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#!/nfs_exports/apps/64-bit/gnu-apps/perl5.8.9/bin/perl -w

use strict;
use Carp;
use Getopt::Long;
use English;
use Pod::Usage;
use Data::Dumper;
use DBI;
use DBD::mysql;

my ( $help, $man, $version, $usage );
my ($in, $out);
my $min_inv = 10000;
my $min_total_sclip = 6;
my $add_inv_back;
my $width = 20;
my $optionOK = GetOptions(
	'i|in|input=s'	=> \$in,
	'o|out|output=s'	=> \$out,
	'min_inv_size=i'	=> \$min_inv,
	'min_total_sclip=i'	=> \$min_total_sclip,
	'add_inv_back!'	=> \$add_inv_back,
	'width=i'		=> \$width,
	'h|help|?'		=> \$help,
	'man'			=> \$man,
	'usage'			=> \$usage,
	'v|version'		=> \$version,
);

pod2usage(2) if($man);
pod2usage( -verbose => 99, -sections => "USAGE|REQUIRED ARGUMENTS|OPTIONS" ) 
	if($help or $usage);
pod2usage( -verbose => 99, -sections => "VERSION") if($version);

croak "Missing input file" if(!$in);
open STDOUT, ">$out" if($out);
open my $IN, "<$in" or croak "can't open $in: $OS_ERROR";

my @SV;
my @discard_SV;
while(my $line = <$IN>) {
	chomp $line;
	my ($chrA, $posA, $ortA, $countA, $chrB, $posB, $ortB, $countB, $type) = split /\t/, $line;
	next if( search_sv(\@SV, $chrA, $posA, $chrB, $posB, $type));
	if(($type eq 'ITX' || $type eq 'INV') && (abs($posB - $posA) < $min_inv && ($countA + $countB) < $min_total_sclip)) {
		print STDERR "$line is removed due to possible circulization\n";
		push @discard_SV, $line;
		next;
	}
	push @SV, [$chrA, $posA, $chrB, $posB, $type, $line];
}

close $IN;

foreach my $sv (@SV) {
	print STDOUT $sv->[5];
#	if($sv->[4] eq 'DEL') {
#		my $tmp = get_gene_info($sv->[0], $sv->[1], $sv->[3]);
#		print STDOUT "\t", $tmp if($tmp);
#	}
#	else {
#		print STDOUT "\t", get_gene_info($sv->[0], $sv->[1], $sv->[1]);
#		print STDOUT "\t", get_gene_info($sv->[2], $sv->[3], $sv->[3]);
#	}
	print STDOUT "\n";
}

if($add_inv_back) {
	foreach my $sv (@discard_SV) {
		my ($chrA, $posA, $ortA, $countA, $chrB, $posB, $ortB, $countB, $type) = split /\t/, $sv;
		next if(search_nearby_sv(\@SV, $chrA, $posA, $posB));
		print STDOUT $sv;
		print "\n";
	}
}
close STDOUT if($out);
exit(0);

sub search_nearby_sv {
	my($r_SV, $chrA, $posA, $posB) = @_;
	foreach my $sv (@{$r_SV}) {
		return 1 if($sv->[0] eq $chrA && abs($sv->[1] - $posA) < 10000);
		return 1 if($sv->[2] eq $chrA && abs($sv->[3] - $posA) < 10000);
		return 1 if($sv->[0] eq $chrA && abs($sv->[1] - $posB) < 10000);
		return 1 if($sv->[2] eq $chrA && abs($sv->[3] - $posB) < 10000);
	}
	return;
}

sub search_sv {
	my ($r_SV, $chrA, $posA, $chrB, $posB, $type) = @_;
	
	for(my $i = 0; $i < scalar @{$r_SV}; $i++) {
		my $sv = $r_SV->[$i];
		if($type eq "INV" && $r_SV->[$i][4] eq "ITX" ) {
			if(( $sv->[0] eq $chrA && abs($sv->[1] - $posA) < 20 &&
	            $sv->[2] eq $chrB && abs($sv->[3] - $posB) < 20 )||
				( $sv->[0] eq $chrB && abs($sv->[1] - $posB) < 20 &&
	            $sv->[2] eq $chrA && abs($sv->[3] - $posA) < 20)) {
				delete $r_SV->[$i];
				return;
			}
		}
		else {	
			return 1 if( $sv->[0] eq $chrA && abs($sv->[1] - $posA) < $width &&
				$sv->[2] eq $chrB && abs($sv->[3] - $posB) < $width && $sv->[4] eq $type);
			return 1 if( $sv->[0] eq $chrB && abs($sv->[1] - $posB) < $width &&
				$sv->[2] eq $chrA && abs($sv->[3] - $posA) < $width && $sv->[4] eq $type);
			return 1 if( $sv->[0] eq $chrA && abs($sv->[1] - $posA) < $width &&
				$sv->[2] eq $chrB && abs($sv->[3] - $posB) < $width && $sv->[4] eq $type && $type eq 'CTX');
			return 1 if( $sv->[0] eq $chrB && abs($sv->[1]- $posB) < $width &&
				$sv->[2] eq $chrA && abs($sv->[3] - $posA)  < $width  && $sv->[4] eq $type && $type eq 'CTX');
		}
	}
	return;
}

=head1 NAME

removeDupSV.pl - a program to remove duplicated SVs for CREST.pl


=head1 VERSION

This documentation refers to removeDupSV.pl version 0.0.1.


=head1 USAGE
	The program need an input file generated by CREST.pl	
		removeDupSV.pl -i tumor.predSV.txt -o tumor.predSV.txt.nodup

=head1 REQUIRED ARGUMENTS

	To run the program, several parameter must specified.
	-i	The input file generated by CREST.pl
	-o	The output file

=head1 OPTIONS

	The options that can be used for the program.
	--min_inv_size		For ITX/INV, the minimum event size, default 10000
	--min_total_sclip	For ITX/INV with size smaller than min_inv_size, the min
						number of soft-clipped reads needed to keep it, default 6
	--(no)add_inv_back	Add ITX/INV back if there is another event nearby, 
						default OFF.
	--width				The max breakpoint distance such that it's considered as 
						same breakpoint, default 20
	-h, --help, -?		 Help information
	--man				 Man page.
	--usage				 Usage information.
	--version			 Software version.


=head1 DESCRIPTION

This is a simple program to remove duplicated SVs, also it remove small INV/ITX 
events since most of those are due to library preparation.


=head1 BUGS AND LIMITATIONS

There are no known bugs in this module, but the method is limitted to bam file 
that has soft-clipping cigar string generated.Please report problems to 
Jianmin Wang  ([e-mail])
Patches are welcome.

=head1 AUTHOR

Jianmin Wang ([e-mail])


=head1 LICENCE AND COPYRIGHT

Copyright (c) 2010 by St. Jude Children's Research Hospital.

This program is free software: you can redistribute it and/or modify
it under the terms of the GNU General Public License as published by
the Free Software Foundation, either version 2 of the License, or
(at your option) any later version. 

This program is distributed in the hope that it will be useful, 
but WITHOUT ANY WARRANTY; without even the implied warranty of 
MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the 
GNU General Public License for more details.

You should have received a copy of the GNU General Public License
along with this program. If not, see <http://www.gnu.org/licenses/>.