Mercurial > repos > jjkoehorst > sapp
annotate crt.xml @ 35:fa736576c7ed draft
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author | jjkoehorst |
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date | Mon, 04 Jul 2016 10:37:59 -0400 |
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1 <tool id="DCRT" name="CRISPR detection" version="0.1"> |
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2 <description></description> |
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3 <requirements> |
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4 <container type="docker">jjkoehorst/sappdocker:CRT</container> |
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5 </requirements> |
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6 <command interpreter="docker">java -jar /crt/CRT-0.0.1-SNAPSHOT-jar-with-dependencies.jar |
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7 '-input' '$input' -output '$output' -format TURTLE |
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8 </command> |
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9 <inputs> |
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10 <param name="input" type="data" format="ttl" label="genome ttl file" /> |
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11 </inputs> |
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12 |
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13 <outputs> |
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14 <data format="ttl" name="output" label="CRISPR: ${input.name}" /> |
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15 </outputs> |
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16 <help> |
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17 CIRSPR prediction using CRT. Requires a converted |
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18 FASTA/EMBL/GenBank file. |
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19 </help> |
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20 <citations> |
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21 <citation type="bibtex"> |
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22 @article{Bland2007, |
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23 abstract = {BACKGROUND: |
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24 Clustered Regularly Interspaced Palindromic Repeats |
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25 (CRISPRs) are a |
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26 novel type of direct repeat found in a wide range of |
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27 bacteria and |
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28 archaea. CRISPRs are beginning to attract attention |
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29 because of their |
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30 proposed mechanism; that is, defending their hosts |
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31 against invading |
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32 extrachromosomal elements such as viruses. Existing |
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33 repeat detection |
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34 tools do a poor job of identifying CRISPRs due to |
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35 the presence of |
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36 unique spacer sequences separating the repeats. In |
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37 this study, a new |
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38 tool, CRT, is introduced that rapidly and |
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39 accurately identifies |
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40 CRISPRs in large DNA strings, such as genomes |
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41 and metagenomes. |
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42 RESULTS: CRT was compared to CRISPR detection tools, |
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43 Patscan and |
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44 Pilercr. In terms of correctness, CRT was shown to be |
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45 very reliable, |
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46 demonstrating significant improvements over Patscan |
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47 for measures |
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48 precision, recall and quality. When compared to Pilercr, |
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49 CRT showed |
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50 improved performance for recall and quality. In terms of |
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51 speed, CRT |
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52 proved to be a huge improvement over Patscan. Both CRT and |
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53 Pilercr |
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54 were comparable in speed, however CRT was faster for genomes |
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55 containing large numbers of repeats. CONCLUSION: In this paper a new |
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56 tool was introduced for the automatic detection of CRISPR elements. |
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57 This tool, CRT, showed some important improvements over current |
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58 techniques for CRISPR identification. CRT's approach to detecting |
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59 repetitive sequences is straightforward. It uses a simple sequential |
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60 scan of a DNA sequence and detects repeats directly without any major |
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61 conversion or preprocessing of the input. This leads to a program |
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62 that is easy to describe and understand; yet it is very accurate, |
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63 fast and memory efficient, being O(n) in space and O(nm/l) in time.}, |
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64 author = {Bland, Charles and Ramsey, Teresa L and Sabree, Fareedah |
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65 and Lowe, Micheal and Brown, Kyndall and Kyrpides, Nikos C and |
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66 Hugenholtz, Philip}, |
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67 doi = {10.1186/1471-2105-8-209}, |
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68 file = |
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69 {:Users/koeho006/Library/Application Support/Mendeley |
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70 Desktop/Downloaded/Bland et al. - 2007 - CRISPR recognition tool |
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71 (CRT) a tool for automatic detection of clustered regularly |
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72 interspaced palindromic repeat.pdf:pdf}, |
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73 isbn = {1471-2105 |
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74 (Electronic)$\backslash$n1471-2105 (Linking)}, |
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75 issn = {14712105}, |
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76 journal = {BMC bioinformatics}, |
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77 mendeley-groups = {VAPP Application |
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78 note}, |
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79 pages = {209}, |
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80 pmid = {17577412}, |
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81 title = {{CRISPR recognition |
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82 tool (CRT): a tool for automatic detection of |
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83 clustered regularly |
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84 interspaced palindromic repeats.}}, |
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85 volume = {8}, |
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86 year = {2007} |
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87 } |
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88 </citation> |
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89 </citations> |
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90 </tool> |