annotate varscan_copynumber.xml @ 10:9f8dbbbc0267 draft default tip

"planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 1232893782204bb041dd6ed46711295cb0bfc03f"
author iuc
date Sat, 04 Dec 2021 22:23:36 +0000
parents 4ce3441c407c
children
Ignore whitespace changes - Everywhere: Within whitespace: At end of lines:
rev   line source
2
4ce3441c407c planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 30867f1f022bed18ba1c3b8dc9c54226890b3a9c
iuc
parents: 1
diff changeset
1 <tool id="varscan_copynumber" name="VarScan copynumber" version="@VERSION@.2">
0
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
2 <description>Determine relative tumor copy number from tumor-normal pileups</description>
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
3 <macros>
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
4 <import>macros.xml</import>
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
5 </macros>
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
6 <expand macro="requirements" />
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
7 <expand macro="stdio" />
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
8 <command><![CDATA[
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
9
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
10 varscan copynumber
1
8d8de66233ea planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 33c5694cb2e0a86a76d12a3355a2bd26deba7177
iuc
parents: 0
diff changeset
11 @INPUT_PILEUPS@
0
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
12 --min-coverage ${min_coverage}
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
13 --min-base-qual ${min_base_qual}
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
14 --min-map-qual ${min_map_qual}
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
15
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
16 --min-segment-size ${min_segment_size}
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
17 --max-segment-size ${max_segment_size}
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
18 --p-value ${p_value}
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
19 --data-ratio ${data_ratio}
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
20
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
21 ]]></command>
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
22
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
23 <inputs>
1
8d8de66233ea planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 33c5694cb2e0a86a76d12a3355a2bd26deba7177
iuc
parents: 0
diff changeset
24 <expand macro="input_pileups"/>
0
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
25
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
26 <param argument="--min-coverage" name="min_coverage" type="integer" value="20" min="1" max="200"
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
27 label="Minimum read depth" help="Minimum coverage threshold for copynumber segments"/>
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
28
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
29 <param argument="--min-base-qual" name="min_base_qual" type="integer" value="20" min="1" max="200"
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
30 label="Minimum base quality to count for coverage"/>
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
31 <param argument="--min-map-qual" name="min_map_qual" type="integer" value="20" min="1" max="200"
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
32 label="Minimum read mapping quality to count for coverage"/>
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
33
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
34 <param argument="--min-segment-size" name="min_segment_size" type="integer" value="10" min="1" max="200"
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
35 label="Minimum number of consecutive bases to report a segment" help="" />
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
36 <param argument="--max-segment-size" name="max_segment_size" type="integer" value="100" min="1" max="200"
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
37 label="Max size before a new segment is made" help="" />
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
38
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
39 <expand macro="p_value" label="P-value threshold for significant copynumber change-point" value="0.01" />
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
40
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
41 <param argument="--data-ratio" name="data_ratio" type="float" value="1.0" min="0.0" max="1.0"
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
42 label="The normal/tumor input data ratio for copynumber adjustment"/>
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
43
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
44 </inputs>
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
45 <outputs>
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
46 <data name="output" from_work_dir="galaxy_out.copynumber" format="interval"/>
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
47 </outputs>
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
48 <tests>
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
49 <test>
1
8d8de66233ea planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 33c5694cb2e0a86a76d12a3355a2bd26deba7177
iuc
parents: 0
diff changeset
50 <conditional name="pileup">
8d8de66233ea planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 33c5694cb2e0a86a76d12a3355a2bd26deba7177
iuc
parents: 0
diff changeset
51 <param name="pileup_select" value="separated" />
8d8de66233ea planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 33c5694cb2e0a86a76d12a3355a2bd26deba7177
iuc
parents: 0
diff changeset
52 <param name="normal_pileup" value="N_Region_Chr1_CDKN2C.pileup.gz" />
8d8de66233ea planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 33c5694cb2e0a86a76d12a3355a2bd26deba7177
iuc
parents: 0
diff changeset
53 <param name="tumor_pileup" value="T_Region_Chr1_CDKN2C.pileup.gz" />
8d8de66233ea planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 33c5694cb2e0a86a76d12a3355a2bd26deba7177
iuc
parents: 0
diff changeset
54 </conditional>
0
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
55 <output name="output" file="varscan_copynumber_result1.interval" lines_diff="0" />
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
56 </test>
1
8d8de66233ea planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 33c5694cb2e0a86a76d12a3355a2bd26deba7177
iuc
parents: 0
diff changeset
57 <test>
8d8de66233ea planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 33c5694cb2e0a86a76d12a3355a2bd26deba7177
iuc
parents: 0
diff changeset
58 <conditional name="pileup">
8d8de66233ea planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 33c5694cb2e0a86a76d12a3355a2bd26deba7177
iuc
parents: 0
diff changeset
59 <param name="pileup_select" value="combined" />
8d8de66233ea planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 33c5694cb2e0a86a76d12a3355a2bd26deba7177
iuc
parents: 0
diff changeset
60 <param name="combined_pileup" value="NT.pileup.gz" />
8d8de66233ea planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 33c5694cb2e0a86a76d12a3355a2bd26deba7177
iuc
parents: 0
diff changeset
61 </conditional>
8d8de66233ea planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 33c5694cb2e0a86a76d12a3355a2bd26deba7177
iuc
parents: 0
diff changeset
62 <output name="output" file="varscan_copynumber_result2.interval" lines_diff="0" />
8d8de66233ea planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 33c5694cb2e0a86a76d12a3355a2bd26deba7177
iuc
parents: 0
diff changeset
63 </test>
0
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
64 </tests>
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
65
2
4ce3441c407c planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 30867f1f022bed18ba1c3b8dc9c54226890b3a9c
iuc
parents: 1
diff changeset
66 <help><![CDATA[
4ce3441c407c planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 30867f1f022bed18ba1c3b8dc9c54226890b3a9c
iuc
parents: 1
diff changeset
67 @HELP_HEADER@
0
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
68
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
69 **Input**
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
70
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
71 ::
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
72
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
73 mpileup file - The SAMtools mpileup files for the normal and tumor tissue
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
74
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
75
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
76 **Output**
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
77
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
78 VarScan produces a VCF 4.1 dataset as output.
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
79
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
80
2
4ce3441c407c planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 30867f1f022bed18ba1c3b8dc9c54226890b3a9c
iuc
parents: 1
diff changeset
81 ]]></help>
0
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
82 <expand macro="citations" />
a584f046e4a7 planemo upload for repository https://github.com/galaxyproject/iuc/tree/master/tools/varscan commit 44c3f913e091bfdb94870ec3181390ad98711797
iuc
parents:
diff changeset
83 </tool>