Mercurial > repos > iuc > vt_variant_tools
changeset 0:106dc0bf83c6 draft
Imported from capsule None
author | bgruening |
---|---|
date | Thu, 14 Aug 2014 07:41:51 -0400 |
parents | |
children | 16f7587ab084 |
files | readme.rst tool-data/fasta_indexes.loc.sample tool_data_table_conf.xml.sample tool_dependencies.xml vt_macros.xml vt_normalize.xml |
diffstat | 6 files changed, 213 insertions(+), 0 deletions(-) [+] |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/readme.rst Thu Aug 14 07:41:51 2014 -0400 @@ -0,0 +1,52 @@ +========================================== +Galaxy wrapper for VT the Variant Tool Set +========================================== + +A tool set for short variant discovery in genetic sequence data. + +http://genome.sph.umich.edu/wiki/vt + +============ +Installation +============ + +It is recommended to install this wrapper via the `Galaxy Tool Shed`. + +.. _`Galaxy Tool Shed`: https://testtoolshed.g2.bx.psu.edu/view/iuc/vt + + +======= +History +======= +- 0.1: Initial public release + + +==================== +Detailed description +==================== + +View the original GEMINI documentation: http://genome.sph.umich.edu/wiki/vt + + +=============================== +Wrapper Licence (MIT/BSD style) +=============================== + +Permission to use, copy, modify, and distribute this software and its +documentation with or without modifications and for any purpose and +without fee is hereby granted, provided that any copyright notices +appear in all copies and that both those copyright notices and this +permission notice appear in supporting documentation, and that the +names of the contributors or copyright holders not be used in +advertising or publicity pertaining to distribution of the software +without specific prior permission. + +THE CONTRIBUTORS AND COPYRIGHT HOLDERS OF THIS SOFTWARE DISCLAIM ALL +WARRANTIES WITH REGARD TO THIS SOFTWARE, INCLUDING ALL IMPLIED +WARRANTIES OF MERCHANTABILITY AND FITNESS, IN NO EVENT SHALL THE +CONTRIBUTORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY SPECIAL, INDIRECT +OR CONSEQUENTIAL DAMAGES OR ANY DAMAGES WHATSOEVER RESULTING FROM LOSS +OF USE, DATA OR PROFITS, WHETHER IN AN ACTION OF CONTRACT, NEGLIGENCE +OR OTHER TORTIOUS ACTION, ARISING OUT OF OR IN CONNECTION WITH THE USE +OR PERFORMANCE OF THIS SOFTWARE. +
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/tool-data/fasta_indexes.loc.sample Thu Aug 14 07:41:51 2014 -0400 @@ -0,0 +1,29 @@ +#This is a sample file distributed with Galaxy that enables tools +#to use a directory of Samtools indexed sequences data files. You will need +#to create these data files and then create a fasta_indexes.loc file +#similar to this one (store it in this directory) that points to +#the directories in which those files are stored. The fasta_indexes.loc +#file has this format (white space characters are TAB characters): +# +# <unique_build_id> <dbkey> <display_name> <file_base_path> +# +#So, for example, if you had hg19 Canonical indexed stored in +# +# /depot/data2/galaxy/hg19/sam/, +# +#then the fasta_indexes.loc entry would look like this: +# +#hg19canon hg19 Human (Homo sapiens): hg19 Canonical /depot/data2/galaxy/hg19/sam/hg19canon.fa +# +#and your /depot/data2/galaxy/hg19/sam/ directory +#would contain hg19canon.fa and hg19canon.fa.fai files. +# +#Your fasta_indexes.loc file should include an entry per line for +#each index set you have stored. The file in the path does actually +#exist, but it should never be directly used. Instead, the name serves +#as a prefix for the index file. For example: +# +#hg18canon hg18 Human (Homo sapiens): hg18 Canonical /depot/data2/galaxy/hg18/sam/hg18canon.fa +#hg18full hg18 Human (Homo sapiens): hg18 Full /depot/data2/galaxy/hg18/sam/hg18full.fa +#hg19canon hg19 Human (Homo sapiens): hg19 Canonical /depot/data2/galaxy/hg19/sam/hg19canon.fa +#hg19full hg19 Human (Homo sapiens): hg19 Full /depot/data2/galaxy/hg19/sam/hg19full.fa
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/tool_data_table_conf.xml.sample Thu Aug 14 07:41:51 2014 -0400 @@ -0,0 +1,7 @@ +<!-- Use the file tool_data_table_conf.xml.oldlocstyle if you don't want to update your loc files as changed in revision 4550:535d276c92bc--> +<tables> + <table name="fasta_indexes" comment_char="#"> + <columns>value, dbkey, name, path</columns> + <file path="tool-data/fasta_indexes.loc" /> + </table> +</tables>
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/tool_dependencies.xml Thu Aug 14 07:41:51 2014 -0400 @@ -0,0 +1,6 @@ +<?xml version="1.0"?> +<tool_dependency> + <package name="vt" version="5c735ab14b5603d9f14da6ee0e63d86ba3779934"> + <repository changeset_revision="91635ed3dceb" name="package_vt_5c735ab14b5603d9f14da6ee0e63d86ba3779934" owner="iuc" toolshed="https://toolshed.g2.bx.psu.edu" /> + </package> +</tool_dependency>
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/vt_macros.xml Thu Aug 14 07:41:51 2014 -0400 @@ -0,0 +1,42 @@ +<macros> + <xml name="requirements"> + <requirements> + <requirement type="package" version="5c735ab14b5603d9f14da6ee0e63d86ba3779934">vt</requirement> + <yield /> + </requirements> + </xml> + + <xml name="version_command"> + <version_command><![CDATA[ + vt view --help 2>&1 >/dev/null | head -n 1 + ]]> + </version_command> + </xml> + + <xml name="stdio"> + <stdio> + <exit_code range="1:" /> + <exit_code range=":-1" /> + <regex match="Error:" /> + <regex match="Exception:" /> + </stdio> + </xml> + + <token name="@VERSION@">0.1</token> + + <token name="@CITATION@">------ + +**Citation** + +If you use VT in your research, please cite the following project site: + +https://github.com/atks/vt + + </token> + <!--xml name="citations"> + <citations> + <citation type="doi">10.1371/journal.pcbi.1003153</citation> + <yield /> + </citations> + </xml--> +</macros>
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/vt_normalize.xml Thu Aug 14 07:41:51 2014 -0400 @@ -0,0 +1,77 @@ +<tool id="vt_@BINARY@" name="VT @BINARY@" version="@VERSION@.0"> + <description>normalizes variants in a VCF file</description> + <expand macro="requirements" /> + <expand macro="version_command" /> + <macros> + <import>vt_macros.xml</import> + <token name="@BINARY@">normalize</token> + </macros> + <command> +<![CDATA[ + vt @BINARY@ + -o "${ outfile }" + ##-q do not print options and summary [] + -w window size for local sorting of variants [10000] + + #if str( $intervals_file ) != 'None': + -I "${intervals_file}" + #end if + + ##-i intervals [] + + #if $reference_source.reference_source_selector != "history": + -r "${reference_source.reference_genome.fields.path}"' + #else: + -r "${reference_source.reference_genome}" + #end if + + "${ infile }" +]]> + </command> + <expand macro="stdio" /> + <inputs> + <param name="infile" type="data" format="vcf" label="VCF file to be normalised" /> + + + <conditional name="reference_source"> + <param name="reference_source_selector" type="select" label="Choose the source for the reference list"> + <option value="cached" selected="True">Locally cached</option> + <option value="history">History</option> + </param> + <when value="cached"> + <param name="reference_genome" type="select" label="Using reference genome"> + <options from_data_table="fasta_indexes"> + <filter type="data_meta" ref="infile" key="dbkey" column="1" /> + <validator type="no_options" message="No reference genome is available for the build associated with the selected input dataset" /> + </options> + </param> + </when> + <when value="history"> + <param name="reference_genome" type="data" format="fasta" label="Using reference file" /> + </when> + </conditional> + + <param name="intervals_file" type="data" format="bed" optional="True" label="File containing list of intervals" /> + <param name="window" type="integer" value="10000" size="10" label="Window size for local sorting of variants" + help="(-w)"> + <validator type="in_range" min="0"/> + </param> + + </inputs> + <outputs> + <data name="outfile" format="vcf" label="${tool.name} on ${on_string}" /> + </outputs> + <tests> + <test> + </test> + </tests> + <help> +**What it does** + +Normalize variants in a VCF file. Normalized variants may have their positions changed; in such cases, +the normalized variants are reordered and output in an ordered fashion. The local reordering takes place over a window of 10000 base pairs. + +@CITATION@ + </help> + <!--expand macro="citations"/--> +</tool>