Mercurial > repos > jackcurragh > ribogalaxy_bowtie_rrna
comparison bowtie_rRNA_removal_wrapper/bowtie_rRNA_tRNA_removal_wrapper.xml @ 21:b49ad9f12dfa draft
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author | jackcurragh |
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date | Wed, 11 May 2022 13:37:35 +0000 |
parents | dbb3e98144fd |
children | ca7c965f7cc9 |
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20:87b1fa00d6ab | 21:b49ad9f12dfa |
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1 <tool id="bowtie_rRNA_tRNA_removal_wrapper" name="Remove rRNA and tRNA using Bowtie" version="1.4.0"> | 1 <tool id="bowtie_rRNA_tRNA_removal_wrapper" name="Remove rRNA and tRNA using Bowtie" version="1.5.0"> |
2 <description></description> | 2 <description></description> |
3 <requirements> | 3 <requirements> |
4 <requirement type="package" version="1.2.0">bowtie</requirement> | 4 <requirement type="package" version="1.2.0">bowtie</requirement> |
5 </requirements> | 5 </requirements> |
6 <version_command>bowtie --version</version_command> | 6 <version_command>bowtie --version</version_command> |
154 </command> | 154 </command> |
155 <inputs> | 155 <inputs> |
156 <conditional name="refGenomeSource"> | 156 <conditional name="refGenomeSource"> |
157 <param name="genomeSource" type="select" label="Will you select a reference genome from your history or use a built-in index?" help="Built-ins were indexed using default options"> | 157 <param name="genomeSource" type="select" label="Will you select a reference genome from your history or use a built-in index?" help="Built-ins were indexed using default options"> |
158 <option value="indexed">Use a built-in index</option> | 158 <option value="indexed">Use a built-in index</option> |
159 <option value="history" selected="True">Use one from the history</option> | 159 <option value="history">Use one from the history</option> |
160 </param> | 160 </param> |
161 <when value="indexed"> | 161 <when value="indexed"> |
162 <param name="index" type="select" label="Select a reference genome" help="if your genome of interest is not listed - contact Galaxy team"> | 162 <param name="index" type="select" label="Select a reference index" help="if your index of interest is not listed - contact Galaxy team"> |
163 <options from_data_table="bowtie_indexes"> | 163 <options from_data_table="bowtie_rRNA_indexes"> |
164 <filter type="sort_by" column="2" /> | 164 <filter type="sort_by" column="2" /> |
165 <validator type="no_options" message="No indexes are available" /> | 165 <validator type="no_options" message="No indexes are available" /> |
166 </options> | 166 </options> |
167 </param> | 167 </param> |
168 </when> | 168 </when> |
254 <param name="sForwardAlign" type="select" label="Choose whether or not to attempt to align against the forward reference strand (--nofw)"> | 254 <param name="sForwardAlign" type="select" label="Choose whether or not to attempt to align against the forward reference strand (--nofw)"> |
255 <option value="forward">Align against the forward reference strand</option> | 255 <option value="forward">Align against the forward reference strand</option> |
256 <option value="noForward">Do not align against the forward reference strand</option> | 256 <option value="noForward">Do not align against the forward reference strand</option> |
257 </param> | 257 </param> |
258 <param name="sReverseAlign" type="select" label="Choose whether or not to attempt to align against the reverse-complement reference strand (--norc)"> | 258 <param name="sReverseAlign" type="select" label="Choose whether or not to attempt to align against the reverse-complement reference strand (--norc)"> |
259 <option value="reverse">Align against the reverse-complement reference strand</option> | 259 <option value="reverse" selected="true">Align against the reverse-complement reference strand</option> |
260 <option value="noReverse" selected="true">Do not align against the reverse-complement reference strand</option> | 260 <option value="noReverse">Do not align against the reverse-complement reference strand</option> |
261 </param> | 261 </param> |
262 <conditional name="sBestOption"> | 262 <conditional name="sBestOption"> |
263 <param name="sBest" type="select" label="Whether or not to make Bowtie guarantee that reported singleton alignments are 'best' in terms of stratum and in terms of the quality values at the mismatched positions (--best)" help="Removes all strand bias. Only affects which alignments are reported by Bowtie. Runs slower with best option"> | 263 <param name="sBest" type="select" label="Whether or not to make Bowtie guarantee that reported singleton alignments are 'best' in terms of stratum and in terms of the quality values at the mismatched positions (--best)" help="Removes all strand bias. Only affects which alignments are reported by Bowtie. Runs slower with best option"> |
264 <option value="noBest">Do not use best</option> | 264 <option value="noBest">Do not use best</option> |
265 <option value="doBest">Use best</option> | 265 <option value="doBest">Use best</option> |
350 <option value="round">Round to nearest 10</option> | 350 <option value="round">Round to nearest 10</option> |
351 <option value="noRound">Do not round to nearest 10</option> | 351 <option value="noRound">Do not round to nearest 10</option> |
352 </param> | 352 </param> |
353 </when> | 353 </when> |
354 <when value="vMode"> | 354 <when value="vMode"> |
355 <param name="maxMismatches" type="integer" value="" min="0" max="3" label="Maximum number of mismatches (-v)" help="May be 0, 1, 2, or 3" /> | 355 <param name="maxMismatches" type="integer" value="3" min="0" max="3" label="Maximum number of mismatches (-v)" help="May be 0, 1, 2, or 3" /> |
356 <param name="pSeedLen" type="integer" value="25" min="5" label="Seed length (-l)" help="Minimum value is 5" /> | 356 <param name="pSeedLen" type="integer" value="25" min="5" label="Seed length (-l)" help="Minimum value is 5" /> |
357 </when> | 357 </when> |
358 </conditional> | 358 </conditional> |
359 <param name="pMinInsert" type="integer" value="0" label="Minimum insert size for valid paired-end alignments (-I)" /> | 359 <param name="pMinInsert" type="integer" value="0" label="Minimum insert size for valid paired-end alignments (-I)" /> |
360 <param name="pForwardAlign" type="select" label="Choose whether or not to attempt to align against the forward reference strand (--nofw)"> | 360 <param name="pForwardAlign" type="select" label="Choose whether or not to attempt to align against the forward reference strand (--nofw)"> |
361 <option value="forward">Align against the forward reference strand</option> | 361 <option value="forward">Align against the forward reference strand</option> |
362 <option value="noForward">Do not align against the forward reference strand</option> | 362 <option value="noForward">Do not align against the forward reference strand</option> |
363 </param> | 363 </param> |
364 <param name="pReverseAlign" type="select" label="Choose whether or not to attempt to align against the reverse-complement reference strand (--norc)"> | 364 <param name="pReverseAlign" type="select" label="Choose whether or not to attempt to align against the reverse-complement reference strand (--norc)"> |
365 <option value="reverse">Align against the reverse-complement reference strand</option> | 365 <option value="reverse" selected='true'>Align against the reverse-complement reference strand</option> |
366 <option value="noReverse" selected="true">Do not align against the reverse-complement reference strand</option> | 366 <option value="noReverse">Do not align against the reverse-complement reference strand</option> |
367 </param> | 367 </param> |
368 <conditional name="pBestOption"> | 368 <conditional name="pBestOption"> |
369 <param name="pBest" type="select" label="Whether or not to make Bowtie guarantee that reported singleton alignments are 'best' in terms of stratum and in terms of the quality values at the mismatched positions (--best)" help="Removes all strand bias. Only affects which alignments are reported by Bowtie. Runs slower with best option"> | 369 <param name="pBest" type="select" label="Whether or not to make Bowtie guarantee that reported singleton alignments are 'best' in terms of stratum and in terms of the quality values at the mismatched positions (--best)" help="Removes all strand bias. Only affects which alignments are reported by Bowtie. Runs slower with best option"> |
370 <option value="noBest">Do not use best</option> | 370 <option value="noBest">Do not use best</option> |
371 <option value="doBest">Use best</option> | 371 <option value="doBest">Use best</option> |
418 <param name="pSeed" type="integer" value="-1" label="Seed for pseudo-random number generator (--seed)" help="-1 for default" /> | 418 <param name="pSeed" type="integer" value="-1" label="Seed for pseudo-random number generator (--seed)" help="-1 for default" /> |
419 </when> <!-- full --> | 419 </when> <!-- full --> |
420 </conditional> <!-- pParams --> | 420 </conditional> <!-- pParams --> |
421 </when> <!-- paired --> | 421 </when> <!-- paired --> |
422 </conditional> <!-- singlePaired --> | 422 </conditional> <!-- singlePaired --> |
423 <param name="save_mapped_reads" type="boolean" checked="False" label="Same the bowtie alignments to the non-coding RNA" /> | |
423 <param name="save_mapping_stats" type="boolean" checked="True" label="Save the bowtie mapping statistics to the history" /> | 424 <param name="save_mapping_stats" type="boolean" checked="True" label="Save the bowtie mapping statistics to the history" /> |
424 <param name="suppressHeader" type="boolean" truevalue="true" falsevalue="false" checked="False" label="Suppress the header in the output SAM file (--sam-nohead)" help="Bowtie produces SAM with several lines of header information by default" /> | 425 <param name="suppressHeader" type="boolean" truevalue="true" falsevalue="false" checked="False" label="Suppress the header in the output SAM file (--sam-nohead)" help="Bowtie produces SAM with several lines of header information by default" /> |
425 </inputs> | 426 </inputs> |
426 <outputs> | 427 <outputs> |
427 <data format="sam" name="output" label="${tool.name} on ${on_string}: mapped reads"> | 428 <data format="sam" name="output" label="${tool.name} on ${on_string}: mapped reads"> |
429 <filter>save_mapped_reads is True</filter> | |
428 <actions> | 430 <actions> |
429 <conditional name="refGenomeSource.genomeSource"> | 431 <conditional name="refGenomeSource.genomeSource"> |
430 <when value="indexed"> | 432 <when value="indexed"> |
431 <action type="metadata" name="dbkey"> | 433 <action type="metadata" name="dbkey"> |
432 <option type="from_data_table" name="bowtie_indexes" column="1" offset="0"> | 434 <option type="from_data_table" name="bowtie_rRNA_indexes" column="1" offset="0"> |
433 <filter type="param_value" column="0" value="#" compare="startswith" keep="False"/> | 435 <filter type="param_value" column="0" value="#" compare="startswith" keep="False"/> |
434 <filter type="param_value" ref="refGenomeSource.index" column="0"/> | 436 <filter type="param_value" ref="refGenomeSource.index" column="0"/> |
435 </option> | 437 </option> |
436 </action> | 438 </action> |
437 </when> | 439 </when> |
538 </actions> | 540 </actions> |
539 </data> | 541 </data> |
540 </outputs> | 542 </outputs> |
541 <tests> | 543 <tests> |
542 <test> | 544 <test> |
543 <!-- | 545 <param name="genomeSource" value="history" /> |
544 Bowtie command: | 546 <param name="ownFile" value="chr9_100000bases.fa" /> |
545 bowtie -q -p 4 -S +sam-nohead chrM_base test-data/bowtie_in2.fastqsanger > bowtie_out6_u.sam | 547 <param name="indexSettings" value="indexPreSet" /> |
546 sort bowtie_out6_u.sam > bowtie_out6.sam | |
547 -p is the number of threads. You need to replace the + with 2 dashes. | |
548 chrM_base needs to be the base location/name of the index files. | |
549 --> | |
550 <param name="genomeSource" value="indexed" /> | |
551 <!-- this is the backwards-compatible "unique value" for this index, not an actual path --> | |
552 <param name="index" value="equCab2chrM" /> | |
553 <param name="sPaired" value="single" /> | 548 <param name="sPaired" value="single" /> |
554 <param name="sInput1" ftype="fastqsanger" value="bowtie_in2.fastqsanger" /> | 549 <param name="sInput1" ftype="fastqsanger" value="test.fastq" /> |
555 <param name="sSettingsType" value="preSet" /> | 550 <param name="alignMode" value="vMode" /> |
556 <param name="suppressHeader" value="true" /> | 551 <output name="output_unmapped_reads_l" ftype="fastqsanger" file="lesschr9.fastq" /> |
557 <output name="output" ftype="sam" file="bowtie_out6.sam" sort="True"> | |
558 <metadata name="dbkey" value="equCab2" /> | |
559 </output> | |
560 </test> | 552 </test> |
561 <test> | 553 |
562 <!-- | |
563 Bowtie command: | |
564 bowtie-build -f test-data/phiX.fasta phiX_base | |
565 bowtie -q -X 1000 +ff -p 4 -S +sam-nohead -n 2 -e 70 -l 28 +pairtries 100 +maxbts 800 +best +un bowtie_out8_u.fastq phiX_base -1 test-data/bowtie_in5.fastqsanger -2 test-data/bowtie_in6.fastqsanger > bowtie_out7_u.sam | |
566 sort bowtie_out7_u.sam > bowtie_out7.sam | |
567 sort bowtie_out8_u_1.sam > bowtie_out8_1.sam | |
568 sort bowtie_out8_u_2.sam > bowtie_out8_2.sam | |
569 Then also need to modify bowtie_out8_1.sam and bowtie_out8_2.sam so that all @ lines come before sequence lines. | |
570 -p is the number of threads. You need to replace the + with 2 dashes. | |
571 The two unmapped output files will be named bowtie_out8_1.fastq and bowtie_out8_2.fastq. | |
572 chrM_base is the index files' location/base name. | |
573 --> | |
574 <param name="genomeSource" value="history" /> | |
575 <param name="ownFile" value="phiX.fasta" /> | |
576 <param name="indexSettings" value="indexPreSet" /> | |
577 <param name="sPaired" value="paired" /> | |
578 <param name="pInput1" ftype="fastqsanger" value="bowtie_in5.fastqsanger" /> | |
579 <param name="pInput2" ftype="fastqsanger" value="bowtie_in6.fastqsanger" /> | |
580 <param name="pMaxInsert" value="1000" /> | |
581 <param name="pMateOrient" value="ff" /> | |
582 <param name="pSettingsType" value="full" /> | |
583 <param name="pSkip" value="0" /> | |
584 <param name="pAlignLimit" value="-1" /> | |
585 <param name="pTrimH" value="0" /> | |
586 <param name="pTrimL" value="0" /> | |
587 <param name="alignMode" value="nMode" /> | |
588 <param name="pMismatchSeed" value="2" /> | |
589 <param name="pMismatchQual" value="70" /> | |
590 <param name="pSeedLen" value="28" /> | |
591 <param name="pRounding" value="round" /> | |
592 <param name="pMinInsert" value="0" /> | |
593 <param name="pMaxAlignAttempt" value="100" /> | |
594 <param name="pForwardAlign" value="forward" /> | |
595 <param name="pReverseAlign" value="reverse" /> | |
596 <param name="pTryHard" value="noTryHard" /> | |
597 <param name="pValAlign" value="1" /> | |
598 <param name="pAllValAligns" value="noAllValAligns" /> | |
599 <param name="pSuppressAlign" value="-1" /> | |
600 <param name="pUnmappedFile" value="true" /> | |
601 <param name="pMaxFile" value="false" /> | |
602 <param name="pBest" value="doBest" /> | |
603 <param name="pdMaxBacktracks" value="800" /> | |
604 <param name="pdStrata" value="noStrata" /> | |
605 <param name="pOffrate" value="-1" /> | |
606 <param name="pSeed" value="-1" /> | |
607 <param name="suppressHeader" value="true" /> | |
608 <output name="output" ftype="sam" file="bowtie_out7.sam" sort="True" /> | |
609 <output name="output_unmapped_reads_l" ftype="fastqsanger" file="bowtie_out8_1.fastq" sort="True" /> | |
610 <output name="output_unmapped_reads_r" ftype="fastqsanger" file="bowtie_out8_2.fastq" sort="True" /> | |
611 </test> | |
612 <!-- start testing of non-sanger variant fastq reads --> | |
613 <test> | |
614 <param name="genomeSource" value="history" /> | |
615 <param name="ownFile" value="phiX.fasta" /> | |
616 <param name="indexSettings" value="indexPreSet" /> | |
617 <param name="sPaired" value="paired" /> | |
618 <param name="pInput1" ftype="fastqillumina" value="bowtie_in5.fastqillumina" /> | |
619 <param name="pInput2" ftype="fastqillumina" value="bowtie_in6.fastqillumina" /> | |
620 <param name="pMaxInsert" value="1000" /> | |
621 <param name="pMateOrient" value="ff" /> | |
622 <param name="pSettingsType" value="full" /> | |
623 <param name="pSkip" value="0" /> | |
624 <param name="pAlignLimit" value="-1" /> | |
625 <param name="pTrimH" value="0" /> | |
626 <param name="pTrimL" value="0" /> | |
627 <param name="alignMode" value="nMode" /> | |
628 <param name="pMismatchSeed" value="2" /> | |
629 <param name="pMismatchQual" value="70" /> | |
630 <param name="pSeedLen" value="28" /> | |
631 <param name="pRounding" value="round" /> | |
632 <param name="pMinInsert" value="0" /> | |
633 <param name="pMaxAlignAttempt" value="100" /> | |
634 <param name="pForwardAlign" value="forward" /> | |
635 <param name="pReverseAlign" value="reverse" /> | |
636 <param name="pTryHard" value="noTryHard" /> | |
637 <param name="pValAlign" value="1" /> | |
638 <param name="pAllValAligns" value="noAllValAligns" /> | |
639 <param name="pSuppressAlign" value="-1" /> | |
640 <param name="pUnmappedFile" value="true" /> | |
641 <param name="pMaxFile" value="false" /> | |
642 <param name="pBest" value="doBest" /> | |
643 <param name="pdMaxBacktracks" value="800" /> | |
644 <param name="pdStrata" value="noStrata" /> | |
645 <param name="pOffrate" value="-1" /> | |
646 <param name="pSeed" value="-1" /> | |
647 <param name="suppressHeader" value="true" /> | |
648 <output name="output" ftype="sam" file="bowtie_out7.sam" sort="True" /> | |
649 <output name="output_unmapped_reads_l" ftype="fastqillumina" file="bowtie_out8_1.fastqillumina.sorted" sort="True" /> | |
650 <output name="output_unmapped_reads_r" ftype="fastqillumina" file="bowtie_out8_2.fastqillumina.sorted" sort="True" /> | |
651 </test> | |
652 <test> | |
653 <param name="genomeSource" value="history" /> | |
654 <param name="ownFile" value="phiX.fasta" /> | |
655 <param name="indexSettings" value="indexPreSet" /> | |
656 <param name="sPaired" value="paired" /> | |
657 <param name="pInput1" ftype="fastqsolexa" value="bowtie_in5.fastqsolexa" /> | |
658 <param name="pInput2" ftype="fastqsolexa" value="bowtie_in6.fastqsolexa" /> | |
659 <param name="pMaxInsert" value="1000" /> | |
660 <param name="pMateOrient" value="ff" /> | |
661 <param name="pSettingsType" value="full" /> | |
662 <param name="pSkip" value="0" /> | |
663 <param name="pAlignLimit" value="-1" /> | |
664 <param name="pTrimH" value="0" /> | |
665 <param name="pTrimL" value="0" /> | |
666 <param name="alignMode" value="nMode" /> | |
667 <param name="pMismatchSeed" value="2" /> | |
668 <param name="pMismatchQual" value="70" /> | |
669 <param name="pSeedLen" value="28" /> | |
670 <param name="pRounding" value="round" /> | |
671 <param name="pMinInsert" value="0" /> | |
672 <param name="pMaxAlignAttempt" value="100" /> | |
673 <param name="pForwardAlign" value="forward" /> | |
674 <param name="pReverseAlign" value="reverse" /> | |
675 <param name="pTryHard" value="noTryHard" /> | |
676 <param name="pValAlign" value="1" /> | |
677 <param name="pAllValAligns" value="noAllValAligns" /> | |
678 <param name="pSuppressAlign" value="-1" /> | |
679 <param name="pUnmappedFile" value="true" /> | |
680 <param name="pMaxFile" value="false" /> | |
681 <param name="pBest" value="doBest" /> | |
682 <param name="pdMaxBacktracks" value="800" /> | |
683 <param name="pdStrata" value="noStrata" /> | |
684 <param name="pOffrate" value="-1" /> | |
685 <param name="pSeed" value="-1" /> | |
686 <param name="suppressHeader" value="true" /> | |
687 <output name="output" ftype="sam" file="bowtie_out7.sam" sort="True" /> | |
688 <output name="output_unmapped_reads_l" ftype="fastqsolexa" file="bowtie_out8_1.fastqsolexa.sorted" sort="True" /> | |
689 <output name="output_unmapped_reads_r" ftype="fastqsolexa" file="bowtie_out8_2.fastqsolexa.sorted" sort="True" /> | |
690 </test> | |
691 <!-- end testing of non-sanger variant fastq reads --> | |
692 <test> | |
693 <!-- | |
694 Bowtie command: | |
695 bowtie -q -p 4 -S +sam-nohead -n 2 -e 70 -l 28 -y -k 1 chrM_base test-data/bowtie_in2.fastqsanger > bowtie_out9_u.sam | |
696 sort bowtie_out9_u.sam > bowtie_out9.sam | |
697 -p is the number of threads. You need to replace the + with 2 dashes. | |
698 chrM_base is the index files' location/base name. | |
699 --> | |
700 <param name="genomeSource" value="indexed" /> | |
701 <!-- this is the backwards-compatible "unique value" for this index, not an actual path --> | |
702 <param name="index" value="equCab2chrM" /> | |
703 <param name="sPaired" value="single" /> | |
704 <param name="sInput1" ftype="fastqsanger" value="bowtie_in2.fastqsanger" /> | |
705 <param name="sSettingsType" value="full" /> | |
706 <param name="sSkip" value="0" /> | |
707 <param name="sAlignLimit" value="-1" /> | |
708 <param name="sTrimH" value="0" /> | |
709 <param name="sTrimL" value="0" /> | |
710 <param name="alignMode" value="nMode" /> | |
711 <param name="sMismatchSeed" value="2" /> | |
712 <param name="sMismatchQual" value="70" /> | |
713 <param name="sSeedLen" value="28" /> | |
714 <param name="sRounding" value="round" /> | |
715 <param name="sForwardAlign" value="forward" /> | |
716 <param name="sReverseAlign" value="reverse" /> | |
717 <param name="sTryHard" value="doTryHard" /> | |
718 <param name="sValAlign" value="1" /> | |
719 <param name="sAllValAligns" value="noAllValAligns" /> | |
720 <param name="sSuppressAlign" value="-1" /> | |
721 <param name="sUnmappedFile" value="false" /> | |
722 <param name="sMaxFile" value="false" /> | |
723 <param name="sBest" value="noBest" /> | |
724 <param name="sOffrate" value="-1" /> | |
725 <param name="sSeed" value="-1" /> | |
726 <param name="suppressHeader" value="true" /> | |
727 <output name="output" ftype="sam" file="bowtie_out9.sam" sort="True"> | |
728 <metadata name="dbkey" value="equCab2" /> | |
729 </output> | |
730 </test> | |
731 <test> | |
732 <!-- | |
733 Bowtie command: | |
734 bowtie-build +offrate 5 +ftabchars 10 +little -f test-data/phiX.fasta phiX_base | |
735 bowtie -q -X 1000 +ff -p 4 -S +sam-nohead phiX_base -1 test-data/bowtie_in5.fastqsanger -2 test-data/bowtie_in6.fastqsanger > bowtie_out10_u.sam | |
736 sort bowtie_out10_u.sam > bowtie_out10.sam | |
737 -p is the number of threads. You need to replace the + with 2 dashes. | |
738 chrM_base is the index files' location/base name. | |
739 --> | |
740 <param name="genomeSource" value="history" /> | |
741 <param name="ownFile" value="phiX.fasta" /> | |
742 <param name="indexSettings" value="indexFull" /> | |
743 <param name="autoB" value="auto" /> | |
744 <param name="nodc" value="dc" /> | |
745 <param name="noref" value="ref" /> | |
746 <param name="offrate" value="5" /> | |
747 <param name="ftab" value="10" /> | |
748 <param name="ntoa" value="no" /> | |
749 <param name="endian" value="little" /> | |
750 <param name="seed" value="-1" /> | |
751 <param name="sPaired" value="paired" /> | |
752 <param name="pInput1" ftype="fastqsanger" value="bowtie_in5.fastqsanger" /> | |
753 <param name="pInput2" ftype="fastqsanger" value="bowtie_in6.fastqsanger" /> | |
754 <param name="pMaxInsert" value="1000" /> | |
755 <param name="pMateOrient" value="ff" /> | |
756 <param name="pSettingsType" value="preSet" /> | |
757 <param name="suppressHeader" value="true" /> | |
758 <output name="output" ftype="sam" file="bowtie_out10.sam" sort="True" /> | |
759 </test> | |
760 <test> | |
761 <!-- | |
762 Bowtie command: | |
763 bowtie-build +offrate 5 +ftabchars 10 +little -f test-data/phiX.fasta phiX_base | |
764 bowtie -q -X 1000 +ff -p 4 -S +sam-nohead phiX_base -1 test-data/bowtie_in5.fastqsanger -2 test-data/bowtie_in6.fastqsanger > bowtie_out10_u.sam | |
765 sort bowtie_out10_u.sam > bowtie_out10.sam | |
766 -p is the number of threads. You need to replace the + with 2 dashes. | |
767 chrM_base is the index files' location/base name. | |
768 --> | |
769 <param name="genomeSource" value="history" /> | |
770 <param name="ownFile" value="phiX.fasta" /> | |
771 <param name="indexSettings" value="indexFull" /> | |
772 <param name="autoB" value="auto" /> | |
773 <param name="nodc" value="dc" /> | |
774 <param name="noref" value="ref" /> | |
775 <param name="offrate" value="5" /> | |
776 <param name="ftab" value="10" /> | |
777 <param name="ntoa" value="no" /> | |
778 <param name="endian" value="little" /> | |
779 <param name="seed" value="-1" /> | |
780 <param name="sPaired" value="paired" /> | |
781 <param name="pInput1" ftype="fastqsanger" value="bowtie_in5.fastqsanger" /> | |
782 <param name="pInput2" ftype="fastqsanger" value="bowtie_in6.fastqsanger" /> | |
783 <param name="pMaxInsert" value="1000" /> | |
784 <param name="pMateOrient" value="ff" /> | |
785 <param name="pSettingsType" value="preSet" /> | |
786 <param name="suppressHeader" value="true" /> | |
787 <param name="save_mapping_stats" value="true" /> | |
788 <output name="output" ftype="sam" file="bowtie_out10.sam" sort="True" /> | |
789 <output name="mapping_stats" ftype="txt" file="bowtie_out11.txt" sort="True" /> | |
790 </test> | |
791 </tests> | 554 </tests> |
792 | 555 |
793 <help> | 556 <help> |
794 | 557 |
795 **What it does** | 558 **What it does** |