comparison macros.xml @ 5:226949352e31 draft default tip

planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/cat commit a1c079107b72dc08612fa664897bb9d627624e52-dirty

4:aaeb63501369

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The CAT/BAT workflow is described at: https://github.com/dutilh/CAT    
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5:226949352e31

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Optional arguments:
  -r, --range               cut-off range after alignment [0-49] (default: 10).
  -f, --fraction            fraction of bit-score support for each classification
                            [0-0.99] (default: 0.5).
  -p, --proteins_fasta
                            Path to predicted proteins fasta file. If supplied,
                            CAT will skip the protein prediction step.
  -a, --diamond_alignment
                            Path to DIAMOND alignment table. If supplied, CAT will
                            skip the DIAMOND alignment step and directly classify
                            the sequences. A predicted proteins fasta file should
                            also be supplied with argument [-p / --proteins].


DIAMOND specific optional arguments:
  --sensitive     Run DIAMOND in sensitive mode (default: not enabled).

  --block_size    DIAMOND block-size parameter (default: 2.0). Lower
                  numbers will decrease memory and temporary disk space
                  usage.

  --index_chunks
                  DIAMOND index-chunks parameter (default: 4). Set to 1
                  on high memory machines. The parameter has no effect
                  on temporary disk space usage.

  --top
                  DIAMOND top parameter [0-50] (default: 50). Governs
                  hits within range of best hit that are written to the
                  alignment file. This is not the [-r / --range]
                  parameter!


Setting the DIAMOND --top parameter

You can speed up DIAMOND considerably, and at the same time greatly reduce disk usage, by setting the DIAMOND --top parameter to lower values. This will govern hits within range of the best hit that are written to the alignment file.

You have to be very carefull to 1) not confuse this parameter with the r / --range parameter, which does a similar cut-off but after alignment and 2) be aware that if you want to run CAT or BAT again afterwards with different values of the -r / --range parameter, your options will be limited to the range you have chosen with --top earlier, because all hits that fall outside this range will not be included in the alignment file. Importantly, CAT and BAT currently do not warn you if you choose -r / --range in a second run higher than --top in a previous one, so it's up to you to remember this!

If you have understood all this, or you do not plan to tune -r / --range at all afterwards, you can enjoy a huge speedup with much smaller alignment files! For CAT you can for example set --top 11 and for BAT --top 6.

]]></token>
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The CAT/BAT workflow is described at: https://github.com/dutilh/CAT    
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