Mercurial > repos > jjohnson > fgbio_group_reads_by_umi
annotate macros.xml @ 1:568816308859 draft
"planemo upload commit 692ea558cbbefee93859dc2b005fab5ac4970eb8"
author | jjohnson |
---|---|
date | Wed, 24 Feb 2021 13:02:09 +0000 |
parents | 900cd2865768 |
children | 9efbd847e5d1 |
rev | line source |
---|---|
0
900cd2865768
"planemo upload commit 61f6c8e7f32f170ad7e66e46dd74e8c5d361a722"
jjohnson
parents:
diff
changeset
|
1 <macros> |
900cd2865768
"planemo upload commit 61f6c8e7f32f170ad7e66e46dd74e8c5d361a722"
jjohnson
parents:
diff
changeset
|
2 <token name="@TOOL_VERSION@">1.3.0</token> |
900cd2865768
"planemo upload commit 61f6c8e7f32f170ad7e66e46dd74e8c5d361a722"
jjohnson
parents:
diff
changeset
|
3 <token name="@VERSION_SUFFIX@">0</token> |
900cd2865768
"planemo upload commit 61f6c8e7f32f170ad7e66e46dd74e8c5d361a722"
jjohnson
parents:
diff
changeset
|
4 <xml name="requirements"> |
900cd2865768
"planemo upload commit 61f6c8e7f32f170ad7e66e46dd74e8c5d361a722"
jjohnson
parents:
diff
changeset
|
5 <requirements> |
900cd2865768
"planemo upload commit 61f6c8e7f32f170ad7e66e46dd74e8c5d361a722"
jjohnson
parents:
diff
changeset
|
6 <requirement type="package" version="@TOOL_VERSION@">fgbio</requirement> |
900cd2865768
"planemo upload commit 61f6c8e7f32f170ad7e66e46dd74e8c5d361a722"
jjohnson
parents:
diff
changeset
|
7 <yield/> |
900cd2865768
"planemo upload commit 61f6c8e7f32f170ad7e66e46dd74e8c5d361a722"
jjohnson
parents:
diff
changeset
|
8 </requirements> |
900cd2865768
"planemo upload commit 61f6c8e7f32f170ad7e66e46dd74e8c5d361a722"
jjohnson
parents:
diff
changeset
|
9 </xml> |
900cd2865768
"planemo upload commit 61f6c8e7f32f170ad7e66e46dd74e8c5d361a722"
jjohnson
parents:
diff
changeset
|
10 <token name="@READ_STRUCTURE_PATTERN@">(([1-9][0-9]*[TBMS])*([+]|[1-9][0-9]*)[TBMS])</token> |
900cd2865768
"planemo upload commit 61f6c8e7f32f170ad7e66e46dd74e8c5d361a722"
jjohnson
parents:
diff
changeset
|
11 <token name="@READ_STRUCTURES_PATTERN@">@READ_STRUCTURE_PATTERN@(\s@READ_STRUCTURE_PATTERN@)*</token> |
900cd2865768
"planemo upload commit 61f6c8e7f32f170ad7e66e46dd74e8c5d361a722"
jjohnson
parents:
diff
changeset
|
12 <xml name="read_structures_validator"> |
900cd2865768
"planemo upload commit 61f6c8e7f32f170ad7e66e46dd74e8c5d361a722"
jjohnson
parents:
diff
changeset
|
13 <validator type="regex" message="">^@READ_STRUCTURES_PATTERN@$</validator> |
900cd2865768
"planemo upload commit 61f6c8e7f32f170ad7e66e46dd74e8c5d361a722"
jjohnson
parents:
diff
changeset
|
14 </xml> |
900cd2865768
"planemo upload commit 61f6c8e7f32f170ad7e66e46dd74e8c5d361a722"
jjohnson
parents:
diff
changeset
|
15 <xml name="sam_tag_validator"> |
900cd2865768
"planemo upload commit 61f6c8e7f32f170ad7e66e46dd74e8c5d361a722"
jjohnson
parents:
diff
changeset
|
16 <validator type="regex" message="">^[A-Za-z][A-Za-z]$</validator> |
900cd2865768
"planemo upload commit 61f6c8e7f32f170ad7e66e46dd74e8c5d361a722"
jjohnson
parents:
diff
changeset
|
17 </xml> |
900cd2865768
"planemo upload commit 61f6c8e7f32f170ad7e66e46dd74e8c5d361a722"
jjohnson
parents:
diff
changeset
|
18 <xml name="sam_sort_order"> |
900cd2865768
"planemo upload commit 61f6c8e7f32f170ad7e66e46dd74e8c5d361a722"
jjohnson
parents:
diff
changeset
|
19 <param argument="--sort-order" type="select" optional="true" label="Sort BAM by"> |
1
568816308859
"planemo upload commit 692ea558cbbefee93859dc2b005fab5ac4970eb8"
jjohnson
parents:
0
diff
changeset
|
20 <option value="TemplateCoordinate">TemplateCoordinate</option> |
0
900cd2865768
"planemo upload commit 61f6c8e7f32f170ad7e66e46dd74e8c5d361a722"
jjohnson
parents:
diff
changeset
|
21 <option value="Coordinate">Coordinate</option> |
900cd2865768
"planemo upload commit 61f6c8e7f32f170ad7e66e46dd74e8c5d361a722"
jjohnson
parents:
diff
changeset
|
22 <option value="Queryname">Queryname</option> |
900cd2865768
"planemo upload commit 61f6c8e7f32f170ad7e66e46dd74e8c5d361a722"
jjohnson
parents:
diff
changeset
|
23 <option value="Random">Random</option> |
900cd2865768
"planemo upload commit 61f6c8e7f32f170ad7e66e46dd74e8c5d361a722"
jjohnson
parents:
diff
changeset
|
24 <option value="RandomQuery">RandomQuery</option> |
900cd2865768
"planemo upload commit 61f6c8e7f32f170ad7e66e46dd74e8c5d361a722"
jjohnson
parents:
diff
changeset
|
25 </param> |
900cd2865768
"planemo upload commit 61f6c8e7f32f170ad7e66e46dd74e8c5d361a722"
jjohnson
parents:
diff
changeset
|
26 </xml> |
1
568816308859
"planemo upload commit 692ea558cbbefee93859dc2b005fab5ac4970eb8"
jjohnson
parents:
0
diff
changeset
|
27 |
568816308859
"planemo upload commit 692ea558cbbefee93859dc2b005fab5ac4970eb8"
jjohnson
parents:
0
diff
changeset
|
28 <xml name="sort_order_change_format"> |
568816308859
"planemo upload commit 692ea558cbbefee93859dc2b005fab5ac4970eb8"
jjohnson
parents:
0
diff
changeset
|
29 <change_format> |
568816308859
"planemo upload commit 692ea558cbbefee93859dc2b005fab5ac4970eb8"
jjohnson
parents:
0
diff
changeset
|
30 <when input="sort_order" value="Coordinate" format="bam" /> |
568816308859
"planemo upload commit 692ea558cbbefee93859dc2b005fab5ac4970eb8"
jjohnson
parents:
0
diff
changeset
|
31 <when input="sort_order" value="TemplateCoordinate" format="bam" /> |
568816308859
"planemo upload commit 692ea558cbbefee93859dc2b005fab5ac4970eb8"
jjohnson
parents:
0
diff
changeset
|
32 <when input="sort_order" value="QueryName" format="unsorted.bam" /> |
568816308859
"planemo upload commit 692ea558cbbefee93859dc2b005fab5ac4970eb8"
jjohnson
parents:
0
diff
changeset
|
33 <when input="sort_order" value="Random" format="unsorted.bam" /> |
568816308859
"planemo upload commit 692ea558cbbefee93859dc2b005fab5ac4970eb8"
jjohnson
parents:
0
diff
changeset
|
34 <when input="sort_order" value="RandomQuery" format="unsorted.bam" /> |
568816308859
"planemo upload commit 692ea558cbbefee93859dc2b005fab5ac4970eb8"
jjohnson
parents:
0
diff
changeset
|
35 </change_format> |
568816308859
"planemo upload commit 692ea558cbbefee93859dc2b005fab5ac4970eb8"
jjohnson
parents:
0
diff
changeset
|
36 </xml> |
0
900cd2865768
"planemo upload commit 61f6c8e7f32f170ad7e66e46dd74e8c5d361a722"
jjohnson
parents:
diff
changeset
|
37 |
900cd2865768
"planemo upload commit 61f6c8e7f32f170ad7e66e46dd74e8c5d361a722"
jjohnson
parents:
diff
changeset
|
38 <token name="@READ_STRUCTURES_HELP@"><![CDATA[ |
900cd2865768
"planemo upload commit 61f6c8e7f32f170ad7e66e46dd74e8c5d361a722"
jjohnson
parents:
diff
changeset
|
39 **Read Structures** |
900cd2865768
"planemo upload commit 61f6c8e7f32f170ad7e66e46dd74e8c5d361a722"
jjohnson
parents:
diff
changeset
|
40 |
900cd2865768
"planemo upload commit 61f6c8e7f32f170ad7e66e46dd74e8c5d361a722"
jjohnson
parents:
diff
changeset
|
41 Read structures are made up of <number><operator> pairs much like the CIGAR string in BAM files. Four kinds of operators are recognized: |
900cd2865768
"planemo upload commit 61f6c8e7f32f170ad7e66e46dd74e8c5d361a722"
jjohnson
parents:
diff
changeset
|
42 |
1
568816308859
"planemo upload commit 692ea558cbbefee93859dc2b005fab5ac4970eb8"
jjohnson
parents:
0
diff
changeset
|
43 - T identifies a template read |
568816308859
"planemo upload commit 692ea558cbbefee93859dc2b005fab5ac4970eb8"
jjohnson
parents:
0
diff
changeset
|
44 - B identifies a sample barcode read |
568816308859
"planemo upload commit 692ea558cbbefee93859dc2b005fab5ac4970eb8"
jjohnson
parents:
0
diff
changeset
|
45 - M identifies a unique molecular index read |
568816308859
"planemo upload commit 692ea558cbbefee93859dc2b005fab5ac4970eb8"
jjohnson
parents:
0
diff
changeset
|
46 - S identifies a set of bases that should be skipped or ignored |
0
900cd2865768
"planemo upload commit 61f6c8e7f32f170ad7e66e46dd74e8c5d361a722"
jjohnson
parents:
diff
changeset
|
47 |
900cd2865768
"planemo upload commit 61f6c8e7f32f170ad7e66e46dd74e8c5d361a722"
jjohnson
parents:
diff
changeset
|
48 The last <number><operator> pair may be specified using a + sign instead of number to denote “all remaining bases”. This is useful if, e.g., fastqs have been trimmed and contain reads of varying length. For example to convert a paired-end run with an index read and where the first 5 bases of R1 are a UMI and the second five bases are monotemplate you might specify: |
900cd2865768
"planemo upload commit 61f6c8e7f32f170ad7e66e46dd74e8c5d361a722"
jjohnson
parents:
diff
changeset
|
49 |
900cd2865768
"planemo upload commit 61f6c8e7f32f170ad7e66e46dd74e8c5d361a722"
jjohnson
parents:
diff
changeset
|
50 :: |
900cd2865768
"planemo upload commit 61f6c8e7f32f170ad7e66e46dd74e8c5d361a722"
jjohnson
parents:
diff
changeset
|
51 |
900cd2865768
"planemo upload commit 61f6c8e7f32f170ad7e66e46dd74e8c5d361a722"
jjohnson
parents:
diff
changeset
|
52 --input r1.fq r2.fq i1.fq --read-structures 5M5S+T +T +B |
900cd2865768
"planemo upload commit 61f6c8e7f32f170ad7e66e46dd74e8c5d361a722"
jjohnson
parents:
diff
changeset
|
53 |
900cd2865768
"planemo upload commit 61f6c8e7f32f170ad7e66e46dd74e8c5d361a722"
jjohnson
parents:
diff
changeset
|
54 Alternative if you know your reads are of fixed length you could specify: |
900cd2865768
"planemo upload commit 61f6c8e7f32f170ad7e66e46dd74e8c5d361a722"
jjohnson
parents:
diff
changeset
|
55 |
900cd2865768
"planemo upload commit 61f6c8e7f32f170ad7e66e46dd74e8c5d361a722"
jjohnson
parents:
diff
changeset
|
56 :: |
900cd2865768
"planemo upload commit 61f6c8e7f32f170ad7e66e46dd74e8c5d361a722"
jjohnson
parents:
diff
changeset
|
57 |
900cd2865768
"planemo upload commit 61f6c8e7f32f170ad7e66e46dd74e8c5d361a722"
jjohnson
parents:
diff
changeset
|
58 --input r1.fq r2.fq i1.fq --read-structures 5M5S65T 75T 8B |
900cd2865768
"planemo upload commit 61f6c8e7f32f170ad7e66e46dd74e8c5d361a722"
jjohnson
parents:
diff
changeset
|
59 |
900cd2865768
"planemo upload commit 61f6c8e7f32f170ad7e66e46dd74e8c5d361a722"
jjohnson
parents:
diff
changeset
|
60 |
900cd2865768
"planemo upload commit 61f6c8e7f32f170ad7e66e46dd74e8c5d361a722"
jjohnson
parents:
diff
changeset
|
61 ]]></token> |
900cd2865768
"planemo upload commit 61f6c8e7f32f170ad7e66e46dd74e8c5d361a722"
jjohnson
parents:
diff
changeset
|
62 <xml name="citations"> |
900cd2865768
"planemo upload commit 61f6c8e7f32f170ad7e66e46dd74e8c5d361a722"
jjohnson
parents:
diff
changeset
|
63 <citations> |
900cd2865768
"planemo upload commit 61f6c8e7f32f170ad7e66e46dd74e8c5d361a722"
jjohnson
parents:
diff
changeset
|
64 <yield /> |
900cd2865768
"planemo upload commit 61f6c8e7f32f170ad7e66e46dd74e8c5d361a722"
jjohnson
parents:
diff
changeset
|
65 </citations> |
900cd2865768
"planemo upload commit 61f6c8e7f32f170ad7e66e46dd74e8c5d361a722"
jjohnson
parents:
diff
changeset
|
66 </xml> |
900cd2865768
"planemo upload commit 61f6c8e7f32f170ad7e66e46dd74e8c5d361a722"
jjohnson
parents:
diff
changeset
|
67 </macros> |