Mercurial > repos > jjohnson > mothur_toolsuite
changeset 36:040410b8167e default tip
Fixed reference to legacy blast repository in tool_dependencies.xml. Updated README with author information.
author | galaxyuser <galaxy_user@agr.g.ca> |
---|---|
date | Mon, 10 Nov 2014 15:40:02 -0500 |
parents | 95d75b35e4d2 |
children | |
files | mothur/README mothur/tool_data_table_conf.xml.sample mothur/tool_dependencies.xml mothur/tools/mothur/pcr.seqs.xml |
diffstat | 4 files changed, 92 insertions(+), 134 deletions(-) [+] |
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--- a/mothur/README Fri Oct 31 15:09:32 2014 -0400 +++ b/mothur/README Mon Nov 10 15:40:02 2014 -0500 @@ -1,20 +1,15 @@ -Provides galaxy tools for the Mothur metagenomics package - http://www.mothur.org/wiki/Main_Page - +Galaxy wrappers for the Mothur metagenomics tools (http://www.mothur.org/wiki/Main_Page) -Mothur should be able to be auto-installed as a tool_dependency - You may want to reorganize the tool panel after installing - See below: Reorganize integrated_tool_panel.xml - This was based on: http://www.mothur.org/wiki/Mothur_manual - (The environment variable MOTHUR_MAX_PROCESSORS can be used to limit the number of cpu processors used for mothur commands) - This will be set in: tool_dependencies/mothur/1.27/jjohnson/mothur_toolsuite/*/env.sh - Requirements for auto installation: - - make (sudo-apt get install make) - - g++ (sudo apt-get install g++) - - gfortran (sudo apt-get install gfortran) - - pip (sudo apt-get install python-pip) +The Mothur Tool Suite repository: + - Provides Mothur wrappers for most Mothur tools + - Data type used by mothur and other metagenomics tools + - Downloads and builds Mothur on the Linux or Mac operating system + +Requirements: + - Build utilities (make, GCC, gfortran, etc) - simplejson (pip install simplejson) - Repository Dependency: +Repository Dependency: - BLAST Legacy ver. 2.2.26 (ftp://ftp.ncbi.nlm.nih.gov/blast/executables/release/LATEST/) - The repository name should be package_blast_2_2_26 so it matches with the tool dependency. @@ -114,29 +109,23 @@ /project/db/galaxy/mothur/RDP/trainset7_112011.rdp.fasta /project/db/galaxy/mothur/RDP/trainset7_112011.rdp.tax - - Add tool-data: (contains pointers to silva, greengenes, and RDP reference data) tool-data/mothur_aligndb.loc tool-data/mothur_map.loc tool-data/mothur_taxonomy.loc tool-data/shared/jars/TreeVector.jar - ################################################################ #### If you are manually adding this to your local galaxy: #### ################################################################ add config files (*.xml) and wrapper code (*.py) from tools/mothur/* to your galaxy installation - add datatype definition file: lib/galaxy/datatypes/metagenomics.py add the following import line to: lib/galaxy/datatypes/registry.py import metagenomics # added for metagenomics mothur - - add datatypes to: datatypes_conf.xml add mothur tools to: tool_conf.xml @@ -172,7 +161,3 @@ # Each item conatins: a regex pattern for matching filenames and a galaxy datatype (separated by :) # The regex match.groups()[0] is used as the id name of the dataset, and must result in unique name for each output --new_datasets='^\S+?\.((\S+)\.(unique|[0-9.]*)\.dist)$:lower.dist' - - ## - ## NOTE: The "read" commands were eliminated with Mothur version 1.18 - ##
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/mothur/tool_data_table_conf.xml.sample Mon Nov 10 15:40:02 2014 -0500 @@ -0,0 +1,18 @@ +<tables> + <table name="mothur_aligndb" comment_char="#"> + <columns>name, value</columns> + <file path="tool-data/mothur_aligndb.loc" /> + </table> + <table name="mothur_lookup" comment_char="#"> + <columns>name, value</columns> + <file path="tool-data/mothur_lookup.loc" /> + </table> + <table name="mothur_map" comment_char="#"> + <columns>name, value</columns> + <file path="tool-data/mothur_map.loc" /> + </table> + <table name="mothur_taxonomy" comment_char="#"> + <columns>name, value</columns> + <file path="tool-data/mothur_taxonomy.loc" /> + </table> +</tables> \ No newline at end of file
--- a/mothur/tool_dependencies.xml Fri Oct 31 15:09:32 2014 -0400 +++ b/mothur/tool_dependencies.xml Mon Nov 10 15:40:02 2014 -0500 @@ -1,122 +1,77 @@ -<?eml version="1.0"?> +<?xml version="1.0"?> <tool_dependency> - <!-- - <package name="mothur" version="1.27"> + <package name="mothur" version="1.33"> <install version="1.0"> <actions> - <action type="download_by_url">http://www.mothur.org/w/images/c/cb/Mothur.1.27.0.zip</action> + <action type="download_by_url">http://www.mothur.org/w/images/6/65/Mothur.1.33.3.zip</action> <action type="shell_command">chmod u+x Mothur.source/uchime_src/mk</action> - <action type="shell_command"> - test $(uname) = Linux && - perl -i.bak -pe 's/(USEREADLINE.+) = yes/$1 = no/; s/TARGET_ARCH \+= -arch x86_64//; s/#(CXXFLAGS.+-mtune=native -march=native -m64)/$1/;' Mothur.source/makefile || - true - </action> - <action type="shell_command">cd Mothur.source; make -j 2;</action> - <action type="move_file"> - <source>Mothur.source/mothur</source> - <destination>$INSTALL_DIR/bin</destination> - </action> - <action type="move_file"> - <source>Mothur.source/uchime</source> - <destination>$INSTALL_DIR/bin</destination> - </action> - <action type="set_environment"> - <environment_variable name="PATH" action="prepend_to">$INSTALL_DIR/bin</environment_variable> - <environment_variable name="MOTHUR_MAX_PROCESSORS" action="set_to">2</environment_variable> - </action> - </actions> - </install> - <readme> + <action type="shell_command"> + test $(uname) = Linux && + perl -i.bak -pe 's/(USEREADLINE.+) = yes/$1 = no/; s/TARGET_ARCH \+= -arch x86_64//; s/#(CXXFLAGS.+-mtune=native -march=native -m64)/$1/;' Mothur.source/makefile || + true + </action> + <action type="shell_command">cd Mothur.source; make -j 2;</action> + <action type="move_file"> + <source>Mothur.source/mothur</source> + <destination>$INSTALL_DIR/bin</destination> + </action> + <action type="move_file"> + <source>Mothur.source/uchime</source> + <destination>$INSTALL_DIR/bin</destination> + </action> + <action type="set_environment"> + <environment_variable name="PATH" action="prepend_to">$INSTALL_DIR/bin</environment_variable> + <environment_variable name="MOTHUR_MAX_PROCESSORS" action="set_to">2</environment_variable> + </action> + <action type="set_environment_for_install"> + <repository changeset_revision="8a5a4d8db315" name="package_blast_2_2_26" owner="iyad" toolshed="https://toolshed.g2.bx.psu.edu/"> + <package name="blast" version="2.2.26" /> + </repository> + </action> - Iyad Kandalaft added shell commands to build in Linux environment such as Redhat/Centos/Ubuntu. ReadLine libraries are not required. libncurses-devel are required. - - These links provide information for building the Mothur package in most environments . + <action type="make_directory">$INSTALL_DIR/bin/blast</action> + <action type="make_directory">$INSTALL_DIR/bin/blast/bin</action> + <action type="change_directory">$INSTALL_DIR/bin</action> - http://www.mothur.org/wiki/Download_mothur - - http://www.mothur.org/wiki/Installation - - http://www.mothur.org/wiki/Makefile_options + <action type="shell_command">cd $INSTALL_DIR/bin;mypath=`which blastall`; mypath=`dirname $mypath`; cp $mypath/* ./blast/bin</action> - </readme> - </package> ---> - <package name="mothur" version="1.33"> - <install version="1.0"> - <actions> - <action type="download_by_url">http://www.mothur.org/w/images/6/65/Mothur.1.33.3.zip</action> - <action type="shell_command">chmod u+x Mothur.source/uchime_src/mk</action> - <!-- - <action type="shell_command"> - test -e /etc/redhat-release && - perl -i.bak -pe 's/#(CXX.+g\+\+44)/$1/;' Mothur.source/makefile || - true - </action> - --> - <action type="shell_command"> - test $(uname) = Linux && - perl -i.bak -pe 's/(USEREADLINE.+) = yes/$1 = no/; s/TARGET_ARCH \+= -arch x86_64//; s/#(CXXFLAGS.+-mtune=native -march=native -m64)/$1/;' Mothur.source/makefile || - true - </action> - <action type="shell_command">cd Mothur.source; make -j 2;</action> - <action type="move_file"> - <source>Mothur.source/mothur</source> - <destination>$INSTALL_DIR/bin</destination> - </action> - <action type="move_file"> - <source>Mothur.source/uchime</source> - <destination>$INSTALL_DIR/bin</destination> - </action> - <action type="set_environment"> - <environment_variable name="PATH" action="prepend_to">$INSTALL_DIR/bin</environment_variable> - <environment_variable name="MOTHUR_MAX_PROCESSORS" action="set_to">2</environment_variable> - </action> - <action type="set_environment_for_install"> - <repository changeset_revision="32c158152e5e" name="package_blast_plus_2_2_26" owner="meowmeow" toolshed="http://onotta669816x:9001/"> - <package name="blast" version="2.2.26" /> - </repository> - </action> + </actions> + </install> + <readme> +*Authors & Contacts* +Jim Johnson +Peter Briggs +Iyad Kandalaft | Iyad.Kandalaft@Agr.gc.ca | Agriculture and Agri-Foods Canada +Kevin Chen | Agriculture and Agri-Foods Canada + +*Description* + +The Mothur Tool Suite repository provides Galaxy wrappers for the majority of the Mothur tools. In addition, it downloads and builds the Mothur binaries. + +Supports Linux environments such as Redhat/Centos/Ubuntu as well as Mac OSX. + +These links provide information for building the Mothur package in most environments. + +http://www.mothur.org/wiki/Download_mothur - <!-- - <action type="shell_command">mkdir $INSTALL_DIR/bin/blast; mkdir $INSTALL_DIR/bin/blast/bin;</action> - --> - <action type="make_directory">$INSTALL_DIR/bin/blast</action> - <action type="make_directory">$INSTALL_DIR/bin/blast/bin</action> - <action type="change_directory">$INSTALL_DIR/bin</action> - <!-- - <action type="shell_command">cd $INSTALL_DIR/bin; cp ../../../../../../blast/*/*/package_blast_plus_2_2_26/*/* ./blast/bin</action> - --> +http://www.mothur.org/wiki/Installation +http://www.mothur.org/wiki/Makefile_options - <action type="shell_command">cd $INSTALL_DIR/bin;mypath=`which blastall`; mypath=`dirname $mypath`; cp $mypath/* ./blast/bin</action> - - </actions> - </install> - <readme> - - Iyad Kandalaft added shell commands to build in Linux environment such as Redhat/Centos/Ubuntu. ReadLine libraries are not required. libncurses-devel are required. - - These links provide information for building the Mothur package in most environments . +*Requirements* - http://www.mothur.org/wiki/Download_mothur - - http://www.mothur.org/wiki/Installation - - http://www.mothur.org/wiki/Makefile_options - - </readme> - </package> - <!-- - http://supfam.cs.bris.ac.uk/TreeVector/treevectorsource.zip - --> - - <package name="blast" version="2.2.26"> - <repository - name="package_blast_plus_2_2_26" - owner="meowmeow" - changeset_revision="32c158152e5e" - toolshed="http://onotta669816x:9001/" - prior_installation_required="True"/> - </package> +- build tools (make, C libraries, GCC) +- libncurses-dev(el) +- simplejson (python library) + </readme> + </package> + <package name="blast" version="2.2.26"> + <repository + name="package_blast_2_2_26" + owner="iyad" + changeset_revision="8a5a4d8db315" + toolshed="https://toolshed.g2.bx.psu.edu/" + prior_installation_required="True"/> + </package> </tool_dependency>
--- a/mothur/tools/mothur/pcr.seqs.xml Fri Oct 31 15:09:32 2014 -0400 +++ b/mothur/tools/mothur/pcr.seqs.xml Mon Nov 10 15:40:02 2014 -0500 @@ -40,7 +40,7 @@ --result=#echo ','.join($results) --processors=8 #if $pdiffs.__str__ != '' and int($pdiffs.__str__) > 0: - --pdiffs=$pdiffs + --$pdiffs=$pdiffs #end if </command> <inputs>