# HG changeset patch
# User jjjjia
# Date 1535244997 14400
# Node ID a14b12a71a53baf66866c408d492a1b8754d365b
# Parent 4b2738bc81ed7840123702ad0116103cbfb9af4f
planemo upload
diff -r 4b2738bc81ed -r a14b12a71a53 cpo_combine.sh
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/cpo_combine.sh Sat Aug 25 20:56:37 2018 -0400
@@ -0,0 +1,9 @@
+head -1 ${1[1]} > combined.tsv
+
+IFS=',' read -ra ADDR <<< "$1" #hax to read in a csv
+
+head -1 ${ADDR[0]} > ./combined.tsv
+for i in "${ADDR[@]}"; do
+ echo $i
+ tail -1 $i >> ./combined.tsv
+done
diff -r 4b2738bc81ed -r a14b12a71a53 cpo_combiner.sh
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/cpo_combiner.sh Sat Aug 25 20:56:37 2018 -0400
@@ -0,0 +1,11 @@
+#!/bin/bash
+
+head -1 ${1[1]} > combined.tsv
+
+IFS=',' read -ra ADDR <<< "$1" #hax to read in a csv
+
+head -1 ${ADDR[0]} > ./combined.tsv
+for i in "${ADDR[@]}"; do
+ echo $i
+ tail -1 $i >> ./combined.tsv
+done
\ No newline at end of file
diff -r 4b2738bc81ed -r a14b12a71a53 cpo_galaxy_prediction.py
--- a/cpo_galaxy_prediction.py Fri Aug 24 19:10:42 2018 -0400
+++ b/cpo_galaxy_prediction.py Sat Aug 25 20:56:37 2018 -0400
@@ -457,7 +457,10 @@
pf.start = int(plasmidFinder.iloc[i,2])
pf.end = int(plasmidFinder.iloc[i,3])
pf.gene = str(plasmidFinder.iloc[i,4])
- pf.shortGene = pf.gene[:pf.gene.index("_")]
+ if (pf.gene.find("_") > -1):
+ pf.shortGene = pf.gene[:pf.gene.index("_")]
+ else:
+ pf.shortGene = pf.gene
pf.coverage = str(plasmidFinder.iloc[i,5])
pf.coverage_map = str(plasmidFinder.iloc[i,6])
pf.gaps = str(plasmidFinder.iloc[i,7])
@@ -528,12 +531,14 @@
rgiAMR = ParseRGIResult(rgi, plasmidContigs, likelyPlasmidContigs) # outputDir + "/predictions/" + ID + ".rgi.txt", plasmidContigs, likelyPlasmidContigs)#***********************
ToJson(rgiAMR, "rgi.json") #*************
- carbapenamases = []
+ carbapenamases = []
+ resfinderCarbas = [] #list of rfinder objects for lindaout list
amrGenes = []
for keys in rFinder:
carbapenamases.append(rFinder[keys].shortGene + "(" + rFinder[keys].source + ")")
+ resfinderCarbas.append(rFinder[keys])
for keys in rgiAMR:
- if (rgiAMR[keys].Drug_Class.find("carbapenem") > -1):
+ if (rgiAMR[keys].Drug_Class.find("carbapenem") > -1 and rgiAMR[keys].AMR_Gene_Family.find("beta-lactamase") > -1):
if (rgiAMR[keys].Best_Hit_ARO not in carbapenamases):
carbapenamases.append(rgiAMR[keys].Best_Hit_ARO+ "(" + rgiAMR[keys].source + ")")
else:
@@ -598,7 +603,28 @@
#TSV output
lindaOut = []
tsvOut = []
- lindaOut.append("new\tID\tQUALITY\tExpected Species\tMLST Scheme\tSequence Type\tMLST_ALLELE_1\tMLST_ALLELE_2\tMLST_ALLELE_3\tMLST_ALLELE_4\tMLST_ALLELE_5\tMLST_ALLELE_6\tMLST_ALLELE_7\tSEROTYPE\tK_CAPSULE\tPLASMID_1_FAMILY\tPLASMID_1_BEST_MATCH\tPLASMID_1_COVERAGE\tPLASMID_1_SNVS_TO_BEST_MATCH\tPLASMID_1_CARBAPENEMASE\tPLASMID_1_INC_GROUP\tPLASMID_2_RFLP\tPLASMID_2_FAMILY\tPLASMID_2_BEST_MATCH\tPLASMID_2_COVERAGE\tPLASMID_2_SNVS_TO_BEST_MATCH\tPLASMID_2_CARBAPENEMASE\tPLASMID_2_INC_GROUP")
+ lindaOut.append("ID\tQUALITY\tExpected Species\tMLST Scheme\tSequence Type\tMLST_ALLELE_1\tMLST_ALLELE_2\tMLST_ALLELE_3\tMLST_ALLELE_4\tMLST_ALLELE_5\tMLST_ALLELE_6\tMLST_ALLELE_7\tSEROTYPE\tK_CAPSULE\tPLASMID_2_RFLP\tPLASMID_1_FAMILY\tPLASMID_1_BEST_MATCH\tPLASMID_1_COVERAGE\tPLASMID_1_SNVS_TO_BEST_MATCH\tPLASMID_1_CARBAPENEMASE\tPLASMID_1_INC_GROUP\tPLASMID_2_RFLP\tPLASMID_2_FAMILY\tPLASMID_2_BEST_MATCH\tPLASMID_2_COVERAGE\tPLASMID_2_SNVS_TO_BEST_MATCH\tPLASMID_2_CARBAPENEMASE\tPLASMID_2_INC_GROUP")
+ lindaTemp = ID + "\t" #id
+ lindaTemp += "\t" #quality
+ lindaTemp += expectedSpecies + "\t" #expected
+ lindaTemp += mlstHit.species + "\t" #mlstscheme
+ lindaTemp += str(mlstHit.seqType) + "\t" #seq type
+ lindaTemp += "\t".join(mlstHit.scheme.split(";")) + "\t"#mlst alleles x 7
+ lindaTemp += "\t\t" #sero and kcap
+
+ #resfinderCarbas
+ for carbs in resfinderCarbas:
+ if (carbs.source == "plasmid"): #
+ lindaTemp += "\t\t\t\t\t" #plasmid 1 rflp plasmid 1 family information. PLASMID_1_FAMILY\tPLASMID_1_BEST_MATCH\tPLASMID_1_COVERAGE\tPLASMID_1_SNVS_TO_BEST_MATCH
+ lindaTemp += carbs.shortGene + "\t" #found an carbapenase
+ contig = carbs.sequence[6:] #this is the contig number
+ for i in mSuite.keys():
+ if (str(mSuite[i].contig_num) == str(contig)): #found the right plasmid
+ lindaTemp += mSuite[i].rep_type
+ lindaOut.append(lindaTemp)
+ out = open("summary.linda.tsv", 'w')
+ for item in lindaOut:
+ out.write("%s\n" % item)
tsvOut.append("new\tID\tExpected Species\tMLST Species\tSequence Type\tMLST Scheme\tCarbapenem Resistance Genes\tOther AMR Genes\tTotal Plasmids\tPlasmids ID\tNum_Contigs\tPlasmid Length\tPlasmid RepType\tPlasmid Mobility\tNearest Reference\tDefinitely Plasmid Contigs\tLikely Plasmid Contigs")
#start with ID
diff -r 4b2738bc81ed -r a14b12a71a53 cpo_galaxy_predictions.xml
--- a/cpo_galaxy_predictions.xml Fri Aug 24 19:10:42 2018 -0400
+++ b/cpo_galaxy_predictions.xml Sat Aug 25 20:56:37 2018 -0400
@@ -26,10 +26,11 @@
-
+
+
diff -r 4b2738bc81ed -r a14b12a71a53 cpo_galaxy_tree.py
--- a/cpo_galaxy_tree.py Fri Aug 24 19:10:42 2018 -0400
+++ b/cpo_galaxy_tree.py Sat Aug 25 20:56:37 2018 -0400
@@ -263,7 +263,8 @@
n.add_face(addFace(mData.CarbapenemResistanceGenes), index, "aligned")
index = index + 1
for i in range(len(distanceDict[list(distanceDict.keys())[0]])): #this loop adds distance matrix
- n.add_face(addFace(list(distanceDict[n.name])[i]), index + i, "aligned")
+ if (n.name in distanceDict): #make sure the column is in the distance matrice
+ n.add_face(addFace(list(distanceDict[n.name])[i]), index + i, "aligned")
t.render("./tree.pdf", w=5000,units="mm", tree_style=ts) #save it as a png. or an phyloxml
diff -r 4b2738bc81ed -r a14b12a71a53 cpo_prediction_combine.xml
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/cpo_prediction_combine.xml Sat Aug 25 20:56:37 2018 -0400
@@ -0,0 +1,31 @@
+
+ This tool combines a collection result from cpo_prediction_parser into 1 file
+
+ python
+
+
+
+
+
+
+
+
+
+
+
+ This tool combines multiple single prediction outputs together into one.
+
+
+
+@misc{cpo,
+ author = {j, j},
+ year = {2018},
+ title = {cpo_prediction},
+ publisher = {j},
+ journal = {j of j},
+ url = {https://bfjia.net,
+}
+
+
\ No newline at end of file