ATTAT	16	chr19	7546213	255	27M	*	0	0	*	*	XA:i:0	MD:Z:27	NM:i:0	UG:i:278	BX:Z:ATTAT
+SRR2057595.10123377_CTAAT	0	chr19	40194243	255	44M	*	0	0	*	*	XA:i:2	MD:Z:5T20A17	NM:i:2	UG:i:562	BX:Z:CTAAT
+SRR2057595.1012355_CAACT	16	chr19	4078298	255	49M	*	0	0	*	*	XA:i:2	MD:Z:42G0A5	NM:i:2	UG:i:25	BX:Z:CAACT
+SRR2057595.10123626_TCTAC	0	chr19	8798544	255	51M	*	0	0	*	*	XA:i:0	MD:Z:51	NM:i:0	UG:i:288	BX:Z:TCTAC
+SRR2057595.10124059_GATGA	16	chr19	55763541	255	22M	*	0	0	*	*	XA:i:2	MD:Z:2C14G4	NM:i:2	UG:i:662	BX:Z:GATGA
+SRR2057595.1012426_CATTT	0	chr19	49059158	255	49M	*	0	0	*	*	XA:i:2	MD:Z:30A7A10	NM:i:2	UG:i:639	BX:Z:CATTT
+SRR2057595.10125107_TATGG	0	chr19	13129322	255	56M	*	0	0	*	*	XA:i:2	MD:Z:33C5A16	NM:i:2	UG:i:405	BX:Z:TATGG
+SRR2057595.1012543_GGATC	16	chr19	4078298	255	61M	*	0	0	*	*	XA:i:2	MD:Z:42G0A17	NM:i:2	UG:i:61	BX:Z:GGATC
+SRR2057595.10125580_TGCTA	0	chr19	10830415	255	21M	*	0	0	*	*	XA:i:2	MD:Z:0A10C9	NM:i:2	UG:i:334	BX:Z:TGCTA
+SRR2057595.1012559_TGATT	16	chr19	4078299	255	50M	*	0	0	*	*	XA:i:2	MD:Z:41G0A7	NM:i:2	UG:i:41	BX:Z:TGATT
+SRR2057595.10125877_CTATT	16	chr19	56874049	255	67M	*	0	0	*	*	XA:i:0	MD:Z:67	NM:i:0	UG:i:672	BX:Z:CTATT
+SRR2057595.10125930_ATCGG	0	chr19	8799853	255	44M	*	0	0	*	*	XA:i:0	MD:Z:44	NM:i:0	UG:i:310	BX:Z:ATCGG
+SRR2057595.10125966_GATGA	16	chr19	55763541	255	22M	*	0	0	*	*	XA:i:2	MD:Z:2C14G4	NM:i:2	UG:i:662	BX:Z:GATGA
+SRR2057595.10126475_TCAGG	0	chr19	38669715	255	54M	*	0	0	*	*	XA:i:0	MD:Z:54	NM:i:0	UG:i:550	BX:Z:TCAGG
+SRR2057595.10126817_CAGAC	0	chr19	5493782	255	41M	*	0	0	*	*	XA:i:0	MD:Z:41	NM:i:0	UG:i:218	BX:Z:CAGAC
+SRR2057595.1012699_TTTTC	16	chr19	4078296	255	67M	*	0	0	*	*	XA:i:2	MD:Z:0T44A21	NM:i:2	UG:i:69	BX:Z:TTTTC
+SRR2057595.10127386_GCAGA	0	chr19	8799844	255	53M	*	0	0	*	*	XA:i:0	MD:Z:53	NM:i:0	UG:i:309	BX:Z:GCAGA
+SRR2057595.10127482_AAGGT	0	chr19	8798514	255	39M	*	0	0	*	*	XA:i:0	MD:Z:39	NM:i:0	UG:i:294	BX:Z:AAGGT
+SRR2057595.10127633_GGACC	16	chr19	4078298	255	28M	*	0	0	*	*	XA:i:1	MD:Z:18C9	NM:i:1	UG:i:143	BX:Z:GGACC
+SRR2057595.10127800_CCTTT	16	chr19	4078298	255	37M	*	0	0	*	*	XA:i:0	MD:Z:37	NM:i:0	UG:i:180	BX:Z:CCTTT
+SRR2057595.10127806_TTTTC	16	chr19	4078296	255	67M	*	0	0	*	*	XA:i:2	MD:Z:0T44A21	NM:i:2	UG:i:69	BX:Z:TTTTC
+SRR2057595.10127925_GCCGA	16	chr19	7389994	255	48M	*	0	0	*	*	XA:i:1	MD:Z:47C0	NM:i:1	UG:i:274	BX:Z:GCCGA
+SRR2057595.10127969_TCAGC	16	chr19	4078298	255	49M	*	0	0	*	*	XA:i:1	MD:Z:43A5	NM:i:1	UG:i:23	BX:Z:TCAGC
+SRR2057595.10128074_TATTC	16	chr19	5038295	255	24M	*	0	0	*	*	XA:i:0	MD:Z:24	NM:i:0	UG:i:206	BX:Z:TATTC
+SRR2057595.10128154_CTAGG	16	chr19	5798663	255	67M	*	0	0	*	*	XA:i:2	MD:Z:0G0G65	NM:i:2	UG:i:242	BX:Z:CTAGG
+SRR2057595.10128297_ATAAA	16	chr19	4078298	255	37M	*	0	0	*	*	XA:i:0	MD:Z:37	NM:i:0	UG:i:175	BX:Z:ATAAA
+SRR2057595.10128465_TTGCA	0	chr19	44434953	255	43M	*	0	0	*	*	XA:i:0	MD:Z:43	NM:i:0	UG:i:602	BX:Z:TTGCA
+SRR2057595.10128496_AGCCG	16	chr19	4078298	255	39M	*	0	0	*	*	XA:i:0	MD:Z:39	NM:i:0	UG:i:11	BX:Z:AGCCG
+SRR2057595.1012868_AAACA	16	chr19	4078299	255	49M	*	0	0	*	*	XA:i:1	MD:Z:42A6	NM:i:1	UG:i:33	BX:Z:AAACA
+SRR2057595.1012871_TTCGC	16	chr19	4078428	255	67M	*	0	0	*	*	XA:i:2	MD:Z:14A24T27	NM:i:2	UG:i:74	BX:Z:TTCGC
+SRR2057595.10128720_CCTCA	0	chr19	8798505	255	48M	*	0	0	*	*	XA:i:0	MD:Z:48	NM:i:0	UG:i:292	BX:Z:CCTCA
+SRR2057595.10128783_TTGCA	0	chr19	44434953	255	43M	*	0	0	*	*	XA:i:0	MD:Z:43	NM:i:0	UG:i:602	BX:Z:TTGCA
+SRR2057595.10128887_ATACC	16	chr19	35673152	255	21M	*	0	0	*	*	XA:i:2	MD:Z:7T2A10	NM:i:2	UG:i:534	BX:Z:ATACC
+SRR2057595.10128931_AGCCG	16	chr19	4078299	255	36M	*	0	0	*	*	XA:i:0	MD:Z:36	NM:i:0	UG:i:173	BX:Z:AGCCG
+SRR2057595.10129033_ACAGA	16	chr19	4078299	255	22M	*	0	0	*	*	XA:i:2	MD:Z:0C0C20	NM:i:2	UG:i:136	BX:Z:ACAGA
+SRR2057595.10129046_TCAAA	16	chr19	4078298	255	38M	*	0	0	*	*	XA:i:0	MD:Z:38	NM:i:0	UG:i:185	BX:Z:TCAAA
+SRR2057595.10129405_ATGAA	16	chr19	4078298	255	37M	*	0	0	*	*	XA:i:0	MD:Z:37	NM:i:0	UG:i:175	BX:Z:ATAAA
+SRR2057595.1012950_GGGAT	0	chr19	13129424	255	25M	*	0	0	*	*	XA:i:1	MD:Z:8A16	NM:i:1	UG:i:394	BX:Z:GGGAT
+SRR2057595.10129521_CGCCG	16	chr19	4078299	255	59M	*	0	0	*	*	XA:i:1	MD:Z:42A16	NM:i:1	UG:i:58	BX:Z:CGCCG
+SRR2057595.10129734_TATTG	0	chr19	13129322	255	28M	*	0	0	*	*	XA:i:0	MD:Z:28	NM:i:0	UG:i:404	BX:Z:TATTG
+SRR2057595.10130199_GGCAG	0	chr19	13129322	255	22M	*	0	0	*	*	XA:i:0	MD:Z:22	NM:i:0	UG:i:409	BX:Z:GGCAG
+SRR2057595.10130333_GTCGC	0	chr19	12080548	255	51M	*	0	0	*	*	XA:i:2	MD:Z:28C20G1	NM:i:2	UG:i:345	BX:Z:GTCGC
+SRR2057595.10130333_GTCGC	16	chr19	12084189	255	51M	*	0	0	*	*	XA:i:2	MD:Z:1C20G28	NM:i:2	UG:i:352	BX:Z:GTCGC
+SRR2057595.10130767_ATACC	16	chr19	35673152	255	21M	*	0	0	*	*	XA:i:2	MD:Z:7T2A10	NM:i:2	UG:i:534	BX:Z:ATACC
+SRR2057595.10130878_TTAGT	16	chr19	4078302	255	67M	*	0	0	*	*	XA:i:2	MD:Z:39A21A5	NM:i:2	UG:i:81	BX:Z:TTAGT
+SRR2057595.10131031_AGCTT	16	chr19	5799262	255	20M	*	0	0	*	*	XA:i:0	MD:Z:20	NM:i:0	UG:i:245	BX:Z:AGCTT
+SRR2057595.10131148_CAGCA	16	chr19	5801892	255	47M	*	0	0	*	*	XA:i:0	MD:Z:47	NM:i:0	UG:i:258	BX:Z:CAGCA
+SRR2057595.10131269_CATGC	0	chr19	13129570	255	22M	*	0	0	*	*	XA:i:1	MD:Z:9A12	NM:i:1	UG:i:392	BX:Z:CATGC
+SRR2057595.10131482_CTTTC	16	chr19	4078298	255	53M	*	0	0	*	*	XA:i:2	MD:Z:42G0A9	NM:i:2	UG:i:47	BX:Z:CTTTC
+SRR2057595.10132002_CCCGT	16	chr19	47349292	255	19M	*	0	0	*	*	XA:i:2	MD:Z:8A3G6	NM:i:2	UG:i:629	BX:Z:CCCGT
+SRR2057595.10132034_ATACC	16	chr19	35673152	255	21M	*	0	0	*	*	XA:i:2	MD:Z:7T2A10	NM:i:2	UG:i:534	BX:Z:ATACC
+SRR2057595.10132108_TCTAC	0	chr19	8798544	255	51M	*	0	0	*	*	XA:i:0	MD:Z:51	NM:i:0	UG:i:288	BX:Z:TCTAC
+SRR2057595.10132494_CGAAC	0	chr19	13129273	255	36M	*	0	0	*	*	XA:i:2	MD:Z:25C1T8	NM:i:2	UG:i:386	BX:Z:CGAAC
+SRR2057595.10133086_TCTTA	16	chr19	61240268	255	27M	*	0	0	*	*	XA:i:1	MD:Z:10C16	NM:i:1	UG:i:705	BX:Z:TCTTA
+SRR2057595.10133357_GTCGC	0	chr19	12080548	255	51M	*	0	0	*	*	XA:i:2	MD:Z:28C20G1	NM:i:2	UG:i:345	BX:Z:GTCGC
+SRR2057595.10133357_GTCGC	16	chr19	12084189	255	51M	*	0	0	*	*	XA:i:2	MD:Z:1C20G28	NM:i:2	UG:i:352	BX:Z:GTCGC
+SRR2057595.1013336_TTGTT	16	chr19	61240268	255	24M	*	0	0	*	*	XA:i:1	MD:Z:10C13	NM:i:1	UG:i:696	BX:Z:TTGTT
+SRR2057595.10133381_AGAGT	16	chr19	24180225	255	55M	*	0	0	*	*	XA:i:1	MD:Z:41C13	NM:i:1	UG:i:474	BX:Z:AGAGT
+SRR2057595.10133440_CTGCG	16	chr19	61240305	255	36M	*	0	0	*	*	XA:i:2	MD:Z:17A4T13	NM:i:2	UG:i:712	BX:Z:CTGCG
+SRR2057595.10134016_GACAC	0	chr19	12348541	255	26M	*	0	0	*	*	XA:i:0	MD:Z:26	NM:i:0	UG:i:378	BX:Z:GACAC
+SRR2057595.10134016_GACAC	16	chr19	15034579	255	26M	*	0	0	*	*	XA:i:0	MD:Z:26	NM:i:0	UG:i:429	BX:Z:GACAC
+SRR2057595.10134072_GCAGG	16	chr19	8963035	255	30M	*	0	0	*	*	XA:i:0	MD:Z:30	NM:i:0	UG:i:317	BX:Z:GCAGG
+SRR2057595.1013452_ATATA	0	chr19	13129330	255	22M	*	0	0	*	*	XA:i:1	MD:Z:0T21	NM:i:1	UG:i:422	BX:Z:ATATA
+SRR2057595.10134979_AGTAT	0	chr19	10600420	255	29M	*	0	0	*	*	XA:i:0	MD:Z:29	NM:i:0	UG:i:328	BX:Z:AGTAT
+SRR2057595.10135304_GTCCA	16	chr19	4078431	255	67M	*	0	0	*	*	XA:i:1	MD:Z:11A55	NM:i:1	UG:i:107	BX:Z:GTCCA
+SRR2057595.1013548_TGTTT	16	chr19	50526830	255	51M	*	0	0	*	*	XA:i:0	MD:Z:51	NM:i:0	UG:i:643	BX:Z:TGTTT
+SRR2057595.10135758_TTGCA	0	chr19	44434953	255	43M	*	0	0	*	*	XA:i:0	MD:Z:43	NM:i:0	UG:i:602	BX:Z:TTGCA
+SRR2057595.10135807_CCCGT	16	chr19	47349292	255	19M	*	0	0	*	*	XA:i:2	MD:Z:8A3G6	NM:i:2	UG:i:629	BX:Z:CCCGT
+SRR2057595.1013613_GATTG	0	chr19	55501486	255	31M	*	0	0	*	*	XA:i:1	MD:Z:8A22	NM:i:1	UG:i:659	BX:Z:GATTG
+SRR2057595.1013616_ATAGA	0	chr19	38795805	255	62M	*	0	0	*	*	XA:i:1	MD:Z:46A15	NM:i:1	UG:i:553	BX:Z:ATAGA
+SRR2057595.10136267_TTCGC	16	chr19	4078428	255	67M	*	0	0	*	*	XA:i:2	MD:Z:14A24T27	NM:i:2	UG:i:74	BX:Z:TTCGC
+SRR2057595.10136714_ATCCG	16	chr19	37369602	255	35M	*	0	0	*	*	XA:i:0	MD:Z:35	NM:i:0	UG:i:544	BX:Z:ATCCG
+SRR2057595.10136765_GCTAT	0	chr19	57701750	255	67M	*	0	0	*	*	XA:i:2	MD:Z:34T1T30	NM:i:2	UG:i:677	BX:Z:GCTAT
+SRR2057595.10137151_ATTCT	0	chr19	3576314	255	23M	*	0	0	*	*	XA:i:2	MD:Z:16T5T0	NM:i:2	UG:i:5	BX:Z:ATTCT
+SRR2057595.10137262_ACAAA	16	chr19	4078298	255	49M	*	0	0	*	*	XA:i:1	MD:Z:43A5	NM:i:1	UG:i:27	BX:Z:ACAAA
+SRR2057595.10137317_GATTG	0	chr19	8799830	255	67M	*	0	0	*	*	XA:i:0	MD:Z:67	NM:i:0	UG:i:314	BX:Z:GATTG
+SRR2057595.10137603_GCTAT	16	chr19	61240267	255	28M	*	0	0	*	*	XA:i:1	MD:Z:11C16	NM:i:1	UG:i:706	BX:Z:GCTAT
+SRR2057595.10137788_TAGTA	0	chr19	10274608	255	34M	*	0	0	*	*	XA:i:0	MD:Z:34	NM:i:0	UG:i:324	BX:Z:TAGTA
+SRR2057595.1013793_GCGCA	0	chr19	8798523	255	30M	*	0	0	*	*	XA:i:0	MD:Z:30	NM:i:0	UG:i:295	BX:Z:GCGCA
+SRR2057595.10138029_GTTGT	16	chr19	12564367	255	21M	*	0	0	*	*	XA:i:2	MD:Z:0A12A7	NM:i:2	UG:i:380	BX:Z:GTTGT
+SRR2057595.10138656_CTAGC	16	chr19	4078298	255	62M	*	0	0	*	*	XA:i:1	MD:Z:43A18	NM:i:1	UG:i:63	BX:Z:CTAGC
+SRR2057595.10138697_GCCGT	0	chr19	30861987	255	20M	*	0	0	*	*	XA:i:2	MD:Z:3C2T13	NM:i:2	UG:i:511	BX:Z:GCCGT
+SRR2057595.10138764_GATGG	0	chr19	44417644	255	23M	*	0	0	*	*	XA:i:2	MD:Z:15T4C2	NM:i:2	UG:i:601	BX:Z:GATGG
+SRR2057595.10138897_TGATT	16	chr19	4078299	255	50M	*	0	0	*	*	XA:i:2	MD:Z:41G0A7	NM:i:2	UG:i:41	BX:Z:TGATT
+SRR2057595.10139239_GAGTG	0	chr19	8798502	255	51M	*	0	0	*	*	XA:i:0	MD:Z:51	NM:i:0	UG:i:289	BX:Z:GAGTG
+SRR2057595.10139499_AGTCT	0	chr19	13129558	255	37M	*	0	0	*	*	XA:i:1	MD:Z:21A15	NM:i:1	UG:i:398	BX:Z:AGTCT
+SRR2057595.10139596_GCTAC	16	chr19	4078446	255	67M	*	0	0	*	*	XA:i:0	MD:Z:67	NM:i:0	UG:i:97	BX:Z:GCTAC
+SRR2057595.10139652_GGATG	0	chr19	13129322	255	27M	*	0	0	*	*	XA:i:0	MD:Z:27	NM:i:0	UG:i:402	BX:Z:GGATG
+SRR2057595.10139674_ATGAA	16	chr19	4078298	255	37M	*	0	0	*	*	XA:i:0	MD:Z:37	NM:i:0	UG:i:175	BX:Z:ATAAA
+SRR2057595.10139952_TAGTA	16	chr19	61240268	255	27M	*	0	0	*	*	XA:i:1	MD:Z:10C16	NM:i:1	UG:i:702	BX:Z:TAGTA
+SRR2057595.10140058_ATATA	0	chr19	13129330	255	22M	*	0	0	*	*	XA:i:1	MD:Z:0T21	NM:i:1	UG:i:422	BX:Z:ATATA
+SRR2057595.10141122_GTCCC	0	chr19	12347657	255	32M	*	0	0	*	*	XA:i:0	MD:Z:32	NM:i:0	UG:i:372	BX:Z:GTCCC
+SRR2057595.10141122_GTCCC	0	chr19	12348043	255	32M	*	0	0	*	*	XA:i:0	MD:Z:32	NM:i:0	UG:i:374	BX:Z:GTCCC
+SRR2057595.10141122_GTCCC	16	chr19	15035071	255	32M	*	0	0	*	*	XA:i:1	MD:Z:29T2	NM:i:1	UG:i:428	BX:Z:GTCCC
+SRR2057595.10141295_TAGTA	0	chr19	10274608	255	34M	*	0	0	*	*	XA:i:0	MD:Z:34	NM:i:0	UG:i:324	BX:Z:TAGTA
+SRR2057595.10142441_GTCTA	16	chr19	4078420	255	67M	*	0	0	*	*	XA:i:2	MD:Z:2T19A44	NM:i:2	UG:i:124	BX:Z:GTCTA
+SRR2057595.10142482_GCCGA	16	chr19	7389994	255	48M	*	0	0	*	*	XA:i:1	MD:Z:47C0	NM:i:1	UG:i:274	BX:Z:GCCGA
+SRR2057595.10143102_AAGTA	16	chr19	31326011	255	57M	*	0	0	*	*	XA:i:1	MD:Z:23A33	NM:i:1	UG:i:512	BX:Z:AAGTA
+SRR2057595.10143387_GCATA	16	chr19	4078298	255	57M	*	0	0	*	*	XA:i:2	MD:Z:42G0A13	NM:i:2	UG:i:53	BX:Z:GCATA
+SRR2057595.10143631_ATTTT	0	chr19	45095257	255	57M	*	0	0	*	*	XA:i:0	MD:Z:57	NM:i:0	UG:i:606	BX:Z:ATTTT
+SRR2057595.10143687_CGCTT	0	chr19	13129322	255	26M	*	0	0	*	*	XA:i:0	MD:Z:26	NM:i:0	UG:i:406	BX:Z:CGCTT
+SRR2057595.10143772_TGATT	16	chr19	4078299	255	50M	*	0	0	*	*	XA:i:2	MD:Z:41G0A7	NM:i:2	UG:i:41	BX:Z:TGATT
+SRR2057595.10143909_CAGAC	0	chr19	5493782	255	41M	*	0	0	*	*	XA:i:0	MD:Z:41	NM:i:0	UG:i:218	BX:Z:CAGAC
+SRR2057595.10143972_CTTAC	16	chr19	4078298	255	54M	*	0	0	*	*	XA:i:2	MD:Z:42G0A10	NM:i:2	UG:i:49	BX:Z:CTTAC
+SRR2057595.10144216_CATAA	0	chr19	13129322	255	35M	*	0	0	*	*	XA:i:1	MD:Z:33C1	NM:i:1	UG:i:401	BX:Z:CATAA
+SRR2057595.10144367_ATGAT	16	chr19	61240306	255	36M	*	0	0	*	*	XA:i:2	MD:Z:16A4T14	NM:i:2	UG:i:716	BX:Z:ATGAT
Binary file test-data/group_in2.bam has changed
Binary file test-data/group_in3.bam has changed
Binary file test-data/group_in4.bam has changed
Binary file test-data/group_in5.bam has changed
Binary file test-data/group_in6.bam has changed
Binary file test-data/group_out2.bam has changed
Binary file test-data/group_out3.bam has changed
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/group_out3.tab	Wed Jan 10 19:09:42 2018 -0500
@@ -0,0 +1,498 @@
+read_id	contig	position	gene	umi	umi_count	final_umi	final_umi_count	unique_id
+chrM_81_583_3:0:0_2:0:0_f4/1_TT	chrM	80	NA	TT	1	TT	1	0
+chrM_110_732_3:0:0_2:0:0_160/1_CC	chrM	109	NA	CC	1	CC	1	1
+chrM_118_613_6:0:0_4:0:0_169/1_AG	chrM	117	NA	AG	1	AG	1	2
+chrM_149_684_2:0:0_2:0:0_6e/1_TA	chrM	148	NA	TA	1	TA	1	3
+chrM_152_616_6:0:0_2:0:0_84/1_GC	chrM	151	NA	GC	1	GC	1	4
+chrM_247_748_8:0:0_1:0:0_1b7/1_GC	chrM	246	NA	GC	1	GC	1	5
+chrM_280_772_1:0:0_9:0:0_31/1_CG	chrM	279	NA	CG	1	CG	1	6
+chrM_292_819_2:0:0_1:0:0_18d/1_CA	chrM	291	NA	CA	1	CA	1	7
+chrM_390_890_3:0:0_6:0:0_bf/1_CA	chrM	389	NA	CA	1	CA	1	8
+chrM_447_921_2:0:0_3:0:0_1cc/1_AC	chrM	446	NA	AC	1	AC	1	9
+chrM_469_983_2:0:0_1:0:0_121/1_AC	chrM	468	NA	AC	1	AC	1	10
+chrM_541_1074_3:0:0_8:1:0_22/1_AC	chrM	540	NA	AC	1	AC	1	11
+chrM_8_556_3:0:0_2:0:0_1b1/1_AC	chrM	556	NA	AC	1	AC	1	12
+chrM_112_577_2:0:0_4:0:0_17b/1_CG	chrM	577	NA	CG	1	CG	1	13
+chrM_627_1063_6:0:0_4:1:0_12a/1_AA	chrM	626	NA	AA	1	AA	1	14
+chrM_164_650_5:0:0_3:0:0_164/1_AC	chrM	650	NA	AC	1	AC	1	15
+chrM_200_695_6:0:0_2:0:0_5a/1_TA	chrM	695	NA	TA	3	TA	3	16
+chrM_168_695_5:0:0_2:0:0_1af/1_TA	chrM	695	NA	TA	3	TA	3	16
+chrM_170_695_1:0:0_6:0:0_1e3/1_TA	chrM	695	NA	TA	3	TA	3	16
+chrM_209_705_3:0:0_2:0:0_1b3/1_TA	chrM	705	NA	TA	1	TA	1	17
+chrM_288_807_3:0:0_5:0:0_8e/1_AG	chrM	807	NA	AG	1	AG	1	18
+chrM_818_1274_1:1:0_4:0:0_124/1_TC	chrM	817	NA	TC	1	TC	1	19
+chrM_442_872_4:0:0_6:0:0_146/1_AT	chrM	872	NA	AT	1	AT	1	20
+chrM_460_919_3:0:0_1:0:0_0/1_GA	chrM	919	NA	GA	1	GA	1	21
+chrM_384_950_5:0:0_2:0:0_34/1_GG	chrM	950	NA	GG	1	GG	1	22
+chrM_476_980_5:0:0_2:0:0_133/1_TA	chrM	980	NA	TA	1	TA	1	23
+chrM_552_999_4:0:0_5:0:0_bb/1_AT	chrM	999	NA	AT	1	AT	1	24
+chrM_512_1030_5:0:0_7:1:0_a5/1_AC	chrM	1030	NA	AC	1	AC	1	25
+chrM_1103_1634_5:0:0_3:0:0_36/1_TA	chrM	1102	NA	TA	1	TA	1	26
+chrM_648_1148_3:0:0_3:1:0_1d1/1_TT	chrM	1148	NA	TT	1	TT	1	27
+chrM_1167_1797_8:0:0_2:1:0_14e/1_GT	chrM	1166	NA	GT	1	GT	1	28
+chrM_619_1169_3:0:0_5:1:0_33/1_CC	chrM	1169	NA	CC	1	CC	1	29
+chrM_668_1248_0:0:0_4:0:0_fa/1_TT	chrM	1248	NA	TT	1	TT	1	30
+chrM_1288_1759_4:0:0_2:1:0_a1/1_TA	chrM	1287	NA	TA	1	TA	1	31
+chrM_1327_1786_4:0:0_3:1:0_1ad/1_CA	chrM	1326	NA	CA	1	CA	1	32
+chrM_866_1351_5:1:0_6:0:0_1c9/1_TA	chrM	1351	NA	TA	1	TA	1	33
+chrM_853_1366_6:1:0_4:0:0_13b/1_TC	chrM	1366	NA	TC	1	TC	1	34
+chrM_1399_1851_2:0:0_11:1:0_85/1_AT	chrM	1398	NA	AT	1	AT	1	35
+chrM_946_1444_4:1:0_6:0:0_113/1_TT	chrM	1444	NA	TT	1	TT	1	36
+chrM_943_1485_2:1:0_5:0:0_1e2/1_AT	chrM	1485	NA	AT	1	AT	1	37
+chrM_1022_1501_4:0:0_5:0:0_132/1_GA	chrM	1501	NA	GA	1	GA	1	38
+chrM_1504_2013_4:1:0_3:0:0_10b/1_AT	chrM	1503	NA	AT	1	AT	1	39
+chrM_1505_1934_1:1:0_5:0:0_157/1_TC	chrM	1504	NA	TC	1	TC	1	40
+chrM_997_1511_2:1:0_1:0:0_1d8/1_AT	chrM	1511	NA	AT	1	AT	1	41
+chrM_1521_2070_5:1:0_3:0:0_1a/1_TA	chrM	1520	NA	TA	1	TA	1	42
+chrM_1575_2126_5:1:0_2:1:0_18c/1_TA	chrM	1574	NA	TA	1	TA	1	43
+chrM_1063_1598_5:0:0_5:0:0_f5/1_TG	chrM	1598	NA	TG	1	TG	1	44
+chrM_1605_2128_3:1:0_4:1:0_1ea/1_TT	chrM	1604	NA	TT	1	TT	1	45
+chrM_1065_1609_3:0:0_5:0:0_18e/1_TA	chrM	1609	NA	TA	1	TA	1	46
+chrM_1140_1619_1:0:0_4:0:0_74/1_TT	chrM	1619	NA	TT	1	TT	1	47
+chrM_1111_1626_4:0:0_2:0:0_186/1_AC	chrM	1626	NA	AC	1	AC	1	48
+chrM_1664_2135_0:1:0_3:1:0_179/1_TT	chrM	1663	NA	TT	1	TT	1	49
+chrM_1699_2147_1:0:0_7:0:0_10c/1_AA	chrM	1698	NA	AA	1	AA	1	50
+chrM_1706_2240_3:0:0_6:0:0_99/1_GT	chrM	1705	NA	GT	1	GT	1	51
+chrM_1756_2309_0:0:0_3:0:0_c3/1_AT	chrM	1755	NA	AT	1	AT	1	52
+chrM_1344_1758_4:0:0_3:1:0_75/1_TT	chrM	1758	NA	TT	1	TT	1	53
+chrM_1223_1777_5:0:0_6:1:0_42/1_TG	chrM	1777	NA	TG	1	TG	1	54
+chrM_1790_2351_4:0:0_4:0:0_f3/1_CC	chrM	1789	NA	CC	1	CC	1	55
+chrM_1308_1807_4:0:0_4:1:0_45/1_TA	chrM	1807	NA	TA	1	TA	1	56
+chrM_1814_2315_6:0:0_3:0:0_12d/1_AA	chrM	1813	NA	AA	1	AA	1	57
+chrM_1862_2304_4:0:0_1:0:0_19d/1_AA	chrM	1861	NA	AA	1	AA	1	58
+chrM_1363_1869_5:0:0_3:1:0_aa/1_TA	chrM	1869	NA	TA	1	TA	1	59
+chrM_1363_1887_2:0:0_3:0:0_d3/1_CA	chrM	1887	NA	CA	1	CA	1	60
+chrM_1428_1904_7:0:0_1:0:0_1b0/1_AC	chrM	1904	NA	AC	1	AC	1	61
+chrM_1480_1975_4:0:0_6:0:0_cd/1_CT	chrM	1975	NA	CT	1	CT	1	62
+chrM_1998_2552_3:0:0_2:0:0_35/1_AC	chrM	1997	NA	AC	1	AC	1	63
+chrM_1427_2035_6:0:0_1:0:0_7d/1_TA	chrM	2035	NA	TA	1	TA	1	64
+chrM_2065_2529_4:1:0_2:0:0_69/1_CG	chrM	2064	NA	CG	1	CG	1	65
+chrM_2079_2546_5:1:0_2:0:0_37/1_TA	chrM	2078	NA	TA	1	TA	1	66
+chrM_1576_2102_2:1:0_1:0:0_3/1_CT	chrM	2102	NA	CT	1	CT	1	67
+chrM_2118_2553_2:0:0_6:0:0_e6/1_CC	chrM	2117	NA	CC	1	CC	1	68
+chrM_1707_2180_3:0:0_2:0:0_152/1_TT	chrM	2180	NA	TT	1	TT	1	69
+chrM_1950_2421_3:0:0_3:0:0_13/1_TC	chrM	2421	NA	TC	1	TC	1	70
+chrM_2426_2953_5:0:0_5:0:0_83/1_AT	chrM	2425	NA	AT	1	AT	1	71
+chrM_2497_3082_1:0:0_6:0:0_1a4/1_AG	chrM	2496	NA	AG	1	AG	1	72
+chrM_2021_2503_3:1:0_4:0:0_4d/1_AC	chrM	2503	NA	AC	1	AC	1	73
+chrM_2510_3002_4:0:0_1:0:0_1d/1_AA	chrM	2509	NA	AA	1	AA	1	74
+chrM_2528_3123_6:1:0_4:0:0_122/1_AT	chrM	2527	NA	AT	1	AT	1	75
+chrM_2646_3108_4:1:0_3:0:0_68/1_GA	chrM	2645	NA	GA	1	GA	1	76
+chrM_2063_2659_1:1:0_8:0:0_93/1_GG	chrM	2659	NA	GG	1	GG	1	77
+chrM_2160_2679_2:0:0_4:0:0_177/1_TC	chrM	2679	NA	TC	1	TC	1	78
+chrM_2823_3302_7:0:0_9:0:0_de/1_CC	chrM	2822	NA	CC	1	CC	1	79
+chrM_2441_2926_5:0:0_3:0:0_25/1_AA	chrM	2926	NA	AA	1	AA	1	80
+chrM_2958_3490_6:0:0_5:1:0_192/1_TT	chrM	2957	NA	TT	1	TT	1	81
+chrM_2988_3473_2:0:0_4:1:0_c6/1_GT	chrM	2987	NA	GT	1	GT	1	82
+chrM_3032_3426_3:0:0_3:1:0_17c/1_AG	chrM	3031	NA	AG	1	AG	1	83
+chrM_2563_3061_1:1:0_3:0:0_70/1_GG	chrM	3061	NA	GG	1	GG	1	84
+chrM_2692_3152_1:1:0_4:0:0_5d/1_TC	chrM	3152	NA	TC	1	TC	1	85
+chrM_2612_3153_2:1:0_1:0:0_1df/1_AT	chrM	3153	NA	AT	1	AT	1	86
+chrM_3202_3692_4:0:0_2:0:0_95/1_AA	chrM	3201	NA	AA	1	AA	1	87
+chrM_2733_3206_4:0:0_1:0:0_4e/1_GT	chrM	3206	NA	GT	1	GT	1	88
+chrM_2706_3256_4:0:0_9:0:0_16e/1_GA	chrM	3256	NA	GA	1	GA	1	89
+chrM_2864_3323_4:0:0_5:0:0_fb/1_CT	chrM	3323	NA	CT	1	CT	1	90
+chrM_2856_3324_2:0:0_6:0:0_4/1_GC	chrM	3324	NA	GC	1	GC	1	91
+chrM_2902_3333_5:0:0_3:0:0_4a/1_CC	chrM	3333	NA	CC	1	CC	1	92
+chrM_2890_3410_3:0:0_6:1:0_1b5/1_TA	chrM	3410	NA	TA	1	TA	1	93
+chrM_2875_3422_5:0:0_7:1:0_c5/1_GG	chrM	3422	NA	GG	1	GG	1	94
+chrM_2890_3430_4:0:0_8:1:0_19a/1_TT	chrM	3430	NA	TT	1	TT	1	95
+chrM_2949_3443_10:0:0_6:1:0_139/1_CT	chrM	3443	NA	CT	1	CT	1	96
+chrM_2872_3457_8:0:0_3:1:0_134/1_TG	chrM	3457	NA	TG	1	TG	1	97
+chrM_2948_3460_5:0:0_7:1:0_1c1/1_TG	chrM	3460	NA	TG	1	TG	1	98
+chrM_3015_3496_4:0:0_5:1:0_137/1_AT	chrM	3496	NA	AT	1	AT	1	99
+chrM_2965_3521_2:0:0_2:1:0_eb/1_TA	chrM	3521	NA	TA	1	TA	1	100
+chrM_3561_4058_2:0:0_2:0:0_14d/1_TG	chrM	3560	NA	TG	1	TG	1	101
+chrM_3590_4068_6:0:0_6:1:0_b8/1_GG	chrM	3589	NA	GG	1	GG	1	102
+chrM_3123_3605_5:0:0_4:0:0_14/1_AG	chrM	3605	NA	AG	1	AG	1	103
+chrM_3158_3635_7:0:0_0:0:0_1c/1_AG	chrM	3635	NA	AG	1	AG	1	104
+chrM_3253_3640_6:1:0_8:0:0_10a/1_GT	chrM	3640	NA	GT	1	GT	1	105
+chrM_3663_4221_4:0:0_1:0:0_1a9/1_TA	chrM	3662	NA	TA	1	TA	1	106
+chrM_3679_4214_3:1:0_4:0:0_54/1_AG	chrM	3678	NA	AG	1	AG	1	107
+chrM_3688_4175_3:1:0_8:0:0_fd/1_AA	chrM	3687	NA	AA	1	AA	1	108
+chrM_3216_3691_4:1:0_4:0:0_110/1_TT	chrM	3691	NA	TT	1	TT	1	109
+chrM_3701_4211_4:0:0_3:0:0_e0/1_TA	chrM	3700	NA	TA	1	TA	1	110
+chrM_3213_3703_4:1:0_6:0:0_199/1_AG	chrM	3703	NA	AG	1	AG	1	111
+chrM_3738_4170_4:1:0_3:0:0_3b/1_CA	chrM	3737	NA	CA	1	CA	1	112
+chrM_3752_4208_9:1:0_5:0:0_11f/1_CA	chrM	3751	NA	CA	1	CA	1	113
+chrM_3171_3761_4:0:0_6:0:0_38/1_AT	chrM	3761	NA	AT	1	AT	1	114
+chrM_3213_3785_3:1:0_10:0:0_ed/1_GT	chrM	3785	NA	GT	1	GT	1	115
+chrM_3793_4298_6:0:0_5:0:0_51/1_TC	chrM	3792	NA	TC	1	TC	1	116
+chrM_3805_4310_4:1:0_3:0:0_161/1_TA	chrM	3804	NA	TA	1	TA	1	117
+chrM_3817_4309_3:1:0_3:0:0_86/1_AT	chrM	3816	NA	AT	1	AT	1	118
+chrM_3863_4305_5:0:0_5:0:0_e9/1_AC	chrM	3862	NA	AC	1	AC	1	119
+chrM_3399_3957_5:1:0_2:1:0_43/1_AT	chrM	3957	NA	AT	1	AT	1	120
+chrM_3449_3959_6:0:0_3:1:0_16a/1_GA	chrM	3959	NA	GA	1	GA	1	121
+chrM_3971_4563_4:0:0_5:0:0_150/1_GG	chrM	3970	NA	GG	1	GG	1	122
+chrM_3541_3989_4:0:0_2:1:0_90/1_GA	chrM	3989	NA	GA	1	GA	1	123
+chrM_3597_3996_3:0:0_7:0:0_62/1_GT	chrM	3996	NA	GT	1	GT	1	124
+chrM_3450_3998_6:0:0_2:0:0_1ba/1_GC	chrM	3998	NA	GC	1	GC	1	125
+chrM_3637_4061_5:0:0_2:1:0_111/1_AT	chrM	4061	NA	AT	1	AT	1	126
+chrM_4068_4556_3:0:0_3:0:0_6a/1_CA	chrM	4067	NA	CA	1	CA	1	127
+chrM_4074_4533_2:0:0_2:0:0_9e/1_TA	chrM	4073	NA	TA	1	TA	1	128
+chrM_4082_4621_7:0:0_3:0:0_198/1_AA	chrM	4081	NA	AA	1	AA	1	129
+chrM_4160_4692_5:0:0_4:1:0_1f0/1_AT	chrM	4159	NA	AT	1	AT	1	130
+chrM_4199_4751_2:0:0_1:1:0_15c/1_GC	chrM	4198	NA	GC	1	GC	1	131
+chrM_4229_4769_4:0:0_6:0:0_cc/1_GA	chrM	4228	NA	GA	1	GA	1	132
+chrM_3770_4283_1:0:0_8:0:0_13f/1_AA	chrM	4283	NA	AA	1	AA	1	133
+chrM_4374_4823_2:0:0_5:0:0_dc/1_TA	chrM	4373	NA	TA	1	TA	1	134
+chrM_4382_4881_2:0:0_5:0:0_195/1_AT	chrM	4381	NA	AT	1	AT	1	135
+chrM_4384_4922_5:0:0_4:1:0_1e1/1_TA	chrM	4383	NA	TA	1	TA	1	136
+chrM_4056_4522_5:0:0_4:0:0_e3/1_AT	chrM	4522	NA	AT	1	AT	1	137
+chrM_4553_5058_6:0:0_4:0:0_a3/1_AT	chrM	4552	NA	AT	1	AT	1	138
+chrM_4565_5190_3:0:0_4:0:0_19c/1_AC	chrM	4564	NA	AC	1	AC	1	139
+chrM_4106_4574_6:0:0_2:0:0_64/1_TC	chrM	4574	NA	TC	1	TC	1	140
+chrM_4705_5072_2:0:0_2:1:0_b7/1_TT	chrM	4704	NA	TT	1	TT	1	141
+chrM_4724_5279_5:0:0_8:0:0_2e/1_AA	chrM	4723	NA	AA	1	AA	1	142
+chrM_4840_5344_4:1:0_4:0:0_3c/1_GT	chrM	4839	NA	GT	1	GT	1	143
+chrM_4509_4974_5:0:0_3:0:0_ea/1_CT	chrM	4974	NA	CT	1	CT	1	144
+chrM_4444_5010_5:1:0_5:1:0_119/1_TG	chrM	5010	NA	TG	1	TG	1	145
+chrM_4491_5013_5:1:0_6:1:0_1b/1_GA	chrM	5013	NA	GA	1	GA	1	146
+chrM_4490_5017_8:0:0_4:0:0_8d/1_AG	chrM	5017	NA	AG	1	AG	1	147
+chrM_5058_5566_6:0:0_5:0:0_1c6/1_AG	chrM	5057	NA	AG	1	AG	1	148
+chrM_5062_5522_7:0:0_5:0:0_e8/1_TC	chrM	5061	NA	TC	1	TC	1	149
+chrM_4566_5086_5:1:0_6:1:0_19/1_AT	chrM	5086	NA	AT	1	AT	1	150
+chrM_5119_5706_5:0:0_4:0:0_140/1_TG	chrM	5118	NA	TG	1	TG	1	151
+chrM_5121_5635_3:0:0_3:0:0_165/1_AG	chrM	5120	NA	AG	1	AG	1	152
+chrM_5166_5697_4:0:0_4:0:0_ec/1_TC	chrM	5165	NA	TC	1	TC	1	153
+chrM_4583_5175_2:0:0_4:0:0_10d/1_TT	chrM	5175	NA	TT	1	TT	1	154
+chrM_5184_5616_6:0:0_4:0:0_1ee/1_AA	chrM	5183	NA	AA	1	AA	1	155
+chrM_4713_5198_5:0:0_1:0:0_f0/1_TA	chrM	5198	NA	TA	1	TA	1	156
+chrM_4833_5239_3:1:0_3:0:0_56/1_AG	chrM	5239	NA	AG	1	AG	1	157
+chrM_5292_5797_3:0:0_3:0:0_130/1_CC	chrM	5291	NA	CC	1	CC	1	158
+chrM_5315_5855_2:0:0_5:0:0_136/1_TA	chrM	5314	NA	TA	1	TA	1	159
+chrM_5342_5909_5:0:0_5:0:0_5/1_GC	chrM	5341	NA	GC	1	GC	1	160
+chrM_4886_5348_5:0:0_3:0:0_d7/1_TC	chrM	5348	NA	TC	1	TC	1	161
+chrM_5355_5797_0:0:0_5:0:0_1d3/1_TA	chrM	5354	NA	TA	1	TA	1	162
+chrM_5362_5890_4:0:0_3:0:0_1dd/1_AT	chrM	5361	NA	AT	1	AT	1	163
+chrM_4974_5463_3:0:0_4:0:0_8/1_CT	chrM	5463	NA	CT	1	CT	1	164
+chrM_5125_5611_2:0:0_2:0:0_11b/1_AT	chrM	5611	NA	AT	1	AT	1	165
+chrM_5163_5634_8:0:0_3:0:0_1bf/1_TT	chrM	5634	NA	TT	1	TT	1	166
+chrM_5185_5662_3:0:0_4:0:0_a4/1_AT	chrM	5662	NA	AT	1	AT	1	167
+chrM_5683_6115_5:0:0_5:0:0_b5/1_CT	chrM	5682	NA	CT	1	CT	1	168
+chrM_5306_5717_1:0:0_4:0:0_1ab/1_TT	chrM	5717	NA	TT	1	TT	1	169
+chrM_5181_5752_4:0:0_4:0:0_128/1_GT	chrM	5752	NA	GT	1	GT	1	170
+chrM_5828_6286_5:0:0_4:0:0_17f/1_TT	chrM	5827	NA	TT	1	TT	1	171
+chrM_5310_5842_3:0:0_6:0:0_1d0/1_TA	chrM	5842	NA	TA	1	TA	1	172
+chrM_5845_6332_2:0:0_3:0:0_db/1_AA	chrM	5844	NA	AA	1	AA	1	173
+chrM_5856_6361_4:0:0_4:0:0_1d4/1_AA	chrM	5855	NA	AA	1	AA	1	174
+chrM_5410_5865_9:0:0_5:0:0_57/1_TA	chrM	5865	NA	TA	1	TA	1	175
+chrM_5411_5901_3:0:0_2:0:0_197/1_TT	chrM	5901	NA	TT	1	TT	1	176
+chrM_5423_5960_2:0:0_6:0:0_1ca/1_TA	chrM	5960	NA	TA	1	TA	1	177
+chrM_5461_5962_4:0:0_8:0:0_1a8/1_AT	chrM	5962	NA	AT	1	AT	1	178
+chrM_5990_6598_5:0:0_2:0:0_16f/1_TC	chrM	5989	NA	TC	1	TC	1	179
+chrM_6047_6502_2:0:0_6:0:0_1f3/1_CA	chrM	6046	NA	CA	1	CA	1	180
+chrM_6078_6610_7:0:0_6:0:0_29/1_GG	chrM	6077	NA	GG	1	GG	1	181
+chrM_5565_6084_3:0:0_3:0:0_194/1_TC	chrM	6084	NA	TC	1	TC	1	182
+chrM_6087_6574_4:0:0_2:0:0_12e/1_CC	chrM	6086	NA	CC	1	CC	1	183
+chrM_5710_6256_2:0:0_6:0:0_12/1_CT	chrM	6256	NA	CT	2	CT	2	184
+chrM_5852_6256_2:0:0_4:0:0_58/1_CT	chrM	6256	NA	CT	2	CT	2	184
+chrM_5770_6275_1:0:0_3:0:0_a6/1_AG	chrM	6275	NA	AG	1	AG	1	185
+chrM_5839_6288_5:0:0_5:0:0_1c0/1_GG	chrM	6288	NA	GG	1	GG	1	186
+chrM_5829_6293_3:0:0_1:0:0_bd/1_GC	chrM	6293	NA	GC	1	GC	1	187
+chrM_5793_6362_4:0:0_5:0:0_1a5/1_CC	chrM	6362	NA	CC	1	CC	1	188
+chrM_5942_6425_5:0:0_1:0:0_16d/1_AC	chrM	6425	NA	AC	1	AC	1	189
+chrM_5989_6468_5:0:0_1:0:0_16c/1_TG	chrM	6468	NA	TG	1	TG	1	190
+chrM_5994_6492_3:0:0_3:0:0_153/1_TA	chrM	6492	NA	TA	1	TA	1	191
+chrM_6011_6514_3:0:0_7:0:0_1ef/1_TG	chrM	6514	NA	TG	1	TG	1	192
+chrM_6134_6610_4:0:0_2:0:0_d6/1_GG	chrM	6610	NA	GG	1	GG	1	193
+chrM_6073_6613_4:0:0_6:0:0_e/1_CT	chrM	6613	NA	CT	1	CT	1	194
+chrM_6655_7146_3:0:0_6:0:0_55/1_TT	chrM	6654	NA	TT	1	TT	1	195
+chrM_6155_6684_4:0:0_6:0:0_156/1_GG	chrM	6684	NA	GG	1	GG	1	196
+chrM_6698_7198_5:0:1_4:0:0_19e/1_AT	chrM	6697	NA	AT	1	AT	1	197
+chrM_6222_6744_5:0:0_2:0:0_116/1_CA	chrM	6744	NA	CA	1	CA	1	198
+chrM_6258_6750_3:0:0_2:0:0_b0/1_TT	chrM	6750	NA	TT	1	TT	1	199
+chrM_6331_6811_6:0:0_5:0:0_b6/1_GT	chrM	6811	NA	GT	1	GT	1	200
+chrM_6370_6852_5:0:0_4:0:0_5c/1_AT	chrM	6852	NA	AT	1	AT	1	201
+chrM_6507_6920_3:0:0_5:0:1_1d2/1_AA	chrM	6920	NA	AA	1	AA	1	202
+chrM_6479_6981_2:0:0_5:0:1_18f/1_TG	chrM	6981	NA	TG	1	TG	1	203
+chrM_6530_7006_2:0:0_2:0:1_101/1_TA	chrM	7006	NA	TA	1	TA	1	204
+chrM_7048_7515_9:0:0_4:0:0_7e/1_AT	chrM	7047	NA	AT	1	AT	1	205
+chrM_7081_7575_3:0:0_0:0:0_10/1_CC	chrM	7080	NA	CC	1	CC	1	206
+chrM_7119_7629_5:0:0_7:0:0_1cf/1_GC	chrM	7118	NA	GC	1	GC	1	207
+chrM_7270_7705_10:0:0_4:0:0_b4/1_GC	chrM	7269	NA	GC	1	GC	1	208
+chrM_7272_7803_5:0:0_4:0:0_fe/1_TA	chrM	7271	NA	TA	1	TA	1	209
+chrM_6787_7349_2:0:1_1:0:0_141/1_TG	chrM	7349	NA	TG	1	TG	1	210
+chrM_7365_7794_3:0:0_5:0:0_1c7/1_GA	chrM	7364	NA	GA	1	GA	1	211
+chrM_7413_7973_6:0:0_5:0:0_158/1_TC	chrM	7412	NA	TC	1	TC	1	212
+chrM_6786_7414_0:0:1_6:0:0_1b6/1_AT	chrM	7414	NA	AT	1	AT	1	213
+chrM_7472_7886_4:0:0_4:0:0_8b/1_GG	chrM	7471	NA	GG	1	GG	1	214
+chrM_7505_7965_4:0:0_2:0:0_3a/1_CT	chrM	7504	NA	CT	1	CT	1	215
+chrM_7095_7547_2:0:0_3:0:0_149/1_GG	chrM	7547	NA	GG	1	GG	1	216
+chrM_7564_8021_3:0:0_2:0:0_1/1_AA	chrM	7563	NA	AA	1	AA	1	217
+chrM_7067_7564_4:0:0_5:0:0_107/1_AA	chrM	7564	NA	AA	1	AA	1	218
+chrM_7016_7567_4:0:0_5:0:0_27/1_TT	chrM	7567	NA	TT	1	TT	1	219
+chrM_7200_7687_2:0:0_6:0:0_c/1_TA	chrM	7687	NA	TA	1	TA	1	220
+chrM_7267_7793_1:0:0_4:0:0_41/1_AC	chrM	7793	NA	AC	1	AC	1	221
+chrM_7797_8230_4:0:0_5:0:0_f6/1_GC	chrM	7796	NA	GC	1	GC	1	222
+chrM_7821_8285_3:0:0_3:0:0_1f1/1_TT	chrM	7820	NA	TT	1	TT	1	223
+chrM_7859_8392_2:0:0_3:0:0_114/1_TT	chrM	7858	NA	TT	1	TT	1	224
+chrM_7881_8395_3:0:0_2:0:0_173/1_CT	chrM	7880	NA	CT	1	CT	1	225
+chrM_7339_7893_4:0:0_3:0:0_19b/1_TA	chrM	7893	NA	TA	1	TA	1	226
+chrM_7424_7912_5:0:0_4:0:0_ba/1_GG	chrM	7912	NA	GG	1	GG	1	227
+chrM_7467_7949_6:0:0_9:0:0_7f/1_AT	chrM	7949	NA	AT	1	AT	1	228
+chrM_7956_8469_8:0:0_5:1:0_14a/1_AA	chrM	7955	NA	AA	1	AA	1	229
+chrM_7963_8508_6:0:0_5:1:0_73/1_AC	chrM	7962	NA	AC	1	AC	1	230
+chrM_8018_8547_2:0:0_2:1:0_39/1_TA	chrM	8017	NA	TA	1	TA	1	231
+chrM_8039_8593_3:0:0_3:1:0_71/1_AG	chrM	8038	NA	AG	1	AG	1	232
+chrM_7594_8044_3:0:0_5:0:0_e1/1_TT	chrM	8044	NA	TT	1	TT	1	233
+chrM_7570_8062_4:0:0_1:0:0_1ed/1_TC	chrM	8062	NA	TC	1	TC	1	234
+chrM_7576_8099_6:0:0_4:0:0_1bc/1_GT	chrM	8099	NA	GT	1	GT	1	235
+chrM_8114_8625_4:0:0_3:1:0_17a/1_AT	chrM	8113	NA	AT	1	AT	1	236
+chrM_7655_8131_5:0:0_7:0:0_19f/1_CG	chrM	8131	NA	CG	1	CG	1	237
+chrM_8139_8619_2:0:0_5:1:0_166/1_CC	chrM	8138	NA	CC	1	CC	1	238
+chrM_7825_8191_3:0:0_2:0:0_1ec/1_CT	chrM	8191	NA	CT	1	CT	1	239
+chrM_7641_8206_4:0:0_5:0:0_13a/1_GT	chrM	8206	NA	GT	1	GT	1	240
+chrM_7613_8208_2:0:0_5:0:0_52/1_AT	chrM	8208	NA	AT	1	AT	1	241
+chrM_7752_8261_10:0:0_5:0:0_142/1_GA	chrM	8261	NA	GA	1	GA	1	242
+chrM_7739_8294_7:0:0_2:0:0_144/1_CC	chrM	8294	NA	CC	1	CC	1	243
+chrM_8303_8710_5:1:0_6:0:0_87/1_CA	chrM	8302	NA	CA	1	CA	1	244
+chrM_8309_8842_3:1:0_5:0:0_115/1_GA	chrM	8308	NA	GA	1	GA	1	245
+chrM_7965_8408_4:0:0_0:0:0_143/1_CC	chrM	8408	NA	CC	1	CC	1	246
+chrM_8031_8436_7:0:0_5:0:0_c0/1_TC	chrM	8436	NA	TC	1	TC	1	247
+chrM_8438_8973_3:1:0_2:0:0_1d5/1_AA	chrM	8437	NA	AA	1	AA	1	248
+chrM_8511_8920_4:0:0_3:0:0_ca/1_AT	chrM	8510	NA	AT	1	AT	1	249
+chrM_8532_8954_3:0:0_4:0:0_cb/1_TT	chrM	8531	NA	TT	1	TT	1	250
+chrM_8565_8973_6:0:0_1:0:0_66/1_GA	chrM	8564	NA	GA	1	GA	1	251
+chrM_8575_9003_7:0:0_2:0:0_c7/1_TA	chrM	8574	NA	TA	1	TA	1	252
+chrM_8125_8594_2:0:0_2:1:0_187/1_TA	chrM	8594	NA	TA	1	TA	1	253
+chrM_8144_8650_4:0:0_3:1:0_14b/1_GG	chrM	8650	NA	GG	1	GG	1	254
+chrM_8117_8669_3:0:0_5:0:0_16b/1_AG	chrM	8669	NA	AG	1	AG	1	255
+chrM_8296_8802_4:1:0_9:0:0_1db/1_GC	chrM	8802	NA	GC	1	GC	1	256
+chrM_8807_9277_3:0:0_4:0:1_108/1_TG	chrM	8806	NA	TG	1	TG	1	257
+chrM_8825_9275_3:0:0_5:0:1_1da/1_AA	chrM	8824	NA	AA	1	AA	1	258
+chrM_8868_9400_4:0:0_3:0:1_1e8/1_AC	chrM	8867	NA	AC	1	AC	1	259
+chrM_8885_9340_4:0:0_5:0:1_c8/1_TT	chrM	8884	NA	TT	1	TT	1	260
+chrM_8938_9405_8:0:0_2:0:1_1c3/1_CG	chrM	8937	NA	CG	1	CG	1	261
+chrM_8396_8947_8:1:0_2:0:0_155/1_AC	chrM	8947	NA	AC	1	AC	1	262
+chrM_8461_8951_7:0:0_1:0:0_1cb/1_TG	chrM	8951	NA	TG	1	TG	1	263
+chrM_8987_9581_3:0:0_3:0:0_1cd/1_TA	chrM	8986	NA	TA	1	TA	1	264
+chrM_9007_9456_2:0:0_4:0:0_23/1_AA	chrM	9006	NA	AA	1	AA	1	265
+chrM_8520_9009_2:0:0_7:0:0_193/1_TA	chrM	9009	NA	TA	1	TA	1	266
+chrM_8547_9016_5:0:0_4:0:0_32/1_TG	chrM	9016	NA	TG	1	TG	1	267
+chrM_9075_9551_5:0:1_3:0:0_4b/1_CC	chrM	9074	NA	CC	1	CC	1	268
+chrM_9102_9561_3:0:1_3:0:0_2b/1_TA	chrM	9101	NA	TA	1	TA	1	269
+chrM_9113_9686_4:0:1_1:0:0_78/1_CA	chrM	9112	NA	CA	1	CA	1	270
+chrM_8621_9133_8:0:0_5:0:0_145/1_GC	chrM	9133	NA	GC	1	GC	1	271
+chrM_9166_9651_6:0:1_4:0:0_15b/1_TT	chrM	9165	NA	TT	1	TT	1	272
+chrM_9168_9638_4:0:1_5:0:0_175/1_TC	chrM	9167	NA	TC	1	TC	1	273
+chrM_9172_9645_0:0:1_5:0:0_e7/1_CA	chrM	9171	NA	CA	1	CA	1	274
+chrM_9233_9739_3:0:1_5:0:0_91/1_CT	chrM	9232	NA	CT	1	CT	1	275
+chrM_9249_9749_6:0:1_2:0:0_12c/1_TA	chrM	9247	NA	TA	1	TA	1	276
+chrM_9338_9899_1:0:0_2:0:0_a8/1_TT	chrM	9337	NA	TT	1	TT	1	277
+chrM_8914_9385_2:0:0_3:0:1_135/1_GC	chrM	9385	NA	GC	1	GC	1	278
+chrM_8829_9414_6:0:0_2:0:1_1bd/1_GT	chrM	9414	NA	GT	1	GT	1	279
+chrM_8916_9457_5:0:0_5:0:0_167/1_AC	chrM	9457	NA	AC	1	AC	1	280
+chrM_8970_9471_5:0:0_6:0:0_1ac/1_CT	chrM	9471	NA	CT	1	CT	1	281
+chrM_8928_9477_3:0:0_6:0:0_48/1_GG	chrM	9477	NA	GG	2	GG	2	282
+chrM_8929_9477_7:0:0_5:0:0_f9/1_GG	chrM	9477	NA	GG	2	GG	2	282
+chrM_9536_10046_5:0:0_1:0:0_d5/1_TA	chrM	9535	NA	TA	1	TA	1	283
+chrM_9579_10013_2:0:0_3:0:0_9/1_AC	chrM	9578	NA	AC	1	AC	1	284
+chrM_9672_10188_5:0:0_9:0:0_11/1_CG	chrM	9671	NA	CG	1	CG	1	285
+chrM_9689_10220_2:0:0_2:0:0_151/1_AA	chrM	9688	NA	AA	1	AA	1	286
+chrM_9159_9700_2:0:1_4:0:0_2/1_GA	chrM	9700	NA	GA	1	GA	1	287
+chrM_9103_9709_4:0:1_5:0:0_24/1_AT	chrM	9709	NA	AT	1	AT	1	288
+chrM_9768_10236_5:0:0_4:0:0_1d9/1_GT	chrM	9767	NA	GT	1	GT	1	289
+chrM_9271_9836_3:0:0_2:0:0_77/1_CT	chrM	9836	NA	CT	1	CT	1	290
+chrM_9847_10370_4:0:0_5:0:0_bc/1_CA	chrM	9846	NA	CA	1	CA	1	291
+chrM_9856_10428_4:0:0_4:1:0_120/1_GC	chrM	9855	NA	GC	1	GC	1	292
+chrM_9866_10380_3:0:0_5:0:0_8f/1_CC	chrM	9865	NA	CC	1	CC	1	293
+chrM_9895_10412_1:0:0_7:1:0_7a/1_AT	chrM	9894	NA	AT	1	AT	1	294
+chrM_9904_10380_7:0:0_3:0:0_1e0/1_TG	chrM	9903	NA	TG	1	TG	1	295
+chrM_9917_10459_5:0:0_6:0:0_9c/1_CG	chrM	9916	NA	CG	1	CG	1	296
+chrM_9489_9919_2:0:0_2:0:0_b9/1_GG	chrM	9919	NA	GG	1	GG	1	297
+chrM_9452_9927_4:0:0_5:0:0_190/1_GA	chrM	9927	NA	GA	1	GA	1	298
+chrM_9932_10484_3:0:0_5:0:0_148/1_CT	chrM	9931	NA	CT	1	CT	1	299
+chrM_9983_10519_5:0:0_2:0:0_dd/1_AC	chrM	9982	NA	AC	1	AC	1	300
+chrM_9402_10032_4:0:0_5:0:0_a2/1_GG	chrM	10032	NA	GG	1	GG	1	301
+chrM_10074_10591_1:0:0_0:0:0_14c/1_TA	chrM	10073	NA	TA	1	TA	1	302
+chrM_10103_10594_8:0:0_6:1:0_1e9/1_AT	chrM	10102	NA	AT	1	AT	1	303
+chrM_10141_10683_4:0:0_4:0:0_6f/1_TA	chrM	10140	NA	TA	1	TA	1	304
+chrM_10226_10692_5:1:0_4:0:0_103/1_CT	chrM	10225	NA	CT	1	CT	1	305
+chrM_9669_10227_5:0:0_4:0:0_4f/1_TG	chrM	10227	NA	TG	1	TG	1	306
+chrM_9735_10257_0:0:0_7:0:0_b2/1_TA	chrM	10257	NA	TA	1	TA	1	307
+chrM_10326_10838_5:0:0_3:0:0_f2/1_TT	chrM	10325	NA	TT	1	TT	1	308
+chrM_9825_10334_2:0:0_6:0:0_123/1_AC	chrM	10334	NA	AC	1	AC	1	309
+chrM_10009_10476_0:0:0_5:0:0_176/1_CA	chrM	10476	NA	CA	1	CA	1	310
+chrM_9995_10574_6:0:0_4:1:0_7c/1_GG	chrM	10574	NA	GG	1	GG	1	311
+chrM_10582_11133_4:0:0_3:0:0_21/1_GC	chrM	10581	NA	GC	1	GC	1	312
+chrM_10590_11016_2:0:0_6:0:0_81/1_AA	chrM	10589	NA	AA	1	AA	1	313
+chrM_10607_11150_5:0:0_2:0:0_8a/1_CC	chrM	10606	NA	CC	1	CC	1	314
+chrM_10041_10610_6:0:0_4:0:0_ef/1_GG	chrM	10610	NA	GG	1	GG	1	315
+chrM_10679_11090_2:0:0_6:0:0_1b4/1_GA	chrM	10678	NA	GA	1	GA	1	316
+chrM_10690_11211_4:0:0_3:0:0_184/1_AC	chrM	10689	NA	AC	1	AC	1	317
+chrM_10692_11227_2:0:0_4:0:0_49/1_TG	chrM	10691	NA	TG	1	TG	1	318
+chrM_10325_10814_5:1:0_6:0:0_129/1_GC	chrM	10814	NA	GC	1	GC	1	319
+chrM_10834_11259_4:0:0_3:0:0_47/1_GA	chrM	10833	NA	GA	1	GA	1	320
+chrM_10846_11368_1:0:0_6:0:0_fc/1_TG	chrM	10845	NA	TG	1	TG	1	321
+chrM_10850_11370_7:0:0_4:0:0_15e/1_TT	chrM	10849	NA	TT	1	TT	1	322
+chrM_10491_10996_4:0:0_6:0:0_1a6/1_AG	chrM	10996	NA	AG	1	AG	1	323
+chrM_11001_11542_4:0:0_9:0:0_15/1_GT	chrM	11000	NA	GT	1	GT	1	324
+chrM_11067_11640_2:0:0_7:0:0_d2/1_TT	chrM	11066	NA	TT	1	TT	1	325
+chrM_10600_11115_3:0:0_3:0:0_10f/1_TT	chrM	11115	NA	TT	1	TT	1	326
+chrM_11179_11645_6:0:1_1:0:0_181/1_TC	chrM	11178	NA	TC	1	TC	1	327
+chrM_10716_11197_6:0:0_5:0:0_67/1_CA	chrM	11197	NA	CA	1	CA	1	328
+chrM_10649_11218_5:0:0_8:0:0_50/1_GT	chrM	11218	NA	GT	1	GT	1	329
+chrM_10624_11233_2:0:0_3:0:0_2f/1_CT	chrM	11233	NA	CT	1	CT	1	330
+chrM_11239_11740_6:0:0_1:0:0_3e/1_CT	chrM	11238	NA	CT	1	CT	1	331
+chrM_10825_11310_5:0:0_6:0:0_1ce/1_TA	chrM	11310	NA	TA	1	TA	1	332
+chrM_11423_11976_7:0:0_3:0:0_189/1_GA	chrM	11422	NA	GA	1	GA	1	333
+chrM_11522_12121_5:0:0_2:0:0_13d/1_GG	chrM	11521	NA	GG	1	GG	1	334
+chrM_11080_11521_6:0:0_7:0:0_ff/1_GT	chrM	11521	NA	GT	1	GT	1	335
+chrM_11076_11558_1:0:0_3:0:0_92/1_AT	chrM	11558	NA	AT	1	AT	1	336
+chrM_11197_11674_1:0:0_3:0:0_c1/1_TT	chrM	11674	NA	TT	1	TT	1	337
+chrM_11227_11680_5:0:1_3:0:0_76/1_GT	chrM	11680	NA	GT	1	GT	1	338
+chrM_11759_12257_5:0:0_8:0:0_9b/1_TT	chrM	11758	NA	TT	1	TT	1	339
+chrM_11236_11767_1:0:0_2:0:0_e2/1_TT	chrM	11767	NA	TT	1	TT	1	340
+chrM_11780_12326_4:0:0_7:0:0_10e/1_GG	chrM	11779	NA	GG	1	GG	1	341
+chrM_11789_12313_6:0:0_5:0:0_1f2/1_CG	chrM	11788	NA	CG	1	CG	1	342
+chrM_11815_12366_6:0:0_1:0:0_1be/1_AA	chrM	11814	NA	AA	1	AA	1	343
+chrM_11829_12278_5:0:0_8:0:0_11a/1_CA	chrM	11828	NA	CA	1	CA	1	344
+chrM_11834_12212_5:0:0_4:0:0_131/1_CC	chrM	11833	NA	CC	1	CC	1	345
+chrM_11305_11834_4:0:0_5:0:0_5e/1_TA	chrM	11834	NA	TA	1	TA	1	346
+chrM_11315_11842_2:0:0_8:0:0_126/1_TA	chrM	11842	NA	TA	1	TA	1	347
+chrM_11890_12397_8:0:0_2:0:0_30/1_CC	chrM	11889	NA	CC	1	CC	1	348
+chrM_11898_12351_4:0:0_1:0:0_a9/1_AC	chrM	11897	NA	AC	1	AC	1	349
+chrM_11902_12409_3:0:0_4:0:0_ad/1_AA	chrM	11901	NA	AA	1	AA	1	350
+chrM_11932_12361_4:0:0_6:0:0_44/1_TC	chrM	11931	NA	TC	1	TC	1	351
+chrM_11938_12488_7:0:0_6:0:0_ee/1_GG	chrM	11937	NA	GG	1	GG	1	352
+chrM_11949_12385_7:0:0_5:0:0_118/1_TT	chrM	11948	NA	TT	1	TT	1	353
+chrM_11551_11956_7:0:0_5:0:0_d0/1_CT	chrM	11956	NA	CT	1	CT	1	354
+chrM_11468_11961_6:0:0_4:0:0_c9/1_TA	chrM	11961	NA	TA	1	TA	1	355
+chrM_11495_11994_2:0:0_5:0:0_100/1_AG	chrM	11994	NA	AG	1	AG	1	356
+chrM_11413_12020_5:0:0_2:0:0_4c/1_AG	chrM	12020	NA	AG	1	AG	1	357
+chrM_11598_12060_3:0:0_5:0:0_89/1_AT	chrM	12060	NA	AT	1	AT	1	358
+chrM_11616_12104_4:0:0_7:0:0_6/1_TG	chrM	12104	NA	TG	1	TG	1	359
+chrM_12150_12654_2:0:0_3:0:0_12b/1_CA	chrM	12149	NA	CA	1	CA	1	360
+chrM_12160_12669_8:0:0_2:0:0_1ae/1_CC	chrM	12159	NA	CC	1	CC	1	361
+chrM_11701_12198_5:0:0_2:0:0_96/1_AA	chrM	12198	NA	AA	1	AA	1	362
+chrM_11745_12199_2:0:0_3:0:0_7/1_CA	chrM	12199	NA	CA	1	CA	1	363
+chrM_11818_12273_4:0:0_4:0:0_196/1_TA	chrM	12273	NA	TA	1	TA	1	364
+chrM_11791_12301_5:0:0_6:0:0_17d/1_GA	chrM	12301	NA	GA	1	GA	1	365
+chrM_12330_12776_4:0:0_4:0:0_63/1_AT	chrM	12329	NA	AT	1	AT	1	366
+chrM_12336_12829_5:0:0_5:0:0_f/1_AC	chrM	12335	NA	AC	1	AC	1	367
+chrM_11746_12340_4:0:0_2:0:0_6c/1_GG	chrM	12340	NA	GG	1	GG	1	368
+chrM_12356_12830_4:0:0_5:0:0_104/1_AA	chrM	12355	NA	AA	1	AA	1	369
+chrM_12371_12852_6:0:0_5:0:0_28/1_CT	chrM	12370	NA	CT	1	CT	1	370
+chrM_12405_12863_2:0:0_3:0:0_154/1_AC	chrM	12404	NA	AC	1	AC	1	371
+chrM_12413_12965_5:0:0_6:0:0_174/1_AT	chrM	12412	NA	AT	1	AT	1	372
+chrM_12006_12431_4:0:0_4:0:0_15a/1_AT	chrM	12431	NA	AT	1	AT	1	373
+chrM_11969_12486_5:0:0_4:0:0_2a/1_AC	chrM	12486	NA	AC	1	AC	1	374
+chrM_12495_12966_1:0:0_3:0:0_18a/1_TT	chrM	12494	NA	TT	1	TT	1	375
+chrM_12089_12585_1:0:0_7:0:0_1e4/1_TT	chrM	12585	NA	TT	1	TT	1	376
+chrM_12621_13150_2:0:0_4:0:0_9a/1_CA	chrM	12620	NA	CA	1	CA	1	377
+chrM_12209_12659_3:0:0_3:0:0_1a7/1_GT	chrM	12659	NA	GT	1	GT	1	378
+chrM_12702_13164_4:0:0_3:0:0_88/1_AC	chrM	12701	NA	AC	1	AC	1	379
+chrM_12712_13263_5:0:0_6:0:0_1a3/1_AT	chrM	12711	NA	AT	1	AT	1	380
+chrM_12284_12729_4:0:0_4:0:0_61/1_TA	chrM	12729	NA	TA	1	TA	1	381
+chrM_12259_12743_4:0:0_2:0:0_1a2/1_TT	chrM	12743	NA	TT	1	TT	1	382
+chrM_12260_12784_5:0:0_2:0:0_6d/1_TG	chrM	12784	NA	TG	1	TG	1	383
+chrM_12316_12791_2:0:0_3:0:0_1d6/1_TT	chrM	12791	NA	TT	1	TT	1	384
+chrM_12262_12819_3:0:0_5:0:0_ce/1_AT	chrM	12819	NA	AT	1	AT	1	385
+chrM_12329_12832_4:0:0_4:0:0_1c5/1_TC	chrM	12832	NA	TC	1	TC	1	386
+chrM_12323_12847_3:0:0_3:0:0_163/1_GA	chrM	12847	NA	GA	1	GA	1	387
+chrM_12350_12855_4:0:0_3:0:0_3f/1_AG	chrM	12855	NA	AG	1	AG	1	388
+chrM_12495_12939_2:0:0_4:0:0_117/1_TC	chrM	12939	NA	TC	1	TC	1	389
+chrM_12949_13437_4:0:0_3:0:0_1a0/1_GG	chrM	12948	NA	GG	1	GG	1	390
+chrM_12449_12988_5:0:0_4:0:0_79/1_AA	chrM	12988	NA	AA	1	AA	1	391
+chrM_13032_13560_5:0:0_4:0:0_b1/1_GC	chrM	13031	NA	GC	1	GC	1	392
+chrM_12548_13045_4:0:0_3:0:0_be/1_TA	chrM	13045	NA	TA	1	TA	1	393
+chrM_12698_13119_5:0:0_3:0:0_a/1_GC	chrM	13119	NA	GC	1	GC	1	394
+chrM_12657_13157_3:0:0_2:0:0_a0/1_TG	chrM	13157	NA	TG	1	TG	1	395
+chrM_12629_13160_5:0:0_5:0:0_125/1_GG	chrM	13160	NA	GG	1	GG	1	396
+chrM_12642_13191_2:0:0_5:0:0_1dc/1_AT	chrM	13191	NA	AT	1	AT	1	397
+chrM_13236_13695_2:0:0_9:0:0_1c8/1_TT	chrM	13235	NA	TT	1	TT	1	398
+chrM_13271_13696_3:0:0_5:0:0_188/1_AC	chrM	13270	NA	AC	1	AC	1	399
+chrM_12763_13284_4:0:0_1:0:0_d1/1_AG	chrM	13284	NA	AG	1	AG	1	400
+chrM_13300_13825_6:0:0_3:0:0_ac/1_AA	chrM	13299	NA	AA	2	AA	2	401
+chrM_13300_13872_3:0:0_5:1:0_c2/1_AA	chrM	13299	NA	AA	2	AA	2	401
+chrM_12845_13364_2:0:0_5:0:0_109/1_AG	chrM	13364	NA	AG	1	AG	1	402
+chrM_13502_13977_3:0:0_5:0:0_ae/1_TT	chrM	13501	NA	TT	1	TT	1	403
+chrM_13627_14137_3:0:0_3:0:0_162/1_AC	chrM	13626	NA	AC	1	AC	1	404
+chrM_13055_13651_7:0:0_3:0:0_8c/1_TA	chrM	13651	NA	TA	1	TA	1	405
+chrM_13676_14200_0:1:0_4:0:0_2d/1_CA	chrM	13675	NA	CA	1	CA	1	406
+chrM_13138_13709_5:0:0_7:0:0_72/1_TT	chrM	13709	NA	TT	1	TT	1	407
+chrM_13242_13718_6:0:0_4:0:0_11d/1_AT	chrM	13718	NA	AT	1	AT	1	408
+chrM_13287_13740_4:0:0_5:1:0_1b8/1_GT	chrM	13740	NA	GT	1	GT	1	409
+chrM_13767_14345_8:0:0_5:0:0_f8/1_GC	chrM	13766	NA	GC	1	GC	1	410
+chrM_13776_14348_5:0:0_6:0:0_12f/1_GG	chrM	13775	NA	GG	1	GG	1	411
+chrM_13243_13775_6:0:0_5:1:0_1e/1_TG	chrM	13775	NA	TG	1	TG	1	412
+chrM_13792_14355_3:0:0_5:0:0_d/1_CA	chrM	13791	NA	CA	1	CA	1	413
+chrM_13799_14307_7:0:0_6:0:0_82/1_TT	chrM	13798	NA	TT	1	TT	1	414
+chrM_13306_13843_4:0:0_6:0:0_b/1_AT	chrM	13843	NA	AT	1	AT	1	415
+chrM_13398_13896_7:0:0_5:1:0_1e5/1_GC	chrM	13896	NA	GC	1	GC	1	416
+chrM_13409_13926_6:0:0_2:0:0_13c/1_AG	chrM	13926	NA	AG	1	AG	1	417
+chrM_13997_14575_4:0:0_2:0:0_97/1_AC	chrM	13996	NA	AC	1	AC	1	418
+chrM_13416_13997_2:0:0_2:0:0_d9/1_TA	chrM	13997	NA	TA	1	TA	1	419
+chrM_13540_14114_4:1:0_4:0:0_1b2/1_TC	chrM	14114	NA	TC	1	TC	1	420
+chrM_14120_14578_4:0:0_4:0:0_26/1_CG	chrM	14119	NA	CG	1	CG	1	421
+chrM_14134_14579_3:0:0_5:0:0_da/1_CA	chrM	14133	NA	CA	1	CA	1	422
+chrM_13711_14180_4:0:0_8:0:0_13e/1_GG	chrM	14180	NA	GG	1	GG	1	423
+chrM_14205_14774_5:0:0_5:0:0_105/1_CT	chrM	14204	NA	CT	1	CT	1	424
+chrM_13714_14222_2:0:0_1:0:0_182/1_GA	chrM	14222	NA	GA	1	GA	1	425
+chrM_13745_14261_7:0:0_4:0:0_b3/1_TA	chrM	14261	NA	TA	1	TA	1	426
+chrM_13794_14264_5:0:0_5:0:0_102/1_TA	chrM	14264	NA	TA	1	TA	1	427
+chrM_13867_14400_3:0:0_1:0:0_14f/1_AA	chrM	14400	NA	AA	1	AA	1	428
+chrM_13938_14449_2:0:0_6:0:0_1eb/1_GT	chrM	14449	NA	GT	1	GT	1	429
+chrM_13943_14452_3:0:0_4:0:0_11c/1_GT	chrM	14452	NA	GT	1	GT	1	430
+chrM_14006_14481_5:0:0_3:0:0_df/1_TA	chrM	14481	NA	TA	1	TA	1	431
+chrM_14135_14581_5:0:0_5:0:0_1de/1_AT	chrM	14581	NA	AT	1	AT	1	432
+chrM_14612_15169_2:0:0_5:0:0_e4/1_TT	chrM	14611	NA	TT	1	TT	1	433
+chrM_14668_15109_2:0:0_3:0:0_3d/1_TG	chrM	14667	NA	TG	1	TG	1	434
+chrM_14696_15126_2:0:0_2:0:0_a7/1_AA	chrM	14695	NA	AA	1	AA	1	435
+chrM_14727_15156_4:0:0_4:0:0_178/1_GA	chrM	14726	NA	GA	1	GA	1	436
+chrM_14245_14783_3:0:0_1:0:0_9d/1_GG	chrM	14783	NA	GG	1	GG	1	437
+chrM_14314_14802_5:0:0_3:0:0_191/1_GG	chrM	14802	NA	GG	1	GG	1	438
+chrM_14817_15261_5:0:0_4:0:0_65/1_AT	chrM	14816	NA	AT	1	AT	1	439
+chrM_14863_15320_2:0:0_4:0:0_d8/1_AA	chrM	14862	NA	AA	1	AA	1	440
+chrM_14869_15387_2:0:0_7:0:0_1f/1_AC	chrM	14868	NA	AC	1	AC	1	441
+chrM_14888_15340_3:0:0_3:0:0_112/1_GG	chrM	14887	NA	GG	1	GG	1	442
+chrM_14368_14894_6:0:0_5:0:0_40/1_TC	chrM	14894	NA	TC	1	TC	1	443
+chrM_14410_14941_6:0:0_3:0:0_e5/1_TA	chrM	14941	NA	TA	1	TA	1	444
+chrM_14962_15543_5:0:0_8:0:0_46/1_CA	chrM	14961	NA	CA	1	CA	1	445
+chrM_14456_14987_3:0:0_5:0:0_ab/1_GT	chrM	14987	NA	GT	1	GT	1	446
+chrM_15070_15569_5:0:0_5:0:0_cf/1_CA	chrM	15069	NA	CA	1	CA	1	447
+chrM_15140_15686_2:0:0_5:0:0_185/1_CC	chrM	15139	NA	CC	1	CC	1	448
+chrM_15142_15661_7:0:0_3:0:0_11e/1_CT	chrM	15141	NA	CT	1	CT	1	449
+chrM_15192_15694_5:0:0_2:0:0_f7/1_CA	chrM	15191	NA	CA	1	CA	1	450
+chrM_15211_15685_5:0:0_2:0:0_1d7/1_AC	chrM	15210	NA	AC	1	AC	1	451
+chrM_15225_15786_3:0:0_6:0:0_17e/1_TT	chrM	15224	NA	TT	1	TT	1	452
+chrM_15258_15810_4:0:0_6:0:0_5f/1_TT	chrM	15257	NA	TT	1	TT	1	453
+chrM_14817_15317_5:0:0_2:0:0_59/1_GC	chrM	15317	NA	GC	1	GC	1	454
+chrM_15324_15836_4:0:0_3:0:0_94/1_AC	chrM	15323	NA	AC	1	AC	1	455
+chrM_15365_15880_4:1:0_3:0:0_80/1_CA	chrM	15364	NA	CA	1	CA	1	456
+chrM_15408_15863_2:1:0_3:0:0_1e6/1_AG	chrM	15407	NA	AG	1	AG	1	457
+chrM_15439_15924_0:0:0_4:0:0_172/1_TG	chrM	15438	NA	TG	1	TG	1	458
+chrM_14931_15457_2:0:0_4:1:0_1a1/1_AA	chrM	15457	NA	AA	1	AA	1	459
+chrM_15547_16054_5:0:0_1:0:0_af/1_GA	chrM	15546	NA	GA	1	GA	1	460
+chrM_15134_15560_3:0:0_4:0:0_1c4/1_GG	chrM	15560	NA	GG	1	GG	1	461
+chrM_15575_16118_5:0:0_4:0:0_1aa/1_GC	chrM	15574	NA	GC	1	GC	1	462
+chrM_15052_15629_7:0:0_9:0:0_15f/1_GA	chrM	15629	NA	GA	1	GA	1	463
+chrM_15698_16224_5:0:0_6:0:0_138/1_AC	chrM	15697	NA	AC	1	AC	1	464
+chrM_15247_15721_2:1:0_6:0:0_17/1_TC	chrM	15721	NA	TC	1	TC	1	465
+chrM_15218_15763_2:0:0_2:0:0_171/1_AG	chrM	15763	NA	AG	1	AG	1	466
+chrM_15860_16419_5:0:0_8:0:0_53/1_TA	chrM	15859	NA	TA	1	TA	1	467
+chrM_15864_16329_3:0:0_3:0:0_18b/1_CG	chrM	15863	NA	CG	1	CG	1	468
+chrM_15507_15962_3:0:0_0:0:0_5b/1_GT	chrM	15962	NA	GT	1	GT	1	469
+chrM_15430_15985_5:0:0_3:0:0_2c/1_AT	chrM	15985	NA	AT	1	AT	1	470
+chrM_15706_16238_6:0:0_7:0:0_7b/1_AG	chrM	16238	NA	AG	1	AG	1	471
+chrM_16252_16701_2:1:0_5:0:0_147/1_TA	chrM	16251	NA	TA	1	TA	1	472
+chrM_15753_16280_5:0:0_6:0:0_180/1_TG	chrM	16280	NA	TG	1	TG	1	473
+chrM_15777_16347_2:0:0_6:0:0_16/1_AT	chrM	16347	NA	AT	1	AT	1	474
+chrM_16366_16911_6:1:0_6:1:1_168/1_CC	chrM	16365	NA	CC	1	CC	1	475
+chrM_16370_16833_6:1:0_6:0:1_1bb/1_AC	chrM	16369	NA	AC	1	AC	1	476
+chrM_16402_16876_2:0:0_1:0:1_98/1_CA	chrM	16401	NA	CA	1	CA	1	477
+chrM_16426_16953_3:0:0_2:2:0_60/1_AA	chrM	16425	NA	AA	1	AA	1	478
+chrM_15986_16442_3:0:0_7:0:0_15d/1_TG	chrM	16442	NA	TG	1	TG	1	479
+chrM_16030_16460_6:0:0_4:1:0_20/1_AT	chrM	16460	NA	AT	1	AT	1	480
+chrM_15909_16463_3:0:0_7:1:0_106/1_GT	chrM	16463	NA	GT	1	GT	1	481
+chrM_15984_16500_4:0:0_1:0:0_6b/1_GT	chrM	16500	NA	GT	1	GT	1	482
+chrM_16070_16513_3:0:0_7:1:0_1e7/1_TA	chrM	16513	NA	TA	1	TA	1	483
+chrM_16064_16572_4:0:0_6:1:0_f1/1_TG	chrM	16572	NA	TG	1	TG	1	484
+chrM_16245_16740_4:1:0_2:0:1_127/1_TA	chrM	16742	NA	TA	1	TA	1	485
+chrM_16315_16834_5:0:0_3:0:1_18/1_TT	chrM	16834	NA	TT	1	TT	1	486
+chrM_16229_16843_2:1:0_2:0:1_c4/1_GC	chrM	16843	NA	GC	1	GC	1	487
+chrM_16420_16905_5:0:0_2:0:1_159/1_GT	chrM	16905	NA	GT	2	GT	2	488
+chrM_16420_16905_5:0:0_2:0:1_159/1_GT	chrM	16905	NA	GT	2	GT	2	488
+chrM_16419_16923_3:0:0_3:0:1_183/1_CG	chrM	16923	NA	CG	1	CG	1	489
+chrM_16585_16993_5:0:1_5:1:0_1b9/1_GA	chrM	16993	NA	GA	1	GA	1	490
Binary file test-data/group_out4.bam has changed
Binary file test-data/group_out5.bam has changed
Binary file test-data/group_out6.bam has changed
Binary file test-data/out_R1.fastq.gz has changed
Binary file test-data/out_R2.fastq.gz has changed
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/out_SE.fastq	Wed Jan 10 19:09:42 2018 -0500
@@ -0,0 +1,288 @@
+@HISEQ:105:C2UE1ACXX:3:1101:11160:2245_TTA 1:N:0:CAGATC
+AAAAGTAGTTAATATATTAGATTTGTTTGATAGTGGTAGTATATATTTTTTATTTTAGTATTTAGGAGGTAGAGGTAGATGAATTTTTGAGTTTAAAG
++
+BBBFFFFBFFFFFIIIIIIBIIIIBIFFBFIIBBFFFFFFFFIIIIIIIIIIIIIIIBFIIIIIB7BBBFBBFFFF77<F7BFFFFFF7B7BBFFFF7
+@HISEQ:105:C2UE1ACXX:3:1101:19467:2281_TTT 1:N:0:CATATC
+TTGGTTAGGGTGAGATGTATAGTTTGGATTTTAGTGATTTTTGTAAAGGGGGAAAAGAATGGAGTTTTGGGTGTAGTGAGAGGTTATAGGAGTAGGGA
++
+<B<<FFFBBFFFFFFFFFIFIBFFI<<BFFFIIBBBFFIIIIBFFIIBBF7BBFFFBFFF77BBFBFF777BBBBBB<<<B<7<7BBF77<7<70000
+@HISEQ:105:C2UE1ACXX:3:1101:7009:2740_TAG 1:N:0:CAGATC
+AAGTTTTGTTTTTTATTTGGAGGTTATGGAATGTTAAGTAAGGTTTTTTTGGGTTTTGTTATTTATTTGATAATTGTGATTGTAATGTTAATAAGGGA
++
+BBBFFFFFFFFFFIIIIIB<FFFFFFIBBFIIFFFIIFIIIFFIFFIIII<0<BBFFBFFFFFFFFFF<BBFFFF<BBBBF7BFFF<BBFFFFF<00<
+@HISEQ:105:C2UE1ACXX:3:1101:19067:2707_TTT 1:N:0:CAGATC
+GTTTTTTTATTTGATATTTTAAAGGTTTTTTTTTTTTTTTTAGAAAATTTTTTTTAGTAAGATAGATTTTAAAGGGTTTGTTTTTTTTTTTTTTTTTT
++
+BBBFFFFFFFFFBBFFFFIIIFIFFIFFIIIIIIIFFFFFB7'0<B0<BBFF'7<0'0<<''0<'0'0<BBB<<B'7'0'0'0<BFFFFFFFFFFFFF
+@HISEQ:105:C2UE1ACXX:3:1101:4999:3182_AGG 1:N:0:CAGATC
+GTTTTATGAGGATTTTAGGGGAGTGATTGTTTAAAGTTTATAAGATTTATGATTTATATATAGTTAGAATAGTATGTGTTAAATAAATATAAAGGGAG
++
+<BBFFFFBFFFFFFIIFBBBFFFIIFFIBFFIFIIBFFIIIIIFFIIIIIBFFIIIIIIIIIBFFIBFIII<FFFBFBFFFFFFFFFFFFFFF<07<7
+@HISEQ:105:C2UE1ACXX:3:1101:2300:3263_GTT 1:N:0:TAGATC
+TTATTTTTAATAAAATTTTTATTATTTAATTTATTAGTTAATATTTAGGAGTTTTATGTTGTGGTAAAATTTTGTTAGAGAGATAGAGAAAGTATTTA
++
+BBBFFFFFFFFBFFIIIIIIBIBBFFI0<FFFBFF70B00<'0<FFFBBBBFF''0B0BB000'7BBFFFFII'<BF0<'<''07'70BBF'7BBBFB
+@HISEQ:105:C2UE1ACXX:3:1101:5605:3427_AGA 1:N:0:CAGATC
+AGATGAGAGGTATAGGATGTGGGGAGTTTTAGTAAGATTTATAGATAAGAAGTGGTTCGGTTATAGGATTTGTTTTAGATTTTTAGATTTTTTTGTGT
++
+BBBFBFFFFFFFFFFBFFFFIIIIIIIBFFFBFFIBFFIIIIIFFIIIFFIBF7BFBFBBFFIFF<<FFFFBBFFBF0<BFFFFF7<BFFFFFF0B7B
+@HISEQ:105:C2UE1ACXX:3:1101:8129:3589_GAT 1:N:0:CAGATC
+TTTTAGTTTTTAGTTAGGATTATACGTTTATTGTGATAAAAGAGTTTTTTGATTTATCGGGTTATGTTAGGGTTTATTGATATTAGGGAATTTGAAGA
++
+BBBFFBFFFFFFBFFFBBFIIFIIFBFFFFII<BBFFFIIIBFFIFIIIIBBFFIIII<7<FBFF<FBF<7<BBFFFF7<BFFFF700<BBFF0<B'<
+@HISEQ:105:C2UE1ACXX:3:1101:14304:3866_TAA 1:N:0:CAGATC
+GTGTTTATATAGGGGATTTTTGAGTTTGATAGGTTGTTTTTGTAGAGGGTAGAATTTTGTGGAAATGTTGGTATTGGTTAAGGGGTTTTAGTGAAGAA
++
+<BBFFFFFFFFFFFFFFFFII<BBFFFBFFIFBFFBFFFII7FFFFFFFIF<BFIIII<F<BBFIFBFB7<BFFFBBBBBF777<B7BBF7B<BB7BB
+@HISEQ:105:C2UE1ACXX:3:1101:12720:4398_AGT 1:N:0:CAGATC
+GTATGTGTGTGTGTGTGTGTATTTAATTGAAGTTGGGTTTGGTGATATATATGTTTAATTTTAGTATTTTAGTGGTAGAGGTAGGTTAATTTTTGTTG
++
+<BBFBFFFFFFFFIFIFIFFFFFIIFII0BFBFFB7BFFF<BFFFFIIIIIIBFIIIIFIIII<FIFIIIFBFBBFB7B<<BB7<B<BFFFFFF0<B0
+@HISEQ:105:C2UE1ACXX:3:1101:14945:4439_ATT 1:N:0:CAGATC
+AGTGTTGAGTGGAGTATTAGAGAAGAGAAATAAGATAATAAAGTAATAGTTGTGATTAGGAGGTTTTTATAAGTTGATGGTTTATGTTAAGTAAGTTT
++
+BBBBFFBFFFFFFFFFFIIBIFFIFFBFFIIIIBFFIIIIIIBFFIIIBFFBFFFIIIFBFFBFBFFIIIIIFFFBBF77BBFFF7BBFF<BBF<<BF
+@HISEQ:105:C2UE1ACXX:3:1101:8616:4508_AGG 1:N:0:CAGATC
+AGAAATTTTGGGGGTGTAGGAGTGGTAGGATAGGAGTGTTGTTTTGTAATAGTTTTTTTTGAGGTTTAATAGGTAGGGTAGTTATTTTTAGTATTGTA
++
+BBBFFFFFFBBBFFFIIFFFIFFFIIFFIIFIFBFFFIIF<FBFI<FFIIIBFFFFFFFF077<B<BBBFF<<BBBBBB<7B<BFFFFFF0BBFF0<B
+@HISEQ:105:C2UE1ACXX:3:1101:18975:4834_GAG 1:N:0:CAGATC
+TATAAATGGTAATTTTGTAATTTAAAGATTTAAAAGTAATTATTGGTAATAGTTATTTGTGGGAGGTTGAGGTAGGGGGATTTTTGTAGAGATCGGAA
++
+BBBFFFFBBFFFFIIIBFIIIIIFIIFFFFIFIIIBFIIIIIIIB<FFIFIBFFFIIIBFFBFFFII<<BFBFFFFIFF<BBBFF0BB7<<<BF707B
+@HISEQ:105:C2UE1ACXX:3:1101:4984:5374_TTG 1:N:0:CAGATC
+TGTATCGAGGTTTGAATGAGAGTGGTATTTTTGTTATTTGTTAGTTAATGGTTTTGAGTATTAGTTTGGAAAATGATAATAAGTATTAGTTGAGGTGT
++
+B<BFFFBFFFFBF<FFFBFFFFFFFIFFFIII<BFFIIIBFFFFFFIIIBBFFFIBBBFFFIIBFFF<<BFFFFBBFFFFFF<BBFFF<BB7<7<BBB
+@HISEQ:105:C2UE1ACXX:3:1101:12336:6058_TTT 1:N:0:CAGATC
+ATTTAGATGATGGTTTTTTTATTTGATATTTTAAAGGTTTTTTTTTTTTTTTTTTAAAAAATTTTTTTTATAAAAATAAATTTTAAAGGGTTTTTTTT
++
+BBBFFBFFBFFBBFFFIIIIIIIIBFFIIIIIIIIBFFFFIIIIIFFFFFFFFF700<BF0BBFFF0<70''00'0'000'<<'<B<00'0'00007<
+@HISEQ:105:C2UE1ACXX:3:1101:5999:6265_ATG 1:N:0:CAGATC
+TATATTGTTATATATTTGTGTTTTTTTTGAATTTATTTTTTGTTTATTAGTTTTTGTGGTTGATAGGGTTTTTGGATATTGGGTTGTTTTTATATATA
++
+BBBFFFBFFFFFFIIIIBIFIFIIIIII<BFFIIFIIIIII7BFFFIIFBFFIII<B<BFB<BBF<BBBBBFF00<BFFF700B<7<BFFFFFFFFFF
+@HISEQ:105:C2UE1ACXX:3:1101:5836:6568_TTT 1:N:0:CAGATC
+ATTTGGATGTTAAGGATTGGTTTTTTTTTTGATTTAGGGTATTGTTAGATAGTATAGATTTTACGGAGTTTTGTATTTGAAAGGTTGTTGGTATGTTT
++
+BBBFBBFFFFFFFFFFFFBFIFIIIIIIII'0<FFB<BBFFBF7BFF7BFF<BBFF<BFFFFFF<<BBFBBF7BBBFF'<BB0'<B0<B0'7<B<BFF
+@HISEQ:105:C2UE1ACXX:3:1101:12432:6996_GAT 1:N:0:CAGATC
+AAGTTATTTATGAGATTAGTTTATAGTAAATTAAAAAGGGTCTTAGTATTTTGATTATGAAATTAATTATTTTAGTAATATTTGAAGTTGTAATTTTA
++
+BBBFFFFFFFFBFBFFIIBIIIIIIBFIIIIIIIIIIFBFFFFFIFFIIIIIBFFIIIBFIIIIIIIIIIIIIIBFFIIIIII7BF<BF<BFFFFFFF
+@HISEQ:105:C2UE1ACXX:3:1101:2440:7931_TGA 1:N:0:CAGATC
+ATTTGAAAAGTGTTATAGATATTAGAAAGGTATGGAGATGAGAGGTATAGGATGTGGGGAGTTTTAGTAAGATTTATAAGATCGGAAGAGCACACGTC
++
+BBBFBFFFFFFFFFIIIBFFIIIIBFFIBBFFIBBFFIFFFFFFFIFFIBBFFFFFFFIFIIBFFIBFFFBFFFFFFFF7BBF70<B0<7<BBBF0<B
+@HISEQ:105:C2UE1ACXX:3:1101:6407:7896_ATC 1:N:0:CAGATC
+TTTATAGGTTTGGGTTGAGGATATGTATTGGGAGTTATTAGAGGGTTATAGTTAGGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATC
++
+BBBFFFBBFFF<BBFFBFFFFFIIBFFIIBBBFFFFFIFFBFFFFIBFFIBFFIBBFBFFFFI<BBF<F<BFFFFBFBF<BBFFFFB<BBFFFB0<BF
+@HISEQ:105:C2UE1ACXX:3:1101:19497:7952_ACC 1:N:0:CAGATC
+ATACGTAGATGTTTAAAAGTTTAAATAAATGTATTTAGAGGTTTATGAGGTTTATGGTTAAGTGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC
++
+BBBFBFFBFFBFFFIIIIBIIIIIIIIIII<BFFIIFBFBFFFFFIBFBBFFFIIB7FFFIBFBBBFFFFFBBBFB<0<BFFF7<BB7BBBFFFF<BB
+@HISEQ:105:C2UE1ACXX:3:1101:3853:8232_TCC 1:N:0:CAGATC
+GGTTAGGTTGTTGTTGTATTTAGAGGTTTTTAGGAGTTGTTTTTGAGTTGTTGTTTATTTAGTTTGTCGGTAGAGATCGGAAGAGCACACGTCTGAAC
++
+<<BBFBFFFBFFBFFBFFFIIF<FBFIFFIIFBBFFIFBFFFIIBFBFFBFFFFFIFIIII<FFIBFI<<BF<BB<BF70<B0<7BBFFF0BBB7BBF
+@HISEQ:105:C2UE1ACXX:3:1101:7150:8384_TTA 1:N:0:CAGATC
+GAGTAGTAGTTTTTGTGTAGGTTTTGGTTTGTGTGGTATTAGTAGGTAGTAGTGTGTTAGTTGGAGCGATAGTAGTAAGTTATGGTTATTGTTTATTA
++
+BBBFFFFFFFFFFIBFBFFFFFFFFB<FFF<BBFFFFFFIFBFIFFFIIIIFFFIFIFF<FF7<BFFFFFFBBFBBBF<BBFF77<BBFF7BBFFFFF
+@HISEQ:105:C2UE1ACXX:3:1101:11512:8610_TGT 1:N:0:CAGATC
+TGTGTTTTGAAGTTGGCGGAGTTCGCGTTTTTAGGAATTTTGTTTTGGAACGGTTTGGTCGGGGTTTTCGTTTTTTTTTGTAGAAGTGTAGGAGATGT
++
+B<BFFFFF<FFBFFBBFFFFBFFFBFFFFFIII<7BFFIII7BFFI00<BFBBBBB77<B0<<<B7BBB7BFFFFFFFF'7<000'<'0B'0<<BB0<
+@HISEQ:105:C2UE1ACXX:3:1101:18629:8512_TAT 1:N:0:CAGATC
+TGAATTAATGAATTTTAAAAAAAAAGAAAGTAAGTTGTTTTTAGGATTGATATTTAGAGTTAATTTTTTGAGTTTTTTTGTTTGTTTAGGTTTTGGTT
++
+B<BFFFFFFBFFFIIIIIIIIIIII<FFIBFFFBFF7FFFIIF<<BBF7BFFIIIF7B<BBFFFFFFFF0<7BBBFFFF'0BB'0<B<'0<<B'''7B
+@HISEQ:105:C2UE1ACXX:3:1101:5146:8981_AGT 1:N:0:CAGATC
+TTTAGGTTTTAGTTGTTGTTGTTTGTTTTGTATTAGTGTAGTTATAATTTTTTTGTTGATTTTGTTGTTTTTAGAAGGTTTGGGGTGAGTTTAGTATG
++
+BBBFBBFFFFFBFF<FF<BFBFFI0BFFI0BFFFF<BBFFFFFFIIIIIIIIII7BF7BFIII<BF7BFFFFF0<<7'<<B00'0<<77BBBF'<BB0
+@HISEQ:105:C2UE1ACXX:3:1101:3344:9104_ATT 1:N:0:CAGATC
+TAGATGTTGAGTGTTTGTTTTGTGTTAGGTATTAGGTGGGATTAGGTGGTTATTATCGAGTTTTTAGGAGAGAGTATAGTTGTAAGCGGACGATAATA
++
+BB<FFFFFBFBFFFFFBFFIIBFBFFFBBFFFFFBBFFFIIFFFFBFFFIFFIIIII<FBFBFIIF<<BBFFFFFBBF<BB7<BF0<007B7<BFFFF
+@HISEQ:105:C2UE1ACXX:3:1101:2097:9536_GTT 1:N:0:CAGATC
+TTGGATTTTTTGGTTTGATAAGGAGTTTTGTAGGTTAAAGAATGTTTTTTAAGGGATTTTTGTTATGTGATTAGTTAATGGGATTTTATTTAAGTTTG
++
+BB<BFFFFFFFBBFFFBFFIIFBFFIFFIBFIFFFFFIIBFFIFFFIIIIII<<BFFFIFF<FFFFBFBFFFFBFBFFF700<BFFFFFFFFF<BBF<
+@HISEQ:105:C2UE1ACXX:3:1101:16351:9944_TTG 1:N:0:CAGATC
+TTGGACGGGATGGTTGTGGTAAAGTTTTAATAGTTTTATTGGGGGATGGTATAGAAGTAGTTTAGGTTGTATTGTGGGATTTATTTTTATTGTTTGTT
++
+<B<<FFFFFFFFFIFBFFFFFIIBFFIIIIIIBFFFIFFI70BFFFFFFFFFIBFFBFFBFFFFBBBB<BFFF<B<BBBBBBFFFFFFFFF0<BF0BB
+@HISEQ:105:C2UE1ACXX:3:1101:17628:10309_GTC 1:N:0:CAGATC
+TGTATGTTTAGATTTGTTTTGAATTTGCGTTTTTGGTTGTTGATTATTTTTTATTATTTTTTGTTTTTTCGTGTGTTCGGGAGATCGGAAGAGCACAC
++
+B<BFFBFFFFBFFIIBFFFI<BFFFI<F<FFFII<0BF<FF0BFFFIIIIIIIIIIIIIIIF7BFFFFFF7B7B<BBB7''707BF007B0<07<BBF
+@HISEQ:105:C2UE1ACXX:3:1101:1440:10515_TGT 1:N:0:CAGATC
+AGAGTGTTTTTAGTGTGAGGAGTTGGATGTTTTAGATATTTATTTTTATATTGTTTATTTAGGTTATGATTTATGTTTTATTTTAGTTTAATTTTTTG
++
+BBBFFFFFFFFFBFBFFFFIIIIFBBFFFFFFIF<BFFIIIFIIIIIFFFFIBFFIFIIIFBBFFFF<BFFIIIBFFFIIIIFFF7BFFFFFFFFFF7
+@HISEQ:105:C2UE1ACXX:3:1101:11787:10735_ATT 1:N:0:CAGATC
+AGAATAGGGAAGTGTATAGAAGTGATATATAGTTTAGATTTGAGTTTTTATTTTGTTTTTTATATTTTTAGATATAAGTAGTTATTAGATTTTTTTGA
++
+B<BFFFBBBFFFFFIFFIBFIBFFIFIIIIIBFFIIBFFIIBFFFFFIIIIIIIBFFIIIIIIIIIIIII<FFIIIF<FF<BFFFFF0BFFFFFFF0<
+@HISEQ:105:C2UE1ACXX:3:1101:10231:11124_TGT 1:N:0:CAGATC
+TTGTTTTAGTTATTTTTTGGTTTTTTAGATTTTATGTTTTTAGTTTTTATAATTTTTATATTGTATTTGTTTATTAAGTGTGAAATGTTTTTGTAGGA
++
+BB<FFFFFBFFFFIIIIIBBFFFFIIF<FFFIIFF<FFIIIFBFFFIIIIFFIIIIIIIIII<FFFFF<FFFFFFFF<B<B<BFFF<BBFFF0<B70<
+@HISEQ:105:C2UE1ACXX:3:1101:9833:11396_CAC 1:N:0:CAGATC
+GTTTTTATTTTTGGGATTAAGGTAGTTGTTTTTTGATTTTTGTGTGTTATAGTAAGAATGATATAGGAGATCGGAAGAGCACACGTCTGAACTCCAGT
++
+<BBFFFFFFFFFBBBFFFIIFFFFFFIBFFIIII<BFFIII7FBFBFFIII<FFFBFIIBFFIIF<7BBBFF<7B<0<7<BFFF7BBF7BBFFFFF<B
+@HISEQ:105:C2UE1ACXX:3:1101:13437:11566_TTT 1:N:0:CAGATC
+TTATTATTGTGGATTTTTTTAATAGTTTGAGAATGAGTTGATTTAGGGTTTTTATTTTATTTTTAAAATTAGGAGTATAATGGGTTTAAGATATAGTT
++
+BBBFFFFFBFFFFFFIIIIIIIIIBFFF<BBFFF<FBFFBFFFIFB7BFBFFIIIIIIIIIIIIIFIFFFF<7BBBBBFFF700<<BFF<BBFFF<<B
+@HISEQ:105:C2UE1ACXX:3:1101:10809:14594_AGT 1:N:0:CAGATC
+GTATTAGAGAAGAGAAATAAGATAATAAAGTAATAGTTGTGATTAGGAGGTTTTTTATAAGTTGATGGTTTATGTTAAGTAAGTTTATTAAGAAGTAT
++
+<BBFFFBFBFFFFFFFIIIIBFFIIIIIIBFFFIIBFF0BBFFFIBBFFFFFFFIIIIIIBFB7BFBBFFFII<BBFF<BBF<BBFFFFFF7<B<BBF
+@HISEQ:105:C2UE1ACXX:3:1101:7778:14963_TAT 1:N:0:CAGATC
+AGTGGATGATAATAATTTAGGTGATTTATAGTGAGATAAGAGGTATATGAATTTTAGTATTTATTTGTTTTTTTATTTTGGGTTATTTGAGGGTGTTT
++
+BBBFBFFBFFFFFIIIIIIBBBFFFIFFIIBFBFBFFFIBFFFFFFIIBFIIIIIIBFIIIIIIIIBFIIIIIIFFFFF707<<BFFF7<''077<BB
+@HISEQ:105:C2UE1ACXX:3:1101:2575:15554_AAA 1:N:0:CAGATC
+TTTGGGTTGGTTTTTTTTTTTTTTAAAAAGGTTTTAGGGTTTTGGTTTTTTTTATAATTTTATTAAAATATTAAATATTTATAAAATTTTTATTTTAA
++
+BBBBBBFFBBFFFIIIIIIIIIIB7'0'00'7<'00<B0<<BB7'7BBFFFFB'0<'<BFB'<<BB'00'0<'0'00<'000'0B0<BFF<'<BF<BF
+@HISEQ:105:C2UE1ACXX:3:1101:20234:16099_GGT 1:N:0:CATATC
+TAAGAGGAAAGAATGGAGAAGAAGATTGGTTTTAGGTATATGATGGAATAGGGTAAAAATGATAGTTGAGGTTAATGGTTTAATTTTTGGGAATTATA
++
+BBBBFFFFFFFFFIBBFFFFFFIBFFFB<BBFFFBBFFFFIBFFBBFFFFBBBFFFIIII<BFIBFF<BBFFFFFB77BBFFFFFFFF000<BBFFFF
+@HISEQ:105:C2UE1ACXX:3:1101:20842:16102_TTA 1:N:0:CAGATC
+TTGGGATTGTTTTTGTTTGAATTATTTTTTTGTATTATATTTTAATGTGTTGTTTATTTTTAGAATATGGAATTTGTAGGAAATTTTTAAAATTTATT
++
+<B<BBFFFFFFFFIBFFIBFFIIIIIIIIII7BFFIIIIIIIIIIIFFFFFBFFIIIIIIII<BFFFF<7BBFFF<BB00<BBBFFFFFFFFFFFFFF
+@HISEQ:105:C2UE1ACXX:3:1101:21194:16434_TTT 1:N:0:CAAATC
+TATAGGTTTTTATTTATGTAGTTTTTTGTATTTGTTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
++
+BBBF<<BBFFFFFIIFI<BFBFFFIII7BFFBF0BFFFI7BFFFIIFIFFFFFBFBBFFFBBBFFFBFFFFFFFFFFFFFFBFFB<<7BBFFFFFFFF
+@HISEQ:105:C2UE1ACXX:3:1101:7164:16848_ATA 1:N:0:CAGATC
+TTTTTGAGTTTGATAGGTTGTTTTTGTAGAGGGTAGAATTTTGTGGAAACGTTGGTATTGGTTAAGGGGTTTTAGTGAAGAATAAATTTTTATGTTGT
++
+BBBFFBBBFFFBFFIBBFIBFFFII<BFBFFFIIFBFFFFIIBFB0BFFIBFF<<FFFI77BFFI<<<FF<BBF<B<<B7BBFFFFFFFFFFF'<B7B
+@HISEQ:105:C2UE1ACXX:3:1101:12454:17532_TTT 1:N:0:CAGATC
+TTGGTGTAGAGGTTTGTTAAGTGTGGTATGGTTGAGAATATTCGTGTTTTATTGGTTAAATGTGATTTTTGTATTAAGTTGAGAGTTAGGGGTTTTGT
++
+<B<<BFFFBFFFFFF<FFFFBFFIFFIFFBBFF<BBFFFFIIIFFFFFFIFIIB7FFFFII<FBFFIIII<FFFFFF<BB7B7B<B<B7''7<<BF<B
+@HISEQ:105:C2UE1ACXX:3:1101:2584:17964_TTT 1:N:0:CAGATC
+TAGAGGGTTTTGTAGAGTATTTGAGTATTTGTTATTATTGTTGTATTTATAGTATTAATTTTTTTAGGAGTAAAGTAATTTTATTAAGTTTTAATAGA
++
+<B<FFFFFFFFBFFBFFFFFFIBFBFFFIIBFFFFIIIIBFFBFIIIIIIIBFIIIIIIIIIIIII<7BBFFFFFFBFFFFFFFFFF<BBFFFFFF7<
+@HISEQ:105:C2UE1ACXX:3:1101:9624:17911_AAT 1:N:0:CAGATC
+ATTTTGATGTTATTATTTATTTATAAAGTAAGAATAAGGTTATTGGTTTTGTTGTTTTTTAGAGTTGTAAGGAGGATTTTATATAGTAAAAGATGTAG
++
+BBBFFBFFBFFFFIIIIIIIIIIIIIIFFFFFFIIII<BFFFFIBBFFFIBFIBFFIIIIFBFBFB<FFIBBB<<BBFFFFFFFF7<BFFF<BB7BB7
+@HISEQ:105:C2UE1ACXX:3:1101:19795:18124_GGA 1:N:0:CAGATC
+TTTATATTAATTTTTGGGTATTATTTTAGTGTGTTTAAGATGATATTTTAAGAGAAATTTGAATTGAAGAGATTAGTTTTTTATAAATGGCGTATGAA
++
+BBBFFFFFFFFFFII<<<FFFFIIIIII<FBFFFFFIIBFFBBFIIIIIIIBFBFFIIIIBFFFI<BFBFBFFFIFFIIIFFFFFFFF7'70<BB7<B
+@HISEQ:105:C2UE1ACXX:3:1101:2293:18893_TGA 1:N:0:CAGATC
+TGGATGCATGAAAAGTGTATTTAATTGGTGAGTTATATTTTTTGTCTTTTGGTTTTCGTTTTTAGATATTTATTTATTAGTTTTTATTGTTTTTAGTT
++
+<<<BFBFFFBFFFIBFBFFFIIFIIIB<BBFBFFFFFIIIIIIBFFIIII70BFFII7BFIIII7BFFFFFFFFFFFFF7BBFFFFFF<BBFFFF7BB
+@HISEQ:105:C2UE1ACXX:3:1101:12741:20220_TGT 1:N:0:CAGATC
+ATTTTTTTGGAAGAGAAGGAATTTATTTAGGGGAAATATATGTGTTTATATAGGGGATTTTTGAGTTTGATAGGTTGTTTTTGTAGAGGGTAGAATTT
++
+BBBFFFFFB<FFBFFFFFFFFIIIIIIIIBBFFFFFIIIIIBFFFFIIIIII<77BBBFFFF7BBFBF<BBF<<B<<BBFFF7BF7B<'0<<0<BFFF
+@HISEQ:105:C2UE1ACXX:3:1101:18680:20134_TTT 1:N:0:CAGATC
+TGTTTGGAAGTATGGTGTTTTTTAAGAGGTTTTTGGTTATTTAGTTTGAAATGATTTAGATTATTTTGTTTTTTTGGTTGGGGTAGGTTTTGGAAATT
++
+B<BFFBBFFFFFFFFFFIFFIIIIIBFFFIBFFIB7BFFIIIIFFFI<BFIIBFFIIIBFFIIIIIIBFFFFFFF<7BB70077777BBBF7'<BBFF
+@HISEQ:105:C2UE1ACXX:3:1101:19451:20037_TAT 1:N:0:CATATC
+GTTGGTATATTGAGTTTACGTTGCGTTTGTATTATGGAATTTTTTTTGGTGTAGTTTTTGAAGGTGTATTGAGGTTTGAATGAGAGAGGTATTTTTGT
++
+0BB<BBFFBFF0BBFFIIIBFF<B0BFF0BFFFIIB<BFFFIIIIII<7B<BBBFFFFF7<B<BBBBBBF0<70<7<'<BB'<7<'707<<BBFFB07
+@HISEQ:105:C2UE1ACXX:3:1101:14848:20360_GAT 1:N:0:CAGATC
+TCTTTATTAATATATAAAAAAAGATATTAGGGAGGATCGAGGGTGTTTTTGTTATGTTTGTTTTATAGAGGAGTATATAGATTGAGGTATAGATACAG
++
+BBBFFFFFFFFFFIIIIIIIIIBFFIIIIBBBFFFFFFBFFFFIIIFFII<BFFFBFFFBFFFFFFF<B<<BBBBBFFF7<BF7<00<<BF0<BFFF0
+@HISEQ:105:C2UE1ACXX:3:1101:12295:20565_TTT 1:N:0:CAGATC
+ATAAGATAATAAAGTAATAGTTGTGATTAGGAGGTTTTTTATAAGTTGATGGTTTATGTTAAGTAAGTTTATTAAGAAGTATAGTATTATATATAGTA
++
+BBBFFFFFFFFFFFFIIIIFII<FBFFIFBBFFFIFFFIIFIIIFFF7BFBBFFFIIBFFIIFFFIFIFFIIIFI<<B7<BFF07<BFFFFFFFF77<
+@HISEQ:105:C2UE1ACXX:3:1101:11915:21129_GTA 1:N:0:CAGATC
+TTTGTTAGTTTTATATTTTTAAGTTTTTTTTTAGGTTCGTTTTAGAAATAGTATGTGTTAAATAGTGAAGTAATTTTTGGTTTTATAATGAGGAGTGG
++
+BBBBFFFFFFFFFIIIIIIIIIFIIIIIIIIIIB7FFF<FFFII<FFIIIBFIFBFFFFFFFFF<BBBFBBFFFFFFF00<BBFFFFFF7<00<0<<<
+@HISEQ:105:C2UE1ACXX:3:1101:12442:21376_GTT 1:N:0:CAGATC
+GTGTATATATTAATTATATTTTGATATGTTTGTATAGGACGGTTATTGGAATAAAATATAAAAAAGTAAGTAAGTAAATAAAAAAATATAAGGTGAAA
++
+BBBFFFFFFFFFFIIIIIIIIIBFIII<FFI<FFII<BFFBBFFFII77FFFFIIIIIIIIIIIIBFFFBFFFBFBBFFFFFFFFFFFFFF7077<BB
+@HISEQ:105:C2UE1ACXX:3:1101:10225:21919_GTG 1:N:0:CAGATC
+AGTGAAGAGGGAATGAAAAGTTTTTGATTTAATTTATATAATTAAAAAAGTTGGTAGTTATAGTAGTTTAATTTTTTATTATTAAGTTTGTGTGTGGT
++
+BBBFFFFFFFFFFFBFFIIFIFFII<BFFFIFFIIFIIIIIIIIIIIIIFFB77BFBFFFII<FFBFFFIFIFFFFFFFFFFFFF7<BF7B<B<B<7<
+@HISEQ:105:C2UE1ACXX:3:1101:12240:21986_ATC 1:N:0:CAGATC
+ATTGTTATTAATATAGGGGGTAGTTTTGGTTTGGGATTGAGGGAGGAATTATAGGAGAATTATTTTTTGTGTAGGAGGGAGAGTTTGAAATTAGGAAG
++
+BBBBFFFFFFFFFIIFBFFIIFFIFIIBBFFF77BFFF7B<BFFBFFFFIIFF<<B<BFFFFFFFFFF7BBBFB<<<<<<<BBBBB0BBFFFF00<B7
+@HISEQ:105:C2UE1ACXX:3:1101:2199:22213_GTT 1:N:0:CAGATC
+TTAAAGTTATTATTGGAATTTTAAGTTAGGTTATATGTAGGTTATGTATAGGTTTTTATTGTGGGATGAATGGTATTTGGAGCTTGTTTAAAAGTTAG
++
+BBBFFFFFFFFFFIBBFFIIIIFIBFFIBBFFFIFIFIIFFIFFIBFIIIFBFFFIIIIIBFBBFFFBFFF<<FFFFI70<7BFF<BFFFFFFBBBF7
+@HISEQ:105:C2UE1ACXX:3:1101:13060:22287_TGT 1:N:0:CAGATC
+TAGTGAAGAATAAATTTTTATGTTGTATATTATTTTTTTTTAGTTCGTATATATCGGTATACGTGTTAGGATTTATAAAGATAGTTATTATTTTTTGT
++
+BBBFFFFFFFFFFIIIIIIIIBFIFFIIIIIIIIIIIIIIII<FFIBFFIIIIIIB<BFFFFBBBBBF<<BBFFFBFFF<BBF7<BFFFFFFFFFF7B
+@HISEQ:105:C2UE1ACXX:3:1101:7272:22581_TTA 1:N:0:CAGATC
+TTAATGATATTAAGAATTTTTTAAAGAATTTTATTTTTTTTAGGAATAGAAGGAGGAGGAGTATTTTGATCGATTTTTTAGGTTTTTTATAGGTGGAG
++
+BBBFFBFFFFFFF<BFIIIIIIFIIBFFFFIIFIIIIIIIII<<FFFIBFIBBFFBFFFFFFBBFFF<BBF<BBFFFFFF00<BBFFFFFF000<777
+@HISEQ:105:C2UE1ACXX:3:1101:10060:23020_TTT 1:N:0:CAGATC
+TTTTAGATTATTTAAGAAGGTATTAGGTTTTTAAGAGGAAAGGGTAGTCTTATAGTTTTGAGTATTTTTTTTAAAAGGAAGTAAGGATGGTGTTTTTA
++
+BBBFFBFFFFFFFIIBFIFFFFFIBBBFFFFIIIBFFFIFIBFFIFFFFIIFFFBFFII7B<FFFIIIIIFFFFBB77<B<BBF<7BB<<BBBBBFFF
+@HISEQ:105:C2UE1ACXX:3:1101:14440:23104_TTT 1:N:0:CAGATC
+TTTAAATTTAAGTTAAGGTTTGGGGAGTTGATTTTTGTTTTGTGGGTTGTTTTTTTTGTAGGAGTTGGTTTTTAGAGGTTTTTAGGAATTTTTGGTGT
++
+BBBFFFFFFFFFFFIIBBIFIBBBFFFFFBFFIIIIBFFIIBFBFFFFFFIIIIFFF7BB<<BBFB<BBBBFFB7<00B<BBFF00<BBFFFF''77B
+@HISEQ:105:C2UE1ACXX:3:1101:6941:23338_GTT 1:N:0:CAGATC
+AGAAAGGTTTTAAGTTGGTTGGGAATATAGGGGTTTTTTAGAGTTTTTATTAGGAGTTATAGTGTGTTGAATTTGGTTTTGGGTGTTGATTATAGGTT
++
+BBBFFFFFFFFFFBFFBBFFBBBFFFFIIBFFFIFFIIIIBFFFFFIIIIIIB<FFFFFFFBFBFFFF<BBFFF<<BBBB700<BBB0BBFFFF00<B
+@HISEQ:105:C2UE1ACXX:3:1101:10069:23622_TTT 1:N:0:CAGATC
+TTTTGTTTTAGGGTTTTATTTTTGTGTTTTATTTTTATTTTCGTATTATTAGTTTTTTTTATACGTTATTTGTAGAAGGTTAGTTTTTTTAATTTAGG
++
+BBBFBFFFFFBBBFFFIFIIIIIBFBFFIIBFIIIIIIIIIIBFFFIIIIIFFIIIIIIFFFFF<BFFFFF<BF<<B77BBB7BBFFFFFFFFFFF00
+@HISEQ:105:C2UE1ACXX:3:1101:14079:24078_AGT 1:N:0:CAGATC
+TTTAAAGTTTTTAGTTTTGAGTGGAATTTTAAGAATATTAGTGCGTTTTAAGCTTAGGTAGTTTTGGTAGTTTGAAAGTAATAGGGTGTATTTTGTAA
++
+BBBFFFFFFFFFFBFFFI<BBFFFFFFIIIIIBFFFIIIIBFFFFFFIIIIBFFIIBBFFFFFFI<7BFBFFF<BBF<BBFFF<07BBBBBFFF<BFF
+@HISEQ:105:C2UE1ACXX:3:1101:12064:24631_TTT 1:N:0:CCGATC
+TTATAGTGTATTTATATATATGAAATGAATTAATGAATTTTAAAAAAAAAGAAAGTAAGTTGTTTTTAGGATTGATATTTAGAGTTAATTTTTTGAGT
++
+BBBFFBFFFFFFFIIIIIIIIBFIFF0BFIIIIIBFFIIIIIIIIIIIIIBFIIFFFIBFFBFFFFFFB<BFF7BBFFFFF<B<BBBFFFFFFF'70<
+@HISEQ:105:C2UE1ACXX:3:1101:11630:24964_GAT 1:N:0:TAGATC
+TTAGTTTTTTTAGTGTTTTTTATTTATTTCGTTTTATTATTGGAGTTTGTTAAGAAAATTAGGGTTTGATTTGGATGTTAAGGATTGGTTTTTTTTTT
++
+BBBBFFFFFFFFBFFFFIIIIIIIIIIIIIBFFFIFIIIIIB7BFFFFBFFIIBFIIIIIFB<BFBFBFFFF<7BB<BBFF<7<BB77BBFFFFFFFF
+@HISEQ:105:C2UE1ACXX:3:1101:12594:24878_AAT 1:N:0:CCGATC
+TTTAATAGGATATGATATTATTTAATTTATAGATTATGGAAATTTTTTATATTTAATGAAGAAAGTTGGAATGTTTTGGGAGGTGTTTAGAATAAATA
++
+BBBFFFFB0BFFF<FFIIIFIIFIIIFIFIF<BFFFF<<BFFFIIIIIIIIIIIIIIBFFBFFIBFB7'<FF<<BBF0''77<BFB<BB0<BBBBBFF
+@HISEQ:105:C2UE1ACXX:3:1101:4483:25030_ATT 1:N:0:CAGATC
+AGGATGGTGTTTTTATTTTTAGATTTATATTATTTTGTTATATTTGTATTTGAGTAAGTTTATGGGTTTTTTAAAGAGGTAGGAGGAAGTTTTTTGTT
++
+B<BFFFFFFFFFFIIIIIIIIBBFIIFIIIIFIIII<BFFIFFIIBFIIII<BBFFIFIFIIIB7<FFFIIIFBF<<77BB00<70<B7BBBFFF0BB
+@HISEQ:105:C2UE1ACXX:3:1101:12198:25235_TTA 1:N:0:CAGATC
+ATATATGTAGTTTGTATTATTTTTGTTATAGTATATAAAGGTTAAAGAGTAGTTGTTTTAATTTTAGAGGTGGAGATTGGGTTGTATAGTTTTGGTTT
++
+BBBFFFBFFFFFFBFFIIFIIIII0BF<FF0BFFFIFFIBBFBFII<FBFBBFF7FFFIIIIIIII<F7<B'<BB7<B''0<<0<BBB77<BB'0<<B
+@HISEQ:105:C2UE1ACXX:3:1101:20477:25084_TGT 1:N:0:CATATC
+AGAGTTTATTGAGAAGTAAAGTATTAATTTTATGGGAGAAATGGGATAGAGGTAGTAGAAGTTGTTATGGAATGGGATTAATTAGGAAGTTAATTAAG
++
+BBBFFFFFFF0BBFFBFFIIFFIIIIIIIIIFIBB7FFFFIIFFBFFIFIBFFFFIIFIIFFFBFFFF77BFF<<B<FFFFFFF70<B7BBFFFFFF7
+@HISEQ:105:C2UE1ACXX:3:1101:5725:25359_TAG 1:N:0:CAGATC
+GAGAAATAAGATAATAAAGTAATAGTTGTGATTAGGAGGTTTTTTATAAGTTGATGGTTTATGTTAAGTAAGTTTATTAAGAAGTATAGTATTATATA
++
+BBBFFFFFFBFFFIIIIIIIIIIIBIFBFFFFIIBBFFFFFFFIIIIIIFFFBFFBBFFFIIBFFIIBFIIFIFIFFFFF<BF<BBFF7<<FFFFFFF
+@HISEQ:105:C2UE1ACXX:3:1101:5502:25591_AGA 1:N:0:CAGATC
+ATATGATTTTATTTTTAGGGATAATATTTTTTAAGTGAATTTTGATTTTTTGGTTAGTTATTTTGATGATGTGTAGAGGGTGTATAGTTTTTGGATAT
++
+BBBFBFFFFFFFFIIIIBBBFFFIIIIIIIIIIIBFFFIIIIIBFFIIIII<7BFFBFFFIIII<FF<FFBFFFFFB<<<BFBBBF<<BFFF00<BFB
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/out_paired.log	Wed Jan 10 19:09:42 2018 -0500
@@ -0,0 +1,37 @@
+# output generated by extract --bc-pattern=NNNXXX --stdin=input_read1.gz --read2-in=input_read2.gz --stdout out1.gz --read2-out=out2.gz --log=/tmp/tmpAvWtMd/files/000/dataset_20.dat
+# job started at Wed Jan  3 17:06:39 2018 on tzk-ThinkPad-T450s -- 9eecaab6-9562-4b29-8a2e-ae6c1034a14c
+# pid: 22903, system: Linux 4.10.0-42-generic #46~16.04.1-Ubuntu SMP Mon Dec 4 15:57:59 UTC 2017 x86_64
+# blacklist                               : None
+# compresslevel                           : 6
+# error_correct_cell                      : False
+# extract_method                          : string
+# filter_cell_barcode                     : None
+# filter_cell_barcodes                    : False
+# log2stderr                              : False
+# loglevel                                : 1
+# pattern                                 : NNNXXX
+# pattern2                                : None
+# prime3                                  : None
+# quality_encoding                        : None
+# quality_filter_mask                     : None
+# quality_filter_threshold                : None
+# random_seed                             : None
+# read2_in                                : input_read2.gz
+# read2_out                               : out2.gz
+# read2_stdout                            : False
+# reads_subset                            : None
+# reconcile                               : False
+# retain_umi                              : None
+# short_help                              : None
+# stderr                                  : <_io.TextIOWrapper name='<stderr>' mode='w' encoding='UTF-8'>
+# stdin                                   : <_io.TextIOWrapper name='input_read1.gz' encoding='ascii'>
+# stdlog                                  : <_io.TextIOWrapper name='/tmp/tmpAvWtMd/files/000/dataset_20.dat' mode='a' encoding='UTF-8'>
+# stdout                                  : <_io.TextIOWrapper name='out1.gz' encoding='ascii'>
+# timeit_file                             : None
+# timeit_header                           : None
+# timeit_name                             : all
+# whitelist                               : None
+2018-01-03 17:06:39,365 INFO Starting barcode extraction
+2018-01-03 17:06:39,377 INFO Input Reads: 100
+2018-01-03 17:06:39,377 INFO Reads output: 100
+# job finished in 0 seconds at Wed Jan  3 17:06:39 2018 --  0.60  0.05  0.00  0.00 -- 9eecaab6-9562-4b29-8a2e-ae6c1034a14c
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/out_single.log	Wed Jan 10 19:09:42 2018 -0500
@@ -0,0 +1,38 @@
+# output generated by extract --bc-pattern=XXXNNN --stdin=/tmp/tmpAvWtMd/files/000/dataset_13.dat --stdout /tmp/tmpAvWtMd/files/000/dataset_14.dat --3prime --quality-filter-threshold 10 --quality-encoding phred33 --log=/tmp/tmpAvWtMd/files/000/dataset_15.dat
+# job started at Wed Jan  3 17:06:20 2018 on tzk-ThinkPad-T450s -- 8897b3b4-c6e2-4ee3-b6c0-71044a63b7e1
+# pid: 22794, system: Linux 4.10.0-42-generic #46~16.04.1-Ubuntu SMP Mon Dec 4 15:57:59 UTC 2017 x86_64
+# blacklist                               : None
+# compresslevel                           : 6
+# error_correct_cell                      : False
+# extract_method                          : string
+# filter_cell_barcode                     : None
+# filter_cell_barcodes                    : False
+# log2stderr                              : False
+# loglevel                                : 1
+# pattern                                 : XXXNNN
+# pattern2                                : None
+# prime3                                  : True
+# quality_encoding                        : phred33
+# quality_filter_mask                     : None
+# quality_filter_threshold                : 10
+# random_seed                             : None
+# read2_in                                : None
+# read2_out                               : False
+# read2_stdout                            : False
+# reads_subset                            : None
+# reconcile                               : False
+# retain_umi                              : None
+# short_help                              : None
+# stderr                                  : <_io.TextIOWrapper name='<stderr>' mode='w' encoding='UTF-8'>
+# stdin                                   : <_io.TextIOWrapper name='/tmp/tmpAvWtMd/files/000/dataset_13.dat' mode='r' encoding='UTF-8'>
+# stdlog                                  : <_io.TextIOWrapper name='/tmp/tmpAvWtMd/files/000/dataset_15.dat' mode='a' encoding='UTF-8'>
+# stdout                                  : <_io.TextIOWrapper name='/tmp/tmpAvWtMd/files/000/dataset_14.dat' mode='w' encoding='UTF-8'>
+# timeit_file                             : None
+# timeit_header                           : None
+# timeit_name                             : all
+# whitelist                               : None
+2018-01-03 17:06:20,360 INFO Starting barcode extraction
+2018-01-03 17:06:20,362 INFO Input Reads: 100
+2018-01-03 17:06:20,362 INFO Reads output: 72
+2018-01-03 17:06:20,362 INFO filtered: umi quality: 28
+# job finished in 0 seconds at Wed Jan  3 17:06:20 2018 --  0.58  0.06  0.00  0.00 -- 8897b3b4-c6e2-4ee3-b6c0-71044a63b7e1
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/t_R1.fastq	Wed Jan 10 19:09:42 2018 -0500
@@ -0,0 +1,400 @@
+@HISEQ:105:C2UE1ACXX:3:1101:11160:2245 1:N:0:CAGATC
+AAAAGTAGTTAATATATTAGATTTGTTTGATAGTGGTAGTATATATTTTTTATTTTAGTATTTAGGAGGTAGAGGTAGATGAATTTTTGAGTTTAAAGTTA
++
+BBBFFFFBFFFFFIIIIIIBIIIIBIFFBFIIBBFFFFFFFFIIIIIIIIIIIIIIIBFIIIIIB7BBBFBBFFFF77<F7BFFFFFF7B7BBFFFF7<BF
+@HISEQ:105:C2UE1ACXX:3:1101:19338:2197 1:N:0:CAGATC
+TTTTTTTTTAGAGGGATTAGTTTTTTTTATTGAGGTTTTTGAAAGTTGTTGTATGTTAATTGTTTTTAGAATGTTGGGTATAAGTAGGATTTAGGTCTATT
++
+BBBFFFFFFB0<BF7BBBF7BFFIIIII7BF'0<0BBFFF'<BB'<B7<B07<B7<BFBBF0<BBBBB0<<B0BB<<000<BF00<<'0<BBB0'00BBF#
+@HISEQ:105:C2UE1ACXX:3:1101:19467:2281 1:N:0:CATATC
+TTGGTTAGGGTGAGATGTATAGTTTGGATTTTAGTGATTTTTGTAAAGGGGGAAAAGAATGGAGTTTTGGGTGTAGTGAGAGGTTATAGGAGTAGGGATTT
++
+<B<<FFFBBFFFFFFFFFIFIBFFI<<BFFFIIBBBFFIIIIBFFIIBBF7BBFFFBFFF77BBFBFF777BBBBBB<<<B<7<7BBF77<7<700007BB
+@HISEQ:105:C2UE1ACXX:3:1101:7009:2740 1:N:0:CAGATC
+AAGTTTTGTTTTTTATTTGGAGGTTATGGAATGTTAAGTAAGGTTTTTTTGGGTTTTGTTATTTATTTGATAATTGTGATTGTAATGTTAATAAGGGATAG
++
+BBBFFFFFFFFFFIIIIIB<FFFFFFIBBFIIFFFIIFIIIFFIFFIIII<0<BBFFBFFFFFFFFFF<BBFFFF<BBBBF7BFFF<BBFFFFF<00<BB0
+@HISEQ:105:C2UE1ACXX:3:1101:13708:2613 1:N:0:CAGATC
+ATATAATAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAAAGATAATTAATTTTTAAAATTTTTTTTTTTTTAATAAAA
++
+BBBFFFFFBFFFFIIIIIIIIIIIFFFFFB7B007BB0''''''0077BFF7'077BF0''0''''''00''00''''00'<BBBFBBFFFFF########
+@HISEQ:105:C2UE1ACXX:3:1101:19067:2707 1:N:0:CAGATC
+GTTTTTTTATTTGATATTTTAAAGGTTTTTTTTTTTTTTTTAGAAAATTTTTTTTAGTAAGATAGATTTTAAAGGGTTTGTTTTTTTTTTTTTTTTTTTTT
++
+BBBFFFFFFFFFBBFFFFIIIFIFFIFFIIIIIIIFFFFFB7'0<B0<BBFF'7<0'0<<''0<'0'0<BBB<<B'7'0'0'0<BFFFFFFFFFFFFFFFF
+@HISEQ:105:C2UE1ACXX:3:1101:4999:3182 1:N:0:CAGATC
+GTTTTATGAGGATTTTAGGGGAGTGATTGTTTAAAGTTTATAAGATTTATGATTTATATATAGTTAGAATAGTATGTGTTAAATAAATATAAAGGGAGAGG
++
+<BBFFFFBFFFFFFIIFBBBFFFIIFFIBFFIFIIBFFIIIIIFFIIIIIBFFIIIIIIIIIBFFIBFIII<FFFBFBFFFFFFFFFFFFFFF<07<7<<7
+@HISEQ:105:C2UE1ACXX:3:1101:16790:3145 1:N:0:CAGATC
+TATAGAGGTATTTTGTTATTTTGTTTTAGTTATTGCGGGTTAGAGTAGATGGTTATTTTTAGTAGAGTATTGTTTGTTGTTTTTTATATGTGGTATAGAGG
++
+BBBF<BBFFFFFFI<FFFIFIIBBFFIIBFFFFI<BBBFFFF<FFFF7FF70<BFFIFIIF7BFBB<FFBF'BBF'<<0<BFFBBBBBF'<70<<<B####
+@HISEQ:105:C2UE1ACXX:3:1101:18065:3106 1:N:0:CAGATC
+AAGTATTTGTTATATATATTTTAAAGTTTTTTTTTTTTTTAGGAATTTTTTTTTATAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTTT
++
+BBBFFFFFBFFFFIIIIFIIIIIIIFIFIIIIIIIIIIFF''00<<BB'<<'7''000<BFFF77<77BFF77BFBB07<7<BFFFFFFFF##########
+@HISEQ:105:C2UE1ACXX:3:1101:2300:3263 1:N:0:TAGATC
+TTATTTTTAATAAAATTTTTATTATTTAATTTATTAGTTAATATTTAGGAGTTTTATGTTGTGGTAAAATTTTGTTAGAGAGATAGAGAAAGTATTTAGTT
++
+BBBFFFFFFFFBFFIIIIIIBIBBFFI0<FFFBFF70B00<'0<FFFBBBBFF''0B0BB000'7BBFFFFII'<BF0<'<''07'70BBF'7BBBFB7<B
+@HISEQ:105:C2UE1ACXX:3:1101:5605:3427 1:N:0:CAGATC
+AGATGAGAGGTATAGGATGTGGGGAGTTTTAGTAAGATTTATAGATAAGAAGTGGTTCGGTTATAGGATTTGTTTTAGATTTTTAGATTTTTTTGTGTAGA
++
+BBBFBFFFFFFFFFFBFFFFIIIIIIIBFFFBFFIBFFIIIIIFFIIIFFIBF7BFBFBBFFIFF<<FFFFBBFFBF0<BFFFFF7<BFFFFFF0B7BF0<
+@HISEQ:105:C2UE1ACXX:3:1101:8129:3589 1:N:0:CAGATC
+TTTTAGTTTTTAGTTAGGATTATACGTTTATTGTGATAAAAGAGTTTTTTGATTTATCGGGTTATGTTAGGGTTTATTGATATTAGGGAATTTGAAGAGAT
++
+BBBFFBFFFFFFBFFFBBFIIFIIFBFFFFII<BBFFFIIIBFFIFIIIIBBFFIIII<7<FBFF<FBF<7<BBFFFF7<BFFFF700<BBFF0<B'<07B
+@HISEQ:105:C2UE1ACXX:3:1101:14304:3866 1:N:0:CAGATC
+GTGTTTATATAGGGGATTTTTGAGTTTGATAGGTTGTTTTTGTAGAGGGTAGAATTTTGTGGAAATGTTGGTATTGGTTAAGGGGTTTTAGTGAAGAATAA
++
+<BBFFFFFFFFFFFFFFFFII<BBFFFBFFIFBFFBFFFII7FFFFFFFIF<BFIIII<F<BBFIFBFB7<BFFFBBBBBF777<B7BBF7B<BB7BBFFF
+@HISEQ:105:C2UE1ACXX:3:1101:12720:4398 1:N:0:CAGATC
+GTATGTGTGTGTGTGTGTGTATTTAATTGAAGTTGGGTTTGGTGATATATATGTTTAATTTTAGTATTTTAGTGGTAGAGGTAGGTTAATTTTTGTTGAGT
++
+<BBFBFFFFFFFFIFIFIFFFFFIIFII0BFBFFB7BFFF<BFFFFIIIIIIBFIIIIFIIII<FIFIIIFBFBBFB7B<<BB7<B<BFFFFFF0<B0<7B
+@HISEQ:105:C2UE1ACXX:3:1101:14945:4439 1:N:0:CAGATC
+AGTGTTGAGTGGAGTATTAGAGAAGAGAAATAAGATAATAAAGTAATAGTTGTGATTAGGAGGTTTTTATAAGTTGATGGTTTATGTTAAGTAAGTTTATT
++
+BBBBFFBFFFFFFFFFFIIBIFFIFFBFFIIIIBFFIIIIIIBFFIIIBFFBFFFIIIFBFFBFBFFIIIIIFFFBBF77BBFFF7BBFF<BBF<<BFFFB
+@HISEQ:105:C2UE1ACXX:3:1101:8616:4508 1:N:0:CAGATC
+AGAAATTTTGGGGGTGTAGGAGTGGTAGGATAGGAGTGTTGTTTTGTAATAGTTTTTTTTGAGGTTTAATAGGTAGGGTAGTTATTTTTAGTATTGTAAGG
++
+BBBFFFFFFBBBFFFIIFFFIFFFIIFFIIFIFBFFFIIF<FBFI<FFIIIBFFFFFFFF077<B<BBBFF<<BBBBBB<7B<BFFFFFF0BBFF0<BF00
+@HISEQ:105:C2UE1ACXX:3:1101:18975:4834 1:N:0:CAGATC
+TATAAATGGTAATTTTGTAATTTAAAGATTTAAAAGTAATTATTGGTAATAGTTATTTGTGGGAGGTTGAGGTAGGGGGATTTTTGTAGAGATCGGAAGAG
++
+BBBFFFFBBFFFFIIIBFIIIIIFIIFFFFIFIIIBFIIIIIIIB<FFIFIBFFFIIIBFFBFFFII<<BFBFFFFIFF<BBBFF0BB7<<<BF707B0B0
+@HISEQ:105:C2UE1ACXX:3:1101:4984:5374 1:N:0:CAGATC
+TGTATCGAGGTTTGAATGAGAGTGGTATTTTTGTTATTTGTTAGTTAATGGTTTTGAGTATTAGTTTGGAAAATGATAATAAGTATTAGTTGAGGTGTTTG
++
+B<BFFFBFFFFBF<FFFBFFFFFFFIFFFIII<BFFIIIBFFFFFFIIIBBFFFIBBBFFFIIBFFF<<BFFFFBBFFFFFF<BBFFF<BB7<7<BBBBF0
+@HISEQ:105:C2UE1ACXX:3:1101:14432:5660 1:N:0:CAGATC
+TAAAATAAAAGATAGAGATAGAATAAAAGATAGAGATTTTAATTTTTCGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATAAA
++
+BBBFFFFFFFBFFIBFFFIIBIIIIIIIBFFIBFBFFFFIFFIIIIIFBFFIIIIIIFFFFFFFFFFFFFFFFFFFFF<BFBBBBBFFFB###########
+@HISEQ:105:C2UE1ACXX:3:1101:17410:5545 1:N:0:CAGATC
+ATTATTTTTTTGAAGTTGTTAGGGTTATTTAGTGAAATTTGAGTATATTTAATTATTTTAGATGTTAGTTTTATTTAGGTTTTATTTTATTAGAGGTTTGG
++
+BBBFFFFFFFB<FFFIFBFFFBBBFFFFIII<FBFIIIII7FBFFFIIIIIIIIIIIIIF<FF<FFFBFFIFFFFFF77BBFFFFFFFFFFF'<70<BB##
+@HISEQ:105:C2UE1ACXX:3:1101:12336:6058 1:N:0:CAGATC
+ATTTAGATGATGGTTTTTTTATTTGATATTTTAAAGGTTTTTTTTTTTTTTTTTTAAAAAATTTTTTTTATAAAAATAAATTTTAAAGGGTTTTTTTTTTT
++
+BBBFFBFFBFFBBFFFIIIIIIIIBFFIIIIIIIIBFFFFIIIIIFFFFFFFFF700<BF0BBFFF0<70''00'0'000'<<'<B<00'0'00007<BFF
+@HISEQ:105:C2UE1ACXX:3:1101:5999:6265 1:N:0:CAGATC
+TATATTGTTATATATTTGTGTTTTTTTTGAATTTATTTTTTGTTTATTAGTTTTTGTGGTTGATAGGGTTTTTGGATATTGGGTTGTTTTTATATATAATG
++
+BBBFFFBFFFFFFIIIIBIFIFIIIIII<BFFIIFIIIIII7BFFFIIFBFFIII<B<BFB<BBF<BBBBBFF00<BFFF700B<7<BFFFFFFFFFFFF0
+@HISEQ:105:C2UE1ACXX:3:1101:5836:6568 1:N:0:CAGATC
+ATTTGGATGTTAAGGATTGGTTTTTTTTTTGATTTAGGGTATTGTTAGATAGTATAGATTTTACGGAGTTTTGTATTTGAAAGGTTGTTGGTATGTTTTTT
++
+BBBFBBFFFFFFFFFFFFBFIFIIIIIIII'0<FFB<BBFFBF7BFF7BFF<BBFF<BFFFFFF<<BBFBBF7BBBFF'<BB0'<B0<B0'7<B<BFFFFF
+@HISEQ:105:C2UE1ACXX:3:1101:15437:6678 1:N:0:CAGATC
+AAAATAAGAAATTAGTTAAGAGGAGGAGGTTGTAGTGGGGGTAAGGAAGGAGAGTTTTTGCAAGTTTGGAAATTTGAAAATTATTGTTTTTTATGTTTAGA
++
+BBBFFFFBFFFFFIFFFIIBFFFFFIIIIIB<FFFFFIIIIFBFBBFFFFFFFFFBFFF<BFFBFBF<7BBFFFF<BBFFFFFFF7BBFFFFFF0BBFF##
+@HISEQ:105:C2UE1ACXX:3:1101:12432:6996 1:N:0:CAGATC
+AAGTTATTTATGAGATTAGTTTATAGTAAATTAAAAAGGGTCTTAGTATTTTGATTATGAAATTAATTATTTTAGTAATATTTGAAGTTGTAATTTTAGAT
++
+BBBFFFFFFFFBFBFFIIBIIIIIIBFIIIIIIIIIIFBFFFFFIFFIIIIIBFFIIIBFIIIIIIIIIIIIIIBFFIIIIII7BF<BF<BFFFFFFF0BB
+@HISEQ:105:C2UE1ACXX:3:1101:19878:7022 1:N:0:CATATC
+GTTGTATTCGCGTTGTTTTTGTAGGGTTTATGTATTTTTTTTTTTGGTGGAGAGTTTCGTTGAGTGATTTTTTAGTTCGTTTTTCGTTTATTATTTTTCTT
++
+<BBBFFFFFBFBFFBFFIII<BFBFFFFFFF'BBFIIIIIIIIFF''00'7'<7<<B<'7B'0'<0<<BFFFF7'<B<'7'7BB7'0BB0BB<'0BB####
+@HISEQ:105:C2UE1ACXX:3:1101:6837:7574 1:N:0:CAGATC
+TTTGAAGATTTTATCGTTAGTTTTTTAGTTTTTATTTGGAGTTGATTTTGAGGTGTTAGTTGTTGTTGTTTTTGTTAGAGGTTAGGTTGTTTTTTATTTGG
++
+BBBBFFFFFFFFFIIBFFIBFFIIIIF<FFIIIFFII<0BBFBBFFFIIBBBBFFFFFBFF<FF<FF<BFFFF7BBB7<77B<B<7<BBBFFFFFFFFF##
+@HISEQ:105:C2UE1ACXX:3:1101:15853:7717 1:N:0:CAGATC
+GTTTTTTTTTTATATTGTGGTGATAAGTGTTTAAGGTGGGTGAAGGTAGAATTAATCGTAGTTGTGGCTGTTAGTGTTTAGTATATTGTTTTTTGTTAAGG
++
+BBBFFFFFFFFFFFFI<BBFFFBBFFBB7BBFFFB<BFFIIFBFBBBF<BFFFFFFF7<B<BB7<BB'7<BBB<BB<BBB0<<BFFF'<BBFFF'<BBF##
+@HISEQ:105:C2UE1ACXX:3:1101:2440:7931 1:N:0:CAGATC
+ATTTGAAAAGTGTTATAGATATTAGAAAGGTATGGAGATGAGAGGTATAGGATGTGGGGAGTTTTAGTAAGATTTATAAGATCGGAAGAGCACACGTCTGA
++
+BBBFBFFFFFFFFFIIIBFFIIIIBFFIBBFFIBBFFIFFFFFFFIFFIBBFFFFFFFIFIIBFFIBFFFBFFFFFFFF7BBF70<B0<7<BBBF0<BF7<
+@HISEQ:105:C2UE1ACXX:3:1101:6407:7896 1:N:0:CAGATC
+TTTATAGGTTTGGGTTGAGGATATGTATTGGGAGTTATTAGAGGGTTATAGTTAGGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATC
++
+BBBFFFBBFFF<BBFFBFFFFFIIBFFIIBBBFFFFFIFFBFFFFIBFFIBFFIBBFBFFFFI<BBF<F<BFFFFBFBF<BBFFFFB<BBFFFB0<BFFBB
+@HISEQ:105:C2UE1ACXX:3:1101:19497:7952 1:N:0:CAGATC
+ATACGTAGATGTTTAAAAGTTTAAATAAATGTATTTAGAGGTTTATGAGGTTTATGGTTAAGTGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC
++
+BBBFBFFBFFBFFFIIIIBIIIIIIIIIII<BFFIIFBFBFFFFFIBFBBFFFIIB7FFFIBFBBBFFFFFBBBFB<0<BFFF7<BB7BBBFFFF<BBFFB
+@HISEQ:105:C2UE1ACXX:3:1101:3853:8232 1:N:0:CAGATC
+GGTTAGGTTGTTGTTGTATTTAGAGGTTTTTAGGAGTTGTTTTTGAGTTGTTGTTTATTTAGTTTGTCGGTAGAGATCGGAAGAGCACACGTCTGAACTCC
++
+<<BBFBFFFBFFBFFBFFFIIF<FBFIFFIIFBBFFIFBFFFIIBFBFFBFFFFFIFIIII<FFIBFI<<BF<BB<BF70<B0<7BBFFF0BBB7BBFFFF
+@HISEQ:105:C2UE1ACXX:3:1101:7442:8079 1:N:0:CAGATC
+GGTGTTTGATTTTTTTGAAATTATGTCGGGGTATATTGAGGGTTAGGTTTTATGGGTAGTATTTTATTATTTTTTTGTTGTTTGGAGTTTCGGTTTTGGAT
++
+BBBFFFFBFFFFFFIIBFFIIIII<FFBBFFFFFIFF7<<BFF<BBBFFFIFF7<BFFBFFFFFFFFFFFFFFFFF0<B0BBB'000<7BF00<<<B''0B
+@HISEQ:105:C2UE1ACXX:3:1101:14324:8171 1:N:0:CAGATC
+TGGTTGTAGTTTTTTATTGGTGTGTGTGTGTTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGAGTGATAGTTTGTTGGGGATTGTTTTTTGTAGGACG
++
+B<<FFBFFFFFFFIIFII<<BFFFIFFFFFFF<FBFFFFIFIIFFIFIIIFIIIFIFFFFFFFFFBBB<BBB7BBB0<B70''7<<'<BBFF#########
+@HISEQ:105:C2UE1ACXX:3:1101:3147:8365 1:N:0:CAGATC
+AGTTATAATTGTTTTAAGAGTTATCGTGTGGGTATTGGGAGATTGGATGAATGAAAAGTGTATTTAATTGGTGAGTTATATTTTTTGTTCTTTGGTTTTCG
++
+B<BFFFFFFFBFFFIIIBFFFIFIIBFBFFFFIFFIB<BFFFFFB7BFBFFIBFFII<FBFFFIIIIII<7BBFFBBBFFFFFFFF0BBFFFF0'7BBFF#
+@HISEQ:105:C2UE1ACXX:3:1101:7150:8384 1:N:0:CAGATC
+GAGTAGTAGTTTTTGTGTAGGTTTTGGTTTGTGTGGTATTAGTAGGTAGTAGTGTGTTAGTTGGAGCGATAGTAGTAAGTTATGGTTATTGTTTATTATTA
++
+BBBFFFFFFFFFFIBFBFFFFFFFFB<FFF<BBFFFFFFIFBFIFFFIIIIFFFIFIFF<FF7<BFFFFFFBBFBBBF<BBFF77<BBFF7BBFFFFFFFB
+@HISEQ:105:C2UE1ACXX:3:1101:11512:8610 1:N:0:CAGATC
+TGTGTTTTGAAGTTGGCGGAGTTCGCGTTTTTAGGAATTTTGTTTTGGAACGGTTTGGTCGGGGTTTTCGTTTTTTTTTGTAGAAGTGTAGGAGATGTTGT
++
+B<BFFFFF<FFBFFBBFFFFBFFFBFFFFFIII<7BFFIII7BFFI00<BFBBBBB77<B0<<<B7BBB7BFFFFFFFF'7<000'<'0B'0<<BB0<B<B
+@HISEQ:105:C2UE1ACXX:3:1101:18380:8734 1:N:0:CAGATC
+GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTTTGTTTAAAATTTTTTTTTTTTTTTTTTTTCATTTTTTTTTTTAAAAAATTTTTTTTTTTAAATTT
++
+<BBFFFFFFFFFFIIIFFFFFFFFFFFF0''0''''''00'07B<'0<BFFBB77BFB###########################################
+@HISEQ:105:C2UE1ACXX:3:1101:18629:8512 1:N:0:CAGATC
+TGAATTAATGAATTTTAAAAAAAAAGAAAGTAAGTTGTTTTTAGGATTGATATTTAGAGTTAATTTTTTGAGTTTTTTTGTTTGTTTAGGTTTTGGTTTAT
++
+B<BFFFFFFBFFFIIIIIIIIIIII<FFIBFFFBFF7FFFIIF<<BBF7BFFIIIF7B<BBFFFFFFFF0<7BBBFFFF'0BB'0<B<'0<<B'''7BBBB
+@HISEQ:105:C2UE1ACXX:3:1101:5146:8981 1:N:0:CAGATC
+TTTAGGTTTTAGTTGTTGTTGTTTGTTTTGTATTAGTGTAGTTATAATTTTTTTGTTGATTTTGTTGTTTTTAGAAGGTTTGGGGTGAGTTTAGTATGAGT
++
+BBBFBBFFFFFBFF<FF<BFBFFI0BFFI0BFFFF<BBFFFFFFIIIIIIIIII7BF7BFIII<BF7BFFFFF0<<7'<<B00'0<<77BBBF'<BB0<0B
+@HISEQ:105:C2UE1ACXX:3:1101:3344:9104 1:N:0:CAGATC
+TAGATGTTGAGTGTTTGTTTTGTGTTAGGTATTAGGTGGGATTAGGTGGTTATTATCGAGTTTTTAGGAGAGAGTATAGTTGTAAGCGGACGATAATAATT
++
+BB<FFFFFBFBFFFFFBFFIIBFBFFFBBFFFFFBBFFFIIFFFFBFFFIFFIIIII<FBFBFIIF<<BBFFFFFBBF<BB7<BF0<007B7<BFFFFFFF
+@HISEQ:105:C2UE1ACXX:3:1101:8326:9445 1:N:0:CAGATC
+TTTAGGAATTATATATTTTTTATATATTATTAAAGTGATTGGTTAGGAAATTAAAGTTTATTTGGAAGGTATTGTTTTTGAAAATGAAGTTATGTTTTTGG
++
+BBBFB<FFFFFFFIIIIIIIIIIIIIIIIIIIIIBFFFFI<BFFFBBFFIFIIIIFIFIFIII<7BFBBFFFFBFFFIF7BFFFF7BB<BBFF<BBFFF##
+@HISEQ:105:C2UE1ACXX:3:1101:2097:9536 1:N:0:CAGATC
+TTGGATTTTTTGGTTTGATAAGGAGTTTTGTAGGTTAAAGAATGTTTTTTAAGGGATTTTTGTTATGTGATTAGTTAATGGGATTTTATTTAAGTTTGGTT
++
+BB<BFFFFFFFBBFFFBFFIIFBFFIFFIBFIFFFFFIIBFFIFFFIIIIII<<BFFFIFF<FFFFBFBFFFFBFBFFF700<BFFFFFFFFF<BBF<0BB
+@HISEQ:105:C2UE1ACXX:3:1101:16351:9944 1:N:0:CAGATC
+TTGGACGGGATGGTTGTGGTAAAGTTTTAATAGTTTTATTGGGGGATGGTATAGAAGTAGTTTAGGTTGTATTGTGGGATTTATTTTTATTGTTTGTTTTG
++
+<B<<FFFFFFFFFIFBFFFFFIIBFFIIIIIIBFFFIFFI70BFFFFFFFFFIBFFBFFBFFFFBBBB<BFFF<B<BBBBBBFFFFFFFFF0<BF0BBFF0
+@HISEQ:105:C2UE1ACXX:3:1101:17628:10309 1:N:0:CAGATC
+TGTATGTTTAGATTTGTTTTGAATTTGCGTTTTTGGTTGTTGATTATTTTTTATTATTTTTTGTTTTTTCGTGTGTTCGGGAGATCGGAAGAGCACACGTC
++
+B<BFFBFFFFBFFIIBFFFI<BFFFI<F<FFFII<0BF<FF0BFFFIIIIIIIIIIIIIIIF7BFFFFFF7B7B<BBB7''707BF007B0<07<BBF0<B
+@HISEQ:105:C2UE1ACXX:3:1101:1440:10515 1:N:0:CAGATC
+AGAGTGTTTTTAGTGTGAGGAGTTGGATGTTTTAGATATTTATTTTTATATTGTTTATTTAGGTTATGATTTATGTTTTATTTTAGTTTAATTTTTTGTGT
++
+BBBFFFFFFFFFBFBFFFFIIIIFBBFFFFFFIF<BFFIIIFIIIIIFFFFIBFFIFIIIFBBFFFF<BFFIIIBFFFIIIIFFF7BFFFFFFFFFF7B<B
+@HISEQ:105:C2UE1ACXX:3:1101:11787:10735 1:N:0:CAGATC
+AGAATAGGGAAGTGTATAGAAGTGATATATAGTTTAGATTTGAGTTTTTATTTTGTTTTTTATATTTTTAGATATAAGTAGTTATTAGATTTTTTTGAATT
++
+B<BFFFBBBFFFFFIFFIBFIBFFIFIIIIIBFFIIBFFIIBFFFFFIIIIIIIBFFIIIIIIIIIIIII<FFIIIF<FF<BFFFFF0BFFFFFFF0<BFF
+@HISEQ:105:C2UE1ACXX:3:1101:10231:11124 1:N:0:CAGATC
+TTGTTTTAGTTATTTTTTGGTTTTTTAGATTTTATGTTTTTAGTTTTTATAATTTTTATATTGTATTTGTTTATTAAGTGTGAAATGTTTTTGTAGGATGT
++
+BB<FFFFFBFFFFIIIIIBBFFFFIIF<FFFIIFF<FFIIIFBFFFIIIIFFIIIIIIIIII<FFFFF<FFFFFFFF<B<B<BFFF<BBFFF0<B70<<7<
+@HISEQ:105:C2UE1ACXX:3:1101:12958:11220 1:N:0:CAGATC
+AATATAATTTAATAAGTAGTGTTTTTTTTTTTTTTTTTTTTTTAATAGTTTATTTTTTTTTAGGAAATTCAAAATTTAGTTTTTCTTTAATTTTATAATTA
++
+BBBFFFFFFFFFFIIFFFFIFIIFIIIIIIIIIIF'07<BFFB##########################################################
+@HISEQ:105:C2UE1ACXX:3:1101:9833:11396 1:N:0:CAGATC
+GTTTTTATTTTTGGGATTAAGGTAGTTGTTTTTTGATTTTTGTGTGTTATAGTAAGAATGATATAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
++
+<BBFFFFFFFFFBBBFFFIIFFFFFFIBFFIIII<BFFIII7FBFBFFIII<FFFBFIIBFFIIF<7BBBFF<7B<0<7<BFFF7BBF7BBFFFFF<BBBB
+@HISEQ:105:C2UE1ACXX:3:1101:13437:11566 1:N:0:CAGATC
+TTATTATTGTGGATTTTTTTAATAGTTTGAGAATGAGTTGATTTAGGGTTTTTATTTTATTTTTAAAATTAGGAGTATAATGGGTTTAAGATATAGTTTTT
++
+BBBFFFFFBFFFFFFIIIIIIIIIBFFF<BBFFF<FBFFBFFFIFB7BFBFFIIIIIIIIIIIIIFIFFFF<7BBBBBFFF700<<BFF<BBFFF<<BFFF
+@HISEQ:105:C2UE1ACXX:3:1101:12514:13081 1:N:0:CAGATC
+TTAAAGTTTTGATTTTAAATTTAAGTTAAGGTTTGGGGAGTTGATTTTTGTTTTGTGGGTTGTTTTTTTTGTAGGAGTTGGTTTTTAGAGGTTTTTAGGAA
++
+BBBFFBFFFF<FFFIIFIIIIIFIBFIIIBBFFFB<BFFFIBBFFIIII7FFII7F<<BFFBFFFFFFFF0<B77<0B700<<BFFB'<00<BBFFB####
+@HISEQ:105:C2UE1ACXX:3:1101:18902:13084 1:N:0:CAGATC
+AGGAGTCGGGTTTTGTGAAATATTATAGTAGAGATAGGTGTTGGTTTTGGAATGGAGATTTTTAGTTTTTTTTTATTTTGATAGTTTTGGTAGATCGGAAG
++
+BBBFFFFFFFFFFIBFBFIIIFFIIIIBFFBFFFFFFFIFFB7BFFFIB7<FB<BFFIFFFIIIBFFFFFFFFFBBFFF0<BF0<BFF0'<B0<BB#####
+@HISEQ:105:C2UE1ACXX:3:1101:3574:13312 1:N:0:GAGATC
+ATAAGTTTAGTAGTTAGGTAATCAGGTTTTTGTTTTATAATTTGAGTTGGTTAGAATGGAATGGGTATTGTAATGTTTGTTGGAATTTTTTGGTTTTATTT
++
+BBBFFFFFFFFF<FFFB<FIFIBFB<BBFFI<BFFIIIFIIFI<B<BF0<FFF7BBF0'BBF<77BFFF7BBFFBB<B0BB''7BBFFFFB0'7BBBBBB#
+@HISEQ:105:C2UE1ACXX:3:1101:5163:13594 1:N:0:CAGATC
+TTGTGGGTTAGAAATGGGTTATTTAGAGGAATATATAGTTAATATGGTAATGGTTATTTTTAGTTTATTAGTTTAAGTATTTATTTTTTTTTTATAGATCG
++
+BB<FFFFFFFBFFIIBBFFFFFIIIBFBBFFFIIIIIBFFFIIIIB7BFIIBBFFFIIIIII<FFIFIIIBFFIIIBFFFFFFFFFFFFFFFFFFF0<BB#
+@HISEQ:105:C2UE1ACXX:3:1101:10809:14594 1:N:0:CAGATC
+GTATTAGAGAAGAGAAATAAGATAATAAAGTAATAGTTGTGATTAGGAGGTTTTTTATAAGTTGATGGTTTATGTTAAGTAAGTTTATTAAGAAGTATAGT
++
+<BBFFFBFBFFFFFFFIIIIBFFIIIIIIBFFFIIBFF0BBFFFIBBFFFFFFFIIIIIIBFB7BFBBFFFII<BBFF<BBF<BBFFFFFF7<B<BBFF77
+@HISEQ:105:C2UE1ACXX:3:1101:7778:14963 1:N:0:CAGATC
+AGTGGATGATAATAATTTAGGTGATTTATAGTGAGATAAGAGGTATATGAATTTTAGTATTTATTTGTTTTTTTATTTTGGGTTATTTGAGGGTGTTTTAT
++
+BBBFBFFBFFFFFIIIIIIBBBFFFIFFIIBFBFBFFFIBFFFFFFIIBFIIIIIIBFIIIIIIIIBFIIIIIIFFFFF707<<BFFF7<''077<BBBFF
+@HISEQ:105:C2UE1ACXX:3:1101:2575:15554 1:N:0:CAGATC
+TTTGGGTTGGTTTTTTTTTTTTTTAAAAAGGTTTTAGGGTTTTGGTTTTTTTTATAATTTTATTAAAATATTAAATATTTATAAAATTTTTATTTTAAAAA
++
+BBBBBBFFBBFFFIIIIIIIIIIB7'0'00'7<'00<B0<<BB7'7BBFFFFB'0<'<BFB'<<BB'00'0<'0'00<'000'0B0<BFF<'<BF<BFB00
+@HISEQ:105:C2UE1ACXX:3:1101:20234:16099 1:N:0:CATATC
+TAAGAGGAAAGAATGGAGAAGAAGATTGGTTTTAGGTATATGATGGAATAGGGTAAAAATGATAGTTGAGGTTAATGGTTTAATTTTTGGGAATTATAGGT
++
+BBBBFFFFFFFFFIBBFFFFFFIBFFFB<BBFFFBBFFFFIBFFBBFFFFBBBFFFIIII<BFIBFF<BBFFFFFB77BBFFFFFFFF000<BBFFFF77<
+@HISEQ:105:C2UE1ACXX:3:1101:20842:16102 1:N:0:CAGATC
+TTGGGATTGTTTTTGTTTGAATTATTTTTTTGTATTATATTTTAATGTGTTGTTTATTTTTAGAATATGGAATTTGTAGGAAATTTTTAAAATTTATTTTA
++
+<B<BBFFFFFFFFIBFFIBFFIIIIIIIIII7BFFIIIIIIIIIIIFFFFFBFFIIIIIIII<BFFFF<7BBFFF<BB00<BBBFFFFFFFFFFFFFFFFF
+@HISEQ:105:C2UE1ACXX:3:1101:21194:16434 1:N:0:CAAATC
+TATAGGTTTTTATTTATGTAGTTTTTTGTATTTGTTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
++
+BBBF<<BBFFFFFIIFI<BFBFFFIII7BFFBF0BFFFI7BFFFIIFIFFFFFBFBBFFFBBBFFFBFFFFFFFFFFFFFFBFFB<<7BBFFFFFFFFBBB
+@HISEQ:105:C2UE1ACXX:3:1101:7164:16848 1:N:0:CAGATC
+TTTTTGAGTTTGATAGGTTGTTTTTGTAGAGGGTAGAATTTTGTGGAAACGTTGGTATTGGTTAAGGGGTTTTAGTGAAGAATAAATTTTTATGTTGTATA
++
+BBBFFBBBFFFBFFIBBFIBFFFII<BFBFFFIIFBFFFFIIBFB0BFFIBFF<<FFFI77BFFI<<<FF<BBF<B<<B7BBFFFFFFFFFFF'<B7BBFF
+@HISEQ:105:C2UE1ACXX:3:1101:2125:17112 1:N:0:CAGATC
+AAGAGGAGGAGGTTGTAGTGGGGTAAGGAAGGAGAGTTTTTGTAAGTTTGGAAATTTGAAAATTATTGTTTTTTATGTTTAGATTTTTTTATGACATTTGA
++
+BBBFFFFFFFFFFIBFFBFFIIFBBFF<FFF7B770BBFFI'7BF7BBF<'<F<FFF'<BFBFB<BF7BBFBBB<B'<BBB'7<BBBBBBBB'00<BBF##
+@HISEQ:105:C2UE1ACXX:3:1101:6615:17184 1:N:0:CAGATC
+GAGAGAGTTATATGTAATAGTTAGTTTTATTTAGAATATTAGAAGAATTATATGAGTAAGGTGTTTAATTATAAGGATAAGTTATAAGTTATAAAAATACG
++
+BBBFFFFFFFFFFBFFIIIFIIIBFFIIIIIII<FFIIIIIBFFFFIIIIIIIBFBFFIFFFFFFIIIIIIIIIBBBFFF<BFFIII<BFFFFFFFFFFF#
+@HISEQ:105:C2UE1ACXX:3:1101:12454:17532 1:N:0:CAGATC
+TTGGTGTAGAGGTTTGTTAAGTGTGGTATGGTTGAGAATATTCGTGTTTTATTGGTTAAATGTGATTTTTGTATTAAGTTGAGAGTTAGGGGTTTTGTTTT
++
+<B<<BFFFBFFFFFF<FFFFBFFIFFIFFBBFF<BBFFFFIIIFFFFFFIFIIB7FFFFII<FBFFIIII<FFFFFF<BB7B7B<B<B7''7<<BF<BBFF
+@HISEQ:105:C2UE1ACXX:3:1101:2584:17964 1:N:0:CAGATC
+TAGAGGGTTTTGTAGAGTATTTGAGTATTTGTTATTATTGTTGTATTTATAGTATTAATTTTTTTAGGAGTAAAGTAATTTTATTAAGTTTTAATAGATTT
++
+<B<FFFFFFFFBFFBFFFFFFIBFBFFFIIBFFFFIIIIBFFBFIIIIIIIBFIIIIIIIIIIIII<7BBFFFFFFBFFFFFFFFFF<BBFFFFFF7<BFF
+@HISEQ:105:C2UE1ACXX:3:1101:9624:17911 1:N:0:CAGATC
+ATTTTGATGTTATTATTTATTTATAAAGTAAGAATAAGGTTATTGGTTTTGTTGTTTTTTAGAGTTGTAAGGAGGATTTTATATAGTAAAAGATGTAGAAT
++
+BBBFFBFFBFFFFIIIIIIIIIIIIIIFFFFFFIIII<BFFFFIBBFFFIBFIBFFIIIIFBFBFB<FFIBBB<<BBFFFFFFFF7<BFFF<BB7BB7BBB
+@HISEQ:105:C2UE1ACXX:3:1101:19795:18124 1:N:0:CAGATC
+TTTATATTAATTTTTGGGTATTATTTTAGTGTGTTTAAGATGATATTTTAAGAGAAATTTGAATTGAAGAGATTAGTTTTTTATAAATGGCGTATGAAGGA
++
+BBBFFFFFFFFFFII<<<FFFFIIIIII<FBFFFFFIIBFFBBFIIIIIIIBFBFFIIIIBFFFI<BFBFBFFFIFFIIIFFFFFFFF7'70<BB7<B707
+@HISEQ:105:C2UE1ACXX:3:1101:2293:18893 1:N:0:CAGATC
+TGGATGCATGAAAAGTGTATTTAATTGGTGAGTTATATTTTTTGTCTTTTGGTTTTCGTTTTTAGATATTTATTTATTAGTTTTTATTGTTTTTAGTTTGA
++
+<<<BFBFFFBFFFIBFBFFFIIFIIIB<BBFBFFFFFIIIIIIBFFIIII70BFFII7BFIIII7BFFFFFFFFFFFFF7BBFFFFFF<BBFFFF7BBF0<
+@HISEQ:105:C2UE1ACXX:3:1101:12741:20220 1:N:0:CAGATC
+ATTTTTTTGGAAGAGAAGGAATTTATTTAGGGGAAATATATGTGTTTATATAGGGGATTTTTGAGTTTGATAGGTTGTTTTTGTAGAGGGTAGAATTTTGT
++
+BBBFFFFFB<FFBFFFFFFFFIIIIIIIIBBFFFFFIIIIIBFFFFIIIIII<77BBBFFFF7BBFBF<BBF<<B<<BBFFF7BF7B<'0<<0<BFFFF0<
+@HISEQ:105:C2UE1ACXX:3:1101:18080:20193 1:N:0:CAGATC
+TTATATATTGTTGGATGTGTTGTTTAGGGTTTTGTTAGTGTTTTTTGAAGATAATGATTAATGATGTATGTAGATTTTTGTAGGTTTTAAGAAGTTGGTTG
++
+BBBFFFFFFBFF<BFFBFFIFBFFFFBBBFBFF<BFFBFFFFFIII7BFBFFIII<FFIIII7BFBFIF<FFBFFFFFF0BB07<<BBFF7<B########
+@HISEQ:105:C2UE1ACXX:3:1101:18680:20134 1:N:0:CAGATC
+TGTTTGGAAGTATGGTGTTTTTTAAGAGGTTTTTGGTTATTTAGTTTGAAATGATTTAGATTATTTTGTTTTTTTGGTTGGGGTAGGTTTTGGAAATTTTT
++
+B<BFFBBFFFFFFFFFFIFFIIIIIBFFFIBFFIB7BFFIIIIFFFI<BFIIBFFIIIBFFIIIIIIBFFFFFFF<7BB70077777BBBF7'<BBFFFFF
+@HISEQ:105:C2UE1ACXX:3:1101:19451:20037 1:N:0:CATATC
+GTTGGTATATTGAGTTTACGTTGCGTTTGTATTATGGAATTTTTTTTGGTGTAGTTTTTGAAGGTGTATTGAGGTTTGAATGAGAGAGGTATTTTTGTTAT
++
+0BB<BBFFBFF0BBFFIIIBFF<B0BFF0BFFFIIB<BFFFIIIIII<7B<BBBFFFFF7<B<BBBBBBF0<70<7<'<BB'<7<'707<<BBFFB07BBF
+@HISEQ:105:C2UE1ACXX:3:1101:14848:20360 1:N:0:CAGATC
+TCTTTATTAATATATAAAAAAAGATATTAGGGAGGATCGAGGGTGTTTTTGTTATGTTTGTTTTATAGAGGAGTATATAGATTGAGGTATAGATACAGGAT
++
+BBBFFFFFFFFFFIIIIIIIIIBFFIIIIBBBFFFFFFBFFFFIIIFFII<BFFFBFFFBFFFFFFF<B<<BBBBBFFF7<BF7<00<<BF0<BFFF007B
+@HISEQ:105:C2UE1ACXX:3:1101:20622:20397 1:N:0:CAAATC
+ATTGTTGTAGTAAAATTTTGAGTTTATAAGTTTTAAAGATTTTAGTAAATTGAGTTTTGTATAGTTTAATATGTATTAAGAATATTTTGGGGATTTTTTTT
++
+BBB<FF<BFBFFFFIIIII0B0BBFFFBFBFFFFFIIBFFFIIF7BFBFFI'00BBFFBBFFF'BFFBBFII0BBB<BB0BBFIIIIF7'''07BB<BBB#
+@HISEQ:105:C2UE1ACXX:3:1101:12295:20565 1:N:0:CAGATC
+ATAAGATAATAAAGTAATAGTTGTGATTAGGAGGTTTTTTATAAGTTGATGGTTTATGTTAAGTAAGTTTATTAAGAAGTATAGTATTATATATAGTATTT
++
+BBBFFFFFFFFFFFFIIIIFII<FBFFIFBBFFFIFFFIIFIIIFFF7BFBBFFFIIBFFIIFFFIFIFFIIIFI<<B7<BFF07<BFFFFFFFF77<BFF
+@HISEQ:105:C2UE1ACXX:3:1101:8987:20904 1:N:0:CAGATC
+TATCGATTTTTTCGGTTATGTGGATTTTTTTTTAGAGGTGATAGTTGTTTTGCGTGTTATCGATGGAGTTTTGGTGGTGGTGGATTGTGTGTTTGGCGTGT
++
+BBBF<BFFFFFFFB<BFFI0<B0BFFIIIIIIIB'<7<F'7BB<BB7BFFF'B0<7<BBFB7BB7'<BBBBB0'7<B<0'7<77BB0<000<<B#######
+@HISEQ:105:C2UE1ACXX:3:1101:11915:21129 1:N:0:CAGATC
+TTTGTTAGTTTTATATTTTTAAGTTTTTTTTTAGGTTCGTTTTAGAAATAGTATGTGTTAAATAGTGAAGTAATTTTTGGTTTTATAATGAGGAGTGGGTA
++
+BBBBFFFFFFFFFIIIIIIIIIFIIIIIIIIIIB7FFF<FFFII<FFIIIBFIFBFFFFFFFFF<BBBFBBFFFFFFF00<BBFFFFFF7<00<0<<<B<B
+@HISEQ:105:C2UE1ACXX:3:1101:12442:21376 1:N:0:CAGATC
+GTGTATATATTAATTATATTTTGATATGTTTGTATAGGACGGTTATTGGAATAAAATATAAAAAAGTAAGTAAGTAAATAAAAAAATATAAGGTGAAAGTT
++
+BBBFFFFFFFFFFIIIIIIIIIBFIII<FFI<FFII<BFFBBFFFII77FFFFIIIIIIIIIIIIBFFFBFFFBFBBFFFFFFFFFFFFFF7077<BB<BB
+@HISEQ:105:C2UE1ACXX:3:1101:10225:21919 1:N:0:CAGATC
+AGTGAAGAGGGAATGAAAAGTTTTTGATTTAATTTATATAATTAAAAAAGTTGGTAGTTATAGTAGTTTAATTTTTTATTATTAAGTTTGTGTGTGGTGTG
++
+BBBFFFFFFFFFFFBFFIIFIFFII<BFFFIFFIIFIIIIIIIIIIIIIFFB77BFBFFFII<FFBFFFIFIFFFFFFFFFFFFF7<BF7B<B<B<7<BB7
+@HISEQ:105:C2UE1ACXX:3:1101:12240:21986 1:N:0:CAGATC
+ATTGTTATTAATATAGGGGGTAGTTTTGGTTTGGGATTGAGGGAGGAATTATAGGAGAATTATTTTTTGTGTAGGAGGGAGAGTTTGAAATTAGGAAGATC
++
+BBBBFFFFFFFFFIIFBFFIIFFIFIIBBFFF77BFFF7B<BFFBFFFFIIFF<<B<BFFFFFFFFFF7BBBFB<<<<<<<BBBBB0BBFFFF00<B7<BB
+@HISEQ:105:C2UE1ACXX:3:1101:2199:22213 1:N:0:CAGATC
+TTAAAGTTATTATTGGAATTTTAAGTTAGGTTATATGTAGGTTATGTATAGGTTTTTATTGTGGGATGAATGGTATTTGGAGCTTGTTTAAAAGTTAGGTT
++
+BBBFFFFFFFFFFIBBFFIIIIFIBFFIBBFFFIFIFIIFFIFFIBFIIIFBFFFIIIIIBFBBFFFBFFF<<FFFFI70<7BFF<BFFFFFFBBBF77<B
+@HISEQ:105:C2UE1ACXX:3:1101:9023:22492 1:N:0:CAGATC
+GTTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGATAGATTGTTTGTTATTGATTTTTTTTGGTAGGACTTTTTATGTTATTTATAGTGAGGAAGAAAGATAT
++
+<BBBFBFFFFFFF<FFFFFFFBFBFFFBBBFFF'''0B0'BBF77B'''''0B0BBFF###########################################
+@HISEQ:105:C2UE1ACXX:3:1101:13060:22287 1:N:0:CAGATC
+TAGTGAAGAATAAATTTTTATGTTGTATATTATTTTTTTTTAGTTCGTATATATCGGTATACGTGTTAGGATTTATAAAGATAGTTATTATTTTTTGTTGT
++
+BBBFFFFFFFFFFIIIIIIIIBFIFFIIIIIIIIIIIIIIII<FFIBFFIIIIIIB<BFFFFBBBBBF<<BBFFFBFFF<BBF7<BFFFFFFFFFF7BB07
+@HISEQ:105:C2UE1ACXX:3:1101:16084:22385 1:N:0:CAGATC
+TATTGAGTTATTGTTTTAGTTTTATGGAAGATTTTTTAAATTGGATTGTTTGTGTTTTTATAGTTTTGAGAGTTTTGTTTGATTTTGTTTTTTTTGTGTTG
++
+BBBF<<BFFFFF0BFFIF<IIIIFFB<FF<FFFFIIIFFFF'0<BFFBFFFBF7FBFIIFIF7BFFI7<7B<BBFB'<BF'7BBFF7BBBBBBB#######
+@HISEQ:105:C2UE1ACXX:3:1101:7272:22581 1:N:0:CAGATC
+TTAATGATATTAAGAATTTTTTAAAGAATTTTATTTTTTTTAGGAATAGAAGGAGGAGGAGTATTTTGATCGATTTTTTAGGTTTTTTATAGGTGGAGTTA
++
+BBBFFBFFFFFFF<BFIIIIIIFIIBFFFFIIFIIIIIIIII<<FFFIBFIBBFFBFFFFFFBBFFF<BBF<BBFFFFFF00<BBFFFFFF000<777B<B
+@HISEQ:105:C2UE1ACXX:3:1101:10060:23020 1:N:0:CAGATC
+TTTTAGATTATTTAAGAAGGTATTAGGTTTTTAAGAGGAAAGGGTAGTCTTATAGTTTTGAGTATTTTTTTTAAAAGGAAGTAAGGATGGTGTTTTTATTT
++
+BBBFFBFFFFFFFIIBFIFFFFFIBBBFFFFIIIBFFFIFIBFFIFFFFIIFFFBFFII7B<FFFIIIIIFFFFBB77<B<BBF<7BB<<BBBBBFFFFFF
+@HISEQ:105:C2UE1ACXX:3:1101:14440:23104 1:N:0:CAGATC
+TTTAAATTTAAGTTAAGGTTTGGGGAGTTGATTTTTGTTTTGTGGGTTGTTTTTTTTGTAGGAGTTGGTTTTTAGAGGTTTTTAGGAATTTTTGGTGTTTT
++
+BBBFFFFFFFFFFFIIBBIFIBBBFFFFFBFFIIIIBFFIIBFBFFFFFFIIIIFFF7BB<<BBFB<BBBBFFB7<00B<BBFF00<BBFFFF''77BBBF
+@HISEQ:105:C2UE1ACXX:3:1101:6941:23338 1:N:0:CAGATC
+AGAAAGGTTTTAAGTTGGTTGGGAATATAGGGGTTTTTTAGAGTTTTTATTAGGAGTTATAGTGTGTTGAATTTGGTTTTGGGTGTTGATTATAGGTTGTT
++
+BBBFFFFFFFFFFBFFBBFFBBBFFFFIIBFFFIFFIIIIBFFFFFIIIIIIB<FFFFFFFBFBFFFF<BBFFF<<BBBB700<BBB0BBFFFF00<B7<B
+@HISEQ:105:C2UE1ACXX:3:1101:10069:23622 1:N:0:CAGATC
+TTTTGTTTTAGGGTTTTATTTTTGTGTTTTATTTTTATTTTCGTATTATTAGTTTTTTTTATACGTTATTTGTAGAAGGTTAGTTTTTTTAATTTAGGTTT
++
+BBBFBFFFFFBBBFFFIFIIIIIBFBFFIIBFIIIIIIIIIIBFFFIIIIIFFIIIIIIFFFFF<BFFFFF<BF<<B77BBB7BBFFFFFFFFFFF00<BB
+@HISEQ:105:C2UE1ACXX:3:1101:14079:24078 1:N:0:CAGATC
+TTTAAAGTTTTTAGTTTTGAGTGGAATTTTAAGAATATTAGTGCGTTTTAAGCTTAGGTAGTTTTGGTAGTTTGAAAGTAATAGGGTGTATTTTGTAAAGT
++
+BBBFFFFFFFFFFBFFFI<BBFFFFFFIIIIIBFFFIIIIBFFFFFFIIIIBFFIIBBFFFFFFI<7BFBFFF<BBF<BBFFF<07BBBBBFFF<BFFF<B
+@HISEQ:105:C2UE1ACXX:3:1101:12064:24631 1:N:0:CCGATC
+TTATAGTGTATTTATATATATGAAATGAATTAATGAATTTTAAAAAAAAAGAAAGTAAGTTGTTTTTAGGATTGATATTTAGAGTTAATTTTTTGAGTTTT
++
+BBBFFBFFFFFFFIIIIIIIIBFIFF0BFIIIIIBFFIIIIIIIIIIIIIBFIIFFFIBFFBFFFFFFB<BFF7BBFFFFF<B<BBBFFFFFFF'70<<BF
+@HISEQ:105:C2UE1ACXX:3:1101:6662:24968 1:N:0:CAGATC
+AGGTGTCGTTTAATTGTTTAGGTTTATGGTATTGTGTTTCGTTTTTTTGGTATTTGTGAGGGTAGAATTGTTTTTGGGTTTTAATTTTTTTAAGTATGGGA
++
+BBBFFFFFFFFFFIIBFFIFBBFFFFIB<FFFI<FBFFFFBFFIIIII77BFFIIBFBFBBBFBBFFFF<BBFFF'07BBBBFFFFFFFFFFB0<BF####
+@HISEQ:105:C2UE1ACXX:3:1101:11630:24964 1:N:0:TAGATC
+TTAGTTTTTTTAGTGTTTTTTATTTATTTCGTTTTATTATTGGAGTTTGTTAAGAAAATTAGGGTTTGATTTGGATGTTAAGGATTGGTTTTTTTTTTGAT
++
+BBBBFFFFFFFFBFFFFIIIIIIIIIIIIIBFFFIFIIIIIB7BFFFFBFFIIBFIIIIIFB<BFBFBFFFF<7BB<BBFF<7<BB77BBFFFFFFFF0<B
+@HISEQ:105:C2UE1ACXX:3:1101:12594:24878 1:N:0:CCGATC
+TTTAATAGGATATGATATTATTTAATTTATAGATTATGGAAATTTTTTATATTTAATGAAGAAAGTTGGAATGTTTTGGGAGGTGTTTAGAATAAATAAAT
++
+BBBFFFFB0BFFF<FFIIIFIIFIIIFIFIF<BFFFF<<BFFFIIIIIIIIIIIIIIBFFBFFIBFB7'<FF<<BBF0''77<BFB<BB0<BBBBBFFBBF
+@HISEQ:105:C2UE1ACXX:3:1101:4483:25030 1:N:0:CAGATC
+AGGATGGTGTTTTTATTTTTAGATTTATATTATTTTGTTATATTTGTATTTGAGTAAGTTTATGGGTTTTTTAAAGAGGTAGGAGGAAGTTTTTTGTTATT
++
+B<BFFFFFFFFFFIIIIIIIIBBFIIFIIIIFIIII<BFFIFFIIBFIIII<BBFFIFIFIIIB7<FFFIIIFBF<<77BB00<70<B7BBBFFF0BBFFF
+@HISEQ:105:C2UE1ACXX:3:1101:12198:25235 1:N:0:CAGATC
+ATATATGTAGTTTGTATTATTTTTGTTATAGTATATAAAGGTTAAAGAGTAGTTGTTTTAATTTTAGAGGTGGAGATTGGGTTGTATAGTTTTGGTTTTTA
++
+BBBFFFBFFFFFFBFFIIFIIIII0BF<FF0BFFFIFFIBBFBFII<FBFBBFF7FFFIIIIIIII<F7<B'<BB7<B''0<<0<BBB77<BB'0<<BBFB
+@HISEQ:105:C2UE1ACXX:3:1101:20477:25084 1:N:0:CATATC
+AGAGTTTATTGAGAAGTAAAGTATTAATTTTATGGGAGAAATGGGATAGAGGTAGTAGAAGTTGTTATGGAATGGGATTAATTAGGAAGTTAATTAAGTGT
++
+BBBFFFFFFF0BBFFBFFIIFFIIIIIIIIIFIBB7FFFFIIFFBFFIFIBFFFFIIFIIFFFBFFFF77BFF<<B<FFFFFFF70<B7BBFFFFFF7B<B
+@HISEQ:105:C2UE1ACXX:3:1101:5725:25359 1:N:0:CAGATC
+GAGAAATAAGATAATAAAGTAATAGTTGTGATTAGGAGGTTTTTTATAAGTTGATGGTTTATGTTAAGTAAGTTTATTAAGAAGTATAGTATTATATATAG
++
+BBBFFFFFFBFFFIIIIIIIIIIIBIFBFFFFIIBBFFFFFFFIIIIIIFFFBFFBBFFFIIBFFIIBFIIFIFIFFFFF<BF<BBFF7<<FFFFFFFFF0
+@HISEQ:105:C2UE1ACXX:3:1101:5502:25591 1:N:0:CAGATC
+ATATGATTTTATTTTTAGGGATAATATTTTTTAAGTGAATTTTGATTTTTTGGTTAGTTATTTTGATGATGTGTAGAGGGTGTATAGTTTTTGGATATAGA
++
+BBBFBFFFFFFFFIIIIBBBFFFIIIIIIIIIIIBFFFIIIIIBFFIIIII<7BFFBFFFIIII<FF<FFBFFFFFB<<<BFBBBF<<BFFF00<BFBF7<
Binary file test-data/t_R1.fastq.gz has changed
Binary file test-data/t_R2.fastq.gz has changed
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/umi-tools_dedup.xml	Wed Jan 10 19:09:42 2018 -0500
@@ -0,0 +1,292 @@
+<tool id="umi_tools_dedup" name="UMI-tools deduplicate" version="@VERSION@.0">
+    <description>Extract UMI from fastq files</description>
+    <macros>
+        <import>macros.xml</import>
+    </macros>
+    <expand macro="requirements">
+        <requirement type="package" version="1.6">samtools</requirement>
+    </expand>
+    <command detect_errors="exit_code"><![CDATA[
+        #if $input.is_of_type("sam"):
+            #set $input_file = $input
+        #else:
+            ln -sf '${input}' 'input.bam' &&
+            ln -sf '$input.metadata.bam_index' 'input.bam.bai' &&
+            #set $input_file = 'input.bam'
+        #end if
+
+        umi_tools dedup
+            --random-seed 0
+            --extract-umi-method $extract_umi_method
+            #if str($extract_umi_method) != 'read_id':
+                --umi-separator '$umi_separator' --umi-tag '$umi_tag'
+            #end if
+            --method $method --edit-distance-threshold $edit_distance_threshold
+            $paired $spliced_is_unique --soft-clip-threshold $soft_clip_threshold
+            $read_length $whole_contig --subset $subset $per_contig $per_gene
+            #if $gene_transcript_map:
+                --gene-transcript-map '$gene_transcript_map'
+            #end if
+            #if len(str($gene_tag)) > 0:
+                --gene-tag '$gene_tag'
+            #end if
+            #if $input.is_of_type("sam"):
+                --in-sam
+            #end if
+            -I '$input_file' -S deduped.bam &&
+            samtools sort deduped.bam -@ \${GALAXY_SLOTS:-1} -o '$output' -O BAM
+    ]]></command>
+    <inputs>
+        <param name="input" type="data" format="sam,bam" label="Reads to deduplicate in SAM or BAM format" />
+        <param name="extract_umi_method" argument="--extract-umi-method" type="select">
+            <option value="read_id" selected="True">Read ID</option>
+            <option value="tag">Tag</option>
+        </param>
+        <param name="umi_separator" argument="--umi-separator" type="text" label="Separator between read id and UMI." help="Ignored unless extracting by tag" />
+        <param name="umi_tag" argument="--umi-tag" type="text" label="Tag which contains UMI." />
+        <param argument="--method" type="select" label="Method used to identify PCR duplicates within reads." help="All methods start by identifying the reads with the same mapping position">
+            <option value="unique">Reads group share the exact same UMI</option>
+            <option value="percentile">Reads group share the exact same UMI. UMIs with counts less than 1% of the median counts for UMIs at the same position are ignored</option>
+            <option value="cluster">Identify clusters based on hamming distance</option>
+            <option value="adjacency">Identify clusters based on hamming distance and resolve networks by using the node counts</option>
+            <option value="directional">Identify clusters based on distance and counts, restrict network expansion by threshold</option>
+        </param>
+        <param name="edit_distance_threshold" argument="--edit-distance-threshold" type="integer" value="1" label="Edit distance threshold" help="For the adjacency and cluster methods the threshold for the edit distance to connect two UMIs in the network can be increased. The default value of 1 works best unless the UMI is very long (&gt;14bp)" />
+        <param argument="--paired" type="boolean" truevalue="--paired" falsevalue="" label="BAM is paired end" help="This will also force the use of the template length to determine reads with the same mapping coordinates." />
+        <param name="spliced_is_unique" argument="--spliced-is-unique" type="boolean" truevalue="--spliced-is-unique" falsevalue="" label="Spliced reads are unique" help="Causes two reads that start in the same position on the same strand and having the same UMI to be considered unique if one is spliced and the other is not. (Uses the 'N' cigar operation to test for splicing)" />
+        <param name="soft_clip_threshold" argument="--soft-clip-threshold" type="integer" value="4" label="Soft clip threshold" help="Mappers that soft clip, will sometimes do so rather than mapping a spliced read if there is only a small overhang over the exon junction. By setting this option, you can treat reads with at least this many bases soft-clipped at the 3' end as spliced." />
+        <param name="read_length" argument="--read-length" type="boolean" truevalue="--read-length" falsevalue="" label="Use the read length as as a criterion when deduping" />
+        <param name="whole_contig" argument="--whole-contig" type="boolean" truevalue="--whole-contig" falsevalue="" label="Consider all alignments to a single contig together" help="This is useful if you have aligned to a transcriptome multi-fasta" />
+        <param argument="--subset" type="float" min="0.0" max="1.0" value="1.0" label="Only consider a random selection of the reads" />
+        <param argument="--chrom" type="boolean" truevalue="--chrom" falsevalue="" label="Only consider a single chromosome" />
+        <param name="per_contig" argument="--per-contig" type="boolean" truevalue="--per-contig" falsevalue="" label="Deduplicate per contig" help="Field 3 in BAM; RNAME. All reads with the same contig will be considered to have the same alignment position. This is useful if your library prep generates PCR duplicates with non identical alignment positions such as CEL-Seq. In this case, you would align to a reference transcriptome with one transcript per gene" />
+        <param name="per_gene" argument="--per-gene" type="boolean" truevalue="--per-gene" falsevalue="" label="Deduplicate per gene" help="As above except with this option you can align to a reference transcriptome with more than one transcript per gene. You need to also provide a map of genes to transcripts. This will also add a metacontig ('MC') tag to the output BAM file." />
+        <param name="gene_transcript_map" argument="--gene-transcript-map" type="data" format="tabular" optional="True" label="Tabular file mapping genes to transripts" />
+        <param name="gene_tag" argument="--gene-tag" type="text" optional="True" label="Deduplicate by this gene tag" help="As --per-gene except here the gene information is encoded in the bam read tag specified so you do not need to supply the mapping file." />
+    </inputs>
+    <outputs>
+        <data format="bam" name="output" />
+    </outputs>
+    <tests>
+        <test>
+            <param name="input" value="group_in1.sam" ftype="sam" />
+            <param name="extract_umi_method" value="read_id" />
+            <param name="method" value="unique" />
+            <output name="output" file="dedup_out1.bam" ftype="bam" sort="True"/>
+        </test>
+        <test>
+            <param name="input" value="group_in2.bam" ftype="bam" />
+            <param name="extract_umi_method" value="read_id" />
+            <param name="paired" value="True" />
+            <param name="method" value="unique" />
+            <output name="output" file="dedup_out2.bam" ftype="bam" sort="True" />
+        </test>
+        <test>
+            <param name="input" value="group_in3.bam" ftype="bam" />
+            <param name="extract_umi_method" value="read_id" />
+            <param name="method" value="unique" />
+            <output name="output" file="dedup_out3.bam" ftype="bam" sort="True" />
+        </test>
+        <test>
+            <param name="input" value="group_in4.bam" ftype="bam" />
+            <param name="extract_umi_method" value="tag" />
+            <param name="umi_tag" value="BX" />
+            <param name="method" value="unique" />
+            <output name="output" file="dedup_out4.bam" ftype="bam" sort="True" />
+        </test>
+        <test>
+            <param name="input" value="group_in5.bam" ftype="bam" />
+            <param name="extract_umi_method" value="read_id" />
+            <param name="umi_tag" value="BX" />
+            <param name="method" value="cluster" />
+            <output name="output" file="dedup_out5.bam" ftype="bam" sort="True" />
+        </test>
+        <test>
+            <param name="input" value="group_in6.bam" ftype="bam" />
+            <param name="extract_umi_method" value="read_id" />
+            <param name="umi_tag" value="BX" />
+            <param name="method" value="directional" />
+            <output name="output" file="dedup_out6.bam" ftype="bam" sort="True" />
+        </test>
+    </tests>
+    <help><![CDATA[
+umi_tools dedup - Deduplicate reads based on their UMI
+======================================================
+
+Purpose
+-------
+
+The purpose of this command is to deduplicate BAM files based on the first
+mapping co-ordinate and the UMI attached to the read. It is assumed that the
+FASTQ files were processed with extract_umi.py before mapping and thus the UMI
+is the last word of the read name. e.g:
+
+@HISEQ:87:00000000_AATT
+
+where AATT is the UMI sequeuence.
+
+If you have used an alternative method which does not separate the
+read id and UMI with a "_", such as bcl2fastq which uses ":", you can
+specify the separator with the option "--umi-separator=<sep>",
+replacing <sep> with e.g ":".
+
+Alternatively, if your UMIs are encoded in a tag, you can specify this
+by setting the option --extract-umi-method=tag and set the tag name
+with the --umi-tag option. For example, if your UMIs are encoded in
+the 'UM' tag, provide the following options:
+"--extract-umi-method=tag --umi-tag=UM"
+
+The start postion of a read is considered to be the start of its alignment
+minus any soft clipped bases. A read aligned at position 500 with
+cigar 2S98M will be assumed to start at postion 498.
+
+
+Methods
+-------
+
+dedup can be run with multiple methods to identify groups of reads with
+the same (or similar) UMI(s). All methods start by identifying the
+reads with the same mapping position.
+
+The simpliest method, "unique", groups reads with the exact same
+UMI. The network-based methods, "cluster", "adjacency" and
+"directional", build networks where nodes are UMIs and edges connect
+UMIs with an edit distance <= threshold (usually 1). The groups of
+reads are then defined from the network in a method-specific manner.
+
+  "unique"
+      Reads group share the exact same UMI
+
+  "percentile"
+      Reads group share the exact same UMI. UMIs with counts < 1% of the
+      median counts for UMIs at the same position are ignored.
+
+  "cluster"
+      Identify clusters of connected UMIs (based on hamming distance
+      threshold). Each network is a read group
+
+  "adjacency"
+      Cluster UMIs as above. For each cluster, select the node(UMI)
+      with the highest counts. Visit all nodes one edge away. If all
+      nodes have been visted, stop. Otherise, repeat with remaining
+      nodes until all nodes have been visted. Each step
+      defines a read group.
+
+  "directional" (default)
+      Identify clusters of connected UMIs (based on hamming distance
+      threshold) and umi A counts >= (2* umi B counts) - 1. Each
+      network is a read group.
+
+Options
+-------
+
+--extract-umi-method (choice)
+      How are the UMIs encoded in the read?
+
+      Options are:
+
+      - "read_id" (default)
+            UMIs contained at the end of the read separated as
+            specified with --umi-separator option
+
+      - "tag"
+            UMIs contained in a tag, see --umi-tag option
+
+--umi-separator (string)
+      Separator between read id and UMI. See --extract-umi-method above
+
+--umi-tag (string)
+      Tag which contains UMI. See --extract-umi-method above
+
+--edit-distance-threshold (int)
+       For the adjacency and cluster methods the threshold for the
+       edit distance to connect two UMIs in the network can be
+       increased. The default value of 1 works best unless the UMI is
+       very long (>14bp)
+
+--paired
+       BAM is paired end - output both read pairs. This will also
+       force the use of the template length to determine reads with
+       the same mapping coordinates.
+
+--spliced-is-unique
+       Causes two reads that start in the same position on the same
+       strand and having the same UMI to be considered unique if one is
+       spliced and the other is not. (Uses the 'N' cigar operation to test
+       for splicing)
+
+--soft-clip-threshold (int)
+       Mappers that soft clip, will sometimes do so rather than mapping a
+       spliced read if there is only a small overhang over the exon
+       junction. By setting this option, you can treat reads with at least
+       this many bases soft-clipped at the 3' end as spliced.
+
+--multimapping-detection-method (string, choice)
+       If the sam/bam contains tags to identify multimapping reads, you can
+       specify for use when selecting the best read at a given loci.
+       Supported tags are "NH", "X0" and "XT". If not specified, the read
+       with the highest mapping quality will be selected
+
+--read-length
+      Use the read length as as a criteria when deduping, for e.g sRNA-Seq
+
+--whole-contig
+      Consider all alignments to a single contig together. This is useful if
+      you have aligned to a transcriptome multi-fasta
+
+--subset (float, [0-1])
+      Only consider a fraction of the reads, chosen at random. This is useful
+      for doing saturation analyses.
+
+--chrom
+      Only consider a single chromosome. This is useful for debugging purposes
+
+--per-contig (string)
+      Deduplicate per contig (field 3 in BAM; RNAME).
+      All reads with the same contig will be
+      considered to have the same alignment position. This is useful
+      if your library prep generates PCR duplicates with non identical
+      alignment positions such as CEL-Seq. In this case, you would
+      align to a reference transcriptome with one transcript per gene
+
+--per-gene (string)
+      Deduplicate per gene. As above except with this option you can
+      align to a reference transcriptome with more than one transcript
+      per gene. You need to also provide --gene-transcript-map option.
+      This will also add a metacontig ('MC') tag to the reads if used
+      in conjunction with --output-bam
+
+--gene-transcript-map (string)
+      File mapping genes to transripts (tab separated), e.g:
+
+      gene1   transcript1
+      gene1   transcript2
+      gene2   transcript3
+
+--gene-tag (string)
+      Deduplicate per gene. As per --per-gene except here the gene
+      information is encoded in the bam read tag specified so you do
+      not need to supply --gene-transcript-map
+
+--output-bam (string, filename)
+      Output a tagged bam file to stdout or -S <filename>
+
+-i, --in-sam/-o, --out-sam
+      By default, inputs are assumed to be in BAM format and output are output
+      in BAM format. Use these options to specify the use of SAM format for
+      inputs or outputs.
+
+-I    (string, filename) input file name
+      The input file must be sorted and indexed.
+
+-S    (string, filename) output file name
+
+-L    (string, filename) log file name
+
+Usage
+-----
+    umi_tools dedup -I infile.bam -S grouped.bam --
+
+    ]]></help>
+    <expand macro="citations" />
+</tool>