# HG changeset patch
# User iuc
# Date 1611225229 0
# Node ID fb20fa735dfeb3fbb599953a7a5ab592312dc2c7
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
diff -r 000000000000 -r fb20fa735dfe macros.xml
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/macros.xml Thu Jan 21 10:33:49 2021 +0000
@@ -0,0 +1,69 @@
+
+
+ 0.3.2
+ 0
+ 20.01
+
+
+ vcfanno
+ tabix
+ samtools
+
+
+
+ vcfanno --help
+
+
+
+ @online{vcfanno,
+ author = {Brent S. Pedersen},
+ title = {vcfanno},
+ year = 2016,
+ url = {https://github.com/brentp/vcfanno},
+ urldate = {2020-12-03}
+ }
+ 10.1186/s13059-016-0973-5
+
+
+
+
+ vcfanno
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ `_ and `github `_.
+ ]]>
+
diff -r 000000000000 -r fb20fa735dfe test-data/ex.bam
Binary file test-data/ex.bam has changed
diff -r 000000000000 -r fb20fa735dfe test-data/ex.bam.bai
Binary file test-data/ex.bam.bai has changed
diff -r 000000000000 -r fb20fa735dfe test-data/exac.vcf.gz
Binary file test-data/exac.vcf.gz has changed
diff -r 000000000000 -r fb20fa735dfe test-data/fitcons.bed
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/fitcons.bed Thu Jan 21 10:33:49 2021 +0000
@@ -0,0 +1,537 @@
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diff -r 000000000000 -r fb20fa735dfe test-data/query.vcf.gz
Binary file test-data/query.vcf.gz has changed
diff -r 000000000000 -r fb20fa735dfe test-data/vcfanno_result.vcf
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/vcfanno_result.vcf Thu Jan 21 10:33:49 2021 +0000
@@ -0,0 +1,487 @@
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+1 10616 . CCGCCGTTGCAAAGGCGCGCCG C 580.2 . AB=0.4375;ABP=3.55317;AC=31;AF=0.911765;AN=34;AO=54;CIGAR=1M21D1M;TYPE=del;mapq=21;coverage=1;xdp2=0,1 GT:DP:RO:QR:AO:QA:GL 1/1:2:0:0:2:35:-3.325,-0.60206,0 1/1:5:0:0:4:106:-9.744,-1.20412,0 1/1:9:0:0:9:208:-10,-2.70927,0
+1 10623 . TGCAAAGGC CGCAAAG 580.2 . AB=0.375;ABP=5.18177;AC=3;AF=0.0882353;AN=34;AO=10;CIGAR=7M1X6M2D7M;TYPE=complex GT:DP:RO:QR:AO:QA:GL ./.:2:0:0:0:0:-3.325,-3.325,-3.325 ./.:5:0:0:0:0:-9.744,-9.744,-9.744 ./.:9:0:0:0:0:-10,-10,-10
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+1 10671 . G C 215.9 . AB=0.304878;ABP=30.1272;AC=7;AF=0.205882;AN=34;AO=33;CIGAR=1X;TYPE=snp;mapq=21;coverage=1;xdp2=0,1 GT:DP:RO:QR:AO:QA:GL 0/0:9:9:254:0:0:0,-2.70927,-10 0/0:6:6:163:0:0:0,-1.80618,-10 0/1:12:11:273:1:39:-1.36682,0,-10
+1 10683 . G C 244.6 . AB=0.3125;ABP=17.6677;AC=4;AF=0.117647;AN=34;AO=20;CIGAR=1X;TYPE=snp;mapq=21;coverage=1;xdp2=0,1 GT:DP:RO:QR:AO:QA:GL 0/0:8:8:264:0:0:0,-2.40824,-10 0/0:8:8:220:0:0:0,-2.40824,-10 0/0:11:11:324:0:0:0,-3.31133,-10
+1 10693 . C G 281.7 . AB=0.343284;ABP=17.3031;AC=6;AF=0.176471;AN=34;AO=31;CIGAR=1X;TYPE=snp;mapq=21;coverage=1;xdp2=0,1 GT:DP:RO:QR:AO:QA:GL 0/0:8:8:241:0:0:0,-2.40824,-10 0/0:8:8:236:0:0:0,-2.40824,-10 0/0:11:11:359:0:0:0,-3.31133,-10
+1 10694 . C G 1824.5 . AB=0.529412;ABP=4.41537;AC=17;AF=0.5;AN=34;AO=99;CIGAR=1X;TYPE=snp;mapq=21;coverage=1;xdp2=0,1 GT:DP:RO:QR:AO:QA:GL 0/1:8:4:93:4:132:-10,0,-8.03936 0/1:8:3:76:5:131:-10,0,-6.43328 0/1:11:4:131:7:213:-10,0,-10
+1 10700 . G C 365.3 . AB=0.369863;ABP=13.7487;AC=7;AF=0.205882;AN=34;AO=31;CIGAR=1X;TYPE=snp;mapq=21;coverage=1;xdp2=0,1 GT:DP:RO:QR:AO:QA:GL 0/0:8:8:274:0:0:0,-2.40824,-10 0/0:9:9:291:0:0:0,-2.70927,-10 0/0:11:11:368:0:0:0,-3.31133,-10
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+1 10722 . C G 230.0 . AB=0.311475;ABP=21.8416;AC=6;AF=0.176471;AN=34;AO=26;CIGAR=1X;TYPE=snp;mapq=21;coverage=1;xdp2=0,1 GT:DP:RO:QR:AO:QA:GL 0/0:5:5:184:0:0:0,-1.50515,-10 0/0:9:9:314:0:0:0,-2.70927,-10 0/0:10:10:347:0:0:0,-3.0103,-10
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+1 10729 . G C 295.1 . AB=0.367347;ABP=10.4997;AC=6;AF=0.176471;AN=34;AO=21;CIGAR=1X;TYPE=snp;mapq=21;coverage=1;xdp2=0,1 GT:DP:RO:QR:AO:QA:GL 0/0:2:2:73:0:0:0,-0.60206,-6.935 0/0:8:8:270:0:0:0,-2.40824,-10 0/0:7:7:239:0:0:0,-2.10721,-10
+1 10741 . G C 120.7 . AB=0.409091;ABP=4.58955;AC=4;AF=0.125;AN=32;AO=10;CIGAR=1X;TYPE=snp GT:DP:RO:QR:AO:QA:GL .:.:.:.:.:.:. 0/0:6:6:208:0:0:0,-1.80618,-10 0/0:4:4:139:0:0:0,-1.20412,-10
+1 17594 . C T 58.3 . AB=0.235294;ABP=13.3567;AC=1;AF=0.0294118;AN=34;AO=4;CIGAR=1X;TYPE=snp;mapq=40;coverage=7;xdp2=0,7 GT:DP:RO:QR:AO:QA:GL 0/0:26:26:916:0:0:0,-7.82678,-10 0/0:12:12:440:0:0:0,-3.61236,-10 0/0:18:18:610:0:0:0,-5.41854,-10
+1 17614 . G A 234.0 . AB=0.185567;ABP=86.3098;AC=4;AF=0.117647;AN=34;AO=20;CIGAR=1X;TYPE=snp;mapq=35.75;coverage=8;xdp2=0,8 GT:DP:RO:QR:AO:QA:GL 0/0:33:33:1181:0:0:0,-9.93399,-10 0/0:16:16:580:0:0:0,-4.81648,-10 0/0:23:23:811:0:0:0,-6.92369,-10
+1 17626 . G A 70.2 . AB=0.15625;ABP=68.6973;AC=3;AF=0.0882353;AN=34;AO=18;CIGAR=1X;TYPE=snp;mapq=35.1429;coverage=7;xdp2=0,7 GT:DP:RO:QR:AO:QA:GL 0/0:38:38:1359:0:0:0,-10,-10 0/0:20:20:716:0:0:0,-6.0206,-10 0/0:25:25:914:0:0:0,-7.52575,-10
+1 17697 . G C 195.1 . AB=0.164835;ABP=91.802;AC=2;AF=0.0588235;AN=34;AO=15;CIGAR=1X;TYPE=snp GT:DP:RO:QR:AO:QA:GL 0/0:65:65:2215:0:0:0,-10,-10 0/1:46:40:1387:6:236:-10,0,-10 0/0:57:57:1918:0:0:0,-10,-10
+1 17722 . A G 899.0 . AB=0.171429;ABP=265.572;AC=5;AF=0.147059;AN=34;AO=58;CIGAR=1X;TYPE=snp GT:DP:RO:QR:AO:QA:GL 0/0:66:64:2340:2:80:0,-8.93655,-10 0/0:45:45:1703:0:0:0,-10,-10 0/0:63:63:2319:0:0:0,-10,-10
+1 17730 . C A 575.3 . AB=0.166038;ABP=259.728;AC=5;AF=0.147059;AN=34;AO=53;CIGAR=1X;TYPE=snp GT:DP:RO:QR:AO:QA:GL 0/0:68:65:2332:2:48:0,-10,-10 0/0:45:45:1693:0:0:0,-10,-10 0/0:62:62:2277:0:0:0,-10,-10
+1 17746 . A G 3136.5 . AB=0.232172;ABP=378.711;AC=13;AF=0.382353;AN=34;AO=144;CIGAR=1X;TYPE=snp GT:DP:RO:QR:AO:QA:GL 0/1:57:36:1275:21:779:-10,0,-10 0/1:39:32:1187:7:260:-10,0,-10 0/1:55:49:1802:6:224:-10,0,-10
+1 17765 . G A 156.7 . AB=0.222222;ABP=33.1696;AC=1;AF=0.0294118;AN=34;AO=10;CIGAR=1X;TYPE=snp GT:DP:RO:QR:AO:QA:GL 0/1:45:35:1233:10:329:-10,0,-10 0/0:36:36:1320:0:0:0,-10,-10 0/0:51:51:1811:0:0:0,-10,-10
+1 18934 . C T 28.1 . AB=0.368421;ABP=5.8675;AC=5;AF=0.147059;AN=34;AO=9;CIGAR=1X;TYPE=snp GT:DP:RO:QR:AO:QA:GL 0/1:7:5:138:2:65:-5.39001,0,-10 0/0:4:4:152:0:0:0,-1.20412,-10 0/0:5:4:153:1:30:-2.19382,0,-10
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+1 19172 . A G 306.4 . AB=0.298851;ABP=33.5856;AC=10;AF=0.294118;AN=34;AO=31;CIGAR=1X;TYPE=snp;mapq=15;coverage=1;xdp2=0,1 GT:DP:RO:QR:AO:QA:GL 0/0:9:9:328:0:0:0,-2.70927,-10 0/1:4:3:111:1:31:-2.49794,0,-9.75794 0/0:13:12:431:1:34:-0.600553,0,-10
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+1 19322 . C T 683.2 . AB=0.386792;ABP=14.81;AC=12;AF=0.352941;AN=34;AO=43;CIGAR=1X;TYPE=snp;mapq=12;coverage=3;xdp2=2,1 GT:DP:RO:QR:AO:QA:GL 0/0:11:11:355:0:0:0,-3.31133,-10 0/1:7:4:111:3:101:-8.86352,0,-9.70436 0/1:20:12:386:8:211:-10,0,-10
+1 19355 . G A 273.7 . AB=0.5;ABP=3.0103;AC=8;AF=0.25;AN=32;AO=20;CIGAR=1X;TYPE=snp;mapq=16;coverage=3;xdp2=0,3 GT:DP:RO:QR:AO:QA:GL 0/1:7:2:62:5:137:-10,0,-5.10501 0/1:4:2:71:2:53:-4.60903,0,-6.31903 0/1:6:4:131:2:61:-5.16491,0,-10
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+1 19425 . A G 895.3 . AB=0.290456;ABP=94.924;AC=17;AF=0.5;AN=34;AO=70;CIGAR=1X;TYPE=snp;mapq=18;coverage=3;xdp2=0,3 GT:DP:RO:QR:AO:QA:GL 0/1:21:18:514:3:112:-7.25556,0,-10 0/1:13:8:298:5:172:-10,0,-10 0/1:29:23:763:4:57:-1.2948,0,-10
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+1 66376 . TA T 1265.4 . AB=0.223684;ABP=53.4113;AC=2;AF=0.142857;AN=14;AO=17;CIGAR=5M1D2M;TYPE=del GT:DP:RO:QR:AO:QA:GL .:.:.:.:.:.:. .:.:.:.:.:.:. ./1:33:0:0:9:361:-10,-10,-10
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+1 97661 . T C 33.4 . AB=0.3;ABP=6.48466;AC=1;AF=0.0333333;AN=30;AO=5;CIGAR=1X;TYPE=snp GT:DP:RO:QR:AO:QA:GL 0/0:19:18:629:1:41:0,-0.340816,-10 0/0:2:2:74:0:0:0,-0.60206,-7.03 0/0:8:8:292:0:0:0,-2.40824,-10
+1 97937 . T A 12.3 . AB=0.428571;ABP=3.32051;AC=2;AF=0.0588235;AN=34;AO=5;CIGAR=1X;TYPE=snp GT:DP:RO:QR:AO:QA:GL 0/0:26:26:899:0:0:0,-7.82678,-10 0/0:3:3:113:0:0:0,-0.90309,-10 0/0:7:7:248:0:0:0,-2.10721,-10
+1 97953 . A G 1000.6 . AB=0.401709;ABP=12.8283;AC=13;AF=0.382353;AN=34;AO=48;CIGAR=1X;TYPE=snp;mapq=20.5;coverage=2;xdp2=2,0 GT:DP:RO:QR:AO:QA:GL 0/0:32:32:1102:0:0:0,-9.63296,-10 0/1:6:4:155:2:77:-6.68491,0,-10 0/0:10:10:355:0:0:0,-3.0103,-10
+1 98683 . G A 610.5 . AB=0.282443;ABP=56.8661;AC=11;AF=0.34375;AN=32;AO=45;CIGAR=1X;TYPE=snp;AC_AFR=0;AC_AMR=0;AC_EAS=0 GT:DP:RO:QR:AO:QA:GL 0/0:73:71:2641:2:78:0,-10,-10 0/0:1:0:0:1:34:-3.4,-0.30103,0 0/1:33:29:1059:4:139:-7.53545,0,-10
+1 98688 . G 610.5 . CIEND=-4,2;SVLEN=10;AB=0.282443;ABP=56.8661;AC=11;AF=0.34375;AN=32;AO=45;CIGAR=1X;TYPE=snp GT:DP:RO:QR:AO:QA:GL 0/0:73:71:2641:2:78:0,-10,-10 0/0:1:0:0:1:34:-3.4,-0.30103,0 0/1:33:29:1059:4:139:-7.53545,0,-10
+2 98688 . G 610.5 . CIEND=-4,2;SVLEN=10;AB=0.282443;ABP=56.8661;AC=11;AF=0.34375;AN=32;AO=45;CIGAR=1X;TYPE=snp GT:DP:RO:QR:AO:QA:GL 0/0:73:71:2641:2:78:0,-10,-10 0/0:1:0:0:1:34:-3.4,-0.30103,0 0/1:33:29:1059:4:139:-7.53545,0,-10
diff -r 000000000000 -r fb20fa735dfe vcfanno.xml
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/vcfanno.xml Thu Jan 21 10:33:49 2021 +0000
@@ -0,0 +1,266 @@
+
+
+ VCF annotation
+
+ macros.xml
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+ '$out_vcf'
+ ]]>
+
+ #
+#for $i, $annotation in enumerate($annotations):
+ #set $ext = $annotation.type.type_select
+ #if $ext == 'vcf' or $ext == 'bed':
+ #set $ext = $ext + ".gz"
+ #end if
+ #if $ext == 'vcf.gz' or $ext == 'bam':
+ #set $annotation_names = list()
+ #set $annotation_fields = list()
+ #set $annotation_ops = list()
+ #for $field in $annotation.type.fields:
+ #silent $annotation_names.append('"%s"' %(str($field.name)))
+ #silent $annotation_fields.append('"%s"' %(str($field.field)))
+ #silent $annotation_ops.append('"%s"' %(str($field.op)))
+ #end for
+ #set $names = "names= [%s]" % (", ".join($annotation_names))
+ #set $fields = "fields= [%s]" % (", ".join($annotation_fields))
+ #set $ops = "ops= [%s]" % (", ".join($annotation_ops))
+ #elif $ext == 'bed.gz':
+ #set $annotation_names = list()
+ #set $annotation_columns = list()
+ #set $annotation_ops = list()
+ #for $field in $annotation.type.fields:
+ #silent $annotation_names.append('"%s"' %(str($field.name)))
+ #silent $annotation_columns.append( str($field.columns))
+ #silent $annotation_ops.append('"%s"' %(str($field.op)))
+ #end for
+ #set $names = "names= [%s]" % (", ".join($annotation_names))
+ #set $fields = "columns= [%s]" % (", ".join($annotation_columns))
+ #set $ops = "ops= [%s]" % (", ".join($annotation_ops))
+ #end if
+[[annotation]]
+file="input_${i}.${ext}"
+${names}
+${fields}
+${ops}
+
+#end for
+
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