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changeset 0:d328db400280 draft default tip

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author jjohnson
date Wed, 05 Feb 2014 09:27:54 -0500
parents
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files test-data/._translated_bed_sequences.fa test-data/Extract_Genomic_DNA.bed test-data/translated_bed_sequences.fa tool_dependencies.xml translate_bed_sequences.py translate_bed_sequences.xml
diffstat 6 files changed, 396 insertions(+), 0 deletions(-) [+]
line wrap: on
line diff
Binary file test-data/._translated_bed_sequences.fa has changed
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/Extract_Genomic_DNA.bed	Wed Feb 05 09:27:54 2014 -0500
@@ -0,0 +1,7 @@
+15	40902459	40907576	JUNC00019210	1	+	40902459	40907576	255,0,0	2	36,38	0,5079	CTCATATGAAAATAGTGAGAAAGTCAGAAATGGAAGGTAAGTATGTTACTATAATTCCTGCTAAATCTGCTTTTTAAAAAATTTTACTGTAGCTATCTAACCAGACAACTAATATAACTTATTGTCTATCTTATTGCTTAAAAAATATATGATGGGCCATGCATAGTGGTTCACACCTGTGATCCCGACACTTTGGCAGTCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCAAGACCAACCTGGATAGCATAGTGAGATTCTGTCTCTACAAAAAATAAAAAATTAGCCAGGTGTGGGGGTGCATTCCTGTAGCCCCAGCTACTCAGGAGGCTGAGGTGAGAGGATCACTTGAGTCCAGAAGATTGAGACTGCAGTGATTGCACCAGTGCACTCCAGCCTGGGCAACAGAGCAAGACTCTGTCTCAAAAACAAAAAAAGTATACCTTGAGTCTTGGGAGTAGAGAGTAGAGTGGATAGTAAGTAAATTTATTTATTTATTTATTTTGAGACGGATTTTCACTGTTGTTCCCCAGGCTGCAGTGCAATGGTGCCATCTCAGCTGCAACTTCTGCCTCCTGGGTTTAAGCGATTCTCCTGCCTCAGCCTCCCAGGTAGCCGGGATTACAGGCATGCACCACCACACCCAGCTAATTTTTGTATTTTTAGTAGAGACAGGGTTTCACCATATTGGCCAGGCTGGTCTCGAACCCCTGGCCTCAAGTGATCCGCCTGCCTTGGCCTCAAAATGCTAGGATTACAGGCGTGAGCCACCACGTCCAGCAGTTAATAATTTTTTTAAATGTTGTTAATGTCCTTAAGTAATTTTTTAAAAATTTTATTGTATTTTTTTTTTTTTTGAGACAGAGTCTTGCTTGCCCTGTCGCCCAGGCTAGAGTGCAGTGGTGCGATCTCGGCTAACTGCAATCTCTGCCTCCCAGGTTCAAGCAATTCTCCTACCTCAGCCTCCTGAGTAGCTGGGATTACATGTGCACGCCACCACACCTGGCTAATTTTTGTATTTTTAGTAGAGACAGGGTTTCACCGTGTTGGTCAGGTTGATCTCGAACTCCTGACCTCGTGGTCCACCCGTCTCGGCCTCCCAAAGTTCTGGGATTACAGGCATGAGCCACCGGGCCCAGCTGTCCTTAAGTAATTTTTCTAATGCTTTATCAGTGTATCCTTGGACAAATTTAATTTTAATTTTATTTTTAGAAACAGAAACAGGAGAAAATCTTCTTTTGATACAGTAAGTAATATTCAAATTATATTTAAAAAGTTATAAATTAATATTATTTTTATTTCTTATTTTTGTGTTCCTTTTGTGGAATACTTAGGATTAATTCAGAAAACCAGTTATTTGCAGAATTTAGTATACTGCTATATCTGATTAATATGGCCATTGTACTGTTTATAGTTTTAATAGTGATGAAACAATATAATATATAAACCTTTATATTTATTAACTAATATAAAACAATAGATAGCAAGTCTTTAGTTTTTTTATTAAATTAATCTTTCTTATAATATTATTACATAGAAAGGCAAATACCAGTAGCTCTTCAACAAATGAAAAGATGGTTATCTTTATTCATCATAAGAGAAGTACAGATTTGATTATATTGAGATATTTTTGGCTGGACGTGGTGGCTCATGCCTGTAATCCGAGCACTTTGGGAGGCCGAGGCTGGTGGATCACCTGAGGTCAGGAGTTCGAGACCAGCCTGGCCAACATGATGAAACCCCATCTCTACTAAAAAAATACAAAAAATTGGCCGGGCACAGTGGCTTACGCCTGTAAACCAAGCACTTAGGGAGGCTGAGGCGGGTAGATCACGAGGTCAGGAGATCGAGACCATCCTGGCCAACGTGGTGAAACCCCATCTCTACTAAGAATACAAAAATTAGCCAGGTGTTGTGGTGGGCGCCTGTAGTCCCAGCTACTCAGGAGGTTGAGGTAGGAGAATCACTTGAATCTGGGAGGTAGAGGTTGCAGTGAGCCGAGATCATGCCACTGCACTCCAGCCTGGGCGACAGAGCGAGACTCCGTCTCAAAAAAACAAAAAAAACCCCAAAAAATTAGCCAGGTATGGTGGCACTGTGCCTGTAATCCCAGCTACTCAGGAGGCCAAGGCACGAGAATCGCTTGAACCCGGGAGGCGGAGGTTGCAGTGAGCTGAGATCATGCCCCTGCACTCCAACCTGGGCAGCAGAGCGAGACTCTGTCTCCAAAAAAGAGGTTTTTTTGCTGTTGTTGTTGTTTGTTTGTTGTTTAGACGGCATCTTCCTCTGTCGCCAGGCTGGAGTGCAATGGTACGATCTTGGCTCTCTGCAACCTCCGCCTCCTGGGTTCAAGTGATTCTCCTGCTTCAGCCTCCCGAGTAGCTGGGACTACAGGCGCCTGCCACCACGCCTGGCTAATTTTGTTGTATTTTTAGTAGACACGGGGTTTCACCATGTTGGCCAGAATTGTCTTGGTCTCTTGACCTCGTGATCCGCCCACTTTGGCCTCCTAAAGTGCTGGGATTACAGGCGTGAGCCACTGTGCCTGGCCTAAAAAAGAGATATTTTTATCCAGTCAGTTAAGGAAATAGGTAAAAAGTCACTCCGTGCTAGTGGGAGTATAATTTGTAGAAGGCAACTTGGTAATATCGTTCAGAATTTTAAATGCACATGCCCTTTGACTTCAGTTTTATGTCTGGAAATTATCTCACCAATATACGTGTAAATGTTTGAAATGATGAATAGATAAGGTTATTCACAGCAGCGTTTATTATAATAGTAAACGTTGTGAAAGGTCCTGAAGGTCCATCAGTAAGGGCCTGGTTAAATAAATTGTACATTTTCCATATAATTGACTATGCAGCCGGAAAAAAAATTTTATGCTAAATAGAGAAATCTCTAAGCTATATTACTTAAGATAAAAAGCAAGACGCAAAACAAAGTATATCATGTATTACCACTTGGGTAAAACAGGGAAGAAGAAAAGCGTGTGTGTGTGTGTGTATCTTCTCGTGTGTGTACATGTATAATGTATGTATATGTAGTGACTTATGCATATATATGCATAATATCTCTGGAAGAAACTAGTAAGATTGCCTACAGAGAAAAGAACCTTGCTATAGGTTACATGTTGGCTTTGTGGTTTATTAATTTAAAACCATATGAAAGTATTACCTATTACATTCTTAAAATTGTTGAAACGAAGAAATGGAAGTAATAGTAGTCATGGTTTAGAAAAGGTACAGAATCAACTCCAACAGAACTTAAAGGAAAAATAATTTTTTAAATAAAAAGGTACAGAAGTATATACATTGAAAAGTAAAAATTATTCCCTCTTTCCCTCTTCTTTCTTTTTTGTGAGACGGGGTTTCGCTCTTGTTGGCCAGACTGGAGTATAATGGCATGATCTTGGCTCACTGCAACCTCAGCCTCCTGGGTTCAAGCAATTCTCCTGCCTCAGCCTCCTGAGTAGCTAGGACTACAGGTGTGCACCACTACACCTGGCTAATTTATTTTATTTTTATTATTTTATTTTATTTTATTTTATTTTGAGATAGAGTCTCGCTCCGTCACCCAGGCTGGAGTGCAGTGGCGCCATCTCGGCCCACTGCAAGCTCCGCCTCCTGGTTTCACGCCATTCTCCTGCCTCAGCCTCCCTAGTAGCTGGGACTACAGGCGCCTGCCACCACGCCTGGCTCATTTTTTTGTATTTTTAGTAGAGACAGGGTTTCACTGTGTTAGCCAGGATGGTCTCAATTTCCTGACCTTGTGATCCACCCGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCACCACGCCTGGCCCCCCTTTTTTTTTTTTTTGAGATGGAGTCTTGCTCTGTTGTGCAGGCTGGAGTGCAGTGGCCTAATCTTGGCTCACTGCAACCTCCGCCTCATGGGTTCAAGCAATTCTCCTGCCTCAGCCTCCCGAGTACCTGGGACTACAGGTGCACACCACCACGCCCAGCCAATTTTTGTATTTTTAGTAGAGATGGGGTTTCACCACGTTGGCCAGGCTGGTCTCGAACTCCTGACCTCAGTTTATCTGCCTGCCTTGGCTTCCCAAAGTGCTGGGATTTTAGGCATGAGCCACCACACCCGGCCTAATTTTGTATTTTTAGTAGAAGCGGTGTTTCACCATGTCGGCCAAGCTAGTCTCAAACTCCTGACCTCGGGTGATCTGCCTGCCTTGGCCTCCCAAAGTGATGGGATTACAGACGTGAGCCACCACACCCAGCCTATTCCCTCTTCTTCAGGTGACTCACTCCTTACAGATAACCACTGTTGACAATTTTTGGTGTACTTGCAGAAATTTTTTTTGTATGTCCAAGTGCATGTTTAGCTTTTATTTTTTTAAACAAGACAAAAATGGGATTGCCTAGTAATATGTCTTATAGCACTTTCTATGTTATTACATAAAGATTTACCTCAGTCTGTTACAATCTCTTGTATGGCTGCACCAACATTTAGTGAACTCCCTATTGATGGACACTTGAGTGGTTTCTTTCATGTACTTTTTGTTTTAAGGCTCAAGTTTTAGAAAAGGAATTGCTTTATAGGTCATTTTTTGAAAAAATAAGCAAGCAGTATATATTAAGCATATTTTTCTAGTTTATTTTTTTGAGATGGGGTCTCACTCTGTCACCCAGGCTGGAGTGCAGTGACATGATTATAGTTCATTGCAGCCCTGGACTTCAGGTCTCAAGTGATCCTCCCACTTCACGCTCCCAAAGTGTTGGGATTACAGATGTGAGCCACCACGGCTGGCTGATAATATTCTTGAAATTAGCAAACCATTAAGACCAATCTGAAAAGTATCCTTTAGTTGCCTTGTAATTCCTTCAGCCAGTATTAGATGAGTCTTTTTTTAAAAAGATGTCTTTATAATTTTCTGCATTAGATGAGTCTTTTTTAAAAAGATGTCTTTATAATTTTCTGCTAGTCATTCTATGAAAGTCTTTCACCTTCTGTACTCTTTCAGCTGTTATTGAAAGATTCTTGGTAATTGTTTGGGGTATAGATTATAAAAATACTTTTTAATTTTTAGGAATAAGAAATTAGAAGATAATTACTGTGAAATTACTG
+9	17404	18053	JUNC00000003	1	-	17404	18053	255,0,0	2	75,26	0,623	GTCTCTGCCCCCAGCATCACATGGGTCTTTGTTACAGCACCAGCCAGGGGGTCCAGGAAGACATACTTCTTGTACCTACGGAGGCGACATGGGGGTCAGGCAAGCTGACACCCGCTGTCCTGAGCCCATGTTCCTCTCCCACATCATCAGGGGCACAGTGTGCACTGTGGGGTCCCAGGCCTCCCGAGCCGAGCCACCCCAGTCACCCCCTGGCTCCTGGCCTATGTGCTGTACCTGTGTCTGATGCCCTGGGTCCCCACTAAGCCAGGCCGGGCCTCCCGCCCACACCCCTCGGCCCTGCCCTCTGGCCATACAGGTTCTCGGTGGTGTTGAAGAGCAGCAAGGAGCTGACAGAGCTGATGTTGCTGGGAAGACCCCCAAGTCCCTCTTCTGCATCGTCCTCGGGCTCCGGCTTGGTGCTCACGCACACAGGAAAGTCCTTCAGCTTCTCCTGAGAGGGCCAGGATGGCCAAGGGATGGTGAATATTTGGTGCTGGGCCTAATCAGCTGCCATCCCATCCCAGTCAGCCTCCTCTGGGGGACAGAACCCTATGGTGGCCCCGGCTCCTCCCCAGTATCCAGTCCTCCTGGTGTGTGACAGGCTATATGCGCGGCCAGCAGACCTGCAGGGCCCGCTCGTCCAGGGGGC
+8	27369375	27370080	JUNC00000874	1	+	27369375	27370080	255,0,0	2	52,49	0,656	GCCGACCTCTTGCAATCCAAGTGACATGAGCCATGGGTACGTGACAGTAAAGGTGAGTCAGTTTTGTCTCTCAGTCGGCTAAGTGCTCTCCCACCAGGTCACCTAAAACGACCAGCAGAGACACCCAAGAGGCTGAGCTGTGAGGATCACCTGAACCTGAGCCTGGGAAGTGGAGGTTGCAGTGAGCTGTGATCACACCACTGTGCTCCAGCCTGGGCAACGGAGTGAAACCCTGTCTCAAGAAAAAAAAAAAAAAAAAAGGACCAGCAGTGACATTTGTTAAATATCGAGGGTGGTTGAACATCCACTATTTATAAGGAAATGTTATTTCCCACAAATCTCATTCCTCAGAAATCAGTGAAAGACAGACCCTGTCTCGGATTCTATAAAGCAGTGTGACTGATGTGGCCAAACCCCCATAGAACAGCAGGCTAGGGAGCTTGGGCAGCCTGGGAGGCTAAAGCATGCACACAGCTGGCCTGTGTTGGAGGAGAGCATGTTTTCATTCAACAGGCATTTATCGAACACTTCCTCTGTGTCAGGCACAGGCCAGATACAAGGGGTGCAGTGACGATCAAGACAGACCTGTCCTCAAGGAGCCCACATTGAGGGCAAAGAGAGGGAAAGGTCATTAAAATGTTCTTTTTGTTTTAAAGGGTTATCATAAAGCCAAGGCCACACACAGAGGGCCCTGGCTCGTGGCCT
+7	99077597	99079394	JUNC00001897	1	+	99077597	99079394	255,0,0	2	64,37	0,1760	CATTTATTGTCTTTATTTTTCCAACTTGGTGTGCATATCTGTATACCCAACTTCAGAAATAACAGTGTAGGCCAGGTGCGGTGGCTCACACCTGTAATCCCAGCACTTTGGGATTCCGAGGCGGGTGGATCACATGAGGTCAGTAGTTTGAGACCAGCCTGGCCAATATGGTGAAACCCAGCTCTACTTAAGATGCGAAAATTAGCCTGGTGTGGTGGTGCACGCTTGTAGTCCCAGTTACTTGGGAGGCTGAGGCAGGAGAATCGCTTGAACCCGGGAGGCAGAGGTTGCATTGAGCCGAGATCATGCCGCTGCATTCCAGCCTGGGTGACAGAGCGAGACTCCGTCTCAAAACAAAACAAAACAACAACAAAAATAACAGTGTATTTGAGGGCACCACTCCAAACCCTACTGGGGATGATACGTTATGTAAAATATTTACACCAGGTCACCTTTCTGAAATGGGAAAAATTCTGACTCCTGAAACACATCTGGTCCTAAGGGTTTCACATAAGGGACTGGGGACAGTGAATAGTCGTGTTGTGTTAGGGCTCAGACCGAGACACTTCATGCATTCTTCACTCTCACCTCGTGTTCCTCATCTGTAAGACCTCCAGGAGTTGAGAATTATGGTAGATTTACATTCCAAAGGCTCACTTCTTATGCTCTTGAAAGTCTTTTTCTTTCTTTTCTCTGGAAATGGGTAACTTCTTGGTGTGTAACATTAGTGGGTGCTCAGGAAATGATTTTCAACCAACTTCATTTCTGAACCTTCACTGCTTCCATTCTTTTCAGGAGCCTACCCTGTCCTAGTCCACTGTAGTCTCCCACTGTGGGAGTTGCCTTGCTAAATAGTTTTCTTTCCGGGGTCACATGTTTAACCCTTGCACTGACACTCTTAGCACAGAGTCTTGACCACCTCTGTGGGACTGTCCACCCACCTCTGGATTTTTATAATTTTAATTTTCCCTTTAAAAGCATTACAGGAGCATACATAAGTACATGGAAGGCATAGAAAAGGAGGAGAAGAGGAAAGTGTTCATGGGTAACACTGTTAATATTTTGGAAGGTTTCCTGCCAGTCTTTTTTTCCTATGCCTTTACAAAAGTAGCTGAGCATATTCTATGTGCAATCCTGTAATCTGTTTTAAATGTAACATTATTGTATCAGCATTTCCCCACATAATCAGTAAACGAGCATAATATAATTCCATTGTGTGGTCCATAAAAATATTTCATTGTTTTCCCATGTGGGGACATTGAGCTTGCCTTTGTTTGTTCGTTGGTTTGCTTGTGTATTTATAATGTTTGAATAGATGTTTGAGTATAAATCTTTGCATGAATTTTTGATCATTTCCCAAGATACTTTCCCAAAATCAGAATTTTGAAGTTAAATGAGATGGCACTTTAATCTTTATTGATTACTGAATGATTCTTCAACAAACTTTGTACTGTTTTGCATATCCAATAGTAACATTTTAGAGTGCTTCTTTTTTTTTTTGAGACAGAGTTTCGCTCTTGTTGCCCAGGCAGGAGTACAATGGCAGGATCTCAGCTCACCACAACCTCTGCCTCCCCGGTTCAAGTGATTCTCCTGCCTCAGACTCCTGAGTAGCTGGGATTACAGGCATGTGCCACCACACCTAGCTAATTTTGTATTTTTAGTAGAGACGGGGTTTCGCCATGTTGGTCAGGCTGGTCTCAAACTCCCGATCTCGGGTGACCCGCCCACCTCAGCCTCCCAAAGTGCTGGGATTACAGGTGTGAGCCACTGCACCCAGCTGAGAGTGCTTCTTTA
+7	148909514	148910833	JUNC00002152	1	+	148909514	148910833	255,0,0	2	60,41	0,1278	GATCAGCAGGATTTGGCAGACAGAGATATTCCCACGGATCCCAATTCAGGTGAGAACAAGGTCAGAATGAATCTTGAGGGCAACAAGTGTTTTTCAAAAGGAATCATTAAATTAATAATTTATTGTGTTTATGCAGCTGATACTAAAATTACTTCAGCACTGATTTTTTGTTCTTTGAGACGGAGTCTTGCTCTGTTGTTCAGGCTGGAGTGCAGTGGTGTGATCTCAGCTCACCGCAACCTCCGTTTCCCGGGTTTAAGCGATTCTCCTGCCTCAGCCTCCTGAGTAGCTGAGATTACAGGCATGTGCCACCACACCTGGCTCATTTTTTGTATTTTTAGTAGAGATGGGGTTTCACCATGTTGGTCAGGCTGCTTCTGAACTCCTGGCCTCAAATGATCTGCCCGCCTCAGCCTCCCAAAGTGCTGGGATTACGGGCATGAGCCACCGCGTCCGGCCATTCAGCACTGATTTTTGAACAATAAAAACAACCAAAAAGCATCTAAAAGCTGCATATATTTCTGTGTCAGAAAATTATTTACATTTTCTGGAATTTCTCTTGAGTGAATACTAGTTAGAAGTTTCCCAAGAAACTTGTAGGGAGGGTCTAGGCATTCAGGGAGGCATAAAAGAACAATGGAAGCCCCACCTGCCCACGACCTATGTGTGTGTCCATGTGATTGCAGAGCTGAGAAGTCCAGTGTCATTGCCTGGTAGTTAGGACACAAGTCTCATCCTACCCACCATACCAGGGAGAGAGGATGAGAAGTTGAATCTAGGATTCCTTGTACTTTGTCAACTTCACCACAGAGAAATTGAGGGGACCCAGGGCAGAGGGAAAGCGGGGCCTTCCTGCTTCACTGTGGTTCTTATCAATGACCTCTGGTTGCAGCCTCTGCCATCGCTGGTTTCTAAATCATACCTGTTAGCCATGGCCCTGAGGGCCGTTATCTGGGGCTTTCTTCTGGCCTGTCCTGGCCCCATGGGTTCCTCTGGGGCTAAGTGGCAGAGCTGGGAGATCTGGGTTTTAATCCCGGCCCTGCCACTGCACAGACCTTGGGCTAAGCCTCCTTCTGGCCAACTTGAAATGCATCGAGCAGTGAGAGTCCATTTAGGAAGGGGCTTATGAGGGTAAGGAGGATGGCCTGGAAATGCAACACCGATACCAAAACATGCCCTGCAGGTTGCAGCCACCGCATGGGATGGTTTTCAAACCTTTGATGGGAGGCCGAGTAGAACTGAACAAAATCTAAGACTGCCTTCTTTCCATGACAACAGAGTCTCTCATCTCAGCACATGACATTTTGTCATGGATCAAG
+6	41766612	41767580	JUNC00002625	1	-	41766612	41767580	255,0,0	2	55,13	0,955	CTGGTCAAAGACGACATGGACCCCAATCTTCTCTCGATGATTACGGCCAGACCACCTAGAACATGGATATAAGCTTGTTACTAGTAAAAACTGGCATATATAATCGTAGGTAGAGGTAGGTATTAATAAAACAGGAAACTGAGAAAATTTAACCTTCTGGTTTTGGTTTTCCCATCAATAAAATGGTGGTAATCAATGTTCACACTTATTTATTTATTTATTTATTTATTTATTTATTTATTTTCAGACAGGGTCTCACTCTGTTGCCCAGGCTGCAGTGCAGTGGTGTGATTGATAAAGGCTCACTGCAGCTTCAACCTTCCCGAGCTCAGGTGATCCCCCCACCTCAGCCTCCCGAGTAGGTGGGACTATAGGTGCACACCACCATGCCTGGCTAATTTTTGCATTTTTTGTAGAGACAGGGTTTTAACCATGTTGCCGAGGCTGGTCTCAAACTCCTGAGCTCAAGCAATCTGCCTACCTTGGCCTCCCAAAGTTCTAGGGTTACAGTCATGAGCCACTGCGCCTGGCCCATTTATTTGCTGTTAATGTTCCATGAAGTACTCTAAACCTAGAGATCCCTAAACAAGCAATTCAAGCAACAGTCTTTTTGTAGTTTCCTGCCTGTGCTTCTTTTGCAGATTATTTTATACTTCATCCTGAAGATGAATCTACATAAGTCCAAGCCAGGCTAGAAAATCTTTAAGCACAGACACCCAGTGCAGGCTGGATAATTAATTACACAGGAGCATGAGAGCAAAAGCCCAGTCCTGCTGGGCTCCCACATCAATTTGCCTTAGTGGATGTGAACAGTTTAATCTCCATGGAAAACACGAGGTCTCAAGTGAACAGGCTTCCCCTCCCAACACCATAGAAGACATTTGCCCTCACCAAAGCCTTTGGAGCAGCTATTCCCACAGCTCTCACCCAGCTGGGACTCCCACAGCAAGCTCACCTGAATCTTTTAA
+6	84856456	84862316	JUNC00002772	1	-	84856456	84862316	255,0,0	2	48,53	0,5807	TTTACTCTGCAGCTCATTAACTTGACTTTGGAAATGTCTTATAAGTACCTAGGAAATTGTTAGAAGTGCTGAGTTAATAAAGCTCCTCCTTGAACACTATCTGAAAAATATAAGAAAGCCAACTCACAAAAGGGAACTCTTATATACTGTTGGTGGGAATGTAAGTAAGCACAGCTACTATGGAAAAAAATTATAGAGATTTCTCAAAAAACTAAAAATAGAATTACCGTATTATCTAGCAATCCCACTACTGGGTATCTATCCAAAGGAAAAGAAATTTGTATATCAAACGGACACCTGGCACTTGCATGTTTATTGCAGCACTATTCACAATAGCAAAGATATGGAATCAATCTAAGTTCCCATCAACAGACGAAAAGAAATGTGATATATATACCCAATGGAATACAATTCAGCCTTAAATAAGAGGGAAATAATGTCATACGCAGTTAAATGGATGGAACTGAAGGTCATTATGTTAAGTGAAGTAAGCTAGGCACAGAAAAATACTGCATGTTCTCACTCATGTATGAGAGCTAAAAAACTTGATCTCATGAACACAGAGAATAGAACAATAGATACCAGAGATGGGAGTGGGGGGTTGGGAAGGATGAAGAGAGGTTGGTTATGGGTACAAACATACATACAGTTAGATAGATGAAATAAGTTCTAATGTTTAATAGCAGACCAGGGTGACTGTACTTGGCAACAATATTATGTGTATTTCACGGTAACTAGAAGAGAGAGTTTGAAGTGATACCAAGACATAAAAATGATGAACACTCAAGGTGACAGAAATCCCAATTACCCTGACTTGATTATTACACATTCTTTGCATGTAGCAAACACTCACATGTATCCTATAAATATCTAAATCATTATATATCAATAAAAGGGGGGGAAAATAAATATGAACATTTTTCATGACTAGAAAAAGTCCTTTTAAAATAAAGCTCTATAAAGTCTACACATTTGGAAAAAGGTAGACAATATTATCTTTATACTCTGTGGTTAAGTATGATAGTTTTTATTTAAGAACTATGGCTTAAATATAACATAAGCCTCATAATATCTGTGAAATAAATGTCAGTCTCATATCTTTAGGTAAACTGAAATATATGAACAGATACTTCCATCAAGAAACTAAGAGAAATTGGGAAAAATTTATCTTATCTCTAATGCTGTATTTTTAATGATCAGACTATGCTGATTTCTCTTTAACTCAATTTTACAATATTACATTTATAACGCTGGGAAAATAACATTTATGGCACTATGGCATCATGATACTACTTTTCCTACCTCTTCCAGACTATTCCTTAATACAAAATCACTTGATAGAATGCTTATAGTTGCCCTCTGACACCCTATTCCTATGGAGGATAAGGCCAGAGAGGTTCTCTGTAAGGCTGTATGGATTGTTACTTACGAAAGGCACTTTAAGTATTTTTCATTAACGTAGTCAAATAGTCCTTTTTTTTCTCAGCGATCTGAAGTTAAGTTACAGACTTCATTACATTTTGCCCCTAAATACTTCAAATGCCACTTTGGGAGGTCAAAGTGGGTGGATCACTTGAGGTCAGAAGTTCGAGACCAACCTGGCCAACATGGTGAAACCCCATCTCTACCAAAAATATAAAAATTAGTCAGGCATGGTGGCGTGCACCTGTAATCCCAGCTACTTGGAAGGCTGAGACAGAAGAATCGCTTGAACCTGGGAGGCAGAGGTTGCAGTGAGCTGAGACAGCCTGGGTGACAGAAGGAGACTGTCTCAAAGAAACAAAACCTTCAGATGCTTCAACATACATCTCTTTAGAATAAGGGCATTTCTCACAAAACCATAACATTTTATACCTAAGAAAATTTATAGTAATTTCTTAGTATCTAATAGCCAGTCCATATTCAAACTTCCCGAATTGAGGAATTTATTTTTAATTAAGATGGTATTAAATAATTACACTTGGTGAGATAATTCTTTCTTGATGTTTTTCTCCCTGACCTTCTTTCTTAAAATTGAAGCCTACAGACAAGATGAACACAATAAACATTCATATATTTTTCAACTTGATAACCCAAAAGGTGGCTGATTTGCCACCTTTTTCTTTTTCTTGGATATACCATTTGAAAGTAGATTGTAGATAAAAGTCCTATTTTAATGCTATTTTTATAAGTGACAAACAGTACAGAAGACAGGCCACATGGTAATGCTCATAGATATTCTAAGTAGATATACCTATTTTTCAAAAAGGAAGACACATTTATTTGAGGAAATGCTGATGAGTTGGCAGCATTCCCTATAATAATTCTTAGAAGACTATGCTCCATCAGAGCAAGAGCTGTGTATCTCTCTTTTCATTATTTTATTCCAGTGCCTGATTGTCTATTGACCTGAATCATTATTTGTTAATAAAAGGTCATGGGGCAGGAAGAATGCCTAAGGATAACTGTCAGCTGGGACTTGGGGACTCGGTTCAACATCCAAGTTCAAGTACTAGGGTCCTAATCATGTTAGACATATACCTCACCACCTGTGACAAATTTAGAGACTTATTTGAGAAGTATAGGGACAAACTGTAAAAATATTTATTTATATGGTGTAGTTTTAAAGTAGAAAAATTAAATATATAGTATTATCTTCTTTAAAAAAATTAAATATATAGTATTATCTTCTTTAAAAAAGAAATCAAGCCAATAATAGTTTCCCATTTCTATCACCAAGCTGAAAAAGTTAAAACATTTCCAAATAAAACAATGTTAACTTTAATCTCATTAAAGTCAAAACGAGTTTATTCAAGTCAAAAGATAAAACAGATTTGTCATCTACCTCTTGAGTTGCTATTTTACGATTTAGTTCAGCTACTTTGGAAGAAGAATTTTCTACTGCATTTTGGCAAAGGAGTTTCTCTATTTCTCTCTGATGAGATGCTTTGAGGGATGCAATGTGTGCTGCAGTATCTTCCTTGACCCTACAGAGCAAATGAAAGAAAACCACACATAAAAGTGGCTCTTCAACCTCTAATTCAGAGTTAGCACAAAAACAGTAGAAGAAACCTGGGAATTAAGCAAGGGGAAAATGGGCACCAATATTAACTGCACTGTGTTAGGTGCTTTATACATTACTACTGCCTATGAGTTCTTATGACCAATCCATGAGCTACTATTTACTGAATTTTATAAGGGCAAACTTGAGGAAATAGTGATATATCTCTACTTTACCCCAACCTGAGGCTACACTTTCTGAGGGAATCTAGGGTTCCAAAAGCAAAGCACTTGACATTTGATCTTTTAAGATAAACTCAGATTTCTTGCCCAGATGCTTCTTTGGAAAATCACAGCTGTTCCCTTTAGAATCTCCAATGCCTTGTAGGCAGCATTAACCAATTTTCAACCCATGCATGAGACTCCAGGGCTAGTTGCATATTGTGAGAATGAGACAATCTTTATGGTAATTCTCAGGACATATTATGCTTGTAATATGCAATTAATTAATTGGACTGTAAGTGTGAAAGACTATTCATTTGTCTCTGTGATAAAATTTATTCAGAGGCAGTTCAGTAAATGGTTAAAATTATAAATAAAGATTTGGTATATTTCCTACAGATAAACATTTTCTCTCTTATTGGAAAGACTTTGAAAATAACAGCATTTAACTTAAAGCATGTATTTTAGTAAACTGCTGTTGCTATATATTAATATGGTGCTTCCCAACTATAGACAAATTTATCCATTGCTTATTTCACCATATGAATATTTCTCCATGGAAACAGTGTGAGTAGGGTCCTAGAAAAAGCATAAAAACCTAAATTAAAGCACATATAACTGAATGATAGTAAAAAGTCTAAATATTGGACTAGAAAAGTTTATTCTTCACTTCTCAGGCCCAGACTTATCTAAGAAACAGATTGACAATATTAACAAATTTTAAATGATGTACAGGAAAATTAATTGGAAAACCTGAATTTTAAATTCTTTCCTGGAAAAAATGTGATTCTTCTTAAAGAGCTTCATGCTACTGTCATAATATTCAGGACTTAAGAAACAAGCTTTCCATTTGGTAGCAATATGACTGCCATATGGTAACAACATGACTCACTCACTGCCATGGCTGCCAGTCAATAAGCATTTGTTGGGTGTTTCCCATGCTCATTCTCCTCCTACAGCACATTGCTGTGGGGATGCAAAAGCAGCATAATCTTACACCAGTTCTCAAGGAACTACAAGGCTGTGTATGATTAATCCTTTCTTGGTACATATTAGTAAGTTATGTAGGAGTTGGGGGTAGGAGAAGGCAATTTCCTGGGAAGTTCCACGAAATGTTTTGTGAAGGTAGTACTTATAACAAGAATGAGGAAAAGGCATGAGGAAATGTGACAGATTAGGTAAGTAGAGTAGTATTAGAATAGTGATGAGACTTGAGATGCTAGGCAGAGAAATTTAGTCTTGATATTTAAGGAAGTTGAGAGTCACCGGAGAGCAGAGTGACCCAGGTATATGAGTATTTAAAGAATATTAATGATTTTTAAGCAAGATGACTGGGGAAGGTTAAAAAAAAGGAAGATTGGAGTGGGAAAATTGTATAATGGCAGCACTGGTTTATAAAAGTAAGGAAGACTGGAAAATGGGAAACATTTATACCCAAGGAATAACAGAGATAAAGCTGGGCTGGTAATTAAAATAAATATAGACAGGGAAGGGGAGCTTGGGAAGGGGAACTAATTTACAGGTAAAAAAAGGAAACTTGAGGTATTAGTGATTCCAAGAGAAGCAATCCAATAAATAGTTCTAGGTTTCACATCTGAACTCAGCAAAGAGAACAAGACTTTAAAAGTAAATCTAGAAGTCATGGTTCACAGAAACCGTAAGAATGGGTAAGTTTAGCAACAGAGAAAGAACAGAGAATAAACAGAAAACTAAGAGCTGAATGCTGGTAGAAAGCCAGATTTTTGGGGATGGAGAAGGAAGAAGGGTCAGAATTCAGTAAAAGAAACAGAAAAGGAGTTTTGGTCAGAGCAGCCAAGCCACAGAGGTTAAGGGGAGAAGCCATTTCAGGAAAAGTAGTGTGGTCAACATTGTTGAAGAAAATGAGGAAGGAGAAAATGCCCCTGGACTGGGAAAGCTCATTATTGAGACCAGGTTTAAAAAGGGTGATGATGAGAGCCATATTTAAGAGGAAGTGAGATAGAAATAAAAGAAAATACTATTTGTGAAAAACGGGTGCTAAAAGACAGGATTGGATTGGAACCTGATCAGGTGAAAAAAAGATCTGAGGATGTCAAAAGACTCAGAGGAAAGAGACAGTGGATAGCAAATGGAGATAGTGAGAAGGAAATGGATGAGTATAAAGTTCCTTAAGGCATGAATGGATGTGATCACAAGGTCAATTTGAAGAGTTATTTTTGAAAAGAGGACCTTTTCCTTTTAGACTGCCAACTAGCTTTAACAATAACTGTTTTCTGAAAGCAATCTGTCTATAGCAATAATAAACTATGACATTCCTTTGCTAGAAGAGCCCAAAGAGTCAGCTTATTAATACCATTTGGGTACATGCCAAAAGGCAAGACTGGCGAGCCCAAGGACACAATTTGGGTTGACACAGATGTTCAGGTACATCAGAATTTGGGCCACCAGCAGGTAGAAGACCCTATCCTGAAACAGACACATGCTCAGCAATATAATATAATTTATTAATATTCACTTATGAATTCAGTTCGGGGTCAAACTAATTATCTGGAGGAAATATATCGTATAATTTTTCTATTTTTATCTTTTTATTTTTACAAATATTTATAAATAATTTAACATTTTACCTTCTCATGGAGTTTTCAAATTGATTCATCACTGCTTCAGATTTCATCTTCAG
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/translated_bed_sequences.fa	Wed Feb 05 09:27:54 2014 -0500
@@ -0,0 +1,26 @@
+>JUNC00019210_2 pep:novel chromosome:GRCh37:15:40902460:40907575:1 depth:1
+SYENSEKVRNGRNKKLEDNYCEIT
+>JUNC00019210_3 pep:novel chromosome:GRCh37:15:40902461:40907549:1 depth:1
+HMKIVRKSEMEGIRN
+>JUNC00000003_1 pep:novel chromosome:GRCh37:9:17406:18053:-1 depth:1
+APWTSGPCRYKKYVFLDPLAGAVTKTHVMLGAE
+>JUNC00000003_2 pep:novel chromosome:GRCh37:9:17438:18052:-1 depth:1
+PPGRAGPAGTRSMSSWTPWLVL
+>JUNC00000003_3 pep:novel chromosome:GRCh37:9:17404:18051:-1 depth:1
+PLDERALQVQEVCLPGPPGWCCNKDPCDAGGRD
+>JUNC00000874_2 pep:novel chromosome:GRCh37:8:27369376:27370079:1 depth:1
+PTSCNPSDMSHGYVTVKGYHKAKATHRGPWLVA
+>JUNC00001897_1 pep:novel chromosome:GRCh37:7:99077597:99079392:1 depth:1
+HLLSLFFQLGVHICIPNFRNNSVSHCTQLRVLL
+>JUNC00001897_2 pep:novel chromosome:GRCh37:7:99077598:99079360:1 depth:1
+IYCLYFSNLVCISVYPTSEITV
+>JUNC00002152_1 pep:novel chromosome:GRCh37:7:148909514:148910831:1 depth:1
+DQQDLADRDIPTDPNSGENKSLSSQHMTFCHGS
+>JUNC00002152_2 pep:novel chromosome:GRCh37:7:148909515:148910811:1 depth:1
+ISRIWQTEIFPRIPIQVRTRVSHLST
+>JUNC00002625_1 pep:novel chromosome:GRCh37:6:41766614:41767580:-1 depth:1
+LKDSGGLAVIIERRLGSMSSLT
+>JUNC00002625_3 pep:novel chromosome:GRCh37:6:41766612:41767578:-1 depth:1
+KRFRWSGRNHREKIGVHVVFDQ
+>JUNC00002772_1 pep:novel chromosome:GRCh37:6:84856497:84862316:-1 depth:1
+LKMKSEAVMNQFENSMRRYL
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/tool_dependencies.xml	Wed Feb 05 09:27:54 2014 -0500
@@ -0,0 +1,6 @@
+<?xml version="1.0"?>
+<tool_dependency>
+    <package name="biopython" version="1.62">
+        <repository changeset_revision="3e82cbc44886" name="package_biopython_1_62" owner="biopython" toolshed="http://toolshed.g2.bx.psu.edu" />
+    </package>
+</tool_dependency>
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/translate_bed_sequences.py	Wed Feb 05 09:27:54 2014 -0500
@@ -0,0 +1,255 @@
+#!/usr/bin/env python
+"""
+#
+#------------------------------------------------------------------------------
+#                         University of Minnesota
+#         Copyright 2014, Regents of the University of Minnesota
+#------------------------------------------------------------------------------
+# Author:
+#
+#  James E Johnson
+#
+#------------------------------------------------------------------------------
+"""
+
+"""
+Input:  BED file (12 column) + 13th sequence column appended by extract_genomic_dna
+Output: Fasta of 3-frame translations of the spliced sequence
+  
+"""
+
+import sys,re,os.path
+import optparse
+from optparse import OptionParser
+from Bio.Seq import reverse_complement, transcribe, back_transcribe, translate
+
+class BedEntry( object ):
+  def __init__(self, line):
+    self.line = line
+    try:
+      (chrom,chromStart,chromEnd,name,score,strand,thickStart,thickEnd,itemRgb,blockCount,blockSizes,blockStarts,seq) = line.split('\t')[0:13]
+      self.chrom = chrom
+      self.chromStart = int(chromStart)
+      self.chromEnd = int(chromEnd)
+      self.name = name
+      self.score = int(score)
+      self.strand = strand
+      self.thickStart = int(thickStart)
+      self.thickEnd = int(thickEnd)
+      self.itemRgb = itemRgb
+      self.blockCount = int(blockCount)
+      self.blockSizes = [int(x) for x in blockSizes.split(',')]
+      self.blockStarts = [int(x) for x in blockStarts.split(',')]
+      self.seq = seq
+    except Exception, e:
+      print >> sys.stderr, "Unable to read Bed entry" % e
+      exit(1)
+  def get_splice_junctions(self): 
+    splice_juncs = []
+    for i in range(self.blockCount  - 1):
+      splice_junc = "%s:%d_%d" % (self.chrom, self.chromStart + self.blockSizes[i], self.chromStart + self.blockStarts[i+1])
+      splice_juncs.append(splice_junc)
+    return splice_juncs
+  def get_exon_seqs(self):
+    exons = []
+    for i in range(self.blockCount):
+      # splice_junc = "%s:%d_%d" % (self.chrom, self.chromStart + self.blockSizes[i], self.chromStart + self.blockStarts[i+1])
+      exons.append(self.seq[self.blockStarts[i]:self.blockStarts[i] + self.blockSizes[i]])
+    if self.strand == '-':  #reverse complement
+      exons.reverse()
+      for i,s in enumerate(exons):
+        exons[i] = reverse_complement(s)
+    return exons
+  def get_spliced_seq(self):
+    seq = ''.join(self.get_exon_seqs())
+    return seq
+  def get_translation(self,sequence=None):
+    translation = None
+    seq = sequence if sequence else self.get_spliced_seq()
+    if seq:
+      seqlen = len(seq) / 3 * 3;
+      if seqlen >= 3:
+        translation = translate(seq[:seqlen])
+    return translation
+  def get_translations(self):
+    translations = []
+    seq = self.get_spliced_seq()
+    if seq:
+      for i in range(3):
+        translation = self.get_translation(sequence=seq[i:])
+        if translation:
+          translations.append(translation)
+    return translations
+  ## [[start,end,seq],[start,end,seq],[start,end,seq]]
+  ## filter: ignore translation if stop codon in first exon after ignore_left_bp
+  def get_filterd_translations(self,untrimmed=False,filtering=True,ignore_left_bp=0,ignore_right_bp=0):
+    translations = [None,None,None]
+    seq = self.get_spliced_seq()
+    ignore = (ignore_left_bp if self.strand == '+' else ignore_right_bp) / 3
+    block_sum = sum(self.blockSizes)
+    exon_sizes = self.blockSizes
+    if self.strand == '-':
+      exon_sizes.reverse()
+    splice_sites = [sum(exon_sizes[:x]) / 3 for x in range(1,len(exon_sizes))]
+    junc = splice_sites[0] if len(splice_sites) > 0 else exon_sizes[0]
+    if seq:
+      for i in range(3):
+        translation = self.get_translation(sequence=seq[i:])
+        if translation:
+          tstart = 0
+          tstop = len(translation)
+          if not untrimmed:
+            tstart = translation.rfind('*',0,junc) + 1
+            stop = translation.find('*',junc)
+            tstop = stop if stop >= 0 else len(translation)
+          if filtering and tstart > ignore:
+            continue
+          trimmed = translation[tstart:tstop]
+          #get genomic locations for start and end 
+          offset = (block_sum - i) % 3
+          if self.strand == '+':
+            chromStart = self.chromStart + i + (tstart * 3)
+            chromEnd = self.chromEnd - offset - (len(translation) - tstop) * 3
+          else:
+            chromStart = self.chromStart + offset + (len(translation) - tstop) * 3
+            chromEnd = self.chromEnd - i - (tstart * 3)
+          translations[i] = [chromStart,chromEnd,trimmed]
+    return translations
+  def get_seq_id(self,seqtype='unk:unk',reference='',frame=None):
+    ## Ensembl fasta ID format
+    # >ID SEQTYPE:STATUS LOCATION GENE TRANSCRIPT
+    # >ENSP00000328693 pep:splice chromosome:NCBI35:1:904515:910768:1 gene:ENSG00000158815:transcript:ENST00000328693 gene_biotype:protein_coding transcript_biotype:protein_coding
+    frame_name = ''
+    chromStart = self.chromStart
+    chromEnd = self.chromEnd
+    strand = 1 if self.strand == '+' else -1
+    if frame != None:
+      block_sum = sum(self.blockSizes)
+      offset = (block_sum - frame) % 3
+      frame_name = '_' + str(frame + 1)
+      if self.strand == '+':
+        chromStart += frame
+        chromEnd -= offset
+      else:
+        chromStart += offset
+        chromEnd -= frame
+    location = "chromosome:%s:%s:%s:%s:%s" % (reference,self.chrom,chromStart,chromEnd,strand)
+    seq_id = "%s%s %s %s" % (self.name,frame_name,seqtype,location)
+    return seq_id
+  def get_line(self, start_offset = 0, end_offset = 0):
+    if start_offset or end_offset:
+      s_offset = start_offset if start_offset else 0
+      e_offset = end_offset if end_offset else 0
+      if s_offset > self.chromStart:
+        s_offset = self.chromStart
+      chrStart = self.chromStart - s_offset
+      chrEnd = self.chromEnd + e_offset
+      blkSizes = self.blockSizes
+      blkSizes[0] += s_offset
+      blkSizes[-1] += e_offset
+      blkStarts = self.blockStarts
+      for i in range(1,self.blockCount):
+        blkStarts[i] += s_offset
+      items = [str(x) for x in [self.chrom,chrStart,chrEnd,self.name,self.score,self.strand,self.thickStart,self.thickEnd,self.itemRgb,self.blockCount,','.join([str(x) for x in blkSizes]),','.join([str(x) for x in blkStarts])]]
+      return '\t'.join(items) + '\n'
+    return self.line
+
+def __main__():
+  #Parse Command Line
+  parser = optparse.OptionParser()
+  parser.add_option( '-i', '--input', dest='input', help='BED file (tophat junctions.bed) with sequence column added' )
+  parser.add_option( '-o', '--output', dest='output', help='Translations of spliced sequence')
+  parser.add_option( '-b', '--bed_format', dest='bed_format', action='store_true', default=False, help='Append translations to bed file instead of fasta'  )
+  parser.add_option( '-S', '--seqtype', dest='seqtype', default='pep:splice', help='SEQTYPE:STATUS for fasta ID line'  )
+  parser.add_option( '-R', '--reference', dest='reference', default=None, help='Genome Reference Name for fasta ID location '  )
+  parser.add_option( '-Q', '--score_name', dest='score_name', default=None, help='include in the fasta ID line score_name:score '  )
+  parser.add_option( '-l', '--leading_bp', dest='leading_bp', type='int', default=None, help='leading number of base pairs to ignore when filtering' )
+  parser.add_option( '-t', '--trailing_bp', dest='trailing_bp', type='int', default=None, help='trailing number of base pairs to ignore when filtering' )
+  parser.add_option( '-U', '--unfiltered', dest='filtering', action='store_false', default=True, help='Do NOT filterout translation with stop codon in the first exon'  )
+  parser.add_option( '-u', '--untrimmed', dest='untrimmed', action='store_true', default=False, help='Do NOT trim from splice site to stop codon'  )
+  parser.add_option( '-L', '--min_length', dest='min_length', type='int', default=None, help='Minimun length (to first stop codon)'  )
+  parser.add_option( '-M', '--max_stop_codons', dest='max_stop_codons', type='int', default=None, help='Filter out translations with more than max_stop_codons'  )
+  parser.add_option( '-d', '--debug', dest='debug', action='store_true', default=False, help='Turn on wrapper debugging to stdout'  )
+  (options, args) = parser.parse_args()
+  # Input files
+  if options.input != None:
+    try:
+      inputPath = os.path.abspath(options.input)
+      inputFile = open(inputPath, 'r')
+    except Exception, e:
+      print >> sys.stderr, "failed: %s" % e
+      exit(2)
+  else:
+    inputFile = sys.stdin
+  # Output files
+  outFile = None
+  if options.output == None:
+    #write to stdout
+    outFile = sys.stdout
+  else:
+    try:
+      outPath = os.path.abspath(options.output)
+      outFile = open(outPath, 'w')
+    except Exception, e:
+      print >> sys.stderr, "failed: %s" % e
+      exit(3)
+  leading_bp = 0
+  trailing_bp = 0
+  if options.leading_bp:
+    if options.leading_bp >= 0:
+      leading_bp = options.leading_bp
+    else:
+      print >> sys.stderr, "failed: leading_bp must be positive"
+      exit(5)
+  if options.trailing_bp:
+    if  options.trailing_bp >= 0:
+      trailing_bp = options.trailing_bp
+    else:
+      print >> sys.stderr, "failed: trailing_bp must be positive"
+      exit(5)
+  # Scan bed file 
+  try:
+    for i, line in enumerate( inputFile ):
+      if line.startswith('track'):
+        if outFile and options.bed_format:
+          outFile.write(line)
+        continue
+      entry = BedEntry(line)
+      strand = 1 if entry.strand == '+' else -1
+      translations = entry.get_translations()
+      if options.debug:
+        exon_seqs = entry.get_exon_seqs()
+        exon_sizes = [len(seq) for seq in exon_seqs]
+        splice_sites = [sum(exon_sizes[:x]) / 3 for x in range(1,len(exon_sizes))]
+        print >> sys.stderr, entry.name
+        print >> sys.stderr, line.rstrip('\r\n')
+        print >> sys.stderr, "exons:  %s" % exon_seqs
+        print >> sys.stderr, "%s" % splice_sites
+        for i,translation in enumerate(translations):
+          print >> sys.stderr, "frame %d:  %s" % (i+1,translation)
+          print >> sys.stderr, "splice:   %s" % (''.join(['^' if (((j*3)+i)/3) in splice_sites else '-' for j in range(len(translation))]))
+        print >> sys.stderr, ""
+      if options.bed_format:
+        tx_entry  = "%s\t%s\n" % (line.rstrip('\r\n'),'\t'.join(translations))
+        outFile.write(tx_entry)
+      else:
+        translations = entry.get_filterd_translations(untrimmed=options.untrimmed,filtering=options.filtering,ignore_left_bp=leading_bp,ignore_right_bp=trailing_bp)
+        for i,tx in enumerate(translations):
+          if tx:
+            (chromStart,chromEnd,translation) = tx
+            if options.min_length != None and len(translation) < options.min_length:
+              continue
+            if options.max_stop_codons != None and translation.count('*') > options.max_stop_codons:
+              continue
+            frame_name = '_%s' % (i + 1)
+            location = "chromosome:%s:%s:%s:%s:%s" % (options.reference,entry.chrom,chromStart,chromEnd,strand)
+            score = " %s:%s" % (options.score_name,entry.score) if options.score_name else ''
+            seq_id = "%s%s %s %s%s" % (entry.name,frame_name,options.seqtype,location, score)
+            outFile.write(">%s\n" % seq_id)
+            outFile.write(translation)
+            outFile.write('\n')
+  except Exception, e:
+    print >> sys.stderr, "failed: Error reading %s - %s" % (options.input if options.input else 'stdin',e)
+
+if __name__ == "__main__" : __main__()
+
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/translate_bed_sequences.xml	Wed Feb 05 09:27:54 2014 -0500
@@ -0,0 +1,102 @@
+<?xml version="1.0"?>
+<tool id="translate_bed_sequences" name="Translate BED Sequences" version="0.0.1">
+  <description>3 frame translation of BED augmented with a sequence column</description>
+  <requirements>
+    <requirement type="package" version="1.62">biopython</requirement>
+    <requirement type="python-module">Bio</requirement>
+  </requirements>
+  <command interpreter="python">translate_bed_sequences.py  --input "$input" 
+  #if $reference:
+   --reference $reference
+  #else:
+   --reference ${input.metadata.dbkey}
+  #end if
+  #if $seqtype:
+    --seqtype $seqtype
+  #end if
+  #if $score_name:
+    --score_name $score_name
+  #end if
+  #if $filter.filterseqs == 'yes':
+    #if $filter.leading_bp:
+      --leading_bp $filter.leading_bp
+    #end if
+    #if $filter.trailing_bp:
+      --trailing_bp $filter.trailing_bp
+    #end if
+  #else:
+    --unfiltered
+  #end if
+  #if $trim.trimseqs == 'no':
+    --untrimmed
+    #if $trim.max_stop_codons.__str__ != '':
+      --max_stop_codons $trim.max_stop_codons
+    #end if
+  #end if
+  #if $min_length:
+   --min_length $min_length 
+  #end if
+  --output "$output"
+  </command>
+  <inputs>
+    <param name="input" type="data" format="bed" label="BED file with added sequence column" 
+           help="Output from 'Extract Genomic DNA' run on tophat junctions.bed "/> 
+    <param name="reference" type="text" value="" optional="true" label="Genome reference name"
+           help="By default, the database metadata will be used."/>
+    <param name="seqtype" type="text" value="" optional="true" label="The SEQTYPE:STATUS to include in the fasta ID lines"
+           help="For example:  pep:splice"/>
+    <param name="score_name" type="text" value="" optional="true" label="Add the bed score field fasta ID line with this tag name"
+           help="For example:  with the tag name 'depth' and bed score 12:   depth:12"/>
+    <conditional name="filter">
+      <param name="filterseqs" type="select" label="Filter out translations with stop codons before the splice site">
+        <option value="yes" selected="true">Yes</option>
+        <option value="no">No</option>
+      </param>
+      <when value="yes">
+        <param name="leading_bp" type="integer" value="" min="0" optional="true" label="Stop codon filtering start position base pairs" 
+               help="Do not reject translation is stop_codons are within base pairs of the BED start position for positive strand"/>
+        <param name="trailing_bp" type="integer" value="" min="0" optional="true" label="Stop codon filtering end position base pairs" 
+               help="Do not reject translation is stop_codons are within base pairs of the BED end position for negative strand"/>
+      </when>
+      <when value="no"/>
+    </conditional>
+    <conditional name="trim">
+      <param name="trimseqs" type="select" label="Trim translations to stop codons">
+        <option value="yes" selected="true">Yes</option>
+        <option value="no">No</option>
+      </param>
+      <when value="no">
+        <param name="max_stop_codons" type="integer" value="" min="0" optional="true" label="Maximum number of stop codons allowed in a translation to be reported"/>
+      </when>
+    </conditional>
+    <param name="min_length" type="integer" value="" min="0" optional="true" label="Minimum length of a translation to be reported"/>
+  </inputs>
+  <stdio>
+    <exit_code range="1:" level="fatal" description="Error" />
+  </stdio>
+  <outputs>
+    <data name="output" metadata_source="input" format="fasta" label="${tool.name} on ${on_string}">
+      <filter>'found' in str(outputs)</filter>
+    </data>
+  </outputs>
+  <tests>
+    <test>
+      <param name="input" value="Extract_Genomic_DNA.bed" ftype="bed" dbkey="hg19"/>
+      <param name="reference" value="GRCh37"/>
+      <param name="seqtype" value="pep:novel"/>
+      <param name="score_name" value="depth"/>
+      <output name="output" file="translated_bed_sequences.fa"/>
+    </test>
+  </tests>
+  <help>
+**Translate BED Sequences**
+
+This tool takes a BED input file that has been processed 
+by the Galaxy tool "Extract Genomic DNA" to add a 13th column with the transcript sequence.
+
+It generates a peptide fasta file with the 3-frame translations of the spliced sequence 
+defined by each entry in the input BED file.
+
+
+  </help>
+</tool>