# HG changeset patch # User jjohnson # Date 1614171713 0 # Node ID 4635a93ebd9100ee56014a5740348ed48d284e45 # Parent ee774248788f0a47bfbb27059846f6c01b5ac45e "planemo upload commit 692ea558cbbefee93859dc2b005fab5ac4970eb8" diff -r ee774248788f -r 4635a93ebd91 fgbio_fastq_to_bam.xml --- a/fgbio_fastq_to_bam.xml Sun Feb 21 23:40:09 2021 +0000 +++ b/fgbio_fastq_to_bam.xml Wed Feb 24 13:01:53 2021 +0000 @@ -76,7 +76,9 @@ - + + + + + + + + + + + + + + pairs much like the CIGAR string in BAM files. Four kinds of operators are recognized: - T identifies a template read - B identifies a sample barcode read - M identifies a unique molecular index read - S identifies a set of bases that should be skipped or ignored + - T identifies a template read + - B identifies a sample barcode read + - M identifies a unique molecular index read + - S identifies a set of bases that should be skipped or ignored The last pair may be specified using a + sign instead of number to denote “all remaining bases”. This is useful if, e.g., fastqs have been trimmed and contain reads of varying length. For example to convert a paired-end run with an index read and where the first 5 bases of R1 are a UMI and the second five bases are monotemplate you might specify: diff -r ee774248788f -r 4635a93ebd91 macros.xml.bak --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/macros.xml.bak Wed Feb 24 13:01:53 2021 +0000 @@ -0,0 +1,56 @@ + + 1.3.0 + 0 + + + fgbio + + + + (([1-9][0-9]*[TBMS])*([+]|[1-9][0-9]*)[TBMS]) + @READ_STRUCTURE_PATTERN@(\s@READ_STRUCTURE_PATTERN@)* + + ^@READ_STRUCTURES_PATTERN@$ + + + ^[A-Za-z][A-Za-z]$ + + + + + + + + + + + pairs much like the CIGAR string in BAM files. Four kinds of operators are recognized: + + - T identifies a template read + - B identifies a sample barcode read + - M identifies a unique molecular index read + - S identifies a set of bases that should be skipped or ignored + +The last pair may be specified using a + sign instead of number to denote “all remaining bases”. This is useful if, e.g., fastqs have been trimmed and contain reads of varying length. For example to convert a paired-end run with an index read and where the first 5 bases of R1 are a UMI and the second five bases are monotemplate you might specify: + +:: + + --input r1.fq r2.fq i1.fq --read-structures 5M5S+T +T +B + +Alternative if you know your reads are of fixed length you could specify: + +:: + + --input r1.fq r2.fq i1.fq --read-structures 5M5S65T 75T 8B + + +]]> + + + + + +