# HG changeset patch
# User jjohnson
# Date 1633691781 0
# Node ID 9f2665b32c454527121cded659e4bb096e3ac814
# Parent 5ebf2354cc9b7faaeed4460d1ac6aaff933f650a
"planemo upload for repository https://github.com/jj-umn/tools-iuc/tree/arriba/tools/arriba commit 933ae7dfba10b1b31c30a90216d76cdad6dda685"
diff -r 5ebf2354cc9b -r 9f2665b32c45 arriba.help
--- a/arriba.help Thu Oct 07 11:47:02 2021 +0000
+++ /dev/null Thu Jan 01 00:00:00 1970 +0000
@@ -1,191 +0,0 @@
-% arriba -h
-[2021-10-06T19:04:33] Launching Arriba 2.1.0
-
-Arriba gene fusion detector
----------------------------
-Version: 2.1.0
-
-Arriba is a fast tool to search for aberrant transcripts such as gene fusions.
-It is based on chimeric alignments found by the STAR RNA-Seq aligner.
-
-Usage: arriba [-c Chimeric.out.sam] -x Aligned.out.bam \
- -g annotation.gtf -a assembly.fa [-b blacklists.tsv] [-k known_fusions.tsv] \
- [-t tags.tsv] [-p protein_domains.gff3] [-d structural_variants_from_WGS.tsv] \
- -o fusions.tsv [-O fusions.discarded.tsv] \
- [OPTIONS]
-
- -c FILE File in SAM/BAM/CRAM format with chimeric alignments as generated by STAR
- (Chimeric.out.sam). This parameter is only required, if STAR was run with the
- parameter '--chimOutType SeparateSAMold'. When STAR was run with the parameter
- '--chimOutType WithinBAM', it suffices to pass the parameter -x to Arriba and -c
- can be omitted.
-
- -x FILE File in SAM/BAM/CRAM format with main alignments as generated by STAR
- (Aligned.out.sam). Arriba extracts candidate reads from this file.
-
- -g FILE GTF file with gene annotation. The file may be gzip-compressed.
-
- -G GTF_FEATURES Comma-/space-separated list of names of GTF features.
- Default: gene_name=gene_name|gene_id gene_id=gene_id
- transcript_id=transcript_id feature_exon=exon feature_CDS=CDS
-
- -a FILE FastA file with genome sequence (assembly). The file may be gzip-compressed. An
- index with the file extension .fai must exist only if CRAM files are processed.
-
- -b FILE File containing blacklisted events (recurrent artifacts and transcripts
- observed in healthy tissue).
-
- -k FILE File containing known/recurrent fusions. Some cancer entities are often
- characterized by fusions between the same pair of genes. In order to boost
- sensitivity, a list of known fusions can be supplied using this parameter. The list
- must contain two columns with the names of the fused genes, separated by tabs.
-
- -o FILE Output file with fusions that have passed all filters.
-
- -O FILE Output file with fusions that were discarded due to filtering.
-
- -t FILE Tab-separated file containing fusions to annotate with tags in the 'tags' column.
- The first two columns specify the genes; the third column specifies the tag. The
- file may be gzip-compressed.
-
- -p FILE File in GFF3 format containing coordinates of the protein domains of genes. The
- protein domains retained in a fusion are listed in the column
- 'retained_protein_domains'. The file may be gzip-compressed.
-
- -d FILE Tab-separated file with coordinates of structural variants found using
- whole-genome sequencing data. These coordinates serve to increase sensitivity
- towards weakly expressed fusions and to eliminate fusions with low evidence.
-
- -D MAX_GENOMIC_BREAKPOINT_DISTANCE When a file with genomic breakpoints obtained via
- whole-genome sequencing is supplied via the -d
- parameter, this parameter determines how far a
- genomic breakpoint may be away from a
- transcriptomic breakpoint to consider it as a
- related event. For events inside genes, the
- distance is added to the end of the gene; for
- intergenic events, the distance threshold is
- applied as is. Default: 100000
-
- -s STRANDEDNESS Whether a strand-specific protocol was used for library preparation,
- and if so, the type of strandedness (auto/yes/no/reverse). When
- unstranded data is processed, the strand can sometimes be inferred from
- splice-patterns. But in unclear situations, stranded data helps
- resolve ambiguities. Default: auto
-
- -i CONTIGS Comma-/space-separated list of interesting contigs. Fusions between genes
- on other contigs are ignored. Contigs can be specified with or without the
- prefix "chr". Asterisks (*) are treated as wild-cards.
- Default: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 X Y AC_* NC_*
-
- -v CONTIGS Comma-/space-separated list of viral contigs. Asterisks (*) are treated as
- wild-cards.
- Default: AC_* NC_*
-
- -f FILTERS Comma-/space-separated list of filters to disable. By default all filters are
- enabled. Valid values: homologs, low_entropy, isoforms,
- top_expressed_viral_contigs, viral_contigs, non_coding_neighbors,
- mismatches, duplicates, no_genomic_support, genomic_support, intronic,
- end_to_end, relative_support, low_coverage_viral_contigs,
- merge_adjacent, mismappers, multimappers, same_gene, long_gap,
- internal_tandem_duplication, small_insert_size, read_through,
- inconsistently_clipped, uninteresting_contigs, intragenic_exonic,
- spliced, hairpin, blacklist, min_support, select_best, in_vitro,
- short_anchor, known_fusions, no_coverage, homopolymer, many_spliced
-
- -E MAX_E-VALUE Arriba estimates the number of fusions with a given number of supporting
- reads which one would expect to see by random chance. If the expected number
- of fusions (e-value) is higher than this threshold, the fusion is
- discarded by the 'relative_support' filter. Note: Increasing this
- threshold can dramatically increase the number of false positives and may
- increase the runtime of resource-intensive steps. Fractional values are
- possible. Default: 0.300000
-
- -S MIN_SUPPORTING_READS The 'min_support' filter discards all fusions with fewer than
- this many supporting reads (split reads and discordant mates
- combined). Default: 2
-
- -m MAX_MISMAPPERS When more than this fraction of supporting reads turns out to be
- mismappers, the 'mismappers' filter discards the fusion. Default:
- 0.800000
-
- -L MAX_HOMOLOG_IDENTITY Genes with more than the given fraction of sequence identity are
- considered homologs and removed by the 'homologs' filter.
- Default: 0.300000
-
- -H HOMOPOLYMER_LENGTH The 'homopolymer' filter removes breakpoints adjacent to
- homopolymers of the given length or more. Default: 6
-
- -R READ_THROUGH_DISTANCE The 'read_through' filter removes read-through fusions
- where the breakpoints are less than the given distance away
- from each other. Default: 10000
-
- -A MIN_ANCHOR_LENGTH Alignment artifacts are often characterized by split reads coming
- from only one gene and no discordant mates. Moreover, the split
- reads only align to a short stretch in one of the genes. The
- 'short_anchor' filter removes these fusions. This parameter sets
- the threshold in bp for what the filter considers short. Default: 23
-
- -M MANY_SPLICED_EVENTS The 'many_spliced' filter recovers fusions between genes that
- have at least this many spliced breakpoints. Default: 4
-
- -K MAX_KMER_CONTENT The 'low_entropy' filter removes reads with repetitive 3-mers. If
- the 3-mers make up more than the given fraction of the sequence, then
- the read is discarded. Default: 0.600000
-
- -V MAX_MISMATCH_PVALUE The 'mismatches' filter uses a binomial model to calculate a
- p-value for observing a given number of mismatches in a read. If
- the number of mismatches is too high, the read is discarded.
- Default: 0.010000
-
- -F FRAGMENT_LENGTH When paired-end data is given, the fragment length is estimated
- automatically and this parameter has no effect. But when single-end
- data is given, the mean fragment length should be specified to
- effectively filter fusions that arise from hairpin structures.
- Default: 200
-
- -U MAX_READS Subsample fusions with more than the given number of supporting reads. This
- improves performance without compromising sensitivity, as long as the
- threshold is high. Counting of supporting reads beyond the threshold is
- inaccurate, obviously. Default: 300
-
- -Q QUANTILE Highly expressed genes are prone to produce artifacts during library
- preparation. Genes with an expression above the given quantile are eligible
- for filtering by the 'in_vitro' filter. Default: 0.998000
-
- -e EXONIC_FRACTION The breakpoints of false-positive predictions of intragenic events
- are often both in exons. True predictions are more likely to have at
- least one breakpoint in an intron, because introns are larger. If the
- fraction of exonic sequence between two breakpoints is smaller than
- the given fraction, the 'intragenic_exonic' filter discards the
- event. Default: 0.330000
-
- -T TOP_N Only report viral integration sites of the top N most highly expressed viral
- contigs. Default: 5
-
- -C COVERED_FRACTION Ignore virally associated events if the virus is not fully
- expressed, i.e., less than the given fraction of the viral contig is
- transcribed. Default: 0.150000
-
- -l MAX_ITD_LENGTH Maximum length of internal tandem duplications. Note: Increasing
- this value beyond the default can impair performance and lead to many
- false positives. Default: 100
-
- -u Instead of performing duplicate marking itself, Arriba relies on duplicate marking by a
- preceding program using the BAM_FDUP flag. This makes sense when unique molecular
- identifiers (UMI) are used.
-
- -X To reduce the runtime and file size, by default, the columns 'fusion_transcript',
- 'peptide_sequence', and 'read_identifiers' are left empty in the file containing
- discarded fusion candidates (see parameter -O). When this flag is set, this extra
- information is reported in the discarded fusions file.
-
- -I If assembly of the fusion transcript sequence from the supporting reads is incomplete
- (denoted as '...'), fill the gaps using the assembly sequence wherever possible.
-
- -h Print help and exit.
-
- Code repository: https://github.com/suhrig/arriba
- Get help/report bugs: https://github.com/suhrig/arriba/issues
- User manual: https://arriba.readthedocs.io/
- Please cite: https://doi.org/10.1101/gr.257246.119
-
diff -r 5ebf2354cc9b -r 9f2665b32c45 arriba.xml
--- a/arriba.xml Thu Oct 07 11:47:02 2021 +0000
+++ b/arriba.xml Fri Oct 08 11:16:21 2021 +0000
@@ -6,31 +6,95 @@
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diff -r 5ebf2354cc9b -r 9f2665b32c45 arriba_download_reference.xml
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/arriba_download_reference.xml Fri Oct 08 11:16:21 2021 +0000
@@ -0,0 +1,71 @@
+
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+ macros.xml
+
+
+
+ '$arriba_reference'
+ mkdir -p '$arriba_reference.files_path' &&
+ cd '$arriba_reference.files_path' &&
+ BASE_DIR=$(dirname $(dirname `which arriba`)) &&
+ REF_SCRIPT=`find $BASE_DIR -name 'download_references.sh'` &&
+ $REF_SCRIPT '$arriba_reference_name'
+ ]]>
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diff -r 5ebf2354cc9b -r 9f2665b32c45 test-data/Aligned.out.sam
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/Aligned.out.sam Fri Oct 08 11:16:21 2021 +0000
@@ -0,0 +1,111 @@
+@HD VN:1.4 SO:coordinate
+@SQ SN:1 LN:248956422
+@SQ SN:2 LN:242193529
+@SQ SN:3 LN:198295559
+@SQ SN:4 LN:190214555
+@SQ SN:5 LN:181538259
+@SQ SN:6 LN:170805979
+@SQ SN:7 LN:159345973
+@SQ SN:8 LN:145138636
+@SQ SN:9 LN:138394717
+@SQ SN:10 LN:133797422
+@SQ SN:11 LN:135086622
+@SQ SN:12 LN:133275309
+@SQ SN:13 LN:114364328
+@SQ SN:14 LN:107043718
+@SQ SN:15 LN:101991189
+@SQ SN:16 LN:90338345
+@SQ SN:17 LN:83257441
+@SQ SN:18 LN:80373285
+@SQ SN:19 LN:58617616
+@SQ SN:20 LN:64444167
+@SQ SN:21 LN:46709983
+@SQ SN:22 LN:50818468
+@SQ SN:X LN:156040895
+@SQ SN:Y LN:57227415
+@SQ SN:MT LN:16569
+@PG ID:STAR PN:STAR VN:2.7.8a CL:STAR --runThreadN 12 --genomeDir /panfs/roc/website/galaxy.msi.umn.edu/galaxy/tool-data/rnastar/2.7.4a/GRCh38_canon/GRCh38_canon/dataset_1367616_files --genomeLoad NoSharedMemory --readFilesIn /panfs/roc/galaxy/PRODUCTION/database/files/001/368/dataset_1368710.dat /panfs/roc/galaxy/PRODUCTION/database/files/001/368/dataset_1368711.dat --readFilesCommand zcat --limitBAMsortRAM 122880000000 --outSAMtype BAM SortedByCoordinate --outSAMstrandField intronMotif --outSAMattributes NH HI AS nM ch --outSAMunmapped Within --outSAMprimaryFlag OneBestScore --outSAMmapqUnique 60 --outBAMsortingThreadN 12 --outBAMsortingBinsN 50 --outSAMattrIHstart 1 --winAnchorMultimapNmax 50 --chimSegmentMin 12 --chimOutType WithinBAM Junctions --chimOutJunctionFormat 1 --quantMode GeneCounts --twopass1readsN 50000 --twopassMode Basic
+@CO user command line: STAR --runThreadN 12 --genomeLoad NoSharedMemory --genomeDir /panfs/roc/website/galaxy.msi.umn.edu/galaxy/tool-data/rnastar/2.7.4a/GRCh38_canon/GRCh38_canon/dataset_1367616_files --readFilesIn /panfs/roc/galaxy/PRODUCTION/database/files/001/368/dataset_1368710.dat /panfs/roc/galaxy/PRODUCTION/database/files/001/368/dataset_1368711.dat --readFilesCommand zcat --outSAMtype BAM SortedByCoordinate --twopassMode Basic --twopass1readsN 50000 --quantMode GeneCounts --outSAMstrandField intronMotif --outSAMattrIHstart 1 --outSAMattributes NH HI AS nM ch --outSAMprimaryFlag OneBestScore --outSAMmapqUnique 60 --outSAMunmapped Within --chimSegmentMin 12 --outBAMsortingThreadN 12 --outBAMsortingBinsN 50 --winAnchorMultimapNmax 50 --limitBAMsortRAM 122880000000 --chimOutType WithinBAM Junctions --chimOutJunctionFormat 1
+BCR-ABL1-76 99 9 130854061 60 24S126M = 130854103 755 CAGCCACTGGATTTAAGCAGAGTTCAAAAGCCCTTCAGCGGCCAGTAGCATCTGACTTTGAGCCTCAGGGTCTGAGTGAAGCCGCTCGTTGGAACTCCAAGGAAAACCTTCTCGCTGGACCCAGTGAAAATGACCCCAACCTTTTCGTTG CCCGGGGCGGGCGJJJJJGJJJGJJJJJJJJJGJ1JCJJGJGGJJJGJJGGJJJ8GGJJGGGJJ=GGCGGGGGG=GGCCGGG8GC=GGGG=GCGGCGGGGJGG=GGGG=GGGGGGGGCGGGGCCGGGCG=GG(G=GCGCCG1CCGGCGGG NH:i:1 HI:i:1 AS:i:274 nM:i:1 XS:A:+ NM:i:1
+BCR-ABL1-64 99 9 130854061 60 6S144M = 130854104 756 AGAGTTCAAAAGCCCTTCAGCGGCCAGTAGCATCTGACTTTGAGCCTCAGGGTCTGAGTGAAGCCGCTCGTTGGAACTCCAAGGAAAACCTTCTCGCTGGACCCAGTGAAAATGACCCCAACCTTTTCGTTGCACTGTATGATTTTGTGG CCCGGGGGCGGGGGJJJGJJJGGJJJJJCJJJJGGJJJGJJGJGJG=GGJG=JJJJCGCCC==JGGCGGGCJG1CCCCGG8CGGGGGGGGCCGC=CGCGGJGGGGCGCGGGGGGGGCCGCGGGG=GCGGGGGGG=GGGGCGGGGGGCCGG NH:i:1 HI:i:1 AS:i:290 nM:i:2 XS:A:+ NM:i:1
+BCR-ABL1-54 99 9 130854061 60 61S89M = 130854061 140 CCGGGGCTCTATGGGTTTCTGAATGTCATCGTCCACTCAGCCACTGGATTTAAGCAGAGTTCAAAAGCCCTTGAGCGGCCAGTAGCATCTGACTTTGAGCCTCAGGGTCTGAGTGAAGCCGCTCGTTGGAACTCCAAGGAAAACCTTCTC CCCGGGGGGGGGGGJJJJJGJ=JJJJJJGJJJGGJJJJJJJCJJG8JJJGJJGJ=GG=JJJGGCGGCGGJGC(GGGGGCGC8CGGCGCCGGC=GGGCGGGJG1GGGGGG1CG=GGGGC=1G1CGGGGGCCGGGGCGG=CC=C=CGGGGG8 NH:i:1 HI:i:1 AS:i:219 nM:i:4 NM:i:2
+BCR-ABL1-54 147 9 130854061 60 10S140M = 130854061 -140 AAGCAGAGTTCAAAAGCCCTTCAGCGGCCAGTAGCATCTGACTTTGAGCCTCAGGGTCTGAGTGAAGCCGCTCGTTGGAACTCCAAGGAAAACGTTCTCGCTGGACCCAGTGAAAATGACCCCAACCTTTTCGTTGCACTGTATGATTTT =GGGGGGGCCCCGCCGGG=G(GGGG=CGCGGCGCCGG=GGGGGCJJJ=GC8C1GGGGGCG8GCCGC=GGG1GCCGGJC8GCGGCGCGJGJJJG1CGJGG=CJJJGGGGJG=CJGJJJJCJCJJGGJJJJJGGJGGJJCGGGGGGGGG=CC NH:i:1 HI:i:1 AS:i:219 nM:i:4 NM:i:2
+BCR-ABL1-48 163 9 130854061 60 3S147M = 130854101 753 GTTCAAAAGCCCTTCAGCGGCCAGTAGCATCTGACTTTGAGCCTCAGGGTCTGAGTGAAGCCGCTCGTTGGAACTCCAAGGAAAACCTTCTCGCTGGACCCAGTGAAAATGACCCCAACCTTTTCGTTGCACTGTATGATTTTGTGGCCA CCCGGGCGGGGGGGJ1JJJJJJJCJJJCGJJCGGJCJJGJGGJJJGGGCGJJ=GJJJG=JCG=GJGGGC8=GCG=G=GCCGGG1CG1GC=GGG8GGGGG1GCJJCJJCCGGCGCCG=CGCGGGCGG=GCGGG1CGC1CGC=CGGGCGGGG NH:i:1 HI:i:1 AS:i:295 nM:i:1 XS:A:+ NM:i:1
+BCR-ABL1-28 163 9 130854061 60 19S131M = 130854092 744 ACTGGATTTAAGCAGAGTTCAAAAGCCCTTCAGCGGCCAGTAGCATCTGACTTTGAGCCCCAGGGTCTGAGTGAAGCCGCTCGTTGGAACTCCAAGGAAAACCTTCTCGCTGGACCCAGTGAAAATGACCCCAACCTTTTCGTTGCACTG CCCGCGGGGGGGGGJ=GJCGJJJ1JCJJJJGJJJJJCCJJJJG8JJCC=CGGCGGJGGC(JGGG=GCCGCGCJ8CGGG=GGGCGGGGGCGGCCGGCCGGGCCJ=JC=CGCGCGC1G8GCCGGGGGC=GCGGGCGGGGGGGGGGGGGCGCC NH:i:1 HI:i:1 AS:i:275 nM:i:3 XS:A:+ NM:i:2
+BCR-ABL1-2 99 9 130854061 60 62S88M = 130854061 134 TCCGGGGCTCTATGGGTTTCTGAATGTCATCGTCCACTCAGCCACTGGATTTAAGCAGAGTTCAAAAGCCCCTCAGCGGCCAGTAGCATCTGACTTTGAGCCTCAGGGTCTGAGTGAAGCCGCTCGTTGGAACTCCAAGGAAAACCTTCT CCCGGGGGGGGGGGJ1JJJJJJ=JGGGGJJJCGCJJJCJJJJGGCJGGGJCJGGJJGJCGJGG1GCG=CGG(G=CGGG1GGCCGGGGGGGCGGGG=GCCGJGGCGCGGGGCCCG1GGGCCGGG8GGCGGCGG=CC(G=GC1GGCCGGGCG NH:i:1 HI:i:1 AS:i:214 nM:i:3 NM:i:2
+BCR-ABL1-2 147 9 130854061 60 16S134M = 130854061 -134 GGATTTAAGCAGAGTTCAAAAGCCCTTCAGCGGCCAGTAGCATCTGACTTTGAGCCTCAGGGTCTGAGTGAAGCCGCTCGTTGGAACTCCAAGGAAAACCTTCTCGCTGGACCCAGTGAAAATGACCCCAACCTTTTCGTTGCACTGTAT CGGGC=GGGGG=GGGGGGCCCCG=GG8=8CCGCGGGGGCGCGCJJCJ=CCCCC81GGGGC=GGGC8C8GGCGCGJCCGG8JCCGCC1GGCGGCJGGGJJJGGJJJJJCGGJGCJJJJJJG=JGJGJJJJGJJJGGJGCGCGGGGGGGCCC NH:i:1 HI:i:1 AS:i:214 nM:i:3 NM:i:1
+BCR-ABL1-68 99 9 130854064 60 1S149M = 130854089 175 AAAGCCCTTCAGCGGCCAGTAGCATCTGACTTTGAGCCTCAGGGTCTGAGTGAAGCCGCTCGTTGGAACTCCAAGGAAAACCTTCTCGCTGGACCCAGTGAAAATGACCCCAACCTTTTCGTTGCACTGTATGATTTTGTGGCCAGTGGA CC1=GC=GGGGGGJJJJGJJJJJGGJJJJJJJGJJJJJ=(GJGGG8CCGJJ=GJGGGGJGJGJ=GGGCCGCGGCG1CGCGGGGGGGCGCCGCGGCGGGGGJGCC8GCGGGGCGGC=GGGGG=GGGCCC=GCGGGGGGCGCGGGCGGCGCG NH:i:1 HI:i:1 AS:i:291 nM:i:3 NM:i:0
+BCR-ABL1-60 99 9 130854064 60 39S111M = 130854074 160 TCATCGTCCACTCAGCCACTGGATTTAAGCAGAGTTCAAAAGCCCTTCAGCGGCCAGTAGCATCTGACTTTGAGCCTCAGGGTCTGAGTGAAGCCGCTCGTTGGAACTCCAAGGAAAACCTTCTCGCTGGACCCAGTGAAAATGACCCCA =CCGGCGGGGG=GJJGJGGGCJJCJJGJCGJG(J(JJJGGCGGGJJJGCJGGG1G=JGGJJGCJCCGGJ(JJCCGCC=GCGGGCGGGGG1GGGGCGCGG(JCGCGGGGGGGGGGGGCCGGCGCGCGGGGGGGCGGGGCGG1GGGGGGCGC NH:i:1 HI:i:1 AS:i:259 nM:i:0 ch:A:1 NM:i:0 SA:Z:22,23290375,+,39M111H,60,0;
+BCR-ABL1-18 2193 9 130854064 60 118H32M 22 23289532 0 AAGCCCTTCAGCGGCCAGTAGCATCTGACTTT JJJGJGCJJJ=1JJJJ=JGGCG=GGCGGGCCC NH:i:1 HI:i:1 AS:i:31 nM:i:0 ch:A:1 NM:i:0 SA:Z:22,23289579,-,43M717N75M32S,60,0;
+BCR-ABL1-4 2193 9 130854064 60 107H43M 22 23289525 0 AAGCCCTTCAGCGGCCAGTAGCATCTGACTTTGAGCCTCAGGG CJJJ(JJGJGJJJGGJJGJCC1JJCGGJGG=GGGGGGGGGCCC NH:i:1 HI:i:1 AS:i:42 nM:i:0 ch:A:1 NM:i:0 SA:Z:22,23289590,-,32M717N75M43S,60,0;
+BCR-ABL1-60 147 9 130854074 60 150M = 130854064 -160 GCGGCCAGTAGCATCTGACTTTGAGCCTCAGGGTCTGAGTGAAGCCGCTCGTTGGAACTCCAAGGAAAACCTTCTCGCTGGACCCAGTGAAAATGACCCCAACCTTTTCGTTGCACTGTATGATTTTGTGGCCAGTGGAGATAACACTCT GCGGCCCGCGG=GG8GGCGGGGCCGCC=GCGCGGCG1GGCGG1JCJJ8CCCG=GGGGGGCG=GCGGGG18CCGCG=GGGG1CGG8C=GGGGCGGCJGJGJGJGJJGGJGJJJGJGJJJGGJC(JJJGJJJGJCJJGCGGGGGCGGGGCC= NH:i:1 HI:i:1 AS:i:259 nM:i:0 ch:A:1 NM:i:0
+BCR-ABL1-68 147 9 130854089 60 150M = 130854064 -175 TGACTTTGAGCCTCAGGGTCTAAGTGAAGCCGCTCGTAGGAACTCCAAGGAAAACCTTCTCGGTGGACCCAGTGAAAATGACCCCAACCTTTTCGTTGCACTGTATGATTTTGTGGCCAGTGGAGATAACACTCTAAGCATAACTAAAGG G=CGGCGGGCCGCGCGGCCGG8GCCGCC(GGC=8=GGG=GGGCJ1=JJCCGGGCGGGGGGGG=GCGGGGCGCG==GGCGGGGGJGCCJJGGCCG=GCCCGGJCGGJJJJJ=GJJJJGJCJ=GCJGJGJJJC1GGJJJGGG=GG1GGGCCC NH:i:1 HI:i:1 AS:i:291 nM:i:3 NM:i:3
+BCR-ABL1-50 99 9 130854089 60 150M = 130854133 757 TGACTTTGAGCCTCAGGGTCTGAGTGAAGCCGCTCGTTGGAACTCCAAGGAAAACCTTCTCGCTGGACCCAGTGAAAATGACCCCAACCTTTTCGTTGCACTGTATGATTTTGTGGCCAGTGGAGATAACACTCTAAGCATAACTAAAGG CC1GGCGCGGGG=JGCJJJJJGJJJGJJGGJJGJJ8JGCGJJJJJJ8CJJGJJCGJGGGGJJCCG=CGGGGGCCCG=CGGCCGCGGGGGCGG=GGGGGGGCCGGG==GGGGGCCGG=GGGGCCG=GGGGGCCC=GGCGGGGCCGGCCGGG NH:i:1 HI:i:1 AS:i:300 nM:i:0 XS:A:+ NM:i:0
+BCR-ABL1-28 83 9 130854092 60 146M563N4M = 130854061 -744 CTTTGAGCCTCAAGGTCTGAGTGAAGCCGCTCGTTGGAACTCCAAGGAAAACCTTCTCGCTGGACCCAGTGAAAATGACCCCAACCTTTTCGTTGCACTGTATGATTTTGTGGCCAGTGGAGATAACACTCTAAGCATAACTAAAGGTGA GGGGGCGGGG=C1=8GGCGCGGGCGCGGGGG=GC=GGGGCG1CCCGCGGCCGGGC=GG=GGGGGCCGGGGCGGGCGJJGGGGCGGJ1JGGGGCGGJGJGGJJJCGGCJJCGJ=GJGCGGJJJJGGJJG1JJJGG1JJ=GGCGCGGG1CCC NH:i:1 HI:i:1 AS:i:275 nM:i:3 XS:A:+ NM:i:1
+BCR-ABL1-48 83 9 130854101 60 137M563N13M = 130854061 -753 TCAGGGTCTGAGTGAAGCCGCTCGTTGGAACTCCAAGGAAAACCTTCTCGCTGGACCCAGTGAAAATGACCCCAACCTTTTCGTTGCACTGTATGATTTTGTGGCCAGTGGAGATAACACTCTAAGCATAACTAAAGGTGAAAAGCTCCG GGGCGGCGCGGGG8GGGGGCGGCGGGCG1GCGCGG8GCGGCGC1G8CCGCGCGGCCGGGGGCGCCGCC1=CCCCGCCJCGGGGGGJJGJC=CCJ8JJC=JJCGCJJJGJJJJJJJJJJGJJGGGCJJJJJJJGJGJGCGGCGGGCGC=C1 NH:i:1 HI:i:1 AS:i:295 nM:i:1 XS:A:+ NM:i:0
+BCR-ABL1-76 147 9 130854103 60 135M563N15M = 130854061 -755 AGGGTCTGAGTGAAGCCGCTCGTTGGAACTCCAAGGAAAACCTTCTCGCTGGACCCAGTGAAAATGACCCCAACCTTTTCGTTGCACTGTATGATTTTGTGGCCAGTGGAGATAACACTCTAAGCATAACTAAAGGTGAAAAGCTCCGGG CGGCGGGGCGGGGGGCGCCGCGCGGGGC=GGCGCCGCGCGGGCJJCJC1GGGGG=GCGGGGG=GGGGGGGGGGGGGCGGCGGGGGGJGJCGJGGJGJCJGJJJJJG8JJCJGG1JJJJJJJG8(JJJJJGJJJGJJJGGGGGGGG1GCCC NH:i:1 HI:i:1 AS:i:274 nM:i:1 XS:A:+ NM:i:0
+BCR-ABL1-64 147 9 130854104 60 134M563N16M = 130854061 -756 GGGTCTGAGTGAAGCCGCTCGTTGGAACTCCAAGGAAAACCTTCTCGCTGGACCCAGTGAAAATGACCCCAACCTTTTCGTTGCACTGTATGATTGTGTGGCCAGTGGAGATAACACTCTAAGCATAACTAAAGGTGAAAAGCTCCGGGT C=GGGGGCGGCGGGGGGC=GGGGC1CG=1=GGGCCC=GGGCGCCCCJJGCGCGGGGCGGCGCGCCGCCGCGGGGGCGC1GGGGG=GG1CGJGJJJ(CCGGJJGCGJGJGJJJGGGCGJGJJJJJJJJJGJGJGJJJCGCGGGGGGGGCCC NH:i:1 HI:i:1 AS:i:290 nM:i:2 XS:A:+ NM:i:1
+BCR-ABL1-14 99 9 130854110 60 128M563N22M = 130854134 737 GAGTGAAGCCGCTCGTTGGAACTCCAAGGAAAACCTTCTCGCTGGACCCAGTGAAAATGACCCCAACCTTTTCGTTGCACTGTATGATTTTGTGGCCAGTGGAGATAACACTCTAAGCATAACTAAAGGTGAAAAGCTCCGGGTCTTAGG CC=GGGGGGGGGGJGJJCJGJJJGG1JJGJ=JGGGJJJJGGJJJGCJJCGJJGC=GCJGGJGGCGGGCCGGGCGGCGCGGGGGGGGGGGGCC8GGGGCGCJCGGGCCCGCG8GGGGCGGGCGGCGGGGGGCGGGGGCGGG=GGGGGGCCG NH:i:1 HI:i:1 AS:i:302 nM:i:0 XS:A:+ NM:i:0
+BCR-ABL1-78 99 9 130854115 60 123M563N27M = 130854164 762 AAGCCGCTCGTTGGAACTCCAAGGAAAACCTTCTCGCTGGACCCAGTGAAAATGACCCCAACCTTTTCGTTGCACTGTATGATTTTGTGGCCAGTGGAGATAACACTCTAAGCATAACTAAAGGTGAAAAGCTCCGGGTCTTAGGCTATA CC=1G=GGGGCGGJJJJGJJGG8JJCJGJGJJ8JJJJGJCGJGJJ=JGGGCGJCJCGGG=JJJJGGG=JGC=GGGCGGGGGGCGGCG=GCCGGGGGGCGGJCGCCGCGGGGGGGCCGGGGCGCCGGG=GGGCGCGGGGCGGGCCGGGGGG NH:i:1 HI:i:1 AS:i:302 nM:i:0 XS:A:+ NM:i:0
+BCR-ABL1-62 163 9 130854121 60 117M563N33M = 130854179 771 CTCGTTGGAACTCCAAGGAAAACCTTCTCGCTGGACCCAGTGAAAATGACCCCAACCTTTTCGTTGCACTGTATGATTTTGTGGCCAGTGGAGATAACACTCTAAGCATAACTAAAGGTGAAAAGCTCCGGGTCTTAGGCTATAATCACA CCCCGGGGGGGGCJGGGJJGJJJJJJJ=GJJCJJGJCJGJCGCGCGGGCCJGJCGJ81JC1GGGGGCG8GGCGGGGCG1C1GGGGCGGCGCCCGGG=GC=CGCJJJJGGGGGCGGCGC=8GCCGGGGGGGG=GCG=1GGGCGGGCGG1CG NH:i:1 HI:i:1 AS:i:300 nM:i:1 XS:A:+ NM:i:0
+BCR-ABL1-50 147 9 130854133 60 105M563N45M = 130854089 -757 CCAAGGAAAACCTTCTCGCTGGACCCAGTGAAAATGACCCCAACCTTTTCGTTGCACTGTATGATTTTGTGGCCAGTGGAGATAACACTCTAAGCATAACTAAAGGTGAAAAGCTCCGGGTCTTAGGCTATAATCACAATGGGGAATGGT CCGCCCGGGGGCGGGG1GCGGGGGGC8CGG=CGGCCC=CGGGCCJ(JJ=GGCGCGGGGCGGGGGCC8GCCCGGCGCGGGJ8GGGGCC1JJGJCGGJJJGJG8JJGJJJJCJJJGGJGGGCJGGJJJJGGGJJJ=CJCGG=GCGGGGG=CC NH:i:1 HI:i:1 AS:i:300 nM:i:0 XS:A:+ NM:i:0
+BCR-ABL1-14 147 9 130854134 60 104M563N46M = 130854110 -737 CAAGGAAAACCTTCTCGCTGGACCCAGTGAAAATGACCCCAACCTTTTCGTTGCACTGTATGATTTTGTGGCCAGTGGAGATAACACTCTAAGCATAACTAAAGGTGAAAAGCTCCGGGTCTTAGGCTATAATCACAATGGGGAATGGTG 1CGGGGGCGGCCGG1CG=GGGGGGGCGGGGGCCGGG1CGCGCCJJJJJCG1CGGGCCGCGGGGGGGGGGGGCGGGGGGGCCGGGCGJJGG=JJ(J18GJCJGJ8JJGGJ=JJGJJGGGJJJ=JJJJCJJJJJJJJJGGGGGGG1GGGCCC NH:i:1 HI:i:1 AS:i:302 nM:i:0 XS:A:+ NM:i:0
+BCR-ABL1-20 163 9 130854136 60 102M563N48M = 130854183 760 AGGAAAACCTTCTCGCTGGACCCAGTGAAAATGACCCCAACCTTTTCGTTGCACTGTATGATTTTGTGGCCAGTGGAGATAACACTCTAAGCATAACTAAAGGTGAAAAGCTCCGGGTCTTAGGCTATAATCACAATGGGGAATGGTGTG CC=GGGGGGGGGGJJGJJJJJJCJJ=JJJJGGGGJCJJGCGCGJGGCCCJJJGJJJJCGGJG=GGJGGGGGJGJGCCGGGGCCG=GG=C=G=GGCCGGGCGCCCC=JG11GGCCGCCCGCGCC8CGGGGCC1CGCGGG=GG=CCC1GGCG NH:i:1 HI:i:1 AS:i:302 nM:i:0 XS:A:+ NM:i:0
+BCR-ABL1-26 163 9 130854138 60 100M563N50M = 130854180 755 GAAAACCTTCTCGCTGGACCCAGTGAAAATGACCCCAACCTTTTCGTTGCACTGTATGATTTTGTGGCCAGTGGAGATAACACTCTAAGCATAACTAAAGGTGCAAAGCTCCGGGTCTTAGGCTATAATCACAATGGGGAATGGTGTGAA C1CGGGCGGGGGGJJJ=JJGGCGGGJJGJJGGJGGJJGJCJGJJ=GGCJJGJJGGCGCGCGGG=JGGG8GGGGCGGGGGCGCCCGGGCGGGCCCCCG=GGGCJ(JCJ=GGCCGGGGGGGGGGGGGCCGCGGGCCGGGGGCGCGGGGGGGG NH:i:1 HI:i:1 AS:i:298 nM:i:2 XS:A:+ NM:i:1
+BCR-ABL1-34 99 9 130854147 60 91M563N59M = 130854187 753 CTCGCTGGACCCAGTGAAAATGACCCCAACCTTTTCGTTGCCCTGTATGATTTTGTGGCCAGTGGAGATAACACTCTAAGCATAACTAAAGGTGAAAAGCTCCGGGTCTTAGGCTATAATCACAATGGGGAATGGTGTGAAGCCCAAACC CCCGGGGGGGGGGJJJJJGJGJJGG=JJJJJJGJJJGJGJC1JGGGJGGJJGGGJJGG=GCGJGGJCGGGGGGCG=GGCGG1CGGGG=CGGCCGGGCGGGJ88=CGCG=GGGGGC=GGCGGGGG1GGCCGGGG1GGGCGGGGCCCGCGGC NH:i:1 HI:i:1 AS:i:300 nM:i:1 XS:A:+ NM:i:1
+BCR-ABL1-80 99 9 130854163 60 75M563N75M = 130854214 764 AAAATGACCCCAACCTTTTCGTTGCACTGTATGATTTTGTGGCCAGTGGAGATAACACTCTAAGCATAACTAAAGGTGAAAAGCTCCGGGTCTTAGGCTATAATCACAATGGGGAATGGTGTGAAGCCCAAACCAAAACTGGCCAAGGCT CC=GGGGGGGGGGJJJJCJCJJJJJJGGGJJJCJGCCJJJJJ=JCGJJJ=J8JGJGJ=J=JGG=CJCCGG1GG=CGGG=8GG=GCGCCGGCGCGGGG8GGJG1CGCGCCGCCGCGG=GGGGCGCGGGGGG=G==GGCC(GGCGGGGCGCC NH:i:1 HI:i:1 AS:i:300 nM:i:1 XS:A:+ NM:i:1
+BCR-ABL1-8 163 9 130854163 60 75M563N75M = 130854210 760 AAAATGACCCCAACCTTTTCGTTGCACTGTATGATTTTGTGGCCAGTGGAGATAACACTCTAAGCATAACTAAAGGTGAAAAGCTCCGGGTCTTAGGCTATAATCACAATGGGGAATGGTGTGAAGCCCAAACCAAAAATGGCCAAGGCT =C=GG(1GGGGGGJGGJGCCJJCJJJJJJ=GJGGJJGJ=JJGCGCGJJJJJGGJ1GGJJG8C8GGG=GCCG8GGGG1=CGG88CG=GG8GG=GGGCGG8GGCJ18CJ=CGGCGGGG1CC=GCCGCGGG=GGGCGGCGCC8GCCGCGCCGG NH:i:1 HI:i:1 AS:i:302 nM:i:0 XS:A:+ NM:i:0
+BCR-ABL1-78 147 9 130854164 60 74M563N76M = 130854115 -762 AAATGACCCCAACCTTTTCGTTGCACTGTATGATTTTGTGGCCAGTGGAGATAACACTCTAAGCATAACTAAAGGTGAAAAGCTCCGGGTCTTAGGCTATAATCACAATGGGGAATGGTGTGAAGCCCAAACCAAAAATGGCCAAGGCTG GGGGGCG8GGGGGGGCCGCGG=GCCGCGCGCGGGGGGGC=GCGJ88JJ=CGCGGGG=CGGGGG(GG=G(CCGCGGCJGJGGGCCGGCCJJGGJJJGJJGG1G(JJJGCGJJG=J=GJJJGJJJJJ=CGJJJGJGGJJGGCGGCGGGGCCC NH:i:1 HI:i:1 AS:i:302 nM:i:0 XS:A:+ NM:i:0
+BCR-ABL1-42 163 9 130854168 60 70M563N80M = 130854209 754 GACCCCAACCTTTTCGTTGCACTGTATGATTTTGTGGCCAGTGGAGATAACACTCTAAGCATAACTAAAGGTGAAAAGCTCCGGGTCTTAGGCTATAATCACAATGGGGAATGGTGTGAAGCCCAAACCAAAAATGGCCAAGGCTGGGTC CC=GGGGGGGGGGGGJJJGGJJJ=JJJGGGJJGJGCJJJJ(GJGJJCCJGGGJCGJGGJJJGG=G1C8GCGGGG18GCC=GGGGGCCCGC1GGGGCGGCGG=CCJCCGGG==GGGGGGGG1G(GGG=C=GGGG88=CC=GGCGGGGCGGG NH:i:1 HI:i:1 AS:i:302 nM:i:0 XS:A:+ NM:i:0
+BCR-ABL1-62 83 9 130854179 60 59M563N91M = 130854121 -771 TTTCGTTGCACTGTATGATTTTGTGGCCGGTGGAGATAACACTCTAAGCATAACTAAAGGTGAAAAGCTCCGGGTCTTAGGCTATAATCACAATGGGGAATGGTGTGAAGCCCAAACCAAAAATGGCCAAGGCTGGGTCCCAAGCAACTA GCCC1CCCCCGCGCCC=GGGGGGCCGGC8CGGC1GGC=CGGGGCCCGGC8GCC=GCGGGCGGGGGGGCCGJGGGGGGGGGJGG=GJGCGJGGJ=JJGJGJCG=JJJJGJJJJGJJJGJJGCGJCJ=JJJJJGJGGJJGGGGCGGGGGCCC NH:i:1 HI:i:1 AS:i:300 nM:i:1 XS:A:+ NM:i:1
+BCR-ABL1-26 83 9 130854180 60 58M563N92M = 130854138 -755 TTCGTTGTACTGTATGATTTTGTGGCCAGTGGAGATAACACTCTAAGCATAACTAAAGGTGAAAAGCTCCGGGTCTTAGGCTATAATCACAATGGGGAATGGTGTGAAGCCCAAACCAAAAATGGCCAAGGCTGGGTCCCAAGCAACTAC CGGGGGC(GGGCGGCGCCGGGCGGCGCCGGGCGCGGGGCGGC==GGCCG=1GCCGGCGGCGGGCCCGGG=GGGCGCCCCJJGCCC1GJJCJGGGJJJG8JGG=GJJ=GGJJJJCJGCCGJJJJJJGJJJJJGGJGJJGGGGGGGCGCCCC NH:i:1 HI:i:1 AS:i:298 nM:i:2 XS:A:+ NM:i:1
+BCR-ABL1-44 163 9 130854181 60 57M563N93M = 130854224 756 TCGTTGCACTGTATGATTTTGTGGCCAGTGGAGATAACACTCTAAGCATAACTAAAGGTGAAAAGCTCCGGGTCTTAGGCTATAATCACAATGGGGAATGGTGTGAAGCCCAAACCAAAAATGGCCAAGGCTGGGTCCCAAGCAACTACA C=CGGGGGGCGGGJJJJJGJGJJJJJ1JJJJJJ1JJJGCGGG=JGJGJGGJGGCGGCGJGCJG=JJGGCJCGCCCGGGGCCCGGGC=GG=CGGGGGGGGGGGCJJJJGC=GGGGCGGCGGC=CCGG8GGGCGCGGCCCGGCG=GGCGGGC NH:i:1 HI:i:1 AS:i:302 nM:i:0 XS:A:+ NM:i:0
+BCR-ABL1-40 99 9 130854183 60 55M563N94M1S = 130854802 769 GTTGCACTGTATGATTTTGTGGCCCGTGGAGATAACACTCTAAGCATAACTAAAGGTGAAAAGCTCCGGGTCTTAGGCTATAATCACAATGGGGAATGGTGTGAAGCCCAAACCAAAAATGGCCAAGGCTGGGTCCCAAGCAACTACATG CCCCGGGGGGCGGJJJJGGJCJJJ(GJJ=JJJJJCJJJGGGGJJJGGGJJCJJJGJCJCCJJ(CJ=CGGGJ=GGGCJCGGGGCGGGCGGGGGCGGCGGGGCCGGCGCCGGGGGGCCGCGGGGGGGG=GGGCGG=GGGGGCGGG8G=CGG1 NH:i:1 HI:i:1 AS:i:297 nM:i:1 XS:A:+ NM:i:1
+BCR-ABL1-20 83 9 130854183 60 55M563N95M = 130854136 -760 GTTGCACTGTATGATTTTGTGGCCAGTGGAGATAACACTCTAAGCATAACTAAAGGTGAAAAGCTCCGGGTCTTAGGCTATAATCACAATGGGGAATGGTGTGAAGCCCAAACCAAAAATGGCCAAGGCTGGGTCCCAAGCAACTACATC GCG=GGGCC8GCCGCGGGGGGG=GGCCGGCGGGGCCGGGCGG8CGGCGGJGCGGCGGGGGGCGG=GGG1GCGGGGGGGGGC==JJGG=GGGCCJGGGGGGJJJJJGCCGJJGGJGJCJJCJJJJJGGJJJJGJJJJ1GGGGGGGGGGCCC NH:i:1 HI:i:1 AS:i:302 nM:i:0 XS:A:+ NM:i:0
+BCR-ABL1-34 147 9 130854187 60 51M563N99M = 130854147 -753 CACTGTATGATTTTGTGGCCAGTGGAGATAACACTCTAAGCATAACTAAAGGTGAAAAGCTCCGGGTCTTAGGCTATAATCACAATGGGGAATGGTGTGAAGCCCAAACCAAAAATGGCCAAGGCTGGGTCCCAAGCAACTACATCACGC CGGG8GCC=GGGCGGGGGGCGGGGG=GGGGGGGCGGCGCGGGGJJJJCCCGC1GGGGG8GGCC=GGGGGGGGGGGCCGGCCCG=GCJJJGGGGGG81J8=CGJGGGGJGJJCJGGJGGJGCCJJGJJJJJCJJJJGJGGGCGGGG=GCCC NH:i:1 HI:i:1 AS:i:300 nM:i:1 XS:A:+ NM:i:0
+BCR-ABL1-22 99 9 130854201 60 37M563N113M = 130854828 777 GTGGCCAGTGGAGATAACACTCTAAGCATAACTAAAGGTGAAAAGCTCCGGGTCTTAGGCTATAATCACAATGGGGAATGGTGTGAAGCCCAAACCAAAAATGGCCAAGGCTGGGTCCCAAGCAACTACATCACGCCAGTCAACAGTCTG CC1GGGGGCGGGGGGCJJGCGJCJJJJJJJJGJJJJGJJGGG1JGCJJCJJGJJJJJGGGGJGJJCGJJGCC8CGGCGCGGCGCCG1GGCCCCGC=GG=GJCGGGGCGCG=GGGGGCGGC=CGGGGGGGGGCGGCCCGGG(G8GGGGGGG NH:i:1 HI:i:1 AS:i:300 nM:i:0 XS:A:+ NM:i:0
+BCR-ABL1-42 83 9 130854209 60 29M563N121M = 130854168 -754 TGGAGATAACACTCTAAGCATAACTAAAGGTGAAAAGCTCCGGGTCTTAGGCTATAATCACAATGGGGAATGGTGTGAAGCCCAAACCAAAAATGGCCAAGGCTGGGTCCCAAGCAACTACATCACGCCAGTCAACAGTCTGGAGAAACA GGGCGGGCGCCGGGGGCGGGGGG=CGGGGC1CGCGGGCG=GGCG=GGCCCGGGGCGCGGCGGCCGGGGGGG(GG=GGG=GGGCCGGJJGJJ=GGGGGJJGCGJGGJJJJJJGCJGJGG(GGJJJGJJJJJJJ8GJJJCGGGGCGG1G1CC NH:i:1 HI:i:1 AS:i:302 nM:i:0 XS:A:+ NM:i:0
+BCR-ABL1-8 83 9 130854210 60 28M563N122M = 130854163 -760 GGAGATAACACTCTAAGCATAACTAAAGGTGAAAAGCTCCGGGTCTTAGGCTATAATCACAATGGGGAATGGTGTGAAGCCCAAACCAAAAATGGCCAAGGCTGGGTCCCAAGCAACTACATCACGCCAGTCAACAGTCTGGAGAAACAC G=GGCGGGGG=GGGGGGGCCGCCGCGCGGCCGGCGGGCCGGCGGCGGGGJGG=GGGGGGGC=GCGGGCCCG=JGCG=GGGJGGGJGJGGGJCGCGCGGJGGJJJJJJGJGJGJJJG1JJJJJJJJGJCGGJJJJJJCGGGGGGGGCGCCC NH:i:1 HI:i:1 AS:i:302 nM:i:0 XS:A:+ NM:i:0
+BCR-ABL1-80 147 9 130854214 60 24M563N126M = 130854163 -764 ATAACACTCTAAGCATAACTAAAGGTGAAAAGCTCCGGGTCTTAGGCTATAATCACAATGGGGAATGGTGTGAAGCCCAAACCAAAAATGGCCAAGGCTGGGTCCCAAGCAACTACATCACGCCAGTCAACAGTCTGGAGAAACACTCCT CCCCCGGCC8GCG=CGCGCGGG(GGGGGGGGC=GGG=GG=CGCJJJJJGGGGC==G8CGGG=GCGGC1GGGC(GG=G=GGCGJC8GCGJGJCGCJGJGCGJGJJJGGJJGJGJCJJJJGJGJJJGGJJJJJJGJJJJ8GGGGGGGGGCCC NH:i:1 HI:i:1 AS:i:300 nM:i:1 XS:A:+ NM:i:0
+BCR-ABL1-30 163 9 130854218 60 20M563N130M = 130854831 763 CACTCTAAGCATAACTAAAGGTGAAAAGCACCGGGTCTTAGGCTATAATCACAATGGGGAATGGTGTGAAGCCCAAACCAAAAATGGCCAAGGCTGGGTCCCAAGCAACTACATCACGCCAGTCAACAGTCTGGAGAAACACTCCTGGTA CCCGG1GGGGGGGJGJCJGJGJJJ(JGJJ(JGJGC=GJJJGJJGGJGGJJJJGGJGGCCCCJG8CG=GCJCGGGJ=GCGJ1CGGGGCCGCG8CG=GGCGGC(CJJC=CGGCGG(CGGGGGGCGCCGG=GC1GCGG=G1CGGCCCCG===8 NH:i:1 HI:i:1 AS:i:298 nM:i:1 XS:A:+ NM:i:1
+BCR-ABL1-52 99 9 130854220 60 18M563N132M = 130854841 771 CTCTAAGCATAACTAAAGGTGAAAAGCTCCGGGTCCTAGGCTATAATCACAATGGGGAATGGTGTGAAGCCCAAACCAAAAATGGCCAAGGCTGGGTCCCAAGCAACTACATCACGCCAGTCAACAGTCTGGAGAAACACTCCTGGTACC CCCGGGGGGGGGGGJJGCGJGG8GJJJGJJGJG=G=JJGGCJGGGGGJGGJJGJJGJGGGCJ=GJGGGCGJCGGGGGGG8CGGGGGGC=GGCGGGGCGCGJCGCGCCCCGCG11GGGGGGGGGGGGGGCCCGGGGCCGGCGGGGGCGGCG NH:i:1 HI:i:1 AS:i:298 nM:i:1 XS:A:+ NM:i:1
+BCR-ABL1-44 83 9 130854224 60 14M563N136M = 130854181 -756 AAGCATAACTAAAGGTGAAAAGCTCCGGGTCTTAGGCTATAATCACAATGGGGAATGGTGTGAAGCCCAAACCAAAAATGGCCAAGGCTGGGTCCCAAGCAACTACATCACGCCAGTCAACAGTCTGGAGAAACACTCCTGGTACCATGG CGGGCGCGGGGCGCGGGGGCGGGGGGGGGCGGGGGGGG=G1GGGCGCGGCGCGGGGGGGCGGCGCCGCGGC==GGG=1GGG=CJJGCGJGJJJCGCJJ8GJJGGGJJGGJJJGJJGJJJGJJJJJJCJGJ=GGJJ=JGGGGGCGGCGCCC NH:i:1 HI:i:1 AS:i:302 nM:i:0 XS:A:+ NM:i:0
+BCR-ABL1-40 147 9 130854802 60 150M = 130854183 -769 TGAAAAGCTCCGGGTCTTAGGCTATAATCACAATGGGGAATGGTGTGAAGCCCAAACCAAAAATGGCCAAGGCTGGGTCCCAAGCAACTACATCACGCCAGTCAACAGTCTGGAGAAACACTCCTGGTACCATGGGCCTGTGTCCCGCAA =GCCGGGGCGGGG=GG1G8G=G(GGCGGGGGGGGC1C=GCGG=J8JJ1GGGGGGGGGGGGGGGGGC=1G8CGGCJ8GC1GGCGCCGJG1GGJGGJGJ8JJCJJJGJ8GGJJJ=JGJJG=CGG=JJJJJJJ8JJ=JJGGGGGGGGGCGC=C NH:i:1 HI:i:1 AS:i:297 nM:i:1 XS:A:+ NM:i:0
+BCR-ABL1-16 163 9 130854804 60 150M = 130854852 198 AAAAGCTCCGGGTCTTAGGCTATAATCACAATGGGGAATGGTGTGAAGCCCAAACCAAAAATGGCCAAGGCTGGGTCCCAAGCAACTACATCACGCCAGTCACCAGTCTGGAGAAACACTCCTGGTACCATGGGCCTGTGTCCCGCAATG CC8GGG1CCGGGCCJJGJG(JJJJJ(JGJCJGJJJJJJJJGGJGJGJJCCJJJGJGGGGCGGG=C=JGJJG=C8GGGCGGGGGC(GGCGCGCGGCG(CGG=1(C=JCGGGCCGGGGGCGCGGGCGGCGGGCGGCCGGGGGGGG8GGGCGC NH:i:1 HI:i:1 AS:i:296 nM:i:1 NM:i:1
+BCR-ABL1-6 99 9 130854810 60 150M = 130854867 207 TCCGGGTCTTAGGCTATAATCACAATGGGGAATGGTGTGAAGCCCAAACCAAAAATGGCCAAGGCTGGGTCCCAAGCAACTACATCACGCCAGTCAACAGTCTGGAGAAACACTCCTGGTACCATGGGCCTGTGTCCCGCAATGCCGCTG CCCGGGGGGCGGGGJJGJGJ=JJJJJJJJJJGJGGJJJGGJC=GGGGJ=GGCJJJGCGJGJGJJGJGCGJGGJGJCGCGGGGGGGGGG8GC8GC8G=GGGJ=GCGGGGGCCGCCGGGGGGGG1G1=GCGC81CGGGGGCCCGGGGGCGGG NH:i:1 HI:i:1 AS:i:298 nM:i:0 NM:i:0
+BCR-ABL1-32 99 9 130854814 60 150M = 130854858 194 GGTCTTAGGCTATAATCACAATGGGGAATGGTGTGAAGCCCAAACCAAAAATGGCCAAGGCTGGGTCCCAAGCAACTACATCACGCCAGTCAACAGTCTGGAGAAACACTCCTGGTACCATGGGCCTGTGTCCCGCAATGCCGCTGAGTA CCCGGGGGGGGGGJJGGJJJ=JJGJ8JGJ=JGJG=GGGJGCCJJJC=J8JJ=GCCJC8JC=GGGJGG(GGCJGGGG1GC=GJ=GCCGGCCGGG=GGGGGGJGCGGCGG=CGGCGCG=GGC=CGGGGCCGGGGGGCCCCGCCGGGCCGGGG NH:i:1 HI:i:1 AS:i:298 nM:i:0 NM:i:0
+BCR-ABL1-38 163 9 130854818 60 150M = 130854864 196 TTAGGCTATAATCACAATGGGGAATGGTGTGAAGCCCAAACCAAAAATGGCCAAGGCTGGGTCCCAAGCAACTACATCACGCCAGTCAACAGTCTGGAGAAACACTCCTGGTACCATGGGCCTGTGTCCCGCAATGCCGCTGAGTATCTG CCCGCGGGGGGGG=GGJGJGJJJ1=GGCJJJCGJ=GJJJGGCJGGCGJGGJJCGJCGJGGG=GGGGJCGGGGGGJGGGGGGGGG8C=G=GCGG==CG8GCG=JCJJCCGG8CGGCGGCCGGGCCGCGGCGGG=GCGGCGG=8CGGCGCG= NH:i:1 HI:i:1 AS:i:298 nM:i:0 NM:i:0
+BCR-ABL1-22 147 9 130854828 60 150M = 130854201 -777 ATCACAATGGGGAATGGTGTGAAGCCCAAACCAAAAATGGCCAAGGCTGGGTCCCAAGCAACTACATCACGCCAGTCAACAGTCTGGAGAAACACTCCTGGTACCATGGGCCTGTGTCCCGCAATGCCGCTGAGTATCTGCTGAGCAGCG GGG1C8GGG=C=GGGCGCGG=GG=GCGGGGCGGGCGCGGGCGGJ1CCJCCC8GCC=GGCGGGGGGGCGCGCGGGCCGJGCGJGJGGJGCJGGGGGCGGGJ(JCGGGJJJJJJG=1GGJJJGJJJGGJJJJJJGJ1JJGG=GGGGGCGCCC NH:i:1 HI:i:1 AS:i:300 nM:i:0 XS:A:+ NM:i:0
+BCR-ABL1-30 83 9 130854831 60 150M = 130854218 -763 ACAATGGGGAATGGTGTGAAGCCCAAACCAAAAATGGCCAAGGCTGGGTCCCAAGCAACTACATCACGCCAGTCAACAGTCTGGAGAAACACTCCTGGTACCATGGGCCTGTGTCCCGCAATGCCGCTGAGTATCTGCTGAGCAGCGGGA CG8GC==GCGCCGGGGGGG8=CGGGGGC=CGGCGGGGGCG=GGGC=GCCJGGGGCGGCGCGGGGCGCGGGCCCGJGGG8(8GC8GCGCGCJGGGGGGJGGGJGJJJJJGJJJJCGGJJGJJJ=JJJJGGJ=JJJJGGGGGGGGGGGGCCC NH:i:1 HI:i:1 AS:i:298 nM:i:1 XS:A:+ NM:i:0
+BCR-ABL1-36 99 9 130854836 60 150M = 130854883 197 GGGGAATGGTGTGAAGCCCAAACCAAAAATGGCCAAGGCTGGGTCCCAAGCAACTACATCACGCCAGTCAACAGTCTGGAGAAACACTCCTGGTACCATGGGCCTGTGTCCCGCAATGCCGCTGAGTATCTGCTGAGCAGCGGGATCAAT CCCGGGGGGGGGGJJJGGJJ=JJJJGJJJJJ=GGJJJCJ=JGGGJGGJGJJGGGGCGCG=JGCCGJGCJGCGGGJGGCGCG1GJGC=8GGCCCCGG8CCGJCCGGGCGGGCGGGGGCG1GCCGGCGGGGGCCCGGCCCG(G8GC8=CCGC NH:i:1 HI:i:1 AS:i:298 nM:i:0 NM:i:0
+BCR-ABL1-70 163 9 130854838 60 150M = 130854875 187 GGAATGGTGTGAAGCCCAAACCAAAAATGGCCAAGGCTGGGTCCCAAGCAACTACATCACGCCAGTCAACAGTCTGGAGAAACACTCCTGGTACCATGGGCCTGTGTCCCGCAATGCCGCTGAGTATCTGCTGAGCAGCGGGATCAATGG CCCGGG==GGGGGJJJJJJJJJJJ=GJ8J=GJGJGJGGJCCJJJCGG1=GJGJG18JG=GGGC1GGGJ8GCCC1=CCGCGGG(GC1GCG1GCCGC8GG1GG=JJCJ1GGCGCCGCG(CGGCGGGGGG(GGGGGGCGCCGGGGCGCGCCG= NH:i:1 HI:i:1 AS:i:298 nM:i:0 NM:i:0
+BCR-ABL1-52 147 9 130854841 60 150M = 130854220 -771 ATGGTGTGAAGCCCAAACCAAAAATGGCCAAGGCTGGGTCCCAAGCAACTACATCACGCCAGTCAACAGTCTGGAGAAACACTCCTGGTACCATGGGCCTGTGTCCCGCAATGCCGCTGAGTATCTGCTGAGCAGCGGGATCAATGGCAG CG=GGGGC(CGGGGGGGCCGCGCCGGGGG=GGCC8CGGCCCCCCJ=CJ=GGGGGGGGCG=GGGGCG(GC1GGGC=GJCCCGJ=88GJGGJGJGCJGGJJGGJJJGJJJJGCGJJGGJ(GJGGJCJJJCJJJJJ=(JJGGGGGGGGGCCCC NH:i:1 HI:i:1 AS:i:298 nM:i:1 XS:A:+ NM:i:0
+BCR-ABL1-56 99 9 130854849 60 150M = 130854892 193 AAGCCCAAACCAAAAATGGCCAAGGCTGGGTCCCAAGCAACTACATCACGCCAGTCAACAGTCTGGAGAAACACTCCTGGTACCATGGGCCTGTGTCCCGCAATGCCGCTGAGTATCTGCTGAGCAGCGGGATCAATGGCAGCTTCTTGG =CCGC=GGGGGG1JJJGJGJJJCJJCGJJJJJJJJJJCGJJJJJJGGCCJ1JGCGGCGJ8GG(CJGJGJCGGCGG1CC=CG=GCC=GGGGG=GGCGCCG1CGGGGGGG1GGGGGCGGGCGCGGCGG811G8CCGGGGCGGGGGCCG=CGC NH:i:1 HI:i:1 AS:i:298 nM:i:0 NM:i:0
+BCR-ABL1-16 83 9 130854852 60 150M = 130854804 -198 CCCAAACCAAAAATGGCCAAGGCTGGGTCCCAAGCAACTACATCACGCCAGTCAACAGTCTGGAGAAACACTCCTGGTACCATGGGCCTGTGTCCCGCAATGCCGCTGAGTATCTGCTGAGCAGCGGGATCAATGGCAGCTTCTTGGTGC CGGGGGG=GGCGGGCGGGGGGGCGGGGCGGGGCCCG=CGGGGGCGGGCCCGG8GCCCGG8GCGGC=GCCCGGGGGGCGGGCGGJGGGCCGGJCGGJJGJGGGGGJJJJGJJGGJJGG=JGGJJGGJ=JGJJJJJJGJC1GGGG=GGG1C1 NH:i:1 HI:i:1 AS:i:296 nM:i:1 NM:i:0
+BCR-ABL1-32 147 9 130854858 60 150M = 130854814 -194 CCAAAAATGGCCAAGGCTGGGTCCCAAGCAACTACATCACGCCAGTCAACAGTCTGGAGAAACACTCCTGGTACCATGGGCCTGTGTCCCGCAATGCCGCTGAGTATCTGCTGAGCAGCGGGATCAATGGCAGCTTCTTGGTGCGTGAGA 8GCCCCGGCGCGC1GGGGCGGCCGGGGCG1(GG=GG=GCGGCGJCJJCGG8=GGGCCGCGGGCGGGGGC=GC1=GGGGGJGGCCGJJCGGGJJJCGGG8CCGGGCJGJGJJGCCGCJJJJJJJJJJGCGJJJGJJGJGGGGGCGGGG=CC NH:i:1 HI:i:1 AS:i:298 nM:i:0 NM:i:0
+BCR-ABL1-38 83 9 130854864 60 150M = 130854818 -196 ATGGCCAAGGCTGGGTCCCAAGCAACTACATCACGCCAGTCAACAGTCTGGAGAAACACTCCTGGTACCATGGGCCTGTGTCCCGCAATGCCGCTGAGTATCTGCTGAGCAGCGGGATCAATGGCAGCTTCTTGGTGCGTGAGAGTGAGA GCGCCGCGGGCGGGGGGGCGGCGCG8CGCGGGG8GGCGGGCCCCG8CC=JGCGGGGGGCGGGGCGCCGC=GCCCGGJGGGCGGGCJGCCJJGJG=GGCJJJGGJCGJCGCGJJJC=JJGJCJGJGJJGJJJ=JJJ1GGGGGGGGGGGCCC NH:i:1 HI:i:1 AS:i:298 nM:i:0 NM:i:0
+BCR-ABL1-6 147 9 130854867 60 150M = 130854810 -207 GCCAAGGCTGGGTCCCAAGCAACTACATCACGCCAGTCAACAGTCTGGAGAAACACTCCTGGTACCATGGGCCTGTGTCCCGCAATGCCGCTGAGTATCTGCTGAGCAGCGGGATCAATGGCAGCTTCTTGGTGCGTGAGAGTGAGAGCA CG8GCGGGCCCCGGCGCGGGGCCGGGCGG8CG=G=GCCGCGG=1CJ8JCCGCGGGGGCGGGGGGCGG=G=8GCGJG=GGGGGGJGCCGGJJGG=G=CJ8=JJJJGG=JJJJJGJJGJGJJGJJJJJCCCJJJGGJJJG1GGG1GGGGCCC NH:i:1 HI:i:1 AS:i:298 nM:i:0 NM:i:0
+BCR-ABL1-70 83 9 130854875 60 150M = 130854838 -187 TGGGTCCCAAGCAACTACATCACGCCAGTCAACAGTCTGGAGAAACACTCCTGGTACCATGGGCCTGTGTCCCGCAATGCCGCTGAGTATCTGCTGAGCAGCGGGATCAATGGCAGCTTCTTGGTGCGTGAGAGTGAGAGCAGTCCTGGC CGGGCGGGCGGGCCGGGG=GGG8GCGGG8GGCGGGCGGCGGGCGGGGG=JGGGGGCGCGGGGGCGGGGCGCG1GGGGCJC8GG=JGGJJCCCJJGGGGJGJGJGGJJGGJJJJJGCJJJJGGJJGJJGJJJGGGJJJGGGGCGGGGG=CC NH:i:1 HI:i:1 AS:i:298 nM:i:0 NM:i:0
+BCR-ABL1-36 147 9 130854883 60 150M = 130854836 -197 AAGCAACTACATCACGCCAGTCAACAGTCTGGAGAAACACTCCTGGTACCATGGGCCTGTGTCCCGCAATGCCGCTGAGTATCTGCTGAGCAGCGGGATCAATGGCAGCTTCTTGGTGCGTGAGAGTGAGAGCAGTCCTGGCCAGAGGTC C=GGGGGGCCGGGGGGGGG=GGCCGCGGGC1GCGC1GCGGCCGJJ(CCG8GCCGGGCCGGGC=CC1CG=CGCCGGG=CGGGGGGGGCGGGGGJ==JJJJJ1CJJJGJGGJCGCJGJGJGJJJJ=GG1CJJCGJG1GC=GGGCGCGGGCCC NH:i:1 HI:i:1 AS:i:298 nM:i:0 NM:i:0
+BCR-ABL1-56 147 9 130854892 60 150M = 130854849 -193 CATCACGCCAGTCAACAGTCTGGAGAAACACTCCTGGTACCATGGGCCTGTGTCCCGCAATGCCGCTGAGTATCTGCTGAGCAGCGGGATCAATGGCAGCTTCTTGGTGCGTGAGAGTGAGAGCAGTCCTGGCCAGAGGTCCATCTCGCT CCGGGCGCGCGGGCG=CCCGGCGCGGGGC=CGGCGGCCGCGGGJJJJCCGCCG(GCCCCCGGCCGGG=G8GGGGGGCC=C=CGGJGJJJGC=JGGJJJGJGJ1JJJGC=JJJG=JCJJJJJJJ=JJGGGJJJCGJJJGGGGGCGG=GCCC NH:i:1 HI:i:1 AS:i:298 nM:i:0 NM:i:0
+BCR-ABL1-46 163 22 23285101 60 75M75S = 23285151 5255 AACTGGAGGCAGTGCCCAACATCCCCCTGGTGCCCGATGAGGAGCTGGACGCTTTGAACATCAAGATCTCCAAGAAGTGTTTCAGAAGCTTCTCCCTGACATCCGTGGAGCTGCAGATGCTGACCAACTCGTGTGTGAAACTCCAGACTG CCCGGGGGG=GGGJJJGGJJJGGJJJJCJJGGJJGCJGCGGGC8J8JGGJJJJJGJJC(JGCCG=GGJJGCCCGC8GCCGGGGGG=GGCGGG1GG=GC1G=CJCJJCCCGGCGG1CGG1GGGGGGGG=GGGGGCCGCGGG8GGGCGG=GG NH:i:1 HI:i:1 AS:i:214 nM:i:2 XS:A:+ NM:i:2
+BCR-ABL1-72 163 22 23285110 60 62M2994N7M1344N81M = 23288166 5264 CAGTGCCCAACATCCCCCTGGTGCCCGATGAGGAGCTGCACGCTTTGAAGATCAAGATCTCCAAGAAGTGTTTCAGAAGCTTCTCCCTGACATCCGTGGAGCTGCAGATGCTGACCAACTCGTGTGTGAAACTCCAGACTGTCCACAGCA CCCCGGGGGGGGGGJGJCCCJ1GJJJJGCGGGCJJJ=C1JJGGJGG8JGC=CCGJ1JGG8GGGGGJCGJCCGGGCG=CGGGGGGCGG=GGCGGG=8CCGCGGJJJ=JGGGCGGGGGCCGCCGGGGGGGGC=CCGCG8GGGGGC1GGGGCC NH:i:1 HI:i:1 AS:i:290 nM:i:1 XS:A:+ NM:i:1
+BCR-ABL1-46 83 22 23285151 60 21M2994N7M1344N105M717N17M = 23285101 -5255 GCTTTGAAGATCAAGATCTCCAAGAAGTGTTTCAGAAGCTTCTCCCTGACATCCGTGGAGCTGCAGATGCTGACCAACTCGTGTGTGAAACTCCAGACTGTCCACAGCATTCCGCTGACCATCAATAAGGAAGATGATGAGTCTCCGGGG =GGCGGGGGGG=GGGCCCGCCCGGGGGGGGGGCCGGGGCGG8CGCGGG1JGGCCGG(C=GCCCGGGGGGCGGGGGCGCGGCGGJCGGGJJGJGGGJJCGGGJJJGJJJJJJJGJJJJGGGJJJJJGGJJJJJGCJJJCGGGGGGGGGCCC NH:i:1 HI:i:1 AS:i:214 nM:i:2 XS:A:+ NM:i:0
+BCR-ABL1-72 83 22 23288166 60 3S7M1344N105M717N35M = 23285110 -5264 TCCAAGAAGTGTTTCAGAAGCTTCTCCCTGACATCCGTGGAGCTGCAGATGCTGACCAACTCGTGTGTGAAACTCCAGACTGTCCACAGCATTCCGCTGACCATCAATAAGGAAGATGATGAGTCTCCGGGGCTCTATGGGTTTCTGAAT =GGGGGG==GGGGCCCC=GGGGG=GGGGCGGGCGGGGGGG=CGGCCGCCJGGCGGGGG=GGG8GGGCGGC=G=CCJGGGGGGCGJJGJJCGGGGGGJJJGCJCCGJG=JJJGJGJJCJJJJGJJJJJJJ=GCJGJGCGGG=GGGGGGCC= NH:i:1 HI:i:1 AS:i:290 nM:i:1 XS:A:+ NM:i:0
+BCR-ABL1-4 99 22 23289525 60 97M717N53M = 23289590 889 AGCTTCTCCCTGACATCCGTGGAGCTGCAGATGCTGACCAACTCGTGTGTGAAACTCCAGACTGTCCACAGCATTCCGCTGACCATCAATAAGGAAGATGATGAGTCTCCGGGGCTCTATGGGTTTCTGAATGTCATCGTCCACTCAGCC C==GGGGGGGGGGJJJJ1JJJGGJJGGJGGJJGJJCJGJGJJCGGCJGCJJJJCGJGGGGJGGGGGGCCGG8JGGCGCGG=GGGGGGGGGGGGGG=GCCGJGGGCCGGGGGG1GGGGGGCGCGGCGGGGGG=GGGGGGGGGCCGCGGGCC NH:i:1 HI:i:1 AS:i:259 nM:i:0 ch:A:1 XS:A:+ NM:i:0
+BCR-ABL1-18 99 22 23289532 60 90M717N60M = 23289579 882 CCCTGACATCCGTGGAGCTGCAGATGCTGACCAACTCGTGTGTGAAACTCCAGACTGTCCACAGCATTCCGCTGACCATCAATAAGTAAGATGATGAGTCTCCGGGGCTCTATGGGTTTCTGAATGTCATCGTCCAATCAGCCACTGGAT CCCGGGCGGGCGGJGJJJJJJJJJ=GCJJCJJJJJGJJJGJJGJJJCGGJJGGJCGJC=GG8GCGJGCGCG==GGGCGGGGG1CCCGCGGGGGCGGCCGGC=GCGGG=GGGGCGGGGCGCGGGGGGG=GGGCGGGG(GGGCGGGCGCCGG NH:i:1 HI:i:1 AS:i:266 nM:i:2 ch:A:1 XS:A:+ NM:i:2
+BCR-ABL1-12 163 22 23289546 60 76M717N74M = 23290337 868 GAGCTGCAGATGCTGACCAACTCGTGTGTGAAACTCCAGACTGTCCACAGCATTCCGCTGACCATCAATAAGGAAGATGATGAGTCTCCGGGGCTCTATGGGTTTCTGAATGTCATCGTCCACTCAGCCACTGGATTTAAGCAGAGTTCA CCCGGGGGGGGGGJGGJJJJJCCJJCJJGJJGJJGGCJJJCJCGGGJJ=CGJGJJJJGGCGGGJJJ==GG(GGC=GGGGGGCGCGG(GGGGC1C8GCC=GG=C=CCJGGGGGG8CGGCCCGCGGGGGGGGCGGGG=GGGGGCGGGG=GGC NH:i:1 HI:i:1 AS:i:227 nM:i:0 XS:A:+ NM:i:0
+BCR-ABL1-18 147 22 23289579 60 43M717N75M32S = 23289532 -882 CTCCAGACTGTCCACAGCATTCCGCTGACCATCAATAAGGAAGATGATGAGTCTCCGGGGCTCTATGGGTTTCTGAATGTCATCGTCCACTCAGCCACTGGATTTAAGCAGAGTTCAAAAGCCCTTCAGCGGCCAGTAGCATCTGACTTT =GCGGGGG(GGGGGGGGGGGCC=GGC=GGCGGCCGGGGCGGG8JJJJ=GCGGGGG1GGGCCGGGCCGGGCGGCGGGJGC8GCCGCGGCG=GJCGJJGC8GC1JGG=GJJCJC1JGJGGJJJGJGCJJJ=1JJJJ=JGGCG=GGCGGGCCC NH:i:1 HI:i:1 AS:i:266 nM:i:2 ch:A:1 XS:A:+ NM:i:0 SA:Z:9,130854064,-,118H32M,60,0;
+BCR-ABL1-4 147 22 23289590 60 32M717N75M43S = 23289525 -889 CCACAGCATTCCGCTGACCATCAATAAGGAAGATGATGAGTCTCCGGGGCTCTATGGGTTTCTGAATGTCATCGTCCACTCAGCCACTGGATTTAAGCAGAGTTCAAAAGCCCTTCAGCGGCCAGTAGCATCTGACTTTGAGCCTCAGGG CGGCGGGGGCGGCGCCG=GCGCGGGG8GCG881CGG=C=GCCGJJCJCCCGGG8GGCGG=GGGCCCGGCGGCCCCGGCGGGG=GGCGCJJGCGGJG1JGJJJ8JGJJCJJJ(JJGJGJJJGGJJGJCC1JJCGGJGG=GGGGGGGGGCCC NH:i:1 HI:i:1 AS:i:259 nM:i:0 ch:A:1 XS:A:+ NM:i:0 SA:Z:9,130854064,-,107H43M,60,0;
+BCR-ABL1-12 83 22 23290337 60 19S77M54S = 23289546 -868 CGCTGACCATCAATAAGGAAGATGATGAGTCTCCGGGGCTCTATGGGTTTCTGAATGTCATCGTCCACTCAGCCACTGGATTTAAGCAGAGTTCAAAAGCCCTTCAGCGGCCAGTAGCATCTGACTTTGAGCCTCAGGGTCTGAGTGAAG 8CCCGGCCGGCCGGGGGCGG1CCG=GGCGGGGGC1GGGGCCGCGGGGCCJGG=CGGCGGGGCGCGCGGGCGGCGGJG==GGCGCJGCGGGCJGGGGGGGCJGJGGJJJGJGGGGCJJJGJJJGGJGJJJGJJCCJJGGG1GGGGGGG=CC NH:i:1 HI:i:1 AS:i:227 nM:i:0 XS:A:+ NM:i:0
+BCR-ABL1-60 2145 22 23290375 60 39M111H 9 130854074 0 TCATCGTCCACTCAGCCACTGGATTTAAGCAGAGTTCAA =CCGGCGGGGG=GJJGJGGGCJJCJJGJCGJG(J(JJJG NH:i:1 HI:i:1 AS:i:38 nM:i:0 ch:A:1 NM:i:0 SA:Z:9,130854064,+,39S111M,60,0;
+BCR-ABL1-74 77 * 0 0 * * 0 0 TCATTTTCACTGGGTCCAGCGAGAAGGTTTTCCTTGGAGTTCCAACGAGCGGCTTCACTCAGACCCTGAGGCTCAAAGTCAGATGCTACTGGCCGCTGAAGGGCTTTTGAACTCTGCTTAAATCCAGTGGCTGAGTGGACGATGACATTC CC11GGGGGGGGGGCCJJJGCGJJGJJJJJGGGGGGJJJGGJG==GCJCJ=GGJJGGJJGGCJGG=GGGGGJGGJGC=GC=GGGCGGGCGGGGCCGCGGGJCGC=GGC8CGCGCGGGGGGCGCC1GGCGCC=GCCGCGGC8GCGGGCCCG NH:i:0 HI:i:0 AS:i:155 nM:i:2 uT:A:1
+BCR-ABL1-74 141 * 0 0 * * 0 0 CATTCCGCTGACCATCAATAAGGAAGATGATGAGTCTCCGGGGCTCTATGGGTTTCTGAATGTCATCGTCCACTCAGCCACTGGATTTAGGCAGAGTTCAAAAGCCCTTCAGCGGCCAGTAGCATCTGACTTTGAGCCTCAGGGTCTGAG CCCGGGGGGCGCGJGGJJGGJGJJJGJGGJJGGJGJJ1=JCJJGGGJJJJGGGJGCCJGGJGG=J1JG8JGCGGGJG=GC1CGCCGGCG(GGCGGCGGGGGCJC1CCGC==CCGGGGCGGCGGGCCGGCGCGC8CCCCGGG=GGGC=GGG NH:i:0 HI:i:0 AS:i:155 nM:i:2 uT:A:1
+BCR-ABL1-66 77 * 0 0 * * 0 0 TCCAGCGAGAAGGTTTTCCTTGGAGTTCCAACGAGCGGCTTCACTCAGACCCTGAGGCTCAAAGTCAGATGCTACTGGCCGCTGAAGGGCTTTTGAACTCTGCTTAAATCCAGTGGCTGAGTGGACGATGACATTCAGAAACCCATAGAG CCC=GGGGCGGGGJJJJJGJJJJ=JJJGJJ1GJJGJJJJJGJJJJJGGGGCGJJGGGJJJGGCGGGGJGCGG1JCGGG=GCCGCG=GC=G=GCCGGGGG8JGGGGGGGGGGGG=GGCGGC8GGCCGGGC=GGGGGGGGG=CGG=8GGCCG NH:i:0 HI:i:0 AS:i:159 nM:i:0 uT:A:1
+BCR-ABL1-66 141 * 0 0 * * 0 0 CATTCCGCTGACCATCAATAAGGAAGATGATGAGTCTCCGGGGCTCTATGGGTTTCTGAATGTCATCGTCCACTCAGCCACTGGATTTAAGCAGAGTTCAAAAGCCCTTCAGCGGCCAGTAGCATCTGACTTTGAGCCTCAGGGTCTGAG CCCGGGGGGGGGGGGJ=JGJJJJJJJGGJJCCCJGJJ1JJJGCJGGGGJJJJ=GGGJGJGC(GGGGJGGGJG1=GGGGGGGG=G=C=GG8CC8GGGGGCCCCJCCCJGCG=GGCCGGCGGCGGCG==1GCCGGC1GGGGGCGGGGGGCGG NH:i:0 HI:i:0 AS:i:159 nM:i:0 uT:A:1
+BCR-ABL1-58 77 * 0 0 * * 0 0 ATGATGAGTCTCCGGGGCTCTATGGGTTTCTGAATGTCATCGTCCACTCAGCCACTGGATTTAAGCAGAGTTCAAAAGCCCTTCAGCGGCCAGTAGCATCTGACTTTGAGCCTCAGGGTCTGAGTGAAGCCGCTCGTTGGAACTCCAAGG CCCGGCGGGGGGGGJJJJJGJJGJGJGJGJJJJJJJJJCJGJJJJGCG=8GGGJGJGGCGGJGCGJJJCJGGG=CGCCGGCCGGGCGCGGGCGCG1GGGCCCGGGGCG8GCCC=C8CGCGG=CCCGCCCCGGG=CCGGCGGGCGGGGGCG NH:i:0 HI:i:0 AS:i:185 nM:i:3 uT:A:1
+BCR-ABL1-58 141 * 0 0 * * 0 0 TTGGGGTCATTTTCACTGGGTCCAGCGAGAAGGTTTTCCTTGGAGTTCCAACGAGCGGCTTCACTCAGACCCTGAGGCTCAAAGTCAGATTCTACTGGCCGCTGAAGGGCTTTTGAACTCTGCTTAAATCCAGTGGCTGAGTGGACGATG CCCGGGGGGGGGGJJJJJJGJGJJJGGJ=JJJJJJJJGC=GJJGGJJGJJGG1GCJGGGG=JGGG8C=GCCGC==GGGCGGGGGG=GGG=(G=CCGCCGGGGCJJJJGGGC8GCGCGCG8CGGCCGGGCGCGCGG8CCGG8CGGGGGGGG NH:i:0 HI:i:0 AS:i:185 nM:i:3 uT:A:1
+BCR-ABL1-24 77 * 0 0 * * 0 0 CGCAGACCATCAATAAGGAAGATGATGAGTCTCCGGGGCTCTATGGGTTTCTGAATGTCATCGTCCACTCAGCCACTGGATTTAAGCAGAGTTCAAAAGCCCTTCAGCGGCCAGTAGCATCTGACTTTGAGCCTCAGGGGCTGAGTGAAG CC11GCGGGGGGGJCGJGJJCCJJJJGJJJJGJJGGJJJCJJJG8JJJ1GJ=JGGGGJJJCG=8GGCGCCGGGCCGGGCGGGGCGGGGCCGCGGCCGGG=J1GCCC1(CCGGCGGGCCGCGGGCGGGGC=GGCGCCGCC1GCGGGGGCGG NH:i:0 HI:i:0 AS:i:154 nM:i:3 uT:A:1
+BCR-ABL1-24 141 * 0 0 * * 0 0 TTTCACTGGGTCCAGCGAGAAGGTTTTCCTTGGAGTTCCAACGAGCGGCTTCACTCAGACCCTGAGGCTCAAAGTCAGATGCTACTGGCCGCTGAAGGGCTTTTGAACTCTGCTTAAATCCAGTGGCTGAGTGGACGATGACATTCAGAA C=CCGGGGGGGGCJ1GGJJJJ1JJJJJGJJ=GJJG8GGJ=GJGJJGJJGGGCGJGCGGGCGGG8GG=GJJGCG1GCGGJGCCGGCGGGCCGGGCG8GGGGG8C1==CGGCCCGCGGGGC8GCGGG8GGGCGCCGCCGCGGGCGGGGGGCG NH:i:0 HI:i:0 AS:i:154 nM:i:3 uT:A:1
+BCR-ABL1-10 77 * 0 0 * * 0 0 AGGTTGGGGTCATTTTCACTGGGTCCAGCGAGAAGGTTTTCCTTGGAGTTCCAACGAGCGGCTTCACTCAGACCCTGAGGCTCAAAGTCAGATGCTACTGGCCGCTGAAGGGCTTTTGAACTCTGCTTAAATCCAGTGGCTGAGTGGACG CC=GGGGGGGGGG1GJJJJJCJJJJJJJJJJJGJ=GJJJGCJJJJCJGJGCJGJJJGGJJJGGCCGGJGC=GGJ1C8GGGGGGCGCCGGGGGGCGGGCGCCCG1GGCGCGCGGGCC8GCGCGCGC8CCCGCGCGGGGGCGGGGGCGGCGG NH:i:0 HI:i:0 AS:i:181 nM:i:2 uT:A:1
+BCR-ABL1-10 141 * 0 0 * * 0 0 ATAAGGAAGATGATGAGTCTCCGGGGCTCTATGGGTTTCTGAATGTCATCGTCCACTCAGCCACTGGATTTAAGCAGAGTTCAAAAGCCCTTCAGCGGCCAGTAGCATCTGACTTTGAGCCTCAGGGTCTGAGTGAAGCCGCTCGTTGGA 1CCGGCGGGGGG1GGJJJGCC1JJJJCCG=JGGJJGJJJ=GGGGGJJGGGGGGC1J=CJGCGGGGCGC(CGGGGG=GGGGG(G=CGGCGGGGCCCGC=CCCCJJCC8G1GGGGCGGGGGGCGCGGGGGGGCG=GGCCGCCGCC1G=GGGG NH:i:0 HI:i:0 AS:i:181 nM:i:2 uT:A:1