Mercurial > repos > jjohnson > fgbio_group_reads_by_umi
annotate macros.xml @ 0:900cd2865768 draft
"planemo upload commit 61f6c8e7f32f170ad7e66e46dd74e8c5d361a722"
author | jjohnson |
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date | Sun, 21 Feb 2021 23:40:34 +0000 |
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children | 568816308859 |
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1 <macros> |
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2 <token name="@TOOL_VERSION@">1.3.0</token> |
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3 <token name="@VERSION_SUFFIX@">0</token> |
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4 <xml name="requirements"> |
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5 <requirements> |
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6 <requirement type="package" version="@TOOL_VERSION@">fgbio</requirement> |
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7 <yield/> |
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8 </requirements> |
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9 </xml> |
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10 <token name="@READ_STRUCTURE_PATTERN@">(([1-9][0-9]*[TBMS])*([+]|[1-9][0-9]*)[TBMS])</token> |
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11 <token name="@READ_STRUCTURES_PATTERN@">@READ_STRUCTURE_PATTERN@(\s@READ_STRUCTURE_PATTERN@)*</token> |
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12 <xml name="read_structures_validator"> |
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13 <validator type="regex" message="">^@READ_STRUCTURES_PATTERN@$</validator> |
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14 </xml> |
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15 <xml name="sam_tag_validator"> |
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16 <validator type="regex" message="">^[A-Za-z][A-Za-z]$</validator> |
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17 </xml> |
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18 <xml name="sam_sort_order"> |
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19 <param argument="--sort-order" type="select" optional="true" label="Sort BAM by"> |
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20 <option value="Coordinate">Coordinate</option> |
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21 <option value="Queryname">Queryname</option> |
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22 <option value="Random">Random</option> |
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23 <option value="RandomQuery">RandomQuery</option> |
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24 </param> |
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25 </xml> |
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26 |
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27 <token name="@READ_STRUCTURES_HELP@"><![CDATA[ |
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28 **Read Structures** |
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29 |
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30 Read structures are made up of <number><operator> pairs much like the CIGAR string in BAM files. Four kinds of operators are recognized: |
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31 |
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32 T identifies a template read |
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33 B identifies a sample barcode read |
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34 M identifies a unique molecular index read |
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35 S identifies a set of bases that should be skipped or ignored |
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36 |
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37 The last <number><operator> pair may be specified using a + sign instead of number to denote “all remaining bases”. This is useful if, e.g., fastqs have been trimmed and contain reads of varying length. For example to convert a paired-end run with an index read and where the first 5 bases of R1 are a UMI and the second five bases are monotemplate you might specify: |
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38 |
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39 :: |
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40 |
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41 --input r1.fq r2.fq i1.fq --read-structures 5M5S+T +T +B |
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42 |
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43 Alternative if you know your reads are of fixed length you could specify: |
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44 |
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45 :: |
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46 |
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47 --input r1.fq r2.fq i1.fq --read-structures 5M5S65T 75T 8B |
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48 |
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49 |
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50 ]]></token> |
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51 <xml name="citations"> |
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52 <citations> |
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53 <yield /> |
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54 </citations> |
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55 </xml> |
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56 </macros> |