diff test-data/day_18.vcf @ 0:d2a1ac520392 draft

planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/virheat commit ed302b5ddedadffc87798b7216f1a331accce96e
author iuc
date Wed, 15 May 2024 13:37:49 +0000
parents
children
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--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/day_18.vcf	Wed May 15 13:37:49 2024 +0000
@@ -0,0 +1,113 @@
+##fileformat=VCFv4.0
+##fileDate=20220830
+##source=lofreq call --verbose --ref reference.fa --call-indels --min-cov 20 --max-depth 1000000 --min-bq 30 --min-alt-bq 30 --min-mq 20 --max-mq 255 --min-jq 0 --min-alt-jq 0 --def-alt-jq 0 --sig 0.001 --bonf dynamic --no-default-filter --no-default-filter -r NC_045512:1-7475 -o /data/jwd/main/050/063/50063644/tmp/lofreq2_call_parallel717nv31u/0.vcf.gz reads.bam 
+##reference=reference.fa
+##INFO=<ID=DP,Number=1,Type=Integer,Description="Raw Depth">
+##INFO=<ID=AF,Number=1,Type=Float,Description="Allele Frequency">
+##INFO=<ID=SB,Number=1,Type=Integer,Description="Phred-scaled strand bias at this position">
+##INFO=<ID=DP4,Number=4,Type=Integer,Description="Counts for ref-forward bases, ref-reverse, alt-forward and alt-reverse bases">
+##INFO=<ID=INDEL,Number=0,Type=Flag,Description="Indicates that the variant is an INDEL.">
+##INFO=<ID=CONSVAR,Number=0,Type=Flag,Description="Indicates that the variant is a consensus variant (as opposed to a low frequency variant).">
+##INFO=<ID=HRUN,Number=1,Type=Integer,Description="Homopolymer length to the right of report indel position">
+##FILTER=<ID=min_snvqual_62,Description="Minimum SNV Quality (Phred) 62">
+##FILTER=<ID=min_indelqual_48,Description="Minimum Indel Quality (Phred) 48">
+##FILTER=<ID=min_snvqual_71,Description="Minimum SNV Quality (Phred) 71">
+##FILTER=<ID=min_indelqual_57,Description="Minimum Indel Quality (Phred) 57">
+##FILTER=<ID=min_af_0.100000,Description="Minimum allele frequency 0.100000">
+##FILTER=<ID=min_dp_20,Description="Minimum Coverage 20">
+##FILTER=<ID=sb_fdr,Description="Strand-Bias Multiple Testing Correction: fdr corr. pvalue > 0.001000">
+##SnpEffVersion="4.3t (build 2017-11-24 10:18), by Pablo Cingolani"
+##SnpEffCmd="SnpEff  -i vcf -o vcf -formatEff -classic -no-downstream -no-intergenic -no-intron -no-upstream -stats /data/jwd/main/050/063/50063750/outputs/galaxy_dataset_232ae126-f2f4-472c-bf70-443ea6a4b576.dat -csvStats /data/jwd/main/050/063/50063750/outputs/galaxy_dataset_801f99b1-8ef7-4504-b4e3-a04f423e6349.dat SARS-CoV-2 /data/dnb07/galaxy_db/files/a/3/d/dataset_a3de6b1f-173b-4ce7-85a4-51a14281eafa.dat "
+##INFO=<ID=EFF,Number=.,Type=String,Description="Predicted effects for this variant.Format: 'Effect ( Effect_Impact | Functional_Class | Codon_Change | Amino_Acid_Change| Amino_Acid_length | Gene_Name | Transcript_BioType | Gene_Coding | Transcript_ID | Exon_Rank  | Genotype [ | ERRORS | WARNINGS ] )' ">
+##INFO=<ID=LOF,Number=.,Type=String,Description="Predicted loss of function effects for this variant. Format: 'Gene_Name | Gene_ID | Number_of_transcripts_in_gene | Percent_of_transcripts_affected'">
+##INFO=<ID=NMD,Number=.,Type=String,Description="Predicted nonsense mediated decay effects for this variant. Format: 'Gene_Name | Gene_ID | Number_of_transcripts_in_gene | Percent_of_transcripts_affected'">
+#CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO
+NC_045512	509	.	GGTCATGTTA	G	26636.0	PASS	AF=0.998797;DP=831;DP4=20,27,616,214;HRUN=2;SB=49;INDEL;EFF=CODON_CHANGE_PLUS_CODON_DELETION(MODERATE||ggtcatgttatg/gtg|GHVM82V|7101|ORF1ab|protein_coding|CODING|GU280_gp01|1|G)
+NC_045512	670	.	T	G	18624.0	PASS	AF=0.951128;DP=532;DP4=0,0,250,281;SB=0;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|agT/agG|S135R|7101|ORF1ab|protein_coding|CODING|GU280_gp01|1|G)
+NC_045512	900	.	C	T	49314.0	PASS	AF=0.969347;DP=1990;DP4=1,0,1026,960;SB=0;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|tCa/tTa|S212L|7101|ORF1ab|protein_coding|CODING|GU280_gp01|1|T)
+NC_045512	1980	.	G	T	4529.0	PASS	AF=0.967742;DP=124;DP4=0,0,67,57;SB=0;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gGa/gTa|G572V|7101|ORF1ab|protein_coding|CODING|GU280_gp01|1|T)
+NC_045512	2790	.	C	T	8006.0	PASS	AF=0.969163;DP=227;DP4=0,0,116,111;SB=0;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aCt/aTt|T842I|7101|ORF1ab|protein_coding|CODING|GU280_gp01|1|T)
+NC_045512	3037	.	C	T	28216.0	PASS	AF=0.962963;DP=783;DP4=0,1,361,419;SB=0;EFF=SYNONYMOUS_CODING(LOW|SILENT|ttC/ttT|F924|7101|ORF1ab|protein_coding|CODING|GU280_gp01|1|T)
+NC_045512	4184	.	G	A	9244.0	PASS	AF=0.968627;DP=255;DP4=0,0,174,81;SB=0;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ggt/Agt|G1307S|7101|ORF1ab|protein_coding|CODING|GU280_gp01|1|A)
+NC_045512	4276	.	C	T	7858.0	PASS	AF=0.981132;DP=212;DP4=0,0,173,39;SB=0;EFF=SYNONYMOUS_CODING(LOW|SILENT|taC/taT|Y1337|7101|ORF1ab|protein_coding|CODING|GU280_gp01|1|T)
+NC_045512	4321	.	C	T	1600.0	PASS	AF=0.460870;DP=115;DP4=61,0,27,27;SB=108;EFF=SYNONYMOUS_CODING(LOW|SILENT|gcC/gcT|A1352|7101|ORF1ab|protein_coding|CODING|GU280_gp01|1|T)
+NC_045512	6354	.	C	T	4133.0	PASS	AF=0.774834;DP=151;DP4=29,1,80,41;SB=33;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|tCa/tTa|S2030L|7101|ORF1ab|protein_coding|CODING|GU280_gp01|1|T)
+NC_045512	9344	.	C	T	23951.0	PASS	AF=0.977169;DP=657;DP4=1,0,317,339;SB=3;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ctt/Ttt|L3027F|7101|ORF1ab|protein_coding|CODING|GU280_gp01|1|T)
+NC_045512	9424	.	A	G	21689.0	PASS	AF=0.959217;DP=613;DP4=0,0,318,294;SB=0;EFF=SYNONYMOUS_CODING(LOW|SILENT|gtA/gtG|V3053|7101|ORF1ab|protein_coding|CODING|GU280_gp01|1|G)
+NC_045512	9534	.	C	T	49314.0	PASS	AF=0.974796;DP=1349;DP4=0,0,545,804;SB=0;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aCt/aTt|T3090I|7101|ORF1ab|protein_coding|CODING|GU280_gp01|1|T)
+NC_045512	9866	.	C	T	23400.0	PASS	AF=0.959815;DP=647;DP4=0,0,481,166;SB=0;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ctt/Ttt|L3201F|7101|ORF1ab|protein_coding|CODING|GU280_gp01|1|T)
+NC_045512	10029	.	C	T	23958.0	PASS	AF=0.933430;DP=691;DP4=0,0,283,405;SB=0;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aCc/aTc|T3255I|7101|ORF1ab|protein_coding|CODING|GU280_gp01|1|T)
+NC_045512	10198	.	C	T	17239.0	PASS	AF=0.942623;DP=488;DP4=0,0,252,235;SB=0;EFF=SYNONYMOUS_CODING(LOW|SILENT|gaC/gaT|D3311|7101|ORF1ab|protein_coding|CODING|GU280_gp01|1|T)
+NC_045512	10447	.	G	A	12177.0	PASS	AF=0.942197;DP=346;DP4=0,1,178,167;SB=3;EFF=SYNONYMOUS_CODING(LOW|SILENT|agG/agA|R3394|7101|ORF1ab|protein_coding|CODING|GU280_gp01|1|A)
+NC_045512	10449	.	C	A	11904.0	PASS	AF=0.924638;DP=345;DP4=0,1,177,166;SB=3;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cCc/cAc|P3395H|7101|ORF1ab|protein_coding|CODING|GU280_gp01|1|A)
+NC_045512	11287	.	GTCTGGTTTT	G	3147.0	PASS	AF=1.000000;DP=96;DP4=5,12,39,57;HRUN=1;SB=3;INDEL;EFF=CODON_DELETION(MODERATE||tctggtttt/|SGF3675|7101|ORF1ab|protein_coding|CODING|GU280_gp01|1|G)
+NC_045512	12036	.	C	T	19886.0	PASS	AF=0.951473;DP=577;DP4=0,1,262,313;SB=0;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gCt/gTt|A3924V|7101|ORF1ab|protein_coding|CODING|GU280_gp01|1|T)
+NC_045512	12854	.	C	T	13932.0	PASS	AF=0.968750;DP=384;DP4=1,1,181,201;SB=0;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cct/Tct|P4197S|7101|ORF1ab|protein_coding|CODING|GU280_gp01|1|T)
+NC_045512	12880	.	C	T	13710.0	PASS	AF=0.973545;DP=378;DP4=0,0,171,207;SB=0;EFF=SYNONYMOUS_CODING(LOW|SILENT|atC/atT|I4205|7101|ORF1ab|protein_coding|CODING|GU280_gp01|1|T)
+NC_045512	14408	.	C	T	49314.0	PASS	AF=0.966997;DP=1515;DP4=0,0,790,723;SB=0;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cCt/cTt|P4720L|7101|ORF1ab|protein_coding|CODING|GU280_gp01|2|T)
+NC_045512	15451	.	G	A	36998.0	PASS	AF=0.948989;DP=1039;DP4=0,0,768,270;SB=0;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ggt/Agt|G5068S|7101|ORF1ab|protein_coding|CODING|GU280_gp01|2|A)
+NC_045512	15714	.	C	T	23967.0	PASS	AF=0.978626;DP=655;DP4=0,0,327,328;SB=0;EFF=SYNONYMOUS_CODING(LOW|SILENT|ctC/ctT|L5155|7101|ORF1ab|protein_coding|CODING|GU280_gp01|2|T)
+NC_045512	17259	.	G	T	671.0	PASS	AF=0.217742;DP=124;DP4=51,45,25,3;SB=34;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gaG/gaT|E5670D|7101|ORF1ab|protein_coding|CODING|GU280_gp01|2|T)
+NC_045512	17410	.	C	T	3135.0	PASS	AF=0.977273;DP=88;DP4=0,0,39,49;SB=0;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cgt/Tgt|R5721C|7101|ORF1ab|protein_coding|CODING|GU280_gp01|2|T)
+NC_045512	18163	.	A	G	6626.0	PASS	AF=0.978261;DP=184;DP4=0,0,87,97;SB=0;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ata/Gta|I5972V|7101|ORF1ab|protein_coding|CODING|GU280_gp01|2|G)
+NC_045512	19955	.	C	T	48074.0	PASS	AF=0.976209;DP=1303;DP4=0,0,917,385;SB=0;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aCt/aTt|T6569I|7101|ORF1ab|protein_coding|CODING|GU280_gp01|2|T)
+NC_045512	20055	.	A	G	13443.0	PASS	AF=0.938303;DP=389;DP4=14,1,179,195;SB=34;EFF=SYNONYMOUS_CODING(LOW|SILENT|gaA/gaG|E6602|7101|ORF1ab|protein_coding|CODING|GU280_gp01|2|G)
+NC_045512	20719	.	C	T	15386.0	PASS	AF=0.965035;DP=429;DP4=0,0,235,194;SB=0;EFF=SYNONYMOUS_CODING(LOW|SILENT|Cta/Tta|L6824|7101|ORF1ab|protein_coding|CODING|GU280_gp01|2|T)
+NC_045512	21618	.	C	T	49314.0	PASS	AF=0.987821;DP=1478;DP4=1,0,719,758;SB=3;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aCa/aTa|T19I|1273|S|protein_coding|CODING|GU280_gp02|1|T)
+NC_045512	21632	.	TTACCCCCTG	T	47313.0	PASS	AF=0.994092;DP=1354;DP4=32,30,649,697;HRUN=2;SB=2;INDEL;EFF=CODON_CHANGE_PLUS_CODON_DELETION(MODERATE||ttaccccctgca/tca|LPPA24S|1273|S|protein_coding|CODING|GU280_gp02|1|T)
+NC_045512	21987	.	G	A	34703.0	PASS	AF=0.945697;DP=976;DP4=2,1,451,520;SB=2;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gGt/gAt|G142D|1273|S|protein_coding|CODING|GU280_gp02|1|A)
+NC_045512	22200	.	T	G	6387.0	PASS	AF=0.967742;DP=186;DP4=0,0,93,93;SB=0;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gTg/gGg|V213G|1273|S|protein_coding|CODING|GU280_gp02|1|G)
+NC_045512	22578	.	G	A	21408.0	PASS	AF=0.965347;DP=606;DP4=0,0,314,291;SB=0;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gGt/gAt|G339D|1273|S|protein_coding|CODING|GU280_gp02|1|A)
+NC_045512	22629	.	A	C	19252.0	PASS	AF=0.964750;DP=539;DP4=1,1,290,247;SB=0;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aAg/aCg|K356T|1273|S|protein_coding|CODING|GU280_gp02|1|C)
+NC_045512	22664	.	C	A	19308.0	PASS	AF=0.977570;DP=535;DP4=0,0,277,256;SB=0;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cta/Ata|L368I|1273|S|protein_coding|CODING|GU280_gp02|1|A)
+NC_045512	22674	.	C	T	18944.0	PASS	AF=0.964618;DP=537;DP4=0,0,282,255;SB=0;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|tCc/tTc|S371F|1273|S|protein_coding|CODING|GU280_gp02|1|T)
+NC_045512	22679	.	T	C	18999.0	PASS	AF=0.964684;DP=538;DP4=0,0,287,251;SB=0;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Tca/Cca|S373P|1273|S|protein_coding|CODING|GU280_gp02|1|C)
+NC_045512	22686	.	C	T	17680.0	PASS	AF=0.964497;DP=507;DP4=1,0,271,235;SB=0;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|tCc/tTc|S375F|1273|S|protein_coding|CODING|GU280_gp02|1|T)
+NC_045512	22688	.	A	G	17586.0	PASS	AF=0.950000;DP=500;DP4=1,0,268,228;SB=0;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Act/Gct|T376A|1273|S|protein_coding|CODING|GU280_gp02|1|G)
+NC_045512	22716	.	C	T	19310.0	PASS	AF=0.967985;DP=531;DP4=0,0,297,234;SB=0;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aCt/aTt|T385I|1273|S|protein_coding|CODING|GU280_gp02|1|T)
+NC_045512	22775	.	G	A	49314.0	PASS	AF=0.881262;DP=2535;DP4=102,110,1368,953;SB=25;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gat/Aat|D405N|1273|S|protein_coding|CODING|GU280_gp02|1|A)
+NC_045512	22786	.	A	C	49314.0	PASS	AF=0.941202;DP=2347;DP4=0,1,1367,974;SB=3;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|agA/agC|R408S|1273|S|protein_coding|CODING|GU280_gp02|1|C)
+NC_045512	22792	.	C	T	49314.0	PASS	AF=0.966311;DP=2345;DP4=1,1,1366,976;SB=0;EFF=SYNONYMOUS_CODING(LOW|SILENT|atC/atT|I410|1273|S|protein_coding|CODING|GU280_gp02|1|T)
+NC_045512	22813	.	G	T	49314.0	PASS	AF=0.969281;DP=2474;DP4=0,1,1430,1042;SB=3;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aaG/aaT|K417N|1273|S|protein_coding|CODING|GU280_gp02|1|T)
+NC_045512	22882	.	T	G	49314.0	PASS	AF=0.958710;DP=1768;DP4=2,1,1209,555;SB=0;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aaT/aaG|N440K|1273|S|protein_coding|CODING|GU280_gp02|1|G)
+NC_045512	22992	.	G	A	36772.0	PASS	AF=0.964355;DP=1038;DP4=0,0,475,563;SB=0;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aGc/aAc|S477N|1273|S|protein_coding|CODING|GU280_gp02|1|A)
+NC_045512	22995	.	C	A	37275.0	PASS	AF=0.970760;DP=1026;DP4=0,0,471,555;SB=0;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aCa/aAa|T478K|1273|S|protein_coding|CODING|GU280_gp02|1|A)
+NC_045512	23013	.	A	C	36561.0	PASS	AF=0.965174;DP=1005;DP4=2,0,456,545;SB=6;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gAa/gCa|E484A|1273|S|protein_coding|CODING|GU280_gp02|1|C)
+NC_045512	23040	.	A	G	34886.0	PASS	AF=0.978079;DP=958;DP4=0,1,442,515;SB=0;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cAa/cGa|Q493R|1273|S|protein_coding|CODING|GU280_gp02|1|G)
+NC_045512	23055	.	A	G	33387.0	PASS	AF=0.962065;DP=949;DP4=0,1,448,500;SB=0;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cAa/cGa|Q498R|1273|S|protein_coding|CODING|GU280_gp02|1|G)
+NC_045512	23063	.	A	T	33872.0	PASS	AF=0.985792;DP=915;DP4=0,0,449,464;SB=0;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Aat/Tat|N501Y|1273|S|protein_coding|CODING|GU280_gp02|1|T)
+NC_045512	23075	.	T	C	31911.0	PASS	AF=0.961538;DP=910;DP4=0,0,458,452;SB=0;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Tac/Cac|Y505H|1273|S|protein_coding|CODING|GU280_gp02|1|C)
+NC_045512	23403	.	A	G	12029.0	PASS	AF=0.972973;DP=333;DP4=0,0,161,172;SB=0;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gAt/gGt|D614G|1273|S|protein_coding|CODING|GU280_gp02|1|G)
+NC_045512	23525	.	C	T	11185.0	PASS	AF=0.973941;DP=307;DP4=0,0,164,143;SB=0;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cat/Tat|H655Y|1273|S|protein_coding|CODING|GU280_gp02|1|T)
+NC_045512	23599	.	T	G	49314.0	PASS	AF=0.958807;DP=1408;DP4=0,1,739,667;SB=3;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aaT/aaG|N679K|1273|S|protein_coding|CODING|GU280_gp02|1|G)
+NC_045512	23604	.	C	A	49314.0	PASS	AF=0.968750;DP=1408;DP4=0,0,740,668;SB=0;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cCt/cAt|P681H|1273|S|protein_coding|CODING|GU280_gp02|1|A)
+NC_045512	23854	.	C	A	18583.0	PASS	AF=0.942379;DP=538;DP4=1,2,258,276;SB=0;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aaC/aaA|N764K|1273|S|protein_coding|CODING|GU280_gp02|1|A)
+NC_045512	23948	.	G	T	21728.0	PASS	AF=0.959933;DP=599;DP4=0,0,306,293;SB=0;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gat/Tat|D796Y|1273|S|protein_coding|CODING|GU280_gp02|1|T)
+NC_045512	24424	.	A	T	49314.0	PASS	AF=0.950000;DP=2320;DP4=1,1,1222,1094;SB=0;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|caA/caT|Q954H|1273|S|protein_coding|CODING|GU280_gp02|1|T)
+NC_045512	24469	.	T	A	49314.0	PASS	AF=0.951425;DP=2491;DP4=1,1,1302,1183;SB=0;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aaT/aaA|N969K|1273|S|protein_coding|CODING|GU280_gp02|1|A)
+NC_045512	24966	.	A	G	31324.0	PASS	AF=0.953933;DP=890;DP4=0,0,408,477;SB=0;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aAc/aGc|N1135S|1273|S|protein_coding|CODING|GU280_gp02|1|G)
+NC_045512	25000	.	C	T	30573.0	PASS	AF=0.961131;DP=849;DP4=1,0,382,463;SB=3;EFF=SYNONYMOUS_CODING(LOW|SILENT|gaC/gaT|D1146|1273|S|protein_coding|CODING|GU280_gp02|1|T)
+NC_045512	25584	.	C	T	41217.0	PASS	AF=0.963781;DP=1132;DP4=1,3,726,402;SB=8;EFF=SYNONYMOUS_CODING(LOW|SILENT|acC/acT|T64|275|ORF3a|protein_coding|CODING|GU280_gp03|1|T)
+NC_045512	26060	.	C	T	41883.0	PASS	AF=0.971706;DP=1131;DP4=0,0,506,622;SB=0;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aCt/aTt|T223I|275|ORF3a|protein_coding|CODING|GU280_gp03|1|T)
+NC_045512	26195	.	C	T	7270.0	PASS	AF=0.980099;DP=201;DP4=0,0,92,109;SB=0;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aCg/aTg|T268M|275|ORF3a|protein_coding|CODING|GU280_gp03|1|T)
+NC_045512	26270	.	C	T	6219.0	PASS	AF=0.970930;DP=172;DP4=0,0,95,77;SB=0;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aCa/aTa|T9I|75|E|protein_coding|CODING|GU280_gp04|1|T)
+NC_045512	26577	.	C	G	19291.0	PASS	AF=0.961039;DP=539;DP4=0,0,247,292;SB=0;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Caa/Gaa|Q19E|222|M|protein_coding|CODING|GU280_gp05|1|G)
+NC_045512	26709	.	G	A	49071.0	PASS	AF=0.968148;DP=1350;DP4=1,1,848,498;SB=0;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gct/Act|A63T|222|M|protein_coding|CODING|GU280_gp05|1|A)
+NC_045512	26801	.	C	T	49314.0	PASS	AF=0.961560;DP=1795;DP4=0,0,913,881;SB=0;EFF=SYNONYMOUS_CODING(LOW|SILENT|ctC/ctT|L93|222|M|protein_coding|CODING|GU280_gp05|1|T)
+NC_045512	26858	.	C	T	49314.0	PASS	AF=0.979244;DP=1349;DP4=0,0,509,839;SB=0;EFF=SYNONYMOUS_CODING(LOW|SILENT|ttC/ttT|F112|222|M|protein_coding|CODING|GU280_gp05|1|T)
+NC_045512	27259	.	A	C	49314.0	PASS	AF=0.973325;DP=1537;DP4=1,1,428,1105;SB=3;EFF=SYNONYMOUS_CODING(LOW|SILENT|Agg/Cgg|R20|61|ORF6|protein_coding|CODING|GU280_gp06|1|C)
+NC_045512	27382	.	G	C	7064.0	PASS	AF=0.954082;DP=196;DP4=0,0,95,101;SB=0;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gat/Cat|D61H|61|ORF6|protein_coding|CODING|GU280_gp06|1|C)
+NC_045512	27383	.	A	T	7052.0	PASS	AF=0.958549;DP=193;DP4=0,0,94,99;SB=0;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gAt/gTt|D61V|61|ORF6|protein_coding|CODING|GU280_gp06|1|T)
+NC_045512	27384	.	T	C	7096.0	PASS	AF=0.953846;DP=195;DP4=1,1,94,99;SB=0;EFF=SYNONYMOUS_CODING(LOW|SILENT|gaT/gaC|D61|61|ORF6|protein_coding|CODING|GU280_gp06|1|C)
+NC_045512	27481	.	C	T	10114.0	PASS	AF=0.971530;DP=281;DP4=0,0,151,130;SB=0;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ctt/Ttt|L30F|121|ORF7a|protein_coding|CODING|GU280_gp07|1|T)
+NC_045512	27549	.	C	T	38892.0	PASS	AF=0.978988;DP=1047;DP4=0,0,543,504;SB=0;EFF=SYNONYMOUS_CODING(LOW|SILENT|aaC/aaT|N52|121|ORF7a|protein_coding|CODING|GU280_gp07|1|T)
+NC_045512	27807	.	C	T	10876.0	PASS	AF=0.967427;DP=307;DP4=1,0,152,154;SB=3;EFF=SYNONYMOUS_CODING(LOW|SILENT|Cta/Tta|L18|43|ORF7b|protein_coding|CODING|GU280_gp08|1|T)
+NC_045512	28271	.	A	T	49314.0	PASS	AF=0.971286;DP=2403;DP4=1,1,1260,1141;SB=0
+NC_045512	28311	.	C	T	49314.0	PASS	AF=0.913405;DP=2402;DP4=2,4,1245,1144;SB=3;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cCc/cTc|P13L|419|N|protein_coding|CODING|GU280_gp10|1|T)
+NC_045512	28361	.	GGAGAACGCA	G	49314.0	PASS	AF=0.682846;DP=2122;DP4=617,58,445,1004;HRUN=2;SB=1661;INDEL;EFF=CODON_CHANGE_PLUS_CODON_DELETION(MODERATE||ggagaacgcagt/ggt|GERS30G|419|N|protein_coding|CODING|GU280_gp10|1|G|INFO_REALIGN_3_PRIME)
+NC_045512	28657	.	C	T	49314.0	PASS	AF=0.943368;DP=2013;DP4=1,0,996,1013;SB=3;EFF=SYNONYMOUS_CODING(LOW|SILENT|gaC/gaT|D128|419|N|protein_coding|CODING|GU280_gp10|1|T)
+NC_045512	28881	.	G	A	20426.0	PASS	AF=0.958929;DP=560;DP4=1,0,283,276;SB=0;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aGg/aAg|R203K|419|N|protein_coding|CODING|GU280_gp10|1|A)
+NC_045512	28882	.	G	A	19564.0	PASS	AF=0.928571;DP=560;DP4=0,0,284,274;SB=0;EFF=SYNONYMOUS_CODING(LOW|SILENT|agG/agA|R203|419|N|protein_coding|CODING|GU280_gp10|1|A)
+NC_045512	28883	.	G	C	20254.0	PASS	AF=0.952297;DP=566;DP4=0,0,285,280;SB=0;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gga/Cga|G204R|419|N|protein_coding|CODING|GU280_gp10|1|C)
+NC_045512	29510	.	A	C	49314.0	PASS	AF=0.958883;DP=1970;DP4=0,2,778,1187;SB=2;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Agt/Cgt|S413R|419|N|protein_coding|CODING|GU280_gp10|1|C)
+NC_045512	29733	.	CGAGGCCACGCGGAGTACGATCGAGTG	C	49314.0	PASS	AF=0.995888;DP=1216;DP4=89,86,224,987;HRUN=1;SB=180;INDEL