view cpo_snippy.xml @ 5:698579246d0d draft

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<tool id="cpo_snippy" name="CPO_Snippy" version="3.2">
  <description>Modified Snippy v3.2 to support contig inputs for the cpo_prediction workflow</description>
  <requirements>
    <requirement type="package" version="3.2">snippy</requirement>
  </requirements>
  <stdio>
    <exit_code range="1:" />
  </stdio>

  <command>
    <![CDATA[
      snippy
      --outdir out
      --cpus "\${GALAXY_SLOTS:-1}"
      --ref $ref
      $cleanup
      #if str( $advanced.is_advanced ) == "advanced"
        --mapqual $advanced.mapqual
        --mincov $advanced.mincov
        --minfrac $advanced.minfrac
        #if $advanced.rgid
          --rgid $advanced.rgid
        #end if
        #if $advanced.bwaopt
          --bwaopt $advanced.bwaopt
        #end if
      #end if
      --ctgs $input

      &&

      gunzip out/snps.depth.gz

      &&

      #import re
      #set $dir_name = re.sub('[^\w_]', '_', $input.element_identifier)
      mkdir -p ${dir_name}/reference && cp out/snps.tab out/snps.aligned.fa ${dir_name}/ && cp out/reference/ref.fa ${dir_name}/reference/ &&

      tar -czf out.tgz ${dir_name}


    ]]>
  </command>
  <inputs>

    <param name="ref" type="data" format="fasta" label="Reference Fasta" />
    <param name="input" type="data" format="fasta" label="assembled contigs"/>
    <param name="cleanup" type="boolean" checked="true" truevalue="--cleanup" falsevalue="" label="Cleanup the non-snp output files" help="Remove all non-SNP files: BAMs, indices etc" />
    <conditional name="advanced">
      <param name="is_advanced" type="select" label="Advanced parameters" help="unhide advanced parameter settings">
        <option value="advanced">Show advanced settings</option>
        <option value="simple" selected="true">Hide advanced settings</option>
      </param>
      <when value="advanced">
        <param name="mapqual" type="float" value="60" label="Minimum mapping quality" help="Minimum mapping quality to allow" />
        <param name="mincov" type="float" value="10" label="Minimum coverage" help="Minimum coverage to call a snp" />
        <param name="minfrac" type="float" value="0.9" label="Minumum proportion for variant evidence" help="Minumum proportion for variant evidence" />
        <param name="rgid" type="text" value="" label="Bam header @RG ID" help="Use this @RG ID: in the BAM header" />
        <param name="bwaopt" type="text" value="" label="Extra BWA MEM options" help="Extra BWA MEM options, eg. -x pacbio" />
      </when>
      <when value="simple">

      </when>
    </conditional>
  </inputs>
  <outputs>
    <data format="vcf" name="snpvcf" label="${tool.name} on ${on_string} snps vcf file" from_work_dir="out/snps.vcf"/>
    <data format="gff3" name="snpgff" label="${tool.name} on ${on_string} snps gff file" from_work_dir="out/snps.gff"/>
    <data format="tabular" name="snptab" label="${tool.name} on ${on_string} snps table" from_work_dir="out/snps.tab"/>
    <data format="tabular" name="snpsum" label="${tool.name} on ${on_string} snps summary" from_work_dir="out/snps.txt"/>
    <data format="txt" name="snplog" label="${tool.name} on ${on_string} log file" from_work_dir="out/snps.log"/>
    <data format="fasta" name="snpalign" label="${tool.name} on ${on_string} aligned fasta" from_work_dir="out/snps.aligned.fa"/>
    <data format="fasta" name="snpconsensus" label="${tool.name} on ${on_string} consensus fasta" from_work_dir="out/snps.consensus.fa"/>
    <data format="tabular" name="snpsdepth" label="${tool.name} on ${on_string} mapping depth" from_work_dir="out/snps.depth"/>
    <data format="bam" name="snpsbam" label="${tool.name} on ${on_string} mapped reads (bam)" from_work_dir="out/snps.bam">
      <filter>cleanup is False</filter>
    </data>
    <data format="zip" name="outdir" label="${tool.name} on ${on_string} out dir" from_work_dir="out.tgz" />
  </outputs>

  <tests>
    <test>
      <param name="ref_type_selector" value="fasta" />
      <param name="ref" value="Ecoli.fna" ftype="fasta" />
      <param name="fastq_input_selector" value="paired" />
      <param name="fastq_input1" ftype="fastq" value="reads_1.fq" />
      <param name="fastq_input2" ftype="fastq" value="reads_2.fq" />
      <output name="snpsum" ftype="tabular" file="test/snps.txt" lines-diff="5" />
    </test>
  </tests>


  <help>
    <![CDATA[
Synopsis:
  snippy 3.0 - fast bacterial variant calling from NGS reads

Author:
  Torsten Seemann <[e-mail]>

Usage:
  snippy [options] --outdir <dir> --ref <ref> --pe1 <R1.fq.gz> --pe2 <R2.fq.gz>

  snippy [options] --outdir <dir> --ref <ref> --se <454.fastq>

  snippy [options] --outdir <dir> --ref <ref> --peil <velvet.fa.gz>

Options:
  --help            This help

  --version         Print version and exit

  --citation        Print citation for referencing snippy

  --quiet           No screen output (default OFF)

  --cpus [N]        Maximum number of CPU cores to use (default '8')

  --reference [X]   Reference genome. Supports FASTA, GenBank, EMBL (not GFF) (default '')

  --outdir [X]      Output folder (default '')

  --prefix [X]      Prefix for output files (default 'snps')

  --force           Force overwrite of existing output folder (default OFF)

  --pe1|R1|left [X] Reads, paired-end R1 (left) (default '')

  --pe2|R2|right [X] Reads, paired-end R2 (right) (default '')

  --se|single [X]   Single-end reads (default '')

  --peil [X]        Reads, paired-end R1/R2 interleaved (default '')

  --mapqual [n.n]   Minimum mapping quality to allow (default '60')

  --mincov [N]      Minimum coverage of variant site (default '10')

  --minfrac [n.n]   Minumum proportion for variant evidence (default '0.9')

  --report          Produce long report with visual alignment (slow) (default OFF)

  --cleanup         Remove all non-SNP files: BAMs, indices etc (default OFF)

  --rgid [X]        Use this @RG ID: in the BAM header (default '')

  --bwaopt [X]      Extra BWA MEM options, eg. -x pacbio (default '')

    ]]>
  </help>
  <citations>
    <citation type="bibtex">
      @UNPUBLISHED{Seemann2013,
      author = "Seemann T",
      title = "snippy: fast bacterial variant calling from NGS reads",
      year = "2015",
      note = "https://github.com/tseemann/snippy"}
    </citation>
  </citations>


</tool>