Mercurial > repos > iuc > variant_analyzer
diff mut2read.xml @ 2:3f1dbd2c59bf draft default tip
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/variant_analyzer commit f492e9717cb946f0eb5689cd7b6eb8067abf6468"
author | iuc |
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date | Tue, 10 Nov 2020 12:55:29 +0000 |
parents | 3556001ff2db |
children |
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--- a/mut2read.xml Wed Dec 04 16:21:17 2019 -0500 +++ b/mut2read.xml Tue Nov 10 12:55:29 2020 +0000 @@ -1,5 +1,5 @@ <?xml version="1.0" encoding="UTF-8"?> -<tool id="mut2read" name="DCS mutations to tags/reads:" version="1.0.1" profile="19.01"> +<tool id="mut2read" name="DCS mutations to tags/reads:" version="2.0.0" profile="19.01"> <description>Extracts all tags that carry a mutation in the duplex consensus sequence (DCS)</description> <macros> <import>va_macros.xml</import> @@ -17,7 +17,7 @@ ]]> </command> <inputs> - <param name="file1" type="data" format="tabular" label="DCS Mutation File" optional="false" help="TABULAR file with DCS mutations. See Help section below for a detailed explanation."/> + <param name="file1" type="data" format="vcf" label="DCS Mutation File" optional="false" help="VCF file with DCS mutations. See Help section below for a detailed explanation."/> <param name="file2" type="data" format="bam" label="DCS BAM File" optional="false" help="BAM file with aligned DCS reads."/> <param name="file3" type="data" format="tabular" label="Aligned Families File" optional="false" help="TABULAR file with aligned families."/> </inputs> @@ -27,25 +27,25 @@ </outputs> <tests> <test> - <param name="file1" value="DCS_Mutations_test_data_VA.tabular"/> - <param name="file2" value="DCS_test_data_VA.bam"/> - <param name="file3" value="Aligned_Families_test_data_VA.tabular"/> - <output name="output_fastq" file="Interesting_Reads_test_data_VA.fastq" lines_diff="136"/> - <output name="output_json" file="tag_count_dict_test_data_VA.json" lines_diff="2"/> + <param name="file1" value="FreeBayes_test.vcf"/> + <param name="file2" value="DCS_test.bam"/> + <param name="file3" value="Aligned_Families_test.tabular"/> + <output name="output_fastq" file="Interesting_Reads_test.fastq"/> + <output name="output_json" file="tag_count_dict_test.json" lines_diff="2"/> </test> </tests> <help> <![CDATA[ **What it does** -Takes a tabular file with mutations, a BAM file of aligned DCS reads, and a +Takes a VCF file with mutations, a BAM file of aligned DCS reads, and a tabular file with aligned families as input and prints all tags of reads that carry a mutation to a user specified output file and creates a fastq file of reads of tags with a mutation. **Input** -**Dataset 1:** Tabular file with duplex consesus sequence (DCS) mutations as -generated by the **Variant Annotator** tool. +**Dataset 1:** VCF file with duplex consesus sequence (DCS) mutations. E.g. +generated by the `FreeBayes variant caller <https://arxiv.org/abs/1207.3907>`_. **Dataset 2:** BAM file of aligned DCS reads. This file can be obtained by the tool `Map with BWA-MEM <https://arxiv.org/abs/1303.3997>`_.