Mercurial > repos > iuc > variant_analyzer
diff mut2sscs.xml @ 2:3f1dbd2c59bf draft default tip
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/variant_analyzer commit f492e9717cb946f0eb5689cd7b6eb8067abf6468"
author | iuc |
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date | Tue, 10 Nov 2020 12:55:29 +0000 |
parents | 3556001ff2db |
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--- a/mut2sscs.xml Wed Dec 04 16:21:17 2019 -0500 +++ b/mut2sscs.xml Tue Nov 10 12:55:29 2020 +0000 @@ -1,5 +1,5 @@ <?xml version="1.0" encoding="UTF-8"?> -<tool id="mut2sscs" name="DCS mutations to SSCS stats:" version="1.0.1" profile="19.01"> +<tool id="mut2sscs" name="DCS mutations to SSCS stats:" version="2.0.0" profile="19.01"> <description>Extracts all tags from the single stranded consensus sequence (SSCS) bam file that carry a mutation at the same position a mutation is called in the duplex consensus sequence (DCS) and calculates their frequencies</description> <macros> <import>va_macros.xml</import> @@ -15,7 +15,7 @@ ]]> </command> <inputs> - <param name="file1" type="data" format="tabular" label="DCS Mutation File" optional="false" help="TABULAR file with DCS mutations. See Help section below for a detailed explanation."/> + <param name="file1" type="data" format="vcf" label="DCS Mutation File" optional="false" help="VCF file with DCS mutations. See Help section below for a detailed explanation."/> <param name="file2" type="data" format="bam" label="SSCS BAM File" optional="false" help="BAM file with aligned SSCS reads."/> </inputs> <outputs> @@ -23,31 +23,27 @@ </outputs> <tests> <test> - <param name="file1" value="DCS_Mutations_test_data_VA.tabular"/> - <param name="file2" value="SSCS_test_data_VA.bam"/> - <output name="output_json" file="SSCS_counts_test_data_VA.json" lines_diff="2"/> + <param name="file1" value="FreeBayes_test.vcf"/> + <param name="file2" value="SSCS_test.bam"/> + <output name="output_json" file="SSCS_counts_test.json" lines_diff="2"/> </test> </tests> <help> <![CDATA[ **What it does** -Takes a tabular file with DCS mutations and a BAM file of aligned SSCS reads +Takes a VCF file with DCS mutations and a BAM file of aligned SSCS reads as input and writes statistics about tags of reads that carry a mutation in the SSCS at the same position a mutation is called in the DCS to a user specified output file.. **Input** -**Dataset 1:** Tabular file with duplex consesus sequence (DCS) mutations as -generated by the **Variant Annotator** tool. +**Dataset 1:** VCF file with duplex consesus sequence (DCS) mutations. E.g. +generated by the `FreeBayes variant caller <https://arxiv.org/abs/1207.3907>`_. **Dataset 2:** BAM file of aligned single stranded consensus sequence (SSCS) reads. This file can be obtained by the tool `Map with BWA-MEM <https://arxiv.org/abs/1303.3997>`_. -**Dataset 3:** Tabular file with reads as produced by the -**Du Novo: Align families** tool of the `Du Novo Analysis Pipeline -<https://doi.org/10.1186/s13059-016-1039-4>`_ - **Output** The output is a json file containing dictonaries with stats of tags that carry a mutation in the SSCS