view PDAUG_Fishers_Plot/PDAUG_Fishers_Plot.xml @ 4:3bde5f0d28d9 draft

"planemo upload for repository https://github.com/jaidevjoshi83/pdaug commit edb37634e419f75dd66292e712de51278746d883"

<tool id="pdaug_fishers_plot" name="PDAUG Fisher's Plot" version="0.1.0" >
	<description>Analyze peptide sequence data based on feature spaces </description>
    <requirements>
      <requirement type="package" version="2.7.18">python</requirement>
      <requirement type="package" version="1.4.6">powerlaw</requirement>
      <requirement type="package" version="0.2.4">quantiprot</requirement>
    </requirements>

    <command detect_errors="exit_code"><![CDATA[

        python '$__tool_directory__/PDAUG_Fishers_Plot.py'  --Fasta1 '$Fasta1' --Fasta2 '$Fasta2' --overlap_factor '$overlap_factor' --windows_per_frame '$windows_per_frame' --xlabel '$xlabel' --ylabel '$ylabel' --pop1_label '$pop1_label' --pop2_label '$pop2_label' --htmlOutDir '$output2.extra_files_path'  --htmlFname '$output2'
             
    ]]></command>

    <inputs>
      <param name="Fasta1" type="data"  format="fasta" label="First fasta file" argument="--Fasta1" help="Fasta file with peptides" />
      <param name="Fasta2" type="data"  format="fasta" label="Second fasta file" argument="--Fasta2" help="Fasta file with peptides" />
      <param name="overlap_factor" type="integer" value="5" label="Overlap factor" argument="--overlap_factor" help="Overlapping factor" />
      <param name="windows_per_frame" type="integer" value="5" label="Windows per frame" argument="--windows_per_frame" help="" />
      <param name="xlabel" type="text"  label="X-label" value="Mean volume" argument="--xlabel" help="Description of x label" />
      <param name="ylabel" type="text"  label="Y-label" value="Mean hydropathy" argument="--ylabel" help="Description of y label" />
      <param name="pop1_label" type="text" label="Label for first population" value="amyloids" argument="--pop1_label" help="Label for first set of peptide sequences" />
      <param name="pop2_label" type="text" label="Label for second population" value="non amyloids" argument="--pop2_label" help="Label for second set of peptide sequences" />
    </inputs>

    <outputs>   
        <data name='output2' format='png' label="${tool.name} on $on_string - (PNG)" from_work_dir="out.png"/>
    </outputs>
 
    <tests>
        <test>
            <param name="Fasta1" value="positive.fasta"/>
            <param name="Fasta2" value="negative.fasta"/>
            <param name="overlap_factor" value="5"  />
            <param name="windows_per_frame" value="5"  />
            <param name="xlabel" value="mean volume" />
            <param name="ylabel" value="mean hydropathy" />
            <param name="pop1_label"  value="Anticancer" />
            <param name="pop2_label"  value="anti-cancer" />
            <output name="output2" value="out.png" />
        </test>
    </tests>



    <help><![CDATA[
.. class:: infomark

**What it does**

This tool compares two peptide sequence data sets in a 2D space represented by quantitative features of peptides. The tool calculates a local ratio of the number of sequences from each peptide set in the part of the feature space and compares it to the global ratio in the entire feature space by Fisher’s exact test.

-----

**Inputs**
  * **--Fasta1** The First fasta file. 
  * **--Fasta2** The Second fasta file.
  * **--overlap_factor** Overlapping factor.
  * **--windows_per_frame** Windows per frame.
  * **--xlabel** X label for the plot.
  * **--ylabel** Y label for the plot.
  * **--pop1_label** Label for the first set of sequences. 
  * **--pop2_label** Label for the second set of sequences. 

-----

**Outputs**
    * Returrns html report file.]]></help>
<citations>
  <citation type="bibtex">
    @misc{PDAUGGITHUB, 
      author = {Joshi, Jayadev  and Blankenberg, Daniel}, 
      year = {2020}, 
      title ={PDAUG - a Galaxy based toolset for peptide library analysis, visualization, and machine learning modeling}, 
      publisher = {GitHub}, 
      journal = {GitHub repository}, 
      url =
      {https://github.com/jaidevjoshi83/pdaug.git}, }
  </citation>
  <citation type="bibtex">
    @article{konopka_marciniak_dyrka_2017,
       title={Quantiprot - a Python package for quantitative analysis of protein sequences}, 
       volume={18},
       DOI={10.1186/s12859-017-1751-4}, 
       number={1}, 
       journal={BMC Bioinformatics},
       author={Konopka, Bogumił M. and Marciniak, Marta and Dyrka, Witold}, 
       year={2017},
     }
  </citation>
</citations>
</tool>